| detection of human metapneumovirus antigens in nasopharyngeal aspirates using an enzyme immunoassay. | human metapneumovirus (hmpv) is associated with acute respiratory tract infections (arti) in patients from all age groups. | 2009 | 19010729 |
| infection with human metapneumovirus predisposes mice to severe pneumococcal pneumonia. | human metapneumovirus (hmpv) is a recently described paramyxovirus that causes respiratory tract infections. prior clinical studies have highlighted the importance of respiratory viruses, such as influenza virus, in facilitating secondary bacterial infections and increasing host immunopathology. the objective of the present work was to evaluate the effects of initial viral infection with hmpv or influenza a virus followed by streptococcus pneumoniae superinfection 5 days later in a murine model. ... | 2009 | 19019962 |
| clinical characterization of human metapneumovirus infection among patients with cancer. | human metapneumovirus is a recently discovered rna virus that typically causes respiratory disease in children. it has been linked to severe lower airway disease in hematopoietic stem cell and solid-organ transplant recipients. hmpv infection in a large population of patients with underlying cancer and varying degrees of immunosuppression has not been reported. we sought to characterize hmpv infection in patients with cancer. | 2008 | 19027169 |
| low-ph triggering of human metapneumovirus fusion: essential residues and importance in entry. | human metapneumovirus (hmpv) is a significant respiratory pathogen classified in the pneumovirinae subfamily of the paramyxovirus family. recently, we demonstrated that hmpv f protein-promoted cell-cell fusion is stimulated by exposure to low ph, in contrast to what is observed for other paramyxovirus f proteins. in the present study, we examined the potential role of histidine protonation in hmpv f fusion and investigated the role of low ph in hmpv viral entry. mutagenesis of the three ectodoma ... | 2009 | 19036821 |
| prevalence, clinical presentations and complications among hospitalized children with influenza pneumonia. | viral respiratory tract infections are a major cause of hospitalization in children. influenza is common but often not laboratory proven. we report a prospective study of children admitted with a clinical diagnosis of pneumonia. infants and children (ages 1 month-15 years) who were hospitalized with community-acquired pneumonia were enrolled in the study. their nasopharyngeal aspirated samples were analyzed for common respiratory viruses, including influenza virus, by reverse transcription-polym ... | 2008 | 19050351 |
| evaluation of real-time rt-pcr compared with conventional rt-pcr for detecting human metapneumovirus rna from clinical specimens. | human metapneumovirus (hmpv) is an etiologic agent of respiratory tract infections. in this study, we compared the sensitivity and specificity of real-time reverse transcription (rt)-polymerase chain reaction (pcr), conventional rt-pcr, and nested pcr in detecting hmpv genes. a total of 146 clinical specimens from 143 patients who showed acute respiratory tract infection symptoms were tested by real-time rt-pcr, conventional rt-pcr, and nested pcr targeting for the fusion gene. we detected hmpv ... | 2008 | 19050355 |
| human metapneumovirus infection in febrile children with lower respiratory diseases in primary care settings in hiroshima, japan. | human metapneumovirus (hmpv) has been shown to be a leading cause of viral lower respiratory tract infections in children. nevertheless, few reports regarding hmpv infections over consecutive years in children in primary care settings are available. we carried out virologic and clinical studies to determine the role of hmpv in febrile lower respiratory infections in children at a primary care clinic over 3 years and 5 months. nasopharyngeal aspirates obtained from children with acute respiratory ... | 2008 | 19050367 |
| induced sputum in the diagnosis of childhood community-acquired pneumonia. | the usefulness of induced sputum in searching for causative agents of pneumonia in children has not been studied. | 2009 | 19052043 |
| rapid detection and identification of 12 respiratory viruses using a dual priming oligonucleotide system-based multiplex pcr assay. | acute viral respiratory infections are among the most common causes of human disease. rapid and accurate diagnosis of viral respiratory infections is important for providing timely therapeutic interventions. this study evaluated a new multiplex pcr assay (seegene inc., seoul, korea) for simultaneous detection and identification of 12 respiratory viruses using two primer mixes. the viruses included parainfluenza viruses 1, 2, and 3, human metapneumovirus, human coronavirus 229e/nl63 and oc43, ade ... | 2009 | 19063921 |
| human metapneumovirus infections in adults: another piece of the puzzle. | each winter respiratory viruses account for a significant proportion of serious respiratory illness, including hospitalization, in older adults and those with underlying medical conditions. we describe the incidence and clinical impact of human metapneumovirus (hmpv), a newly identified virus, in adults. | 2008 | 19064834 |
| high seroprevalence of human metapneumovirus infection in children in chongqing, china. | the human metapneumovirus (hmpv) is a newly discovered respiratory viral pathogen that was first isolated in 2001 in the netherlands. its global distribution and long history of infection in humans have been well documented. in this study, we assessed the seropositivity of hmpv igg antibodies in children in chongqing, china. | 2008 | 19080178 |
| characterization of human metapneumoviruses isolated in chongqing, china. | human metapneumovirus (hmpv) has recently been recognized as a notable respiratory pathogen in children. however, no isolation processes and only a limited understanding of hmpv epidemiology present in chinese children are documented by far. | 2008 | 19080329 |
| comprehensive detection of causative pathogens using real-time pcr to diagnose pediatric community-acquired pneumonia. | we have developed a real-time reverse transcription-pcr (rt-pcr) method to detect 13 respiratory viruses: influenza virus a and b; respiratory syncytial virus (rsv) subgroup a and b; parainfluenza virus (piv) 1, 2, and 3; adenovirus; rhinovirus (rv); enterovirus; coronavirus (oc43); human metapneumovirus (hmpv); and human bocavirus (hbov). the new method for detection of these viruses was applied simultaneously with real-time pcr for the detection of six bacterial pathogens in clinical samples f ... | 2008 | 19089556 |
