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evaluation of an enzyme immunoassay test for the diagnosis of chlamydia psittaci infection in free-ranging koalas (phascolarctos cinereus) in southeastern queensland, australia.the ideia chlamydia test, a commercially available antigen-capture enzyme-linked immunosorbent assay (elisa) test, based on a monoclonal antibody for the detection of chlamydia in clinical specimens, was evaluated in a population of 65 free-ranging koalas in southeastern queensland determined to be infected with chlamydia psittaci. compared to isolation of the organism in tissue culture, the sensitivity of the ideia test ranged from 3 to 11%, and the specificity from 90 to 97%. the results indic ...19883373632
aspects of the epidemiology of chlamydia psittaci infection in a population of koalas (phascolarctos cinereus) in southeastern queensland, australia.a population of free-ranging koalas in southeastern queensland was examined to determine the prevalence of chlamydia psittaci infections. although c. psittaci was isolated from 46 of 65 (71%) koalas studied, only six (9%) of these had clinical signs of disease. most adult females (82%) had back or pouch young present even though 67% of them were infected. there were no significant correlations between age, sex or site of sampling (urogenital versus conjunctival tissues) and the isolation of c. p ...19883373633
developmental-form-specific dna-binding proteins in chlamydia spp.we identified dna-binding proteins specific to the elementary body (eb) developmental form of chlamydia spp. chlamydial proteins from whole lysates were separated by polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with nick-translated chlamydial dna. by this method, c. trachomatis serovar l2 ebs had three unique protein bands of 58,000, 25,700, and 17,000 molecular weight not seen in the reticulate bodies. the 17,000-molecular-weight protein and the 25,700-molecular ...19883384472
chlamydia psittaci: is tonsillar tissue the portal of entry in ovine enzootic abortion?ewes at 70 days of gestation were exposed by various routes to a culture of chlamydia psittaci. chlamydial infection of fetal tissues, generally accompanied by abortion, was observed only in four of six ewes inoculated via the tonsillar crypts and in five of seven ewes injected subcutaneously; most of these also developed antibodies to c psittaci. seroconversion after normal lambing was also observed in most ewes inoculated orally or by stomach tube, despite failure to detect chlamydia in them.19883387681
[detection of chlamydia psittaci in the feces of swine]. 19883390133
two distinct forms of chlamydia psittaci associated with disease and infertility in phascolarctos cinereus (koala).while several diseases associated with chlamydia psittaci infection have been reported in phascolarctos cinereus (koala), it is still unclear whether one or more chlamydial strains are responsible. in this study, we provide evidence, obtained by restriction enzyme and gene probe analysis, that two quite distinct strains of c. psittaci infect koalas; one strain was isolated from the conjunctivae, and the other was isolated from the urogenital tract and the rectum. a gene probe, pfen207, containin ...19883397180
[the occurrence of chlamydia psittaci in the genital tract and feces of slaughtered female cattle]. 19883420992
a field trial of ovine enzootic abortion vaccine. 19873424575
specific interaction of bovine igg1 and igg2 subclasses with different chlamydial antigens.antigens of the immunotype 1 strain b-577 of chlamydia psittaci, which were separated by sds-page and electrophoretically transferred to nitrocellulose membranes were used to probe sequential serum samples of cattle with experimentally induced or naturally occurring chlamydial infections. applying igg1- and igg2-specific markers in an enzyme immunoassay procedure, a predominance of igg2 reactions with different proteins was determined. the interaction of igg1 with antigens such as the genus-spec ...19873425033
[the occurrence of latent intestinal chlamydia psittaci infection in cattle]. 19873426536
[seropositivity in occupationally exposed persons to toxoplasmosis, q-fever and chlamydiosis]. 19873427492
[etiologic studies of abortion in sheep with special reference to detection of brucella, campylobacter, salmonella, listeria, leptospira and chlamydia]. 19873435407
detection of chlamydia psittaci from various animal species by reverse passive haemagglutination.a reverse passive haemagglutination test (rph) has previously been developed to detect the genus-specific antigen of chlamydia. clinical samples were obtained from various sites of different animal species. the rph test detected chlamydial antigen from clinical cases of conjunctivitis in cats, abortion in sheep and psittacosis in birds. although not as sensitive as cell culture isolation, this test has the advantages of rapidity and of detecting antigen from dead chlamydiae.19873439015
[the epizootic process in ewe lambs experimentally infected with salmonella abortus ovis and chlamydia psittaci var. ovis].studied was the dynamics of antibody production and salmonella shedding in a flock of year-old female sheep affected with a mixed form of infection of salmonella abortus ovis and chlamydia psittaci var. ovis. it was established that in the presence of a mixed infection dominating was the part played by chlamydia organisms. the apparent epizootic process with these agents usually ran its course in the later period of pregnancy, while salmonella organisms acted by the end of the first half of preg ...19873439023
