| quality of life of mothers of children with congenital syndrome affected by zika virus. | to investigate how mothers of children with congenital syndrome (microcephaly) associated with zika virus perceive their quality of life. | 2021 | 32844897 |
| il-22 hinders antiviral t cell responses and exacerbates zikv encephalitis in immunocompetent neonatal mice. | the zika virus (zikv) outbreak that occurred in multiple countries was linked to increased risk of nervous system injuries and congenital defects. however, host immunity- and immune-mediated pathogenesis in zikv infection are not well understood. interleukin-22 (il-22) is a crucial cytokine for regulating host immunity in infectious diseases. whether il-22 plays, a role in zikv infection is unknown. | 2020 | 32843067 |
| in vitro inhibition of zika virus replication with poly(sodium 4-styrenesulfonate). | zika virus (zikv) is an emerging mosquito-borne pathogen associated with microcephaly and other congenital abnormalities in newborns as well as neurologic complications in adults. the explosive transmission of the virus in the last ten years put it in the limelight and improved our understanding of its biology and pathology. currently, no vaccine or drugs are available to prevent or treat zikv infections. knowing the potential of flaviviruses to broaden their geographic distribution, as observed ... | 2020 | 32842540 |
| n-glycosylation in the pre-membrane protein is essential for the zika virus life cycle. | asparagine (n)-linked protein glycosylation plays an important role in protein synthesis and modification. two zika virus (zikv) structural proteins, the pre-membrane (prm) and envelope (e) protein are n-glycosylated. the prm protein of all zikv strains contains a single n-linked glycosylation site, while not all strains contain an n-linked site in the e protein. our aim was to examine the impact of prm and e n-linked glycosylation on zikv infectivity and cell trafficking. using a zikv infectiou ... | 2020 | 32842538 |
| requests for medication abortion support in brazil during and after the zika epidemic. | brazil declared a public health emergency during the zika virus epidemic, recommending avoiding pregnancy. this study analyses requests received by women help women for information about and support for self-managed medication abortion (ma) in brazil during and after the zika epidemic to understand how zika may have impacted requests to the service. this analysis considered 20,609 requests for ma support received between january 2016 and june 2017. reasons for seeking an abortion were analysed a ... | 2020 | 32841056 |
| a model partnership for communication and dissemination of scientific recommendations for pregnant women during the emergency response to the zika virus outbreak: mothertobaby and the centers for disease control and prevention. | during the zika virus (zikv) outbreak, an urgent need existed for strong partnerships to disseminate zika-related information to pregnant women and women of child-bearing age. | 2020 | 32840950 |
| whole virus detection using aptamers and paper-based sensor potentiometry. | paper-based sensors, microfluidic platforms, and electronics have attracted attention in the past couple of decades because they are flexible, can be recycled easily, environmentally friendly, and inexpensive. here we report a paper-based potentiometric sensor to detect the whole zika virus with a minimum sensitivity of 0.26 nv/zika and a minimum detectable signal (mds) of 2.4x107 zika. our paper sensor works very similar to a p-n junction where a junction is formed between two different regions ... | 2020 | 32838210 |
| are hospitals our friends? an exploratory study on the role of facebook in hospital organizations' dialogic communication. | rooted in the dialogic public relations model, the aim of this paper was to analyze the way in which hospital organizations use facebook to communicate and build relationships with their patients and followers. using a content analysis of facebook profiles, there is a reflection on how dialogic principles are applied in the management of facebook by hospitals in portugal. special attention will be paid to communication regarding the zika virus, which was of public interest at the time the empiri ... | 2020 | 32835646 |
| the state of one health research across disciplines and sectors - a bibliometric analysis. | there is a growing interest in one health, reflected by the rising number of publications relating to one health literature, but also through zoonotic disease outbreaks becoming more frequent, such as ebola, zika virus and covid-19. this paper uses bibliometric analysis to explore the state of one health in academic literature, to visualise the characteristics and trends within the field through a network analysis of citation patterns and bibliographic links. the analysis focuses on publication ... | 2020 | 32835067 |
| fluorescent quantum dots-zika virus hybrid nanoconjugates for biolabeling, bioimaging, and tracking host-cell interactions. | the earliest possible diagnosis and understanding of the infection mechanisms play a crucial role in the outcome of fighting viral diseases. thus, we designed and developed for the first time, novel bioconjugates made of ag-in-s@zns (zais) fluorescent quantum dots coupled with zika virus via covalent amide bond with carboxymethylcellulose (cmc) biopolymer for labeling and bioimaging the virus-host cell interactions mechanisms through confocal laser scanning microscopy. this work offers relevant ... | 2020 | 32834256 |
| teratogen update: zika virus and pregnancy. | zika virus was first identified in uganda in 1947 but received little attention until 2015 when a large outbreak of zika virus illness followed by an increased number of babies born with microcephaly occurred in brazil. zika virus spread rapidly throughout the americas, and in 2016 was identified as a cause of microcephaly and other serious birth defects. since that time, much has been learned about the zika virus. the virus is primarily spread by the bite of aedes species mosquitoes; however, o ... | 2020 | 32830420 |
| a review of the control of aedes aegypti (diptera: culicidae) in the continental united states. | aedes aegypti (l) is an anthropophilic mosquito involved in the transmission of a variety of viral pathogens worldwide including dengue, chikungunya, yellow fever, and zika viruses. this species, native to africa, is well established in the continental u.s. (conus) and occasionally contributes to localized outbreaks of viral diseases. in the last seven decades, mosquito control programs in the conus have been focused on vectors of eastern equine encephalitis, st. louis encephalitis, and west nil ... | 2020 | 32829398 |
| papaya fruit pulp and resulting lactic fermented pulp exert antiviral activity against zika virus. | there are a several emerging and re-emerging rna viruses that are prevalent around the world for which there are no licensed vaccines or antiviral drugs. zika virus (zikv) is an example of an emerging virus that has become a significant concern worldwide because of its association with severe congenital malformations and neurological disorders in adults. several polyphenol-rich extracts from plants were used as nutraceuticals which exhibit potent in vitro antiviral effects. here, we demonstrated ... | 2020 | 32825246 |