| genetic variability of human metapneumovirus isolated from chilean children, 2003-2004. | human metapneumovirus (hmpv) is a significant cause of acute lower respiratory tract infection in all age groups, particularly in children. two genetic groups and four subgroups of hmpv have been described. they co-circulate during an epidemic in variable proportions. the aims were to characterize the genotypes of hmpv recovered from children hospitalized for acute lower respiratory tract infection and to establish the molecular epidemiology of strains circulating in santiago of chile during a 2 ... | 2009 | 19107972 |
| infection and maturation of monocyte-derived human dendritic cells by human respiratory syncytial virus, human metapneumovirus, and human parainfluenza virus type 3. | human respiratory syncytial virus (hrsv), human metapneumovirus (hmpv), and human parainfluenza virus type 3 (hpiv3) are common, important respiratory pathogens, but hrsv has a substantially greater impact with regard to acute disease, long-term effects on airway function, and frequency of re-infection. it has been reported to strongly interfere with the functioning of dendritic cells (dc). we compared hrsv to hmpv and hpiv3 with regard to their effects on human monocyte-derived immature dc (idc ... | 2009 | 19128816 |
| human bocavirus respiratory infections in children. | human bocavirus (hbov) was recently identified in respiratory samples from patients with acute respiratory infections and has been reported in different regions of the world. to the best of our knowledge, hbov has never been reported in respiratory infections in brazil. nasopharyngeal aspirates were collected from patients aged <5 years hospitalized in 2005 with respiratory infections in ribeirão preto, southeast brazil, and tested by polymerase chain reaction (pcr) for hbov. hbov-positive sampl ... | 2009 | 19134237 |
| age-associated aggravation of clinical disease after primary metapneumovirus infection of balb/c mice. | human metapneumovirus (hmpv) is associated with respiratory tract infections among children and adults. because hmpv induces significant morbidity and mortality in the elderly, a model of hmpv infection in aged balb/c mice was established. young (8 weeks old) and aged (18 months old) mice were intranasally inoculated with hmpv. the infected mice showed respiratory dysfunction, as measured by plethysmography, a marked loss in weight (up to 24%), and severe histopathological abnormalities includin ... | 2009 | 19144706 |
| comparison of automated microarray detection with real-time pcr assays for detection of respiratory viruses in specimens obtained from children. | respiratory virus infections are a major health concern and represent the primary cause of testing consultation and hospitalization for young children. we developed and compared two assays that allow the detection of up to 23 different respiratory viruses that frequently infect children. the first method consisted of single taqman quantitative real-time pcr assays in a 96-well-plate format. the second consisted of a multiplex pcr followed by primer extension and microarray hybridization in an in ... | 2009 | 19158263 |
| integrin alphavbeta1 promotes infection by human metapneumovirus. | human metapneumovirus (hmpv) is a recently described paramyxovirus that causes lower respiratory infections in children and adults worldwide. the hmpv fusion (f) protein is a membrane-anchored glycoprotein and major protective antigen. all hmpv f protein sequences determined to date contain an arg-gly-asp (rgd) sequence, suggesting that f engages rgd-binding integrins to mediate cell entry. the divalent cation chelator edta, which disrupts heterodimeric integrin interactions, inhibits infectivit ... | 2009 | 19164533 |
| association between seroprevalence of human metapneumovirus and c-reactive protein level and apolipoprotein e-epsilon4 allele in elderly inpatients in japan. | | 2008 | 19166449 |
| respiratory viruses in bronchoalveolar lavage: a hospital-based cohort study in adults. | the epidemiology of respiratory viruses and their potential clinical impact when recovered in lower respiratory specimens has not been established in the hospital setting. a study was performed to investigate the association between positive viral detection and respiratory infection in an at-risk population. | 2009 | 19174425 |
| human metapneumovirus and human bocavirus in children. | several new viruses have recently been described in children, including human metapneumovirus (hmpv) and human bocavirus (hbov). hmpv has been established as a common cause of upper and lower respiratory tract infections in children, often second only to respiratory syncytial virus as a cause of bronchiolitis in infants. diagnostic tools have been developed for the clinician and effective treatment and prevention strategies are being investigated. hbov was more recently identified. although it w ... | 2009 | 19190535 |
| establishing a surveillance network for severe lower respiratory tract infections in korean infants and young children. | to reduce morbidity and mortality through integrated case management, a pilot study to detect respiratory viruses in patients with acute lower respiratory infections (alris) was designed as part of a nationwide surveillance for this disease in korea. the study population consisted of hospitalized patients under the age of 5 years with bronchiolitis, pneumonia, croup, or acute respiratory distress syndrome. a prospective 6-month study was performed. two hundred and ninety-seven nasopharyngeal sec ... | 2009 | 19190941 |
| healthcare-associated atypical pneumonia. | atypical pneumonia was first described in 1938, and over time, mycoplasma, legionella, and chlamydophila were the agents commonly linked with community-associated atypical pneumonia. however, as technology has improved, so has our understanding of this clinical entity. it is now known that there are many agents linked with atypical pneumonia in the community, and many of these agents are also major causes of healthcare-associated pneumonia. this article discusses the history, epidemiology, and p ... | 2009 | 19199189 |
