| the epididymis of the prepubertal swamp buffalo (bubalus bubalis): histochemistry of phosphatases. | the distribution of phosphatases was examined in the epididymis of swamp buffaloes aged 1.5-2 years, where spermatogenic activity in the testis had reached the early primary spermatocyte stage. a granular distribution of acid phosphatase was present in the luminal region of the epithelium of the efferent ductules. in the ductus epididymidis, four zones were identified and all zones showed varying degrees of acid-phosphatase activity in the epithelium, with the most pronounced activity in the api ... | 1996 | 8976610 |
| age-dependent variations of lactate dehydrogenase and creatine kinase activities in water buffalo calf serum. | the electrophoretic patterns of the serum enzymes lactate dehydrogenase and creatine kinase from water buffalo calves are described. differences in total activities as well as their relative distribution were seen at ages ranging from 1 to 10 weeks. while total lactate dehydrogenase activity increased by over 100%, total creatine kinase increased by almost 400%. the relative activities of lactate dehydrogenase 1 and 5 decreased with age. lactate dehydrogenase 2 and 3 increased and lactate dehydr ... | 1996 | 8986400 |
| high-resolution fish mapping of beta-defensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in ffttle standard karyotypes. | high-resolution chromosomal fluorescence in situ hybridization (fish) and r-banding allowed the chromosomal localization of the beta-defensin gene cluster (defb@) to chromosome band 1p12 in the river buffalo (bubalus bubalis, 2n = 50) and to band 24q13 in sheep (ovis aries, 2n = 54), according to standard nomenclatures. the localizations permitted the tentative assignment of the entire bovine syntenic group u25 to these chromosomes. a fish mapping control on cattle chromosomes confirmed the loca ... | 1996 | 8995479 |
| detection of species-specific genetic markers in farm animals through random amplified polymorphic dna (rapd). | the potential use of random amplified polymorphic dna (rapd) was evaluated as a source of development of alternative genetic markers for studying variation in buffalo (bubalus bubalis) and other related species of the artiodactyla family bovidae, in order to ascertain genetic relationships and diversities. fourteen arbitrary primers were used to amplify dna fragments in four species such as indian zebu cattle (bos indicus), buffalo (bubalus bubalis), sheep (ovis aries) and goat (capra hircus). c ... | 1996 | 9021403 |
| prevalence of infectious bovine rhinotracheitis in southern india. | the authors describe a serological survey on the prevalence of infectious bovine rhinotracheitis (ibr) among cattle and buffalo (bubalus bubalis) in three southern states of india. a local isolate of bovine herpesvirus 1 (bhv-1) from an outbreak of the respiratory form of ibr was used as a source of virus antigen in avidin-biotin enzyme-linked immunosorbent assay (elisa). the overall prevalence of antibodies to bhv-1 in cattle was 50.9%, and in buffalo was 52.5%. among breeding bulls, 114/120 sa ... | 1996 | 9025147 |
| vector abundance and behaviour in an area of low malaria endemicity in bataan, the philippines. | the vectorial importance of known and potential vectors in morong, bataan, philippines was assessed based on human and animal baited collections of adult mosquitoes and on larval collections. anopheles flavirostris, the principal vector in the philippines, was the most abundant among human landing catches, followed by an. maculatus sensu lato (s.l.). both showed similar seasonal abundance with a peak during the early drier part of the year, which coincided with the peak in malaria cases. both an ... | 1997 | 9088435 |
| synthetic dna-based genetic markers reveal intra- and inter-species dna sequence variability in the bubalus bubalis and related genomes. | a series of synthetic oligonucleotide probes were developed as markers for genetic analysis and molecular systematics of bubalus bubalis and other eutherian mammals. a very high level of heterozygosity (approximately 95%) was observed in the bubaline genome, with an overall band-sharing probability of 2.08 x 10(-11) using (tgg)6 and hinf i probe-enzyme combination. breed affiliation studies on different buffalo breeds, viz. toda, surti, mehsana, and murrah, revealed that the semiwild toda breed ... | 1997 | 9115646 |
| infectivity assays of foot-and-mouth disease virus: contact transmission between cattle and buffalo (bubalus bubalis) in the early stages of infection. | no differences were observed between cattle and indian buffalo (bubalus bubalis) in terms of temperature, viraemia or virus replication in the pharyngeal area, during the acute phase of foot-and-mouth disease. like cattle, the indian buffalo became infected and excreted virus before any clinical signs of foot-and-mouth disease developed. the disease was transmitted from cattle to buffalo and vice versa, during the acute stage of infection, as if the animals had been of the same species, presumab ... | 1997 | 9123797 |
| genetic variation within and relationships among populations of asian water buffalo (bubalus bubalis). | genetic variation at 53 protein-coding loci (25 polymorphic) was analysed for 17 water buffalo populations-12 swamp, three lankan and two of the murrah breed (river type), to determine the magnitude of genetic differentiation and the genetic relationships among the populations. in accord with previous cytological studies, the lankan buffalo clearly are river type. significant deviations from hardy-weinberg equilibrium were shown for a number of locus-population combinations, with all populations ... | 1997 | 9124701 |
| phylogenetic relationship among all living species of the genus bubalus based on dna sequences of the cytochrome b gene. | the cytochrome b genes of all living species of bubalus, including the river type and the swamp type of domestic buffaloes (bubalus bubalis), were sequenced to clarify their phylogenetic relationships. these sequences were compared together with the african buffalo (syncerus caffer) and banteng (bos javanicus) sequences as an outgroup. phylogenetic trees of bubalus species based on the dna sequences of the cytochrome b gene demonstrated that the tamaraw (bubalus mindorensis), endemic to the phil ... | 1996 | 9126673 |
| histamine h2 receptor mediated relaxation of buffalo (bubalus bubalus) ureter. | on the buffalo ureter, histamine did not elicit any direct effect. however, it caused concentration-dependent relaxation of the tissues precontracted by carbachol, phenylephrine, norepinephrine, kci or bacl2 and also inhibited the contractile effect of carbachol. metiamide selectively antagonised the relaxation and inhibition of contractile response but mepyramine did not show this effect. isoprenaline, dobutamine, salbutamol, verapamil and papaverine neither produced any direct effect nor relax ... | 1995 | 9135674 |
