| influence of seasonality on the genetic diversity of vibrio parahaemolyticus in new hampshire shellfish waters as determined by multilocus sequence analysis. | risk of gastric infection with vibrio parahaemolyticus increases with favorable environmental conditions and population shifts that increase prevalence of infective strains. genetic analysis of new hampshire strains revealed a unique population with some isolates similar to outbreak-causing strains and high-level diversity that increased as waters warmed. | 2012 | 22407686 |
| the vibrio parahaemolyticus toxrs regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model. | vibrio parahaemolyticus, a marine bacterium, is the causative agent of gastroenteritis associated with the consumption of seafood. it contains a homologue of the toxrs operon that in v. cholerae is the key regulator of virulence gene expression. we examined a nonpolar mutation in toxrs to determine the role of these genes in v. parahaemolyticus rimd2210633, an o3:k6 isolate, and showed that compared to the wild type, δtoxrs was significantly more sensitive to acid, bile salts, and sodium dodecyl ... | 2012 | 22392925 |
| structural insights into genetic variants of na(+)/glucose cotransporter sglt1 causing glucose-galactose malabsorption: vsglt as a model structure. | current advances in structural biology provide valuable insights into structure-function relationship of membrane transporters by solving crystal structures of bacterial homologs of human transporters. therefore, scientists consider bacterial transporters as useful structural models for designing of drugs targeted in human diseases. the functional homology between vibrio parahaemolyticus na(+)/galactose transporter (vsglt) and na(+)/glucose cotransporter sglt1 has been well established a decade ... | 2012 | 22383112 |
| detection of vibrio parahaemolyticus in shellfish using polymerase chain reaction-enzyme-linked immunosorbent assay. | this study evaluated the application of polymerase chain reaction-enzyme-linked immunosorbent assay (pcr-elisa) for the detection of vibrio parahaemolyticus in shellfish. | 2012 | 22380509 |
| cloning and expression analysis of interferon regulatory factor (irf) 3 and 7 in large yellow croaker, larimichthys crocea. | the interferon regulatory factor (irf)3 and irf7 are considered to play essential roles in innate immune system's antiviral responses. in this report, the full-length cdna and genomic structure and immune response characterizations of irf3 and irf7 were investigated in large yellow croaker, larimichthys crocea. the full-length cdna of l. crocea (lc)irf3 was of 2204 bp, including a 5'-terminal untranslated region (utr) of 41 bp, a 3'-terminal utr of 774 bp and an open reading frame (orf) of 1389 ... | 2012 | 22374413 |
| dna array with the groesl intergenic sequence to detect vibrio parahaemolyticus and vibrio vulnificus. | the untranscribed dna sequences of the intergenic spacer regions (isrs) in the groesl gene of 23 vibrio species were determined and compared. isr sequence length (41-85 bp) was variable. vibrio species could be divided into three groups according to the length and homology of their isr sequences. dna array hybridization using isr-specific probes accurately distinguished vibrio parahaemolyticus and vibrio vulnificus from other species. | 2012 | 22342881 |
| [comparative study on the phenotypic characteristics and molecular typing of foodborne vibrio parahaemolyticus in guangdong province]. | to understand the phenotypic characteristics of foodborne vibrio parahaemolyticus in guangdong province through carrying out a comprehensive comparison including pulse field gel electrophoresis, ribotyping and serotyping. | 2011 | 22336613 |
| [analysis on the etiological and molecular epidemiological characteristics of vibrio parahaemolyticus in beijing]. | to understand the etiological and molecular-epidemiological characteristics of vibrio parahaemolyticus in beijing. | 2011 | 22336612 |
| [etiologic characteristics of vibrio parahaemolyticus strains causing outbreaks and sporadic cases in guangdong, 2009]. | to study the serotypes, virulence features and molecular characteristics of vibrio parahaemolyticus isolated in food poisoning cases and surveillance program on diarrhea patients in guangdong, 2009. | 2011 | 22336608 |
| vibrio parahaemolyticus in rhode island coastal ponds and the estuarine environment of narragansett bay. | quantification of the abundance of vibrio parahaemolyticus in water and oysters from rhode island showed the presence of environmental strains and low levels of potentially pathogenic strains when water temperatures were ≥18°c, with peak levels in late july to early august. a higher abundance of the trh gene than of the tdh gene was observed. | 2012 | 22307298 |
| microevolution of pandemic vibrio parahaemolyticus assessed by the number of repeat units in short sequence tandem repeat regions. | the emergence of the pandemic strain vibrio parahaemolyticus o3:k6 in 1996 caused a large increase of diarrhea outbreaks related to seafood consumption in southeast asia, and later worldwide. isolates of this strain constitutes a clonal complex, and their effectual differentiation is possible by comparison of their variable number tandem repeats (vntrs). the differentiation of the isolates by the differences in vntrs will allow inferring the population dynamics and microevolution of this strain ... | 2012 | 22292049 |
| characterization of low salinity stress in vibrio parahaemolyticus. | vibrio parahaemolyticus is a marine foodborne pathogenic bacterium commonly found in seawater or seafood. this bacterium often encounters low salinity stress when the contaminated seafood is washed with fresh water during food processing. this study was conducted to investigate the response of exponential- and stationary-phase cells of v. parahaemolyticus st550 to lethal or sublethal low salinity. tolerance to lethal low salinity (0.25% nacl) was enhanced in v. parahaemolyticus cells in the expo ... | 2012 | 22289582 |
| association of pandemic vibrio parahaemolyticus o3:k6 present in the coastal environment of northwest mexico with cases of recurrent diarrhea between 2004 and 2010. | in 2004, more than 1,230 cases of gastroenteritis due to pandemic o3:k6 strains of vibrio parahaemolyticus were reported in southern sinaloa, a state in northwestern mexico. recurrent sporadic cases arose from 2004 to 2010, spreading from the south to the north. in the present study, vibrio parahaemolyticus was detected in both environmental samples and clinical cases along the pacific coast of sinaloa during 2004 to 2010. an evaluation was made of the serotypes, distribution of virulence genes, ... | 2012 | 22247160 |
| molecular typing of vibrio parahaemolyticus isolates from the middle-east coastline of china. | the occurrence of outbreaks of vibrio parahaemolyticus gastroenteritis in china highlights the need for strain characterization and subtyping of this pathogenic species. a total of 56 epidemiologically-unrelated strains of v. parahaemolyticus were isolated from clinical samples, seafood and various environmental sites in the middle-east coastline of china from 2006 to 2008. the isolates were characterized using four molecular typing methods, including ribotyping, enterobacterial repetitive inter ... | 2012 | 22225982 |
