| [cloning of coxsackie virus b 3]. | | 1986 | 3008770 |
| rapid identification of coxsackie b viruses after immunoprecipitation and nucleic acid hybridization. | to circumvent the difficulties in concentrating sufficient virus from a clinical or environmental sample for detection and identification, we have used immunoprecipitation to rapidly concentrate coxsackie b viruses from both large and small sample volumes. antiviral serum and killed staphylococcus aureus cells as a protein a source were used to bind and collect the virus. radioactively-labeled viral nucleotide sequences were used to identify the collected virus by nucleic acid hybridization. the ... | 1986 | 3009085 |
| a comparison of the susceptibility of three human gut tumour-derived differentiated epithelial cell lines, primary monkey kidney cells and human rhabdomyosarcoma cell line to 66-prototype strains of human enteroviruses. | the growth of prototype strains of 31 serotypes of echo, 3 polio, 6 coxsackie b, 24 coxsackie a and enterovirus serotypes 70 and 71 were tested in parallel in primary monkey kidney cells (pmk), rd cells and three gut tumour-derived differentiated epithelial cell lines (hrt-18 ht-29 in skco-1). all 31 serotypes of echo viruses grew in ht-29, 27 and skco-1, 5 in hrt-18, 29 in pmk and 29 in rd. there was good growth of poliovirus serotypes in all five cell types. coxsackie b viruses grew well in al ... | 1985 | 3009511 |
| coxsackie b-2 virus infection in rat beating heart cell culture. | beating rat heart cultures were prepared in vitro and infected with coxsackie b-2 virus. the cells were evaluated in the post-infected period for changes in cardiac enzymes, alterations in beating frequency and cytotoxicity as measured by chromium 51 (51cr) release. the cardiac enzymes, lactate dehydrogenase (ldh) and aspartate aminotransferase (ast) were measured in infected and uninfected controls over a period of 120 h. enzyme levels in the infected cells remained essentially the same for the ... | 1985 | 3009512 |
| use of the nitrocellulose-enzyme immunosorbent assay for rapid, sensitive and quantitative detection of human enteroviruses. | a modified enzyme immunosorbent assay (eia) employing nitrocellulose (nc) membrane as a high-capacity solid phase was successfully employed for the sensitive and rapid detection of human enteric viruses, poliovirus and coxsackievirus b-5. the sensitivity of the nc-eia ranged from 7 to 70 pg of viral antigen diluted in phosphate-buffered saline. when virus was added to crude supernatants of mollusc tissue homogenates prepared by the standard procedure for the recovery of viruses in molluscs, the ... | 1985 | 3009513 |
| the role of the cd8-positive subset of t cells in proliferative responses to soluble antigens. ii. cd8-positive cells are not responsible for dr-associated differences in responsiveness to mumps and coxsackie b4. | the role of cd8 (t8, leu 2)-positive t lymphocytes in the proliferative t-lymphocyte response to mumps and coxsackie b4 viral antigens in vitro was investigated. the frequency among enriched t-lymphocyte blasts of antigen-reactive t lymphocytes (artl) restricted by different dr-associated elements was investigated, using antigenic restimulation with allogeneic antigen-presenting cells in a limiting dilution assay. a decreased frequency of dr3-restricted and an increased frequency of dr4-restrict ... | 1986 | 3010444 |
| an incidence peak of juvenile diabetes. relation to coxsackie b virus immune response. | all new cases of insulin dependent diabetes mellitus (iddm) in children below 15 years of age were recorded prospectively during a 21-year period 1964-1984 in a defined uptake area with a relatively constant child population. the total number of children recorded was 222-111 boys and 111 girls. the number of new cases varied between 4 cases in 1968 and 20 in 1984; in 1983 seventeen new cases were recorded. specific igm antibodies against coxsackie b virus (cbv), types 1-5 were measured by a reve ... | 1985 | 3010631 |
| optimization of the bgm cell line culture and viral assay procedures for monitoring viruses in the environment. | an in-depth study of the continuous cell line designated bgm is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, sera, nahco3 levels, culture bottles, cell concentration, overlay media, agar, virus infection conditions, and cell-dissociating agents. additionally, a comparative virus isolation ... | 1986 | 3010860 |
| coxsackie virus b4 produces transient diabetes in nonhuman primates. | cynomolgus, rhesus, and cebus monkeys failed to show glucose tolerance or insulin secretion abnormalities after infection with encephalomyocarditis virus or coxsackie virus b4. patas monkeys also showed no abnormalities after infection with encephalomyocarditis virus. however, patas monkeys infected with coxsackie virus b4 or treated first with a subdiabetogenic dose of streptozocin and then infected sequentially with coxsackie viruses b4 and b3 showed transient elevation of glucose tolerance te ... | 1986 | 3011574 |
| coxsackievirus b2 infection in a neonate with incontinentia pigmenti. | because of the concern for herpes simplex virus infection in the neonate, the presence of neonatal vesiculobullous lesions is a critical finding. however, there are other etiologies for these lesions. a case of a neonate with a vesicular rash and meningoencephalitis which was initially thought due to herpes is presented. the infant was ultimately determined to have incontinentia pigmenti and a concomitant coxsackievirus b2 infection. | 1986 | 3012453 |
