| [t cells recognizing ebv-epitopes arose in co-culture of peripheral blood mononuclear cells from eb-infected patients and dendritic cells loaded with lmp2-mixed peptides]. | the latent membrane protein 2 (lmp2) is a kind of protein expressed by ebv-infected cells. this study was aimed to investigate whether the stimulation of peripheral blood mononuclear cells with peptides induces ebv-specific cytotoxic t lymphocytes (ctl). the peptides were mixture of lmp2 protein and available for people with different hla types. peripheral blood sample was collected from a patient with ebv-associated hemophagocytic syndrome. the mononuclear cells were isolated and cultured to ob ... | 2008 | 18426672 |
| establishment of permanent cell lines by epstein-barr virus transformation. | this basic protocol presents epstein-barr virus (ebv) transformation of b cells to create a permanent cell line to provide cells for genetic and dna analysis. virus stocks are prepared from cultures of an infected marmoset cell line in the first support protocol. transformed cells may be analyzed immediately or frozen for future use as described in the second support protocol. | 2001 | 18428224 |
| age-related epstein-barr virus-associated b-cell lymphoproliferative disorders: special references to lymphomas surrounding this newly recognized clinicopathologic disease. | epstein-barr virus (ebv) is associated with some disease entities of malignant lymphomas, including burkitt lymphoma, hodgkin lymphoma, immunodeficiency-associated lymphoproliferative disorders (lpd), and a part of diffuse large b-cell lymphoma. we have recently identified a series of elderly patients with ebv-associated (or ebv(+)) b-cell lpd (b-lpd) showing similarities in many respects to immunodeficiency-associated lpd, although no evidence of underlying immunodeficiency was found. therefore ... | 2008 | 18429953 |
| the immunology of multiple sclerosis: disease mechanisms and therapeutic targets. | the disease mechanism of multiple sclerosis (ms) involves inflammation, demyelination and neurodegeneration. the relation between these components is not completely understood, but recent experiences with aggressive anti-inflammatory treatment suggest that inflammation drives neuronal damage in patients with relapsing remitting ms. although infiltration of lymphocytes into the brain parenchyma was recognized as a key event in the pathogenesis of ms more than 120 years ago, important aspects of t ... | 2008 | 18431322 |
| hla type of ebv-transformed human b cell lines. | this appendix lists reference epstein-barr virus (ebv)-transformed b lymphoblastoid cell lines (b-lcl) and the hla types expressed by these cell lines. these b-lcl are useful for studies of cellular immunity (e.g., restriction of antigen-specific responses), biochemical characterization of histocompatibility molecules, and as controls for dna-based typing methods. | 2001 | 18432643 |
| generation of epstein-barr virus (ebv)-immortalized b cell lines. | immortalization of b lymphocytes by ebv is an effective procedure for inducing long-term growth of certain human b lymphocytes. the protocol described in this unit to accomplish this can be divided into three stages: preparation of virus, preparation of target cells to be immortalized, and ebv infection and growth of infected cells. | 2007 | 18432996 |
| b-cell non-hodgkin lymphomas in uganda: an immunohistochemical appraisal on tissue microarray. | the most common non-hodgkin lymphomas in uganda are neoplasms of b-cell derivation. the field of b-cell lymphoma immunophenotype has rapidly progressed because of the increasing availability of markers applicable to routine sections. although the latter have allowed the identification of distinctive lymphoma entities in the developed countries, such approach has not yet been used in uganda. one hundred twenty-nine formalin-fixed, paraffin-embedded tissue samples from the department of pathology ... | 2008 | 18436278 |
| immune surveillance of ebv-infected b cells and the development of non-hodgkin lymphomas in immunocompromised patients. | after infection with the epstein - barr virus, a common gammaherpes virus which infects and persists in the b cells, an equilibrium is established in which newly infected and differentiating b cells are controlled by cytotoxic t cell (ctl) responses. disturbance of this equilibrium, which can occur in immunocompromised situations, can lead to uncontrolled lymphoproliferation and subsequent development of non-hodgkin lymphomas (nhl). here, we review the role of immunesurveillance of ebv-infected ... | 2008 | 18452077 |
| the predictive value of cerebrospinal fluid epstein-barr viral load as a marker of primary central nervous system lymphoma in hiv-infected persons. | the presence of epstein-barr virus (ebv) dna in cerebrospinal fluid (csf) is used as a marker of hiv-associated primary central nervous system lymphoma (pcnsl). in our setting, ebv dna is frequently detected in the csf of hiv-infected patients with miscellaneous neurological diseases and thus its presence is a poor predictor of pcnsl. | 2008 | 18455472 |
| evaluation of commercial ebv recombline assay for diagnosis of nasopharyngeal carcinoma. | in recent years a number of epstein-barr virus (ebv) proteins were defined as being immunodominant for either igm, igg or iga immune responses, yielding promising markers for diagnostic serology. specific reactivity patterns to these proteins have been described for infectious mononucleosis (im), nasopharyngeal carcinoma (npc), various types of lymphoma, and healthy ebv carriers. | 2008 | 18455473 |
| epstein-barr virus-associated lymphoepithelioma-like carcinoma of mandible. | we describe the first case of an lymphoepithelioma-like carcinoma (lec) of the mandible with a view to discuss probable histogenesis from salivary gland inclusions in the mandible, effective therapy, the unusual pattern of nodal metastases, and association with the epstein-barr virus. an 11-year-old boy presented with a lytic lesion in the left mandible and bilateral neck node enlargement. histologically, the biopsied metastatic neck nodes showed sinusoids distended with histiocytes and single l ... | 2009 | 18457481 |
| promising approach for aggressive nk cell leukaemia with allogeneic haematopoietic cell transplantation. | aggressive natural killer cell leukaemia (ankl) is a malignant disorder of mature nk cells with a poor prognosis, for which no effective therapeutic approach has been established. we investigated the role of allogeneic haematopoietic cell transplantion (allo-hct) in ankl. | 2008 | 18462253 |