| evaluation of most-probable-number-pcr method with internal amplification control for the counting of total and pathogenic vibrio parahaemolyticus in frozen shrimps. | the most-probable-number (mpn) method is often time-consuming for the isolation, detection, and quantification of vibrio parahaemolyticus from natural sources. mpn counting of v. parahaemolyticus bacteria usually involves the isolation of typical v. parahaemolyticus colonies on selective medium, with subsequent confirmation by biochemical identification. in this study, we evaluated the use of a pcr on mpn enrichment cultures (mpn-pcr) for the direct detection of total and pathogenic v. parahaemo ... | 2012 | 22221369 |
| isolation, molecular characterization, and antibiotic susceptibility of vibrio parahaemolyticus in korean seafood. | the principal objective of this study was to investigate the incidence, risk assessment, antibiotic resistance, and genotyping of vibrio parahaemolyticus in korean seafood. the incidence of v. parahaemolyticus in seafood obtained from several fish markets in korea was investigated from may to december of 2009, except between july and september. two selective mediums (tcbs [thiosulfate, citrate, bile salts, and sucrose] agar and chromagar™ vibrio) were used, and the v. parahaemolyticus strains we ... | 2012 | 22216989 |
| molecular cloning, sequence analysis and expression of fein-penaeidin from the haemocytes of indian white shrimp fenneropenaeus indicus. | penaeidins are members of a special family of antimicrobial peptide existing in penaeid shrimp and play an important role in the immunological defense of shrimp. here, we report a penaeidin sequence cloned from the indian white shrimp fenneropenaus indicus (fein-penaeidin). the fein-penaeidin open reading frame encodes a 77 amino acid peptide including a 19 amino acid signal peptide. the deduced amino acid sequences of fein-penaeidin include a proline rich n-terminal domain and a carboxyl-domain ... | 2012 | 24371565 |
| antibiotic resistance of vibrio parahaemolyticus isolated from pond-reared litopenaeus vannamei marketed in natal, brazil. | ten out of fifty fresh and refrigerated samples of shrimp (litopenaeus vannamei) collected from retailers in natal (rio grande do norte, northeastern brazil) tested positive for vibrio parahaemolyticus. the kanagawa test and multiplex pcr assays were used to detect tdh and trh hemolysins and the tdh, trh and tlh genes, respectively. all strains were kanagawa-negative and tlh-positive. antibiotic susceptibility testing was done for seven antibiotics by the agar diffusion technique. five strains ( ... | 2011 | 24031779 |
| prevalence, serotyping and molecular characterization of vibrio parahaemolyticus in mussels from italian growing areas, adriatic sea. | the aims of this study were to investigate the prevalence of total and pathogenic vibrio parahaemolyticus strains in italian mussels from different geographical areas of the adriatic sea and to determine their serotypes, toxigenic profiles and pandemic potential. out of 559 samples analysed during 2007, 65 (11.6%) were positive for v. parahaemolyticus. none of the isolates had the genes for thermostable direct haemolysin (tdh) and pandemic marker (toxrs), while five strains (7.7%) had that for t ... | 2010 | 23766016 |
| detection of environmental vibrio parahaemolyticus using a polyclonal antibody by flow cytometry. | the aim of this study was to detect and quantify vibrio parahaemolyticus using flow cytometry (fcm) in combination with a polyclonal antibody developed in our laboratory. experiments were carried out using v. parahaemolyticus cells in pure and mixed bacteria culture suspensions in either artificial or natural seawater. using fcm, v. parahaemolyticus cells labelled with the polyclonal antibody and a secondary fluorescein isothiocyanate-conjugated antibody were detected and rapidly quantified at l ... | 2010 | 23766012 |
| serodiversity and ecological distribution of vibrio parahaemolyticus in the venetian lagoon, northeast italy. | vibrio parahaemolyticus is a natural inhabitant of estuarine and marine environments constituting part of the autochthonous microflora. this species is associated with human gastroenteritis caused by ingestion of contaminated water and undercooked seafood. during the past several years, the number of v. parahaemolyticus gastroenteritis cases have increased worldwide, causing over half of all food-poisoning outbreaks of bacterial origin. vibrio populations in water are known to be influenced by e ... | 2010 | 23766011 |
| prevalence and genetic diversity of pathogenic populations of vibrio parahaemolyticus in coastal waters of galicia, spain. | the natural reservoirs and biological characteristics of pathogenic populations of vibrio parahaemolyticus in marine habitats remain unclear due to difficulties in obtaining pathogenic strains from the environment. the distribution and characteristics of pathogenic v. parahaemolyticus were investigated over 1 year in three coastal environments in galicia (spain), including areas of the major international ports in the region. vibrio parahaemolyticus was present in 35.3% of the samples analysed, ... | 2010 | 23765999 |
| environmental occurrence and clinical impact of vibrio vulnificus and vibrio parahaemolyticus: a european perspective. | vibrio vulnificus and vibrio parahaemolyticus are ubiquitous gram-negative bacterial pathogens found naturally in marine and estuarine waters, and are a leading cause of seafood-associated bacterial illness. these pathogens are commonly reported in the usa and in many asian countries, including china, japan and taiwan; however, there is growing concern that v. vulnificus and v. parahaemolyticus may represent an important and increasing clinical problem in europe. several factors underlie the nee ... | 2010 | 23765993 |
| production of new restriction endonuclease vpak111bi recognizing sequence 5'-ggwcc-3' in a haemolysin-less mutant of vibrio parahaemolyticus. | high restriction endonuclease activity was found in a haemolysin-less mutant of the vibrio parahaemolyticus 1743-1 strain. the endonuclease, named vpak11bi, recognized the palindromic pentanucleotide sequence of 5'-ggwcc-3' and cleaved double-stranded dna after the first g, which is exactly the same as the specificity of avaii. the haemolysin-less mutant of v. parahaemolyticus is now available for producing the valuable restriction endonuclease on a commercial scale. | 1997 | 27396891 |
| isolation and characterization of a toxic intracellular protein from vibrio parahaemolyticus. | a toxic factor released from disrupted cells of vibrio parahaemolyticus was partially purified by gel filtration after precipitation with (nh4)2so4 at 40% saturation. the factor, which was a thermostable protein of 63 kda, lysed human erythrocytes at a concentration of 0.15 μg ml(-1). its ld50 by intravenous injection into mice was 6.4 μg. fluid accumulated in suckling mice force-fed with the toxic material (1 to 25 μg). haemolytic activity, which occurred maximall at 37°c and ph 7.0 was enhance ... | 1996 | 24415088 |
| vibrio parahaemolyticus andvibrio alginolyticus: short generation-time marine bacteria. | the growth rates of 30 different strains ofvibrio parahaemolyticus andvibrio alginolyticus at 37°c was determined. each species consists of two major groups, one having a short generation time (12-14 min) and one with a longer generation time (20-25 min). the diversity in generation times of different strains belonging to the same species is discussed. the effect of temperature, salt, and nutrient concentrations on the growth rate of onev. alginolyticus strain (ncmb 1803) was studied. the most s ... | 1974 | 24241024 |