| effects of viral infection on contraction of the diaphragm in mice. | isometric contractile properties of isolated phrenic nerve-diaphragm muscle preparations were used to study the effects of picornavirus infections on diaphragm muscle function. properties of muscles from virus-inoculated and control mice were similar during brief contractions. however, when subjected to a series of fatiguing contractions by indirect or direct stimulation, muscles of mice inoculated with a paralytic variant of encephalomyocarditis (emc) virus showed a greater rate of fatigue and ... | 1986 | 3012575 |
| genetic predisposition to diabetes mellitus is associated with impaired humoral immunity to coxsackievirus b4. | experiments were performed to determine whether genetic predisposition to diabetes mellitus (dm) or clinical dm or both exert an influence on the production of neutralization antibodies to coxsackievirus b4 (cb4). the homozygous diabetic mutant mouse db+/db+, on the inbred c57bl/ksj genetic background, develops a diabetes-like disease when maintained on ad libitum diet but restriction of excess food intake prevents overt disease. the doubly heterozygote db+/+m or the homozygote +m/+m misty coat ... | 1986 | 3013555 |
| coxsackie b virus-specific igm antibody and myocardial infarction. | the elisa technique was shown to be group-specific for the detection of igm antibodies against coxsackie b viruses, and probably against a wider range of enteroviruses. no evidence was obtained that recent coxsackie b-virus infection predisposes to myocardial infarction. | 1986 | 3014150 |
| evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses. | four continuous cell lines, bgm, l-132, hel-299, and rd, were compared both when cultured separately and as mixtures for use in plaque assay titrations of human adenovirus 1 and six human enterovirus serotypes. the effect of incubating these cell cultures in media containing 5-iodo-2'deoxyuridine (idu) prior to inoculation with virus was also studied. the use of mixed-cell cultures revealed cell line-dependent synergistic effects as well as inhibitory effects. these effects were strongly virus d ... | 1986 | 3015021 |
| recovery of viruses from water by a modified flocculation procedure for second-step concentration. | a reduction in virus recovery efficiencies stemming from a change in the commercial processing of powdered beef extract was reversed by the addition of celite analytical filter aid. supplementing beef extract with this silicate is recommended as a modification to the organic flocculation procedure for second-step concentration in monitoring for waterborne viruses. considerable differences in virus recovery were found among lots of beef extract and celite preparations; this indicates that the per ... | 1986 | 3015024 |
| coxsackie b4 virus induces short-term changes in the metabolic functions of mouse pancreatic islets in vitro. | mouse pancreatic islets cultured in vitro were infected with a tissue culture-adapted or a mouse pancreas-adapted strain of coxsackie b4 (cb4) virus. the effects of the viruses on the islets were assessed by examination of their biochemical functions. it was found that the mouse pancreas-adapted strain of cb4 induced a 'leakage' of insulin from islets incubated at a basal (2 mmol l-1) glucose concentration, both at two and four days following infection. however, at a stimulatory concentration of ... | 1986 | 3015449 |
| susceptibility of endothelial cells derived from different blood vessels to common viruses. | we examined whether endothelial cells derived from different blood vessels vary in their susceptibility to viral infection. five common viral pathogens of humans (herpes simplex 1, measles, mumps, echo 9, and coxsackie b4 viruses) were evaluated for growth in endothelial cells derived from bovine fetal pulmonary artery, thoracic aorta, and vena cava. all five viruses replicated in each type of endothelial cell. there were apparent differences in the quantities of measles and mumps viruses produc ... | 1986 | 3015862 |
| concurrent cytomegalovirus and coxsackie b virus infections in a heart-lung transplant recipient. | a 35-year-old woman with fibrosing alveolitis was given a combined heart and lung transplant at papworth hospital, cambridge. although her immediate post-operative course was satisfactory, she subsequently experienced concurrent primary cytomegalovirus and coxsackie b virus infections associated with significant morbidity including pancreatitis. she made a full recovery and has remained well for more than 2 years after transplantation. | 1986 | 3016100 |
| mu-antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis. | between august and november 1985, 45 patients with suspected aseptic meningitis were investigated using conventional virus isolation procedures and the mu-antibody capture coxsackie b igm enzyme-linked immunoabsorbent assay (elisa) test, which is well known to cross-react with other members of the enterovirus group. an enterovirus was isolated from 22% of patients compared with 67% who were positive in the elisa test. not only was the rate of enterovirus detection increased by using this elisa m ... | 1986 | 3016164 |
| humoral immune response in hereditary and overt diabetes mellitus. | the inbred diabetic mutant mouse, c57bl/ksj db +/db + (db +/db +), spontaneously develops diabetes mellitus when allowed food ad libitum. however, restriction of food intake prevents the expression of this genetic predisposition for diabetes. this experimental design has been used previously to demonstrate a deficient neutralizing antibody response to coxsackievirus b4 (cb4) in mutants with the genetic predisposition only. these observations demonstrate that in the genetically predisposed diabet ... | 1986 | 3016167 |
| demonstration of suppressor cells in coxsackievirus group b, type 3 infected female balb/c mice which prevent myocarditis. | coxsackievirus group b type 3 (cvb3) induces myocarditis in male balb/c mice but produces little cardiac injury in females. males develop cytolytic t lymphocytes (ctl) reactive to heart antigens which primarily cause the inflammation and cardiac injury observed in the disease. infected female mice lack this ctl response because they rapidly produce suppressor cells inhibiting both cellular immunity and cardiac inflammation. four lines of evidence demonstrate suppressor cells in females. first, f ... | 1986 | 3017580 |
