| prevention of tnbs-induced colitis by different lactobacillus and bifidobacterium strains is associated with an expansion of gammadeltat and regulatory t cells of intestinal intraepithelial lymphocytes. | probiotics may protect against inflammatory bowel disease through regulation of lamina propria lymphocytes (lpls) function. data are lacking on possible involvement of intraepithelial lymphocytes (iels). the aim of this study was to investigate whether different probiotic mixtures prevented gut inflammatory disease and the role of both iels and lpls. | 2009 | 19504616 |
| genetically modified bifidobacterium displaying salmonella-antigen protects mice from lethal challenge of salmonella typhimurium in a murine typhoid fever model. | we developed a novel vaccine platform utilizing bifidobacterium as an antigen delivery vehicle for mucosal immunization. genetically modified bifidobacterium longum displaying salmonella-flagellin on the cell surface was constructed for the oral typhoid vaccine. the efficiency of this vaccine was evaluated in a murine model of typhoid fever. we then orally administered 2.5 × 10(7) cfu of the recombinant bifidobacterium longum (vaccine) or parental bifidobacterium longum, or pbs to balb/c mice ev ... | 2010 | 20709009 |
| in vitro effects of phosphatidylcholine and transgalactooligosaccharides on the production of 1,2-sn-diacylglycerol by bifidobacterium longum biovar infantis. | to investigate the production of the tumour promoter 1,2-sn-diacylglycerol (dag) by a human gut isolate of bifidobacterium longum biovar infantis. | 2008 | 18828790 |
| probiotics in the treatment of acute rotavirus diarrhoea. a randomized, double-blind, controlled trial using two different probiotic preparations in bolivian children. | evidence suggests that probiotics reduce rotavirus diarrhoea duration. although there are several probiotic strains potentially useful, daily practice is often limited by the type and number of products locally available. in general, information about combined products is scarce. in this study we compare the effect of two probiotic products in the treatment of diarrhoea in children less than 2 years of age. | 2010 | 20735858 |
| broad conservation of milk utilization genes in bifidobacterium longum subsp. infantis as revealed by comparative genomic hybridization. | human milk oligosaccharides (hmos) are the third-largest solid component of milk. their structural complexity renders them nondigestible to the host but liable to hydrolytic enzymes of the infant colonic microbiota. bifidobacteria and, frequently, bifidobacterium longum strains predominate the colonic microbiota of exclusively breast-fed infants. among the three recognized subspecies of b. longum, b. longum subsp. infantis achieves high levels of cell growth on hmos and is associated with early ... | 2010 | 20802066 |
| resistance to acidic environments of caries-associated bacteria: bifidobacterium dentium and bifidobacterium longum. | oral bifidobacteriaceae, bifidobacterium dentium and bifidobacterium longum, are known to be isolated together with mutans streptococci and lactobacilli from caries lesions, suggesting that these bifidobacteriaceae are caries associated and acid resistant. this study aimed to investigate effects of acidification on b. dentium and b. longum, and to compare them with those on streptococcus mutans, streptococcus sanguinis and lactobacillus paracasei. effects of acidification, growth ability in a co ... | 2010 | 20814202 |
| ribosomal protein profiling by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for phylogenety-based subspecies resolution of bifidobacterium longum. | the taxonomic positions of the subspecies of bifidobacterium longum (b. longum subsp. longum, subsp. infantis, and subsp. suis) have been controversial. a current proposal is that the former two species "b. infantis" and "b. suis" be unified with b. longum and all three reclassified as three subspecies. to test this proposal, ribosomal protein profiling as observed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) was applied to the classification of ... | 2011 | 20828962 |
| immunomodulatory properties of fermented soy and dairy milks prepared with lactic acid bacteria. | fermented soy and dairy milk preparations provide a means for delivering lactic acid bacteria and their fermentation products into the diet. our aims were to test immunomodulatory bioactivity of fermented soy beverage (sb) and dairy milk blend (mb) preparations on human intestinal epithelial cells (iec) and to determine the impact of freezing medium on culture survival prior to bioactivity analyses. fermented sb and mb were prepared using pure or mixed cultures of streptococcus thermophilus st5, ... | 2009 | 19799669 |
| lactic fermentation and antioxidant activity of zingiberaceae plants in taiwan. | the present study evaluated functional properties of lactic-fermented ginger products. three zingiberaceae species were used as the substrate for fermentation using three lactic acid bacteria. the fermentation process ended 35-40 h after inoculation and reached a ph value of 3.5-4.0. total antioxidant performances were 68-75%, and were best observed using bifidobacterium longum as the starter in three ginger samples. dpph scavenging was on average 70%, with free radical anion scavenging and pero ... | 2009 | 18946800 |
| exopolysaccharides produced by lactobacillus and bifidobacterium strains abrogate in vitro the cytotoxic effect of bacterial toxins on eukaryotic cells. | to evaluate the capability of the exopolysaccharides (eps) produced by lactobacilli and bifidobacteria from human and dairy origin to antagonize the cytotoxic effect of bacterial toxins. | 2010 | 20846331 |
| polymorphism and distribution of putative cell-surface adhesin-encoding orfs among human fecal isolates of bifidobacterium longum subsp. longum. | the polymorphism of orfs encoding putative cell-surface adhesins was investigated in bifidobacterium longum subsp. longum. firstly, we performed a pcr assay targeting 15 orfs encoding putative adhesion proteins, which included 8 orfs with a sortase targeting lpxtg motif, in 42 strains of different pulsotypes isolated from fecal samples from 12 human individuals. we found a variability in the presence of an orf, bl0675, which encodes a putative fimbrial subunit protein. we sequenced orfs correspo ... | 2011 | 20862609 |
| effects of probiotic bacteria and their genomic dna on th1/th2-cytokine production by peripheral blood mononuclear cells (pbmcs) of healthy and allergic subjects. | among the factors potentially involved in the increased prevalence of allergic diseases, modification of the intestinal flora or lack of microbial exposure during childhood has been proposed. t(h)2-cytokines increase the production of ige and stimulate mast cells and eosinophils, whereas t(h)1-cytokines, such as ifn-gamma, may suppress ige synthesis and stimulate the expression of the secretory piece of iga. thus, a dysregulation in the expression of t(h)1- and t(h)2-cytokines may contribute to ... | 2008 | 18950596 |
| exopolysaccharide activities from probiotic bifidobacterium: immunomodulatory effects (on j774a.1 macrophages) and antimicrobial properties. | exopolysaccharides (epss) are a metabolite of probiotics which have gained wide interest recently, but little is known about their function. eps was isolated from bifidobacterium longum bcrc 14634 and sterilized by 0.22 μm filter. the proliferation of j77a.1 macrophages and their secretion of the anti-inflammatory cytokine interleukin-10 (il-10) was elevated after treatment with heat-killed b. longum or 5 μg/ml eps. the endotoxin, lipopolysaccharides (lps), a potent inducer of pro-inflammatory c ... | 2010 | 20884069 |