| human metapneumovirus: a new respiratory pathogen. | human metapneumovirus is a recently recognized pathogen of acute respiratory tract infection (ari) in children as well as elderly and immunocompromised adults.the virus belongs to the family paramyxoviridae, sub family pneumovirinae and genus metapneumovirus. through genetic analysis it has been characterized into two groups a and b which are further divided into four sub-lineages. the virus is difficult to grow in tissue culture and hence reverse transcriptase-polymerase chain reaction (rt-pcr) ... | 2008 | 19208974 |
| low prevalence of human metapneumovirus and human bocavirus in adult immunocompromised high risk patients suspected to suffer from pneumocystis pneumonia. | novel respiratory viruses were discovered in the last years predominantly in children. until now information on newly identified respiratory viruses in immunosuppressed adult patients is limited. here we investigated immunocompromised adults with suspected pneumocystis jirovecii pneumonia (pcp) for new respiratory viruses. | 2009 | 19211148 |
| molecular epidemiological investigation of a nosocomial outbreak of human metapneumovirus infection in a pediatric hemato-oncology patient population. | we report the first nosocomial outbreak of human metapneumovirus (hmpv) infection in a pediatric patient population. among 15 pediatric hmpv infections from march to may 2007, there was a nosocomial outbreak involving two patients sharing a room in a hemato-oncology ward with a patient with a community-acquired case of hmpv infection. the estimated incubation period was 7 to 9 days for a symptomatic nosocomial case. sequencing of fusion genes of 15 isolates revealed two clusters belonging to sub ... | 2009 | 19213698 |
| micronutrient concentrations in respiratory syncytial virus and human metapneumovirus in yemeni children. | acute respiratory infections (ari) cause significant childhood mortality. nutritional homeostasis, particularly micronutrient levels, is important in modulating response to infection. more information is required regarding micronutrient levels in ari viral infections, especially newly identified viruses such as human metapneumovirus (hmpv). | 2009 | 19222932 |
| subversion of pulmonary dendritic cell function by paramyxovirus infections. | lower respiratory tract infections caused by the paramyxoviruses human metapneumovirus (hmpv) and respiratory syncytial virus (rsv) are characterized by short-lasting virus-specific immunity and often long-term airway morbidity, both of which may be the result of alterations in the ag-presenting function of the lung which follow these infections. in this study, we investigated whether hmpv and rsv experimental infections alter the phenotype and function of dendritic cell (dc) subsets that are re ... | 2009 | 19234204 |
| epidemiological and clinical study of viral respiratory tract infections in children from italy. | impact of recently discovered viruses on epidemiology of acute respiratory tract infections (arti) is still unclear. we studied the impact of recently discovered human metapneumovirus (hmpv), human bocavirus (hbov), and new coronaviruses (hcov-nl63 and hku1) on the global epidemiology of arti. from october 2006 to april 2007, 237 pediatric patients affected by arti were enrolled in our study. specimens were tested for respiratory viruses by polymerase chain reaction. one hundred twenty-four out ... | 2009 | 19235872 |
| human metapneumovirus infections--biannual epidemics and clinical findings in children in the region of basel, switzerland. | human metapneumovirus (hmpv) epidemics vary in time and severity. we report findings for pcr for hmpv and respiratory syncytial virus (rsv) performed on nasopharyngeal aspirates (npa) of hospitalized and outpatient children with respiratory tract infections between october 2004 and april 2008. a total of 3,934 npas were tested for hmpv and 3,859 for rsv. of these, 198 (5%) were hmpv positive and 869 (23%) were rsv-positive. median age was 17 months and 9 months for hmpv and rsv, respectively. fi ... | 2009 | 19238433 |
| in very young infants severity of acute bronchiolitis depends on carried viruses. | rt amplification reaction has revealed that various single viruses or viral co-infections caused acute bronchiolitis in infants, and rv appeared to have a growing involvement in early respiratory diseases. because remaining controversial, the objective was to determine prospectively the respective role of rsv, rv, hmpv and co-infections on the severity of acute bronchiolitis in very young infants. | 2009 | 19240806 |
| use of a universal virus detection assay to identify human metapneumovirus in a hematopoietic stem cell transplant recipient with pneumonia of unknown origin. | development of uncommon viral infections in immunocompromised transplant recipients can pose major diagnostic challenges. we present a case report of an immunocompromised patient suffering from pneumonia, for which the causative agent was not identified by routine methods. | 2009 | 19250860 |
| fusion protein is the main determinant of metapneumovirus host tropism. | human metapneumovirus (hmpv) and avian metapneumovirus subgroup c (ampv-c) infect humans and birds, respectively. this study confirmed the difference in host range in turkey poults, and analysed the contribution of the individual metapneumovirus genes to host range in an in vitro cell-culture model. mammalian vero-118 cells supported replication of both hmpv and ampv-c in contrast to avian quail fibroblast (qt6) cells in which only ampv-c replicated to high titres. inoculation of vero-118 and qt ... | 2009 | 19264630 |
| aetiology of influenza-like illness in adults includes parainfluenzavirus type 4. | influenza viruses cause significant morbidity and mortality in adults each winter. at the same time, other respiratory viruses circulate and cause respiratory illness with influenza-like symptoms. human respiratory syncytial virus (hrsv), human parainfluenza viruses (hpiv) and human metapneumovirus have all been associated with morbidity and mortality in adults, including nosocomial infections. this study evaluated 154 respiratory specimens collected from adults with influenza-like/acute respira ... | 2009 | 19273634 |
| [viral pathogens of acute lower respiratory tract infection in hospitalized children from east guangdong of china]. | to investigate the viral pathogens of acute lower respiratory tract infection (alrti) in hospitalized children from east guangdong province of china and the relationship of the pathogens with age and seasons. | 2009 | 19292960 |