| epstein-barr virus sm protein functions as an alternative splicing factor. | alternative splicing of rna increases the coding potential of the genome and allows for additional regulatory control over gene expression. the full extent of alternative splicing remains to be defined but is likely to significantly expand the size of the human transcriptome. there are several examples of mammalian viruses regulating viral splicing or inhibiting cellular splicing in order to facilitate viral replication. here, we describe a viral protein that induces alternative splicing of a ce ... | 2008 | 18463151 |
| antitumor activity of ebv-specific t lymphocytes transduced with a dominant negative tgf-beta receptor. | transforming growth factor (tgf)-beta is produced in most human tumors and markedly inhibits tumor antigen-specific cellular immunity, representing a major obstacle to the success of tumor immunotherapy. tgf-beta is produced in epstein-barr virus (ebv)-positive hodgkin disease and non-hodgkin lymphoma both by the tumor cells and by infiltrating t-regulatory cells and may contribute the escape of these tumors from infused ebv-specific t cells. to determine whether tumor antigen-specific cytotoxic ... | 2008 | 18463534 |
| herpesviruses and autoimmunity. | herpesvirus infection, in particular ebv infection, has been implicated in several major autoimmune diseases, including systemic lupus erythematosus (sle), multiple sclerosis (ms) and rheumatoid arthritis (ra). herpesvirus infection has potential roles in both initiating the autoimmune process and exacerbating disease progression. in particular, ebv has a proposed role in initiating the anti-nucleoprotein antibodies that are characteristic of sle through molecular mimicry. there is also evidence ... | 2008 | 18465661 |
| differentiation of ebv-induced post-transplant hodgkin lymphoma from hodgkin-like post-transplant lymphoproliferative disease. | the development of lymphomas after sot is a well-known complication of the immunosuppressive therapy necessary to prevent graft rejection. epstein-barr virus plays a central role in the pathogenesis of lymphomas because of its ability to transform infected cells. differentiating ptld from malignant lymphomas, especially hl can be challenging. we report on two patients, who developed ebv-associated lymphomas several years after sot. a histological examination of lymph nodes led to a diagnosis of ... | 2008 | 18466428 |
| screening for ptld in lung and heart-lung transplant recipients by measuring ebv dna load in bronchoalveolar lavage fluid using real time pcr. | pediatric l-hltx recipients are at risk for developing ptld with the lung being a primary site of disease. we hypothesized that balf is a better sample than peripheral blood for measuring ebv dna load in this high-risk population. archived balf specimens from pediatric l-hltx recipients with and without ptld were assayed for ebv dna load using a quantitative real time taqman pcr assay. these values were compared with values determined in peripheral blood by a competitive pcr assay. fifty-five ba ... | 2008 | 18466434 |
| lymphomatoid granulomatosis involving central nervous system successfully treated with rituximab alone. | to report the successful treatment of a patient with lymphomatoid granulomatosis (lyg), a rare epstein-barr virus-positive lymphoproliferative disorder, using rituximab (anti-cd20 monoclonal antibody). the prognosis for lyg has been reported to be poor, and no satisfactory treatment has been established. because central nervous system (cns) involvement of lyg has been known to show poor prognosis, the establishment of an effective treatment for cns lyg with mild adverse effects is desired. | 2008 | 18474745 |
| [analysis of ebv-lmp1 gene mutation in plasma of patients with nasopharyngeal carcinoma in chongqing]. | to detect and analyze the ebv-lmp1 gene n terminus xhoi-site mutation and c terminus 30 bp deletion in plasma of patients with nasopharyngeal carcinoma (npc) in chongqing of china. | 2008 | 18476617 |
| [gene modification in the genome of epstein-barr virus cloned as a bacterial artificial chromosome]. | epstein-barr virus (ebv) is an oncogenic herpesvirus associated with a variety of malignancies, including burkitt's lymphoma and nasopharyngeal carcinoma (npc). functions of most ebv genes have not been determined. the use of bacterial artificial chromosome (bac) to clone and modify the genome of ebv has enhanced the gene function study in the context of genome. infectious clones of ebv were previously established by using ebv-bac plasmid p2089. in order to further investigate ebv mutant biology ... | 2008 | 18479068 |
| [proliferation cycle changes of epstein-barr virus in nasopharyngeal carcinoma cell line cne after infection]. | the strategy of gene therapy on nasopharyngeal carcinoma (npc) is determined by the characteristics of proliferation cycle of epstein-barr virus (ebv) in different histological types of npc cells. this study was to investigate the characteristics of proliferation cycle of ebv in npc cell line cne2. | 2008 | 18479592 |
| effect of epstein-barr virus sequence variation on performance of the roche real-time lightcycler pcr assay. | | 2008 | 18480228 |
| dna hypermethylation contributes to incomplete synthesis of carbohydrate determinants in gastrointestinal cancer. | it has long been known that malignant transformation is associated with abnormal expression of carbohydrate determinants. the aim of this study was to clarify the cause of cancer-associated abnormal glycosylation in gastrointestinal (gi) cancers. | 2008 | 18485915 |
| comparison of epstein-barr virus genotypes and clinicohistopathological features of nasopharyngeal carcinoma between guilin, china and fukuoka, japan. | epstein-barr virus (ebv)-associated nasopharyngeal carcinoma (npc) is endemic to guilin, china, but not fukuoka, japan. to examine whether the npc in these two cities are distinct in their association with ebv, we analyzed the histology and ebv genotypes in 163 npc from guilin, 52 npc from fukuoka and non-cancerous control nasopharyngeal tissues (n=22 each) by in situ hybridization and pcr. the proportion of ebv-positive npc from guilin (95%) was higher than that of fukuoka (55%, p<0.001), and h ... | 2008 | 18497945 |
| expression of the epstein-barr virus-encoded epstein-barr virus nuclear antigen 1 in hodgkin's lymphoma cells mediates up-regulation of ccl20 and the migration of regulatory t cells. | in approximately 50% of patients with hodgkin's lymphoma (hl), the epstein-barr virus (ebv), an oncogenic herpesvirus, is present in tumor cells. after microarray profiling of both hl tumors and cell lines, we found that ebv infection increased the expression of the chemokine ccl20 in both primary hodgkin and reed-sternberg cells and hodgkin and reed-sternberg cell-derived cell lines. additionally, this up-regulation could be mediated by the ebv nuclear antigen 1 protein. the higher levels of cc ... | 2008 | 18502823 |