| a preliminary comparison of plasma fibrinogen concentrations, leukocyte numbers and erythrocyte sedimentation rate as non-specific indicators of inflammatory conditions in buffalo (bubalis bubalis). | the plasma fibrinogen concentration (fib), total leukocyte count (tlc), neutrophil, lymphocyte and monocyte numbers, and the erythrocyte sedimentation rate (esr) were determined in 153 buffaloes suffering from different clinical conditions. fib increased significantly (p < 0.05) in chronic mastitis, pyrexia, pyometra, cutaneous abscesses, tail gangrene and acute indigestion, whereas in most of the other conditions studied it varied non-significantly. tlc increased significantly in chronic mastit ... | 1997 | 9151410 |
| chromosomal assignment of six muscle-specific genes in cattle. | six genes expressed in skeletal or smooth muscle were assigned to bovine chromosomes using rodent, human or bovine cdna probes. myogenic determination factor (myod1) was 100% concordant with bos taurus chromosome (bta) 15, and myogenin (myog) was 95% concordant with bta 16. smooth muscle caldesmon (cald1) and the skeletal muscle chloride channel gene (clcn1) were 100% concordant with bta 4. myogenic factor 5 (myf5) was 90% concordant with bta 5; this assignment was confirmed by fluorescence in s ... | 1997 | 9172305 |
| genetic diversity of asian water buffalo (bubalus bubalis): microsatellite variation and a comparison with protein-coding loci. | twenty-one microsatellite loci in 11 populations of asian water buffalo (eight swamp, three river type) were analysed and, within and among populations, genetic variability was compared with results from 25 polymorphic protein-coding loci. within-population mean heterozygosity ranged from 0.380-0.615, approximately twice that estimated from the protein-coding loci (0.184-0.346). only eight significant departures from hardy-weinberg equilibrium (involving four loci) were detected; global tests sh ... | 1997 | 9172308 |
| characterization of strains of leuconostoc mesenteroides by analysis of soluble whole-cell protein pattern, dna fingerprinting and restriction of ribosomal dna. | of 215 leuconostocs isolated from field grass, natural whey cultures and water-buffalo milk, 178 were identified as leuconostoc mesenteroides ssp. mesenteroides while 37 strains could not be identified. biochemical characterization allowed seven groups to be defined. representative strains of each group and different habitat and nine reference strains were selected for further analyses. protein profiles appeared suitable for species discrimination, but did not differentiate between the three sub ... | 1997 | 9172399 |
| the effect of a combined approach to schistosomiasis control on the transmission of schistosoma japonicum in xingzi of poyang lake area, china. | the impact of a combined approach to schistosomiasis control from 1987 to 1989 and mass chemotherapy from 1992 to 1994 was studied in a rural community in xingzi county in the northwest corner of poyang lake in jiangxi province, china. humans, cattle, buffalo and pigs were known potential reservoirs of schistosoma japonicum. transmission occurs during water contact on and around seasonally flooded marshes that are used for grazing, the harvesting of grass and fishing. humans and livestock underw ... | 1996 | 9185265 |
| sarcocystis buffalonis n.sp. (protozoa: sarcocystidae) from the water buffalo (bubalus bubalis) in vietnam. | sarcocystis buffalonis n. sp. is proposed for a species forming thick-walled, macroscopic sarcocysts in skeletal muscles and the esophagus of the water buffalo (bubalus bubalis). sarcocysts of s. buffalonis were found in 68 (10.5%) of 647 buffalo carcasses examined grossly at slaughter in ho chi minh city in southern vietnam. sarcocystis buffalonis sarcocysts were 1-8 mm long and 0.1-0.5 mm wide. the cyst wall was 3-7.7 microns thick and had palisadelike villar protrusions that were constricted ... | 1997 | 9194830 |
| an apparent effect of immunopotentiation during late gestation on the postpartum reproductive performance of nili-ravi buffaloes (bubalus bubalis). | thirty-two nili-ravi buffaloes were used to determine the effect of prepartum immunopotentiation in late gestation with levamisole hydrochloride (0.5 mg/kg), vitamin e+selenium (vite-se) (etosol-se, 10 ml intramuscularly) or bacille calmette guerin (bcg) (0.5 ml/animal, subcutaneously) on postpartum reproductive performance. the immunopotentiating treatment was given twice, with treatments one week apart, approximately 80 days prior to the expected date of parturition. prepartum treatment with v ... | 1997 | 9232782 |
| chromosomal localization of alpha-galactosyltransferase 1 (ggta1) and elongation factor 2 (eef2) genes in river buffalo by fish. | | 1997 | 9244456 |
| comparative fish-mapping of villin (vil) gene in river buffalo, sheep and goat chromosomes. | two genomic clones of the villin (vil) gene were independently hybridized on river buffalo (bubalus bubalis, bbu), sheep (ovis aries, oar) and goat (capra hircus, chi) chromosomes by using sequential fluorescence in situ hybridization (fish) and r-banding (rbp- and rba-banding). clear hybridization signals revealed that vil is located in bbu 2q33, oar 2q33 and chi 2q33. these chromosomes and chromosome bands are believed to be homologous and the vil locus is the same as that previously found on ... | 1997 | 9246414 |
| fish mapping of three bovine cosmids to cattle, goat, sheep and buffalo x chromosomes. | contrary to extensive banding similarities of the autosomes, there are variations in the morphology as well as banding patterns of the x chromosomes of various species of bovidae family. we used three bovine x chromosome specific cosmid clones for fluorescence in situ hybridization mapping on cattle, goat, sheep, and river buffalo chromosomes to identify homologous regions in the genomes of the four species. the three cosmids (ciobt 314, 945, and 1489) mapped to well spaced locations on buffalo ... | 1997 | 9267181 |
| fish mapping of bovine u21, u1 and u7 molecular markers to river buffalo chromosomes 3p, 5q and 5p. | three bovine cosmid-derived microsatellites (idv-ga49, idvga7 and idvga47), previously assigned to cattle syntenic groups u1, u7 and u21, respectively, were fluorescence in situ hybridization (fish) mapped to river buffalo (bubalus bubalis, l., 2n = 50) chromosomes (bbu) 3p22 (idvga47, u21), bbu 5q21 (idvga49, u1) and bbu 5p19 (idvga7, u7) using sequential fish and r-banding techniques. these localizations allowed the assignment, for the first time, of the bovine syntenic groups u21, u1 and u7 t ... | 1997 | 9292239 |
| chemical composition and microstructure of uroliths and urinary sediment crystals associated with the feeding of high-level cottonseed meal diet to water buffalo calves. | the chemical composition and microstructure of seven uroliths and four urinary sediment samples associated with the feeding of high-level cottonseed meal diet to buffalo calves were examined by chemical qualitative analyses, scanning electron microscopy (sem), x-ray diffraction, and x-ray energy dispersive spectrometry (eds). struvite was a major component of kidney stones and of some bladder stones. the kidney stone sample appeared cracked under low power under sem, aggregated into tiny balls u ... | 1997 | 9300548 |