| comparative analysis of the properties of bifidobacterial isolates from fecal samples of mother-infant pairs. | the aim of the study was to evaluate the possibility of bifidobacterium breve transfer from the mother to her infant during the perinatal period. | 2010 | 20890213 |
| immunomodulatory impact of a synbiotic in t(h)1 and t(h)2 models of infection. | the immunomodulatory activity of a synbiotic combination containing three bacterial strains (lactobacillus helveticus r0052, bifidobacterium longum subsp. infantis r0033 and bifidobacterium bifidum r0071) and short-chain fructooligosaccharide was examined in two distinct infectious rat models. in the t(h)1 model, wistar rats were administered the synbiotic combination for 2 weeks prior to challenge with a single oral dose of enterotoxigenic escherichia coli or vehicle. in the t(h)2 model, pretre ... | 2010 | 20929951 |
| bifidobacterium strains suppress in vitro the pro-inflammatory milieu triggered by the large intestinal microbiota of coeliac patients. | abstract: | 2008 | 18980693 |
| effect of production conditions on the stability of a human bifidobacterial species bifidobacterium longum in yogurt. | human bifidobacteria are more sensitive to external environmental factors than animal bifidobacteria, and it is difficult to ensure their stable survival in yogurt. the purpose of this investigation was to observe the survival of human bifidobacteria in yogurts produced under various production conditions. | 2009 | 19818009 |
| assessment of psychotropic-like properties of a probiotic formulation (lactobacillus helveticus r0052 and bifidobacterium longum r0175) in rats and human subjects. | in a previous clinical study, a probiotic formulation (pf) consisting of lactobacillus helveticus r0052 and bifidobacterium longum r0175 (pf) decreased stress-induced gastrointestinal discomfort. emerging evidence of a role for gut microbiota on central nervous system functions therefore suggests that oral intake of probiotics may have beneficial consequences on mood and psychological distress. the aim of the present study was to investigate the anxiolytic-like activity of pf in rats, and its po ... | 2010 | 20974015 |
| probiotic food supplement reduces stress-induced gastrointestinal symptoms in volunteers: a double-blind, placebo-controlled, randomized trial. | stress plays an important role in the development of symptoms contributing to disease. stress induces various disorders with gastrointestinal, physical, and psychological symptoms. probiotics can help regulate or modulate gastrointestinal functions. the aim of the present study was to investigate the effects of a probiotic preparation (probio-stick) on stress-induced symptoms in volunteers. a double-blind, placebo-controlled, randomized study was conducted on volunteers with symptoms of stress. ... | 2008 | 19083380 |
| in vitro th1 cytokine-independent th2 suppressive effects of bifidobacteria. | a comparison between 17 strains of lactic acid bacteria and 15 strains of bifidobacteria indicated that bifidobacteria induced significantly lower levels of interleukin-12 (il-12) in murine splenic cells. the present study aims to evaluate the effect and mechanism of bifidobacterium longum bb536, a probiotic strain, in suppressing antigen-induced th2 immune response in vitro. bb536 suppressed immunoglobulin (ig) e and il-4 production by ovalbumin-sensitized splenic cells, but induction of th1-in ... | 2007 | 17641467 |
| effect of fermented milk containing lactobacillus acidophilus and bifidobacterium longum on plasma lipids of women with normal or moderately elevated cholesterol. | this study aimed to evaluate the effect of milk fermented with lactobacillus acidophilus 145 and bifidobacterium longum bb536 on plasma lipids in a sample of adult women. a double-blind, placebo controlled, cross-over study (two periods of four weeks each separated by a 1-week washout period) was performed in 34 women, aged between 18 and 65 years. group a consumed 125 g fermented milk three times a day for the first 4 weeks while group b consumed regular yoghurt under the same conditions. (grou ... | 2009 | 19825213 |
| complete genome sequence of bifidobacterium longum subsp. longum bbmn68, a new strain from a healthy chinese centenarian. | bifidobacterium longum subsp. longum bbmn68 was isolated from the feces of a healthy centenarian living in an area of bama, guangxi, china, known for longevity. here we report the main genome features of b. longum strain bbmn68 and the identification of several predicted proteins associated with the ecological niche of longevity. | 2010 | 21097614 |
| bifidobacterium longum as a delivery system of trail and endostatin cooperates with chemotherapeutic drugs to inhibit hypoxic tumor growth. | in our previous study, we have shown that vector pbv22210 containing a chloramphenicol resistance and a cryptic plasmid pmb1 from bifidobacterium longum strain could stably replicate and did not significantly affect the biological characteristics of b. longum. in this study, b. longum was transfected by electroporation with pbv22210 encoding the extracellular domain of trail (b. longum-pbv22210-trail) and its carbohydrate fermentation and growth curve were determined, and its location and inhibi ... | 2009 | 19229287 |
| β-lactoglobulin tablets as a suitable vehicle for protection and intestinal delivery of probiotic bacteria. | the use of succinylated β-lactoglobulin as a novel functional tablet excipient for the protection of probiotic bacteria against the adverse gastric conditions and their delivery in the intestine was studied. tablets were produced by direct compression of a dry mixture of bifidobacterium longum ha-135 and the tested excipient. the results showed that tablets made of native β-lg did not ensure cell survival while grafting carboxylic acid groups on the protein revealed to be an innovative method to ... | 2010 | 21129464 |
| high-throughput method for comparative analysis of denaturing gradient gel electrophoresis profiles from human fecal samples reveals significant increases in two bifidobacterial species after inulin-type prebiotic intake. | denaturing gradient gel electrophoresis (dgge) is one of the most commonly used molecular tools to study complex microbial communities. despite its widespread use, meaningful interpretative analysis remains a major drawback of this method. we evaluated the combination of computer-assisted band-matching with nonparametric statistics for comparative analysis of dgge banding patterns. fecal samples from 17 healthy volunteers who consumed 20 g of the prebiotic compound oligofructose-enriched inulin ... | 2010 | 21133959 |
| molecular cloning and characterization of a beta-l-arabinobiosidase in bifidobacterium longum that belongs to a novel glycoside hydrolase family. | extensin is a glycoprotein that is rich in hydroxyprolines linked to β-l-arabinofuranosides. in this study, we cloned a hypba2 gene that encodes a novel β-l-arabinobiosidase from bifidobacterium longum jcm 1217. this enzyme does not have any sequence similarity with other glycoside hydrolase families but has 38-98% identity to hypothetical proteins in bifidobacterium and xanthomonas strains. the recombinant enzyme liberated l-arabinofuranose (araf)-β1,2-araf disaccharide from carrot extensin, po ... | 2010 | 21149454 |