| involvement of natural killer cells in coxsackievirus b3-induced murine myocarditis. | the role of natural killer cells in the temporal development of coxsackievirus b3-induced myocarditis in adolescent cd-1 male mice was examined. inoculation of purified cvb3m induced maximum nk cell activity in the splenic populations at 3 days postinoculation (p.i.) as assessed by lysis of yac-1 cells; maximum virus titers in heart tissues were also found at day 3 p.i. mice depleted of nk cells after injection of anti-asialo gm1 antiserum i.v. had decreased nk cell activity, increased cvb3m tit ... | 1986 | 3018079 |
| protein kinase in nondiabetogenic coxsackievirus b4. | alkali-dissociated, purified preparations of prototype coxsackievirus b4 release a protein kinase that catalyzes the incorporation of gamma-phosphate from 32p-labeled atp into three virus capsid proteins (vp1, vp3, vp4), several additional proteins of the particle, and exogenous acceptor proteins. using protamine sulfate as an acceptor protein, we detected nearly 20-fold more enzyme activity in membrane-bound virions (mbv) than in virions of the virus. the activity in the mbv is cyclic nucleotid ... | 1986 | 3018140 |
| coxsackievirus b3 infection alters plasma membrane of neonatal skin fibroblasts. | replication of coxsackievirus b3 occurred for days in cultures of murine neonatal skin fibroblasts in the absence of cytopathology and resulted in alteration of the plasma membrane. dual immunofluorescence studies showed that the lectin ulex europaeus agglutinin i bound only to cells producing viral capsid antigens. cultures of coxsackievirus b3-inoculated murine neonatal skin fibroblasts showed maximum binding of this lectin at 72 h postinoculation. these data show that in a nonlytic infection ... | 1986 | 3018294 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | | 1986 | 3018334 |
| [experimental models of insulin-dependent diabetes]. | | 1986 | 3020804 |
| mechanism of inactivation of enteric viruses in fresh water. | fresh water obtained from nine sources was shown to cause inactivation of poliovirus. further testing with four of these water samples showed that enteric viruses from different genera were consistently inactivated in these freshwater samples. studies on the cause of inactivation were conducted with echovirus type 12 as the model virus. the results revealed that the virucidal agents in the waters tested could not be separated from microorganisms. any treatment that removed or inactivated microor ... | 1986 | 3021056 |
| enumeration of enterovirus particles by scanning electron microscopy. | enumeration of virus particles requires relatively concentrated and uniformly dispersed virus preparations, which is difficult to achieve by the usual methods of negative staining and transmission electron microscopy. we have developed an electrophoretic method that concentrates enteroviruses onto a polycarbonate membrane for examination by high-resolution scanning electron microscopy. the electrophoretic apparatus comprises three chambers in electrical series, each containing 3.5 ml of dilute b ... | 1986 | 3021798 |
| coxsackievirus group b replication in cultured fetal baboon aortic smooth muscle cells. | all six coxsackievirus b (cvb) serotypes replicated to various extents in fetal baboon aortic smooth muscle cells (smc) in culture. cvb3 and cvb4 replicated to the highest titers and induced no cytopathology at the level of light microscopy. maximum yields of cvb3 were produced between 12 and 24 hr postinoculation. up to 15% of smc cells became infected, as determined by immunofluorescence assays with anti-cvb3 antiserum, yet overall cell division in infected cultures did not differ from infecte ... | 1986 | 3021897 |
| [late sequelae after coxsackie virus b myocarditis]. | | 1986 | 3022417 |
| liquefactive necrosis in coxsackie b encephalitis. | coxsackieviruses may cause serious illness in infants and children, specifically myocarditis and meningoencephalitis. central nervous system lesions have been characterized as inflammatory in nature with mononuclear cell infiltration, neuronophagia, and glial nodule formation largely confined to the brain stem and spinal cord. we present two infants with documented coxsackie b virus infection who also had widespread multifocal areas of liquefaction necrosis unassociated with inflammation. such a ... | 1986 | 3022671 |
| [the pathological observation of experimental infections by coxsackie virus b4 (cvb4) in mice]. | | 1986 | 3022952 |
| coxsackievirus b-3 myocarditis. t-cell autoimmunity to heart antigens is resistant to cyclosporin-a treatment. | a cardiotropic variant of coxsackievirus group b, type 3 (cvb3) induces myocarditis in inbred balb/c mice. myocardial injury is predominantly mediated by t lymphocytes recognizing normal myocyte antigens, making this an autoimmune disease. nonetheless, the autoimmune response cannot be inhibited by cyclosporin a (csa) treatment of the infected animals. mortality in treated mice was increased 2-4 times, but neither virus-specific antibody or cytolytic t-lymphocyte responses were affected, and max ... | 1986 | 3024491 |
| myocarditis in mice infected with coxsackie virus b3. | perimyocarditis in the heart of balb/c mice infected with coxsackie virus group b type 3 (cb3) was studied to determine whether it is limited to the right perimyocardium and to show whether or not perimyocarditis or myocardial lesions are produced in both left and right ventricles. cb3 was recovered from the heart on days from 2 to 13 after inoculation, but thereafter no virus was isolated from any part of the heart. histopathologically, from days 1 to 4, hyaline or granular degeneration and nec ... | 1986 | 3024693 |