| label-free electrochemical biosensors for the determination of flaviviruses: dengue, zika, and japanese encephalitis. | a highly effective way to improve prognosis of viral infectious diseases and to determine the outcome of infection is early, fast, simple, and efficient diagnosis of viral pathogens in biological fluids. among a wide range of viral pathogens, flaviviruses attract a special attention. flavivirus genus includes more than 70 viruses, the most familiar being dengue virus (denv), zika virus (zikv), and japanese encephalitis virus (jev). haemorrhagic and encephalitis diseases are the most common sever ... | 2020 | 32824351 |
| post-fever retinitis - newer concepts. | post-fever retinitis (pfr) is an infectious or para-infectious uveitic entity caused by bacterial or viral agents and seen mainly in tropical countries. systemic symptoms such as joint pain, skin rash are common during the febrile stage. on the basis of only clinical presentation, it is difficult to pin-point the exact etiology for pfr. serological investigations, polymerase chain reaction, and knowledge of concurrent epidemics in the community may help to identify the etiological organism. bact ... | 2020 | 32823394 |
| development of a potent zika virus vaccine using self-amplifying messenger rna. | zika virus (zikv) is the cause of a pandemic associated with microcephaly in newborns and guillain-barre syndrome in adults. currently, there are no available treatments or vaccines for zikv, and the development of a safe and effective vaccine is a high priority for many global health organizations. we describe the development of zikv vaccine candidates using the self-amplifying messenger rna (sam) platform technology delivered by cationic nanoemulsion (cne) that allows bedside mixing and is par ... | 2020 | 32821824 |
| continuous epileptiform discharges during sleep as an evolutionary pattern in patients with congenital zika virus syndrome. | congenital zika virus syndrome (czvs) is associated with severe neurological deficits. clinical characteristics of epilepsy and the electroencephalographic (eeg) pattern in czvs were documented in infancy. in this study, we aimed to describe the eeg findings observed during the follow-up of children with czvs. seventy-six eegs of 55 children (60% female; mean age = 50 months) with confirmed czvs were analyzed, considering the background, interictal, and ictal epileptiform discharges. continuous ... | 2020 | 32820832 |
| enhanced effect of modified zika virus e antigen on the immunogenicity of dna vaccine. | it has been reported worldwide that the zika virus (zikv) could be transmitted through placentas and sexual contact. zikv can also cause guillain-barre syndrome, microcephaly and neurological abnormalities. however, there are no approved vaccines available. we constructed six dna vaccine candidates and tested the immunogenicity. tandem repeated envelope domain ⅲ (ed ⅲ × 3) induced highly total igg and neutralization antibody, as well as cd8+ t cell responses. also, stem region-removed envelope ( ... | 2020 | 32818729 |
| information-accessing behavior during zika virus outbreak, united states, 2016. | we used latent class analysis to examine zika virus-related information-accessing behavior of us residents during the 2016 international outbreak. we characterized 3 classes of information-accessing behavior patterns: universalists, media seekers, and passive recipients. understanding these patterns is crucial to planning risk communication during an emerging health threat. | 2020 | 32818420 |
| zika virus infection, philippines, 2012. | | 2020 | 32818418 |
| differentiation-dependent susceptibility of human muscle cells to zika virus infection. | muscle cells are potential targets of many arboviruses, such as ross river, dengue, sindbis, and chikungunya viruses, that may be involved in the physiopathological course of the infection. during the recent outbreak of zika virus (zikv), myalgia was one of the most frequently reported symptoms. we investigated the susceptibility of human muscle cells to zikv infection. using an in vitro model of human primary myoblasts that can be differentiated into myotubes, we found that myoblasts can be pro ... | 2020 | 32817655 |
| detailed analyses of zika virus tropism in culex quinquefasciatus reveal systemic refractoriness. | the role of culex quinquefasciatus in zika virus transmission has been debated since the epidemic of zika occurred in the americas in 2015 to 2016. the majority of studies have found no evidence that c. quinquefasciatus or other culex species are competent vectors of zika virus, and the few studies that have proposed zika vector status for c. quinquefasciatus have relied predominantly on quantitative real-time pcr (qrt-pcr) for viral detection. we assessed the infectious range of pre- and post-e ... | 2020 | 32817107 |
| diagnostic limitations in congenital zika virus infection. | this is the first documented case of an infant with congenital zika virus infection (zvi) born in ireland. a term infant was delivered with an antenatal diagnosis of severe microcephaly. first trimester bloods confirmed maternal zvi and although the infant did not have zika virus rna or zika-specific igm in her blood or urine, she had multiple clinical features of congenital zvi and zika virus rna was present in the placenta. | 2020 | 32816436 |
| congenital zika virus infection in a birth cohort in vietnam, 2017-2018. | to detect congenital zikv infection (czi) in a birth cohort and among high-risk neonates in vietnam, we collected umbilical cord blood plasma samples of newly delivered babies and peripheral plasma samples of high-risk neonates in nha trang, central vietnam, between july 2017 and september 2018. samples were subjected to serological and molecular tests. of the 2013 newly delivered babies, 21 (1%) were positive for zika virus (zikv) igm and 1,599 (79%) for flavivirus igg. among the 21 zikv igm-po ... | 2020 | 32815502 |
| exploratory analysis of machine learning approaches for surveillance of zika-associated birth defects. | in 2016, centers for disease control and prevention (cdc) established surveillance of pregnant women with zika virus infection and their infants in the u.s. states, territories, and freely associated states. to identify cases of zika-associated birth defects, subject matter experts review data reported from medical records of completed pregnancies to identify findings that meet surveillance case criteria (manual review). the volume of reported data increased over the course of the zika virus out ... | 2020 | 32815300 |
| human neural stem cell systems to explore pathogen-related neurodevelopmental and neurodegenerative disorders. | building and functioning of the human brain requires the precise orchestration and execution of myriad molecular and cellular processes, across a multitude of cell types and over an extended period of time. dysregulation of these processes affects structure and function of the brain and can lead to neurodevelopmental, neurological, or psychiatric disorders. multiple environmental stimuli affect neural stem cells (nscs) at several levels, thus impairing the normal human neurodevelopmental program ... | 2020 | 32806773 |
