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isolation of bdellovibrio and like organisms and potential to reduce acute hepatopancreatic necrosis disease caused by vibrio parahaemolyticus.acute hepatopancreatic necrosis disease, a severe disease of shrimp, is caused by vibrio parahaemolyticus (ahpnd vp), a halophilic bacterium harboring a plasmid that contains toxin genes homologous to photorhabdus insect-related toxins. we obtained 9 isolates of bdellovibrio and like organisms (balos) from water and sediment samples in thailand. using 16s rrna sequencing, all of the organisms were identified as bacteriovorax spp. and were able to attack all tested ahpnd vp isolates. in addition, ...201728492178
pathogenic vibrio species isolated from estuarine environments (ceará, brazil) - antimicrobial resistance and virulence potential profiles.detection of virulent strains associated with aquatic environment is a current concern for the management and control of human and animal health. thus, vibrio diversity was investigated in four estuaries from state of ceará (pacoti, choró, pirangi and jaguaribe) followed by antimicrobial susceptibility to different antimicrobials used in aquaculture and detection of main virulence factors to human health. isolation and identification were performed on tcbs agar (selective medium) and dichotomous ...201728489191
bile sensing: the activation of vibrio parahaemolyticus virulence.bacteria must develop resistance to various inhospitable conditions in order to survive in the human gastrointestinal tract. bile, which is secreted by the liver, and plays an important role in food digestion also has antimicrobial properties and is able to disrupt cellular homeostasis. paradoxically, although bile is one of the guts defenses, many studies have reported that bacteria such as vibrio parahaemolyticus can sense bile and use its presence as an environmental cue to upregulate virulen ...201728484445
effects of ambient exposure, refrigeration, and icing on vibrio vulnificus and vibrio parahaemolyticus abundances in oysters.vibrio vulnificus (vv) and v. parahaemolyticus (vp) illnesses are typically acquired through the consumption of raw molluscan shellfish, particularly oysters. as vibrio spp. are naturally-occurring bacteria, one means of mitigation of illness is achieved by limiting post-harvest growth. in this study, effects of ambient air storage, refrigeration, and icing of oysters on vibrio spp. abundances were examined at two sites in alabama (al) [dog river (dr) and cedar point (cp)] and one site in delawa ...201728482282
a sulfated galactans supplemented diet enhances the expression of immune genes and protects against vibrio parahaemolyticus infection in shrimp.a sulfated galactans (sg) supplemented diet was evaluated for the potential to stimulate immune activity in shrimp penaeus vannamei (p. vannamei). shrimp given the sg supplemented diet (0.5, 1 and 2% w/w) for 7 days showed enhanced expression of the downstream signaling mediator of lipopolysaccharide and β-1,3-glucan binding protein (lgbp) and immune related genes including p-nf-κb, imd, ikkβ and ikkε, antimicrobial peptide pen-4, propo-i and ii. following immersion with vibrio parahaemolyticus ...201728442419
three new piscidins from orange-spotted grouper (epinephelus coioides): phylogeny, expression and functional characterization.the present study reports the identification, and characterization of three new putative piscidin paralogues, ecpis-2, ecpis-3 and ecpis-4, from orange-spotted grouper (epinephelus coioides). the cdna of the three piscidins with the 207, 216, and 231 nt open reading frame encoded respectively a 68-, 71-, and 76-amino acid preprotein consisting of the predicted signal peptide, and putative mature peptide and prodomain. the phylogenetic analysis indicated that multiple piscidin paralogues in one f ...201728433718
acute hepatopancreatic necrosis disease-causing vibrio parahaemolyticus strains maintain an antibacterial type vi secretion system with versatile effector repertoires.acute hepatopancreatic necrosis disease (ahpnd) is a newly emerging shrimp disease that has severely damaged the global shrimp industry. ahpnd is caused by toxic strains of vibrio parahaemolyticus that have acquired a "selfish plasmid" encoding the deadly binary toxins pira(vp)/pirb(vp) to better understand the repertoire of virulence factors in ahpnd-causing v. parahaemolyticus, we conducted a comparative analysis using the genome sequences of the clinical strain rimd2210633 and of environmenta ...201728432099
recombinant pira-like toxin protects shrimp against challenge with vibrio parahaemolyticus, the aetiological agent of acute hepatopancreatic necrosis disease. 201728422302
plasmid dynamics in vibrio parahaemolyticus strains related to shrimp acute hepatopancreatic necrosis syndrome (ahpns).vibrio parahaemolyticus is a causative agent of acute hapatopancreatic necrosis syndrome (ahpns) which causes early mortality in white shrimp. emergence of ahpns has caused tremendous economic loss for aquaculture industry particularly in asia since 2010. previous studies reported that strains causing ahpns harbor a 69-kb plasmid with possession of virulence genes, pira and pirb. however, genetic variation of the 69-kb plasmid among ahpns related strains has not been investigated. this study aim ...201728404482
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