| detection of aeromonas hydrophila in liquid media by volatile production similarity patterns, using a ff-2a electronic nose. | a technique for rapid detection of pathogenic microorganisms is essential for the diagnosis of associated infections and for food safety analysis. aeromonas hydrophila is one such food contaminant. several methods for rapid detection of this pathogen have been developed; these include multiplex polymerase chain reaction assays and the colony overlay procedure for peptidases. however, these conventional methods can only be used to detect the microorganisms at high accuracy after symptomatic onset ... | 2013 | 23296330 |
| burden of aeromonas hydrophila-associated diarrhea among children younger than 2 years in rural egyptian community. | between 2004 and 2007, a birth cohort of egyptian children was analysed to evaluate the epidemiology of enteric diseases. | 2012 | 23276737 |
| evaluation of probiotic bacteria against aeromonads syndrome in common carp (cyprinus carpio l.) in simple axenic larviculture. | evaluation of probiotics, bacillus firmus and b. coagulans against aeromonas hydrophila in axenic common carp larviculture was conducted. the highest egg hatching rate was obtained from the axenic system + probiotic bacteria (ap) (98.33%), followed by axenic system (a) (96.67%); axenic system + probiotic + a. hydrophila (ac) (93.33%); non-axenic system (na) (93.33%); and axenic system + a. hydrophila (ah) (83.33%). 100% survival rate (sr) was obtained from all treatments, except ah (90%). the hi ... | 2013 | 25141734 |
| effect of orally administered azadirachtin on non-specific immune parameters of goldfish carassius auratus (linn. 1758) and resistance against aeromonas hydrophila. | modulation of the immune responses using active bio-ingredients as a possible prophylaxis measure has been novel prospect for aquaculture. the present study evaluated the effects of azadirachtin ec 25% on non-specific immune responses in goldfish carassius auratus and resistance against pathogenic bacteria aeromonas hydrophila. the experimental trial for effects of azadirachtin on immuno-haematoloical parameters in goldfish was conducted by feeding the various levels of azadirachtin as control t ... | 2013 | 23261511 |
| an upstream initiator caspase 10 of snakehead murrel channa striatus, containing ded, p20 and p10 subunits: molecular cloning, gene expression and proteolytic activity. | caspase 10 (cscasp10) was identified from a constructed cdna library of freshwater murrel (otherwise called snakehead) channa striatus. the cscasp10 is 1838 base pairs (bp) in length and it is encoding 549 amino acid (aa) residues. cscasp10 amino acid contains two death effector domains (ded) in the n-terminal at 2-77 and 87-154 and it contains caspase family p20 domain (large subunit) and caspase family p10 domain (small subunit) in the c-terminal at 299-425 and 449-536 respectively. pairwise a ... | 2013 | 23253492 |
| analysis of a ferric uptake regulator (fur) knockout mutant in aeromonas salmonicida subsp. salmonicida. | aeromonas salmonicida subsp. salmonicida is the etiological agent of furunculosis; a serious infectious disease in aquaculture raised salmonids. iron acquisition has been shown to be critical for the survival of pathogenic bacteria during the course of infection. previous work has demonstrated that a. salmonicida expresses iron-repressible iromp proteins, suggesting the presence of iron acquisition systems that are under the control of a ferric uptake regulator (fur). in this study, the a. salmo ... | 2013 | 23228622 |
| evasion of mucosal defenses during aeromonas hydrophila infection of channel catfish (ictalurus punctatus) skin. | the mucosal surfaces of fish serve as the first line of defense against the myriad of aquatic pathogens present in the aquatic environment. the immune repertoire functioning at these interfaces is still poorly understood. the skin, in particular, must process signals from several fronts, sensing and integrating environmental, nutritional, social, and health cues. pathogen invasion can disrupt this delicate homeostasis with profound impacts on signaling throughout the organism. here, we investiga ... | 2013 | 23219904 |
| fermentation preparation of recombinant vibrio anguillarum vaccine with heterogeneous antigen display. | in the design of recombinant bacterial vector vaccine, heterogeneous antigen is displayed on the outer membrane of the vector strain to evoke polyvalent immunological protection. thus, the expression of heterogeneous antigen in cells and its display on the outer membrane are of great concern for vaccine preparation. in our previous work, a multivalent bacterial vector vaccine mvav6203a-1 was constructed by displaying the protective antigen gapdh from aeromonas hydrophila on the surface of an att ... | 2013 | 23215656 |
| acute diarrhea among military recruits. | there is a considerable amount of speculations concerning the presence of symptoms mainly diarrhea in various bacterial and parasitic infections. among a youth group with acute diarrhea, many bacterial and parasitic diseases were detected. the bacterial causes were campylobacter jejuni, shigella sp. entero-toxigenic, escherichia coli, salmonella sp. and aeromonas hydrophila. the parasitic causes were cryptosporidium parvum, entamoeba histolytica, giardia lamblia and strongyloides stercoralis. ma ... | 2012 | 23214210 |
| a case of extrusion of a solid silicone tire migrating through the superior rectus muscle with aeromonas hydrophila infection following a scleral buckling procedure. | to our knowledge, there are no reports of aeromonas hydrophila infection after a scleral buckling procedure. also, migration of a silicone explant element through the rectus muscles is extremely rare. herein, we describe a case experiencing extrusion of a solid silicone tire migrating through the superior rectus muscle with aeromonas hydrophila infection following a scleral buckling procedure. a 42-year-old man was referred to our hospital complaining of ocular pain and purulent discharge in his ... | 2012 | 23213585 |
| [identification of genomic structure and resistance trait associated snp loci in glutathione peroxidase gene of hyriopsis cumingii]. | based on the cdna sequence of gpx in hyriopsis cumingii, the complete genomic dna of gpx gene and it's 5'-flanking region were identified from h. cumingii using pcr and genome walking technique. the length of the complete genomic sequence was 6 708 bp including the 5'-flanking region, two exons, and one intron. sequence analysis of the 992 bp 5'-flanking region revealed that it contained a core promoter element (tata-box) and other transcription regulation elements such as ap1, c/ebp, and cdxa. ... | 2012 | 23208143 |
| effects of dietary cholesterol on antioxidant capacity, non-specific immune response, and resistance to aeromonas hydrophila in rainbow trout (oncorhynchus mykiss) fed soybean meal-based diets. | this study evaluated the effects of dietary cholesterol on antioxidant capacity, non-specific immune response and resistance to aeromonas hydrophila in rainbow trout (oncorhynchus mykiss) fed soybean meal-based diets. fish were fed diets supplemented with graded cholesterol levels (0 [control], 0.3, 0.6, 0.9, 1.2, and 1.5%) for nine weeks. the fish were then challenged by a. hydrophila and their survival rate recorded for the next week. dietary cholesterol supplementation generally increased the ... | 2013 | 23207478 |