| identification and differentiation of bear bile used in medicinal products in taiwan. | one hundred eighty-three suspect bear bile used in medicinal products, collected in taiwan as gall bladders or dried powder forms, were analyzed using ftir, hptlc, and hplc techniques to identify whether they are indeed bear bile. those confirmed were further examined to determine whether the observed analytical parameters can be reliably used for source inference, i.e., differentiating products among north american black bear, farmed asiatic black bear, polar bear, etc. our data suggested that ... | 1997 | 9304828 |
| effect of recombinant bovine somatotropin and calcium salts of long-chain fatty acids on milk from italian buffalo. | fifty-one lactating italian river buffalo were used in an 84-d study to evaluate the effects of recombinant bovine somatotropin (bst) and ca salts of long-chain fatty acids on productive performance. treatments were 1) control diet, 2) the control diet plus 0.3 kg/d of added ca salts of long-chain fatty acids, 3) the control diet plus 320 mg of recombinant bst injected every 21 d for four cycles, and 4) the control diet plus 0.3 kg/d of added ca salts of long-chain fatty acids and 320 mg of reco ... | 1997 | 9313156 |
| effects of estradiol-17 beta and hcg supplementation on superovulatory responses and embryo quality in swamp buffalo (bubalus bubalis) implanted with norgestomet. | twenty-four cycling swamp buffaloes with normal reproductive histories and 2-3 months postpartum were used to investigate the effect of addition of estradiol-17 beta and human chorionic gonadotrophin (hcg) to the superovulation regime on the level of ovarian stimulation and embryo production. the estrous cycles of buffaloes were synchronized by prostaglandin injection and then divided into two groups for superovulatory treatment. those in group 1 (n = 12) received a implant containing 3 mg norge ... | 1997 | 9329859 |
| redescription of sarcocystis levinei dissanaike and kan, 1978 (protozoa: sarcocystidae) of the water buffalo (bubalus bubalis). | sarcocystis levinei dissanaike and kan, 1978, is redescribed because the original description was a mixture of 2 species, the amended s. levinei and the newly recognized s. buffalonis huong, dubey, nikkilä, and uggla, 1997. in histological sections, s. levinei sarcocysts are microscopic, up to 640 microm long and up to 95 microm wide. ultrastructurally, the cyst wall is thin (< 1.0 microm thick) with a minute undulating surface and smooth, hairlike villar protrusions arising at irregular distanc ... | 1997 | 9406792 |
| glucose metabolism in the erythrocytes of the buffalo (bubalus bubalis). | 14co2 production from [1-14c] glucose, the rate of glycolysis measured by the value of lactate production and the activities of various enzymes were determined in buffalo erythrocytes. buffalo red cell glycolytic metabolites were estimated and used for the calculation of the mass action ratios of reactions catalyzed by the glycolytic enzymes of bubalus bubalis. a comparison of the values of the mass action ratios with the equilibrium constants of the various glycolytic reactions indicate that he ... | 1997 | 9418003 |
| isolation of pestes des petits ruminants virus from an outbreak in indian buffalo (bubalus bubalis). | | 1997 | 9423239 |
| polymorphism of mitochondrial dnas of yunnan domestic water buffaloes, bubalus bubalis, in china, based on restriction endonuclease cleavage patterns. | restriction endonuclease cleavage patterns of mitochondrial dna(mtdna) of three local types of yunnan native water buffalo were analyzed using 18 enzymes which recognize six nucleotides. among the 12 animals analyzed, 3 of 18 enzymes, bamhi, ecori, and scal, revealed polymorphisms. three mtdna types were identified. the results indicate that a relatively low level of mtdna variation exists in yunnan domestic water buffaloes. the origin of chinese buffalo derived from yunnan province of china is ... | 1997 | 9435943 |
| purification and properties of buffalo (bubalus bubalis) erythrocyte hexokinase. | buffalo erythrocytes contain one isozyme of hexokinase that apparently lacks microheterogeneity as shown by chromatographic properties. a single protein band was detected by means of western blotting using an antibody raised in rabbits against homogeneous rat brain hexokinase i. the native protein has a molecular weight of 200,000 +/- 2880 by gel filtration. partial purification of erythrocyte hexokinase by a combination of several procedures, including affinity chromatography, which was previou ... | 1997 | 9440232 |
| hydrofluorosis in water buffalo (bubalus bubalis) in india. | the concentration of fluoride was determined in water, forage and urine and serum samples of buffaloes from the unnao district of india. the water and forage samples contained 2.01 +/- 0.51 and 22.50 +/- 0.82 ppm of fluoride, respectively. the analysis of biosamples collected from the affected animals revealed higher levels of fluoride in serum (0.58 +/- 0.05 ppm) and urine (10.64 +/- 1.23 ppm). clinical examination identified a 40.34% prevalence rate of clinical lesions suggestive of fluorosis ... | 1997 | 9447740 |
| six bovine cosmid-derived microsatellites mapping different syntenic groups are fluorescence in situ hybridization mapped to six river buffalo chromosomes. | six bovine cosmid-derived microsatellites (idvga53, bta3/u6; idvga61, u13; idvga41, bta12/u27; idvga32, bta15/u19; idvga59, bta26/u26 and idvga71, u8), previously assigned to cattle chromosomes, were fish-mapped to river buffalo chromosomes (bbu) 6q15, 8q34, 13q15, 16q25, 23q22 and 24q13 respectively. sequential fish/rba-banding allowed the precise identification of chromosomes and localization of probe-signals on chromosome bands. these localizations allowed us to assign indirectly, for the fir ... | 1997 | 9451954 |
| fish mapping of bovine zinc finger protein 164 (u23) and x81804 (u9) genes to river buffalo chromosomes 17 and 18 respectively. | | 1997 | 9451958 |
| parentage assessment of an ivf calf from bubalus bubalis by dna fingerprinting. | we used two synthetic oligodeoxyribonucleotide probes, oat18 and oms1 comprising (tgg)6 and (ggta)4 repeat motifs, respectively, in combination with hinfi enzyme for dna fingerprinting of a calf born from embryo transfer technology, its surrogate mother (sm) and the sperm donor (bull). of the two polymorphic probes, oat18 was found to be more informative for correctly establishing the parentage status of the in-vitro fertilized (ivf) calf and demonstrated the genetic relationship of the sm with ... | 1998 | 9469934 |
| spontaneous rate of sister chromatid exchanges (sces) in mitotic chromosomes of sheep (ovis aries l.) and comparison with cattle (bos taurus l.), goat (capra hircus l.) and river buffalo (bubalus bubalis l.). | the spontaneous level of sister chromatid exchanges (sces) in the sheep, estimated by exposing peripheral blood lymphocytes in 0.1 microgram/ml of 5'-bromodeoxyuridine (brdu), was 4.08 +/- 2.47 sce/cell, 2.04 sce/cell cycle, 0.038 sce/chromosome. the dose-response relationships, observed by exposing the cells to 0.1, 0.25, 0.5, 1.0, 2.5, and 5.0 micrograms/ml of brdu, rose rapidly from 0.1 to 0.25 microgram/ml, and less rapidly at higher concentrations, thus reaching a saturation level. the anal ... | 1997 | 9474905 |
