| survey for antibodies to chlamydia psittaci in cattle in hokkaido. | | 1987 | 3682527 |
| ovine enzootic abortion: experimental studies of immune responses. | eight pregnant and four non-pregnant ewes were exposed to experimental oral infection with the chlamydial agent of ovine enzootic abortion. four non-pregnant ewes received a sham inoculum. immune responses were investigated using a complement fixation test (cft), a delayed-type hypersensitivity (dth) skin test and a lymphocyte transformation assay (lta). when these responses were correlated with clinical observations it was concluded that fetopathy occurred in those ewes which failed to mount or ... | 1986 | 3704326 |
| psittacosis in the rsa. | patients usually provisionally diagnosed as having typhoid fever or pneumonia are regularly admitted to the rietfontein fever hospital suffering from psittacosis. the main symptoms are intense headache, chills and fever and an irritating non-productive cough. later most patients develop signs of pneumonitis most clearly seen on radiographic examination. an important clue to the diagnosis is a history of contact with birds, most often budgerigars and more recently cockatiels. the diagnosis may be ... | 1986 | 3704861 |
| ovine enzootic abortion: field observations on naturally acquired and vaccine-elicited delayed type hypersensitivity to chlamydia psittaci (ovis). | | 1986 | 3705370 |
| interaction between chlamydia spp. and human polymorphonuclear leukocytes in vitro. | chlamydia psittaci and chlamydia trachomatis elementary bodies (eb) incubated in the presence of complement or specific antibody or both caused chemotaxis of human polymorphonuclear leukocytes (pmn) in vitro. reticulate bodies and culture supernatants had no effect on these cells. the ability of chlamydiae to enter and survive in pmn under nonopsonizing conditions was investigated by measuring the association of 3h-labeled eb and of inclusion-forming units with these phagocytes. both assays indi ... | 1986 | 3710578 |
| a new chlamydia psittaci strain, twar, isolated in acute respiratory tract infections. | during a 2 1/2-year period, we studied 386 university of washington students with acute respiratory disease, to determine whether a chlamydia psittaci strain, here designated twar, is an important respiratory pathogen. serologic evidence of recent twar infection was found in 13 students, and the organism was isolated from 8 of these. twar infection occurred in 12 percent of the students who had pneumonia (9 of 76), 5 percent of those with bronchitis (3 of 63), and 1 percent of those with pharyng ... | 1986 | 3724806 |
| [diagnostic value of serological and bacteriological methods in sheep affected with s. abortus-ovis and chlamydia psittaci var. ovis infections]. | it was found that the shedding of salmonella organisms by sheep with salmonella abortions was most active during the first fifteen days following abortions or lambing. in sheep affected with a mixed salmonella abortus ovis and chlamydia psittaci var. ovis infection this period was longer (about 30 days). the excretion of salmonella organisms was more strongly manifested with the vaginal discharge than with the feces, urine, and milk. no correlation was established between the titer value of the ... | 1986 | 3727383 |
| chlamydia psittaci latex agglutination antigen for rapid detection of antibody activity in avian sera: comparison with direct complement fixation and isolation results. | cell-culture-grown chlamydia psittaci of turkey origin was treated with phenol and partially purified by differential centrifugation. the most stable antigen/latex mixture occurred with crude antigen precipitated by dimethyl sulfoxide and digested with trypsin. agglutination reactions occurred within 2 minutes when antigen/latex and antibody-positive serum mixtures were rotated to facilitate contact of the reagents. nonspecific agglutination of control latex occurred with 1.2% of clinical sera. ... | 1986 | 3729870 |
| chlamydiosis in birds in great britain. 2. isolations of chlamydia psittaci from birds sampled between 1976 and 1984. | a total of 1531 diagnostic submissions from birds were examined by culture for the presence of chlamydia psittaci between june 1976 and december 1984 by growth in nctc 929 clone l mouse fibroblast cells, pretreated with an inhibitor of cell division, followed by direct immunofluorescence microscopy. of these, 196 were found positive. the continued importance of psittacine birds as potential sources of infection was shown by the high number of positives (139) obtained from birds of that order. th ... | 1986 | 3734431 |