| effects of multistrain probiotic-containing yogurt on second-line triple therapy for helicobacter pylori infection. | the adjuvant effects of probiotic-containing yogurt on second-line triple therapy for helicobacter pylori (h. pylori) infection have not been evaluated. | 2011 | 21175792 |
| clostridium difficile colonization in early infancy is accompanied by changes in intestinal microbiota composition. | clostridium difficile is a major enteric pathogen responsible for antibiotic-associated diarrhea. host susceptibility to c. difficile infections results partly from inability of the intestinal microbiota to resist c. difficile colonization. during early infancy, asymptomatic colonization by c. difficile is common and the intestinal microbiota shows low complexity. thus, we investigated the potential relationship between the microbiota composition and the implantation of c. difficile in infant gu ... | 2010 | 21177896 |
| in vitro fermentation of rice bran combined with lactobacillus acidophilus 14 150b or bifidobacterium longum 05 by the canine faecal microbiota. | the fermentability of rice bran (rb), alone or in combination with one of two probiotics, by canine faecal microbiota was evaluated in stirred, ph-controlled, anaerobic batch cultures. rb enhanced the levels of bacteria detected by probes bif164 (bifidobacteria) and lab158 (lactic acid bacteria); however, addition of the probiotics did not have a significant effect on the predominant microbial counts compared with rb alone. rb sustained levels of bifidobacterium longum 05 throughout the fermenta ... | 2011 | 21204868 |
| lactobacillus helveticus and bifidobacterium longum taken in combination reduce the apoptosis propensity in the limbic system after myocardial infarction in a rat model. | myocardial infarction (mi) stimulates the release of pro-inflammatory substances that induce apoptosis in the limbic system. pro-inflammatory cytokines are considered as the root cause of apoptosis, although the mechanism is not fully explained and/or understood at this time. in addition, depression may induce gastrointestinal perturbations that maintain the elevated levels of pro-inflammatory cytokines. it has been shown that some specific probiotic formulations may reduce gastrointestinal prob ... | 2009 | 19563693 |
| distinctive anti-allergy properties of two probiotic bacterial strains in a mouse model of allergic poly-sensitization. | we compared the immunomodulatory properties of bifidobacterium longum ncc 3001 and lactobacillus paracasei ncc 2461 in a mouse model of poly-sensitization to birch and grass pollen allergens. mucosal application of both strains at the time of sensitization and challenge led to significant suppression of airway inflammation and down-regulated allergen-specific immune responses. in contrast, in the mice treated with probiotics prior to sensitization and challenge, only b. longum displayed protecti ... | 2011 | 21216308 |
| faecal bifidobacteria in indian neonates & the effect of asymptomatic rotavirus infection during the first month of life. | bifidobacteria colonize the gut after the first week of life and remain an important component of the gut microbiota in infancy. this study was carried out to characterize the diversity and number of bifidobacteria colonizing the gut in indian neonates and to investigate whether asymptomatic infection with rotavirus in the first month of life affected gut colonization by bifidobacteria. | 2010 | 21245621 |
| improved tolerance to bile salts of aggregated bifidobacterium longum produced during continuous culture with immobilized cells. | the effect of cell immobilization and continuous culture was studied on selected physiological and technological characteristics of bifidobacterium longum ncc2705 cultivated for 20 days in a two stage continuous fermentation system. continuous immobilized cell (ic) cultures with and without glucose limitation exhibited formation of macroscopic cell aggregates after 12 and 9 days, respectively. auto-aggregation resulted in underestimation of viable cell counts by plate counts by more than 2 log u ... | 2010 | 21257307 |
| a versatile and scalable strategy for glycoprofiling bifidobacterial consumption of human milk oligosaccharides. | human milk contains approximately 200 complex oligosaccharides believed to stimulate the growth and establishment of a protective microbiota in the infant gut. the lack of scalable analytical techniques has hindered the measurement of bacterial metabolism of these and other complex prebiotic oligosaccharides. an in vitro, multi-strain, assay capable of measuring kinetics of bacterial growth and detailed oligosaccharide consumption analysis by fticr-ms was developed and tested simultaneously on 1 ... | 2009 | 21261928 |
| in vitro fermentation of arabinoxylan-derived carbohydrates by bifidobacteria and mixed fecal microbiota. | bifidobacterium adolescentis atcc 15703, bifidobacterium breve atcc 15700, bifidobacterium longum atcc 15707, and human fecal microbiota were cultivated in vitro with d-xylose, l-arabinose, xylo-oligosaccharides (xos), and arabinoxylo-oligosaccharides (axos) as carbon sources. the ph, formation of volatile fatty acids, and carbohydrate utilization profiles were followed. in the pure bifidobacteria cultures optical density and in the fecal slurries pressure and h(2) were also detected. a differin ... | 2009 | 19694435 |
| an infant-associated bacterial commensal utilizes breast milk sialyloligosaccharides. | lactating mothers secrete milk sialyloligosaccharides (msos) that function as anti-adhesives once provided to the neonate. particular infant-associated commensals, such as bifidobacterium longum subsp. infantis, consume neutral milk oligosaccharides, although their ability to utilize acidic oligosaccharides has not been assessed. temporal glycoprofiling of acidic hmo consumed during fermentation demonstrated a single composition, with several isomers, corresponding to sialylated lacto-n-tetraose ... | 2011 | 21288901 |
| oral administration of live bifidobacterium substrains isolated from centenarians enhances intestinal function in mice. | we studied the effects of two bifidobacteria strains isolated from healthy centenarians on intestinal function in mice. bifidobacterium adolescentis bbmn23 and bifidobacterium longum bbmn68 were orally administrated to specific pathogen-free balb/c mice at different doses (2 x 10(11), 2 x 10(9), or 2 x 10(7) cfu/kg body weight) each day for 4 weeks. villus height, crypt depth, villus width, and villus/crypt ratio (v/c) were determined. the content of duodenal secreted immunoglobulin a (siga) was ... | 2009 | 19701668 |
| sugar source modulates exopolysaccharide biosynthesis in bifidobacterium longum subsp. longum crc 002. | the effect of four sugars (glucose, galactose, lactose and fructose) on exopolysaccharide (eps) production by bifidobacterium longum subsp. longum crc 002 was evaluated. more eps was produced when crc 002 was grown on lactose in the absence of ph control, with a production of 1080+/-120 mg eps l(-1) (p<0.01) after 24 h of incubation. for fructose, galactose and glucose, eps production was similar, at 512+/-63, 564+/-165 and 616+/-93 mg eps l(-1), respectively. the proposed repeating unit composi ... | 2010 | 19850611 |
| bifidobacterial utilization of human milk oligosaccharides. | a promising strategy to improve health is the rational manipulation of one's beneficial microbiota via dietary interventions. this is observed in nature where specific bifidobacteria utilize human milk oligosaccharides (hmos) that are encountered within the breast-fed infant colon. bifidobacterium longum subsp. infantis is regarded as the archetypical hmo consumer associated with the developing neonate. this review summarizes the known molecular mechanisms underlying hmo utilization, as determin ... | 2011 | 21342711 |