the effect of intracellular chlamydia psittaci on lysosomal enzyme activities in mouse peritoneal macrophages.live or heat killed (30 min at 56 degrees c) chlamydia psittaci elementary bodies (eb) were phagocytosed by mouse peritoneal macrophages. inoculation with killed chlamydia caused rises in three lysosomal enzyme activities tested, especially in acid phosphatase activity. in contrast, after infection with live chlamydia, only a negligible increase was seen in acid phosphatase activity, and no change in the activities of beta-glucuronidase and cathepsin d was observed. it was concluded that regulat ...19873442238
[vaccination against bovine chlamydial abortion with a temperature-sensitive mutant of chlamydia psittaci].the safety of the vaccinal strain 1b was studied on cows during the pregnancy following the vaccination by recording complement fixing (cf) antibody titer, chlamydial excretion and calving performance. the immunity of vaccinated cows was challenged 19 months after vaccination by intradermal inoculation of 10(7) pfu of the virulent strain av1 during the 7th month of pregnancy.19873451688
antichlamydial activity of ofloxacin.the in vitro activity of ofloxacin, a new pyridone-carboxylic acid, against 11 strains of chlamydia trachomatis and six strains of chlamydia psittaci was determined. all test strains of both species were inhibited by 0.39 micrograms of ofloxacin per ml. the antichlamydial activity of ofloxacin was comparable to that of doxycycline and four- to eightfold less than that of minocycline. the results of this susceptibility test, coupled with those of previous pharmacokinetic analyses of ofloxacin, wa ...19863462450
ocular delayed hypersensitivity: a pathogenetic mechanism of chlamydial-conjunctivitis in guinea pigs.we used a naturally occurring, chlamydia psittaci-caused eye disease in guinea pigs, guinea pig inclusion conjunctivitis, as an animal model to understand both the immune response and the pathogenesis of chlamydial eye infections. when instilled into the conjunctival sac of guinea pigs that had been previously infected and were immune, viable chlamydiae or a triton x-100-soluble extract of them produced a short-lived (12-48 hr) eye disease indistinguishable clinically and histologically from tha ...19863463978
t cell mediated and humoral immunity in a mouse chlamydia psittaci systemic infection.immunity against chlamydia psittaci, an obligate intracellular parasite, was studied in a mouse model of systemic infection. sera (0.1 ml) and splenic cells (2 x 10(8)) from immunised mice were given intravenously to susceptible mice 16 hours before intravenous challenge with 1 x 10(5) plaque forming units (pfu) of virulent strain ab7. transfer of immune cells primed with virulent strain ab7 or vaccinal strain 1b, lowered splenic and hepatic colonisation by approximately 5.5 log pfu. treatment o ...19873498194
in-vitro activity of roxithromycin against respiratory and skin pathogens.the activity of roxithromycin was determined by a microdilution method, in comparison with erythromycin, spiramycin and josamycin. roxithromycin and erythromycin showed very similar mics against staphylococci, streptococcus pneumoniae, str. pyogenes and haemophilus influenzae. in most cases, spiramycin and josamycin appeared similarly or more active. the activity of roxithromycin against mycoplasma pneumoniae, legionella spp., chlamydia psittaci and, to some extent, against pasteurella spp. was ...19873501424
an enzyme-linked immunosorbent assay (elisa) for anti-chlamydial secretory immunoglobulin a in guinea pig tears.a method is described which permits the assay of specific secretory immunoglobulin a (siga) antibodies produced by guinea pigs in response to ocular infection with the guinea pig inclusion conjunctivitis strain of chlamydia psittaci (gpic agent). the enzyme-linked immunosorbent assay (elisa) developed was shown to be more sensitive and less subjective than the micro-immunofluorescence assay as a means of assaying specific antibody.19863511151
[keratoconjunctivitis caused by chlamydia psittaci].a 25-year-old female patient suffering from acute follicular keratoconjunctivitis with epithelial and subepithelial corneal infiltrates was examined. antibodies of the igm class directed against c. psittaci were found. infection due to c. trachomatis was ruled out by serology (elisa) and negative isolation attempts. the patient's cat had rhinitis and conjunctivitis and a positive c. psittaci specific igm antibody titer. systemic application of tetracycline resulted in fast resolution of symptoms ...19863515033
both species of chlamydia and two biovars of chlamydia trachomatis stimulate mouse b lymphocytes.we have investigated the ability of both species of chlamydiae (c. trachomatis and c. psittaci), two major biovars of c. trachomatis (lymphogranuloma venereum and trachoma), and the two developmental forms of chlamydia (reticulate and elementary bodies) to stimulate murine spleen lymphocytes. all of these forms of the bacteria induce potent proliferation and differentiation to plaque-forming cells by b lymphocytes in vitro. chlamydiae induce a broad antibody response, suggesting that stimulation ...19863517167
potential danger to pregnant women of chlamydia psittaci from sheep. 19863521058