| human metapneumovirus in costa rica. | | 2009 | 19295458 |
| an outbreak of human metapneumovirus infection in hospitalized psychiatric adult patients in taiwan. | human metapneumovirus (hmpv) is a paramyxovirus that is associated with respiratory tract infection (rti) mostly in children, but these outbreaks have rarely been reported in adults. we encountered an outbreak of this disease involving 10 adults in a psychiatric ward in eastern taiwan. the nasopharyngeal swab specimens from 13 patients with symptoms of rti were obtained and analyzed. the rt-pcr tests were negative to influenza virus a/b, adenovirus, rsv, parainfluenza virus, coronavirus, nipah v ... | 2009 | 19308801 |
| epidemiology and genetic variability of human metapneumovirus during a 4-year-long study in southeastern brazil. | epidemiological and molecular characteristics of human metapneumovirus (hmpv) were compared with human respiratory syncytial virus (hrsv) in infants and young children admitted for acute lower respiratory tract infections in a prospective study during four consecutive years in subtropical brazil. genescan polymerase chain assays (genescan rt-pcr) were used to detect hmpv and hrsv in nasopharyngeal aspirates of 1,670 children during january 2003 to december 2006. hmpv and hrsv were detected, resp ... | 2009 | 19319957 |
| successful treatment of human metapneumovirus pneumonia using combination therapy with intravenous ribavirin and immune globulin. | | 2009 | 19344395 |
| human metapneumovirus establishes persistent infection in the lungs of mice and is reactivated by glucocorticoid treatment. | human metapneumovirus (hmpv) has been identified as a worldwide agent of serious upper and lower respiratory tract infections in infants and young children. hmpv is second only to respiratory syncytial virus (rsv) as a leading cause of bronchiolitis, and, like rsv, consists of two major genotypes that cocirculate and vary among communities year to year. children who have experienced acute hmpv infection may develop sequelae of wheezing and asthma; however, the features contributing to this patho ... | 2009 | 19357175 |
| development of an assay for the detection and quantification of the measles virus nucleoprotein (n) gene using real-time reverse transcriptase pcr. | we developed a new quantification method for the measles virus (mev) nucleoprotein (n) gene using real-time reverse transcriptase pcr. this method allowed us to quantify 10(1)-10(7) copies per reaction (corresponding to 5x10(-1)-5x10(5) copies microl(-1)) of the mev n gene. we also quantified the mev n gene from the throat swabs of 22 patients with measles as well as the mev genotypes a, d3, d5, d9 and h1 in viral suspensions derived from mev-infected cells. as a result, 3.9x10(3)-5.2x10(6) copi ... | 2009 | 19369526 |
| detection of respiratory viruses with a multiplex polymerase chain reaction assay (multicode-plx respiratory virus panel) in patients with hematologic malignancies. | respiratory viral pathogens are a common cause of morbidity in patients with hematologic malignancies. sensitive molecular assays have increased the detection of common respiratory viruses and expanded the panel of detectable viruses. both a rapid viral culture with direct fluorescence antibody (dfa) staining and a pcr-based assay (multicode-plx respiratory virus panel) were performed on patients with hematologic malignancies, who underwent collection of a nasopharyngeal swab or bronchoalveolar ... | 2009 | 19373660 |
| bugs and asthma: a different disease? | the prevalence of asthma has dramatically increased in recent decades. exacerbations of asthma are a large contributor to asthma-related costs, and are primarily caused by viral and atypical bacterial infections. rhinoviruses (rvs) are the most common viruses detected after an asthma exacerbation. rvs, respiratory syncytial virus (rsv), and human metapneumovirus (hmpv) viral infections early in life can induce wheezing and are associated with the development of asthma later in life. atypical bac ... | 2009 | 19387028 |
| the cotton rat model of respiratory viral infections. | development of successful vaccines against human infectious diseases depends on using appropriate animal models for testing vaccine efficacy and safety. for some viral infections the task is further complicated by the frequently changing genetic make-up of the virus, as in the case of influenza, or by the existence of the little-understood phenomenon of vaccine-enhanced disease, as in the case of respiratory syncytial virus (rsv). the cotton rat sigmodon hispidus has been used for years as an ex ... | 2009 | 19394861 |
| novel respiratory virus infections in children, brazil. | recently discovered respiratory viruses were detected in 19 (9.2%) of 205 nasal swab specimens from children in brazil with respiratory illnesses. five each were positive for human metapneumovirus (hmpv) alone and human bocavirus (hbov) alone, 3 for human coronaviruses (hcov-hku1 or -nl63) alone, and 6 for more than 1 recently discovered virus. | 2009 | 19402976 |
| [a comparative study of direct immunofluorescence, enzyme immunoassay, and culture for diagnosing metapneumovirus infection]. | human metapneumovirus (hmpv) is an important cause of lower respiratory tract infections in children, accounting for 14% to 24% of all viral respiratory infections with an etiological diagnosis. this study compares a direct fluorescent antibody (dfa) test, enzyme immunoassay (eia), and shell-vial culture for diagnosing acute bronchiolitis in infants. | 2009 | 19423194 |
| review of new and newly discovered respiratory tract viruses in children. | respiratory tract viral infection continues to be among the most common reasons for emergency department visits and hospitalization of children, particularly infants younger than 1 year, in the united states. throughout the years, clinicians have considered respiratory syncytial virus followed by influenza as the most common pathogens responsible. over the past decade, new viruses have been discovered through both more specific testing and the finding of new agents causing infection. this includ ... | 2009 | 19444037 |