| oxidative stress-related responses of bifidobacterium longum subsp. longum bbmn68 at the proteomic level after exposure to oxygen. | bifidobacterium longum subsp. longum bbmn68, an anaerobic probiotic isolated from healthy centenarian faeces, shows low oxygen (3?%, v/v) tolerance. to understand the effects of oxidative stress and the mechanisms protecting against it in this strain, a proteomic approach was taken to analyse changes in the cellular protein profiles of bbmn68 under the following oxygen-stress conditions. mid-exponential phase bbmn68 cells grown in mrs broth at 37 °c were exposed to 3?% o(2) for 1 h (i) or 9 h (i ... | 2011 | 21349974 |
| adherence and cytokine induction in caco-2 cells by bacterial populations from a three-stage continuous-culture model of the large intestine. | adherence of bacteria to epithelial cells is an important step in colonization and immune modulation in the large bowel. the aims of this study were to use a three-stage continuous-culture system (ccs) to investigate how environmental factors affect bacterial attachment to caco-2 cells and modulation of cytokine expression by gut microorganisms, including a probiotic bifidobacterium longum strain, dd2004. the ccs simulated environmental conditions in the proximal large intestine (vessel 1 [v1]) ... | 2011 | 21378047 |
| distribution of in vitro fermentation ability of lacto-n-biose i, a major building block of human milk oligosaccharides, in bifidobacterial strains. | this study investigated the potential utilization of lacto-n-biose i (lnb) by individual strains of bifidobacteria. lnb is a building block for the human milk oligosaccharides, which have been suggested to be a factor for selective growth of bifidobacteria. a total of 208 strains comprising 10 species and 4 subspecies were analyzed for the presence of the galacto-n-biose/lacto-n-biose i phosphorylase (glnbp) gene (lnpa) and examined for growth when lnb was used as the sole carbohydrate source. w ... | 2010 | 19854932 |
| species delineation and clonal diversity in four bifidobacterium species as revealed by multilocus sequencing. | the genus bifidobacterium comprises several species that are important contributors to the gut microbiome, with some strains having beneficial health effects. understanding the evolutionary emergence of advantageous biological properties requires knowledge of the genetic diversity and clonal structure of species. we sequenced seven housekeeping genes in 119 bifidobacterium strains of bifidobacterium animalis, bifidobacterium bifidum, bifidobacterium breve and bifidobacterium longum. phylogenetic ... | 2010 | 20060895 |
| development of a real-time rt-pcr method for enumeration of viable bifidobacterium longum cells in different morphologies. | viability of probiotic bacteria is traditionally assessed by plate counting which has several limitations, including underestimation of cells in aggregates or chains morphology. we describe a quantitative pcr (qpcr)-based method for an accurate enumeration of viable cells of bifidobacterium longum ncc2705 exhibiting different morphologies by measuring the mrna levels of cysb and purb, two constitutively expressed housekeeping genes. three primer-sets targeting short fragments of 57-bp of cyss an ... | 2010 | 20141941 |
| effects of bifidobacterium longum bb536 administration on influenza infection, influenza vaccine antibody titer, and cell-mediated immunity in the elderly. | twenty-seven elderly subjects (mean age 86.7+/-6.6 years) were pre-administered a test food containing 1x10(11) cfu of bb536 daily for 5 weeks (p1), during which they also received influenza vaccination at week 3. the subjects were then randomized to a bb536 group and a placebo group for 14 weeks (p2). the proportion of subjects who contracted influenza was significantly lower in bb536 group than in the to placebo group. the proportion of subjects with fever was also significantly lower in the b ... | 2010 | 20460726 |
| antimicrobial activity of bifidobacterium spp. isolated from healthy adult koreans against cariogenic microflora. | objective: dental caries is the main common infectious disease in the human oral cavity. streptococcus mutans and streptococcus sobrinus were reported to be the most important etiological factors in human dental caries. thus, we examined the inhibitory effects of bifidobacterium spp. cells and culture supernatants against s. mutans and s. sobrinus, including streptococcus gordonii, and aggregatibacter actinomycetemcomitans, which is associated with periodontal disease. methods: mutans streptococ ... | 2011 | 21439550 |
| the combination of mixed lactic acid bacteria and dietary fiber lowers serum cholesterol levels and fecal harmful enzyme activities in rats. | probiotics such as lactic acid bacteria (lab) and prebiotics such as fiber are generally considered beneficial for health. these affect the microflora composition and fermentation metabolites and consequently contribute to local and systemic effects in humans. the beneficial effects of probiotics can be improved when combined with prebiotics. here we investigated the effects of a mixed lab supplement combined with dietary fiber on the population of lab in the gut, as well as on serum cholesterol ... | 2011 | 21468911 |
| effects of probiotic fermentation on the enhancement of biological and pharmacological activities of codonopsis lanceolata extracted by high pressure treatment. | this study was designed to evaluate the enhancement of antioxidant, antimicrobial, enzymatic, cytotoxic, and cognitive activities of codonopsis lanceolata extracted by high pressure treatment followed by probiotic fermentation. dried c. lanceolata samples were subjected to 400mpa for 20min and then fermented with bifidobacterium longum b6 (hpe-blf) and lactobacillus rhamnosus (hpe-lrf) at 37°c for 7days. compared to conventional extraction (ce-nf, 6.69mg gae/g), the phenol amounts of hpe-blf and ... | 2011 | 21543255 |
| non-oral bifidobacteria and the aciduric microbiota of the denture plaque biofilm. | the microbiota of the denture plaque biofilm colonizing the fitting surface of dentures in edentulous subjects with healthy palates (n = 20) and in edentulous subjects with denture stomatitis (n = 20) was studied. the numbers of bacteria colonizing the dentures of healthy subjects was significantly less than the numbers colonizing the dentures of stomatitis subjects. the proportions and frequency of isolation of mutans streptococci, lactobacilli, bifidobacteria and yeasts were significantly (p < ... | 2010 | 20536746 |
| fermentation of β-glucans derived from different sources by bifidobacteria: evaluation of their bifidogenic effect. | β-glucans obtained from barley, seaweed, bacteria, and mushroom sclerotia were incubated with pure cultures of bifidobacterium infantis , bifidobacterium longum , and bifidobacterium adolescentis for a 24 h batch fermentation to evaluate their bifidogenic effect with inulin as the positive control. the ph value in all culture media was decreased by 0.5-1.5 units. all β-glucans supported the growth of the three bifidobacteria with b. infantis, having a relatively larger increase in populations (3 ... | 2011 | 21568326 |