| molecular characterization and immunological response analysis of a novel transferrin-like, pacifastin heavy chain protein in giant freshwater prawn, macrobrachium rosenbergii (de man, 1879). | the full-length cdna of the pacifastin heavy chain gene from giant freshwater prawn (macrobrachium rosenbergii, mr-phc) was cloned and characterized. the full sequence of the mr-phc cdna was 4331 bp and contained a 119-bp 5'-untranslated region (utr), a 3990-bp open reading frame (orf) encoding 1329 amino acid residues and a 222-bp 3' utr. the mr-phc protein predicted by its full orf, exhibited a unique transferrin-like protein structure containing 4 different lobes that have not been previously ... | 2012 | 23198290 |
| thin-film fixed-bed reactor for solar photocatalytic inactivation of aeromonas hydrophila: influence of water quality. | controlling fish disease is one of the major concerns in contemporary aquaculture. the use of antibiotics or chemical disinfection cannot provide a healthy aquaculture system without residual effects. water quality is also important in determining the success or failure of fish production. several solar photocatalytic reactors have been used to treat drinking water or waste water without leaving chemical residues. this study has investigated the impact of several key aspects of water quality on ... | 2012 | 23194331 |
| mtr extracellular electron-transfer pathways in fe(iii)-reducing or fe(ii)-oxidizing bacteria: a genomic perspective. | originally discovered in the dissimilatory metal-reducing bacterium shewanella oneidensis mr-1 (mr-1), key components of the mtr (i.e. metal-reducing) pathway exist in all strains of metal-reducing shewanella characterized. the protein components identified to date for the mtr pathway of mr-1 include four multihaem c-cyts (c-type cytochromes), cyma, mtra, mtrc and omca, and a porin-like outer membrane protein mtrb. they are strategically positioned along the width of the mr-1 cell envelope to me ... | 2012 | 23176465 |
| tetrahymena: an alternative model host for evaluating virulence of aeromonas strains. | an easier assessment model would be helpful for high-throughput screening of aeromonas virulence. the previous study indicated the potential of tetrahymena as a permissive model to examine virulence of aeromonas hydrophila. here our aim was to assess virulence of aeromonas spp. using two model hosts, a zebrafish assay and tetrahymena-aeromonas co-culture, and to examine whether data from the tetrahymena thermophila model reflects infections in the well-established animal model. first, virulence ... | 2012 | 23145022 |
| reply: is it really aeromonas hydrophila? | | 2013 | 23135799 |
| is it really aeromonas hydrophila? | | 2013 | 23135798 |
| a novel effect of imidazole derivative kk-42 on increasing survival of aeromonas hydrophila challenged prawn macrobrachium nipponense. | imidazole derivative kk-42 is well known as the insect growth regulator. here we find that kk-42 pretreatment could promote the survival of macrobrachium nipponense infected with aeromonas hydrophila, which is considered to be possibly related to the prophenoloxidase (propo), a conserved copper-containing enzyme that plays an important role in defense against pathogens. in this study, a full-length of propo gene from m. nipponense haemocytes, designated as mnpropo, was firstly cloned and charact ... | 2013 | 23123639 |
| antibiofilm activity of dendrophthoe falcata against different bacterial pathogens. | dendrophthoe falcata is a hemiparasitic plant commonly used for ailments such as ulcers, asthma, impotence, paralysis, skin diseases, menstrual troubles, pulmonary tuberculosis, and wounds. in this context, the validations of the traditional claim that the leaf extract of d. falcata possesses antibiofilm and anti-quorum sensing activity against different bacterial pathogens were assessed. the bacterial biofilms were quantified by crystal violet staining. among the 17 bacterial pathogens screened ... | 2012 | 23115018 |
| synthesis and anti-microbial potencies of 1-(2-hydroxyethyl)-3-alkylimidazolium chloride ionic liquids: microbial viabilities at different ionic liquids concentrations. | three 1-(2-hydroxyethyl)-3-alkylimidazolium chloride room temperature ionic liquids (ils) [2ohimc(n)][cl]; (n=0, 1, 4) have been synthesized from the appropriate imidazole precursors and characterized by ir and nmr spectroscopies and elemental analysis. their anti-microbial activities were investigated using the well-diffusion method. the viabilities of escherichia coli, aeromonas hydrophila, listeria monocytogenes and salmonella enterica as a function of il concentrations were studied. the mini ... | 2013 | 23107478 |
| infections following the application of leeches: two case reports and review of the literature. | | 2012 | 23098279 |
| enhanced biotransformation of ddts by an iron- and humic-reducing bacteria aeromonas hydrophila hs01 upon addition of goethite and anthraquinone-2,6-disulphonic disodium salt (aqds). | a fermentative facultative anaerobe, strain hs01 isolated from subterranean sediment, was identified as aeromonas hydrophila by 16s rrna sequence analysis. the biotransformation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (ddt), 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (ddd), and 1,1-dichloro-2,2-bis (4-chlorophenyl) ethane (dde) by hs01 was investigated in the presence of goethite and anthraquinone-2,6-disulphonic disodium salt (aqds). the results demonstrated that hs01 was capable o ... | 2012 | 23095105 |
| effects of enteromyxum leei (myxozoa) infection on gilthead sea bream (sparus aurata) (teleostei) intestinal mucus: glycoprotein profile and bacterial adhesion. | the intestinal myxosporean parasite enteromyxum leei causes severe desquamative enteritis in gilthead sea bream (sparus aurata) (teleostei) that impairs nutrient absorption causing anorexia and cachexia. in fish, as in terrestrial vertebrates, intestinal goblet cells are responsible for the adherent mucus secretion overlying epithelial cells, which constitutes a first line of innate immune defense against offending microorganisms but serves also as substrate and nutrient source for the commensal ... | 2013 | 23086443 |
| ciprofloxacin-resistant aeromonas hydrophila infection following leech therapy: a case report and review of the literature. | compromised flap perfusion can lead to the use of leech therapy to aid in flap salvage. aeromonas hydrophila, a symbiont of the leech, is a well-known bacterial pathogen that has the potential to cause infection in patients receiving leech therapy. ciprofloxacin is commonly regarded as the antibiotic of choice for prophylaxis against this pathogen. we present patient who underwent leech therapy during salvage attempts for a venous congested flap. a resultant infection developed despite being on ... | 2013 | 23084650 |
| immune response of turbot (scophthalmus maximus l.) to a broad spectrum vaccine candidate, recombinant glyceraldehyde-3-phosphate dehydrogenase of edwardsiella tarda. | edwardsiella tarda is a gram-negative pathogen which causes systemic infection in turbot. the increasing frequency of edwardsiellosis in turbot farming has stressed the need to understand the immune responses of fish, for the further development of prevention and control strategies. as a broad spectrum protective antigen, a recombinant glyceraldehyde-3-phosphate dehydrogenase (gapdh) of e. tarda eib202 has been proven to present remarkable protection against e. tarda, aeromonas hydrophila, vibri ... | 2012 | 23084627 |