| rapid detection of chlamydia psittaci in vaginal swabs of aborted ewes and goats by enzyme linked immunosorbent assay (elisa). | an enzyme linked immunosorbent assay (elisa) for the detection of chlamydia psittaci in vaginal swabs of aborted ewes and goats has been developed using microtiter plates coated with sheep anti-chlamydia immunoglobulin g. this technique was compared to the direct isolation of the agent by plaque assay on mccoy cells. among 89 specimens from animals in infected flocks, 58 were positive by both methods, seven were only positive by elisa, and nine others were only positive by direct isolation (plaq ... | 1986 | 3739210 |
| distinguishing between ovine abortion and ovine arthritis chlamydia psittaci isolates with specific monoclonal antibodies. | monoclonal antibodies to chlamydia psittaci were prepared by both in vivo and in vitro immunization methods, using an abortion strain of c psittaci as the immunizing antigen. seven of the 8 monoclonal antibodies produced were genus-specific by the enzyme-linked immunosorbent assay and immunofluorescence test. the genus-specific antibodies were reactive with a protease-resistant, periodate-sensitive antigen of less than 14 kilodaltons. the remaining monoclonal antibody, 10d7, was specific for ovi ... | 1986 | 3740621 |
| potential subtypes of chlamydia psittaci based on effects of cortisone with cytochalasin b or cycloheximide. | strains of chlamydia psittaci were differentiated as to their biological characteristics using growth in vero cells. these host cells were chlamydial infected without centrifugation, but treated with either cortisone and cytochalasin b or cortisone and cycloheximide. the criterion was the time of 100% cytopathic effect and specific sloughing, accompanied by the maximum infectivity of c. psittaci. possible subtypic characteristics were determined for three avian strains. | 1986 | 3742349 |
| fate of chlamydia trachomatis in human monocytes and monocyte-derived macrophages. | the fate of chlamydia trachomatis (l2/434/bu) in human peripheral blood monocytes and human monocyte-derived macrophages was studied by transmission electron microscopy (tem) and by measuring the yield of infectious c. trachomatis in one-step growth experiments. two main types of phagosome were seen by tem in the cytoplasm of c. trachomatis-infected human monocytes (1 h postinfection [p.i.]): one in which the elementary body (eb) was tightly surrounded by the membrane of the phagosome and anothe ... | 1986 | 3759241 |
| intracerebral infection of mice with ovine strains of chlamydia psittaci: an animal screening test for the assay of vaccines. | intracerebral inoculation of mice with the a22 strain of ovine c. psittaci gave a reproducible non-lethal infection; multiplication of the inoculum could be quantitated by titration of mouse brain extracts in tissue culture. mice which had recovered from infection, or which had been inoculated subcutaneously with living organisms of a22 strain, showed solid resistance to intracerebral challenge infection. however, subcutaneous inoculation of formalin-inactivated chlamydia showed little protectiv ... | 1986 | 3760263 |
| [ornithosis as a general systemic disorder]. | | 1986 | 3762748 |
| [immune response in ornithosis and antibiotic therapy]. | | 1986 | 3764553 |
| chemical, biological, and immunochemical properties of the chlamydia psittaci lipopolysaccharide. | the lipopolysaccharide (lps) of chlamydia psittaci was extracted from yolk sac-grown elementary bodies, purified, and characterized chemically, immunochemically, and biologically. the lps contained d-galactosamine, d-glucosamine, phosphorus, long-chain fatty acids, and 3-deoxy-d-manno-2-octulosonic acid in the molar ratio of approximately 1:2:2:6:5. the antigenic properties of the isolated lps were compared with those of the lps from chlamydia trachomatis and salmonella minnesota re by the passi ... | 1986 | 3770953 |
| isolation of chlamydia psittaci from the respiratory tract and conjunctivae of thoroughbred horses. | | 1986 | 3776033 |
| comparison of the virulence in mice of some ovine isolates of chlamydia psittaci. | virulence associated with five ovine isolates of chlamydia psittaci was compared using a mouse model. comparable doses of each isolate were inoculated via the footpad into separate groups of 6-week-old mice. four isolates from abortion were judged more virulent than an isolate from arthritis because of their ability to migrate from the site of infection to the spleen. similar results were obtained using three different mouse strains. re-isolation of c. psittaci indicated variation in susceptibil ... | 1986 | 3776092 |