| epidemiological study of influenza virus infections in young adult outpatients from buenos aires, argentina. | influenza virus is the most common cause of influenza-like illness (ili) in adults. in argentina, studies on influenza and other respiratory viruses were performed mostly in pediatric populations. | 2008 | 19453464 |
| human coronavirus nl63 infections in infants hospitalised with acute respiratory tract infections in south africa. | human coronavirus nl63 (hcov-nl63) is a novel respiratory virus which is associated with respiratory tract infections in children. | 2008 | 19453465 |
| comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients. | sensitive detection of respiratory viruses is important for early diagnosis of infection in patients following hematopoietic cell transplantation (hct). to evaluate the relative sensitivity of respiratory virus detection in specimens from hct recipients, we compared the results of conventional and quantitative molecular methods. | 2009 | 19453994 |
| prevalence of human respiratory viruses in adults with acute respiratory tract infections in beijing, 2005-2007. | to determine the aetiological role and epidemiological profile of common respiratory viruses in adults with acute respiratory tract infections (artis), a 2-year study was conducted in beijing, china, from may 2005 to july 2007. nose and throat swab samples from 5808 arti patients were analysed by pcr methods for common respiratory viruses, including influenza viruses (ifvs) a, b, and c, parainfluenza viruses (pivs) 1-4, enteroviruses (evs), human rhinoviruses (hrvs), respiratory syncytial virus ... | 2009 | 19456830 |
| human metapneumovirus: a new threat? | | 2009 | 19478119 |
| rapid detection of respiratory picornaviruses in nasopharyngeal aspirates by immunofluorescence assay. | respiratory picornaviruses (enteroviruses and rhinoviruses) are commonly cited as causes of self-limited upper respiratory tract infection. however, it has recently been suggested that they may cause more severe respiratory disease. immunofluorescence (if) assays are rapid and inexpensive and are often used for the detection of respiratory viruses. | 2009 | 19502108 |
| application of rt-pcr for diagnosis of respiratory syncytial virus and human metapneumovirus infections in bulgaria, 2006-7 and 2007-8. | we describe here the results of respiratory syncytial virus (rsv) detection by reverse transcription polymerase chain reaction (rt-pcr) during two consecutive seasons, from december 2006 to february 2007 and from october 2007 to march 2008, performed in the national laboratory of influenza and acute respiratory diseases, bulgaria. a total number of 278 nasopharyngeal samples obtained from hospitalised children up to the age of five years were investigated for these two seasons. during the first ... | 2009 | 19531340 |
| [investigation of human metapneumovirus in children with acute respiratory tract infections in guangzhou areas]. | to find out the status of human metapneumovirus (hmpv) infection in children under 14 years old with acute respiratory tract infections (ari) in guangzhou, analyze the epidemiology and clinical characteristics among the hmpv-infected children, and provide some basis for research of hmpv. | 2009 | 19534954 |
| [detection and identification of human metapneumovirus by real time reverse transcription pcr]. | to develop a rapid, sensitive and specific real time reverse transcription pcr for detecting and identifying human metapneumovirus. | 2008 | 19544662 |
| analysis of antigenic cross-reactivity between subgroup c avian pneumovirus and human metapneumovirus by using recombinant fusion proteins. | avian pneumovirus subgroup c (apv/c) has recently been reported to be more closely related to human metapneumovirus (hmpv) as determined by sequence analysis. to examine the antigenic relationship between apv/c and hmpv, the apv/c fusion (f) gene was cloned and expressed as an uncleaved glycoprotein in a baculovirus system. the reactivity of the apv/c f protein with antibodies against apv subgroups a, b, c, and hmpv was examined by western blot analysis. the results showed that the expressed apv ... | 2009 | 19548897 |
| [detection of human metapneumovirus and human bocavirus in children with bronchiolitis in east guangdong area]. | | 2009 | 19555574 |
| respiratory viral infections detected by multiplex pcr among pediatric patients with lower respiratory tract infections seen at an urban hospital in delhi from 2005 to 2007. | acute lower respiratory tract infections (alri) are the major cause of morbidity and mortality in young children worldwide. information on viral etiology in alri from india is limited. the aim of the present study was to develop a simple, sensitive, specific and cost effective multiplex pcr (mpcr) assay without post pcr hybridization or nested pcr steps for the detection of respiratory syncytial virus (rsv), influenza viruses, parainfluenza viruses (piv1-3) and human metapneumovirus (hmpv). naso ... | 2009 | 19558656 |
| evaluation of a new rapid antigen test using immunochromatography for detection of human metapneumovirus in comparison with real-time pcr assay. | a new rapid human metapneumovirus (hmpv) detection kit using immunochromatography (sas hmpv test) was compared to real-time pcr for 224 nasal swab specimens, 96.4% of which were obtained from children of <15 years of age. the overall sensitivity and specificity were 82.3% and 93.8%, respectively, suggesting that this test is useful for pediatricians to diagnose hmpv infection in a clinical setting. | 2009 | 19571031 |
| the role of toll pathways in viral respiratory disease during early childhood. | virus-induced respiratory disease accounts for the majority of hospitalizations of young children during the winter months. the major viral causes of lower respiratory tract disease in infants and children are respiratory syncytial virus (rsv), rhinovirus, metapneumovirus (hmpv), parainfluenza virus 3 (piv-3), and influenza. the primary antiviral mechanism involves the activation of cytotoxic t cells and the clearance of virus-infected epithelial cells, and toll-like receptors that recognize mic ... | 2009 | 19601804 |
| outbreak of human metapneumovirus infection in a residential aged care facility. | summer outbreaks of respiratory illness in residential aged care facilities are uncommonly reported in new south wales. a respiratory illness outbreak in an aged care facility during january 2008 prompted a response to contain the outbreak by implementing infection control measures, including cohorting of symptomatic residents, cohorting nursing care, closure to new admissions and the use of personal protective equipment by staff. in addition, respiratory tract specimens were collected to determ ... | 2009 | 19618769 |