| antimicrobial polyketide furanoterpenoids from seaweed-associated heterotrophic bacterium bacillus subtilis mtcc 10403. | brown seaweed anthophycus longifolius (turner) kützing (family sargassaceae) associated heterotrophic bacterium bacillus subtilis mtcc 10403 was found to be a potent isolate with broad range of antibacterial activity against important perceptive food pathogens vibrio parahaemolyticus, v. vulnificus, and aeromonas hydrophila. this bacterium was positive for polyketide synthetase gene (kc589397), and therefore, was selected to bioprospect specialized metabolites bearing polyketide backbone. bioact ... | 2017 | 28704687 |
| antibacterial and antioxidant constituents of extracts of endophytic fungi isolated from ocimum basilicum var. thyrsiflora leaves. | fourteen fungal endophytes were isolated from the ocimum basilicum var. thyrsiflora leaves collected from northern thailand. eight genera were identified including aspergillus, ascochyta, nigrospora, blastomyces, colletotrichum, exidia, clitopilus, and nomuraea. the antibacterial activity of crude extracts from all endophytic fungi was tested against nine human bacterial pathogens: staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, bacillus cereus, escherichia coli, klebsiella ... | 2017 | 28698913 |
| identification and growth optimization of a marine bacillus dk1-sa11 having potential of producing broad spectrum antimicrobial compounds. | control of harmful bacteria in food, aquaculture, pharmaceuticals, agriculture, hospitals and recreation water pools are of great global concern. marine bacteria are an enormous source of bio-controlling agents. the aim of this study was to identify and optimize the growth conditions including effect of different biotic and abiotic factors on antimicrobial activity of strain dk1-sa11 isolated from qingdao bay of china yellow sea. microscopic characterization, api® 20e and 50 chb kit base carbohy ... | 2017 | 28653930 |
| antibacterial and antifungal activities of spices. | infectious diseases caused by pathogens and food poisoning caused by spoilage microorganisms are threatening human health all over the world. the efficacies of some antimicrobial agents, which are currently used to extend shelf-life and increase the safety of food products in food industry and to inhibit disease-causing microorganisms in medicine, have been weakened by microbial resistance. therefore, new antimicrobial agents that could overcome this resistance need to be discovered. many spices ... | 2017 | 28621716 |
| development of a gold nanoparticle-based universal oligonucleotide microarray for multiplex and low-cost detection of foodborne pathogens. | bacterial foodborne diseases remain major threats to food safety and public health, especially in developing countries. in this study a novel assay, combining gold nanoparticle (gnp)-based multiplex oligonucleotide ligation-pcr and universal oligonucleotide microarray technology, was developed for inexpensive, specific, sensitive, and multiplex detection of eight common foodborne pathogens, including shigella spp., campylobacter jejuni, bacillus cereus, escherichia coli o157:h7, listeria monocyt ... | 2017 | 28505584 |
| seven new lignan glycosides from the branches of alangium kurzii craib var. laxifolium. | seven new lignan glycosides (1-3, 8-10, and 14) and 17 known compounds were isolated from the branches of alangium kurzii craib var. laxifolium. their structures were established by spectroscopic analysis and circular dichroism (cd) and x-ray analysis. the isolated compounds were evaluated for their antibacterial activities against staphylococcus aureus ci1011, streptococcus suis ci1608, salmonella gallinarum ci0912, enterococcus faecalis ci1304, aeromonas hydrophila ci1008, escherichia coli ci1 ... | 2017 | 28733150 |
| preparation, characterization and antibacterial activity of chitosan and phosphorylated chitosan from cuttlebone of sepia kobiensis (hoyle, 1885). | chitosan is a commercially available derivative of chitin that has been extensively studied for its antimicrobial properties. in order to improve the water solubility and its biological activity, the chemical modification or derivatisation is attempted. in the present investigation, the chitosan prepared from the cuttlebone of sepia kobiensis was being chemically modified by reacting it with orthophosphoric acid so as to obtain phosphorylated chitosan. then the chitosan and phosphorylated chitos ... | 2016 | 28507912 |
| identification and characterization of a laccase from litopenaeus vannamei involved in anti-bacterial host defense. | phenoloxidases (pos) are a family of enzymes including tyrosinases, catecholases and laccases, which play an important role in immune defences of various invertebrates. whether or not laccase exists in shrimp and its function is still poorly understood. in this study, a laccase (lvlac) was cloned and identified from litopenaeus vannamei for the first time. the full length of lvlac is 3406 bp, including a 2034 bp open reading frame (orf) coding for a putative protein of 677 amino acids with a sig ... | 2017 | 28476665 |
| identification of a macrobrachium nipponense c-type lectin with a close evolutionary relationship to vertebrate lectins. | c-type lectins (ctls) are involved in the innate immune defense of vertebrates and invertebrates against invading pathogens. this study cloned and characterized a novel c-type lectin (mnctl) of the oriental river prawn, macrobrachium nipponense. the cloned mnctl cdna encompasses an open reading frame of 774 nucleotides and encodes polypeptides of 257 residues. the deduced mnctl protein contains a single carbohydrate recognition domain (crd) with an epn (glu-pro-asn) motif in calcium-binding site ... | 2017 | 28441623 |
| vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) from the adriatic coast of italy. | a case of vibrio parahaemolyticus- and v. alginolyticus-associated meningo-encephalitis in a bottlenose dolphin (tursiops truncatus) found stranded along the adriatic coast of italy in 2016 is herein reported, along with a minireview on v. parahaemolyticus and v. alginolyticus infections in aquatic mammals. macroscopically, two abscesses were found in the dolphin's forebrain, along with an extensive, bilateral, parasitic broncho-pneumonia. histologically, a suppurative-to-pyogranulomatous mening ... | 2017 | 28709108 |
| previously undescribed antibacterial polyketides from heterotrophic bacillus amyloliquefaciens associated with seaweed padina gymnospora. | a heterotrophic marine bacterium bacillus amyloliquefaciens isolated from seaweed padina gymnospora exhibited broad spectra of antibacterial activities against pathogenic bacteria aeromonas hydrophila, vibrio harveyi, vibrio vulnificus, and vibrio parahaemolyticus. the seaweed-associated b. amyloliquefaciens was recognized to possess functional type i polyketide synthase-1 (pks-1) gene, and was used to isolate four homologous compounds with polyketide frameworks. the compounds were characterized ... | 2017 | 28842846 |
| corrigendum: assessing the function of stas domain protein sypa in vibrio fischeri using a comparative analysis. | [this corrects the article on p. 760 in vol. 6, pmid: 26284045.]. | 2017 | 28450861 |
| a single wap domain-containing protein from litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against ahpnd-inducing vibrio parahaemolyticus. | the single wap domain-containing protein (swd) is a type iii crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. a study of swd from litopenaeus vannamei (lvswd) is reported herein about its activities and function against bacteria, particularly the ahpnd-inducing vibrio parahaemolyticus (vpahpnd) that causes acute hepatopancreatic necrosis disease (ahpnd). the lvswd is mainly synthesi ... | 2017 | 28743625 |