| evaluation of a health information exchange system for microcephaly case-finding - new york city, 2013-2015. | birth defects surveillance in the united states is conducted principally by review of routine but lagged reporting to statewide congenital malformations registries of diagnoses by hospitals or other health care providers, a process that is not designed to rapidly detect changes in prevalence. health information exchange (hie) systems are well suited for rapid surveillance, but information is limited about their effectiveness at detecting birth defects. we evaluated hie data to detect microcephal ... | 2020 | 32804962 |
| sirna design to silence the 3'utr region of zika virus. | the disease caused by the zika virus (zikv) has positioned itself as one of the main public health problems in mexico. one of the main reasons is it causes microcephaly and other birth defects. the transmission of zikv is through aedes aegypti and ae. albopictus mosquitoes, which are found in a larger space of the national territory. in addition, it can also be transmitted via blood transfusion, sexual relations, and maternal-fetal route. so far, there are no vaccines or specific treatments to d ... | 2020 | 32802864 |
| mapping the diverse structural landscape of the flavivirus antibody repertoire. | flaviviruses are emerging arthropod-borne rna viruses, causing a broad spectrum of life-threatening disease symptoms such as encephalitis and hemorrhagic fever. successful vaccines exist against yellow fever virus, japanese encephalitis virus and tick-borne encephalitis virus. however, vaccine development against other flaviviruses like dengue virus is not straightforward. this is partly because of the high sequence conservation and immunological cross-reactivity among flavivirus envelope glycop ... | 2020 | 32801077 |
| two genetic differences between closely related zika virus strains determine pathogenic outcome in mice. | recent zika virus (zikv) outbreaks and unexpected clinical manifestations of zikv infection have prompted an increase in zikv-related research. here, we identify two strain-specific determinants of zikv virulence in mice. we found that strain h/pf/2013 caused 100% lethality in ifnar1-/- mice, whereas prvabc59 caused no lethality; both strains caused 100% lethality in ifnar1-/-ifngr1-/- double-knockout (dko) mice. deep sequencing revealed a high-frequency variant in prvabc59 not present in h/pf/2 ... | 2020 | 32796074 |
| non-nucleoside inhibitors of zika virus rna-dependent rna polymerase. | zika virus (zikv) remains a potentially significant public health concern because it can cause teratogenic effects, such as microcephaly in newborns and neurological disease, like guillain-barré syndrome. together with efforts to develop a vaccine, the discovery of antiviral molecules is important to control zikv infections and to prevent its most severe symptoms. here, we report the development of small nonnucleoside inhibitors (nnis) of zikv rna-dependent rna polymerase (rdrp) activity. these ... | 2020 | 32796069 |
| robotic high-throughput biomanufacturing and functional differentiation of human pluripotent stem cells. | efficient translation of human induced pluripotent stem cells (hipscs) depends on implementing scalable cell manufacturing strategies that ensure optimal self-renewal and functional differentiation. currently, manual culture of hipscs is highly variable and labor-intensive posing significant challenges for high-throughput applications. here, we established a robotic platform and automated all essential steps of hipsc culture and differentiation under chemically defined conditions. this streamlin ... | 2020 | 32793899 |
| anks4b restricts replication of zika virus by downregulating the autophagy. | infection of zika virus (zikv) has become a severe threaten to global health while no specific drug is available. in this study, we explored the relationship between zikv and a cellular protein, ankyrin repeat and sterile motif domain containing 4b (anks4b). our data revealed that the expression of anks4b in cultured cells and in neonatal mice was downregulated by zikv infection. the reduction of anks4b upon zikv infection was caused by decrease of two hepatocyte nuclear factors hnf1α and hnf4α. ... | 2020 | 32793175 |
| unlike zika, chikungunya virus interferes in the viability of aedes aegypti eggs, regardless of females' age. | chikungunya and zika are arboviruses transmitted by the mosquito aedes aegypti. mosquito fecundity and egg viability are important parameters of vectorial capacity. here we aim to understand, comparatively, the effects of chikungunya virus (chikv) and zika virus (zikv) infections on the fecundity and fertility of young and old aedes aegypti females. using artificial infection blood feeding experiments we observed that both chikv and zikv do not alter the number of eggs laid when compared to unin ... | 2020 | 32788625 |
| diverse types of diterpenoids with an aromatized c ring from the twigs of podocarpus imbricatus. | an ethanol extract of the powdered twigs of podocarpus imbricatus afforded 14 new diterpenoids (1-14), which all share an aromatized c ring. these isolates belong to five diterpenoid types that include abietanes (1-3), semperviranes (4-9), totaranes (10-12), a c-17 norabietane (13), and an icetexane (14). their structures were assigned mainly by analysis of the spectroscopic data, and the absolute configuration of 1 was determined by x-ray crystallography. a biosynthetic pathway for five of the ... | 2020 | 32786878 |
| small molecule inhibitors possibly targeting the rearrangement of zika virus envelope protein. | the recurrent public health threat imposed by zika virus (zikv) in various geographical areas necessitates the immediate development of antiviral compounds or vaccines. flaviviral envelope (e) proteins are essential for host-cell recognition and virion entry. consequently, they represent an important target for antiviral therapy, with the aim of preventing viral spread during early stages of infection. due to conformational rearrangement during entry, flavivirus e proteins present several altern ... | 2020 | 32783901 |
| unlike dengue virus, the conserved 14-23 residues in n-terminal region of zika virus capsid is not involved in lipid interactions. | zika virus capsid protein is involved in multiple essential steps of the viral life cycle. many vital functionalities are attributed to the dynamic n- terminal domain of this protein, which is intrinsically disordered in zikv and among several flaviviruses too. other than genome encapsulation, studies have shown interaction with host lipid droplets to be crucial for replication and maturation. in dengue virus, the molecular basis of such interplay has been studied in detail, and residues within ... | 2020 | 32783888 |
| zika virus-an update on the current efforts for vaccine development. | in 2015, the world witnessed the resurgence and global spread of zika virus (zikv). this arbovirus infection is associated with guillain-barré syndrome in adults and with devastating congenital malformations during pregnancy. despite scientific efforts, the development of a vaccine capable of inducing long-term protection has been challenging. without a safe and efficacious licensed vaccine, control of virus transmission is based on vector control, but this strategy has been shown to be ineffici ... | 2020 | 32780659 |