| isolation of chlamydia psittaci from an aborted bovine foetus. | | 1986 | 3778376 |
| [a method for the determination of antibiotic sensitivity of chlamydias in vitro]. | | 1986 | 3788356 |
| chlamydia psittaci: inclusion morphology in cell culture and virulence in mice of ovine isolates. | | 1986 | 3798694 |
| response of goats to vaccination with temperature-sensitive mutants of chlamydia psittaci obtained by nitrosoguanidine mutagenesis. | two temperature-sensitive strains of ovine chlamydia psittaci, 1b and 1h, obtained by mutagenesis were used as live-organism vaccines; 31 goats were given 4 x 10(6) plaque-forming units (pfu) of strain 1b, and 31 were given 5 x 10(6) pfu of strain 1h 2 months before they were bred. the consequences of the vaccination of the goats were studied during pregnancy by recording complement-fixation antibody titer, chlamydial excretion, and kidding performances and were compared with those of goats inoc ... | 1986 | 3800123 |
| [field study on the development of resistance of chlamydia psittaci to tetracyclines]. | | 1986 | 3814070 |
| [chlamydia infections in switzerland. comparative study of diagnostic technics]. | | 1987 | 3823827 |
| isolation of chlamydia psittaci from commercial broiler chickens. | | 1986 | 3827778 |
| influence of cysteine deprivation on chlamydial differentiation from reproductive to infective life-cycle forms. | the effects of omission of individual amino acids from growth medium on the differentiation of chlamydia trachomatis dk-20 (serotype e) during infection of cycloheximide-treated mccoy cells are described. as judged by inclusion body staining with acridine orange, omission of cysteine from the medium severely retarded differentiation of reproductive reticulate body (rb) to infective elementary body (eb) forms. the effect appeared specific to cysteine in that omission of other amino acids had litt ... | 1985 | 3831232 |
| chlamydia isolated from abortion in sheep. | | 1985 | 3833212 |
| chlamydial endocarditis. | echocardiographic diagnosis of a case of endocarditis, subsequently proven to be due to a chlamydial organism, is described. early echocardiographic diagnosis guided initial medical management and directed urgent surgical intervention when the patient deteriorated. reported cases of chlamydial endocarditis are reviewed. | 1985 | 3864426 |
| duck hepatitis virus and chlamydia psittaci outbreak. | | 1985 | 3872526 |
| in vitro expression of factor-mediated cytotoxic activity generated during the immune response to chlamydia in the mouse. | supernatant fluid (sf) prepared by mitogen incubation of spleen cells from a/j mice previously immunized against lethal challenge by the 6bc strain of chlamydia psittaci was cytotoxic for mouse fibroblasts (l cells) infected with 6bc, as detected by the [3h]thymidine release assay and the trypan blue exclusion test. in contrast, sf prepared from spleen cells taken from unimmunized animals (controls) was not cytotoxic when added to infected l cells. no cytotoxicity was observed when sf was added ... | 1985 | 3872911 |
| comparative titers of egg assay against immunofluorescent assay of chlamydia psittaci. | a comparison of titers was made between an egg assay and a direct fluorescent antibody assay of three chlamydial strains propagated in vero cells with and without cortisone plus cytochalasin b. the titer of nj-1 strain was similar in the egg titration and the fluorescent antibody assay in the untreated sample and a little lower for the sample treated with cytochalasin b and cortisone. the sct and cdc strains had approximately the same titers in the egg titration and the fluorescent antibody assa ... | 1985 | 3886106 |
| an epidemic of mild pneumonia due to an unusual strain of chlamydia psittaci. | an epidemic of mild pneumonia was discovered during a chest radiographic survey of adolescents and young adults in two communities 110 kilometers apart in northern finland. antibodies to chlamydial antigens were found in 32 of 34 persons with pneumonitis. microimmunofluorescence antibody tests suggest that the etiologic agent is closely related or identical to tw-183, an unusual strain of chlamydia psittaci isolated from the eye of a child in taiwan. the point prevalence of pneumonitis with anti ... | 1985 | 3886806 |
| direct immunoperoxidase method for demonstrating chlamydia psittaci in tissue sections. | | 1985 | 3891793 |