etiologic significance of bacterial isolates from rams with palpable epididymitis.identified and partly identified bacterial isolates were obtained from 48 rams of various breeds that had unilateral or bilateral epididymitis. most of the animals were approximately 1 year of age; a few were older. brucella ovis, actinobacillus spp, corynebacterium spp, haemophilus spp, acinetobacter spp, escherichia coli, moraxella spp, staphylococcus spp, pasteurella spp, streptococcus spp, and chlamydia psittaci were isolated. a vaccine strain of b ovis, isolated species of bacteria, and mix ...19863521407
[chlamydia and urogenital chlamydioses (a review of the literature)]. 19863523978
eubacterial origin of chlamydiae.the sequence of the 16s rrna gene from chlamydia psittaci was determined. comparison of this sequence with other 16s rrna sequences showed the organism to be eubacterial. the organism represents a hitherto unrecognized major eubacterial group. however, this group may be peripherally related to the planctomyces and relatives. although these two groups seem to have very little in common phenotypically (they have been studied in very different ways), cell walls in both cases contain no peptidoglyca ...19863525517
bovine chlamydial abortion: serodiagnosis by modified complement-fixation and indirect inclusion fluorescence tests and enzyme-linked immunosorbent assay.sequential serum samples from 11 cows experimentally inoculated with different abortigenic strains of chlamydia psittaci were tested by a modified complement-fixation (mocf) test, an indirect inclusion fluorescence antibody (iifa) test, and an enzyme-linked immunosorbent assay (elisa). one of these cows was not pregnant, another gave birth at term to a healthy calf, and all the others prematurely delivered infected dead calves or weak live calves. the results achieved with these tests on sera of ...19863527000
[tolerance and immunization trials with a commercial vaccine against chlamydia psittaci and coxiella burnetii]. 19863527655
prevalence of legionnaires' disease in pneumonia patients admitted to a danish department of infectious diseases.during a 14-month study period, 92 patients admitted to the university clinic for infectious diseases with pneumonia were investigated to determine the prevalence and severity of legionnaires' disease (ld). the diagnosis of ld was based on positive serology. antibodies to 10 different legionella antigens--legionella pneumophila serogroups 1-6, fluoribacter (legionella) bozemanae, f. dumoffii, f. gormanii, and tatlockia (legionella) micdadei--were measured by the microagglutination (ma) and indir ...19863532303
propagation of ovine and bovine abortion strains of chlamydia psittaci in suspension cultures of l-cells. 19863535313
elisa for detection of human antibodies to chlamydiae.an enzyme-linked immunosorbent assay for the detection of human antibodies to chlamydiae is described which exploits the cross-react properties between the genus-specific antigen of chlamydiae and the relps constituent of the outer membrane of a salmonella minnesota mutant. of 100 random sera tested by elisa-relps and immunofluorescence 78% showed an absolute correlation, 15% were positive in immunofluorescence and negative in elisa and 7% were positive in elisa and negative in immunofluorescenc ...19863537133
effect of infection of the genital tract on the concentration of igg and albumin in bull serum and semen.bovine igg and albumin concentrations were determined from serum and semen of 59 bulls that were divided into 4 groups: 35 non-infected bulls (group 1); 10 with vesiculitis due to corynebacterium pyogenes (group 2); 10 bulls with orchitis due to chlamydia psittaci (group 3); and 4 bulls with infectious vesiculitis (group 4) sampled both before and after antibiotic treatment. serum igg concentrations (25 mg/ml approximately) were similar in non-infected (gp 1) and infected bulls (gp 2,3,4) wherea ...19863541363
comparison of five ovine isolates of chlamydia psittaci: an evaluation of three cell culture treatments. 19863543589
serological detection of phage infection in chlamydia psittaci recovered from ducks.antiserum prepared against a phage which infects a chlamydia psittaci isolate recovered from domestic ducks was used to screen other recent avian c psittaci isolates by indirect immunofluorescence. two more phage infected strains from ducks were discovered. however, phage was not detected in every isolate examined from common source ducks, although such birds are likely to be infected with the same c psittaci strain. moreover, phage could not always be demonstrated by indirect immunofluorescence ...19863544465
comparison of some ovine chlamydia psittaci isolates by indirect immunofluorescence.four ovine abortion isolates, including the a22 vaccine isolate, and an isolate from a case of lamb arthritis, were compared by indirect immunofluorescence using antisera raised in chickens and mice. cross titrations with homologous and heterologous antisera showed a one-way reaction between the single chlamydial isolate from a lamb with arthritis and the four isolates from cases of ovine enzootic abortion. the abortion isolates could not be distinguished.19873544468
recognition and treatment of chlamydial infections.the microbiology, laboratory diagnosis, clinical features, and treatment of infectious diseases caused by chlamydia trachomatis and chlamydia psittaci are reviewed. chlamydial genital infection is the most common sexually transmitted disease in the united states. c. trachomatis plays an important role in nongonococcal urethritis, postgonococcal urethritis, cervicitis, pelvic inflammatory disease, and possibly proctitis. proper treatment of genital infections caused by c. trachomatis is important ...19873545650