| effect of formula composition on the development of infant gut microbiota. | : breast-feeding induces a gut microbiota rich in bifidobacteria, whereas formula-fed babies have a more diverse colonization. this ecosystem contributes to the development of the immune response and the lower incidence of diarrhea and allergy in breast-fed infants. this randomized double-blind controlled trial aimed to evaluate the bifidogenic effect of a mainly whey protein study formula low in phosphate and protein, allowing a composition closer to that of human milk. | 2011 | 21593648 |
| screening of species-specific lactic acid bacteria for veal calves multi-strain probiotic adjuncts. | the selection of promising specific species of lactic acid bacteria with potential probiotic characteristics is of particular interest in producing multi species-specific probiotic adjuncts in veal calves rearing. the aim of the present work was to select and evaluate in vitro the functional activity of lactic acid bacteria, bifidobacterium longum and bacillus coagulans strains isolated from veal calves in order to assess their potential use as multi species-specific probiotics for veal calves. ... | 2011 | 21619939 |
| targeting solid tumors with non-pathogenic obligate anaerobic bacteria. | molecular-targeting drugs with fewer severe adverse effects are attracting great attention as the next wave of cancer treatment. there exist, however, populations of cancer cells resistant to these drugs that stem from the instability of tumor cells and/or the existence of cancer stem cells, and thus specific toxicity is required to destroy them. if such selectivity is not available, these targets may be sought out not by the cancer cell types themselves, but rather in their adjacent cancer micr ... | 2010 | 20579076 |
| human intestinal microbiota: characterization of a simplified and stable gnotobiotic rat model. | the study of host microbe interactions is hampered by the complexity and inter-individual variability of the human gut microbiota. therefore, a simplified human intestinal microbiota (sihumi) consisting of seven bacterial species was introduced into germfree rats. species selection was based on numerical importance and fermentative abilities in the human gut. association of the rats with the sihumi (anaerostipes caccae, bacteroides thetaiotaomicron, bifidobacterium longum, blautia producta, clos ... | 2010 | 21637015 |
| influence of bifidobacterium longum cect 7347 and gliadin peptides on intestinal epithelial cell proteome. | celiac disease is an enteropathy caused by an abnormal immune response to cereal gluten proteins (gliadin). to unravel the possible role of the interactions between gliadin peptides and specific intestinal bacteria, the response of intestinal epithelial (caco-2) cells to gliadin subjected to gastrointestinal digestion in the presence or absence of bifidobacterium longum cect 7347 has been studied. changes in the proteome of caco-2 cells were determined by 2de and maldi-tof. gliadins digested wit ... | 2011 | 21651295 |
| temporal development of the infant gut microbiota in immunoglobulin e-sensitized and nonsensitized children determined by the ga-map infant array. | at birth, the human infant gut is sterile, but it becomes fully colonized within a few days. this initial colonization process has a major impact on immune development. our knowledge about the correlations between aberrant colonization patterns and immunological diseases, however, is limited. the aim of the present work was to develop the ga-map (genetic analysis microbiota array platform) infant array and to use this array to compare the temporal development of the gut microbiota in ige-sensiti ... | 2011 | 21653746 |
| xylitol does not inhibit xylose fermentation by engineered saccharomyces cerevisiae expressing xyla as severely as it inhibits xylose isomerase reaction in vitro. | efficient fermentation of xylose, which is abundant in hydrolysates of lignocellulosic biomass, is essential for producing cellulosic biofuels economically. while heterologous expression of xylose isomerase in saccharomyces cerevisiae has been proposed as a strategy to engineer this yeast for xylose fermentation, only a few xylose isomerase genes from fungi and bacteria have been functionally expressed in s. cerevisiae. we cloned two bacterial xylose isomerase genes from anaerobic bacteria (bact ... | 2011 | 21655987 |
| changes of fecal bifidobacterium species in adult patients with hepatitis b virus-induced chronic liver disease. | the beneficial effects of bifidobacteria on health have been widely accepted. patients with chronic liver disease have varying degrees of intestinal microflora imbalance with a decrease of total bifidobacterial counts. since different properties have been attributed to different bifidobacterium species and there is no information available for the detailed changes in the genus bifidobacterium in patients with chronic liver disease heretofore, it is meaningful to investigate the structure of this ... | 2011 | 21814872 |
| oral administration of bifidobacterium longum ameliorates influenza virus infection in mice. | we investigated whether the oral administration of bifidobacterium longum bb536 could ameliorate influenza virus (ifv) infection in a mice model. mice were orally administrated bb536 or saline for 2 weeks and then infected with ifv. orally administered bb536 significantly alleviated symptoms, reduced the loss of body weight, and inhibited viral proliferation in the lungs relative to the control group findings. histopathological findings in the lungs were improved in the bb536 group compared to c ... | 2011 | 21804232 |
| substrate promiscuity of n-acetylhexosamine 1-kinases. | n-acetylhexosamine 1-kinase (nahk) catalyzes the direct addition of a phosphate from adenosine 5'-triphosphate (atp) to the anomeric position of n-acetylhexosamine and shows similar activity towards n-acetylglucosamine (glcnac) and n-acetylgalactosamine (galnac). herein we report the cloning, characterization, and substrate specificity studies of two nahks from bifidobacterium infantis atcc15697 and bifidobacterium longum atcc55813, respectively. a new capillary electrophoresis assay method has ... | 2011 | 21799473 |
| amoxicillin treatment modifies the composition of bifidobacterium species in infant intestinal microbiota. | amoxicillin is a beta-lactam antibiotic largely used in childhood. however only few studies described its impact on composition of children gut microbiota, in particular on bifidobacterium populations considered as beneficial microorganisms. in this study, the impact on faecal bifidobacterium species of a seven-day amoxicillin treatment was quantitatively and qualitatively assessed in infants during an episode of acute respiratory infection. | 2010 | 20601031 |
| efficiency of pcr-based methods in discriminating bifidobacterium longum ssp. longum and bifidobacterium longum ssp. infantis strains of human origin. | bifidobacterium longum is considered to play an important role in health maintenance of the human gastrointestinal tract. probiotic properties of bifidobacterial isolates are strictly strain-dependent and reliable methods for the identification and discrimination of this species at both subspecies and strain levels are thus required. differentiation between b. longum ssp. longum and b. longum ssp. infantis is difficult due to high genomic similarities. in this study, four molecular-biological me ... | 2011 | 21756944 |