| high salt method: a simple and rapid procedure for isolation of genomic dna from buffalo (bubalus bubalis) white blood cells. | three different methods, namely guanidine hydrochloride, phenol-chloroform extraction and high salt method, were compared in order to develop a simple method for the extraction of high yield of dna from buffalo blood suitable for genome analysis. both phenol-chloroform and high salt methods produced good yields of high molecular weight dna as determined by agarose gel electrophoresis. the yield and quality of dna extracted by high salt method was comparable to that of phenol-chloroform method. t ... | 1997 | 9475068 |
| biochemical responses in mixed chronic psoroptic and sarcoptic mange of buffaloes (bubalus bubalis). | | 1997 | 9493304 |
| simultaneous identification of antibodies to brucella abortus and staphylococcus aureus in milk samples by flow cytometry. | two flow cytometric assays are described herein. the single cytometric test (sct) detects antibodies to either brucella abortus or staphylococcus aureus in the serum or milk of a cow or water buffalo. the double cytometric test (dct) detects both anti-b. abortus and anti-s. aureus antibodies concurrently. in the sct, the sample to be tested is incubated in succession with the antigen (either b. abortus or s. aureus) and the proper secondary antiserum (fluorescein isothiocyanate-labelled rabbit a ... | 1998 | 9508316 |
| effect of culture conditions on the hatching ability of in vitro produced buffalo (bubalus bubalis) embryos. | | 1998 | 9519431 |
| zfx and zfy loci in water buffalo (bubalus bubalis): potential for sex identification. | in the present study a small region of zfx and zfy loci in buffalo have been amplified by polymerase chain reaction (pcr). restriction fragment length polymorphism (rflp) was also uncovered that can distinguish between male and female buffalo dna. the study also found a second copy of the zfx loci (zfxr) present in both male and female. sequence analysis showed that zfxr has a single base deletion that results in a redundant putative protein. | 1998 | 9526699 |
| seroprevalence of antibodies to neospora caninum and toxoplasma gondii in water buffaloes (bubalus bubalis) from egypt. | sera from 75 water buffaloes from egypt were examined using a direct agglutination test incorporating mercaptoethanol for antibodies to neospora caninum and toxoplasma gondii. antibodies to n. caninum were found in 51 (68%) of 75 buffaloes in titres of 1:20 (six buffaloes), 1:40 (15 buffaloes), 1:160 (one buffalo), 1:320 (one buffalo) and > or = 1:640 (28 buffaloes), using n. caninum formalin-preserved whole tachyzoites as antigen. antibodies to t. gondii were not found in a 1:100 dilution of se ... | 1998 | 9559371 |
| prevalence of antibodies to neospora caninum and toxoplasma gondii in cattle and water buffaloes in southern vietnam. | serum samples from 200 dairy cattle and 200 beef water buffaloes were collected in southern vietnam during may to september 1995. the sera were analysed for antibodies to neospora caninum by an enzyme-linked immunosorbent assay and the indirect fluorescent antibody test, and for antibodies to toxoplasma gondii by the direct agglutination test. significant levels of n. caninum antibodies were detected in 5.5% of the cattle sera and in 1.5% of the water buffalo sera. 10.5% of the cattle sera and 3 ... | 1998 | 9566094 |
| nocardia asteroides as a cause of pneumonia in a buffalo calf. | pulmonary nocardiosis has been described in a four-month-old male buffalo calf (bubalus bubalis) with a history of respiratory distress lasting ten days. microscopic examination of tissue imprints from the infected lungs revealed numerous gram-positive, acid-fast, fine, branched, filamentous organisms which were morphologically identical with those of nocardia spp. cultures of pneumonic lung specimens on plain sabouraud medium yielded pure growth of nocardia asteroides at 37 degrees c. lungs fro ... | 1997 | 9567313 |
| the potential of matrix-assisted laser desorption/ionization mass spectrometry in the quality control of water buffalo mozzarella cheese. | adulteration by addition of bovine milk to water buffalo milk employed for mozzarella cheese production is often observed. water buffalo milk and mozzarella cheese were analysed by matrix-assisted laser desorption/ionization mass spectrometry in order to achieve their rapid and accurate characterization and to evaluate possible fraudulence in mozzarella cheese production. | 1998 | 9654754 |
| trematode hemoglobins show exceptionally high oxygen affinity. | ligand binding studies were made with hemoglobin (hb) isolated from trematode species gastrothylax crumenifer (gc), paramphistomum epiclitum (pe), explanatum explanatum (ee), parasitic worms of water buffalo bubalus bubalis, and isoparorchis hypselobagri (ih) parasitic in the catfish wallago attu. the kinetics of oxygen and carbon monoxide binding show very fast association rates. whereas oxygen can be displaced on a millisecond time scale from human hb at 25 degrees c, the dissociation of oxyge ... | 1998 | 9675199 |
| igf-ii stimulation of in vitro maturation, in vitro fertilisation and subsequent development of buffalo (bubalus bubalis) oocytes in vitro. | | 1998 | 9682434 |
| a microsatellite sequence showing polymorphism in sheep, goat, cattle and water buffalo. | | 1998 | 9682454 |
| ontogeny, postnatal development and ageing of endocrine pancreas in bubalus bubalis. | the ontogenesis, postnatal development and ageing of the endocrine pancreas in mammals have not been extensively studied. in order to improve understanding of this organ, we studied the buffalo pancreas during fetal and postnatal development. glucagon, insulin and somatostatin immunoreactive cells (i.c.) were first seen in 2-mo-old embryos. pancreatic polypeptide (pp) i.c. were observed during the 3rd month of gestation. the early embryo pancreas was almost totally composed of endocrine tissue. ... | 1998 | 9688507 |
| kappa-casein polymorphisms in indian dairy cattle and buffalo: a new genetic variant in buffalo. | we screened 57 sahiwal cattle (bos indicus) and 53 murrah, 19 nili-ravi and 11 egyptian buffaloes (bubalus bubalis) to detect the polymorphisms at kappa-casein (csn3) gene using the polymerase chain reaction (pcr). csn3 a and b alleles were identified by pcr-rflp using the restriction enzymes that detect the underlying nucleotide changes at codon 136 (taql) and at codon 148 (hindiii or hinfi) in cattle. the frequency of csn3 b allele in the sahiwal cattle was estimated as 0.16 with no homozygous ... | 1998 | 9713674 |
| glycoconjugates in small antral ovarian follicles of the river buffalo (bubalus bubalis l.). | types and distribution patterns of glycoconjugates in antral ovarian follicles were investigated in the buffalo, using periodic-acid schiff (pas), high iron diamine (hid), low ion diamine (lid) and lectin histochemical staining methods. hid and lid staining procedures were preceded in some cases by digestion with testicular hyaluronidase, streptomyces hyaluronidase, chondroitinase abc and heparitinase (heparinase iii). lectin staining was performed with the use of 12 horseradish peroxidase (hrp) ... | 1998 | 9717561 |