| crustin, a wap domain containing antimicrobial peptide from freshwater prawn macrobrachium rosenbergii: immune characterization. | crustin (mrcrs) was sequenced from a freshwater prawn macrobrachium rosenbergii. the mrcrs protein contains a signal peptide region at n-terminus between 1 and 22 and a long whey acidic protein domain (wap domain) at c-terminus between 57 and 110 along with a wap-type 'four-disulfide core' motif. phylogenetic results show that mrcrs is clustered together with other crustacean crustin groups. mrcrs showed high sequence similarity (77%) with crustin from pacific white shrimp litopenaeus vannamei a ... | 2013 | 23069787 |
| identification of omp38 by immunoproteomic analysis and evaluation as a potential vaccine antigen against aeromonas hydrophila in chinese breams. | aeromonas hydrophila is a fish pathogen causing systemic infections in aquatic environments, and determining its antigenic proteins is important for vaccine development to reduce economic losses in aquaculture worldwide. here, an immunoproteomic approach was used to identify immunogenic outer membrane proteins (omps) of the chinese vaccine strain j-1 using convalescent sera from chinese breams. seven unique immunogenic proteins were identified by two-dimensional (2-d) electrophoresis and matrix- ... | 2013 | 23063539 |
| cutaneous abscess leading to sepsis by aeromonas hydrophila. | | 2013 | 23055148 |
| molecular cloning, characterization and mrna expression of copper-binding protein hemocyanin subunit in chinese mitten crab, eriocheir sinensis. | hemocyanin is a copper-binding protein and plays a crucial role in the physiological processes in crustacean. in this study, the cdna encoding hemocyanin subunit from chinese mitten crab eriocheir sinensis (eshc) was cloned by using est analysis and rapid amplification of cdna ends (race) approach. the full-length cdna of eshc was 2573 bp, consisting of a 5' untranslated region of 51 bp, a 3' untranslated region of 458 bp, and an open reading frame of 2064 bp. the deduced protein had 688 amino a ... | 2012 | 23032440 |
| identification of bacillus strains for biological control of catfish pathogens. | bacillus strains isolated from soil or channel catfish intestine were screened for their antagonism against edwardsiella ictaluri and aeromonas hydrophila, the causative agents of enteric septicemia of catfish (esc) and motile aeromonad septicaemia (mas), respectively. twenty one strains were selected and their antagonistic activity against other aquatic pathogens was also tested. each of the top 21 strains expressed antagonistic activity against multiple aquatic bacterial pathogens including ed ... | 2012 | 23029244 |
| effect of dietary supplementation with achyranthes aspera seed on larval rohu labeo rohita challenged with aeromonas hydrophila. | larval rohu labeo rohita were fed four different diets: three of the diets contained achyranthes aspera (prickly chaff-flower) seeds at 0.10% (d1), 0.25% (d2), or 0.50% (d3); the fourth diet was a control diet (d4; no a. aspera supplementation). after 70 d, the rohu were injected intraperitoneally with live aeromonas hydrophila. mortality of fish was recorded for 7 d. in the d4 group, the first mortality was observed within 12 h of exposure, whereas in the d1-d3 treatment groups, mortality was f ... | 2012 | 23025591 |
| a cytotoxicity and comparative antibacterial study on the effect of zataria multiflora boiss, trachyspermum copticum essential oils, and enrofloxacin on aeromonas hydrophila. | this study designed to test the antibacterial potency of enrofloxacin (enr) and essential oils from zataria multiflora boiss (zeo) and trachyspermum copticum (teo) on aeromonas hydrophila. | 2012 | 25050249 |
| extractive biodecolorization of triphenylmethane dyes in cloud point system by aeromonas hydrophila dn322p. | the biological treatment of triphenylmethane dyes is an important issue. most microbes have limited practical application because they cannot completely detoxicate these dyes. in this study, the extractive biodecolorization of triphenylmethane dyes by aeromonas hydrophila dn322p was carried out by introducing the cloud point system. the cloud point system is composed of a mixture of nonionic surfactants (20 g/l) brij 30 and tergitol tmn-3 in equal proportions. after the decolorization of crystal ... | 2013 | 23008002 |
| inhibition of a cold-active alkaline phosphatase by imipenem revealed by in silico modeling of metallo-β-lactamase active sites. | we demonstrate the inhibition of the native phosphatase activity of a cold active alkaline phosphatase from vibrio (vap) (ic(50) of 44±4 (n=4)μm at ph 7.0 after a 30min preincubation) by a specific β-lactam compound (only by imipenem, and not by ertapenem, meropenem, ampicillin or penicillin g). the homologous scaffold was detected by an in silico analysis that established the spatial and electrostatic congruence of the active site of a class b2 cpha metallo-β-lactamase from aeromonas hydrophila ... | 2012 | 22982109 |
| characterization of an actin-targeting adp-ribosyltransferase from aeromonas hydrophila. | the mono-adp-ribosyltransferase (mart) toxins are contributing factors to a number of human diseases, including cholera, diphtheria, traveler's diarrhea, and whooping cough. vahc is a cytotoxic, actin-targeting mart from aeromonas hydrophila ppd134/91. this bacterium is implicated primarily in diseases among freshwater fish species but also contributes to gastrointestinal and extraintestinal infections in humans. vahc was shown to adp-ribosylate arg-177 of actin, and the kinetic parameters were ... | 2012 | 22969084 |
| complete genome sequence of aeromonas hydrophila phage cc2. | aeromonas hydrophila is one of the major pathogenic bacteria for fish and people. to develop an effective antimicrobial agent, we isolated a bacteriophage from sewage, named cc2, and sequenced its genome. comparative genome analysis of phage cc2 with its relatives revealed that phage cc2 has higher sequence homology to a. salmonicida phage 65 than to a. hydrophila phage aeh1. here, we announce the complete genome sequence of cc2 and report major findings from the genomic analysis. | 2012 | 22966192 |
| opportunistic pathogens and faecal indicators in drinking water associated biofilms in cluj, romania. | biofouling occurs without exception in all water systems, with undesirable effects such as biocorrosion and deterioration of water quality. drinking water associated biofilms represent a potential risk to human health by harbouring pathogenic or toxin-releasing microorganisms. this is the first study investigating the attached microbiota, with potential threat to human health, in a public water system in romania. the presence and the seasonal variation of viable faecal indicators and opportunist ... | 2012 | 22960491 |
| β-glucan protects neutrophil extracellular traps against degradation by aeromonas hydrophila in carp (cyprinus carpio). | a novel host innate immune defence mechanism against invading pathogens, namely the formation of neutrophil extracellular traps (nets), has recently been discovered. these nets are described as dna fibres released by dying neutrophils, which are able to entrap and kill various microbes. here we studied the effect of the feed additive β-glucan, namely macrogard(®), on the degradation of nets by the important fish pathogen aeromonas hydrophila. therefore, common carp (cyprinus carpio) head kidney ... | 2012 | 22959188 |