| temporal variations in early gut microbial colonization are associated with allergen-specific immunoglobulin e but not atopic eczema at 2 years of age. | background intestinal microbiota undergoes substantial development during the first 2 years of life, important for intestinal immunologic development and maturation influencing systemic immune responses. objective we aimed to investigate, using a prospective study design, whether allergen-specific ige (sige) and atopic eczema are associated with variations in gut microbial colonization patterns in an unselected population during the first 2 years of life. methods faeces from 94 infants were repe ... | 2011 | 21749499 |
| dissimilarity in the occurrence of bifidobacteriaceae in vaginal and perianal microbiota in women with bacterial vaginosis. | recent data point at the similarity between the perianal and vaginal microflora in terms of lactobacillus species involved. bacterial vaginosis, the most common perturbation of the vaginal microflora involving primarily overgrowth of gardnerella vaginalis, has also been suggested to involve a recto-vaginal pathway. we addressed this issue with regard to bacteria of the bifidobacteriaceae family. in particular, we investigated the putative concordance of the presence of g. vaginalis and a series ... | 2010 | 20620215 |
| the role of components of bifidobacterium and lactobacillus in pathogenesis and serologic diagnosis of autoimmune thyroid diseases. | during recent years, researchers have been focusing on the concept of an infectious etiology of autoimmune diseases. the most discussed theory is molecular mimicry, i.e. the emergence of autoreactive clones of t- and b-lymphocytes as a result of cross-immune response to homologous bacterial or viral antigen. information on the role of probiotic microorganisms (pm) in the molecular mechanisms of autoimmune thyroid diseases (atd) is limited. using proteins and immunogenic peptides databanks and re ... | 2011 | 21831795 |
| crystal structure of bifidobacterium longum phosphoketolase; key enzyme for glucose metabolism in bifidobacterium. | the crystal structure of bifidobacterium longum phosphoketolase, a thiamine diphosphate (tpp) dependent enzyme, has been determined at 2.2a resolution. the enzyme is a dimer with the active sites located at the interface between the two identical subunits with molecular mass of 92.5 kda. the bound tpp is almost completely shielded from solvent except for the catalytically important c2-carbon of the thiazolium ring, which can be accessed by a substrate sugar through a narrow funnel-shaped channel ... | 2010 | 20674574 |
| effect of fermentation by pure and mixed cultures of streptococcus thermophilus and lactobacillus helveticus on isoflavone and b-vitamin content of a fermented soy beverage. | enhancement of nutritional or vitamin content of foods is commonly touted as a major benefit of probiotics. in this paper, we examined the ability of three probiotic bacteria either alone or in combination to enhance nutritional content. pure and mixed cultures of streptococcus thermophilus st5 and lactobacillus helveticus r0052 or bifidobacterium longum r0175 were used to prepare fermented soy beverages. the effects of strain, acidification and mixed cultures on the deconjugation of soy isoflav ... | 2010 | 20688240 |
| the viability to a wall shear stress and propagation of bifidobacterium longum in the intensive membrane bioreactor. | bifidobacterium longum grew at 65-ál pilot scale of the membrane bioreactor (mbr), externally fitted with ceramic membrane (0.7-ám(2)). cell mass at the mbr reached 22.18-ágôçël(-1) as dry cell weight in 12-áh, which is 8.44 times higher than cell mass attained at the vial culture. the growth rate in the vial culture was ++ôçë=ôçë0.385-áh(-) and at the batch culture was ++ôçë=ôçë1.13-áh(-) in the exponential period and ++ôçë=ôçë0.31-áh(-1) in the stationary period. in the fed-batch mode was ++ôç ... | 2011 | 21681403 |
| transcription analysis of a lantibiotic gene cluster from bifidobacterium longum djo10a. | bifidobacterium longum djo10a was previously demonstrated to produce a lantibiotic, but only during growth on agar media. to evaluate the feasibility of production of this lantibiotic in broth media, a transcription analysis of the lana gene was undertaken. comparative microarray analysis of broth and agar cultures of b. longum djo10a revealed that the lantibiotic production, modification, transport/peptidase, and immunity genes were significantly upregulated in agar cultures, while the two-comp ... | 2011 | 21742926 |
| in vitro binding of mutagenic heterocyclic aromatic amines by bifidobacterium pseudocatenulatum g4. | consumption of probiotics has been associated with decreased risk of colon cancer and reported to have antimutagenic/ anti-carcinogenic properties. one possible mechanism for this effect involves physical binding of the mutagenic compounds, such as heterocyclic amines (hcas), to the bacteria. therefore, the objective of this study was to examine the binding capacity of bifidobacterial strains of human origin on mutagenic heterocyclic amines which are suspected to play a role in human cancers. in ... | 2010 | 21831754 |
| Bifidobacterium longum supplementation improved high-fat-fed-induced metabolic syndrome and promoted intestinal Reg I gene expression. | Recent evidence suggests that intestinal Bifidobacterium species (spp.) positively correlates with improved insulin resistance and obesity, and this might be linked to metabolic inflammation. The expression of intestinal REG (regenerating) family proteins which are widely involved in inflammatory bowel disease and diabetes are still unknown in metabolic syndrome. Hence, we investigated the effects of Bifidobacterium longum (BIF) supplementation on metabolic parameters, intestinal function and ex ... | 2011 | 21685239 |
| clinical trial: the microbiological and immunological effects of synbiotic consumption - a randomized double-blind placebo-controlled study in active crohn's disease. | crohn's disease is an inflammatory illness in which the immune response against gut microorganisms is believed to drive an abnormal immune response. consequently, modification of mucosal bacterial communities, and the immune effects they elicit, might be used to modify the disease state. | 2010 | 20735782 |
| the determination of viable counts in probiotic cultures microencapsulated by spray-coating. | an assessment of various methods to determine viable counts (cfu) in freeze-dried and dried microencapsulated (me) probiotic cultures was carried out. microencapsulation was done by spray-coating of dried lactobacillus rhamnosus r0011 or bifidobacterium longum atcc 15708 cultures with fat. rehydration of the me powders was incomplete when they were added to water and gently agitated. as a result analytical methods based on vortexing of rehydrated me cultures and which did not incorporate a high- ... | 2010 | 20832691 |
| Bifidobacterium longum subsp. infantis uses two different {beta}-galactosidases for selectively degrading type-1 and type-2 human milk oligosaccharides. | The breast-fed infant intestine is often colonized by particular bifidobacteria, and human milk oligosaccharides (HMOs) are considered to be bifidogenic. Recent studies showed that Bifidobacterium longum subsp. infantis can grow on HMOs as the sole carbon source. This ability has been ascribed to the presence of a gene cluster (HMO cluster-1) contained in its genome. However, the metabolism of HMOs by the organism remains unresolved because no enzymatic studies have been completed. In the presen ... | 2011 | 21926104 |