| structural basis for stat2 suppression by flavivirus ns5. | suppressing cellular signal transducers of transcription 2 (stat2) is a common strategy that viruses use to establish infections, yet the detailed mechanism remains elusive, owing to a lack of structural information about the viral-cellular complex involved. here, we report the cryo-em and crystal structures of human stat2 (hstat2) in complex with the non-structural protein 5 (ns5) of zika virus (zikv) and dengue virus (denv), revealing two-pronged interactions between ns5 and hstat2. first, the ... | 2020 | 32778820 |
| author correction: ahr is a zika virus host factor and a candidate target for antiviral therapy. | an amendment to this paper has been published and can be accessed via a link at the top of the paper. | 2020 | 32778795 |
| neurocognitive impacts of arbovirus infections. | arthropod-borne viruses or arbovirus, are most commonly associated with acute infections, resulting on various symptoms ranging from mild fever to more severe disorders such as hemorrhagic fever. moreover, some arboviral infections can be associated with important neuroinflammation that can trigger neurological disorders including encephalitis, paralysis, ophthalmological impairments, or developmental defects, which in some cases, can lead to long-term defects of the central nervous system (cns) ... | 2020 | 32778106 |
| immuno-informatics-based identification of novel potential b cell and t cell epitopes to fight zika virus infections. | globally, the recent outbreak of zika virus (zikv) in brazil, asia pacific, and other countries highlighted the unmet medical needs. currently, there are neither effective vaccines nor therapeutics available to prevent or treat zikv infection. | 2020 | 32778040 |
| denv and zikv detection in patients with acute febrile syndrome in córdoba, colombia. | to determine simultaneous circulation of denv serotypes and zikv in córdoba, colombia, during 2015 and 2016. | 2020 | 32777585 |
| the association of anti-ganglioside antibodies in the pathogenesis and development of zika-associated guillain-barré syndrome. | zika virus (zikv) has created major outbreaks all over the americas and has caused severe neurological complications. the main neurological complications linked to zikv are guillain-barré syndrome (gbs), encephalitis, myelitis, and microcephaly. we thoroughly searched for published literature on pubmed and found evidence supporting the relationship between zikv and gbs. through april 1, 2020, 429 publications were available on pubmed using the words "zika associated gbs." among these, only four ... | 2020 | 32775065 |
| cellular organelles reorganization during zika virus infection of human cells. | zika virus (zikv) is an enveloped positive stranded rna virus belonging to the genus flavivirus in the family flaviviridae that emerged in recent decades causing pandemic outbreaks of human infections occasionally associated with severe neurological disorders in adults and newborns. the intracellular steps of flavivirus multiplication are associated to cellular membranes and their bound organelles leading to an extensive host cell reorganization. importantly, the association of organelle dysfunc ... | 2020 | 32774331 |
| blueprint of epitope-based multivalent and multipathogenic vaccines: targeted against the dengue and zika viruses. | both dengue virus (denv) and zika virus (zikv) belong to the highly infectious flaviviridae family that has already caused several outbreaks and epidemics in many countries. denv and zikv cause two of the most wide spread mosquito-borne viral diseases in the world, dengue fever (denf) and zika fever (zikf), respectively. in many regions around the world, both of these diseases can outbreak together and can be lethal as well as life-threatening. unfortunately, there is no functional and satisfact ... | 2020 | 32772811 |
| neurologic infections during pregnancy. | neurologic infections during pregnancy represent a significant cause of maternal and fetal morbidity and mortality. immunologic alterations during pregnancy increase the susceptibility of the premature brain to damage. this chapter summarizes the epidemiology, pathophysiology, and clinical manifestations in the pregnant woman and the infant, and the diagnosis, treatment, and prevention of the major viral, parasitic, and bacterial infections known to affect pregnancy. these organisms include herp ... | 2020 | 32768096 |
| anti-zika virus activity and chemical characterization by ultra-high performance liquid chromatography (uplc-dad-uv-ms) of ethanol extracts in tecoma species. | plant species from the genus tecoma are found in tropical and subtropical regions around the world. some of them are grown as ornamental plants and others can be used as medicinal plants. in the present study, ethanolic extracts from trunks and leaves of tecoma species were tested in vitro using assays against the zika virus. | 2020 | 32767975 |
| fatal outcome of chikungunya virus infection in brazil. | chikungunya virus (chikv) emerged in the americas in 2013 and has caused ~2.1 million cases and over 600 deaths. a retrospective investigation was undertaken to describe clinical, epidemiological and virus genomic features associated with deaths caused by chikv in ceará state, northeast brazil. | 2020 | 32766829 |
| unilateral morning glory disc anomaly in a patient with prenatal zika virus exposure. | to report a case of morning glory disc anomaly (mgda) in a pediatric patient with prenatal zika virus (zikv) exposure. | 2020 | 32765899 |
| identification of the viral rna promoter stem loop a (sla)-binding site on zika virus polymerase ns5. | zika virus has recently emerged as an important human pathogen that has spread to more than 60 countries. infection of a pregnant woman with zika virus can cause severe brain malformations in the child such as microcephaly and other birth defects. despite the medical importance of zika virus infection, the mechanism of viral replication, a process commonly targeted by antiviral therapeutics, is not well understood. stem-loop a (sla), located in the 5' untranslated region of the viral genome, act ... | 2020 | 32764551 |
| lights and shadows of torch infection proteomics. | congenital abnormalities cause serious fetal consequences. the term torch is used to designate the most common perinatal infections, where: (t) refers to toxoplasmosis, (o) means "others" and includes syphilis, varicella-zoster, parvovirus b19, zika virus (zikv), and malaria among others, (r) refers to rubella, (c) relates to cytomegalovirus infection, and (h) to herpes simplex virus infections. among the main abnormalities identified in neonates exposed to congenital infections are central nerv ... | 2020 | 32764347 |