differences in igg1 and igg2 responses of goats to chlamydial abortions and to clinically inapparent infections detected by the western blot technique. 19863551407
clinical conditions associated with positive complement fixation serology for chlamydiae.the hospital records of 242 patients with diagnostic chlamydial complement fixation (cf) titres (seroconversion and/or titre greater than or equal to 64) found among 60,000 patients screened for suspected viral illnesses were reviewed to study the clinical conditions associated with positive cf serology for chlamydiae. after excluding typical genital c. trachomatis infections, the majority of the remainder were considered to represent c. psittaci infections. respiratory symptoms were the most co ...19873556433
chlamydiosis in psittacine birds. 19873558076
simultaneous vaccination by three living attenuated strains of brucella, salmonella and chlamydia in mice.associations of vaccines for simultaneous administration were successfully developed long ago, in particular in human medicine, although there have been very few attempts to associate living bacterial vaccines. unfortunately, living vaccines can interact with one another resulting in either a virulent infection or an exclusion of one by the other. having developed two new low virulence vaccinal strains of chlamydia psittaci var. ovis and salmonella abortus ovis for use in veterinary medicine, we ...19873577354
experimental infection of sheep with chlamydia psittaci. 19873594193
atypical pneumonia: recognition and treatment.while the term "atypical pneumonia" has been in use for many years, it cannot in fact be defined. however, there is a persuasive reason to retain the clinical use of the term, and that is to provide a guide for the clinician in the choice of empirical antibiotic therapy for patients with acute pneumonia. atypical pneumonia, then, is a descriptive term for a common clinical syndrome. provided certain clinicoepidemiological groups are excluded, the most common infectious causes of this syndrome ar ...19873600471
analysis of the humoral immune response to chlamydial genital infection in guinea pigs.studies using the guinea pig model of chlamydial genital infection with the chlamydia psittaci agent of guinea pig inclusion conjunctivitis (gpic) have shown that serum and local antibodies play a role both in the resolution of infection and in protection against reinfection. thus, this model is suited for further exploration of immune mechanisms and for vaccine studies with chlamydial macromolecules. we have further characterized the model by assessing the antigen-specific antibody response to ...19873610314
unique ultrastructure in the elementary body of chlamydia sp. strain twar.the ultrastructure of two prototype strains (tw-183 and ar-39) of chlamydia sp. strain twar was described. the twar elementary body (eb) demonstrated a unique morphology and structure distinct from those of other chlamydial organisms. it was pleomorphic but typically pear shaped. the average size was 0.38 micron, with a long axis of 0.44 micron, a short axis of 0.31 micron, and a ratio of the long to the short axes of 1.42. the cytoplasmic mass was round, with an average diameter of 0.24 micron. ...19873611029
expression of major histocompatibility complex antigens on macrophages: correlative study using flow cytometry, radioimmunoassay, and colloidal gold immunolabeling.correlative scanning electron microscopy (sem), radioimmunoassay (ria), and flow cytometric analysis were used to characterize levels of class i and class ii major histocompatibility complex-encoded (mhc) antigen expression on peritoneal exudate cells of mice chronically infected with chlamydia psittaci. analysis of peritoneal macrophages by all three techniques revealed a marked induction of h-2 k,d (class i) and i-a, i-e (class ii) antigens on cells from infected c3h mice when compared to unin ...19873616567
[the cat as a possible infection source for chlamydia psittaci keratoconjunctivitis in humans].acute follicular keratoconjunctivitis in a 25-year-old female patient was diagnosed as an infection with chlamydia psittaci supposedly originating from a household cat with similar symptoms. the diagnosis was based on clinical symptoms, the appearance of chlamydia-psittaci-specific igm in the patient and the cat, and excluded other pathogenic agents. chlamydiae organisms could not be isolated, probably due to prior antibiotic treatment. two weeks of continuous treatment led to complete recovery ...19873617045
prophylactic effects of silver nitrate and erythromycin on chlamydia psittaci conjunctivitis.an animal model has been developed to study the effects of various prophylaxis agents on acquisition of chlamydial conjunctivitis. when hartley strain newborn guinea pigs received ocular inoculations of chlamydia psittaci followed by the instillation of various agents, 1% agno3 significantly lowered the risk of developing chlamydial conjunctivitis if it was administered within 15 min after the inoculation with c. psittaci. however, if the agno3 was administered at either 1 hr or 2 hr following i ...19873623840