| a microneutralization test for the identification of enterovirus isolates. | two-hundred and thirty-four enterovirus isolates were identified using a recently modified, microneutralization procedure. neutralization tests were performed in 96 well plastic 'v' plates, using 20 microliter quantities of antisera and virus, then inoculated onto monolayers of buffalo green monkey kidney cells, growing in 48 well tissue culture plates. utilization of this microneutralization procedure resulted in considerable savings of time, material, and particularly, neutralizing antisera. | 1986 | 3025242 |
| [pathogenesis of atrial myocarditis in monkeys inoculated with cb1oxsackie virus b3]. | | 1986 | 3025310 |
| [neonatal myocardial infarct associated with maternal-fetal infection due to coxsackie b4 virus]. | heart failure with myocardial infarction occurred in the course of a neonatal infection with aseptic meningitis. coxsackie b4 infection was found in the child and its mother. the unusual myocardial action of the virus, coronary embolus, intermediate role of a pancreatitis. by age 8 months, under digitalis glycosides treatment, growth and psychomotor development were normal. | 1986 | 3026276 |
| adult onset still's disease or coxsackie polyarthritis? | the clinical manifestations and laboratory findings of two patients with a presumptive diagnosis of coxsackie b4 virus infection are described. a striking feature was the similarity with adult onset still's disease, with spiking fever, evanescent macular rash, and severe polyarthritis. this latter feature persisted for many weeks and required steroids to control the symptoms. review of the literature has supported the proposition that many cases of adult onset still's disease may be due to coxsa ... | 1986 | 3026295 |
| acquired immunity and allergy of cells cultured in vitro. | it is in the reinoculated cell line fl that the sensitivity of cells under the action of a small dose of diphtheria toxin (dt) changes to the toxin itself as well as to the coxsackie enterovirus. (b-5). after surviving the infection and getting again "healthy", the cells have acquired simultaneously an elevated sensibility to dt and reduced susceptibility to b-5 transmissible even in long passages. these acquired properties are a transient phenomenon, after a certain number of passages the cultu ... | 1986 | 3027165 |
| [experimental diabetes in mice infected with coxsackie viruses]. | the influence of coxsackie b4 and ai3 viruses on the pancreas of mice (resistant and susceptible to diabetes) was studied. glucose intolerance and changes in the synthesis of immunoreactive insulin were detected in all the treated groups of animals. biochemical changes were more prominent in male dba/2 mice, infected with coxsackie b4 virus, in fi (cba x c57bl/6) hybrids and in female dba/2 mice infected with coxsackie ai3 virus and alloxan. | 1987 | 3028528 |
| royal free disease: perplexity continues. | | 1987 | 3028544 |
| elimination of human enteric viruses during conventional waste water treatment by activated sludge. | the present study was undertaken to determine if viruses were selectively eliminated during waste water treatment. human enteric viruses were detected at all steps of treatment in a conventional activated sludge waste water treatment plant. liquid overlays and large volume sampling with multiple passages on bgm cells permitted the detection of poliovirus (serotypes 1, 2, and 3), coxsackievirus b (serotypes 1, 2, 3, 4, and 5), and echovirus (serotypes 3, 14, and 22), as well as reoviruses. the me ... | 1986 | 3028589 |
| selection of coxsackievirus b4 variants with monoclonal antibodies results in attenuation. | inoculation of suckling mice with coxsackievirus b4 (cb4) results in the death of a majority of the animals. in this study we selected antigenic variants of cb4 in the presence of neutralizing monoclonal antibodies and tested them to see whether they were attenuated. antigenic variants selected with a single antibody showed little or no attenuation by producing a high mortality (60 to 100%). a double variant selected with two antibodies showed considerable attenuation by causing only 25% mortali ... | 1987 | 3029310 |
| immunohistochemical study of the myocardium in murine coxsackie b3 virus myocarditis using monoclonal antibodies--significance of lyt 1 antigen-bearing lymphocytes in cell-mediated immunity. | this light- and electron-microscopic study using monoclonal antibodies provides an immunohistochemical demonstration of lymphocytes in situ in the myocardium in murine coxsackie b3 virus myocarditis. on the 7th and 9th days after virus inoculation, we observed many necrotic cardiocyte foci infiltrated with numerous inflammatory cells including macrophages and t lymphocytes. there were only a few lyt 1-bearing lymphocytes in this phase of inoculation. on the 14th day there were many lyt 1-bearing ... | 1986 | 3029449 |
| myocarditis with myocardial infarction like findings in a 3-year old girl. | a 3 year-old japanese girl had an acute onset associated with vomiting. the electrocardiogram (ecg) indicated changes similar to those of acute myocardial infarction (mi); there was no past history of kawasaki disease. selective coronary angiography taken on the 28th day of illness revealed no abnormality. thallium 201 scintigraphy was also performed and it revealed that the area of absent myocardial uptake was in the anterior wall. in serological findings, antibody titers against coxsackie b-3 ... | 1986 | 3029450 |