| ebv-positive immunodeficiency lymphoma after alemtuzumab-chop therapy for peripheral t-cell lymphoma. | chemotherapy with alemtuzumab and the combination of cyclophosphamide, adriamycin, oncovin, and prednisone (chop) has become experimental trial therapy for aggressive t-cell lymphoma. several multicenter phase 3 trials will incorporate this scheme. as part of an ongoing phase 2 trial in which we recently treated 20 patients with 8 cycles of chop every 2 weeks with 3 additional doses of 30 mg alemtuzumab per cycle, we observed the development of epstein-barr virus (ebv)-positive lymphoproliferati ... | 2008 | 18502831 |
| the epstein-barr virus (ebv) protein eb is an mrna export factor essential for virus production. | the ebv early protein eb2 (aka mta, sm and bmlf1) shares properties with mrna export factors. it shuttles between the cytoplasm and the nucleus, and interacts with rna both in vitro and in vivo but with no apparent sequence specificity. eb2 induces the cytoplasmic accumulation of mrnas generated from intronless and intron-containing genes, likely through interactions with cellular export factors of the tap/p15 pathway. using a cell line carrying a viral genome with the eb2 gene deleted, it has b ... | 2008 | 18508475 |
| epstein-barr virus persistence in the absence of conventional memory b cells: igm+igd+cd27+ b cells harbor the virus in x-linked lymphoproliferative disease patients. | epstein-barr virus (ebv) persists in healthy virus carriers within the immunoglobulin (ig)d(-)cd27(+) (class-switched) memory b-cell compartment that normally arises through antigen stimulation and germinal center transit. patients with x-linked lymphoproliferative disease (xlp) lack such class-switched memory b cells but are highly susceptible to ebv infection, often developing fatal symptoms resembling those seen in ebv-associated hemophagocytic syndrome (ebv-ahs), a disease caused by aberrant ... | 2008 | 18509091 |
| ebv reactivation and post transplant lymphoproliferative disorders following allogeneic sct. | fatal problems encountered in allogeneic stem cell transplantation include ebv reactivation and post transplant lymphoproliferative disorders (ptlds) with high mortality rates. we performed a retrospective analysis in all consecutive adult and pediatric ebv reactivations and ptld during a period of 8.5 years. there were 26 patients with ebv reactivation/ptld out of a total of 854 transplantations giving an overall incidence of 3.0%. specifically, the incidence of ebv-ptld was 1.3%, whereas that ... | 2008 | 18516079 |
| [epstein-barr virus-associated b cell lymphoproliferative disorder complicated in adult t-cell leukemia]. | adult t-cell leukemia (atl) is accompanied by remarkably impaired cellular immune responses. reports on the simultaneous development of atl and epstein-barr virus-associated lymphoproliferative disorders (ebv-lpd) are, however, quite rare. a 53-years-old man was admitted to our hospital because of multiple bilateral pulmonary nodules, skin rash, lymphadenopathy, and hypercalcemia. the smear preparation of peripheral blood demonstrated pathological lymphocytes with lobulated nuclei. they expresse ... | 2008 | 18516870 |
| theodore e. woodward award: development of novel, ebv-targeted therapies for ebv-positive tumors. | the near universal presence of ebv in certain tumors suggests that new ebv-based therapies could be developed for these malignancies. we have explored one ebv-based therapy that involves the purposeful induction of lytic ebv infection in tumors. induction of lytic ebv infection in tumors activates expression of ebv-encoded kinases that convert the prodrug, ganciclovir, to its active cytotoxic form. in mouse models for ebv-positive tumors, the combination of lytic-inducing chemotherapy and gancic ... | 2006 | 18528464 |
| malignant hematopoietic cell lines: in vitro models for the study of waldenström's macroglobulinemia. | model cell lines are essential tools for investigating the biology and therapeutics of cancer. approximately 1500 human hematopoietic neoplastic cell lines have been described, covering most major disease entities. waldenström's macroglobulinemia (wm) is a rare incurable hematological neoplasm from which only three cell lines have been derived. mindful that candidate tumor cell lines sometimes arise spuriously by viral immortalization of bystander cells, we review the extent to which wm cell lin ... | 2008 | 18533260 |
| epstein-barr virus infection in pregnancy--a prospective controlled study. | epstein-barr virus (ebv) is one of the most common human viruses. to date, there is limited information regarding the influence of maternal ebv infection on pregnancy outcome. | 2008 | 18534816 |
| breast cancer and microbial cancer incidence in female populations around the world: a surprising hyperbolic association. | current literature on cancer epidemiology typically discusses etiology of cancer by cancer type. risks of different cancer types are, however, correlated at population level and may provide etiological clues. we showed previously an unexpected very high positive correlation between breast cancer (bc) and young-adult hodgkin disease incidence rates. in a population-based case-control study of bc, older ages at the first epstein-barr virus exposure, indicated by older ages at onset of infectious m ... | 2008 | 18537160 |
| lymphoid chemokines in chronic neuroinflammation. | lymphoid chemokines play an essential role in the establishment and maintenance of lymphoid tissue microarchitecture and have been implicated in the formation of tertiary (or ectopic) lymphoid tissue in chronic inflammatory conditions. here, we review recent advances in lymphoid chemokine research in central nervous system inflammation, focusing on multiple sclerosis and the animal model experimental autoimmune encephalomyelitis. we also highlight how the study of lymphoid chemokines, particular ... | 2008 | 18539341 |
| update on molecular diagnostic tests in head and neck cancer. | head and neck cancers (hncs) include several cancers originating in the upper airways that represent a variety of histologies. the most common type of hnc is squamous cell carcinoma (scc), which is linked to tobacco and alcohol use and to human papilloma virus (hpv). at present, there are no standard molecular tests that are routinely used in clinics. this overview will discuss the current knowledge on molecular markers with the potential to be developed as diagnostic tests for cancer risk asses ... | 2008 | 18544435 |