| construction, expression and characterization of 11 putative flagellar apparatus genes of aeromonas hydrophila al09-73. | | 2012 | 22924657 |
| evaluation of two outer membrane proteins, aha1 and ompw of aeromonas hydrophila as vaccine candidate for common carp. | aeromonas hydrophila is an important fish pathogen responsible for huge economic losses in aquaculture sector. the bacterial outer membrane proteins (omps), especially adhesins play a key role in the virulence of the bacteria and are considered potential vaccine candidates. we evaluated the immunogenicity of two important outer membrane proteins namely aha1 and ompw of a. hydrophila. these proteins were over-expressed in escherichia coli, purified and used for the vaccination of common carp. seq ... | 2012 | 22917476 |
| attenuation of a virulent aeromonas hydrophila with novobiocin and pathogenic characterization of the novobiocin-resistant strain. | to determine whether novobiocin resistance strategy could be used to attenuate a virulent aeromonas hydrophila ah11p strain and to characterize the growth and pathogenic differences between the novobiocin-resistant strain and its virulent parent strain ah11p. | 2012 | 22897434 |
| antimicrobial activity of bioactive compounds and leaf extracts in jatropha tanjorensis. | jatropha tanjorensis was investigated scientifically to generate evidence for the efficacies reported in traditional systems and the results are given here. | 2012 | 22884742 |
| development of an aeromonas hydrophila recombinant extracellular protease vaccine. | aeromonas hydrophila (ah) exists widely in the aquatic environment and infects a variety of animals. extracellular protease (epr) is an important protective antigen that induces a specific antibody response to resist ah infection. in this study, two genes encoding extracellular protease epr2 and epr3 were linked within the expression vector pet32a to construct a recombinant pet-epr2-3 plasmid. the immunogenicity of the fusion protein epr2-3 was investigated as a subunit vaccine in icr mice. the ... | 2012 | 22874879 |
| first description of cole-type plasmid in aeromonas spp. carrying quinolone resistance (qnrs2) gene. | isolation and full sequence analysis of cole-type plasmid, which carries the qnrs2 gene. | 2012 | 22862417 |
| the influences of natural zeolite (cliptinolite) on ammonia and biogenic amine formation by foodborne pathogen. | the influence of natural zeolite on biogenic amines (bas) and ammonia (amn) production by eight common gram negative and positive foodborne pathogens (fbp) were investigated in histidine decarboxylase broth (hdb). presence of 1% zeolite in the hdb resulted in significantly higher amn production. histamine (his) production by gram positive bacteria was as low as 0.5 mg/l, whereas escherichia coli produced 18.96 mg/l of his. the use of zeolite also significantly suppressed his accumulation by e. c ... | 2012 | 22860594 |
| effect of high hydrostatic pressure on aeromonas hydrophila ah 191 growth in milk. | exposure to high pressure is an efficient method of bacterial inactivation that is particularly important for reducing the microbial load present in foods. in this study, we examined the high pressure inactivation of aeromonas hydrophila ah 191, a virulent strain that produces aerolysin, a cytotoxic, enterotoxic, and hemolytic toxin. high pressure treatment (250 mpa for 30 min at 25 °c in 0.1 m pbs, ph 7.4) of a. hydrophila grown in milk reduced bacterial viability by at least 9 orders of magnit ... | 2012 | 22860590 |
| antimicrobial-immunomodulatory activities of zebrafish phosvitin-derived peptide pt5. | a phosvitin (pv)-derived peptide, pt5, which consists of the c-terminal 55 residues of pv in zebrafish, has been shown to function as an antimicrobial agent capable of killing microbes in vitro. however, its in vivo role in zebrafish remains unknown. in this study, we clearly demonstrated that pt5 protected adult zebrafish from pathogenic aeromonas hydrophila attack, capable of significantly enhancing the survival rate of zebrafish after the pathogenic challenge. pt5 also caused a marked decreas ... | 2012 | 22841856 |
| antimicrobial assays of natural extracts and their inhibitory effect against listeria innocua and fish spoilage bacteria, after incorporation into biopolymer edible films. | the antimicrobial activity of twelve natural extracts was tested against two fish spoilage bacteria (pseudomonas fluorescens and aeromonas hydrophila/caviae) and listeria innocua, in order to assess their potential utilization in the preservation and safety of minimally processed fish products. after a screening of the active extracts by agar diffusion and vapour diffusion methods, oregano and thyme essential oils and citrus extract were selected. the minimum inhibitory concentration (mic) of th ... | 2012 | 22824340 |
| changes in adhesion ability of aeromonas hydrophila during long exposure to salt stress conditions. | stressful environmental conditions influence both bacterial growth and expression of virulence factors. in the present study, we evaluated the influence of nacl on aeromonas hydrophila adhesiveness at two temperatures. this agent is often involved in clinical cases; however, its pathogenic potential is still not fully understood. | 2012 | 22805151 |
| assessment of aquaflor(®) , copper sulphate and potassium permanganate for control of aeromonas hydrophila and flavobacterium columnare infection in sunshine bass, morone chrysops female × morone saxatilis male. | two experiments were conducted to test the effectiveness of different therapeutants against a mixed infection of aeromonas hydrophila and flavobacterium columnare in sunshine bass. experiment 1 evaluated copper sulphate, florfenicol-medicated feed and potassium permanganate (kmno(4) ) against a natural mixed infection. experiment 2 further evaluated copper sulphate as a treatment to control an experimental mixed infection. in experiment 1, naturally infected untreated fish had the lowest final s ... | 2012 | 22804750 |
| expression profiles of nods in channel catfish (ictalurus punctatus) after infection with edwardsiella tarda, aeromonas hydrophila, streptococcus iniae and channel catfish hemorrhage reovirus. | nlrs are a large family of intracellular pathogen recognition receptors (prrs) that recognize pathogen associated molecular patterns (pamps). in the previous study, the identification of nlrs subfamily a (nods) and gene expression was carried out in channel catfish (ictalurus punctatus). however, the gene expression profiles of channel catfish nods (nod1, nod2, nlrc3, nlrc5 and nlrx1) after infection with various bacteria and virus are still unclear. in this study, expression of five nods genes ... | 2012 | 22796486 |
| effect of coriolus versicolor polysaccharides on the hematological and biochemical parameters and protection against aeromonas hydrophila in allogynogenetic crucian carp (carassius auratus gibelio). | the effect of dietary intake of coriolus versicolor polysaccharides (cvp) on the hematological and biochemical indices of allogynogenetic crucian carp (carassius auratus gibelio) was investigated. fish were fed cvp supplemented diets (0, 0.25, 0.5, 1.0, 2.0 or 4.0 g cvp kg(-1)) for 56 days. the rbc, wbc counts, hemoglobin content, esr in blood and tp, alt, ast, alp, glu, cho, tg, and bun in serum were measured on day 0, 14, 28, 42, and 56. after feeding of 56 days, fish were infected with aeromo ... | 2013 | 22791194 |