| coxsackievirus b-3-induced myocarditis. effect of sex steroids on viremia and infectivity of cardiocytes. | male and female balb/c mice were inoculated with various concentrations of coxsackievirus, group b, type 3 (cvb3), ranging from 10 to 10(7) plaque-forming units (pfu). lower viral doses (greater than 10(2) pfu) induced severe myocarditis in male mice but caused little injury in females. with 10(7) pfu, females also developed severe disease. females may be relatively resistant to cvb3-induced myocarditis because virus entry into the blood and heart is less effective. males given 125i-cvb3 show ap ... | 1987 | 3030117 |
| heart-specific autoantibodies induced by coxsackievirus b3: identification of heart autoantigens. | postinfection sera from a.ca/snj a.sw/snj, b10.s/sgsf, and b10.pl/sgsf mouse strains which varied in their susceptibility to coxsackievirus b3-induced immunopathology were suspected to contain autoantibodies against cardiac tissue. these sera were used to identify the target myocardial autoantigen(s). sera pools were made during the peak of the early, virus-induced myocarditis at 5 and 7 days and during the peak of the late, immunopathic phase of myocarditis at days 15 and 21 after infection. on ... | 1987 | 3030591 |
| [the hla system and coxsackie b viral myocarditis in adults]. | the distribution of hla antigens a, b and c was studied in 152 normal donors, 53 patients with coxsackie b virus myocarditis, 35 patients with myocarditis of unknown origin, 16 coronary patients and 14 rheumatic patients with high titres of anti-coxsackie virus antibodies. coxsackie virus myocarditis was associated with increased occurrence of hla antigens a3, b40, cw2 and a28, the increase being significant (with an adjustment to the number of the tested antigens) for a3 in severe and medium my ... | 1986 | 3031353 |
| cold reacting antilymphocyte antibodies in type i (insulin dependent) diabetes. | non hla antilymphocyte antibodies have been detected in numerous auto-immune illnesses and notably in type i (insulin dependent) diabetes. in order to ascertain their role in this illness, the actual frequency of this phenomenon has been estimated. the privileged cellular target has been determined. finally correlations with the other immunogenetic markers of diabetes have been investigated. non hla antilymphocyte antibodies are frequent, above all at the beginning of the illness (32.4% before 6 ... | 1987 | 3032702 |
| coxsackievirus b 1 induced murine polymyositis: acute infection with active virus is required for myositis. | although the etiology of human polymyositis (pm) remains obscure, group b coxsackieviruses (cvb) have been implicated in disease pathogenesis and a particular strain of type 1 cvb (cvb 1) has been shown to cause in mice an inflammatory myositis which is similar to human pm. after infection of neonatal swiss mice with active cvb 1, virus replicated to high titers in muscle and produced acute myonecrosis. viral titers peaked in muscle at day 7 and virus was undetectable after day 14. myocytes rege ... | 1987 | 3033235 |
| [intrahospital outbreak of enterovirus encephalitis]. | the authors examined 9 children aged from 13 months to 9 years who, while being in a general hospital, contracted enteroviral encephalitis induced by coxsackie b3 virus with a possible involvement of coxsackie b1 virus. this report on a group hospital outbreak of enteroviral encephalitis appears to be the first of the kind in the available literature. the authors discuss the possibility of enteroviral encephalitis and economo's encephalitis being identical in nature. | 1987 | 3033954 |
| coxsackievirus b3-induced myocarditis. autoimmunity is l3t4+ t helper cell and il-2 independent in balb/c mice. | male balb/c mice inoculated with 6 x 10(4) plaque-forming units (pfu) coxsackievirus, group b, type 3 (cvb3), develop myocarditis within 7 days. two cytolytic t lymphocyte (ctl) populations arise in infected animals. one population belongs to the lyt 2+ t (cytolytic/suppressor) lymphocyte subset and reacts specifically with uninfected heart cells (autoreactive ctls, actls), whereas the other belongs to the l3t4+ t (helper) lymphocyte subset and reacts with infected targets (virus-specific ctls, ... | 1987 | 3034068 |
| synergistic antiviral effect of xanthates and ionic detergents. | xanthate compounds have been shown to exhibit antiviral activity against various dna and rna viruses under acidic ph conditions. it is now possible to utilize the unique broad range antiviral spectrum of these compounds under physiological ph conditions (ph 7.4) by simultaneous administration of certain ionic detergents. when used in conjunction with tricyclodecan-9-yl-xanthate (d609), sodium deoxycholate, sodium dodecylsulfate and certain fatty acids, which have no antiviral activity of their o ... | 1987 | 3034292 |
| cytotoxic islet cell autoantibodies in newly diagnosed insulin-dependent diabetes mellitus: lack of correlation to age, residual beta cell function, hla antigens and coxsackie b virus antibodies. | in a cross-sectional study comprising of 56 patients with newly diagnosed insulin-dependent diabetes mellitus (iddm) serum was examined for the presence of complement-dependent antibody mediated cytotoxicity (c'amc) by an improved assay measuring the release of 51cr from freshly isolated normal rat islet cells prelabeled with the isotope. in the presence of complement, 35 (63%) iddm sera specifically mediated cytotoxicity against islet cells. the degree of cell specificity was tested using prela ... | 1987 | 3034475 |
| regional assignment of five genes on human chromosome 19. | a human-mouse hybrid segregant hm76dd40-6 with new characteristics was derived from the hybrid cell line hm76dd containing human chromosome 19 as the only human chromosome. three virus sensitivities located on human chromosome 19 (pvs, e11s and rdrc) were lost in hm76dd40-6, while six other genes (c3, ldlr, ef2, gpi, pepd and manb) were retained. cytogenetic analysis and in situ hybridization using human or mouse repeated sequences as probes showed that the region q13.1-qter of human chromosome ... | 1987 | 3034518 |