| spontaneous t cell responses to epstein-barr virus-encoded barf1 protein and derived peptides in patients with nasopharyngeal carcinoma: bases for improved immunotherapy. | immunotherapy approaches targeting epstein-barr virus (ebv)-encoded antigens induce objective clinical responses only in a fraction of patients with undifferentiated nasopharyngeal carcinoma (unpc). in the present study, we have characterized the immunogenicity of the ebv-encoded barf1 oncogene with the aim to assess whether this protein could constitute a new target antigen for immunotherapy in this setting. spontaneous cd4+ and cd8+ t cell responses specific for the recombinant p29 barf1 prote ... | 2008 | 18546263 |
| strong ebv-specific cd8+ t-cell response in patients with early multiple sclerosis. | epstein-barr virus (ebv) has been associated with multiple sclerosis (ms), however, most studies examining the relationship between the virus and the disease have been based on serologies, and if ebv is linked to ms, cd8+ t cells are likely to be involved as they are important both in ms pathogenesis and in controlling viruses. we hypothesized that valuable information on the link between ms and ebv would be ascertained from the study of frequency and activation levels of ebv-specific cd8+ t cel ... | 2008 | 18550621 |
| correlation between reduction potentials and inhibitions of epstein-barr virus activation by anthraquinone derivatives. | as a continuation of studies using natural and synthetic products as cancer chemopreventive agents, we used cyclic voltammetry to examine the reduction-oxidation potentials of methylated emodin derivatives prepared from emodin in phosphate buffer at ph 7.2. a good correlation was found between the inhibitory effects on epstein-barr virus early antigen (ebv-ea) activation and the reduction potential of methylated emodin derivatives. furthermore, there was significant correlation between ebv-ea ac ... | 2008 | 18556196 |
| the epstein-barr virus lf2 protein inhibits viral replication. | the switch from epstein-barr virus (ebv) latent infection to lytic replication is governed by two transcriptional regulators, zta and rta. we previously reported that the ebv protein encoded by the lf2 gene binds to rta and can inhibit rta activity in reporter gene assays. we now report that lf2 associates with rta in the context of ebv-infected cells induced for lytic replication. lf2 inhibition of rta occurs in both epithelial and b cells, and this downregulation is promoter specific: lf2 decr ... | 2008 | 18562535 |
| detection and quantitative analysis of human herpesvirus in pilocytic astrocytoma. | we investigated the hypothetical role of human herpesviruses (hhvs) in tumour formation of the cerebellum. thirty-five samples of pilocytic astrocytoma and 10 control samples of cerebellum from patients who died of unrelated diseases were examined. presence of the 8 known hhvs was first studied using specific real-time quantitative polymerase chain reaction (qpcr) targeting viral dna polymerase. hhv's dna polymerase was found present in 20 samples (7 controls, 13 astrocytomas) and was absent in ... | 2008 | 18565499 |
| prevalence of human herpes virus types 1-7 in the semen of men attending an infertility clinic and correlation with semen parameters. | to determine the prevalence of herpes viruses in the semen of an asymptomatic male cohort with and without infertility problems and its association with altered semen parameters. | 2009 | 18565516 |
| association of helicobacter pylori and epstein-barr virus with gastric cancer and peptic ulcer disease. | helicobacter pylori and epstein-barr virus (ebv) infections are common world-wide. though h. pylori infection is a major factor in gastroduodenal diseases, its role in association with ebv infection is unknown. we prospectively studied the association of h. pylori and ebv in patients with gastric cancer (gc) and peptic ulcer disease (pud). | 2008 | 18569983 |
| human herpesvirus 8 infections in patients with immunodeficiencies. | in 1994, chang et al described a novel herpesvirus in tissues from patients with kaposi sarcoma, referred to as kaposi sarcoma herpesvirus or human herpesvirus 8. they used a very sophisticated technique of molecular biology to isolate unknown dna sequences from kaposi sarcoma lesions, which were not present in normal tissues. it turned out that these sequences corresponded to a previously unrecognized gamma herpesvirus highly homologous to human herpesvirus 4 (epstein-barr virus) and to herpesv ... | 2008 | 18570975 |
| epstein-barr virus latent membrane protein 2a preferentially signals through the src family kinase lyn. | latent membrane protein 2a (lmp2a) is a viral protein expressed during epstein-barr virus (ebv) latency in ebv-infected b cells both in cell culture and in vivo. lmp2a has important roles in modulating b-cell receptor signal transduction and provides survival and developmental signals to b cells in vivo. although lyn has been shown to be important in mediating lmp2a signaling, it is still unclear if lyn is used preferentially or if lmp2a associates promiscuously with other src family kinase (sfk ... | 2008 | 18579586 |
| [the possible curative therapy for rheumatoid arthritis--ebv infection control gene sap and its application]. | epstein-barr virus (ebv) belong to herpes virus group. this virus is transmitted by human contact and cause primary infection and may exist even for years in a latent state in healthy individuals. this virus may be reactivated by the dysregulation of the host immune system or possibly by virus mutation. here we have firstly demonstrated the host defense of ebv infection and association of ebv with rheumatoid synovitis, and then discussed our own ideas of the possible treatment in near future. th ... | 2008 | 18587224 |
| tonsillar lesions of infectious mononucleosis resembling malt type lymphoma. a report of two cases. | infectious mononucleosis (im) is an acute lymphoproliferative disorder that typically occurs in young patients and is usually caused by epstein-barr virus. we report here, two cases of tonsillar lesion of im resembling marginal zone b-cell lymphoma mucosa-associated lymphoid tissue (malt) type. the patients consisted of an 18-year-old japanese woman and a 36-year-old japanese man. both patients presented with tonsillar mass. histologically, in one case, the tonsil showed diffuse proliferation of ... | 2008 | 18592404 |
| the presence or absence of latent epstein-barr virus does not alter the sensitivity of burkitt's lymphoma cell lines to proteasome inhibitors. | | 2008 | 18594134 |