| bioluminescence imaging to track bacteroides fragilis inhibition of vibrio parahaemolyticus infection in mice. | bacteroides fragilis is an anaerobic, gram-negative, commensal bacterium of the human gut. it plays an important role in promoting the maturation of the immune system, as well as suppressing abnormal inflammation. many recent studies have focused on the relationship between b. fragilis and human immunity, and indicate that b. fragilis has many useful probiotic effects. as inhibition of intestinal pathogens is an important characteristic of probiotic strains, this study examined whether b. fragil ... | 2017 | 28553617 |
| whole-body microbiota of sea cucumber (apostichopus japonicus) from south korea for improved seafood management. | sea cucumber (apostichopus japonicus) is a popular seafood source in asia, including south korea, and its consumption has recently increased with recognition of its medicinal properties. however, because raw sea cucumber contains various microbes, its ingestion can cause foodborne illness. therefore, analysis of the microbiota in the whole body of sea cucumber can extend our understanding of foodborne illness caused by microorganisms and help to better manage products. we collected 40 sea cucumb ... | 2017 | 28851208 |
| role of purified β-1, 3 glucan binding protein (β-gbp) from paratelphusa hydrodromus and their anti-inflammatory, antioxidant and antibiofilm properties. | β- 1, 3-glucan binding protein (β-gbp), a pattern recognition protein (prp), plays a critical role in triggering the innate immune response by detecting β-glucan found on the surface of microbes. in the present study, β-gbp was purified from the haemolymph of rice field crab paratelphusa hydrodromus by affinity column chromatography. the monomeric protein ph-β-gbp appeared as a single band with a molecular weight of approximately 95 kda in sds-page analysis and its purity was determined to be 89 ... | 2017 | 28684323 |
| expression of a vibrio parahaemolyticus toxin in escherichia coli results in chromosomal dna degradation. | a toxin-antitoxin system, vp1842/vp1843, locates within a superintegron on the vibrio parahaemolyticus genome chromosome i whose toxin gene vp1843 encodes a dna nicking endonuclease. we found that the vp1843 expression in escherichia coli cells strongly induced chromosomal dna degradation. on the basis of these observations, we discuss a possible physiological role of vp1842/vp1843 in v. parahaemolyticus. | 2017 | 28820009 |
| ecofriendly synthesis of silver and gold nanoparticles by euphrasia officinalis leaf extract and its biomedical applications. | biogenic synthesis of silver (agnps) and gold nanoparticles (aunps) using aqueous extract of euphrasia officinalis has been reported. stable agnps and aunps were formed on adding aqueous solutions of silver nitrate and chloroauric acid with e. officinalis leaf extract, in 19 min and 2 min, respectively. the synthesis method used in present study was simple, reliable, rapid, cost effective and ecofriendly. the synthesized nanoparticles were characterized with field emission transmission electron ... | 2017 | 28784039 |
| seasonal influence of scallop culture on nutrient flux, bacterial pathogens and bacterioplankton diversity across estuaries off the bohai sea coast of northern china. | in this study, we investigated the environmental impacts of scallop culture on two coastal estuaries adjacent the bohai sea including developing a quantitative pcr assay to assess the abundance of the bacterial pathogens escherichia coli and vibrio parahaemolyticus. scallop culture resulted in a significant reduction of nitrogen, chlorophyll a, and phosphorous levels in seawater during summer. the abundance of bacteria including v. parahaemolyticus varied significantly across estuaries and breed ... | 2017 | 28779889 |
| removal of foodborne pathogen biofilms by acidic electrolyzed water. | biofilms, which are complex microbial communities embedded in the protective extracellular polymeric substances (eps), are difficult to remove in food production facilities. in this study, the use of acidic electrolyzed water (aew) to remove foodborne pathogen biofilms was evaluated. we used a green fluorescent protein-tagged escherichia coli for monitoring the efficiency of aew for removing biofilms, where under the optimal treatment conditions, the fluorescent signal of cells in the biofilm di ... | 2017 | 28638370 |
| a rele/pare superfamily toxin in vibrio parahaemolyticus has dna nicking endonuclease activity. | type ii toxins in toxin-antitoxin (ta) systems fold into a similar fold and belong to the rele/pare superfamily. however, they display two distinct biochemical activities: rele toxins are mrna interferases, while pare toxins are dna gyrase (gyr) inhibitors. previously, we found a ta system, vp1842/vp1843, on the vibrio parahaemolyticus genome whose toxin vp1843 belongs to the rele/pare toxin superfamily. vp1843, unlike rele toxins, has neither protein synthesis inhibitory activity nor ribonuclea ... | 2017 | 28533087 |
| molecular characteristics, biofilm-forming abilities, and quorum sensing molecules in vibrio parahaemolyticus strains isolated from marine and clinical environments in korea. | vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. in this study, an ultrafast labchip real-time pcr assay was evaluated for rapid detection and quantification of pathogenic v. parahaemolyticus isolates. escherichia coli and vibrio harveyi were used as negative controls. twenty-six tdh-positive, biofilm-producing v. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction ... | 2017 | 28452235 |
| application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review. | electrolysed oxidising water (e.o. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. at neutral ph this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. e.o. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. the efficacy of e.o. water against pathogenic ba ... | 2017 | 28416883 |
| groel is a suitable genetic marker for detecting vibrio parahaemolyticus by loop-mediated isothermal amplification assay. | a groel gene-based loop-mediated isothermal amplification (lamp) assay was developed to detect vibrio parahaemolyticus in contaminated seafood and water. the assay was optimized and conducted at 63°c for 40 min using bacillus stearothermophilus (bst) dna polymerase, large fragment. amplification was analysed via multiple detection methods, including opacity, formation of white precipitate, dna intercalating dyes (ethidium bromide and sybr green i), metal ion-binding indicator dye, calcein, and 2 ... | 2017 | 28585379 |
| purification and antibacterial mechanism of fish-borne bacteriocin and its application in shrimp (penaeus vannamei) for inhibiting vibrio parahaemolyticus. | vibrio parahaemolyticus: is recognized as the main cause of gastroenteritis associated with consumption of seafood. bacteriocin-producing lactobacillus plantarum fgc-12 isolated from golden carp intestine had strong antibacterial activity toward v. parahaemolyticus. the fish-borne bacteriocin was purified by a three-step procedure consisting of ethyl acetate extraction, gel filtration chromatography and high performance liquid chromatography. its molecular weight was estimated at 4.1 kda using s ... | 2017 | 28702797 |
| molecular identification of disk abalone (haliotis discus discus) tetraspanin 33 and cd63: insights into potent players in the disk abalone host defense system. | tetraspanins are a superfamily of transmembrane proteins involved in a diverse range of physiological processes including differentiation, adhesion, signal transduction, cell motility, and immune responses. in the present study, two tetraspanins, cd63 and tetraspanin 33 (tspan33) from disk abalone (abcd63 and abtspan33), were identified and characterized at the molecular level. the coding sequences for abcd63 and abtspan33 encoded polypeptides of 234 and 290 amino acids (aa) with predicted molec ... | 2017 | 28823981 |