| [results of virologic studies in 620 children with abacterial meningitis over a 15-year period (1971-1985)]. | cases of non-bacterial meningitis being hospitalized at the children's hospital of the university of bochum in the years 1971 to 1985 were analysed retrospectively according to etiological and epidemiological aspects. altogether 620 non-bacterial cases of meningitis were included in this study. for 378 (60.9%) children the infecting agent could be identified while in 242 cases (39.1%) the etiology remained open. in 237 cases enterovirus had been the causative agent. the average age of the patien ... | 1987 | 3035366 |
| [coxsackie b virus infections in cardiology. apropos of 66 cases]. | coxsackie b virus infections are common and frequently asymptomatic. however in young people, they can cause primary congestive cardiomyopathy and complicate previous cardiovascular illnesses. virus diagnosis is difficult and based mainly on the detection of significant rising or stating high neutralizing antibody titers. a clinical and epidemiological five years study investigated 3,856 sera. 30.2% of the patients had evidence of a significant antibody titer (greater than or equal to 64) to one ... | 1987 | 3035466 |
| [clinical picture of coxsackie b virus myocarditis in children]. | | 1986 | 3035473 |
| murine natural killer cells limit coxsackievirus b3 replication. | previous indirect evidence suggested that natural killer (nk) cells play a role in coxsackie virus b3 serotype 3, myocarditic variant (cvb3m)-induced myocarditis by limiting virus replication. in this study, we present direct evidence that nk cells can limit cvb3m replication both in vitro and in vivo. virus titers are lowered in primary murine neonatal skin fibroblast (mnsf) cultures incubated with activated splenic large granular lymphocytes (lgl) taken from mice 3 days postinoculation of cvb3 ... | 1987 | 3036947 |
| perinatal transmission of coxsackievirus b3 in mice. | oral infection of pregnant mice with coxsackievirus b3 (cb3) late in gestation produced maternal viremia, which peaked three to four days after challenge, then rapidly diminished. ninety percent of mice developed igg antibody to cb3 by ten days after challenge. cb3 titers from placental tissue peaked at two to four days after maternal infection, but moderate titers persisted for at least eight days. though virus could be recovered (less than 1.0-1.5 log10 pfu/g) from fetal tissue in only a small ... | 1987 | 3036959 |
| reemergence of an epidemic coxsackievirus b5 genotype. | outbreaks of coxsackievirus b5 (cb5) infections occur primarily during peak epidemic years, with comparatively few cases occurring during intervening years. this pattern of periodic cb5 epidemicity is quite distinct from the general endemicity typical of other group b coxsackieviruses. to determine the genetic relationships among cb5 isolates from different outbreaks, we compared viral rnas by ribonuclease t1 oligonucleotide fingerprinting. isolates obtained within an epidemic year had very simi ... | 1987 | 3036966 |
| the complete nucleotide sequence of coxsackievirus b4 and its comparison to other members of the picornaviridae. | the genome of the prototype stain of coxsackievirus b4 (j.v.b. benschoten) has been cloned in escherichia coli and its complete nucleotide sequence determined. excluding the poly(a) tract, the rna genome is 7395 nucleotides in length and appears to encode a single polyprotein of 2183 amino acids. the predicted amino acid sequence of the polyprotein shows close homology (88%) to that of the previously sequenced coxsackievirus b3 and to certain regions of the polyproteins of the polioviruses and h ... | 1987 | 3037008 |
| [persistent infection caused by the coxsackie b3 virus in adult mice]. | a model of persistent infection with coxsackie b-3 virus was developed in adult mice with clinical manifestations of the disease and long-term (up to 13 months) excretion of the causative agent. the method of multiple organ cultures was shown to be suitable for isolation of the persisting enterovirus. the presence of persistent infection was confirmed by the detection of igm antibody in repeated daily examinations of the animals for 4 months. it seems to be expedient to use this model for invest ... | 1987 | 3037797 |
| alteration of immune response to coxsackie b3 virus by streptozotocin in dual-aetiology diabetes mellitus in mouse. | | 1987 | 3038739 |
| myocardial lesions by coxsackie virus b3 and cytomegalovirus infection in infants. | immunofluorescent and electron-microscopic studies were performed to determine the distribution of viral antigens and particles and to clarify the relationship to myocardial lesions in two autopsy cases with generalized infection of coxsackie virus b3 (cvb3) or cytomegalovirus (cmv). case 1 was a full-term newborn female infant, without any congenital anomalies, who died of cardiac failure 10 days after birth. cvb3 was isolated from the blood before death. necrosis of the muscle fibers was obser ... | 1985 | 3038831 |
| cell-mediated immunity in coxsackie b3 virus myocarditis in mice--in situ characterization by monoclonal antibody of mononuclear cell infiltrates. | this light- and electron-microscopic study using monoclonal antibody and anti-immunoglobulin antibodies in murine coxsackie b3 virus myocarditis provides an immunohistochemical demonstration of surface antigens of lymphocytes. on the 7th and 9th days after inoculation, many necrotic cardiocytes were surrounded by numerous cellular infiltrates, in which macrophages and t lymphocytes predominated, whereas immunoglobulin-bearing b lymphocytes represented a minority. immuno-electron microscopy showe ... | 1985 | 3038832 |