| exhalation of respiratory viruses by breathing, coughing, and talking. | there is a lack of quantitative information about the generation of virus aerosols by infected subjects. the exhaled aerosols generated by coughing, talking, and breathing were sampled in 50 subjects using a novel mask, and analyzed using pcr for nine respiratory viruses. the exhaled samples from a subset of 10 subjects who were pcr positive for rhinovirus were also examined by cell culture for this virus. of the 50 subjects, among the 33 with symptoms of upper respiratory tract infections, 21 h ... | 2009 | 19626609 |
| rna viruses in community-acquired childhood pneumonia in semi-urban nepal; a cross-sectional study. | pneumonia is among the main causes of illness and death in children <5 years of age. there is a need to better describe the epidemiology of viral community-acquired pneumonia (cap) in developing countries. | 2009 | 19635124 |
| simultaneous detection of respiratory viruses in children with acute respiratory infection using two different multiplex reverse transcription-pcr assays. | a 4-tube multiplex rt-pcr (mrt-pcr), which showed higher sensitivity over conventional methods, was previously developed for the diagnosis of 14 viral pathogens of the respiratory tract. herein the mrt-pcr was compared to the commercial luminex mpcr-microsphere flow cytometry assay (resplex ii) which allows the detection of 12 different viruses. eleven different viruses were identified in 91 nasopharyngeal swabs of children with acute respiratory infection, influenza a (iav) and b, respiratory s ... | 2009 | 19638288 |
| [viral pathogens of acute respiratory infection in hospitalized children from suzhou]. | to investigate the viral pathogens of acute respiratory infection (ari) in hospitalized children from suzhou of china. | 2009 | 19650982 |
| the year in review. | over the last year there have been more studies determining predisposition to severe bronchiolitis and its consequences. studies have highlighted various single-nucleotide polymorphisms (snps) to be significantly associated with respiratory syncytial virus (rsv) hospitalisation, and a candidate gene approach demonstrated that innate immune gene snps had the strongest association with bronchiolitis. the impact of 'other' viruses (rsv, influenza, adenovirus, parainfluenza, rhinovirus, human metapn ... | 2009 | 19651389 |
| human metapneumovirus in children with acute respiratory tract infections in suzhou, china 2005-2006. | this study was conducted to determine the seasonal distribution of human metapneumovirus (hmpv) infection in the suzhou area of china, to discuss the epidemiological and clinical features of the disease, and to examine the genetic variation of the virus in china. from november 2005 to october 2006, a total of 1932 children with acute respiratory infections, admitted to the children's hospital affiliated to soochow university, were tested for the presence of hmpv using a reverse-transcription pol ... | 2009 | 19685378 |
| detection of all four human metapneumovirus subtypes in nasopharyngeal specimens from children with respiratory disease in uberlândia, brazil. | the human metapneumovirus (hmpv) is a pathogen of the respiratory tract identified first in the netherlands in 2001 and since then it has been detected worldwide. the purpose of this study was to identify and characterize hmpv in samples collected from children <5 years presenting with acute respiratory disease (ard) seen at a public hospital in uberlândia, in southeastern brazil. one hundred fourteen nasopharyngeal aspirates (npas) samples that were negative for the presence of nine other respi ... | 2009 | 19697417 |
| simultaneous detection of human bocavirus and adenovirus by multiplex real-time pcr in a belgian paediatric population. | since the discovery of human bocavirus (hbov), the virus has been detected worldwide in respiratory tract samples from young children by various polymerase chain reaction (pcr) assays and real-time pcrs (q-pcr). until now, no data have been reported on the presence of hbov in belgium and the detection of hbov in a multiplex q-pcr setting has not been described. the aim of this study was to develop a fast and reliable multiplex q-pcr for the simultaneous detection of hbov dna and adenovirus (adv) ... | 2009 | 19705175 |
| comparison of human metapneumovirus infection with respiratory syncytial virus infection in children. | we aimed to validate a direct immunofluorescence assay (dfa) for the detection of human metapneumovirus (hmpv) from nasal swabs and to determine the incidence and clinical features of this viral infection in a pediatric population. one hundred twenty-one of 3026 nasal swabs were positive for hmpv by dfa (4.0%). compared with reverse transcriptase polymerase chain reaction, the sensitivity and specificity of dfa were 90%, and 100%, respectively. compared with rsv, hmpv infection was more common i ... | 2009 | 19730154 |
| human metapneumovirus and other respiratory viral infections in children attending a day care center. | human metapneumovirus (hmpv) is an important cause of acute respiratory infections (ari). we studied 335 ari episodes in 74 children<5 years of age attending a day care center during a 1-year period to detect the presence of hmpv and other viruses. seven (9.5%) children were infected by hmpv and this virus accounted for 2.1% of the ari episodes. | 2009 | 19738509 |
| genetic diversity and evolution of human metapneumovirus fusion protein over twenty years. | human metapneumovirus (hmpv) is an important cause of acute respiratory illness in children. we examined the diversity and molecular evolution of hmpv using 85 full-length f (fusion) gene sequences collected over a 20-year period. | 2009 | 19740442 |
| human metapneumovirus associated with central nervous system infection in children. | human metapneumovirus (hmpv) is an established pathogen of the respiratory tract of children and adults. hmpv is related to other paramyxoviruses known to cause encephalitis. reports suggest that hmpv may cause disease of the central nervous system (cns). | 2009 | 19755929 |