| Influence of mother's intestinal microbiota on gut colonization in the infant. | The aim here was to elucidate the mother-infant association in the gut colonization of 1-6 month-old infants and to establish whether probiotics can influence this process. Fecal samples from 80 mother-infant pairs were analyzed at 1 month (mothers and infants) and 6 months (infants) by real-time polymerase chain reaction to assess bacterial numbers. This double-blind placebo-controlled trial involved 2 different probiotic combinations (1. Lactobacillus rhamnosus + Bifidobacterium longum and 2. ... | 2011 | 21983067 |
| Preoperative Probiotics Decrease Postoperative Infectious Complications of Colorectal Cancer. | BACKGROUND: The objective is to elucidate the effects of oral bifid triple viable probiotics among patients with colorectal cancer. METHODS: Sixty patients undergoing radical colorectal resection were randomly assigned to 3-day (days -5 to -3) preoperative probiotics (group A, n = 30) or placebo (group B, n = 30) treatment. The alteration of intestinal flora was evaluated by fecal cultures of Escherichia coli, Bifidobacterium longum and intestinal fungi; the gut barrier function by serum endotox ... | 2011 | 22197980 |
| Novel Probiotic Bifidobacterium longum subsp. infantis CECT 7210 Strain Active against Rotavirus Infections. | Rotavirus is the leading cause of severe acute gastroenteritis among children worldwide. It is well known that breast-feeding and vaccination afford infants protection. Since breast-feeding has drastically decreased in developed countries, efforts have been focused on the potential use of probiotics as preventive agents. In this study, a novel Bifidobacterium longum subsp. infantis strain was isolated from infant feces and selected, based on its capacity to inhibit in vitro rotavirus Wa replicat ... | 2011 | 22003027 |
| changes in ffh, uvra, groes and dnak mrna abundance as a function of acid-adaptation and growth phase in bifidobacterium longum bbmn68 isolated from healthy centenarians. | the acid adaption is commonly used as a strategy to enhance the acid tolerance of bifidobacteria. however, the acid tolerance response (atr) mechanism elicited by this method is unclear. real-time relative-quantitative pcr was applied to analyze the changes in the expressions of ffh, uvra, groes, and dnak involved in the atr after acid-adaptation in bifidobacterium longum bbmn68 in different growth phases. bbmn68 was cultured at a constant neutral ph during the whole growth phase. without acid-a ... | 2011 | 20835829 |
| beneficial psychological effects of a probiotic formulation (lactobacillus helveticus r0052 and bifidobacterium longum r0175) in healthy human volunteers. | | 2011 | 21983070 |
| Effect of the oral intake of yogurt containing Bifidobacterium longum BB536 on the cell numbers of enterotoxigenic Bacteroides fragilis in microbiota. | Enterotoxigenic Bacteroides fragilis (ETBF) strains have been suggested to be associated with acute and persistent diarrheal disease, inflammatory bowel disease and colorectal cancer, although further epidemiological studies are needed for clarification. Here, a pilot study was performed to examine the effect of the oral administration of yogurt supplemented with a probiotic strain on the cell numbers of fecal ETBF in a healthy population. Among 420 healthy adults, 38 subjects were found to be E ... | 2011 | 22138361 |
| examination of faecal bifidobacterium populations in breast- and formula-fed infants during the first 18 months of life. | bifidobacteria in the infant faecal microbiota have been the focus of much interest, especially during the exclusive milk-feeding period and in relation to the fortification of infant formulae to better mimic breast milk. however, longitudinal studies examining the diversity and dynamics of the bifidobacterium population of infants are lacking, particularly in relation to the effects of weaning. using a polyphasic strategy, the bifidobacterium populations of breast- and formula-fed infants were ... | 2010 | 20864478 |
| improved growth of toddlers fed a milk containing synbiotics. | bifidobacterium longum (bl999), lactobacillus rhamonosus (lpr), prebiotics (inulin and fructo-oligosaccharides), and long-chain polyunsaturated fatty acids (lcpufa) are believed to have health benefits. in a randomized, double-blind, controlled trial we compared growth and development of toddlers fed milk containing synbiotics (bl999, lpr, and prebiotics) and lcpufa or a control milk. three hundred and ninety three healthy, 12 month-old toddlers were fed approximately 400 ml/day for 12 months. a ... | 2011 | 21393113 |
| The anxiolytic effect of Bifidobacterium longum NCC3001 involves vagal pathways for gut-brain communication. | The probiotic Bifidobacterium longum NCC3001 normalizes anxiety-like behavior and hippocampal brain derived neurotrophic factor (BDNF) in mice with infectious colitis. Using a model of chemical colitis we test whether the anxiolytic effect of B. longum involves vagal integrity, and changes in neural cell function. Methods Mice received dextran sodium sulfate (DSS, 3%) in drinking water during three 1-week cycles. Bifidobacterium longum or placebo were gavaged daily during the last cycle. Some m ... | 2011 | 21988661 |
| crystal structures of the apo form of ß-fructofuranosidase from bifidobacterium longum and its complex with fructose. | we solved the 1.8 å crystal structure of ß-fructofuranosidase from bifidobacterium longum kn29.1 - a unique enzyme that allows these probiotic bacteria to function in the human digestive system. the sequence of ß-fructofuranosidase classifies it as belonging to the glycoside hydrolase family 32 (gh32). gh32 enzymes show a wide range of substrate specificity and different functions in various organisms. all enzymes from this family share a similar fold, containing two domains: an n-terminal five- ... | 2011 | 21418142 |
| the effect of using different probiotic cultures on conjugated linoleic acid (cla) concentration and fatty acid composition of white pickle cheese. | pickle white cheeses were produced from whole milk with five different probiotic cultures (enterococcus faecium, lactobacillus paracasei, bifidobacterium longum, bifidobacterium bifidum and lactobacillus acidophilus). conjugated linoleic acid (cla) content of cheeses ranged from 3.52 to 3.92 mg/g. probiotic differences and storage process have not affected the cla contents of the samples statistically. there was no correlation between the cla content of all probiotic cheeses and saturated fatty ... | 2011 | 22166042 |
| adhesion of bile-adapted bifidobacterium strains to the ht29-mtx cell line is modified after sequential gastrointestinal challenge simulated in vitro using human gastric and duodenal juices. | according to the fao/who, in vitro criteria for selection of probiotics for food application consist of testing survival when confronted with gastrointestinal tract (git) challenge and the ability to colonize the colon. we used a model that simulated git transit using sequential immersion in gastric and duodenal juices of human origin to evaluate survival of bile-adapted bifidobacterium strains. bifidobacterium animalis tolerated gastric juice, whereas bifidobacterium longum showed poor survival ... | 2011 | 21419219 |
| cloning and characterization of α-l-arabinofuranosidase and bifunctional α-l-arabinopyranosidase/β-d-galactopyranosidase from bifidobacterium longum h-1. | this study focused on the cloning, expression and characterization of recombinant α-l-arabinosidases from bifidobacterium longum h-1. | 2011 | 21851513 |