| coxsackievirus b 1-induced polymyositis. lack of disease expression in nu/nu mice. | chronic inflammatory myositis similar to human polymyositis occurs in mice after infection with a strain of coxsackievirus b 1 (cvb 1). to investigate the role of t cells in the pathogenesis of this disorder, we compared disease expression in t cell-deficient athymic nude (nu/nu) mice and heterozygotes (nu/+) with normal t cell function. acute infectious myositis occurred in nu/nu and nu/+ mice. chronic (greater than 21 d postinfection) weakness and myositis, however, developed only in nu/+. res ... | 1987 | 3038960 |
| localized outbreak of enteroviral meningitis in adults. | during a thorough surveillance of viral infections of the central nervous system an outbreak of aseptic meningitis was discovered in the western part of finland in late 1985. the 21 diseased young adults were carefully studied by different virological methods. a presumed viral etiology, in all cases of enteroviral origin, was found in 16 of 20 (80%) with adequate specimens. four different enteroviruses were associated with this episode; in 9 cases the presumed etiological agent was echovirus 5, ... | 1987 | 3039780 |
| subclass restriction of human enterovirus antibodies. | we studied antibodies to enteroviruses in four groups of serum specimens: those from healthy adults, cord blood specimens, serum specimens known to contain immunoglobulin m (igm) to coxsackie b (cb) viruses by radioimmunoassay, and serum specimens from children with symptomatic enteroviral infections. enzyme-linked immunosorbent assays (elisas) were developed to detect the igg class- and subclass (igg1, igg2, igg3, and igg4)-specific responses to cb3. the cb3 virus elisa was not type specific. t ... | 1987 | 3040799 |
| biochemistry and pathogenicity of echovirus 9. iii. thermosensitive mutants of echovirus 9, strain barty, with reduced pathogenicity for newborn mice. | different clinical isolates of echovirus 9 are known to vary strikingly with regard to pathogenicity. prototype strain hill and strain barty have previously been shown to differ not only in paralytogenic potency for newborn mice but also in a number of in vitro characteristics related to virus capsid structures. a series of mutants of strain barty, thermosensitive for replication at 40 degrees c, was isolated after mutagenization with 5-fluorouracil. for all mutants the virus dose required to pa ... | 1988 | 3362095 |
| fatal echovirus type 7 in a premature infant. | echovirus 7 infections have not been previously recognized as fatal in neonates. a premature male member of a twin gestation developed an echovirus 7 infection at 5 days of age and succumbed to it. the clinical manifestations were similar to an echovirus 11 infection described in neonates. the second twin was free of illness as well as of virus shedding. | 1988 | 3382482 |
| morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9. | the membrane potential, em, of human granulocytes (pmns), was recorded using glass microelectrodes. the membrane potential em exhibited potential fluctuations accompanied by characteristic changes of cell shape. the periodic potential fluctuations (7-s, 70-s, and 260-s periodicities) ascertained by the autocorrelation technique, suggested the existence of an internal clock. the chemoattractant f-met-leu-phe (fmlp) had no influence on the periodicities, whereas the amplitudes of the fluctuations ... | 1988 | 3398429 |
| biochemistry and pathogenicity of echovirus 9. ii. mutants of echovirus 9, strain hill, with altered capsid surface properties. | the two echovirus 9 strains hill and barty have been shown previously not only to differ in pathogenicity for newborn mice but also in a number of in vitro characteristics which depend on viral capsid structure. three spontaneously occurring mutants of the mouse-apathogenic echovirus 9 prototype strain hill being resistant to an inhibitor of plaque formation present in agar were isolated and compared biochemically and biophysically to their parent strain and to the mouse-pathogenic echovirus 9 s ... | 1986 | 3739228 |
| isolation of echo virus type-22 from a child with acute myopericarditis--a case report. | | 1986 | 3744414 |
| [does virus diagnosis open new ways for the classification and treatment of sudden deafness, unilateral vestibular loss and idiopathic facial paralysis?]. | paired sera from 51 patients with sudden deafness, 21 patients with acute vestibular loss and 28 patients with bell's palsy were examined for antibodies against mumps-, echo-, herpes simplex- and coxsackie viruses. only two serum samples showed a significant titer increase or decrease. so viral etiology of sudden deafness, acute vestibular loss and bell's palsy seems to be unlikely and does not change the assignment and therapy of this disease. | 1986 | 3771296 |
| in vitro and in vivo anti-picornavirus activity of some p-benzoylphenoxypyridines. | fifteen p-benzoylphenoxypyridines were initially evaluated for their in vitro activity against rhinoviruses (rv) 1a, 2 and 64 and coxsackie virus (cox) a21 and for their oral prophylactic and therapeutic activity in swiss albino mice lethally challenged with cox a21. one compound, (4-[(5-methylsulfonyl-2-pyridinyl)oxy]phenyl) phenyl methanone, was selected for additional evaluation. these studies showed the compound to possess mic50 values of less than or equal to 5 micrograms/ml against only 6 ... | 1986 | 3777916 |
| slow virus diseases of the central nervous system. | | 1985 | 3893956 |
| risk factors associated with echovirus 11' infection in a hospital nursery. | an outbreak of nosocomial echovirus 11' illness occurred between july 22 and august 12, 1983, infecting 14 infants in the nurseries at a hospital. stool, throat or cerebrospinal fluid specimens for viral isolation were obtained from 142 and serum pairs from 98 of the 192 infants exposed to the nurseries during the outbreak. infection was determined by isolation of virus from stool or cerebrospinal fluid and/or a 4-fold rise in neutralizing antibody to echovirus 11'. eight infants were severely i ... | 1986 | 3952008 |