| a cysteine protease (cathepsin z) from disk abalone, haliotis discus discus: genomic characterization and transcriptional profiling during bacterial infections. | cathepsin z (ctsz) is lysosomal cysteine protease of the papain superfamily. it participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. hence, ctsz is also acknowledged as an acute-phase protein in host immunity. in this study, we sought to identify the ctsz homolog from disk abalone (abctsz) and characterize it at the molecular, genomic, and transcrip ... | 2017 | 28687334 |
| new chlorinated xanthone and anthraquinone produced by a mangrove-derived fungus penicillium citrinum hl-5126. | two new chlorinated metabolites 4-chloro-1-hydroxy-3-methoxy-6-methyl-8-methoxycarbonyl-xanthen-9-one (1) and 2'-acetoxy-7-chlorocitreorosein (2), together with three known compounds (3-5), were obtained from the etoac extract of the endophytic fungus penicillium citrinum hl-5126 isolated from the mangrove bruguiera sexangula var. rhynchopetala collected in the south china sea. their structures were elucidated by the detailed analysis of comprehensive spectroscopic data. all compounds were evalu ... | 2017 | 28465625 |
| antibacterial activities and preservative effect of chitosan oligosaccharide maillard reaction products on penaeus vannamei. | the purpose of this study was to understand the influence of maillard reaction (mr) on bioactivity of chitosan oligosaccharide (cos). the antibacterial activity against pseudomonas aeruginosa and vibrio parahaemolyticus and preservative effect of two kinds of cos mr products (mrps) on penaeus vannamei were evaluated. the structures of cg (mrps of cos and glucose) and cm (mrps of cos and maltose) were characterized by ft-ir and their molecular weights were measured by gel permeation chromatograph ... | 2017 | 28732733 |
| a c1qdc protein (hcc1qdc6) with three tandem c1q domains is involved in immune response of triangle-shell pearl mussel (hyriopsis cumingii). | c1q-domain-containing (c1qdc) proteins are a family of proteins with a globular c1q (gc1q) domain and participate in several immune responses. in this study, a c1qdc gene was identified from the triangle-shell pearl mussel hyriopsis cumingii (designated as hcc1qdc6). this gene has a full-length cdna of 1782 bp and an open reading frame of 1,335 bp that encodes a 444-amino acid polypeptide containing three gc1q domains. hcc1qdc6 contains at least five exons and four introns. the mrna transcripts ... | 2017 | 28785225 |
| microencapsulation of essential thyme oil by spray drying and its antimicrobial evaluation against vibrio alginolyticus and vibrio parahaemolyticus. | the aims of this research were first, to evaluate the antibacterial potential of commercial thyme essential oil against v. alginolyticus and v. parahaemolyticys and second, using the spray drying technique to produce microcapsules. chemical compounds of thyme oil and microcapsules were identified and quantified being thymol the chemical component present at the highest concentration. oil-in-water (o/w) emulsions were prepared and the microcapsules were obtained with a spray dryer using maltodext ... | 2017 | 28832836 |
| occurrence of vibrio species, beta-lactam resistant vibrio species, and indicator bacteria in ballast and port waters of a tropical harbor. | ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. ballast waters from six ships docked in the port of singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation d-2 of the ballast water management convention (bwmc) guidelines. vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant vibr ... | 2017 | 28822933 |
| molecular cloning, characterization and functional analysis of a putative mitogen-activated protein kinase kinase kinase 4 (mekk4) from blood clam tegillarca granosa. | the mitogen-activated protein kinase (mapk) cascades stand for one of the most important signaling mechanisms in response to environmental stimuli. in the present study, we cloned and identified for the first time the full-length cdna of mapk kinase kinase 4 (tgmekk4) from blood clam tegillarca granosa using rapid amplification of cdna ends method. the full-length cdna of tgmekk4 was of 1605 bp in length, encoding a polypeptide of 364 amino acids with a predicted molecular mass of 41.22 kda and ... | 2017 | 28476674 |
| environmental vibrios represent a source of antagonistic compounds that inhibit pathogenic vibrio cholerae and vibrio parahaemolyticus strains. | with the overuse of antibiotics, many pathogens including vibrio cholerae and vibrio parahaemolyticus have evolved multidrug resistance making treatment more difficult. while understanding the mechanisms that underlie pathogenesis is crucial, knowledge of bacterial interactions of v. cholerae and v. parahaemolyticus could provide insight to their susceptibility outside of the human host. based on previous work showing competition among environmental strains, we predict that marine-derived bacter ... | 2017 | 28857444 |
| effects of desiccation practices of cultured atlantic oysters (crassostrea virginica) on vibrio spp. in portersville bay, alabama, usa. | the expansion of off-bottom aquaculture to the gulf of mexico has raised public health concerns for human health officials. high temperatures in the gulf of mexico are associated with high levels of vibrio parahaemolyticus and vibrio vulnificus. routine desiccation practices associated with off-bottom aquaculture expose oysters to ambient air, allowing vibrio spp. to proliferate in the closed oyster. currently, there is limited research on the length of time needed for vibrio spp. levels in desi ... | 2017 | 28696147 |
| a double-quadratic model for predicting vibrio species in water environments of japan. | vibrio spp. are natural inhabitants of marine and estuarine environments. vibrio cholerae, vibrio parahaemolyticus, and vibrio vulnificus are the major infectious agents for humans. their densities are affected by environmental factors such as water temperature and salinity. the detailed contribution of each factor still remains to be elucidated. here we conducted multi-coastal study in a 21-month period to examine relationships between environmental factors and v. cholerae, v. parahaemolyticus ... | 2017 | 28646477 |
| genome analysis of three novel lytic vibrio coralliilyticus phages isolated from seawater, okinawa, japan. | three novel vibrio phages were isolated from seawater in okinawa. the vibrio phage ryc infected vibrio coralliilyticus swa 07, while vibrio phages ckb-s1 and ckb-s2 infected the coral pathogen v. coralliilyticus p1 (lmg 23696). the vibrio phages ckb-s1 and ckb-s2 displayed head-tail structures whereas the vibrio phage ryc showed a tailless non-enveloped capsid. all these vibrio phages contained linear and double-stranded dna. the whole genome sequencing revealed that vibrio phage ryc has a large ... | 2017 | 28689690 |
| antagonistic activity of thymus vulgaris extracts against vibrio species isolated from seafoods. | this study examined the antibacterial activity of thymus vulgaris on multiple antibiotic resistant (mar) vibrio parahaemolyticus and vibrio fluvialis isolated from shrimps. the ethanol extract of t. vulgaris antibacterial properties was assessed using the agar diffusion method. survival of test organisms in shrimp meat using different concentrations of t. vulgaris was done using standard method. the quantitative and qualitative phytochemical tests of t. vulgaris extract were determined. the etha ... | 2017 | 28416870 |