| human osteoclast-poor osteopetrosis with hypogammaglobulinemia due to tnfrsf11a (rank) mutations. | autosomal-recessive osteopetrosis (aro) comprises a heterogeneous group of bone diseases for which mutations in five genes are known as causative. most aro are classified as osteoclast-rich, but recently a subset of osteoclast-poor aro has been recognized as due to a defect in tnfsf11 (also called rankl or trance, coding for the rankl protein), a master gene driving osteoclast differentiation along the rankl-rank axis. rankl and rank (coded for by the tnfrsf11a gene) also play a role in the immu ... | 2008 | 18606301 |
| cyclosporin a-induced lipid and protein oxidation in human b-cells and in epstein-barr virus-infected b-cells is prevented by antioxidants. | the incidence of post-transplant lymphoproliferative disorder (ptld) has increased since cyclosporin a (csa) became the mainstay of transplant immunosuppression. we have previously shown that, in addition to its potent immunosuppressive property, csa-induced oxidative stress plays an important role in epstein-barr virus (ebv)-related ptld. using lipid hydroperoxide and malondialdehyde as markers of lipid oxidation, and protein carbonyls as markers of protein oxidation, we further investigated th ... | 2008 | 18615317 |
| plasma epstein-barr viral load predicting response after chemotherapy for post-transplant lymphoproliferative disease. | transplant patients are particularly at risk of developing b post-transplant lymphoproliferative disease (ptld) related to intensive immunosuppressive treatment to prevent graft rejection. in ebv-positive ptlds, ebv-dna can be found in the patients' peripheral blood. several methods have been described to assess peripheral blood ebv viral load. we report a case of a 13-year-old child who developed ebv-positive ptld after renal transplantation. we assessed ebv plasma viral load by quantitative pc ... | 2008 | 18618224 |
| a high viral load of epstein-barr virus dna in ocular fluids in an hla-b27-negative acute anterior uveitis patient with psoriasis. | | 2008 | 18626741 |
| chronic active epstein-barr virus infection with mosquito allergy successfully treated with reduced-intensity unrelated allogeneic bone marrow transplantation in a boy. | ebv-infected t-/nk cells play an important role in the pathogenesis of mosquito allergy, and the prognosis of most patients with mosquito allergy is poor without proper treatment. we describe a 13-yr-old boy who had caebv with mosquito allergy and was successfully treated with bmt from an unrelated donor after reduced-intensity preconditioning. because combination chemotherapy failed to achieve cr, we performed unrelated bmt to reconstitute normal immunity and eradicate any residual ebv-infected ... | 2009 | 18627511 |
| could other viruses cause pediatric posttransplant lymphoproliferative disorder? | posttransplant lymphoproliferative disorder (ptld) constitutes a heterogeneous group of diseases. we summarize the experience of our hospital, one of spain's largest series of renal (294), liver (47) and allogeneic stem cell transplants (67), where four cases of ptld have developed related to complex viral infections. | 2008 | 18628071 |
| the reversal of epigenetic silencing of the ebv genome is regulated by viral bzip protein. | ebv (epstein-barr virus) alternates between latency and lytic replication. during latency, the viral genome is largely silenced by host-driven methylation of cpg motifs and in the switch to the lytic cycle this epigenetic silencing is overturned. a key event is the activation of the viral protein zta with three zres (zta-response elements) from the brlf1 promoter (referred to as rp). two of these zres contain cpg motifs and are methylated in the latent genome. biochemical analyses and molecular ... | 2008 | 18631132 |
| systemic ebv+ t-cell lymphoma in elderly patients: comparison with children and young adult patients. | fulminant epstein-barr virus (ebv+) t-cell lymphoma in immunocompetent elderly patients is rare and its character has not been well defined. this study analyzed the clinicopathological features of five elderly patients (group a: 50-84 years) and compared them with those of eight children and young adult patients with systemic t-cell lymphomas (group b: 10-34 years). group a more commonly presented with generalized lymphadenopathy (n = 3) than did group b (n = 1). chronic active ebv infection (n ... | 2008 | 18636273 |
| racial/ethnic variation in ebv-positive classical hodgkin lymphoma in california populations. | epstein-barr virus (ebv) is detected in the tumor cells of some but not all hodgkin lymphoma (hl) patients, and evidence indicates that ebv-positive and -negative hl are distinct entities. racial/ethnic variation in ebv-positive hl in international comparisons suggests etiologic roles for environmental and genetic factors, but these studies used clinical series and evaluated ebv presence by differing protocols. therefore, we evaluated ebv presence in the tumors of a large (n = 1,032), racially a ... | 2008 | 18646185 |
| [characteristics of epstein-barr virus-associated t/nk-cell lymphoproliferative disease]. | | 2008 | 18646606 |
| epstein-barr virus polymerase chain reaction-negative stage iv post-transplant lymphoproliferative disorder in a heart transplant patient treated with rituximab. | post-transplant lymphoproliferative disorder (ptld) is often associated with epstein-barr virus (ebv). however, in this report, we describe a heart transplant recipient with pathologically proven ebv-positive ptld with undetectable virus by polymerase chain reaction. the patient had remission after anti-cd20 antibody treatment early after transplantation. | 2008 | 18656811 |
| differential expression of cd26 on virus-specific cd8(+) t cells during active, latent and resolved infection. | the hallmark of effective establishment of immune memory is the long-term memory cell that persists in the absence of antigen. to explore its characteristics, we investigated the differences between a resolved successful immune response, such as after influenza (flu) vaccination, and the state of chronic infection with persistent antigen, such as with cytomegalovirus (cmv), epstein-barr virus (ebv) or human immunodeficiency virus (hiv), which leads to defective t-cell memory. immunophenotypic an ... | 2009 | 18657205 |
| [epstein-barr infections in children with acute leukaemia. preliminary report]. | epstein-barr virus (ebv) infection is common all over the world. primary ebv infection can lead to latent or chronic infection resulting in lymphoproliferative diseases. | 2008 | 18663268 |
| cd137-guided isolation and expansion of antigen-specific cd8 cells for potential use in adoptive immunotherapy. | the efficient isolation and ex vivo expansion of antigen-specific t cells are crucial for successful adoptive immunotherapy against uncontrollable infections and cancers. several methods have been reported for this purpose, for example, employing mhc-multimeric complexes, interferon-gamma secretion, and antibodies specific for molecules expressed on t-cell surfaces, including cd25, cd69, cd107a, cd137, and cd154. of the latter, cd137 has been shown to be one of the most promising targets since i ... | 2008 | 18677654 |