| comparative fish-mapping of the prion protein gene (prnp) on cattle, river buffalo, sheep and goat chromosomes. | comparative fish-mapping of the prion protein gene (prnp) was performed on cattle (bta), river buffalo (bbu), sheep (oar) and goat (chi) chromosomes using a pcr-product as a probe and r-banding. prnp was mapped to bta13q17, bbu14q15, oar13q15 and chi13q15 according to standard nomenclatures. these chromosomes and bands were homoeologous among the four species, confirming the high degree of gene and chromosome banding conservation among bovids. furthermore, the assignment of prnp to river buffalo ... | 1998 | 9730603 |
| genetic diversity of asian water buffalo (bubalus bubalis): mitochondrial dna d-loop and cytochrome b sequence variation. | swamp and river buffalo mitochondrial dna (mtdna) was sequenced for 303 bp of the cytochrome b gene for 54 animals from 14 populations, and for 158 bp of the d-loop region for 80 animals from 11 populations. only one cytochrome b haplotype was found in river buffalo. of the four haplotypes identified in swamp buffalo, one found in all populations is apparently ancestral both to the other swamp haplotypes and to the river haplotype. the phylogenetic relationships among the 33 d-loop haplotypes, w ... | 1998 | 9745663 |
| in vitro maturation and fertilization, and subsequent development of buffalo (bubalus bubalis) embryos: effects of oocyte quality and type of serum. | in experiment 1, to determine the developmental potential of buffalo oocytes of different qualities, compact cumulus-oocyte complexes (cocs) with an unexpanded cumulus mass, and with homogeneous ooplasm were classified as grade 1 (with > or =5 layers of cumulus cells) and grade 2 less than 4 layers of cumulus cells). grade-3 oocytes were either without cumulus cells or with expanded cumulus mass, and with irregular ooplasm. the oocytes were matured for 24 h at 38.5 degrees c, 5% co2 in air in ma ... | 1998 | 9801269 |
| follicular fluid immunoreactive-inhibin concentrations in relation to follicular diameter and estradiol-17 beta, progesterone and testosterone concentrations in individual ovarian follicles in buffalo, bubalus bubalis. | the objective of this study was to measure the concentrations of immunoreactive inhibin (ir-inhibin) in follicular fluid from individual ovarian follicles and relate them to follicular diameter and follicular fluid concentrations of estradiol-17 beta, progesterone and testosterone in buffalo. follicular size was measured with an ultrasound machine and follicles were categorized as small (4 to 5 mm diam.), medium (6 to 9 mm diam.) and large (10 mm and above in diam.). ir-inhibin concentrations va ... | 1998 | 9838877 |
| reversal of sedative and clinicophysiological effects of epidural xylazine and detomidine with atipamezole and yohimbine in buffaloes (bubalus bubalis). | the sedative and clinicophysiological effects of either xylazine (0.1 mg/kg) or detomidine (50 micrograms/kg) administered epidurally to groups of five buffaloes were reversed by the intravenous administration of either yohimbine (0.125 mg/kg) or atipamezole (10 micrograms/kg). epidural xylazine or detomidine produced bradycardia, respiratory depression, ruminal stasis and hypothermia and these effects were completely reversed within five to 15 minutes after the intravenous administration of ati ... | 1998 | 9839365 |
| a serological survey of bovine brucellosis in india. | a serological survey of brucellosis in cattle and buffalo was performed in 23 states of india. a total of 30,437 bovine samples, comprising 23,284 cattle and 7,153 buffalo (bubalus bubalis), were screened. the screening initially used the rose bengal plate test: doubtful and positive samples were then titrated in the serum tube agglutination test. the overall prevalence rate of antibodies was 1.9% in cattle and 1.8% in buffalo. in a detailed study of 47 organised farms in the southern state of k ... | 1998 | 9850550 |
| zoo-fish and r-banding reveal extensive conservation of human chromosome regions in euchromatic regions of river buffalo chromosomes. | commercially available human chromosome (hsa) painting probes were hybridized on river buffalo (bubalus bubalis, 2n = 50) chromosomes by using fish and r-banding techniques. clear hybridization fitc-signals revealed extensive conservation of human chromosome regions in this species and demonstrated that human chromosome probes primarily paint euchromatic regions (r-bands). the present results are discussed in the light of previous gene mapping data obtained in river buffalo and zoo-fish data in ... | 1998 | 9858819 |
| abortion in water buffalo (bubalus bubalis) associated with bacillus licheniformis. | | 1998 | 9881445 |
| immunohistochemical study of endocrine cells in the gastrointestinal tract of the philippine carabao (bubalus bubalis). | the distribution and frequency of occurrence of endocrine cells in the gastrointestinal tract of the philippine carabao (bubalus bubalis) were studied by immunohistochemistry. fourteen types of immunoreactive (ir) endocrine cells were revealed. among the cell types, only chromogranin, serotonin, and bovine pancreatic polypeptide (bpp) were present in the entire gut, while the others showed restricted distribution: somatostatin, gastrin, and cholecystokinin in the abomasum and small intestine; me ... | 1998 | 9972649 |
| the primary structure of water buffalo alpha(s1)- and beta-casein identification of phosphorylation sites and characterization of a novel beta-casein variant. | the primary structure of water buffalo alpha(s1)-casein and of beta-casein a and b variants has been determined using a combination of mass spectrometry and edman degradation procedures. the phosphorylated residues were localized on the tryptic phosphopeptides after performing a beta-elimination/thiol derivatization. water buffalo alpha(s1)-casein, resolved in three discrete bands by isoelectric focusing, was found to consist of a single protein containing eight, seven, or six phosphate groups. ... | 1998 | 9988530 |
| eight molecular markers from bovine syntenic groups u2, u5, u24, u14, u12, u28, x and y were fluorescence in situ mapped to eight river buffalo chromosomes. | | 1998 | 10099882 |
| functional differentiation in trematode hemoglobin isoforms. | the hbs and the major electrophoretic hb components (isohbs) were isolated from three species of the trematodes, explanatum explanatum (ee), gastrothylax crumenifer (gc) and paramphistomum epiclitum (pe), that parasitise the common indian water buffalo bubalus bubalis. the hbs are monomeric and resemble the so-called nonfunctional mutant hemoglobins that have tyr at b10 or e7 positions (replacing leu and the his residues, respectively). however, they are capable of binding with o2 and co. o2 equ ... | 1999 | 10103000 |
| sarcocystis dubeyi n. sp. (protozoa: sarcocystidae) in the water buffalo (bubalus bubalis). | sarcocystis dubeyi n. sp. is proposed for a species forming thick-walled, microscopic sarcocysts in striated muscular tissues of the water buffalo (bubalus bubalis). sarcocysts of s. dubeyi were found in histological sections of skeletal muscles and esophagus, but not in heart and tongue, of 8 (13%) of 60 water buffaloes examined in ho chi minh city, vietnam. sarcocysts of s. dubeyi were up to 600 microns long and up to 200 microns wide. the cyst wall was 4.5-9 microns thick and was composed of ... | 1999 | 10207372 |