| environmental determinants of vibrio parahaemolyticus in the chesapeake bay. | vibrio parahaemolyticus naturally-occurs in brackish and marine waters and is one of the leading causes of seafood-borne illness. previous work studying the ecology of v. parahaemolyticus is often limited in geographic extent and lacking a full range of environmental measures. this study used a unique, large dataset of surface water samples in the chesapeake bay (n=1,385) collected from 148 monitoring stations from 2007 to 2010. water was analyzed for over 20 environmental parameters with additi ... | 2017 | 28842541 |
| variation of genomic islands and flanking fragments in vibrio parahaemolyticus isolates from environmental and clinical sources in taiwan. | vibrio parahaemolyticus is a halophilic foodborne pathogenic bacterium that causes gastroenteritis; it has become an issue of global concern since the emergence and spread of pandemic o3:k6 strains. this study evaluated the role of vibrio pathogenicity island (vpai)-associated fragments in the genetic variation and grouping of this pathogen. distribution of some vpai fragments and flanking fragments (vpai-1, vpai-4, vpai-5, vpai-6 and vpai-7) was determined in a total of 53 v. parahaemolyticus i ... | 2017 | 28841447 |
| inactivation of vibrio parahaemolyticus in shucked raw oyster ( grassostrea gigas) and clam ( venerupis phillippinarum) by using a combination of naclo and gamma irradiation. | this study investigated the synergistic effects of sodium hypochlorite (naclo) and gamma irradiation combination against vibrio parahaemolyticus in shucked oysters and clams. v. parahaemolyticus decreased to 1.1-5.6 log10 cfu/g in oysters and 1.1-5.7 log10cfu/g in clams by naclo (20-80 ppm) + γ irradiation (0.1-2.0 kgy) combinations. v. parahaemolyticus was not detected by 60 or 80 ppm naclo + 2.0 kgy. synergistic reduction of >1 log was observed by 60 ppm naclo + 0.3-2 kgy and 80 ppm naclo + 0. ... | 2017 | 28840748 |
| microbiome dynamics in a shrimp grow-out pond with possible outbreak of acute hepatopancreatic necrosis disease. | acute hepatopancreatic necrosis disease (ahpnd) (formerly, early mortality syndrome) is a high-mortality-rate shrimp disease prevalent in shrimp farming areas. although ahpnd is known to be caused by pathogenic vibrio parahaemolyticus hosting the plasmid-related pirab(vp) toxin gene, the effects of disturbances in microbiome have not yet been studied. we took 62 samples from a grow-out pond during an ahpnd developing period from days 23 to 37 after stocking white postlarvae shrimp and sequenced ... | 2017 | 28839269 |
| in vitro isothermal nucleic acid amplification assisted surface-enhanced raman spectroscopic (sers) for ultrasensitive detection of vibrio parahaemolyticus. | detection of pathogenic microorganisms is of great importance for public health and food safety. traditional protocols can hardly meet the continuously increased demanding in sensitivity and specificity of pathogen detections. in this study, we adopted vibrio parahaemolyticus (v. parahaemolyticus, vp) as the model analyte, and developed an antibody-vp-aptamer hetero-sandwich based surface-enhanced raman scattering (sers) method in conjunction with in vitro isothermal amplification for sensitive ... | 2017 | 28832123 |
| proteomic analysis of vibrio parahaemolyticus under cold stress. | vibrio parahaemolyticus is a kind of food-borne pathogenic bacterium, which can seriously infect food, especially seafood causing gastroenteritis and other disease. we studied the global proteome responses of v. parahaemolyticus under cold stress by nano-liquid chromatography-tandem mass spectrometry to improve the present understanding of v. parahaemolyticus proteomics events under cold stress. a total of 1151 proteins were identified and 101 proteins were differentially expressed, of which 69 ... | 2017 | 28831596 |
| characterization, expression, and evolutionary analysis of new tlr3 and tlr5m genes cloned from the spiny eel mastacembelus armatus. | toll-like receptors (tlrs) play an important role in innate and adaptive immunity. here, we identify two new tlrs from the spiny eel mastacembelus armatus (tlr3 and membrane tlr5m). both matlr3 and matlr5m were expressed in all tested tissues; expression was highest in liver and spleen, respectively. after infection with vibrio parahaemolyticus, expression of both tlrs fluctuated and differed significantly from controls at several time points. the predicted three-dimensional model of the matlr3 ... | 2017 | 28821419 |
| christmas-tree derived amplification immuno-strategy for sensitive visual detection of vibrio parahaemolyticus based on gold label silver stain technology. | a developed christmas-tree derived immunosensor based on a gold label silver stain (glss) technique was fabricated for a highly sensitive analysis of vibrio parahaemolyticu (vp). in this strategy, captured vp antibody (cab) was immobilized on a solid substrate; then, the vps were sequentially tagged with a signal probe by incubating the assay with a detection vp antibody (dab) conjugated gold nanoparticles (aunps)-labeled graphite-like carbon nitride (g-c3n4). finally, the attached signal probe ... | 2017 | 28794324 |
| insight into the origin and evolution of the vibrio parahaemolyticus pandemic strain. | a strain of vibrio parahaemolyticus that emerged in 1995 caused the first known pandemic involving this species. this strain comprises clonal autochthonous ocean-dwelling bacteria whose evolution has occurred in the ocean environment. the low sequence diversity in this population enabled the discovery of information on its origin and evolution that has been hidden in bacterial clones that have evolved over a long period. multilocus sequencing and microarray analysis, together with phylogenetic a ... | 2017 | 28790994 |
| distribution and antimicrobial susceptibility of vibrio species associated with zooplankton in coastal area of korea. | vibrio parahaemolyticus and v. vulnificus are the most common pathogens causing seafood-borne illnesses in korea. this study determines the abundance and antimicrobial resistance of pathogenic vibrio species in seawater and zooplankton samples from the geoje island coast in korea, which is an important area for coastal fisheries, the fishing industry, and tourism. the two vibrio species were detected more in mesozooplankton samples than in seawater samples. v. parahaemolyticus isolates showed gr ... | 2017 | 28781188 |
| function of two ficolin-like proteins in innate immune defense of the oriental river prawn, macrobrachium nipponense. | ficolins have crucial functions in recognizing and eliminating pathogens in innate immunity. in this study, we identified two ficolin-like genes from the oriental river prawn, macrobrachium nipponense. these genes were designated as mnfico1 and mnfico2. mnfico1 cdna has 1600 bp, whereas mnfico2 has 1486 bp. in addition to a coiled-coil region or a low complexity region, the two ficolins both contained a signal peptide and a fibrinogen-related domain. qrt-pcr results showed that the highest expre ... | 2017 | 28764985 |
| nmr-based metabolomics reveals the metabolite profiles of vibrio parahaemolyticus under ferric iron stimulation. | vibrio parahaemolyticus is a halophilic bacterium endemic to coastal areas, and its pathogenicity has caused widespread seafood poisoning. in our previous research, the protein expression of v. parahaemolyticus in fe(3+) medium was determined using isobaric tags for relative and absolute quantitation (itraq). here, nuclear magnetic resonance (nmr) was used to detect changes in the v. parahaemolyticus metabolome. nmr spectra were obtained using methanol-water extracts of intracellular metabolites ... | 2017 | 28752295 |