dominance of chlamydia psittaci-specific igg2 subclass in the humoral immune responses of naturally and experimentally infected cattle.indirect enzyme-linked immunosorbent assays were applied to differentiate chlamydia (c.) psittaci-specific igg1 and igg2 levels in 143 individual serum samples from cattle with naturally occurring chlamydial infections and in 190 sequential serum samples from 26 experimentally infected pregnant cows, calves, and a bull. the mean igg1:igg2 ratio of naturally infected cattle was 1:4 indicating a significant (p less than 0.001) igg2 dominance. similar ratios were detected in the experimentally infe ...19873629940
[severe pneumopathy and acute renal insufficiency disclosing chlamydia psittaci infection: resistance to the treatment with erythromycin].the authors report one case of psittacosis with acute respiratory distress syndrome, acute tubulo-interstitial nephropathy and jaundice. erythromycin therapy was uneffective but recovery was obtained with minocycline and rifampicin treatment. early diagnosis of chlamydia psittaci infection is mandatory in order to prevent severe complications and to institute appropriate antimicrobial therapy.19873631830
nonoxidative antimicrobial effects of human polymorphonuclear leukocyte granule proteins on chlamydia spp. in vitro.proteins from isolated granules of human polymorphonuclear leukocytes were assessed for their nonoxidative microbicidal effect on chlamydiae by two different methods: a radioisotope assay for elementary body integrity and a biological assay for inclusion development. crude granule extract, which consisted of a mixture of all granule proteins, caused a 20 to 30% decrease in infectivity and a 52% decrease in infectivity when incubated with chlamydia psittaci cal-10 and chlamydia trachomatis serova ...19873653985
regulation of chlamydia psittaci (strain guinea pig inclusion conjunctivitis) growth in mccoy cells by amino acid antagonism.chlamydiae have amino acid requirements for growth in tissue culture as defined by those amino acids whose individual omission from the growth medium prevents chlamydial multiplication. we have tested the hypothesis that this inhibition of growth arises as a result of antagonism between particular amino acids such that inhibition occurs when the concentration of one amino acid is reduced in the presence of the antagonist amino acid at high concentration. using the chlamydia psittaci strain guine ...19873655729
analysis of caprine igg1 and igg2 subclass responses to chlamydia psittaci infection and vaccination.enzyme-linked immunosorbent assays (elisas) specific for caprine igg(h+l), igg1 and igg2 were developed and evaluated for serodiagnosis of chlamydia psittaci infections in a tunisian goat flock with currently occurring chlamydial abortions and a clinically inapparent goat flock of an animal research facility. additionally, elisas were applied to record the igg1 and igg2 dynamics of four goats vaccinated with inactivated chlamydia psittaci and coxiella burnetii. for screening purposes, the igg(h+ ...19873660580
[rare forms and aspects of human chlamydia infection]. 19873660881
the low-molecular-mass, cysteine-rich outer membrane protein of chlamydia trachomatis possesses both biovar- and species-specific epitopes.we isolated, by hydroxylapatite high-performance liquid chromatography, 14- and 15-kilodalton (kda), cysteine-rich outer membrane proteins from chlamydia trachomatis tw-5/ot (serovar b) and lgv-434 (serovar l2), respectively. monoclonal antibodies (mabs) were generated against the purified proteins, and their specificities were determined by immunoblotting. mab b-14k recognized an epitope located on the 14-kda cysteine-rich protein of the tw-5/ot strain and was immunoreactive with a comigrating ...19873666953
effect of vaccination on feline chlamydia psittaci infection.experimental ocular infection of specific-pathogen-free cats with the feline pneumonitis strain of chlamydia psittaci produced an acute, severe conjunctivitis characterized by blepharospasm, conjunctival hyperemia, chemosis, and ocular discharge. organisms were recovered from the conjunctiva for several weeks, and persistent genital and gastrointestinal infection also resulted from the ocular infection in some cats. subcutaneous vaccination with live feline pneumonitis c. psittaci 4 weeks before ...19873666957
monoclonal antibody typing of chlamydia psittaci strains derived from avian and mammalian species.a total of 77 chlamydia psittaci strains of avian, human, and mammalian origin were grouped into four serovars with 11 monoclonal antibodies recognizing the lipopolysaccharide and the major outer membrane protein antigens. the avian and human strains, which were closely related to each other, were distinct from the mammalian strains. immunological typing of c. psittaci with monoclonal antibodies seems practical.19873667918
prevalence of chlamydia, toxoplasma, toxocara and ringworm in farm cats in south-west england.the prevalence of infection with chlamydia psittaci, toxoplasma gondii, toxocara cati and microsporum canis was examined in 51 cats on 22 sheep farms in the bristol area. serum antibody to c psittaci and t gondii was present in 45 per cent and 47 per cent of cats, respectively. at the time of sampling c psittaci was isolated from 6 per cent of the cats, t cati was identified in 63 per cent of faecal samples but neither t gondii nor m canis was isolated. when examined according to the farm of ori ...19873672827
harmful aspects of keeping pet birds. 19873681878
survey for antibodies to chlamydia psittaci in cattle in hokkaido. 19873682527