| [use of the immunofluorescent microagglutination reaction for the intrageneric differentiation of the immune response in chlamydiosis]. | the possibility of using, on principle, the immunofluorescent microagglutination test with chlamydia fluorescent corpuscular antigen for the strain-specific differentiation of immune response induced by the causative agents of ornithosis (strain b), enzootic abortus ovis (strain eae) and lymphogranuloma venereum (strain lv) has been experimentally demonstrated. the calculation of indices, characterizing the specificity of differences between the systems under comparison, by the method of s. fras ... | 1985 | 3892987 |
| new view of the surface projections of chlamydia trachomatis. | two kinds of surface specializations of chlamydiae have been described: hemispheric projections and spikelike rods. we undertook the present studies to demonstrate chlamydial ultrastructure in greater detail in conventional thin-sectioned specimens. chlamydia trachomatis (lgv strain l2/434/bu), cultured for 40 h in l929 mouse fibroblasts, was fixed in glutaraldehyde-acrolein, p-formaldehyde-glutaraldehyde, or glutaraldehyde-osmium tetroxide mixtures, postfixed in osmium tetroxide, stained in ura ... | 1985 | 3900041 |
| antigenic diversity of chlamydia psittaci of mammalian origin determined by microimmunofluorescence. | a group of twenty-five isolates of chlamydia psittaci representing at least seven different biotypes of bovine, ovine, caprine, equine, feline, porcine, and guinea pig origin were immunotyped by an indirect microimmunofluorescence test. different groups of chlamydia-free balb/c mice received two weekly intravenous inoculations with chicken embryo-propagated, partially purified elementary bodies of each strain. antisera for immunotyping were collected 4 days after the first inoculation and 3 to 4 ... | 1985 | 3905618 |
| [chlamydia infections in internal medicine]. | | 1985 | 3910859 |
| properties of monoclonal antibodies to the genus-specific antigen of chlamydia and their use for antigen detection by reverse passive haemagglutination. | the chlamydial genus-specific antigen was extracted with phenol/chloroform/petroleum ether (pcp) from preparations of chlamydia trachomatis and c. psittaci, and quantities measured using an assay for lipopolysaccharide (lps). the lps from c. trachomatis contained 2.2% (w/w) of ketodeoxyoctanoic acid. five igg monoclonal antibodies reacted in an elisa with lps from both species, the antigen being periodate-sensitive and heat-resistant, confirming that all antibodies were against the genus-specifi ... | 1985 | 3921656 |
| molecular characterization of chlamydia trachomatis and chlamydia psittaci plasmids. | plasmids from chlamydia trachomatis lgv-434 (serotype l2) and chlamydia psittaci meningopneumonitis strain cal-10 were cloned into the bamhi and ecori sites of pbr322, respectively. the recombinant plasmids pctl2 and pcpmn, each containing an entire respective chlamydial plasmid, were transformed into escherichia coli. the sizes of the plasmids of c. trachomatis and c. psittaci were 7.3 and 6.2 kilobases, respectively. the two plasmids were found to be distinct by restriction endonuclease analys ... | 1986 | 3943908 |
| synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of chlamydia psittaci and chlamydia trachomatis. | the disulfide bond cross-linked major outer membrane protein (momp) of the extracellular elementary bodies (ebs) of chlamydia psittaci was reduced to its monomeric form within 1 h of entry of ebs into host cells by a process which was inhibited by chloramphenicol, while monomeric forms of three cross-linked cysteine-rich proteins could not be detected in sarkosyl outer membrane complexes at any time in either extracellular or intracellular forms of c. psittaci. synthesis and incorporation of the ... | 1986 | 3944054 |
| respiratory disease (rhinotracheitis) in turkeys in brittany, france, 1981-1982. ii. laboratory findings. | after discovering that numerous turkey flocks experiencing rhinotracheitis in brittany, france, had antibodies against chlamydia, laboratory studies were conducted to determine whether chlamydia and/or viruses would explain the respiratory disease observed. although both lentogenic paramyxoviruses of type 1 (newcastle disease virus) and chlamydia psittaci were isolated, it was concluded, based on epidemiologic and other laboratory findings, that c. psittaci was the primary cause of the disease. | 1985 | 3985882 |