| identification of an mitf gene and its polymorphisms associated with the vibrio resistance trait in the clam meretrix petechialis. | microphthalmia-associated transcription factor (mitf) regulates the transcription of its target genes by binding to their promoters. in this study, an mitf gene, mpmitf was identified in the clam meretrix petechialis. the full-length cdna of mpmitf is 3564 bp with an orf of 1365 bp. the deduced amino acid sequence consists of a conserved functional structure of bhlh-lz, which could bind with e-box. the mrna and protein expression levels of mpmitf were significantly up-regulated 6 h post-vibrio i ... | 2017 | 28734968 |
| characterizing the adherence profiles of virulent vibrio parahaemolyticus isolates. | the human pathogen vibrio parahaemolyticus is a leading cause of seafood-borne illness in the usa, and infections with v. parahaemolyticus typically result from eating raw or undercooked oysters. v. parahaemolyticus has been shown to be highly resistant to oyster depuration, suggesting that the bacterium possesses specific mechanisms or factors for colonizing oysters and persisting during depuration. in this study, we characterized eight different v. parahaemolyticus strains for differences in r ... | 2017 | 28717834 |
| antibacterial activity of food-grade chitosan against vibrio parahaemolyticus biofilms. | biofilm is a community composed of microbes and the extracellular polymeric substances. this special architecture poses a significant public health risk as it increases the fitness of bacteria in harsh conditions and renders bacterial resistance to antimicrobial agents and cleaning. in this study, we investigated the inhibition and eradication effects of chitosan on the biofilm of vibrio parahaemolyticus, an important food-borne pathogen. the crystal violet staining, [2, 3-bis (2-methoxy-4-nitro ... | 2017 | 28710011 |
| molecular characterization and expression analyses of toll like receptor-5 induced by vibrio parahaemolyticus antigens in pacific red snapper. | toll-like receptor 5 (tlr5) is a member of tlrs family responsible for the bacterial flagellin recognition in vertebrates. herein, the tlr5m gene structure of pacific red snapper (lutjanus peru) was characterized. the full-length cdna of lptlr5m comprises an open reading frame (orf) of 2715 bp, encoding a polypeptide of 904 amino acids including 9 lrrs (residues 119-562) and one lrr-ct domain (residues 593-646) at the extracellular region, and a tir domain (residues 710-904) in the cytoplasmic r ... | 2017 | 28698126 |
| parallel evolution of two clades of a major atlantic endemic vibrio parahaemolyticus pathogen lineage by independent acquisition of related pathogenicity islands. | shellfish-transmitted vibrio parahaemolyticus infections have recently increased from locations with historically low disease incidence, such as the northeast united states (us). this change coincided with a bacterial population shift towards human pathogenic variants occurring in part through the introduction of several pacific native lineages (st36, st43 and st636) to near-shore areas off the atlantic coast of the northeast us. concomitantly, st631 emerged as a major endemic pathogen. phylogen ... | 2017 | 28687650 |
| characterization of clinical vibrio parahaemolyticus strains in zhoushan, china, from 2013 to 2014. | vibrio parahaemolyticus is recognized as major cause of foodborne illness of global public health concern. this study collected 107 strains of v. parahaemolyticus during active surveillance of diarrheal diseases in hospitals in zhoushan during 2013 to 2014 and investigated their serotypes, virulence genes (tdh, trh, and orf8), antimicrobial resistance, and genotypes. the dominant serotypes of the 107 clinical strains were o3:k6, o4:k8, and o4:kut with 87.9% and 3.7% of the strains carrying the v ... | 2017 | 28678810 |
| prevalence and antimicrobial susceptibility of vibrio parahaemolyticus isolated from short mackerels (rastrelliger brachysoma) in malaysia. | numerous prevalence studies and outbreaks of vibrio parahaemolyticus infection have been extensively reported in shellfish and crustaceans. information on the quantitative detection of v. parahaemolyticus in finfish species is limited. in this study, short mackerels (rastrelliger brachysoma) obtained from different retail marketplaces were monitored with the presence of total and pathogenic strains of v. parahaemolyticus. out of 130 short mackerel samples, 116 (89.2%) were detected with the pres ... | 2017 | 28659901 |
| involvement of a tachylectin-like gene and its protein in pathogenesis of acute hepatopancreatic necrosis disease (ahpnd) in the shrimp, penaeus monodon. | a shrimp disease, the so-called acute hepatopancreatic necrosis disease (ahpnd) is caused by a specific strain of vibrio parahaemolyticus (vp) and it has resulted in significant losses to the global shrimp farming industry. in our previous study, three of tachylectin-like genes were cloned and characterized from the intestine of penaeus monodon, designated as penlectin5-1 (pl5-1), penlectin5-2 (pl5-2) and penlectin5-3 (pl5-3). these three genes all contain fibrinogen-related domain (fred). the e ... | 2017 | 28655576 |
| transcriptome analysis of the hepatopancreas in exopalaemon carinicauda infected with an ahpnd-causing strain of vibrio parahaemolyticus. | acute hepatopancreatic necrosis disease (ahpnd) caused by vibrio parahaemolyticus carrying toxin-producing plasmid, has led to severe mortalities in multiple shrimp species throughout asia. in order to understand the immunological response of shrimp to infection by ahpnd-causing strain of v. parahaemolyticus (vpahpnd), the transcriptomic profiles of the hepatopancreas from severe ahpnd-infected (bs_g), ahpnd-survived (kb_g) and non-infected (pbs_g) exopalaemon carinicauda groups were obtained us ... | 2017 | 28648882 |
| a comprehensive epidemiological research for clinical vibrio parahaemolyticus in shanghai. | vibrio parahaemolyticus is one of the most important pathogen for seafood-borne gastroenteritis in shanghai and the rest of the world. a total of 42 v. parahaemolyticus strains were isolated from 1900 fecal specimens collected from patients in shanghai hospital presenting from january 2014 to december 2015. all isolates were evaluated for potential virulence factors [tdh, trh, and type three secretion system (t3ss) genes], typed using multilocus sequence typing (mlst) and screened for antimicrob ... | 2017 | 28642752 |
| t3ss effector vopl inhibits the host ros response, promoting the intracellular survival of vibrio parahaemolyticus. | the production of antimicrobial reactive oxygen species by the nicotinamide dinucleotide phosphate (nadph) oxidase complex is an important mechanism for control of invading pathogens. herein, we show that the gastrointestinal pathogen vibrio parahaemolyticus counteracts reactive oxygen species (ros) production using the type iii secretion system 2 (t3ss2) effector vopl. in the absence of vopl, intracellular v. parahaemolyticus undergoes ros-dependent filamentation, with concurrent limited growth ... | 2017 | 28640881 |
| comparative transcriptome analysis of sinonovacula constricta in gills and hepatopancreas in response to vibrio parahaemolyticus infection. | the razor clam sinonovacula constricta is an important economic species in china. however, bacterial pathogenic diseases limits s. constricta farming industry for large-scale production. in this study, de novo transcriptome sequencing was performed on s. constricta gills and hepatopancreas under vibrio parahaemolyticus challenge for 12 h and 48 h, respectively. transcripts assembly constructed 18,330 sequences, each of which was 500 bp long and functionally annotated, and 1781 and 490 transcript ... | 2017 | 28630016 |