ovine enzootic abortion: experimental studies of immune responses.eight pregnant and four non-pregnant ewes were exposed to experimental oral infection with the chlamydial agent of ovine enzootic abortion. four non-pregnant ewes received a sham inoculum. immune responses were investigated using a complement fixation test (cft), a delayed-type hypersensitivity (dth) skin test and a lymphocyte transformation assay (lta). when these responses were correlated with clinical observations it was concluded that fetopathy occurred in those ewes which failed to mount or ...19863704326
psittacosis in the rsa.patients usually provisionally diagnosed as having typhoid fever or pneumonia are regularly admitted to the rietfontein fever hospital suffering from psittacosis. the main symptoms are intense headache, chills and fever and an irritating non-productive cough. later most patients develop signs of pneumonitis most clearly seen on radiographic examination. an important clue to the diagnosis is a history of contact with birds, most often budgerigars and more recently cockatiels. the diagnosis may be ...19863704861
ovine enzootic abortion: field observations on naturally acquired and vaccine-elicited delayed type hypersensitivity to chlamydia psittaci (ovis). 19863705370
interaction between chlamydia spp. and human polymorphonuclear leukocytes in vitro.chlamydia psittaci and chlamydia trachomatis elementary bodies (eb) incubated in the presence of complement or specific antibody or both caused chemotaxis of human polymorphonuclear leukocytes (pmn) in vitro. reticulate bodies and culture supernatants had no effect on these cells. the ability of chlamydiae to enter and survive in pmn under nonopsonizing conditions was investigated by measuring the association of 3h-labeled eb and of inclusion-forming units with these phagocytes. both assays indi ...19863710578
a new chlamydia psittaci strain, twar, isolated in acute respiratory tract infections.during a 2 1/2-year period, we studied 386 university of washington students with acute respiratory disease, to determine whether a chlamydia psittaci strain, here designated twar, is an important respiratory pathogen. serologic evidence of recent twar infection was found in 13 students, and the organism was isolated from 8 of these. twar infection occurred in 12 percent of the students who had pneumonia (9 of 76), 5 percent of those with bronchitis (3 of 63), and 1 percent of those with pharyng ...19863724806
chlamydia psittaci--"reemergence" of a forgotten pathogen. 19863724807
[diagnostic value of serological and bacteriological methods in sheep affected with s. abortus-ovis and chlamydia psittaci var. ovis infections].it was found that the shedding of salmonella organisms by sheep with salmonella abortions was most active during the first fifteen days following abortions or lambing. in sheep affected with a mixed salmonella abortus ovis and chlamydia psittaci var. ovis infection this period was longer (about 30 days). the excretion of salmonella organisms was more strongly manifested with the vaginal discharge than with the feces, urine, and milk. no correlation was established between the titer value of the ...19863727383
chlamydia psittaci latex agglutination antigen for rapid detection of antibody activity in avian sera: comparison with direct complement fixation and isolation results.cell-culture-grown chlamydia psittaci of turkey origin was treated with phenol and partially purified by differential centrifugation. the most stable antigen/latex mixture occurred with crude antigen precipitated by dimethyl sulfoxide and digested with trypsin. agglutination reactions occurred within 2 minutes when antigen/latex and antibody-positive serum mixtures were rotated to facilitate contact of the reagents. nonspecific agglutination of control latex occurred with 1.2% of clinical sera. ...19863729870
[hepatic manifestations of psittacosis].a moderate increase in transaminases and alkaline phosphatase serum concentrations was observed in 14 of 32 patients with psittacosis (antibodies anti-chlamydia psittaci greater than or equal to 1/40 with the complement fixation test). clinical manifestations were present in 4 patients (hepatomegaly in 4 cases; jaundice in one case). in one patient, histological examination of a liver specimen showed granuloma. in 13 patients treated with tetracycline or macrolides, the outcome was favorable. on ...19863732734
chlamydiosis in birds in great britain. 1. serological reactions to chlamydia in birds sampled between 1974 and 1983.a total of 6593 serum samples from birds, received for diagnostic testing or surveys between 1974 and 1983, were titrated by direct complement fixation (cf) test against chlamydia psittaci antigen. the percentage of positive reactions found was variable for domestic poultry, ranging from 1.5 in chickens to 22.2 in geese, and was highest in pigeons columba livia (47.3) and collared doves streptopelia decaocto (51.4). a moderate incidence was found for game birds (29.0), wild ducks (23.3) and impo ...19863734430
chlamydiosis in birds in great britain. 2. isolations of chlamydia psittaci from birds sampled between 1976 and 1984.a total of 1531 diagnostic submissions from birds were examined by culture for the presence of chlamydia psittaci between june 1976 and december 1984 by growth in nctc 929 clone l mouse fibroblast cells, pretreated with an inhibitor of cell division, followed by direct immunofluorescence microscopy. of these, 196 were found positive. the continued importance of psittacine birds as potential sources of infection was shown by the high number of positives (139) obtained from birds of that order. th ...19863734431