| kaposi's sarcoma-associated herpesvirus (kshv) rta and cellular hmgb1 proteins synergistically transactivate the kshv orf50 promoter. | kaposi's sarcoma-associated herpesvirus 'replication transcriptional activator' (rta) plays a critical role in the switch from latency to lytic replication. rta upregulates several lytic kshv genes, including its own, through multiple mechanisms. we demonstrate that cellular hmgb1 binds and synergistically upregulates the orf50 promoter in conjunction with rta. no direct interaction between rta and hmgb1 was observed, however a ternary complex is formed in the presence of oct1. furthermore, dele ... | 2008 | 18692049 |
| maribavir: a novel antiviral agent with activity against cytomegalovirus. | to review the pharmacology, pharmacokinetics, efficacy, and safety of maribavir, a novel antiviral agent in the benzimidazole drug class. | 2008 | 18698013 |
| sat-nav virology. | | 2008 | 18702084 |
| cytologic diagnosis of hemophagocytic lymphohistiocytosis in ascitic fluid: a case report. | hemophagocytic lymphohistiocytosis (hlh), also known as macrophage activation syndrome, is a rare, fatal hematopoietic disease. its cytologic features may be subtle because the abnormal histiocytes may not be recognized if one is not aware of this entity. we report a case of hlh involving the ascitic fluid. | 2008 | 18702370 |
| follicular lymphoma in a x-linked lymphoproliferative syndrome carrier female. | x-linked lymphoproliferative (xlp) syndrome is a rare primary immune-deficiency disorder caused by mutations of the sh2d1a or xiap genes. males with the disorder are usually in good health until contracting epstein-barr virus (ebv) whereupon the majority of patients die from fulminant infectious mononucleosis, lymphoma or hypogammaglobulinaemia. this report describes a female carrier with an xlp phenotype who was retrospectively identified after her grandson died from the disorder. subsequent ge ... | 2008 | 18702745 |
| influence of epstein-barr virus infection in adult t-cell leukemia. | the involvement of adult t-cell leukemia (atl) cells in organs such as the skin and lymph nodes is observed in about 50% of cases of atl. epstein-barr virus (ebv) infection has often been observed in the clinical course of atl. in this study, we established two b-cell lines from peripheral blood of patients with atl. ebv dna, proviral dna for htlv-1 and tax mrna were detected in both lines. as part of the characterization of these cells, an enhanced expression of intercellular adhesion molecule- ... | 2008 | 18702873 |
| infections as triggers and complications of systemic lupus erythematosus. | a growing body of experimental and clinical evidence supports the pivotal role of infections in the induction or exacerbation of systemic lupus erythematosus (sle). infections can be responsible for aberrant immune response leading to a loss of tolerance towards native proteins. molecular mimicry, especially between sm or ro autoantigens and ebv nuclear antigen-1 response, as well as the over-expression of type 1 inf genes are among the major contributors to sle development. on the other hand in ... | 2008 | 18703174 |
| anchor-based fluorescent amplicon generation assays (flag) for real-time measurement of human cytomegalovirus, epstein-barr virus, and varicella-zoster virus viral loads. | monitoring the human cytomegalovirus (hcmv), epstein-barr virus (ebv), or varicella-zoster virus (vzv) viral load is an important factor in the management of immunosuppressed patients, such as recipients of solid-organ or bone marrow transplants. the advent of real-time pcr technologies has prompted the widespread development of quantitative pcr assays for the detection of viral loads and other diagnostic purposes. | 2008 | 18703767 |
| establishment and characterization of a new human acute myelocytic leukemia cell line sh-2 with a loss of y chromosome, a derivative chromosome 16 resulting from an unbalanced translocation between chromosomes 16 and 17, monosomy 17, trisomy 19, and p53 alteration. | to report here a new acute myelocytic leukemia (aml) cell line sh-2 and describe its biological characteristics. | 2008 | 18715689 |
| epstein-barr virus infection and plasma transforming growth factor-beta1 levels in head and neck cancers. | in addition to the correlation of transforming growth factor (tgf)-beta1 levels to the radiation toxicity, both epstein-barr virus (ebv) infection and postoperative status play roles in the change in plasma tgf-beta1 levels in patients with head and neck cancers. | 2008 | 18720060 |
| lung involvement in igg4-related lymphoplasmacytic vasculitis and interstitial fibrosis: report of 3 cases and review of the literature. | there have been a few reports on lung diseases associated with an increased number of infiltrating igg4-positive plasma cells or lung involvement of igg4-related sclerosing disease, although their characteristic histologic features have not been well described. herein, we present 3 cases of interstitial lung disease with common histology and abundant igg4-positive cell infiltration. patient 1, a 65-year-old man, was incidentally noted to have a nodular lesion in the left hilar region. in patient ... | 2008 | 18753944 |
| hiv-associated plasmablastic lymphoma: lessons learned from 112 published cases. | plasmablastic lymphoma (pbl) is a distinct subtype of non-hodgkin b-cell lymphoma, originally described with a strong predilection to the oral cavity of human immunodeficiency virus (hiv)-infected individuals. data regarding patient age and gender, hiv status, initiation of and response to highly active antiretroviral therapy (haart), tumor extent, pathology, treatment, and outcome were extracted from 112 cases of pbl identified in the literature. the median age at presentation was 38 years with ... | 2008 | 18756521 |
| rapid identification and sorting of viable virus-reactive cd4(+) and cd8(+) t cells based on antigen-triggered cd137 expression. | current methods for the detection and isolation of antigen-specific cd4(+) and cd8(+) t cells require the availability of peptide/mhc multimers or are restricted to cells that produce cytokines after antigen contact. here we show that de novo cell surface expression of the tnf-receptor family member cd137 (4-1bb) identifies recently activated, but not resting, human cd4(+) and cd8(+) memory t cells. maximum cd137 expression level is uniformly observed in both t-cell subsets at 24h after stimulat ... | 2008 | 18760281 |