| [role of eye manifestations in chlamydioses]. | following an up-to-date bacteriological resume, are recalled the most important clinical manifestations presently attributed to c. trachomatis: general manifestations (pulmonary, articular, venereal, congenital, viscereal and others still under discussion); ocular manifestations (trachoma, inclusion conjunctivitis of newborn infants or adults, other ocular affections still under discussion). the main theories are exposed, which try to explain some epidemiological links between all these manifest ... | 1985 | 3990540 |
| enigmatic psittacine chlamydiosis: results of serotesting and isolation attempts, 1978 through 1983, and considerations for the future. | from 1978 through 1983, chlamydiosis was diagnosed by isolation of chlamydia psittaci from various types of psittacine birds. the organism was isolated from 126 (30.4%) of 414 tissue specimens, with the percentages ranging from 12.5% (budgerigars) to 42.8% (cockatiels), excluding 2 parakeets with 1 isolation (50%). from 1,035 cloacal swab/feces specimens, 51 (4.9%) isolations were made, ranging from 1.4% from african grays (1 of 70) to 27.8% from lovebirds (5 of 18). positive direct microscopic ... | 1985 | 3997621 |
| synthesis of protein in host-free reticulate bodies of chlamydia psittaci and chlamydia trachomatis. | synthesis of protein by the obligate intracellular parasitic bacteria chlamydia psittaci (6bc) and chlamydia trachomatis (serovar l2) isolated from host cells (host-free chlamydiae) was demonstrated for the first time. incorporation of [35s]methionine and [35s]cysteine into trichloroacetic acid-precipitable material by reticulate bodies of chlamydiae persisted for 2 h and was dependent upon a exogenous source of atp, an atp-regenerating system, and potassium or sodium ions. magnesium ions and am ... | 1985 | 3997784 |
| rickettsiae in the sumava region. | antibodies were detected to c oxiella burnetii, rickettsia slovaca and chlamydia psittaci in the blood of small terrestrial mammals, larger wild and domestic animals and humans in the southern part of the protected landscape area of the sumava region between 1977 and 1982. the data obtained have contributed to the safe development of livestock raising in this region. | 1985 | 4007702 |
| [generalities on chlamydia. clinical, diagnostic and therapeutic applications]. | | 1985 | 4008874 |
| 'going-light' and psittacosis. | | 1985 | 4013038 |
| attempted venereal transmission of chlamydia psittaci in sheep. | attempts were made to transmit chlamydia psittaci, the causal agent of enzootic abortion of ewes, in three different ways. ten ewes were inseminated artificially with freshly collected semen containing 10(5) celd50 chlamydia. serological evidence of infection was found two weeks before parturition in nine ewes and the organism was recovered from three of them. by six weeks post partum antibody titres had fallen and were negative in six ewes. twenty-four hours after service two groups of 10 ewes ... | 1985 | 4013041 |
| etiology of community-acquired pneumonia in patients requiring hospitalization. | the etiology of community-acquired pneumonia was studied in 127 patients with roentgenologically verified pneumonia who needed hospitalization. etiology was determined on the basis of a positive blood culture and/or a significant antibody titer increase. streptococcus pneumoniae was the probable etiological agent in 69 patients, nontypeable haemophilus influenzae in five patients, streptococcus pyogenes in two patients, and legionella pneumophila and staphylococcus aureus in one patient each. ev ... | 1985 | 4018066 |
| assessing the number of genital chlamydial infections in the united states. | the prevalence and incidence of genital infections with chlamydia trachomatis have not yet been determined for broadly based populations within the united states. largely on the basis of results of prevalence studies from sexually transmitted disease clinics, it has been estimated that c. trachomatis infections are the most common of all sexually transmitted infections and may number 3-5 million cases annually. c. trachomatis isolation rates range from 50% to 80% in certain diseases known to be ... | 1985 | 4020783 |
| [detection of chlamydia in patients with trachoma]. | | 1985 | 4028211 |
| electron microscopic observations concerning the in vivo uptake and release of the agent of guinea-pig inclusion conjunctivitis (chlamydia psittaci) in guinea-pig exocervix. | this report details electron-microscopical observations concerning c. psittaci infection in vivo. the model employed was that of the guinea-pig infected at the exocervical region with the agent of guinea-pig inclusion conjunctivitis (gpic). our observations indicate that chlamydial particles gain access to their target cells by the mechanism of endocytosis. single gpic elementary bodies were seen to be positioned within individual endosomes. the observations reported here provide evidence that c ... | 1985 | 4031129 |