| persistence of echovirus 6 in cloned human cells. | establishment of a persistent infection by echovirus 6 in cloned human wish cells (pi) was demonstrated. the cloned human wish cells were maintained for 3.0 years (over 125 passages) and released virus continuously without cellular destruction. neither temperature-sensitive virus mutants nor interferon appears to play a role in either establishment or maintenance of viral persistence. the majority of the virus produced by cloned human wish cells is defective (2 x 10(6) particles per pfu) and dif ... | 1985 | 3973978 |
| [echovirus 11 outbreak among premature newborn infants in a neonatal intensive care unit]. | during a period of 3 weeks, 17 premature neonates 4 of them weighing less than 1000 g at birth became infected with echovirus 11 in a neonatal intensive-care unit. beside 3 inapparent infections, severe 'septicaemic' illness was observed among 5 premature neonates combined with meningitis and apnoeic attacks. 4 neonates presented meningitis as the predominant clinical feature and 3 showed gastrointestinal symptoms without neurological involvement. 2 children experienced a febrile illness with ap ... | 1985 | 4013560 |
| synthesis of antibodies, including antiviral antibodies, in the knee joints of patients with arthritis. | serum and synovial fluids from 16 patients with seronegative arthritis and eight with rheumatoid arthritis were studied for immunoglobulin levels and for antibody levels to five viruses. when allowances were made for the distribution of immunoglobulins between serum and synovial fluid there was evidence that in several patients antibody to one or more viruses was synthesised locally in the joint. igg and especially igm were present in greatly increased amounts in arthritic joints compared with n ... | 1985 | 4062387 |
| nuclear magnetic resonance probes for investigations of the virus-cell interactions. | measuring the nmr relaxation parameters of selectively excited protons within molecules bound to the cell membrane was shown to provide a profitable way of looking at the virus-cell interaction. such approach made it possible to delineate the virus adsorption to human red blood cells by using the nmr features of the cytoskeleton inhibitor colchicine. | 1985 | 4094564 |
| separation of echo virus type 6 antigens by centrifugation in caesium chloride density gradients. ii. electron microscopy. | | 1971 | 4103491 |
| virus meningitis in teesside. | | 1971 | 4103977 |
| a possible new enterovirus serotype isolated in ontario. | | 1971 | 4109494 |
| ultrastructural study of viral replication in human fetal intestinal organ culture. | | 1973 | 4121665 |
| coupling of peroxidase to poliovirus antibody: characteristics of the conjugates and their use in virus detection. | antibody to poliovirus type 1 (po-1) was coupled to peroxidase by use of glutaraldehyde or 4, 4' -difluoro,3, 3' -dinitro diphenyl sulfone. glutaraldehyde was found to be the superior coupling agent, yielding conjugates that had up to 2.8 x 10(4) enzyme units/ml (75% of total enzyme input). conjugates migrated as a single band when centrifuged in sucrose density gradients, demonstrating that the purification procedure used was effective in removing both noncoupled enzyme and heterogeneous antibo ... | 1973 | 4126429 |
| letter: virus particles in gastroenteritis. | | 1973 | 4129337 |
| progress with antiviral agents. | | 1974 | 4137135 |
| [virus-inactivating effectiveness of different methods of effluent treatment]. | | 1974 | 4154617 |
| protective action of benzimidazole derivatives against virus infections in tissue culture and in vivo. | | 1969 | 4179506 |
| [antiviral, antibacterial and antitoxic activity of normal human gamma globulins prepared at the hematology center of bucharest]. | | 1969 | 4186494 |
| establishment of a cynomolgus monkey kidney cell line and its susceptibility to various viruses. | | 1969 | 4186661 |
| studies of echovirus type 6 antigens in immunodiffusion. 1. antigen composition of native and heated virus preparations. | | 1968 | 4190285 |
| separation of echo virus type 6 antigens by centrifugation in caesium chloride density gradients. i. infectivity and antigenic activities. | | 1969 | 4192470 |
| interaction between sv 40 and other viruses in tissue culture: interference and double infection. | | 1969 | 4192471 |
| [viral antibody content in gamma globulin]. | | 1970 | 4193235 |
| echovirus type 17 in the neonate. | an outbreak of echovirus type 17 in a neonatal nursery with recovery of virus from the stools of the seven affected infants and the cerebrospinal fluid of one is described. intensive nursing care and supportive therapy prevented a possible fatal outcome in all instances. a further case in another community is described in which a 5-week-old child succumbed to his illness and echovirus type 17 was isolated from autopsy tissues - lung, liver, kidney and spleen. this child had presumably received p ... | 1973 | 4196425 |
| isolation of glucosamine from the capsids of a picornavirus. | | 1973 | 4200694 |
| isolation of a specific enhancer for echovirus 6 from uninfected permissive host cells. | a specific enhancer for the m+ variant of echovirus 6 was isolated from uninfected, permissive host cells. the enhancer transferred the susceptibility to virus infection from permissive cells to less susceptible cells. enhancer activity in cultured, human cells (wish) was released by these cells into extracellular fluids at a linear rate. maximal enhancer activity was recovered from cell monolayers that were extracted with buffered salt solutions (ph 6.6) for 5 h at 37 c. crude enhancer preparat ... | 1974 | 4202885 |