| bortezomib-induced enzyme-targeted radiation therapy in herpesvirus-associated tumors. | we investigated the possibility of using a pharmacologic agent to modulate viral gene expression to target radiotherapy to tumor tissue. in a mouse xenograft model, we had previously shown targeting of [(125)i]2'-fluoro-2'-deoxy-beta-d-5-iodouracil-arabinofuranoside ([(125)i]fiau) to tumors engineered to express the epstein-barr virus thymidine kinase (ebv-tk). here we extend those results to targeting of a therapeutic radiopharmaceutical [(131)i]fiau to slow or stop tumor growth or to achieve t ... | 2008 | 18776891 |
| a mechanism for the hla-a*01-associated risk for ebv+ hodgkin lymphoma and infectious mononucleosis. | | 2008 | 18779413 |
| lymphocyte subset reconstitution patterns in children with small bowel transplantation induced with steroid-free rabbit anti-human thymocyte globulin. | multiple measurements (n = 212) of lymphocyte subsets in 67 children treated with steroid-free tacrolimus, and rabbit anti-human thymocyte globulin induction demonstrate early reconstitution of t-cytotoxic and nk cells. reconstitution of cd4+ cells is complete after the second post-transplant year. during the period at risk for rejection, nk and t-cytotoxic cell counts are significantly higher among rejectors. during periods at increased risk for ebv viral infection, cd4 counts bear a significan ... | 2009 | 18785909 |
| pediatric and young adult nasopharyngeal carcinoma patients treated with preradiation cisplatin and docetaxel chemotherapy. | to evaluate treatment results for pediatric and young adult (aged <21 years) patients with nonmetastatic nasopharyngeal carcinoma treated with neoadjuvant cisplatin + docetaxel and radiotherapy. | 2009 | 18786778 |
| a 13-year-old girl with cold agglutinin syndrome caused by anti-i. | summary: a 13-year-old girl with cold agglutinin syndrome caused by anti-i was serologically positive for epstein-barr virus. the anti-i had a high titer at 4 degrees c and high thermal amplitude (reacting up to 37 degrees c with both cord i rbcs and the patient's autologous rbcs). the patient's hemoglobin dropped to 48 g/l. the age of the patient, the severity of the hemolysis, and the antibody specificity were unusual features of cold agglutinin syndrome. transfusions with adult (i) red blood ... | 2008 | 18797203 |
| kinetics of epstein-barr virus dna load in different blood compartments of pediatric recipients of t-cell-depleted hla-haploidentical stem cell transplantation. | epstein-barr virus (ebv) dna levels in whole-blood samples of 54 pediatric patients receiving t-cell-depleted haploidentical hematopoietic stem cell transplantation (hsct) in 2003 to 2007 were retrospectively compared with ebv dna loads in peripheral blood mononuclear cells (pbmc). determination of ebv dna in whole blood missed 1 of 19 patients (5.2%), who tested positive for ebv dna in pbmc. the analytical sensitivity of ebv dna detection in whole-blood samples relative to that in pbmc was 94.7 ... | 2008 | 18799701 |
| ebv-associated diffuse large b-cell lymphoma arising in the chest wall with surgical mesh implant. | in japan, most cases of malignant lymphoma arising in the thorax are pyothorax-associated lymphoma, which develops in patients who have undergone artificial pneumothorax, used in the past as surgical therapy for pulmonary tuberculosis. pyothorax-associated lymphoma consist mostly of diffuse large b-cell lymphoma and have a strong association with ebv. herein is reported the case of a diffuse large b-cell lymphoma arising in the left thoracic wall after left lung resection for squamous cell carci ... | 2008 | 18801089 |
| the n-terminal domain of ebna1 acts as a suppressor of the her2/neu oncogene. | her2/neu oncogene-mediated malignancy is clearly associated with various human cancers. therefore, her2/neu targeting is an effective approach to cancer therapy. we have previously demonstrated that epstein-barr virus nuclear antigen-1 (ebna1) can suppress her2/neu oncogene expression, although ebna1 itself has oncogenic potential. here, we found that the n-terminal domain of ebna1 alone, named ebna1-nt, which contains the n-terminal region of amino acid residues 1-86 of ebna1, is required and s ... | 2009 | 18805633 |
| expression of egfr and follicular dendritic markers in lymphoid follicles from patients with castleman's disease. | this study investigated the useful morphologic and immunophenotypic findings for the diagnosis of castleman's disease (cd). we focused on the distribution and expression of follicular dendritic cells (fdc) in lymphoid follicles from patients with cd. eleven cd cases of the hyaline vascular (hv) variant and six cases of the plasma cell (pc) variant were studied using tissue microarray and paraffin resistant monoclonal antibodies cd21, cd35, and egfr, a new novel marker of fdc, as well as an antib ... | 2008 | 18813826 |
| epstein-barr virus encephalitis mimicking clinical and electroencephalographic characteristics of herpes simplex encephalitis. | a rare case of ebv encephalitis initially diagnosed as herpes simplex infection is presented to highlight the importance of ebv specific intrathecal elisa and liquor pcr based differential diagnosis when herpes simplex encephalitis specific clinical symptoms, neuroimaging signs and electroencephalographic features are present. the case report also suggests that acyclovir treatment might be beneficial for the long term outcome in adult ebv encephalitis patients. | 2008 | 18814244 |
| points of recombination in epstein-barr virus (ebv) strain p3hr-1-derived heterogeneous dna as indexes to ebv dna recombinogenic events in vivo. | deletions and rearrangements in the genome of epstein-barr virus (ebv) strain p3hr-1 generate subgenomic infectious particles that, unlike defective interfering particles in other viral systems, enhance rather than restrict ebv replication in vitro. reports of comparable heterogeneous (het) dna in ebv-linked human diseases, based on detection of an abnormal juxtaposition of ebv dna fragments bamhi w and bamhi z that disrupts viral latency, prompted us to determine at the nucleotide level all rem ... | 2008 | 18818321 |
| infection and cervical neoplasia: facts and fiction. | whilst there is strong evidence that human papillomavirus (hpv) is the principal aetiological agent in cervical neoplasia, some other sexually transmitted agents may either contribute or protect against cervical carcinogenesis, such as the herpes virus family (hsv), cytomegalovirus (cmv), epstein-barr virus (ebv), human immunodeficiency virus (hiv) or chlamydia trachomatis (ct). epidemiological studies suggest that hsv may have a role in cervical neoplasia, but there is no clear supportive exper ... | 2009 | 18830380 |