| [use of a concentrated and purified vaccine against chlamydial abortion in sheep]. | attempts were made to immunize sheep against chlamydial abortion with a purified, concentrated, and absorbed vaccine. the latter proved innocuous and unreactive, and was applied at rates of 0.5 to 1.00 cm3. it was found that at a single vaccination of the sheep with 0.5 cm3 as many as 91.42 per cent responded with the production of antichlamydial complement-fixing antibodies on the 45th day of 1:8 to 1:512 titers; 96.43 per cent of the animals responded on the 111th day, the titers ranging from ... | 1985 | 4035984 |
| establishment of hybridomas secreting monoclonal antibodies to chlamydia psittaci. | eleven stable anti-chlamydia hybrid clones by fusions between x63-ag8653 myelomas and immune splenocytes from chlamydia psittaci immunized f1 (c57bl6 x balb/c) mice have been established which react with the 12 reference chlamydia strains (seven c. trachomatis and five c. psittaci. ten of these monoclonal antibodies are directed against the genera-specific epitope (40,000 mw component) for which prolonged immunization seems to be responsible. | 1985 | 4044019 |
| antibodies against chlamydia measured by an elisa method. | an enzyme-linked immunosorbent assay (elisa) for the detection of serum igg and igm antibodies against chlamydia group antigen is presented. 187 selected sera were tested both in the elisa and in the complement-fixation test (cft). of 71 sera with negative results in the cft, 19 (26.8%) were positive in the elisa. of 116 cft positive sera, ten (8,6%) were elisa negative. in 35 of 72 sera (48.6%) with cft titre less than 40 chlamydial igm was demonstrated, while 11 of 115 sera (9.5%) with cft tit ... | 1985 | 4050443 |
| [chlamydia infection during pregnancy]. | | 1985 | 4054008 |
| persistent infection of l cells with an ovine abortion strain of chlamydia psittaci. | l cells inoculated at multiplicities of infection greater than or equal to 1 inclusion-forming unit of the abortigenic chlamydial strain b577 were destroyed within 10 to 15 days. upon continued incubation in fresh medium, a few surviving cells repopulated the flasks, and the reemerging cultures remained persistently infected. the persistent state was characterized by cycles of repopulation with a low ratio of infected cells and cycles of extensive cytopathic changes in which greater than 90% of ... | 1985 | 4055027 |
| role of cell-mediated immunity in chlamydial infection: implications for ocular immunity. | cellular immunity to chlamydia trachomatis is not well understood. studies with the mouse pneumonitis agent in a mouse pneumonia model suggest a strong thymic component to immunity and a role for the macrophage as an effector cell in cell-mediated immunity to c. trachomatis. experiments with chlamydia psittaci indicate that cellular cytotoxic mechanisms and biostatic or cytotoxic mechanisms involving cytokines may also play a role in host defense against chlamydiae. interferon has been shown to ... | 1985 | 4070909 |
| direct complement fixation and isolation attempts for detecting chlamydia psittaci infection of psittacine birds. | serum and fecal specimens from 147 birds were tested for chlamydia psittaci. isolation rates ranged from 3.4% of 88 birds that had titers less than 8, to 50% of six birds that had titers greater than or equal to 128. testing of 44 paired sera revealed a significant titer increase in 5 pairs, a significant titer decrease in 8 pairs, and a stable titer in 31 pairs. the examination of both feces and serum is recommended for determining chlamydia psittaci infections in individual live psittacine bir ... | 1985 | 4074254 |
| [pulmonary infections caused by chlamydia psittaci. clinical and epidemiological study of 31 sporadic cases attended at a general hospital]. | | 1985 | 4079494 |
| seroepidemiological surveillance of chlamydia psittaci in cats and dogs in japan. | | 1985 | 4082403 |
| [chlamydial infection in pericarditis in pigs]. | an etiologic study was carried out for the presence of chlamydia in the case of pericarditis in pigs on a large swine-breeding complex. investigated were some clinical, epizootiologic, and morphologic aspects of the disease. a strain of chlamydia psittaci was isolated in chick embryos from pericardial liquids of affected animals. it was identified through electron-microscopic and antigenic studies. elementary and initial bodies of chlamydia were seen in ultrathin sections of the infected yolk sa ... | 1985 | 4082488 |