| survival of litopenaeus vannamei shrimp fed on diets supplemented with dunaliella sp. is improved after challenges by vibrio parahaemolyticus. | survival of litopenaeus vannamei was evaluated during a vibrio parahaemolyticus infection. this bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (ems), also known as acute hepatopancreatic necrosis disease (ahpnd). control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. new methods have been proposed to control and prevent the impact of bacterial ... | 2017 | 28625840 |
| pathogenicity profile of vibrio parahaemolyticus in farmed pacific white shrimp, penaeus vannamei. | a pathobiological study was conducted using vibrio parahaemolyticus (vp) strain isolated from vibriosis affected shrimp (penaeus vannamei) farms in kancheepuram and thiruvallur districts of tamil nadu during august 2014 to february 2015. the isolate was identified based on the morphological, physiological, biochemical and molecular characters. ld50 value with intramuscular injection was determined as 2.6 × 10(4) cfu/shrimp and sequential pathology was studied giving 6.1 × 10(3) cfu/shrimp (ld25) ... | 2017 | 28606862 |
| genomic and transcriptomic analyses reveal distinct biological functions for cold shock proteins (vpacspa and vpacspd) in vibrio parahaemolyticus chn25 during low-temperature survival. | vibrio parahaemolyticus causes serious seafood-borne gastroenteritis and death in humans. raw seafood is often subjected to post-harvest processing and low-temperature storage. to date, very little information is available regarding the biological functions of cold shock proteins (csps) in the low-temperature survival of the bacterium. in this study, we determined the complete genome sequence of v. parahaemolyticus chn25 (serotype: o5:kut). the two main csp-encoding genes (vpacspa and vpacspd) w ... | 2017 | 28583064 |
| a natural vibrio parahaemolyticus pir(vp)a(-)b(+) mutant kills shrimp but produces no pir(vp) toxins or ahpnd lesions. | acute hepatopancreatic necrosis disease (ahpnd) of shrimp is caused by vibrio parahaemolyticus (vp) isolates that harbor a pva plasmid encoding toxins pir(vp)a and pir(vp)b (vpahpnd). these are released from vpahpnd that colonize the shrimp stomach and produce pathognomonic ahpnd lesions (massive sloughing of hepatopancreatic tubule epithelial cells). pcr results indicated that vp isolate xn87 lacked pir(vp)a but carried pir(vp)b unexpectedly, western blot analysis of proteins from culture broth ... | 2017 | 28576761 |
| spreading of pandemic vibrio parahaemolyticus o3:k6 and its serovariants: a re-analysis of strains isolated from multiple studies. | in china, v. parahaemolyticus has been a leading cause of foodborne outbreaks and bacterial infectious diarrhea since the 1990s, and most infections have been associated with the pandemic v. parahaemolyticus o3:k6 and its serovariants. however, a comprehensive overview of the sero-prevalence and genetic diversity of the pandemic v. parahaemolyticus clone in china is lacking. to compensate for this deficiency, pandemic isolates in both clinical and environmental chinese samples collected from mul ... | 2017 | 28573108 |
| induction of cellular differentiation and single cell imaging of vibrio parahaemolyticus swimmer and swarmer cells. | the ability to study the intracellular localization of proteins is essential for the understanding of many cellular processes. in turn, this requires the ability to obtain single cells for fluorescence microscopy, which can be particularly challenging when imaging cells that exist within bacterial communities. for example, the human pathogen vibrio parahaemolyticus exists as short rod-shaped swimmer cells in liquid conditions that upon surface contact differentiate into a subpopulation of highly ... | 2017 | 28570527 |
| different abundance and correlational patterns exist between total and presumed pathogenic vibrio vulnificus and v. parahaemolyticus in shellfish and waters along the north carolina coast. | monitoring of vibrio vulnificus and v. parahaemolyticus abundance is pertinent due to the ability of these species to cause disease in humans through aquatic vectors. previously, we performed a multiyear investigation tracking vibrio spp. levels in five sites along the southeastern north carolina coast. from february 2013 to october 2015, total v. vulnificus and v. parahaemolyticus abundance was measured in water, oysters and clams. in the current study, pathogenic subpopulations were identified ... | 2017 | 28531284 |
| gene expression of vibrio parahaemolyticus growing in laboratory isolation conditions compared to those common in its natural ocean environment. | vibrio parahaemolyticus is an autochthonous marine bacterial species comprising strains able to grow in broth containing bile salts at 37 °c, a condition seldom found in the ocean. however, this condition is used for isolation in the laboratory because it is considered a necessary property for pathogenesis. in this context, revealing how gene expression enables v. parahaemolyticus to adapt to this particular condition -common to almost all v. parahaemolyticus isolates- will improve our understan ... | 2017 | 28525966 |
| molecular typing of vibrio parahaemolyticus strains isolated from mollusks in the north adriatic sea. | vibrio parahaemolyticus is an emerging foodborne pathogen in the mediterranean, usually associated with shellfish consumption. the increase in the number of outbreaks in europe is primarily associated with the global warming of the ocean that has a great impact on the spread and genetic selection of waterborne pathogens. the primary role of italy in europe's mollusk production, together with the fact that cases of infections with v. parahaemolyticus are not always notified to the european commun ... | 2017 | 28514180 |
| science signaling podcast for 16 may 2017: vibrio rewires host cells. | this podcast features a conversation with kim orth and nicole de nisco, authors of a research resource that appears in the 16 may 2017 issue of science signaling, about how the marine bacterium vibrio parahaemolyticus rewires host cell signaling networks. v. parahaemolyticus thrives in warm brackish waters and infects both shellfish and finfish. this bacterium causes gastroenteritis when humans consume contaminated seafood that is raw or undercooked. v. parahaemolyticus delivers virulence factor ... | 2017 | 28512146 |
| the cytotoxic type 3 secretion system 1 of vibrio rewires host gene expression to subvert cell death and activate cell survival pathways. | bacterial effectors potently manipulate host signaling pathways. the marine bacterium vibrio parahaemolyticus (v. para) delivers effectors into host cells through two type 3 secretion systems (t3sss). t3ss1 is vital for v. para survival in the environment, whereas t3ss2 causes acute gastroenteritis in human hosts. although the natural host is undefined, t3ss1 effectors attack highly conserved cellular processes and pathways to orchestrate nonapoptotic cell death. to understand how the concerted ... | 2017 | 28512145 |
| new sequence types of vibrio parahaemolyticus isolated from a malaysian aquaculture pond, as revealed by whole-genome sequencing. | the acquisition of photorhabdus insect-related (pir) toxin-like genes in vibrio parahaemolyticus has been linked to hepatopancreatic necrosis disease in shrimp. we report the whole-genome sequences of genetically virulent and avirulent v. parahaemolyticus isolated from a malaysian aquaculture pond and show that they represent previously unreported sequence types of v. parahaemolyticus. | 2017 | 28495773 |