| prevalence of human metapneumovirus in hospitalized children with respiratory tract infections in tianjin, china. | human metapneumovirus (hmpv) has recently been recognized as an important respiratory pathogen, especially in children. at present, our understanding of the characteristics of hmpv from china is very limited. nasopharyngeal aspirates were taken from 310 hospitalized pediatric patients. twenty (6.5%) of them were infected with hmpv, and they all developed pneumonia. sixty five percent (13/20) of the cases were under 12 months. phylogenetic analysis of f gene fragments indicated that three sub-gen ... | 2009 | 19774338 |
| association of human metapneumovirus with radiologically diagnosed community-acquired alveolar pneumonia in young children. | to determine the involvement of human metapneumovirus (hmpv) in childhood community-acquired alveolar pneumonia (caap) and compare the demographic, clinical, and laboratory features of hmpv-associated caap and caap associated with other respiratory viruses. | 2010 | 19782998 |
| human hepg2 cells support respiratory syncytial virus and human metapneumovirus replication. | human metapneumovirus (hmpv) and human respiratory syncytial (rsv) virus cause mild to severe infections of the respiratory tract in all age groups. so far, several cell lines derived from respiratory tissues have been identified to support replication of both viruses. unfortunately, titers attained during replication differ between the both viruses within one cell line despite equal infection conditions, on the one hand giving raise to the assumption that the individual susceptibility may vary ... | 2010 | 19799934 |
| microneutralization assay for the measurement of neutralizing antibodies to human metapneumovirus. | human metapneumovirus (hmpv) is a newly discovered virus which causes respiratory illness in persons of all ages. | 2009 | 19818678 |
| incidence, molecular epidemiology and clinical presentations of human metapneumovirus; assessment of its importance as a diagnostic screening target. | human metapneumovirus (hmpv) is a recently discovered human paramyxovirus associated with a spectrum of respiratory symptoms from the common cold to pneumonia and bronchiolitis. | 2009 | 19819755 |
| respiratory syncytial virus, human bocavirus and rhinovirus bronchiolitis in infants. | to investigate the prevalence of 14 viruses in infants with bronchiolitis and to study demographic and clinical differences in those with respiratory syncytial virus (rsv), human bocavirus (hbov) and rhinovirus (rv) infection. | 2010 | 19822538 |
| human metapneumovirus infection in a cohort of young asymptomatic subjects. | human metapneumovirus (hmpv) is a recently described cause of respiratory infection. the purpose of this study was to to further the available information on the circulation of hmpv among young asymptomatic subjects. the epidemiology of hmpv was examined in a cohort of 73 university students during a winter season by using polymerase chain reaction. hpmv was detected in 3/73 (4.1%) asymptomatic subjects. phylogenetic analysis has shown that circulating viruses belonged to different hmpv sublinea ... | 2009 | 19845113 |
| medical devices; immunology and microbiology devices; classification of respiratory viral panel multiplex nucleic acid assay. final rule. | the food and drug administration (fda) is announcing the classification of the respiratory viral panel multiplex nucleic acid assay into class ii (special controls). the special controls that will apply to the device are three guidance documents entitled: "class ii special controls guidance document: respiratory viral panel multiplex nucleic acid assay," as applicable, "class ii special controls guidance document: testing for human metapneumovirus (hmpv) using nucleic acid assays," and as applic ... | 2009 | 19877377 |
| viral entry mechanisms: the increasing diversity of paramyxovirus entry. | the paramyxovirus family contains established human pathogens such as the measles virus and human respiratory syncytial virus, as well as emerging pathogens including the hendra and nipah viruses and the recently identified human metapneumovirus. two major envelope glycoproteins, the attachment protein and the fusion protein, promote the processes of viral attachment and virus-cell membrane fusion required for entry. although common mechanisms of fusion protein proteolytic activation and the mec ... | 2009 | 19878307 |
| human metapneumovirus infection in hematopoietic stem cell transplant recipients. | human metapneumovirus (hmpv) was described in 2001 and has been associated with both upper and lower respiratory tract infection (urti and lrti, respectively), especially in children, the elderly, and in immunocompromised patients. the objective of this study was to identify hmpv as the etiological agent of acute respiratory infection in hematopoietic stem cell transplant (hsct) patients and to determine the clinical features of hmpv infection in these patients. | 2010 | 19878491 |
| comparison of clinical features of pediatric respiratory syncytial virus and human metapneumovirus infections. | | 2009 | 19888849 |
| [human metapneumovirus (hmpv) infections in young children]. | human metapneumovirus (hmpv) is a recently discovered respiratory virus. hmpv, the second after respiratory syncytial virus (rsv), is leading cause of upper and lower respiratory tract infections mainly in infants and children. hmpv infections have been reported in many countries in all the world, in patients in every age and gender, but children under 5 years are most likely to be susceptible to infections caused by hmpv. the frequency of hmpv infections require hospitalization in this group is ... | 2009 | 19899593 |
| study of human metapneumovirus-associated lower respiratory tract infections in egyptian adults. | there is a deficiency in the data concerning the role of hmpv in lower respiratory tract infections in adults, and until now there has been no data available regarding the prevalence of hmpv in adults in our region. in the present study the association of hmpv with varieties of lower respiratory tract disorders in immunocompetent adult patients, either alone or with bacterial pathogens, has been highlighted. eighty-eight patients were included in the study. they included 46 males and 42 females ... | 2009 | 19903260 |