| immunostimulatory response induced by supplementation of ficus benghalensis root powder, in the artificial feed the indian freshwater murrel, channa punctatus. | methanol extract from the dried aerial root of ficus benghalensis, was used to evaluate antibacterial activity on the bacterial strains of aeromonas hydrophila and escherichia coli, by disc diffusion method. in order to study, if there is any immunostimulatory response of f. benghalensis, immunized fish were fed with supplementary artificial feed containing 5% f. benghalensis dried root powder. there was no marked difference in the levels of alanine aminotransferase (alt) and aspartate aminotran ... | 2012 | 22789713 |
| purification and characterization of yfgap, a gapdh-related novel antimicrobial peptide, from the skin of yellowfin tuna, thunnus albacares. | a 3.4 kda of antimicrobial peptide was purified from an acidified skin extract of the yellowfin tuna, thunnus albacares, by preparative acid-urea-polyacrylamide gel electrophoresis and c(18) reversed-phase hplc. a comparison of the n-terminal amino acid sequence of the purified peptide with that of other known polypeptides revealed high homology with the n-terminus of glyceraldehyde-3-phosphate dehydrogenase (gapdh); thus, this peptide was designated as the yellowfin tuna gapdh-related antimicro ... | 2012 | 22771964 |
| histopathological biomarkers in pacu (piaractus mesopotamicus) infected with aeromonas hydrophila and treated with antibiotics. | pharmacochemicals usage in fish farming disease treatment can cause morphological and functional changes in absorption capacity, metabolism and excretion organs. the aim of this research was to evaluate the histopathological biomarkers in the gills, liver, kidney and skin of pacu (p. mesopotamicus), which have been infected with a. hydrophila and treated with oxytetracycline (otc) and florfenicol (ffc). fish were exposed to 2.4×10(7) ml(-1) of a. hydrophila bacteria experimental infection and af ... | 2012 | 22766414 |
| immune role of mrnfκbi-α, an iκb family member characterized in prawn m. rosenbergii. | nf kappa b inhibitor alpha (mrnfκbi-α) was sequenced from a freshwater prawn macrobrachium rosenbergii. the mrnfκbi-α protein contains a long ankyrin repeat region circular domain between 193 and 413 along with its 6 repeats (ankyrin repeat 1,2,3,4,5 and 6). an iκb degradation motif and a putative pest motif is present at 37-64 and 418-471 of the n- and c-terminal regions of mrnfκbi-α respectively. the gene expressions of mrnfκbi-α in healthy and infectious hematopoietic and hypodermal necrosis ... | 2012 | 22750025 |
| identity and virulence properties of aeromonas isolates from diseased fish, healthy controls and water environment in china. | to investigate the species distribution in aeromonas isolates from diseased fish, healthy controls and water environment in china; to evaluate the frequency of the aerolysin (aer), cytotonic enterotoxin (alt), cytotoxic enterotoxin (act), temperature-sensitive protease (eprcai) and serine protease (ahp) genes in aeromonas isolates; and to determine the potential pathogenicity of these isolates. | 2012 | 22725694 |
| expression pattern, promoter activity and bactericidal property of β-defensin from the mandarin fish siniperca chuatsi. | β-defensin (bd) are cysteine-rich, cationic antimicrobial peptides which play an important role in innate immune system against invading microbes. in the present study, the cdna cloning, expression analysis, transcriptional regulation and antimicrobial activity of β-defensin (scbd) from mandarin fish (siniperca chuatsi) were characterized. the cdna sequence of scbd is 596 bp which encodes a protein of 63 amino acids (aa). the scbd gene comprises three exons and two introns. the signal peptide is ... | 2012 | 22705342 |
| enterobacter asburiae and aeromonas hydrophila: soft tissue infection requiring debridement. | enterobacter asburiae and aeromonas hydrophila are gram-negative bacilli that have been isolated in soil and water. enterobacter asburiae can cause an array of diseases, and exposure to a hydrophila can cause soft tissue infections, including necrotizing faciitis.a healthy-appearing 22-year-old man presented with an innocuous soft tissue injury to his leg due to an all-terrain vehicle crash. he received intravenous antibiotics and was discharged with prophylactic oral antibiotics. after the rapi ... | 2012 | 22691684 |
| coenzyme depletion by members of the aerolysin family of pore-forming toxins leads to diminished atp levels and cell death. | recent studies demonstrated that a variety of bacterial pore-forming toxins induce cell death through a process of programmed necrosis characterized by the rapid depletion of cellular atp. however, events leading to the necrosis and depletion of atp are not thoroughly understood. we demonstrate that atp-depletion induced by two pore-forming toxins, the clostridium perfringens epsilon-toxin and the aeromonas hydrophila aerolysin toxin, is associated with decreased mitochondrial membrane potential ... | 2012 | 22688384 |
| a new molecular approach to help conclude drowning as a cause of death: simultaneous detection of eight bacterioplankton species using real-time pcr assays with taqman probes. | we developed a novel tool for concluding drowning as a cause of death. we designed nine primer pairs to detect representative freshwater or marine bacterioplankton (aquatic bacteria) and then used real-time pcr with taqman probes to rapidly and specifically detect them. we previously cultured the genus aeromonas, which is a representative freshwater bacterial species, in blood samples from 94% of victims who drowned in freshwater and the genera vibrio and/or photobacterium that are representativ ... | 2012 | 22682932 |
| recovery of aeromonas hydrophila associated with bacteraemia in captive snakes. | captive snakes, that is, a jamaican boa (epicrates subflavus) a yellow anaconda (eunectes notaeus) and a corn snake (pantherophis guttatus guttatus), died with signs of bacteraemia including the presence of petechial haemorrhages in the mouth and gums and haemorrhages in the lung, spleen and intestines. the abdomen and anus were swollen with bloody-tinged mucus in the colon. aeromonas hydrophila was recovered in dense virtually pure culture growth from the internal organs. characterization of th ... | 2012 | 22680932 |
| evaluation of the acetone and aqueous extracts of mature stem bark of sclerocarya birrea for antioxidant and antimicrobial properties. | we assayed the antimicrobial activity of acetone and aqueous extracts of the stem bark of sclerocarya birrea on some selected bacteria and fungi species including; streptococcus pyogenes, plesiomonas shigelloides, aeromonas hydrophila, salmonella typhimurium, cryptococcus neoformans, candida glabrata, trichosporon mucoides, and candida krusei using both agar well diffusion and minimum inhibitory concentration (mic) assays. based on the levels of activity, the acetone extract was examined for tot ... | 2012 | 22675390 |