| detection of a locally-acquired zika virus outbreak in hidalgo county, texas through increased antenatal testing in a high-risk area. | hidalgo county (hc), located along the texas-mexico border, was listed as a high-risk county for zika virus (zikv) in 2017 by the texas department of state health services, based on its historical presence of dengue. due to its subtropical climate, active binational travel, and population of low socioeconomic status, hidalgo county focused on disease detection activities for the prevention of further transmission. therefore, hidalgo county health and human services enacted public health surveill ... | 2020 | 32764299 |
| the zika virus capsid disrupts corticogenesis by suppressing dicer activity and mirna biogenesis. | zika virus (zikv) causes microcephaly and disrupts neurogenesis. dicer-mediated mirna biogenesis is required for embryonic brain development and has been suggested to be disrupted upon zikv infection. here we mapped the zikv-host interactome in neural stem cells (nscs) and found that dicer is specifically targeted by the capsid from zikv, but not other flaviviruses, to facilitate zikv infection. we identified a capsid mutant (h41r) that loses this interaction and does not suppress dicer activity ... | 2020 | 32763144 |
| contrasted transmission efficiency of zika virus strains by mosquito species aedes aegypti, aedes albopictus and culex quinquefasciatus from reunion island. | zika virus (zikv) is a mosquito-borne flavivirus that recently emerged in the south pacific islands and americas where unprecedented outbreaks were reported. although aedes aegypti is considered to be the main vector for zikv, other mosquito species have been shown to be potential vectors and differences in vector competence with respect to mosquito strain and zikv strain have been demonstrated. in this study we compared the vector competence of three mosquito species aedes albopictus, ae. aegyp ... | 2020 | 32762767 |
| zika virus-induced neuro-ocular pathology in immunocompetent mice correlates with anti-ganglioside autoantibodies. | a severe consequence of adult zika virus (zikv) infection is guillain-barré syndrome (gbs), where autoreactive antibodies attack peripheral and central nervous systems (cns) resulting in neuro-ocular pathology and fatal complications. during virally induced gbs, autoimmune brain demyelination and macular degeneration correlate with low virus neutralization and elevated antibody-mediated infection among fcγ-r bearing cells. the use of interferon-deficient mice for zikv studies limits elucidation ... | 2020 | 32758108 |
| cutaneous vaccination ameliorates zika virus-induced neuro-ocular pathology via reduction of anti-ganglioside antibodies. | zika virus (zikv) causes moderate to severe neuro-ocular sequelae, with symptoms ranging from conjunctivitis to guillain-barré syndrome (gbs). despite the international threat zikv poses, no licensed vaccine exists. as zikv and denv are closely related, antibodies against one virus have demonstrated the ability to enhance the other. to examine if vaccination can confer robust, long-term protection against zikv, preventing neuro-ocular pathology and long-term inflammation in immune-privileged com ... | 2020 | 32758106 |
| zika virus disease and pregnancy outcomes in colombia. | in 2015 and 2016, colombia had a widespread outbreak of zika virus. data from two national population-based surveillance systems for symptomatic zika virus disease (zvd) and birth defects provided complementary information on the effect of the zika virus outbreak on pregnancies and infant outcomes. | 2020 | 32757522 |
| repercussions of zika virus emergency on the health of the population of tocantins state, brazil, 2015 and 2016: a descriptive study. | objective to describe the occurrence of zika virus disease and its complications in the state of tocantins and in its capital, the city of palmas. methods this was a descriptive study using data from health information systems. results incidence of reported zika virus disease cases in 2015 and 2016 was 295.2/100,000 inhabitants and 411.1/100,000 inhab. in the general population, and 5.9/1,000 and 27.8/1,000 live births, respectively. higher risks occurred in women, the 20-39 year age group, muni ... | 2020 | 32756832 |
| normal hearing function in children prenatally exposed to zika virus. | introduction the association between prenatal zika virus infection and hearing alterations in offspring has been the object of some studies, although few have assessed children without microcephaly. however, a current trend to include prenatal zika virus exposure in the group of risk indicators for hearing loss is noted. objective to present a series of 27 children prenatally exposed to the zika virus submitted to multiple hearing assessments over time. methods a cohort of children born to sy ... | 2020 | 32754240 |
| chemical proteomics tracks virus entry and uncovers ncam1 as zika virus receptor. | the outbreak of zika virus (zikv) in 2016 created worldwide health emergency which demand urgent research efforts on understanding the virus biology and developing therapeutic strategies. here, we present a time-resolved chemical proteomic strategy to track the early-stage entry of zikv into host cells. zikv was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on uv exposure at different time points, and ... | 2020 | 32753727 |
| zika virus infection promotes local inflammation, cell adhesion molecule upregulation, and leukocyte recruitment at the blood-brain barrier. | the blood-brain barrier (bbb) largely prevents toxins and pathogens from accessing the brain. some viruses have the ability to cross this barrier and replicate in the central nervous system (cns). zika virus (zikv) was responsible in 2015 to 2016 for a major epidemic in south america and was associated in some cases with neurological impairments. here, we characterized some of the mechanisms behind its neuroinvasion using an innovative in vitro human bbb model. zikv efficiently replicated, was r ... | 2020 | 32753493 |
| proteome-wide zika virus cd4 t cell epitope and hla restriction determination. | zika virus (zikv) is a mosquito-borne pathogen that caused an epidemic in 2015-2016. zikv-specific t cell responses are functional in animal infection models, and helper cd4 t cells promote avid abs in the vaccine context. the small volumes of blood available from field research limit the determination of t cell epitopes for complex microbes such as zikv. the goal of this project was efficient determination of human zikv cd4 t cell epitopes at the whole proteome scale, including validation of re ... | 2020 | 32753403 |
| rt-dpcr in mosquito samples for zikv detection: effects of rna extraction and reverse transcription in target concentration. | zika virus (zikv) is an important arbovirus, responsible for recent outbreaks of guillain barré syndrome and congenital zika syndrome (czs). after thousands of czs cases, zikv is under constant surveillance in brazil. reliable and robust detection techniques are required to minimize the influence of host inhibitors from clinical samples and mosquito pool samples. reverse transcription digital polymerase chain reaction (rt-dpcr) is a technique that allows the accurate quantification of dna target ... | 2020 | 32751540 |
| human virus detection with graphene-based materials. | our recent experience of the covid-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for t ... | 2020 | 32750677 |