| epstein-barr virus-associated b-cell lymphoma secondary to fcd-c therapy in patients with peripheral t-cell lymphoma. | epstein-barr virus (ebv)-associated b-cell lymphoproliferative disorders occur at an increasing frequency in various hereditary and acquired states of immune dysfunction. in a few cases of t-cell lymphoma, especially in angioimmunoblastic t-cell lymphoma (ailt), ebv-associated b-cell lymphoproliferative disorders have been reported. here, we present two cases of ebv-associated b-cell lymphoma after treatment of t-cell lymphoma (ailt and peripheral t-cell lymphoma, unspecified, ptcl-nos) with a r ... | 2008 | 18839273 |
| [diagnosis and management in parotid lymphoepithelioma-like carcinoma]. | to study the diagnosis and treatment of parotid lymphoepithelioma-like carcinoma (lelc), and improve the rate of the diagnosis and treatment. | 2008 | 18841797 |
| [relationship between epstein-barr virus and breast tumors]. | | 2008 | 18844039 |
| histological characteristics of 21 papua new guinean children with high-grade b-cell lymphoma, which is frequently associated with ebv infection. | the aim of the present study was to confirm the histopathological features of aggressive b-cell lymphoma in papua new guinea (png)-an ebv endemic region. the immunophenotypic features and expression of ebv-encoded proteins and rna in b-cell lymphomas were analyzed in 21 png children, and compared to the corresponding features of 17 japanese children with burkitt lymphoma (bl). histological diagnosis of the lymphomas from the png children was bl in nine patients; atypical burkitt/burkitt-like var ... | 2008 | 18844934 |
| cd34+ cord blood cell-transplanted rag2-/- gamma(c)-/- mice as a model for epstein-barr virus infection. | recent studies suggest that epstein-barr virus (ebv) can infect naïve b cells, driving them to differentiate into resting memory b cells via the germinal center reaction. this hypothesis has been inferred from parallels with the biology of normal b cells but has never been proven experimentally. rag2(-/-) gamma(c)(-/-) mice that were transplanted with human cd34(+) cord blood cells as newborns were recently shown to develop human b, t, and dendritic cells, constituting lymphoid organs in situ. h ... | 2008 | 18845836 |
| [correlation of epstein-barr virus-encoded latent membrane protein 1 (lmp1) to fascin and phosphorylated stat3 in nasopharyngeal carcinoma]. | latent membrane protein 1 (lmp1) of epstein-barr (eb) virus is an important oncogene. fascin is an actin cross-linking protein involved in cell migration and adhesion. phosphorylated signal transducer and activator of transcription 3 (pstat3) is a member of stats family, which is closely related to tumorigenesis. this study was to investigate expressions of lmp1, fascin and pstat3 in nasopharyngeal carcinoma (npc) tissues and lymph node metastases of npc, thus to explore their correlations to th ... | 2008 | 18851787 |
| epstein-barr virus associated large b-cell cardiac lymphoma with simultaneous manifestation in the forearm. | | 2008 | 18854558 |
| analysis of bart7 microrna from epstein-barr virus-infected nasopharyngeal carcinoma cells by capillary electrophoresis. | micrornas (mirnas) are a class of approximately 22-nucleotide noncoding rna molecules that negatively regulate their target genes in a sequence-specific manner. in the present study, a fluorescence-labeled antisense dna oligonucleotide was directly hybridized with bart7 mirna in ssc buffered-cetyltrimethylammonium bromide (ctab), followed by capillary electrophoresis with laser-induced fluorescence. the ctab-mediated hybridization allows the probe to anneal the target at 50.0 degrees c, which is ... | 2008 | 18922020 |
| fatal fast-evolution of nasopharyngeal squamous cell carcinoma in an hiv patient with ebv and hpv (-16 and -33) in blood serum. | our case illustrates the first report of an hiv-infected patient with a nasopharyngeal squamous cell carcinoma with viremia by one epstein-barr virus (ebv) and seropositivity by two high risk oncogenic human papilloma viruses (hpv)-types (hpv-16 and hpv-33), previous to his death. this patient presented a fatal fast-evolution. | 2008 | 18923693 |
| [hodgkin's disease variant of richter's syndrome. two cases and literature review]. | we report the clinical and immunohistological features of two cases of chronic lymphocytic leukaemia (cll) with hodgkin's transformation. these cases occurred in a 70-year-old man with a three-year history of cll and in a 76-year-old man with a few months history of cll. microscopic examination showed the presence of large tumor cells with the morphological and immunophenotypic features of classical hodgkin and reed-sternberg (r-s) cells, in a background of otherwise typical b-cll. the transform ... | 2008 | 18928873 |
| quantitative epstein-barr virus viral load monitoring in pediatric liver transplantation. | the aim of this study was to determine whether the implementation of the quantitative epstein-barr virus polymerase chain reaction (qebv-pcr) test in 2003 decreased the incidence of posttransplant lymphoproliferative disease (ptld) and ptld-related mortality. of the 128 children who underwent liver transplantation between january 1994 and may 2007, 110 (85.9%) survived. patients were divided into pre (1994 to 2002; n = 86) and post (2003 to 2007; n = 42) ebv-pcr groups. there were no between-gro ... | 2008 | 18929796 |
| epstein-barr virus nuclear antigen-1 renders lymphocytes responsive to il-2 but not il-15 for survival. | epstein-barr virus nuclear antigen-1 (ebna-1) is the only latent protein expressed in all virus-associated tumours. it plays a critical role in viral propagation and in the replication, episomal maintenance and partitioning of the viral genome. however, its tumorigenic potential is debated. we have previously shown that lymphocytes from a tumour-prone, ebna-1-expressing, transgenic mouse line show increased responsiveness to interleukin-2 (il-2). it was important to determine whether this proper ... | 2008 | 18931080 |
| carcinoembryonic antigen-specific but not antiviral cd4+ t cell immunity is impaired in pancreatic carcinoma patients. | pancreatic carcinoma is a very aggressive disease with dismal prognosis. although evidences for tumor-specific t cell immunity exist, factors related to tumor microenvironment and the presence of immunosuppressive cytokines in patients' sera have been related to its aggressive behavior. carcinoembryonic ag (cea) is overexpressed in 80-90% of pancreatic carcinomas and contains epitopes recognized by cd4(+) t cells. the aim of this study was to evaluate the extent of cancer-immune surveillance and ... | 2008 | 18941250 |