| human metapneumovirus m2-2 protein inhibits viral transcription and replication. | m2-2 protein of human metapneumovirus (hmpv) is encoded by one of two overlapping open reading frames within m2 mrna. the precise function of hmpv m2-2 protein remains unknown. we here examined effect of m2-2 protein on hmpv transcription and replication using a minigenome construct and monitoring luciferase reporter gene expression. the minigenome assays demonstrated that m2-2 protein inhibited both transcription and rna replication. the inhibitory function of m2-2 protein was completely abroga ... | 2010 | 19913636 |
| development and evaluation of a whole virus-based enzyme-linked immunosorbent assay for the detection of human metapneumovirus antibodies in human sera. | to apply serological testing for human metapneumovirus (hmpv) to large-scale sera samples, an enzyme-linked immunosorbent assay (elisa) was developed in which purified virions were used as the antigen. the elisa was evaluated using 102 human sera specimens from patients aged 0-59 years. there was a positive association between the elisa results and neutralization test titers, with the correlation coefficients being greater in children <6 years old (rho=0.899, p<0.0001), which is consistent with ... | 2010 | 19925829 |
| [epidemiological and clinical description of human metapneumovirus infectious diseases in children]. | human metapneumovirus (hmpv), a recently identified respiratory virus, is a leading cause of acute respiratory tract infection in children during winter. the aims of this study were to outline epidemiological and clinical presentations of hmpv infectious diseases in young children. | 2010 | 19932012 |
| sole pathogen in acute bronchiolitis: is there a role for other organisms apart from respiratory syncytial virus? | acute bronchiolitis (ab) is a common disease of young children with peak incidence during the winter season. respiratory syncytial virus (rsv) is a major causative organism, yet recent relatively small sized studies have suggested an increased role of other organisms as sole or codetected organisms. the aim of this study was to assess the prevalence of sole- and mixed-organisms infections in hospitalized children with ab, using combined antigen-based and polymerase chain reaction assays (pcr). | 2010 | 19935450 |
| generation of recombinant nucleocapsid protein of human metapneumovirus in baculovirus for detecting antibodies in the beijing population. | human metapneumovirus (hmpv) is associated with respiratory infections in both adults and children. recombinant nucleocapsid (n) proteins of hmpv from two major groups of hmpv in beijing with a 6x his-tag at the c terminus were constructed in a baculovirus and expressed in transfected sf21 insect cells. the antigenicity and specificity of the expressed recombinant proteins were examined by immunofluorescence assay and western blotting. preliminary use of the expressed proteins for antibody detec ... | 2010 | 19937067 |
| viral pathogens including human metapneumovirus are the primary cause of febrile respiratory illness in hiv-infected adults receiving antiretroviral therapy. | to determine the spectrum of pathogens causing acute febrile respiratory illness in human immunodeficiency virus (hiv)-infected adults, we re-analyzed data from a prospective surveillance study involving 50 outpatients (90% of whom received highly active antiretroviral therapy). nasopharyngeal samples were tested for 23 respiratory viruses by multiplex reverse-transcriptase polymerase chain reaction (pcr) and for atypical bacteria by pcr. sputum cultures and serological testing were performed. v ... | 2010 | 19951142 |
| [prevalence and clinical features of human metapneumovirus infection in hospitalized pediatric patients with respiratory tract infection in suzhou area]. | to determine whether human metapneumovirus (hmpv) was circulating in suzhou area and the epidemiology and clinical features associated with hmpv infection. | 2009 | 19951497 |
| [not only rsv can cause bronchiolitis in small children. human metapneumovirus maybe the the second most common cause]. | | 2009 | 19998817 |
| humoral immunity to human metapneumovirus infection in adults. | human metapneumovirus (hmpv) is a significant cause of respiratory illness in children and adults. presently, there are no human data regarding the role of antibody for protection against hmpv illness. therefore, we measured serum and nasal antibody titers against hmpv by eia and neutralization assay at baseline in hmpv infected adults compared with subjects who remained uninfected. antibody titers were also compared in patients with mild and severe illness. mean serum binding and neutralizing a ... | 2010 | 20003919 |
| implementation of real-time rt-pcr for detection of human metapneumovirus and its comparison with enzyme immunoassay. | human metapneumovirus (hmpv) is responsible for outbreaks of bronchiolitis in winter and early spring in young children. due to the relatively recent discovery of hmpv, the diagnostic opportunities are limited, while differential diagnosis with respiratory syncytial virus (rsv) remains important. we validated the rt-pcr by comparing various methods of rna extraction, one-step rt-pcr kits and primer-probe combinations. the optimized rt-pcr was evaluated using 47 nasopharyngeal aspirates (npas) co ... | 2010 | 20013132 |
| metapneumovirus and its place in childhood. | human metapneumovirus (hmpv) was recently identified as a cause of acute upper and lower respiratory tract infection in children and adults. the epidemiology is similar to that exhibited by respiratory syncytial virus, with most episodes occurring during the winter months. the virus likely has a worldwide distribution. almost all children have been infected by five years of age. the suspicion of hmpv infection should be higher in infants or children presenting with symptoms compatible with a vir ... | 2003 | 20019852 |
| high seroprevalence of neutralizing capacity against human metapneumovirus in all age groups studied in bonn, germany. | human metapneumovirus (hmpv) infections occur frequently despite high rates of perpetual seroprevalence for all age groups. analyses of approximately 2,000 archived, randomly selected serum samples demonstrated that neutralizing capacities remain high, with a minor decrease for individuals over 69 years of age, leading to the hypothesis that reinfections occur because humoral immune responses play minor roles in the clearance of hmpv infections. | 2010 | 20042516 |