| [course of chlamydia-induced inclusion conjunctivitis in the guinea pig in experimental animal husbandry]. | | 1985 | 4082800 |
| [chlamydial infection with main symptoms of upper cervical lymphadenitis (ucla). isolation of chlamydiae]. | | 1985 | 4087073 |
| an enzyme-linked immunosorbent assay (elisa) for the detection of chlamydial antibodies in caprine sera. | chlamydia psittaci is one of the major cause of abortion in goats. the complement-fixation (cf) test is widely used for detection of chlamydial antibodies in caprine sera. a simple, rapid enzyme-linked immunosorbent assay (elisa) has been developed for the measurement of immunoglobulins g (igg). the chlamydia psittaci strain ab3 grown in yolk sac was used as antigen. elisa proved satisfactory for the detection of serum igg; a total of 204 sera were tested and the results correlated well with tho ... | 1985 | 4091495 |
| acquired valvular heart-disease in patients who keep pet birds. | | 1974 | 4143008 |
| [ornithosis and influenza with multiple titer rises in complement-fixation (author's transl)]. | | 1974 | 4157072 |
| [particle membranes of the causative agent of ornithosis]. | | 1974 | 4439878 |
| [morphological manifestations of the intracellular toxic action of viruses]. | | 1974 | 4615467 |
| comparison of chlamydia psittaci isolates by dna restriction endonuclease analysis. | preparations of dna from 12 chlamydia psittaci isolates and one chlamydia trachomatis strain were compared by restriction endonuclease analysis. polyacrylamide gel electrophoresis, followed by silver staining, resulted in optimal resolution of fragments generated by digestion. by this technique, four distinct electropherotypes were demonstrated when ovine abortion, ovine arthritis, and avian and cal10 strains of c. psittaci were examined. minor profile differences allowed the discrimination of a ... | 1984 | 6086526 |
| chlamydial endocarditis. | | 1980 | 6101465 |
| serological cross-reactions of lipid a components of lipopolysaccharides isolated from chlamydia psittaci and coxiella burnetii. | | 1980 | 6107040 |
| psittacosis of non-avian origin. | | 1984 | 6148539 |
| [allergic skin test with a group-specific chlamydial allergen in nongonococcal urethritis]. | | 1980 | 6154365 |
| [chlamydial polyarthritis in calves]. | investigations were carried out on some farms to establish the presence of chlamydia organisms in spontaneous cases of polyarthritis in calves. use was made of various methods--chick embryos, light and electron microscopy, and the complement-fixation test (cft). as many as 9 c. psittaci strains were isolated from synovial liquid of calves affected with polyarthritis from 6 farms in several districts of the country. the isolated strains were identified morphologically by means of electron and lig ... | 1981 | 6171089 |
| a rapid method for staining inclusions of chlamydia psittaci and chlamydia trachomatis. | a new staining method was developed for the detection of inclusions of chlamydia psittaci and chlamydia trachomatis inclusions in cell cultures. using a combination of methyl green and neutral red stains and washing at ph 5.0, inclusions were stained red while cell cytoplasm was pale pink and cell nuclei were pale green. the method was significantly better than giemsa staining and comparable to immunofluorescence for detecting c psittaci inclusions. its sensitivity for detection c trachomatis in ... | 1982 | 6177720 |
| electron microscopic observations of surface projections and related intracellular structures of chlamydia organisms. | | 1981 | 6177811 |
| chlamydia psittaci: inclusion vacuole ultrastructure. | the inclusion ultrastructure of fibroblasts infected with chlamydia psittaci (6bc) was studied. electron microscopic techniques were used which permitted the observation of whole infected host cells and 1.0-micron sectioned preparations. it was shown that the cytoplasmic inclusion vacuoles of infected cells contained interconnecting structures within which chlamydiae reproduce. | 1980 | 6250694 |
| [main respiratory virus and chlamydia psittaci antibody survey by passive hemagglutination on bovine serum from togo]. | | 1980 | 6256826 |
| host influence on the density of chlamydiae in renografin gradients. | egg-grown and l cell-grown c. psittaci 6bc strains formed two bands when centrifuged through preformed renografin gradients. no additional bands were observed by extending the time of centrifugation. particles present in the bands which were collected from the renografin gradients were examined by electron microscopy. purified elementary bodies from both egg- and l cell-grown sources were located in a lower, minor bind; egg-derived elementary bodies showed a higher density (p < 0.01) than elemen ... | 1980 | 6256995 |