| impact of qsebc system in c-di-gmp-dependent quorum sensing regulatory network in a clinical isolate ssu of aeromonas hydrophila. | our earlier studies showed that ahyri- (ai-1) and luxs-based (ai-2) quorum sensing (qs) systems were positive and negative regulators of virulence, respectively, in a diarrheal isolate ssu of aeromonas hydrophila. recently, we demonstrated that deletion of the qsebc two-component signal transduction system (ai-3 qs in enterohemorrhagic escherichia coli) also led to an attenuation of a. hydrophila in a septicemic mouse model of infection, and that interplay exists between ai-1, ai-2, and the seco ... | 2012 | 22664750 |
| screening of microbial contamination and antimicrobial activity of sea cucumber holothuria polii. | microbiological studies were carried out on microbial contamination and antimicrobial activity of sea cucumber holothuria polii collected from mediterranean sea at abu-kir shore of alexandria, egypt. the obtained results revealed the presence of isolates of five human gram-negative pathogenic bacteria, representing five genera were identified to species level, including, esherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella sp. and shigella sp. in addition, an yeast candida ... | 2013 | 22653870 |
| structural characterization of the plig lysozyme inhibitor family. | several gram-negative bacteria protect themselves against the lytic action of host lysozymes by producing specific proteinaceous inhibitors. so far, four different families of lysozyme inhibitors have been identified including ivy (inhibitor of vertebrate lysozyme), mlic/plic (membrane associated/periplasmic inhibitor of c-type lysozyme), plii and plig (periplasmic inhibitors of i- and g-type lysozymes, respectively). here we provide the first crystallographic description of the plig family. cry ... | 2012 | 22634186 |
| detection of toxigenic vibrio cholerae with new multiplex pcr. | vibrio cholerae (v. cholerae) is the etiological agent of epidemic cholera. in recent years, cholera has become endemic in different regions of the world. traditional culture and microscopic methods are considered the standard for the diagnosis of v. cholerae. however, these methods require days for confirmation. therefore, molecular methods that may be used for the sensitive, accurate, and rapid analysis of v. cholerae are urgently needed. | 2012 | 22632601 |
| matrix metalloproteinase 2 of grass carp ctenopharyngodon idella (cimmp2) is involved in the immune response against bacterial infection. | the gene encoding matrix metalloproteinase 2 (mmp2) was cloned from grass carp (ctenopharyngodon idella), and its expression levels during aeromonas hydrophila infection and embryonic development stages were evaluated. the complete open reading frame of cimmp2 was 1974 bp in length, encoding a 658-amino acid polypeptide. the deduced mmp2 protein contained four conserved domain structures, including an n-terminal signal sequence, a propeptide domain, three repeats of fibronectin-type ii domain in ... | 2012 | 22626808 |
| removal of triphenylmethane dyes by bacterial consortium. | a new consortium of four bacterial isolates (agrobacterium radiobacter; bacillus spp.; sphingomonas paucimobilis, and aeromonas hydrophila)-(cm-4) was used to degrade and to decolorize triphenylmethane dyes. all bacteria were isolated from activated sludge extracted from a wastewater treatment station of a dyeing industry plant. individual bacterial isolates exhibited a remarkable color-removal capability against crystal violet (50 mg/l) and malachite green (50 mg/l) dyes within 24 h. interestin ... | 2012 | 22623907 |
| dietary carbohydrate/lipid ratios affect stress, oxidative status and non-specific immune responses of fingerling blunt snout bream, megalobrama amblycephala. | this study aimed to evaluate the effects of dietary carbohydrate/lipid (cho:l) ratios on stress, liver oxidative status and non-specific immune responses of fingerling blunt snout bream. fish were fed six isonitrogenous and isoenergetic diets containing various cho:l ratios for 10 weeks. after the feeding trial, fish were challenged by aeromonas hydrophila and survival rate was recorded for the next 10 days. the lowest plasma cortisol, lactate, aspartate aminotransferase and alanine aminotransfe ... | 2012 | 22617792 |
| expression of complement component c7 and involvement in innate immune responses to bacteria in grass carp. | activation of the complement system, which forms a major part of the innate immune system, results in the formation of the terminal complement complex. the complement component, c7, plays an integral role in the assembly of this complex within target cell membranes. in this study, c7 was isolated and characterized from grass carp, an important cultured fish in china. the predicted amino acid sequence of c7 cdna (2644 bp) exhibited 55.4 and 48.3% homology with trout c7-1 and zebrafish c7, respect ... | 2012 | 22617254 |
| [transient expression of aeromonas hydrophila hbnuah01 outer membrane protein a gene in tobacco leaf cells]. | to clone outer membrane protein a (ompa) gene from aeromonas hydrophila hbnuah01 and transiently express this protein in tobacco (nicotiana tabacum) leaf cells. | 2012 | 22586996 |
| development of a multiplex polymerase chain reaction to detect five common gram-negative bacteria of aquatic animals. | a multiplex polymerase chain reaction (m-pcr) technique was developed as a rapid and accurate diagnostic tool for identifying five major gram-negative bacilli -vibrio vulnificus, v. parahaemolyticus, aeromonas hydrophila, chryseobacterium meningosepticum and edwardsiella tarda- that cause major diseases in cultured aquatic animals in taiwan. the expected amplicons for v. vulnificus, v. parahaemolyticus, a. hydrophila, c. meningosepticum and e. tarda were 410, 368, 685, 180 and 230bp, respectivel ... | 2012 | 22571515 |
| recombinant aeromonas hydrophila outer membrane protein 48 (omp48) induces a protective immune response against aeromonas hydrophila and edwardsiella tarda. | the gene coding for an outer membrane protein omp48 of aeromonas hydrophila isolated from an infected fish was cloned and sequenced. analysis of nucleotide sequence showed the omp48 gene to be an adhesin encoding a protein of 426 amino acids with high identity to the omp48 gene of aeromonas veronii, another fish pathogen. the gene belonged to the maltoporin group of porins and had high similarity to lamb porins of a. hydrophila, aeromonas salmonicida and vibrio parahaemolyticus. the expressed pu ... | 2012 | 22564558 |
| the effect of mistletoe, viscum album coloratum, extract on innate immune response of nile tilapia (oreochromis niloticus). | the purpose of the present study was to evaluate the effect of dietary mistletoe extracts on non-specific immune response and disease resistance of nile tilapia (oreochromis niloticus) against aeromonas hydrophila infection. tilapia fingerlings were fed with a diet containing 0 mg as a control, 10 mg, 50 mg, and 200 mg mistletoe powder kg(-1) dry diet for 80 days. the immunological parameters, respiratory burst activity, lysozyme activity, alternative complement haemolysis activity (ach(50)), an ... | 2012 | 22554573 |
| haemato-immunological and growth response of mirror carp (cyprinus carpio) fed a tropical earthworm meal in experimental diets. | an investigation was conducted to evaluate the effect of feeding a tropical earthworm meal (perionyx escavatus) on the haemato-immunological response and growth performance of mirror carp (cyprinus carpio). fish were fed diets for a total of 88 days, fishmeal served as the main protein source in the control diet. two remaining diets consisted of fishmeal fixed at 33.65% provision of protein and the remaining 66.35% protein was provided by soybean meal (sbm diet) or p. excavatus meal (ew diet). c ... | 2012 | 22554572 |