rapid detection of chlamydia psittaci in vaginal swabs of aborted ewes and goats by enzyme linked immunosorbent assay (elisa).an enzyme linked immunosorbent assay (elisa) for the detection of chlamydia psittaci in vaginal swabs of aborted ewes and goats has been developed using microtiter plates coated with sheep anti-chlamydia immunoglobulin g. this technique was compared to the direct isolation of the agent by plaque assay on mccoy cells. among 89 specimens from animals in infected flocks, 58 were positive by both methods, seven were only positive by elisa, and nine others were only positive by direct isolation (plaq ...19863739210
distinguishing between ovine abortion and ovine arthritis chlamydia psittaci isolates with specific monoclonal antibodies.monoclonal antibodies to chlamydia psittaci were prepared by both in vivo and in vitro immunization methods, using an abortion strain of c psittaci as the immunizing antigen. seven of the 8 monoclonal antibodies produced were genus-specific by the enzyme-linked immunosorbent assay and immunofluorescence test. the genus-specific antibodies were reactive with a protease-resistant, periodate-sensitive antigen of less than 14 kilodaltons. the remaining monoclonal antibody, 10d7, was specific for ovi ...19863740621
potential subtypes of chlamydia psittaci based on effects of cortisone with cytochalasin b or cycloheximide.strains of chlamydia psittaci were differentiated as to their biological characteristics using growth in vero cells. these host cells were chlamydial infected without centrifugation, but treated with either cortisone and cytochalasin b or cortisone and cycloheximide. the criterion was the time of 100% cytopathic effect and specific sloughing, accompanied by the maximum infectivity of c. psittaci. possible subtypic characteristics were determined for three avian strains.19863742349
fate of chlamydia trachomatis in human monocytes and monocyte-derived macrophages.the fate of chlamydia trachomatis (l2/434/bu) in human peripheral blood monocytes and human monocyte-derived macrophages was studied by transmission electron microscopy (tem) and by measuring the yield of infectious c. trachomatis in one-step growth experiments. two main types of phagosome were seen by tem in the cytoplasm of c. trachomatis-infected human monocytes (1 h postinfection [p.i.]): one in which the elementary body (eb) was tightly surrounded by the membrane of the phagosome and anothe ...19863759241
intracerebral infection of mice with ovine strains of chlamydia psittaci: an animal screening test for the assay of vaccines.intracerebral inoculation of mice with the a22 strain of ovine c. psittaci gave a reproducible non-lethal infection; multiplication of the inoculum could be quantitated by titration of mouse brain extracts in tissue culture. mice which had recovered from infection, or which had been inoculated subcutaneously with living organisms of a22 strain, showed solid resistance to intracerebral challenge infection. however, subcutaneous inoculation of formalin-inactivated chlamydia showed little protectiv ...19863760263
[ornithosis as a general systemic disorder]. 19863762748
[immune response in ornithosis and antibiotic therapy]. 19863764553
wild-pigeon-related psittacosis in a family.three members of a family acquired psittacosis after exposure to a wild pigeon. each of the patients had pulmonary infiltrates, prominent headache, abdominal complaints, and serologic evidence for infection with chlamydia psittaci. of 759 cases of psittacosis reported to the centers for disease control for the period of 1974 to 1981, some 75 (10 percent) were associated with pigeons. fifty-two of the cases were associated with domestic pigeons and 23 with wild pigeons. pigeons represent a largel ...19863769573
chemical, biological, and immunochemical properties of the chlamydia psittaci lipopolysaccharide.the lipopolysaccharide (lps) of chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. the lps contained d-galactosamine, d-glucosamine, phosphorus, long-chain fatty acids, and 3-deoxy-d-manno-2-octulosonic acid in the molar ratio of approximately 1:2:2:6:5. the antigenic properties of the isolated lps were compared with those of the lps from chlamydia trachomatis and salmonella minnesota re by the passi ...19863770953
isolation of chlamydia psittaci from the respiratory tract and conjunctivae of thoroughbred horses. 19863776033
comparison of the virulence in mice of some ovine isolates of chlamydia psittaci.virulence associated with five ovine isolates of chlamydia psittaci was compared using a mouse model. comparable doses of each isolate were inoculated via the footpad into separate groups of 6-week-old mice. four isolates from abortion were judged more virulent than an isolate from arthritis because of their ability to migrate from the site of infection to the spleen. similar results were obtained using three different mouse strains. re-isolation of c. psittaci indicated variation in susceptibil ...19863776092
isolation of chlamydia psittaci from an aborted bovine foetus. 19863778376