| the impact of meals on a probiotic during transit through a model of the human upper gastrointestinal tract. | commercial literature on various probiotic products suggests that they can be taken before meals, during meals or after meals or even without meals. this has led to serious confusion for the industry and the consumer. the objective of our study was to examine the impact of the time of administration with respect to mealtime and the impact of the buffering capacity of the food on the survival of probiotic microbes during gastrointestinal transit. we used an in vitro digestive system (ividis) mode ... | 2011 | 22146689 |
| the effect of a multispecies probiotic mixture on the symptoms and fecal microbiota in diarrhea-dominant irritable bowel syndrome: a randomized, double-blind, placebo-controlled trial. | background:: the clinical effect of probiotics on irritable bowel syndrome (ibs) is still controversial. aims:: we aimed to evaluate the effects of a probiotic mixture on ibs symptoms and the composition of fecal microbiota in patients with diarrhea-dominant ibs (d-ibs). methods:: fifty patients with d-ibs were randomized into placebo or probiotic mixture (lactobacillus acidophilus, lactobacillus plantarum, lactobacillus rhamnosus, bifidobacterium breve, bifidobacterium lactis, bifidobacterium l ... | 2011 | 22157240 |
| bifidobacterium longum subsp. infantis atcc 15697 α-fucosidases are active on fucosylated human milk oligosaccharides. | bifidobacterium longum subsp. infantis atcc 15697 utilizes several small-mass neutral human milk oligosaccharides (hmos), several of which are fucosylated. whereas previous studies focused on endpoint consumption, a temporal glycan consumption profile revealed a time-dependent effect. specifically, among preferred hmos, tetraose was favored early in fermentation, with other oligosaccharides consumed slightly later. in order to utilize fucosylated oligosaccharides, atcc 15697 possesses several fu ... | 2011 | 22138995 |
| oligosaccharide binding proteins from bifidobacterium longum subsp. infantis reveal a preference for host glycans. | bifidobacterium longum subsp. infantis (b. infantis) is a common member of the infant intestinal microbiota, and it has been characterized by its foraging capacity for human milk oligosaccharides (hmo). its genome sequence revealed an overabundance of the family 1 of solute binding proteins (f1sbps), part of abc transporters and associated with the import of oligosaccharides. in this study we have used the mammalian glycan array to determine the specific affinities of these proteins. this was co ... | 2011 | 21423604 |
| genetic analysis and morphological identification of pilus-like structures in members of the genus bifidobacterium. | background: cell surface pili in gram positive bacteria have been reported to orchestrate the colonization of host tissues, evasion of immunity and the development of biofilms. so far, little if any information is available on the presence of pilus-like structures in human gut commensals like bifidobacteria. results and discussion: in this report, atomic force microscopy (afm) of various bifidobacterial strains belonging to bifidobacterium bifidum, bifidobacterium longum subsp. longum, bifidobac ... | 2011 | 21995649 |
| role of metabolism by intestinal bacteria in arbutin-induced toxicity in vitro. | a possible role of metabolism by intestinal bacteria in arbutin-induced toxicity was investigated in mammalian cell cultures. following an incubation of arbutin with intestinal bacteria, either bifidobacterium longum hy81 or bifidobacterium adolescentis, for 24 h, its aglycone hydroquinone could be produced and detected in the bacterial culture media. the bacterial growth was not affected up to 10 mm arbutin in the culture medium. when the toxicity of bacteria cultured medium with arbutin was te ... | 2011 | 21544735 |
| The impact of perinatal probiotic intervention on gut microbiota: Double-blind placebo-controlled trials in Finland and Germany. | Specific probiotic combinations during early feeding, via the mother or incorporated in early formula-feeding, mold the intestinal microbiota composition in infants. The objective was to analyze the impact of probiotic administration to mother or infant on gut microbiota composition in 6-month-old Finnish and German infants. In Finland probiotics were given to mothers (n = 79) for 2 months prior to and 2 months after delivery. In Germany probiotics were started in infants (n = 81) at weaning, at ... | 2011 | 21979491 |
| characterization of bifidobacteria suitable for probiotic use in calves. | in our previous experiment, the ten calves originated bifidobacterial strains were administered to calves and re-isolated. fingerprinting techniques used in this study enabled us to distinguish the surviving and non-surviving strains. only the species bifidobacterium animalis ssp. animalis and bifidobacterium longum ssp. suis were found to survive in the intestine. | 2011 | 21986032 |
| proteomics analysis of bifidobacterium longum ncc2705 growing on glucose, fructose, mannose, xylose, ribose, and galactose. | to investigate the molecular mechanisms underlying carbohydrate uptake and connected metabolic pathways of bifidobacterium longum ncc2705, the proteomic profiles of bacteria grown on different carbon sources including glucose, fructose, mannose, xylose, ribose, and galactose were analyzed. our results show that all sugars tested were catabolized via the bifid shunt. sixty-eight proteins that exhibited changes in abundance of threefold or greater were identified by ms. a striking observation was ... | 2011 | 21630463 |
| Pediatric functional constipation treatment with Bifidobacterium-containing yogurt: a crossover, double-blind, controlled trial. | To evaluate the treatment of pediatric functional chronic intestinal constipation (FCIC) with a probiotic goat yogurt. | 2011 | 22025880 |
| Comparative effects of six probiotic strains on immune function in vitro. | There is considerable interest in the strain specificity of immune modulation by probiotics. The present study compared the immunomodulatory properties of six probiotic strains of different species and two genera in a human peripheral blood mononuclear cell (PBMC) model in vitro. Live cells of lactobacilli (Lactobacillus casei Shirota, L. rhamnosus GG, L. plantarum NCIMB 8826 and L. reuteri NCIMB 11951) and bifidobacteria (Bifidobacterium longum SP 07/3 and B. bifidum MF 20/5) were individually ... | 2011 | 22054064 |
| Encapsulation of probiotic Bifidobacterium longum BIOMA 5920 with alginate-human-like collagen and evaluation of survival in simulated gastrointestinal conditions. | Alginate (ALg)-human-like collagen (HLC) microspheres were prepared by the technology of electrostatic droplet generation in order to develop a biocompatible vehicle for probiotic bacteria. Microparticles were spherical with mean particle size of 400µm. The encapsulation efficiency (EE) of ALg-HLC microspheres could reach 92-99.2%. Water-soluble and fibrous human-like collagen is combined with sodium alginate through intermolecular hydrogen bonding and electrostatic force which were investigated ... | 2011 | 21889530 |
| fructose uptake in bifidobacterium longum ncc2705 is mediated by an atp-binding cassette transporter. | recently, a putative atp-binding cassette (abc) transport system was identified in bifidobacterium longum ncc2705 that is highly up-regulated during growth on fructose as the sole carbon source. cloning and expression of the corresponding orfs (bl0033-0036) result in efficient fructose uptake by bacteria. sequence analysis reveals high similarity to typical abc transport systems and suggests that these genes are organized as an operon. expression of frue is induced by fructose, ribose, or xylose ... | 2012 | 22102285 |