| maternal transfer and protective role of antibodies in zebrafish danio rerio. | maternal transfer of antibodies from mother to eggs has been reported in various species of fishes, and these antibodies have been proposed to play immune roles in developing embryos and larvae. however, firm evidence supporting this remains lacking. in this study, we clearly demonstrated that immunization of female zebrafish with the hapten-carrier complex, trinitrophenylated bovine serum albumin (tnp-bsa), induced a significant increase in anti-tnp antibody production in the mothers, which in ... | 2012 | 22551698 |
| purification and characterization of an antimicrobial histone h1-like protein and its gene from the testes of olive flounder, paralichthys olivaceus. | an approximately 21 kda antimicrobial protein was purified from an acidified testis extract of olive flounder, paralichthys olivaceus, by ion-exchange and c(18) reversed-phase hplc. a comparison of the n-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone h1 molecules; thus, it was designated flounder histone h1-like protein (fh1lp). fh1lp showed potent antimicrobial activity against gram-po ... | 2012 | 22538352 |
| molecular cloning and characterization of nucleotide binding and oligomerization domain-1 (nod1) receptor in the indian major carp, rohu (labeo rohita), and analysis of its inductive expression and down-stream signalling molecules following ligands exposure and gram-negative bacterial infections. | nucleotide binding and oligomerization domain-1 (nod1) is a cytoplasmic pattern recognition receptor (prr), and is a member of the nod-like receptor (nlr) family. it senses a wide range of bacteria and viruses or their products, and plays a key role in inducing innate immunity. in this report, nod1 gene was cloned and characterized in rohu (labeo rohita), a fish species of highest commercial importance in the indian subcontinent. the full-length rohu nod1 (rnod1) cdna comprised of 3168 bp with a ... | 2012 | 22530240 |
| the expression of prophenoloxidase mrna in red swamp crayfish, procambarus clarkii, when it was challenged. | the expression of the prophenoloxidase (propo) gene was investigated in nine tissues of red swamp crayfish procambarus clarkii, by real-time pcr after challenges by cpg oligodeoxynucleotide (odn), aeromonas hydrophila and white spot syndrome virus (wssv). the results can be summarized as follows: (i) the expression level of the propo gene in haemocytes was highest among nine studied tissues before the challenge; (ii) the expression of propo increased in all studied tissues after stimulation by c ... | 2012 | 22522035 |
| denaturing gradient gel electrophoresis for nonlethal detection of aeromonas salmonicida in salmonid mucus and its potential for other bacterial fish pathogens. | denaturing gradient gel electrophoresis (dgge) of 16s rdna was used to nonlethally detect aeromonas salmonicida and other bacteria in salmonid skin mucus. mucus samples from wild spawning coho salmon (oncorhynchus kisutch) with endemic a. salmonicida and from cultured lake trout (salvelinus namaycush) were tested by pcr-dgge and were compared with mucus culture on coomassie brilliant blue agar and internal organ culture. pcr-dgge gave a highly reproducible 4-band pattern for 9 strains of typical ... | 2012 | 22506865 |
| the impact of quorum sensing on the virulence of aeromonas hydrophila and aeromonas salmonicida towards burbot (lota lota l.) larvae. | in this study, the link between quorum sensing in aeromonas spp. and its virulence towards burbot (lota lota) was investigated. high mortality occurred in burbot juveniles challenged with aeromonas salmonicida hn-00, but not in juveniles challenged with aeromonas hydrophila ah-1n. meanwhile, both a. hydrophila ah-1n and a. salmonicida hn-00 were virulent towards larvae. the effect of quorum sensing on the virulence of a. hydrophila ah-1n towards burbot larvae was further investigated using quoru ... | 2012 | 22465799 |
| effects of dietary supplementation of potential probiotic pseudomonas aeruginosa vsg-2 on the innate immunity and disease resistance of tropical freshwater fish, labeo rohita. | the effects of dietary pseudomonas aeruginosa vsg-2 supplementation on innate immunity and protection against aeromonas hydrophila infection were evaluated in labeo rohita. fish were fed for 60 days with control diet or 3 experimental diets containing p. aeruginosa vsg-2 at 10(5), 10(7), and 10(9) cfu g(-l), respectively. various innate immune parameters were examined at 30 and 60 days post-feeding. fish were challenged with a. hydrophila 60 days post-feeding and mortalities were recorded over 1 ... | 2012 | 22465361 |
| light and transmission electron microscopy of vibrio campbellii infection in gnotobiotic artemia franciscana and protection offered by a yeast mutant with elevated cell wall glucan. | luminescent vibrios are amongst the most important pathogens in aquaculture, affecting almost all types of cultured organisms. vibrio campbellii is one of these most important pathogens. in this study, the effects of feeding mnn9 yeast cell wall mutant and wild type yeast strain were investigated in the digestive tract of brine shrimp nauplii, artemia franciscana, after experimental infection with v. campbellii (lmg 21363). gnotobiotic a. franciscana nauplii were fed daily with dead aeromonas hy ... | 2012 | 22464490 |
| impact of antibiotic treatments on the expression of the r plasmid tra genes and on the host innate immune activity during pras1 bearing aeromonas hydrophila infection in zebrafish (danio rerio). | the transfer of r plasmids between bacteria has been well studied under laboratory conditions and the transfer frequency has been found to vary between plasmids and under various physical conditions. for the first time, we here study the expression of the selected plasmid mobility genes trad, virb11 and vird4 in the 45 kb incu plasmid, pras1, conferring resistance to tetracycline, trimethoprim and sulphonamide, using an in vivo zebrafish infection- treatment model. | 2012 | 22429905 |
| oral and cloacal microflora of wild crocodiles crocodylus acutus and c. moreletii in the mexican caribbean. | bacterial cultures and chemical analyses were performed from cloacal and oral swabs taken from 43 american crocodiles crocodylus acutus and 28 morelet's crocodiles c. moreletii captured in quintana roo state, mexico. we recovered 47 bacterial species (28 genera and 14 families) from all samples with 51.1% of these belonging to the family enterobacteriaceae. fourteen species (29.8%) were detected in both crocodile species and 18 (38.3%) and 15 (31.9%) species were only detected in american and mo ... | 2012 | 22422127 |
| comparative study of semi-specific aeromonas hydrophila and universal pseudomonas fluorescens biosensors for bod measurements in meat industry wastewaters. | aeromonas hydrophila p69.1 (a. hydrophila) was used to construct a semi-specific biosensor to estimate biochemical oxygen demand (bod) in high fat and grease content wastewaters. a. hydrophila cells were grown in fat containing medium to induce necessary enzymes for transport and degradation of fatty substances. universal biosensor based on non-specific pseudomonas fluorescens p75 (p. fluorescens) was used to conduct comparison experiments. biosensors were calibrated using oecd synthetic wastewa ... | 2012 | 22418261 |