| insights on zika virus envelope gene conservation in american outbreaks. | phylogenetic studies with zika virus (zikv) have been conducted in brazil. in this study, we sequenced 8 new sequences of the zikv envelope (e) gene from strains of cases from the paraná and mato grosso do sul states in 2016. a low phylogenetic signal was observed, with more than 40% of unresolved quartets, and the maximum likelihood tree grouped all sequences in the brazilian branches within the asian genotype. in addition, a shannon entropy analysis was conducted, showing a high stability in t ... | 2020 | 32749573 |
| integrated pipeline for the accelerated discovery of antiviral antibody therapeutics. | the emergence and re-emergence of highly virulent viral pathogens with the potential to cause a pandemic creates an urgent need for the accelerated discovery of antiviral therapeutics. antiviral human monoclonal antibodies (mabs) are promising candidates for the prevention and treatment of severe viral diseases, but their long development timeframes limit their rapid deployment and use. here, we report the development of an integrated sequence of technologies, including single-cell mrna-sequence ... | 2020 | 32747832 |
| differential gene expression elicited by zikv infection in trophoblasts from congenital zika syndrome discordant twins. | zika virus (zikv) causes congenital zika syndrome (czs), which is characterized by fetal demise, microcephaly and other abnormalities. zikv in the pregnant woman circulation must cross the placental barrier that includes fetal endothelial cells and trophoblasts, in order to reach the fetus. czs occurs in ~1-40% of cases of pregnant women infected by zikv, suggesting that mothers' infection by zikv during pregnancy is not deterministic for czs phenotype in the fetus. therefore, other susceptibili ... | 2020 | 32745093 |
| interferon stimulated gene 15 promotes zika virus replication through regulating jak/stat and isgylation pathways. | zika virus is an emergent arbovirus that has caused a public health emergency in south america. zika virus infection is known to cause microcephaly and other congenital defects and guillain-barré syndrome. unfortunately no direct antiviral treatments are available at present. ifn-stimulated gene 15 (isg15) is one of the most upregulated host genes following type i interferon treatment or virus infections. isg15 has been shown to have antiviral effect on a wide variety of viruses although pro-hcv ... | 2020 | 32738280 |
| automated application of low energy electron irradiation enables inactivation of pathogen- and cell-containing liquids in biomedical research and production facilities. | ionizing radiation is widely used to inactivate pathogens. it mainly acts by destroying nucleic acids but causes less damage to structural components like proteins. it is therefore highly suited for the sterilization of biological samples or the generation of inactivated vaccines. however, inactivation of viruses or bacteria requires relatively high doses and substantial amounts of radiation energy. consequently, irradiation is restricted to shielded facilities-protecting personnel and the envir ... | 2020 | 32732876 |
| lab-on-paper for all-in-one molecular diagnostics (lamda) of zika, dengue, and chikungunya virus from human serum. | several tropical fever viruses transmitted by mosquitoes including zika, dengue, and chikungunya, are becoming a serious problem in global public health. simple diagnostic tools in early stages are strongly required to monitor and prevent these diseases. paper diagnostic platforms can provide a solution for these needs, with integration of fluidic control techniques and isothermal amplification methods. here, we demonstrate a lab-on-paper for all-in-one molecular diagnostics of zika, dengue, and ... | 2020 | 32729520 |
| smart diagnostics devices through artificial intelligence and mechanobiological approaches. | the present work illustrates the promising intervention of smart diagnostics devices through artificial intelligence (ai) and mechanobiological approaches in health care practices. the artificial intelligence and mechanobiological approaches in diagnostics widen the scope for point of care techniques for the timely revealing of diseases by understanding the biomechanical properties of the tissue of interest. smart diagnostic device senses the physical parameters due to change in mechanical, biol ... | 2020 | 32728518 |
| zika virus infection in pregnancy and adverse fetal outcomes in são paulo state, brazil: a prospective cohort study. | robust epidemiological and biological evidence supports a causal link between prenatal zika virus (zikv) infection and congenital brain abnormalities including microcephaly. however, it remains uncertain if zikv infection in pregnancy also increases the risk for other adverse fetal and birth outcomes. in a prospective cohort study we investigated the influence of zikv on the prevalence of prematurity, low birth weight, small-for-gestational-age, and fetal death as well as microcephaly (i.e., ove ... | 2020 | 32728054 |
| multifunctional t cell response in convalescent patients two years after zikv infection. | zika is an important emerging infectious disease in which the role of t cells remains elusive. this study aimed to evaluate the phenotype of multifunctional t cells in individuals 2 yr after exposure to zika virus (zikv). we used a library of 671 synthetic peptides covering the whole polyprotein of zikv in pools corresponding to each viral protein (i.e., capsid, membrane precursor or prm, envelope, ns1 [nonstructural protein], ns2a + ns2b, ns3, ns4a + ns4b, and ns5) to stimulate pbmcs from indiv ... | 2020 | 32726884 |
| case report: multiorgan involvement with congenital zika syndrome. | we report the case of an infant born with congenital zika syndrome (czs). during the largest zika virus (zikv) outbreak in peru, the mother presented with fever and rash that were confirmed to be due to zikv by real-time pcr. the infant was born with severe microcephaly. imaging revealed corpus callosum dysgenesis, lissencephaly, ventriculomegaly, and calcifications. mild hypertrophic cardiomyopathy with diastolic dysfunction was reported in the echocardiogram. valgus deviation of the lower extr ... | 2020 | 32723426 |
| brain organoids: tiny mirrors of human neurodevelopment and neurological disorders. | unravelling the complexity of the human brain is a challenging task. nowadays, modern neurobiologists have developed 3d model systems called "brain organoids" to overcome the technical challenges in understanding human brain development and the limitations of animal models to study neurological diseases. certainly like most model systems in neuroscience, brain organoids too have limitations, as these minuscule brains lack the complex neuronal circuitry required to begin the operational tasks of ... | 2020 | 32723210 |
| autophagy-related neurotoxicity is mediated via ahr and car in mouse neurons exposed to dde. | dde (dichlorodiphenyldichloroethylene) is an environmental metabolite of the pesticide ddt, which is still present in the environment, and its insecticidal properties are used to fight malaria and the zika virus disease. we showed for the first time that the neurotoxic effects of dde involve autophagy, as demonstrated by elevated levels of becn1, map1lc3a/map1lc3a, map1lc3b, and nup62/nup62 and an increase in autophagosome formation. the suggestion that the aryl hydrocarbon receptor (ahr) and th ... | 2020 | 32721735 |