| an evaluation of tuberculin skin tests used to diagnose tuberculosis in swamp buffaloes (bubalus bubalis). | three types of tuberculin tests were evaluated in swamp buffaloes in two herds of known tuberculosis-positive and tuberculosis-negative status, and the results were compared with postmortem records of 73 tuberculous and 12 non-tuberculous buffaloes from a different herd. of the 73 tuberculous swamp buffaloes, 30 (41%), 46 (63%) and 56 (77%) animals were detected by the caudal-fold skin test, the cervical skin test and the stormont test, respectively. of the 12 non-tuberculous buffaloes, 5 (42%), ... | 1999 | 10223316 |
| seminal-type ribonuclease genes in ruminants, sequence conservation without protein expression? | bovine seminal ribonuclease (bs-rnase) is an interesting enzyme both for functional and structural reasons. the enzyme is the product of a gene duplication that occurred in an ancestral ruminant. it is possible to demonstrate the presence of seminal-type genes in all other investigated ruminant species, but they are not expressed and show features of pseudogenes. in this paper we report the determination of two pancreatic and one seminal-type ribonuclease gene sequences of swamp-type water buffa ... | 1999 | 10231579 |
| characterization and chromosomal distribution of satellite dna sequences of the water buffalo (bubalus bubalis). | satellite dna sequences were isolated from the water buffalo (bubalus bubalis) after digestion with two restriction endonucleases, bamhi and stui. these satellite dnas of the water buffalo were classified into two types by sequence analysis: one had an approximately 1,400 bp tandem repeat unit with 79% similarity to the bovine satellite i dna; the other had an approximately 700 bp tandem repeat unit with 81% similarity to the bovine satellite ii dna. the chromosomal distribution of the satellite ... | 1999 | 10355126 |
| atypical cilia in the tracheal epithelium of healthy water buffaloes (bubalus bubalis). | samples of tracheal mucosa were obtained from 10 healthy adult water buffaloes and 50 000 cilia were examined ultrastructurally. ciliary abnormalities were found in all 10 subjects. atypical cilia occurred as compound cilia (up to 1.5%), intracytoplasmic cilia (0.07%) and swollen cilia (0.05%). the microtubular pattern was determined in 5000 cross-sectioned cilia, with about 7% showing axonemal abnormalities in which peripheral defects prevailed. some electron-dense plugs appeared inside the cyl ... | 1999 | 10373294 |
| cdna cloning and sequence analysis of bubaline growth hormone. | the cdna for bubalus bubalis growth hormone (gh) has been cloned and sequence determined through rt-pcr approach. the nucleotide sequence of bubaline gh cdna was in a single reading frame coding for a protein of 191 residues comprising a putative signal sequence of 27 amino acids. homology comparison of the sequence with other mammalian gh cdnas showed a very high degree of evolutionary conservation. bubaline gh sequence shared a homology of 99.5%, 99.5%, 98.6%, 87.6% and 61.9% with that of ovin ... | 1999 | 10376211 |
| molecular cloning and sequence analysis of the cdna encoding beta-lactoglobulin in bubalus bubalis. | the cdna for bubaline beta-lactoglobulin (beta lg) has been cloned through rt-pcr approach and sequenced. sequence data showed a single open reading frame coding for a protein of 180 amino acids with a signal sequence of 18 amino acid residues. comparison with other ruminant beta lg sequences revealed a high homology indicating the protein to be conserved through evolution. the degree of homology, at amino acid level, is 96.1%, 95.6%, 93.9% and 63.7% with goat, sheep, cow and pig, respectively. | 1999 | 10376212 |
| comparison of the human with the sheep genomes by use of human chromosome-specific painting probes. | human chromosome specific painting probes were hybridized on sheep (ovis aries, 2n = 54) chromosomes by fish. the painting results on sequentially stained rba-banded preparations demonstrated high degree of conserved regions between human and sheep genomes. a total of 48 human chromosome segments were detected in sheep chromosomes. comparisons with sheep gene mapping data available and previous zoo-fish data obtained in sheep, cattle, and river buffalo were performed. | 1999 | 10384047 |
| health and growth of water-buffalo calves in nueva ecija, the philippines. | this prospective observational study was undertaken in the province of nueva ecija in the philippines to assess current levels of health and growth achieved by a defined cohort of water-buffalo calves raised by smallholder farmers and to identify factors associated with the performance of these animals during the first 6 months following birth. seventy two animals were enrolled, including 16 philippine native water-buffalo and 54 crossbred (f1, f2 or backcrosses) animals. dullness (which was ass ... | 1999 | 10384946 |
| analysis of conserved microsatellite sequences suggests closer relationship between water buffalo bubalus bubalis and sheep ovis aries. | the distribution and evolutionary pattern of the conserved microsatellite repeat sequences (ca)n, (tgg)6, and (ggat)4 were studied to determine the divergence time and phylogenetic position of the water buffalo, bubalus bubalis. the mean allelic frequencies of these repeat loci showed a high level of heterozygosity among the euartiodactyls (buffalo, cattle, sheep, and goat). genetic distances calculated from the allelic frequencies of these microsatellites were used to position bubalus bubalis i ... | 1999 | 10390160 |
| comparative fish-mapping of six expressed gene loci to river buffalo and sheep chromosomes. | six expressed gene loci (nf1, cryb1, chrnb1, tp53, p4hb and gh1), recently assigned to cattle chromosome 19 by both radiation hybrid analysis and fish-mapping, were comparatively fish-mapped to river buffalo chromosome (bbu) 3p and sheep chromosome (oar) 11, extending the physical map in these two important bovids. the six loci mapped to the same homoeologous chromosome bands of bbu 3p and oar 11, and their gene order was centromere-nf1-cryb1-chnrb1-tp53-(gh1, p4hb). | 1999 | 10393419 |
| antioxidant defense in the follicular fluid of water buffalo. | oxidative damages to the oocyte or follicular cells were suggested to trigger atresia. in water buffalo, loss of the blood-follicle barrier sieving effect on the diffusion of plasma haptoglobin was previously found to be associated with atretic oocytes. the redox status of water buffalo follicles was evaluated by measuring in follicular fluid both the total antioxidant capacity (tac), expressed as trolox equivalents, and the concentration of specific free radical scavengers, determined by high-p ... | 1999 | 10394004 |
| sequential clinical, haematological, biochemical, immunopathological and histopathological alterations in buffalo calves (bubalus bubalis) intravenously infected with absidia corymbifera. | absidiosis was produced experimentally in 18 buffalo calves by intravenous inoculation of spores of absidia corymbifera. infected animals exhibited dullness, depression, partial anorexia and an initial pyrexia and coughing during the first week and two animals died on each of 9, 13 and 16 days post infection (dpi). the haemoglobin concentration and total erythrocyte count showed no appreciable change from their basal values at any stage of the experiment. however, the erythrocyte sedimentation r ... | 1999 | 10445250 |