| molecular characterization and increased expression of the nile tilapia, oreochromis niloticus (l.), t-cell receptor beta chain in response to streptococcus agalactiae infection. | the complete cdna sequence of the nile tilapia t-cell receptor (tcr) β chain was cloned using 5' race. the full-length, 1263-bp cdna contained a 942-bp open reading frame (orf) encoding a 314-amino-acid protein. sequence analyses revealed that the nile tilapia tcr β chain contains four conserved cysteine residues involved in the formation of disulphide bridges and a conserved amino acid motif believed to be important for assembly and signalling of the tcr αβ/cd3 complex, both of which are normal ... | 2012 | 22417380 |
| dna adenine methyltransferase (dam) controls the expression of the cytotoxic enterotoxin (act) gene of aeromonas hydrophila via trna modifying enzyme-glucose-inhibited division protein (gida). | aeromonas hydrophila is both a human and animal pathogen, and the cytotoxic enterotoxin (act) is a crucial virulence factor of this bacterium because of its associated hemolytic, cytotoxic, and enterotoxic activities. previously, to define the role of some regulatory genes in modulating act production, we showed that deletion of a glucose-inhibited division gene (gida) encoding trna methylase reduced act levels, while overproduction of dna adenine methyltransferase (dam) led to a concomitant inc ... | 2012 | 22391092 |
| aeromonas hydrophila induced head kidney macrophage apoptosis in clarias batrachus involves the activation of calpain and is caspase-3 mediated. | the mechanism of macrophage cytotoxicity induced by aeromonas hydrophila is yet unresolved. we observed a. hydrophila induces head kidney macrophage (hkm) apoptosis in clarias batrachus, as evident from hoechst 33342 and annexinv-propidium iodide staining and presence of oligonucleosomal dna ladder. initiation of apoptosis required the bacteria to be alive, be actively phagocytosed into hkm and was dependent on host proteins. elevated cytosolic calcium and consequent calpain activity that declin ... | 2012 | 22366184 |
| rna-seq analysis of mucosal immune responses reveals signatures of intestinal barrier disruption and pathogen entry following edwardsiella ictaluri infection in channel catfish, ictalurus punctatus. | the mucosal surfaces of fish (gill, skin, gastrointestinal tract) are important sites of bacterial exposure and host defense mechanisms. in mammalian systems, the intestinal epithelium is well characterized as both a selectively permeable barrier regulated by junctional proteins and as a primary site of infection for a number of enteric pathogens including viruses, bacteria, and parasites. the causative bacterium of enteric septicemia of catfish, edwardsiella ictaluri, is believed to gain entry ... | 2012 | 22366064 |
| molecular cloning, characterization and expression of the complement component bf/c2 gene in grass carp. | the complement system is an integral part of the host immune system and plays an immunoregulatory role at the interface between the innate and acquired immune responses. factor b (bf) serves as the catalytic subunit of complement c3 convertase in the alternative pathway (ap), while in the classical pathway (cp), this function is subjected to c2. in this study, we cloned and characterized the two bf/c2 genes of grass carp, gcbf/c2a and gcbf/c2b. the gcbf/c2a and gcbf/c2b cdna sequences are 2259 a ... | 2012 | 22365989 |
| emergence and stability of high-pressure resistance in different food-borne pathogens. | high hydrostatic pressure (hhp) processing is becoming a valuable nonthermal food pasteurization technique, although there is reasonable concern that bacterial hhp resistance could compromise the safety and stability of hhp-processed foods. while the degree of natural hhp resistance has already been shown to vary greatly among and within bacterial species, a still unresolved question remains as to what extent different food-borne pathogens can actually develop hhp resistance. in this study, we t ... | 2012 | 22344661 |
| aeromonas hydrophila septic arthritis. | septic arthritis is a serious, life and limb threatening infection. if suspected, empiric treatment must begin immediately and account for the most likely pathogens. eight days following left knee arthroscopic surgery, a 51-year-old active duty male spent approximately 1 hour driving a personal watercraft on okaloosa bay near the gulf of mexico. eight days later, he presented to the emergency room with septic arthritis of that knee. fluid aspirated from the joint yielded aeromonas hydrophila. th ... | 2011 | 22338363 |
| influence of air quality on the composition of microbial pathogens in fresh rainwater. | in this study, the microbiological quality of fresh rainwater was assessed from 50 rain events under tropical weather conditions for a year. the levels of four major opportunistic waterborne pathogens, namely, escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa, and aeromonas hydrophila, in rainwater samples were quantified by using a robust and sensitive quantitative pcr (qpcr) method. of the 50 rainwater samples, 25 were found to be positive for at least one pathogen: 21 for e. col ... | 2012 | 22327595 |
| effectiveness of bivalent vaccines against aeromonas hydrophila and lactococcus garvieae infections in rainbow trout oncorhynchus mykiss (walbaum). | lactococcus garvieae and aeromonas hydrophila are bacterial pathogens affecting salmonids and other fish species and cause of heavy losses in aquaculture. diseases caused by these bacteria can be controlled satisfactory by immunization using monovalent vaccines. in this study, the protective efficacy of two bivalent vaccines against l. garvieae and a. hydrophila was evaluated in rainbow trout (oncorhynchus mykiss). bivalent formulations, containing formalin-inactivated bacteria, were prepared as ... | 2012 | 22326941 |
| novel microbial route to synthesize zno nanoparticles using aeromonas hydrophila and their activity against pathogenic bacteria and fungi. | in the present work, we describe a low-cost, unreported and simple procedure for biosynthesis of zinc oxide nanoparticles (zno nps) using reproducible bacteria, aeromonas hydrophila as eco-friendly reducing and capping agent. uv-vis spectroscopy, xrd, ftir, afm, nc-afm and fesem with edx analyses were performed to ascertain the formation and characterization of zno nps. the synthesized zno nps were characterized by a peak at 374 nm in the uv-vis spectrum. xrd confirmed the crystalline nature of ... | 2012 | 22321514 |
| [excretion of extracellular membrane nanovesicles by aeromonas]. | study of extracellular membrane nanovesicles production by aeromonas hydrophila and aeromonas salmonicida bacteria on a subcellular level. | 2012 | 22308735 |
| the cytotoxic effect of essential oils from origanum vulgare l. and/or rosmarinus officinalis l. on aeromonas hydrophila. | this study aimed to evaluate the antibacterial activities of the essential oils from origanum vulgare l. (ov) and rosmarinus officinalis l. (ro), both singly and in combination at sub-inhibitory concentrations (¼ mic + ¼ mic), against aeromonas hydrophila and to investigate the possible mechanisms underlying these activities. used singly (ov: 2.5 μl/ml; ro: 20 μl/ml) or in a mixture (ov: 0.625 μl/ml + ro: 5 μl/l), these essential oils led to a significant decrease (p<0.01) in bacterial viability ... | 2012 | 22300224 |