evaluation of a monoclonal antibody based elisa for detection of feline chlamydia psittaci.a commercially available elisa designed for the detection of c trachomatis in human urogenital specimens was compared with cell culture for the detection of chlamydia psittaci in cat conjunctival swabs and in twofold dilutions of a cell culture pool of a feline strain of c psittaci. cell culture was more sensitive than the elisa test for detection of c psittaci.19863788002
[a method for the determination of antibiotic sensitivity of chlamydias in vitro]. 19863788356
subacute thyroiditis associated with chlamydia psittaci infection. 19863797361
chlamydia psittaci: inclusion morphology in cell culture and virulence in mice of ovine isolates. 19863798694
response of goats to vaccination with temperature-sensitive mutants of chlamydia psittaci obtained by nitrosoguanidine mutagenesis.two temperature-sensitive strains of ovine chlamydia psittaci, 1b and 1h, obtained by mutagenesis were used as live-organism vaccines; 31 goats were given 4 x 10(6) plaque-forming units (pfu) of strain 1b, and 31 were given 5 x 10(6) pfu of strain 1h 2 months before they were bred. the consequences of the vaccination of the goats were studied during pregnancy by recording complement-fixation antibody titer, chlamydial excretion, and kidding performances and were compared with those of goats inoc ...19863800123
successful treatment by doxycycline of endocarditis caused by ornithosis.the case of a 59 year old man with ornithosis endocarditis and a history of contact with pheasants is reported. treatment with oxytetracycline was not tolerated and so doxycycline was substituted. over the two year follow up the patient's clinical condition and serial echocardiographic appearances improved and chlamydia psittaci complement fixation titres returned to normal. valve replacement was not required.19873801260
[intensity of the epizootic process in sheep infected with s. abortus ovis and chlamydia psittaci var. ovis].an attempt was made to analyze comparatively the intensity of the epizootic process in young female sheep, ewes (second pregnancy, with no records of abortions in their first pregnancy) and their lambs either with an infection of salmonella abortus ovis only or with a mixed infection of s. abortus ovis and chlamydia psittaci var. ovis. this was reached through following up and studying the parameters morbidity, mortality, lethality, index of infectedness, index of deadlines, and fertility. it wa ...19863811213
[field study on the development of resistance of chlamydia psittaci to tetracyclines]. 19863814070
[ocular findings in chlamydia psittaci-induced keratoconjunctivitis in the human]. 19863817682
diagnosis of pneumococcal pneumonia by enzyme-linked immunosorbent assay of antibodies to pneumococcal hemolysin (pneumolysin).an enzyme-linked immunosorbent assay (elisa) with a highly purified pneumolysin as the antigen was evaluated for serological diagnosis of pneumococcal pneumonia. one hundred four healthy controls were tested, and the specificity of the test was set to 95%. in samples from patients with bacteremic pneumococcal pneumonia, 82% (18 of 22) were positive, i.e., at least one serum sample had a titer above the upper normal limit or at least a twofold rise in antibody titers was noted. in nonbacteremic p ...19873818919
the spectrum of pneumonia in 1983 at the riyadh armed forces hospital.the agents causing pneumonia have been assessed in 112 adult patients admitted to the armed forces hospital in riyadh during a period of one year: pathogens were identified in 78 patients (69.6%). sputum culture produced a significant isolate in 60 patients (53.5%), and in 17 (15.2%) the causative agent was suggested by serological tests. streptococcus pneumonia was the commonest infecting agent (21.4%). pneumonia due to mycobacterium tuberculosis was diagnosed in eight patients, to mycoplasma p ...19873819456
[chlamydia infections in switzerland. comparative study of diagnostic technics]. 19873823827
atypical community-acquired pneumonia: concurrent infection with chlamydia psittaci and mycobacterium tuberculosis.we have presented a case of combined pulmonary infection with chlamydia psittaci and mycobacterium tuberculosis, both documented by culture of lymph nodes and respiratory secretions. we describe it not only as an unusual occurrence, but also to illustrate the importance of a systematic and thorough diagnostic approach to every case of pneumonia.19873824037
isolation of chlamydia psittaci from commercial broiler chickens. 19863827778
influence of cysteine deprivation on chlamydial differentiation from reproductive to infective life-cycle forms.the effects of omission of individual amino acids from growth medium on the differentiation of chlamydia trachomatis dk-20 (serotype e) during infection of cycloheximide-treated mccoy cells are described. as judged by inclusion body staining with acridine orange, omission of cysteine from the medium severely retarded differentiation of reproductive reticulate body (rb) to infective elementary body (eb) forms. the effect appeared specific to cysteine in that omission of other amino acids had litt ...19853831232
chlamydia isolated from abortion in sheep. 19853833212
chlamydial endocarditis.echocardiographic diagnosis of a case of endocarditis, subsequently proven to be due to a chlamydial organism, is described. early echocardiographic diagnosis guided initial medical management and directed urgent surgical intervention when the patient deteriorated. reported cases of chlamydial endocarditis are reviewed.19853864426
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