| ultrastructure and innervation of water buffalo (bubalus bubalis) seminal vesicle. | the lining epithelium of secretory end pieces and central glandular duct in the seminal vesicle of the water buffalo (bubalus bubalis) consists of columnar principal and small polymorphous basal cells. a system of intercellular and even intracellular canaliculi enlarges the secretory surface. the most prominent organelle of the columnar principal cells is the granular endoplasmic reticulum, forming large aggregates of parallel lamellae. using antibodies against the neural cell adhesion molecule ... | 1992 | 10457788 |
| laboratory production of buffalo (bubalus bubalis) embryos. | there is an increasing interest in large-scale in vitro production (ivp) of buffalo embryos through in vitro maturation (ivm), fertilization (ivf) and culture (ivc) of oocytes for faster multiplication of superior germplasm. the recovery of total and usable quality oocytes from slaughterhouse ovaries is low in this species. the nuclear maturation rates of buffalo oocytes matured in the presence of follicular fluid or serum and hormones like luteinizing hormone, follicle-stimulating hormone and o ... | 1998 | 10461670 |
| effect of pen size on behavioral, endocrine, and immune responses of water buffalo (bubalus bubalis) calves. | female water buffalo (bubalus bubalis) calves (n = 28) aged 7 to 10 d were divided into four groups of seven animals each to examine the effects of space allowance (group a: 2.6 indoor m2 + 2.0 outdoor m2/calf; group b: 2.6 indoor m2/calf; group c: 1.5 indoor m2/calf; group d: 1.0 indoor m2/calf) on behavioral, endocrine, and immune variables for a period of 60 d. animals were offered 7 l/d of a commercial acidified milk substitute. the calves averaged 45.9 kg initially and 92.4 kg finally. the ... | 1999 | 10461979 |
| paracrystalline structures in the epithelial principal cells of the epididymis of water buffalo (bubalus bubalis). | the ultrastructure of many principal cells in the cauda epididymis of water buffaloes with ages varying between 4, 18, 24, 30 and 36 months, revealed, in many cells, the presence of long, curved paracrystalline structures that are quite large and frequently encountered in the cytoplasm, usually near the nucleus. concomitantly or not, smaller rod-like, hexagonal or curled structures can be found in the nucleus. both structures, cytoplasmic and intranuclear, are made up of a sheath of parallel fil ... | 1999 | 10481305 |
| prevalence of sarcocystis spp. in water buffaloes in vietnam. | muscles from heart, tongue, oesophagus, neck and abdomen from 502 adult water buffaloes (bubalus bubalis) slaughtered in ho chi minh city, vietnam, between 1996 and 1997 were examined for sarcocystis cysts by a combination of ocular and histological examination. sarcocysts were present in 396 (79%) of the animals and the prevalence increased with age from a 57% infection rate among 2-3 year old animals to 93% among 6-7 year olds. the prevalence was higher in animals originating from the northern ... | 1999 | 10489200 |
| the ovarian response of prepubertal buffalo (bubalus bubalis) to superovulation with equine chorionic gonadotrophin with and without treatment with gnrh. | | 1999 | 10489273 |
| alternative nonallelic deletion is constitutive of ruminant alpha(s1)-casein. | multiple forms of alpha(s1)-casein were identified in the four major ruminant species by structural characterization of the protein fraction. while alpha(s1)-casein phenotypes were constituted by a mixture of at least seven molecular forms in ovine and caprine species, there were only two forms in bovine and water buffalo species. in ovine and caprine forms the main component corresponded to the 199-residue-long form, and the deleted proteins differed from the complete one by the absence of pept ... | 1999 | 10524777 |
| manipulation of milk production and quality by use of somatotropin in dairy ruminants other than cow. | the ability of recombinant bovine somatotropin (bst) to enhance milk production is well established in cows and in other dairy ruminants. in dairy ewes, we found increased milk yield (20-30%) following treatment with bst, which did not negatively affect the gross composition or coagulating properties of milk, except in the advanced stage of lactation, when the percentages of milk protein and fat were reduced and the coagulation time was improved (shorter) compared with untreated animals. in dair ... | 1999 | 10527116 |
| toward three-dimensional analysis of cochlear structure. | recent results from a three-dimensional model of the cochlea are summarized. the features include physically realistic values of basilar membrane stiffness, mass, and fluid viscosity. the simple 'feed-forward' principle for the active process yields results in qualitative agreement with recent measurements in the cochlea. the limitation is a simplified representation of the organ of corti, with two degrees of freedom representing the motion of the pectinate and arcuate zones of the basilar membr ... | 1999 | 10529645 |
| postnatal development of intestinal endocrine cell populations in the water buffalo. | the frequency and distribution of 11 endocrine cell populations were studied in the intestine of differently aged buffalo, grouped on the basis of diet: 2-d-olds (suckling), 5-mo-olds (weaning) and 5-y-olds (ruminant adult diet). the endocrine cell populations were identified immunocytochemically using antisera against 5-hydroxytryptamine (5-ht), somatostatin, gastrin, cholecystokinin (cck), cooh-terminal octapeptide of gastrin/cck, neurotensin, motilin, gastric inhibitory polypeptide (gip), sec ... | 1999 | 10580859 |
| suppressor cell activity of intestinal mucosal leucocytes from buffalo (bubalus bubalis arni). | suppressor activity of buffalo intestinal intraepithelial leucocytes and lamina propria leucocytes was induced by concanavalin a, and was assayed against the mitogenic response of autologous and allogenic leucocytes to mitogens. appreciable suppression was observed with 25 micrograms cona/ml on the proliferative activity of the responder cells cocultured at a ratio of < 2:1 (suppressor:responder cell). mitomycin c treatment of intestinal leucocytes did not totally vanish the viability and functi ... | 1999 | 10641159 |
| expression of growth factor ligand and receptor genes in preimplantation stage water buffalo (bubalus bubalis) embryos and oviduct epithelial cells. | the temporal pattern of expression of genes for several growth factor ligands and receptors was examined in preimplantation water buffalo embryos and oviduct epithelial cells using rt-pcr. the identity of the resulting pcr products was confirmed by their expected size, restriction analysis, southern blot hybridization and nucleotide sequence analysis. preimplantation stage embryos from the one-cell to the blastocyst stage were derived after maturation, fertilization and culture of oocytes in vit ... | 1999 | 10645246 |