| antagonism of interferon signaling by fibroblast growth factors promotes viral replication. | fibroblast growth factors (fgfs) play key roles in the pathogenesis of different human diseases, but the cross-talk between fgfs and other cytokines remains largely unexplored. we identified an unexpected antagonistic effect of fgfs on the interferon (ifn) signaling pathway. genetic or pharmacological inhibition of fgf receptor signaling in keratinocytes promoted the expression of interferon-stimulated genes (isg) and proteins in vitro and in vivo. conversely, fgf7 or fgf10 treatment of keratino ... | 2020 | 32720440 |
| what's new in vaccine science. | today vaccines can provide immunity against and treatment of a growing number of diseases including noninfectious conditions. vaccine science continues to evolve newer and safer ways to deliver prevention and treatment of infectious and noninfectious diseases. this includes new adjuvants to enhance immunogenicity; delivery systems to reduce pain and improve acceptability; a wider range of uses including preventing emerging infectious diseases, such as zika virus and ebola, treatment of chronic d ... | 2020 | 32718447 |
| simultaneous diagnosis of dengue virus, chikungunya virus, and zika virus infection using a new point-of-care testing (poct) system based on the loop-mediated isothermal amplification (lamp) method. | dengue fever, chikungunya fever, and zika virus infection have similar symptoms and overlapping areas of endemicity and so cannot be distinguished clinically. rapid diagnosis tests are available for each infection but each test covers only single target. there are pcr-based methods available that test for all three infections simultaneously, not applicable for in-vitro diagnostic use. the aim of this study was to evaluate the diagnostic accuracy of a new point-of-care testing (lamp poct) based o ... | 2020 | 32713745 |
| identification of lysinibacillus sphaericus binary toxin binding proteins in a malarial mosquito cell line by proteomics: a novel approach towards improving mosquito control. | bacterial insecticidal proteins, such as the bin toxin from lysinibacillus sphaericus, could be used more extensively to control insecticide resistant mosquitoes. this study was aimed at identification of mosquito cell proteins binding bin toxin. results showed that purified toxin was toxic to anopheles gambiae larvae and ag55 cultured cells. clathrin heavy chain (an endocytosis protein) and glycolytic enzymes such as pyruvate kinase, enolase and dihydrolipoamide dehydrogenase were identified as ... | 2020 | 32712372 |
| immunopathology of zika virus infection. | zika virus (zikv) is a mosquito-borne virus of the flavivirus genus in the flaviviridae family. flaviviruses are single-stranded, positive-sense rna viruses that have been responsible for numerous human epidemics. notable flaviviruses include mosquito-borne viruses such as yellow fever virus (yfv), dengue virus (denv), west nile virus (wnv), japanese encephalitis virus (jev), as well as tick-borne viruses including powassan virus (powv) and tick-borne encephalitis virus (tbev). despite having be ... | 2020 | 32711730 |
| zikv infection induces dna damage response and alters the proteome of gastrointestinal cells. | the zika virus (zikv) is a neurotropic virus that causes congenital abnormalities in babies when they are infected in utero. some studies have reported these congenital abnormalities result from zikv attacking neural progenitor cells within the brain which differentiate into neurons, oligodendrocytes, and astrocytes. each of these glial cells play important roles during development of the fetal brain. in addition to zikv-induced congenital abnormalities, infected patients experience gastrointest ... | 2020 | 32708879 |
| vector competence of aedes aegypti, aedes albopictus and culex quinquefasciatus from brazil and new caledonia for three zika virus lineages. | zika virus (zikv) has caused severe epidemics in south america beginning in 2015, following its spread through the pacific. we comparatively assessed the vector competence of ten populations of aedesaegypti and ae. albopictus from brazil and two of ae.aegypti and one of culex quinquefasciatus from new caledonia to transmit three zikv isolates belonging to african, asian and american lineages. recently colonized mosquitoes from eight distinct sites from both countries were orally challenged with ... | 2020 | 32708536 |
| high-quality resolution of the outbreak-related zika virus genome and discovery of new viruses using ion torrent-based metatranscriptomics. | arboviruses, including the zika virus, have recently emerged as one of the most important threats to human health. the use of metagenomics-based approaches has already proven valuable to aid surveillance of arboviral infections, and the ability to reconstruct complete viral genomes from metatranscriptomics data is key to the development of new control strategies for these diseases. herein, we used rna-based metatranscriptomics associated with ion torrent deep sequencing to allow for the high-qua ... | 2020 | 32708079 |
| congenital infections in hong kong: beyond torch. | congenital infections refer to a group of perinatal infections that are caused by pathogens transmitted from mother to child during pregnancy (transplacentally) or delivery (peripartum) which may have similar clinical presentations, including rash and ocular findings. torch is the acronym that covers these infections (toxoplasmosis, other [syphilis], rubella, cytomegalovirus, herpes simplex virus). other important causes of intrauterine/perinatal infection include human immunodeficiency virus, v ... | 2020 | 32705996 |
| polysaccharides like pentagalloylglucose, parishin a and stevioside inhibits the viral entry by binding the zika virus envelope protein. | zikv belongs to a flavivirus family in which class ii fusion proteins involve a low ph-dependent membrane fusion leading to infection of host cells. envelope (e) protein is primarily responsible for the viral host membrane fusion and is the major target for inhibiting viral entry. our findings reveal that compounds like pgg, parishin a, and stevioside have shown a high affinity for e protein and found to be active against various other viral infections. the binding of these molecules to e protei ... | 2020 | 32705969 |
| modeling and dynamics analysis of zika transmission with limited medical resources. | zika virus, a reemerging mosquito-borne flavivirus, posed a global public health emergency in 2016. brazil is the most seriously affected country. some measures have been implemented to control the zika transmission, such as spraying mosquitoes, developing vaccines and drugs. however, because of the limited medical resources (lmrs) in the country, not every infected patient can be treated in time when infected with zika virus. we aim to build a deterministic zika model by introducing a piecewise ... | 2020 | 32705573 |