| reproduction in water buffalo: comparative aspects and implications for management. | the domestic buffalo occupies an important niche in many ecologically disadvantaged agricultural systems, providing milk, meat and draught power. although buffalo can adapt to harsh environments and live on low quality forage, their reproductive efficiency is often compromised by such conditions. climatic stress depresses ovarian cyclicity, oestrous expression and conception rates. poor nutrition, usually related to seasonal fluctuations in availability and quality of feed, delays puberty and in ... | 1999 | 10692852 |
| species origin of milk in italian mozzarella and greek feta cheese. | several animal species such as cattle, goats, sheep, and water buffalo provide milk for dairy products. we describe a simple procedure for detecting the species origin of milk used for cheese production. dna was isolated from italian mozzarella or greek feta by sequential organic extractions and resin purification. this dna was analyzed by polymerase chain reaction-restriction fragment length polymorphism as described previously for meat samples. this procedure differentiated mozzarella made fro ... | 2000 | 10716574 |
| chromosomal localization of four hsa2 type i loci in river buffalo (bubalus bubalis, 2n = 50) chromosomes 2q and 12. | | 2000 | 10723732 |
| isolation of immunologically active uterine luminal proteins associated with follicular and luteal phases of the ovary in buffalo (bubalus bubalus). | uterine luminal proteins (ulp) collected from the genital tract of buffalo during the follicular (group f) and luteal (group l) phases of the estrous cycle were chromatographed using sephacryl s-200 gel. five peaks were detected in each group. different protein concentrations (10 to 200 microg) from peaks i and v in each group were examined for immunological activity on polymorph nuclear leukocytic cells (pmnl) in vitro. all concentrations except 10 microg of ulp peak i (< or = 250 kda) in group ... | 1999 | 10729036 |
| partial purification and characterization of gastrothylax crumenifer somatic antigens. | the soluble extracts of gastrothylax crumenifer isolated from the rumen of buffalo (bubalus bubalis) were fractionated on a sephadex g-200 column. a total of eight major fractions (f1, f2, f3, f4, f5, f6, f7, and f8) were separated from the whole homogenate of g. crumenifer, and each of these fractions was tested for their antigenicity by elisa against rabbit hyperimmune sera. it was observed that f1, f2, f3 and f4 were highly antigenic, f6 and f7 were moderately antigenic and f5 and f8 were poo ... | 2000 | 10729642 |
| cytogenetics of three breeds of river buffalo (bubalus bubalis l.), with evidence of a fragile site on the x chromosome. | the cytogenetic study of 182 river buffalo (bubalus bubalis l., 2n = 50) of murrah, mediterranean and jaffarabadi breeds, from the state of são paulo, was carried out to characterize their chromosomes and to detect possible chromosomal abnormalities. the karyotypes were indistinguishable with conventional staining as well as with c and replication r banding techniques. in about 44% of the sample (8 males and 72 females), an x marker chromosome due to a fragile site was shown. the frequency of me ... | 1998 | 10732032 |
| early development and location of embryos in the reproductive tract of nili ravi buffalo (bubalus bubalis): a retrospective analysis. | one year data on embryo recovery were analyzed to study the development and descent of preimplantation embryos in nili ravi water buffalo. forty-five superovulatory attempts were performed on 23 buffalo. a total of 45 embryos were recovered either nonsurgically or after slaughtering the animals at various time intervals (85 to 176 h) post estrus. embryos were located in the oviducts at 85 h after estrus. at 108 h post estrus, most of them (78%) were recovered from the uteri. the embryos had 8 to ... | 1998 | 10732056 |
| rate of transport and development of preimplantation embryo in the superovulated buffalo (bubalus bubalis). | this study was designed to ascertain the rate of transport and development of preimplantation embryo in the superovulated buffalo in order to determine the optimum time for their nonsurgical collection. eighteen murrah-type buffalo were superovulated with 600 mg nih-fsh-p1. luteolysis was induced by administration of pgf2 alpha at 72 (pg + 72) and 84 h (pg + 84) after initiating gonadotrophin treatment and fixed-time ai was done beginning at 36 h post pg + 72 administration and at 12-h intervals ... | 1998 | 10732154 |
| bull-specific effect on fertilization rate and viable embryo recovery in the superovulated buffalo (bubalus bubalis). | this study was conducted to compare fertilization rate and viable embryo recovery rate in superovulated buffalo (n = 64) following insemination with semen from buffalo bulls (n = 5) having different fertility rates as determined by ai. frozen-thawed semen from fertile bulls with similar post-thaw progressive motility and sperm morphology was used to inseminate buffalo at superovulatory estrus. fertilization and viable embryo recovery rates differed among bulls, but this bull-specific effect was ... | 1999 | 10734367 |
| effect of treatment with recombinant bovine somatotropin on responses to superovulatory treatment in swamp buffalo (bubalus bubalis). | the objective of this study was to determine the effect of treatment with recombinant bovine somatotropin (rbst) on the response to superovulatory treatment in swamp buffalo. estrous cycles of 16 buffalo cows were synchronized by intravaginal administration of progesterone and estradiol benzoate, and the cows were then randomly divided into 2 groups. the rbst-treated group received 250 mg of a sustained-release formula of rbst on day 4 after progesterone implantation, whereas the control group d ... | 1999 | 10734373 |
| factors affecting pregnancy rate following nonsurgical embryo transfer in buffalo (bubalus bubalis): a retrospective study. | the objectives of this study were to determine the pregnancy rate and factors affecting it following nonsurgical embryo transfer in buffalo. donor buffalo were superovulated with fsh, and embryos collected nonsurgically were evaluated for stage of development and quality. they were transferred nonsurgically to 91 recipients on days 5 to 7 of the natural (n = 52) or induced (n = 39) estrus (estrus = day 0). the overall pregnancy rate of 24/91(26.4%) was higher than in earlier reports for buffalo ... | 1999 | 10734401 |
| influence of cumulus cells and sperm concentration on cleavage rate and subsequent embryonic development of buffalo (bubalus bubalis) oocytes matured and fertilized in vitro. | the objective of the present study was to investigate the effects of sperm concentration and presence or absence of cumulus cells on fertilization, cleavage rate and subsequent embryonic development upto the blastocyst stage in buffalo. cumulus-oocyte-complexes (cocs) obtained from slaughterhouse ovaries were matured in vitro in tcm-199 + 10% fbs + 5 micrograms/ml fsh-p for 24 h. after maturation the cocs were either used as such (cumulus-intact) or freed from attached cumulus cells by repeated ... | 1998 | 10734439 |