| ns5 sumoylation directs nuclear responses that permit zika virus to persistently infect human brain microvascular endothelial cells. | zika virus (zikv) is cytopathic to neurons and persistently infects brain microvascular endothelial cells (hbmecs), which normally restrict viral access to neurons. despite replicating in the cytoplasm, zikv and dengue virus (denv) polymerases, ns5 proteins, are predominantly trafficked to the nucleus. we found that a sumo interaction motif in zikv and denv ns5 proteins directs nuclear localization. however, zikv ns5 formed discrete punctate nuclear bodies (nbs), while denv ns5 was uniformly dis ... | 2020 | 32699085 |
| intramuscular delivery of replicon rna encoding zikv-117 human monoclonal antibody protects against zika virus infection. | monoclonal antibody (mab) therapeutics are an effective modality for the treatment of infectious, autoimmune, and cancer-related diseases. however, the discovery, development, and manufacturing processes are complex, resource-consuming activities that preclude the rapid deployment of mabs in outbreaks of emerging infectious diseases. given recent advances in nucleic acid delivery technology, it is now possible to deliver exogenous mrna encoding mabs for in situ expression following intravenous ( ... | 2020 | 32695842 |
| a global lipid map defines a network essential for zika virus replication. | zika virus (zikv), an arbovirus of global concern, remodels intracellular membranes to form replication sites. how zikv dysregulates lipid networks to allow this, and consequences for disease, is poorly understood. here, we perform comprehensive lipidomics to create a lipid network map during zikv infection. we find that zikv significantly alters host lipid composition, with the most striking changes seen within subclasses of sphingolipids. ectopic expression of zikv ns4b protein results in simi ... | 2020 | 32694525 |
| ahr is a zika virus host factor and a candidate target for antiviral therapy. | zika virus (zikv) is a flavivirus linked to multiple birth defects including microcephaly, known as congenital zikv syndrome. the identification of host factors involved in zikv replication may guide efficacious therapeutic interventions. in genome-wide transcriptional studies, we found that zikv infection triggers aryl hydrocarbon receptor (ahr) activation. specifically, zikv infection induces kynurenine (kyn) production, which activates ahr, limiting the production of type i interferons (ifn-i ... | 2020 | 32690969 |
| on the correct interpretation of similarity index in codon usage studies: comparison with four other metrics and implications for zika and west nile virus. | viruses can modify the usage of their synonymous codons to mimic that of their hosts: investigation of codon usage may therefore help increase our understanding of virus biology. the similarity index (sid or (d(a,b)) is a recent method for estimating the resemblance of the overall codon usage of the hosts to that of a certain virus. here we report that this index has been systematically misinterpreted in the literature. we first review its mathematical meaning to show that a high value of sid in ... | 2020 | 32687862 |
| cellular imprinting proteomics assay: a novel method for detection of neural and ocular disorders applied to congenital zika virus syndrome. | congenital zika syndrome was first described due to increased incidence of congenital abnormalities associated with zika virus (zikv) infection. since the eye develops as part of the embryo central nervous system (cns) structure, it becomes a specialized compartment able to display symptoms of neurodegenerative diseases and has been proposed as a noninvasive approach to the early diagnosis of neurological diseases. ocular lesions result from defects that occurred during embryogenesis and can bec ... | 2020 | 32686424 |
| carneic acids from an endophytic phomopsis sp. as dengue virus polymerase inhibitors. | thirteen carneic acids were isolated from the fungal endophyte phomopsis sp. snb-lap1-7-32. their structures were identified by mass spectrometry and extensive one- and two-dimensional nmr spectroscopy and through comparison with data reported in the literature. compounds 1-13 were investigated for their antipolymerase activities against denv polymerase and zika ns5. five of them exhibited significant inhibition of dengue polymerase with ic50 values in the 10 to 20 μm range without cytotoxicity. ... | 2020 | 32686414 |
| enzyme-linked immunosorbent assays using virus-like particles containing mutations of conserved residues on envelope protein can distinguish three flavivirus infections. | the recent outbreaks of zika virus (zikv) in flavivirus-endemic regions highlight the need for sensitive and specific serological tests. previously we and others reported key fusion loop (fl) residues and/or bc loop (bcl) residues on dengue virus (denv) envelope protein recognized by flavivirus cross-reactive human monoclonal antibodies and polyclonal sera. to improve zikv serodiagnosis, we employed wild type (wt) and fl or fl/bcl mutant virus-like particles (vlp) of zikv, denv1 and west nile vi ... | 2020 | 32684139 |
| movement disorders in children with congenital zika virus syndrome. | congenital zika virus syndrome (czvs) denotes the neurologic and developmental sequelae of congenital infection of the zika virus. while prior studies have detailed the associated clinical phenotypes, new findings continue to be identified. abnormal postures and movements have been previously described in children with czvs, but not in detail. | 2020 | 32682638 |
| hope and trust in times of zika: the views of caregivers and healthcare workers at the forefront of the epidemic in brazil. | this article investigates how hope and trust played out for two groups at the forefront of the zika epidemic: caregivers of children with congenital zika syndrome and healthcare workers. we conducted 76 in-depth interviews with members of both groups to examine hope and trust in clinical settings, as well as trust in public institutions, in the health system and in the government of brazil. during and after the zika epidemic, hope and trust were important to manage uncertainty and risk, given th ... | 2020 | 32681164 |
| evolving viral and serological stages of zika virus rna-positive blood donors and estimation of incidence of infection during the 2016 puerto rican zika epidemic: an observational cohort study. | puerto rico began screening blood donations for zika virus rna with nucleic acid amplification tests (naats) on april 3, 2016, because of an emerging zika virus outbreak. we followed up positive donors to assess the dynamics of viral and serological markers during the early stages of zika virus infection and update the estimate of infection incidence in the puerto rican population during the outbreak. | 2020 | 32673594 |
| zika virus rna and igm persistence in blood compartments and body fluids: a prospective observational study. | characterisation of the dynamics of zika virus persistence following acute infection is needed to inform blood donor and diagnostic testing policies and understand the natural history of zika virus infection. we aimed to characterise the natural history, persistence, and clinical outcomes of zika virus infection through a prospective study in initially asymptomatic zika virus rna-positive blood donors. | 2020 | 32673593 |