| isolation and electron microscopic observations of intracytoplasmic inclusions containing chlamydia psittaci. | intracytoplasmic inclusions containing chlamydia psittaci were isolated by a newly established method. infected l-cells at 20 h after infection were suspended in 0.25 m sucrose-tris(hydroxymethyl)aminomethane buffer containing ethylene-diaminetetraacetic acid, homogenized in a dounce tissue grinder, and filtered through a 2,000-mesh screen. isolated inclusions were stabilized in 5% bovine serum albumin in 10 mm tris(hydroxymethyl)aminomethane buffer. electron microscopic observations revealed th ... | 1981 | 6257643 |
| persistent infection of mouse fibroblasts (l cells) with chlamydia psittaci: evidence for a cryptic chlamydial form. | when monolayers of mouse fibroblasts (l cells) were infected with enough chlamydia psittaci (strain 6bc) to destroy most of the host cells, 1 in every 10(5) to 10(6) originally infected cells gave rise to a colony of l cells persistently infected with strain 6bc. in these populations, the density of l cells and 6bc fluctuated periodically and reciprocally as periods of host cell increase were followed by periods of parasite multiplication. successive cycles of l-cell and 6bc reproduction were su ... | 1980 | 6262243 |
| adenine nucleotide and lysine transport in chlamydia psittaci. | isolated reticulate bodies of chlamydia psittaci were found to transport atp and adp by an atp-adp exchange mechanism. atp uptake activity was not detected in elementary bodies. the apparent km of transport for both atp and adp was approximately 5 microm, and the calculated vmax for both was about 1 nmol of nucleotide transported per min per mg of protein. adp competitively inhibited atp transport with a ki of 4.5 microm. other nucleotides tested had no effect on the uptake of atp. a magnesium-d ... | 1982 | 6279566 |
| characterization of chlamydia dna by restriction endonuclease cleavage. | the dna from six serovars of chlamydia trachomatis, lymphogranuloma venereum (lgv) i, lgv ii, lgv iii, b, c, and d, and from chlamydia psittaci was extracted, treated with restriction endonuclease enzymes, and run on agarose gels. by using this technique, the dna of c. trachomatis could be clearly differentiated from c. psittaci dna. a comparison of the dna from the different serovars of c. trachomatis revealed similar patterns with and without detectable differences. lgv i, lgv ii, lgv iii, b, ... | 1983 | 6307876 |
| chlamydiae and viruses causing human sexually transmitted diseases - analogies with infections in animals: a review. | | 1983 | 6308254 |
| gamma-interferon is the factor in lymphokine that activates human macrophages to inhibit intracellular chlamydia psittaci replication. | we have demonstrated previously that mitogen-induced lymphokines activate human monocyte-derived macrophages to inhibit the intracellular replication of chlamydia psittaci. to identify the factor(s) in crude lymphokines responsible for this antimicrobial effect, we tested human con a-induced lymphokines for interferon activity. we also attempted to neutralize the lymphokines with a monoclonal antibody directed against human gamma-interferon and examined the ability of partially purified human ga ... | 1983 | 6313807 |
| chlamydial infections in obstetrics and gynecology. | although chlamydia trachomatis causes important diseases in both men and women, this review focuses on the genital tract disease associated with chlamydial infection in women and on neonatal chlamydial infection. 8 of the 15 serotypes of c. trachomatis are sexually transmitted agents. the unique growth cycle which distinguishes the chlamydiae from all other organisms is described and they are compared to bacteria and viruses. the prevalence and risk factors for chlamydial infections are then ... | 1983 | 6340891 |
| the genus-specific antigen of chlamydia: resemblance to the lipopolysaccharide of enteric bacteria. | a strong immunological cross-reaction between a major glycolipid antigen of chlamydia and the innermost (re) core of the lipopolysaccharide of enteric bacteria was demonstrated with the aid of mutants in which the re structure is exposed. the chlamydial glycolipid resembled the re lipopolysaccharide in molecular size, solubility, and endotoxic properties and may thus be functionally equivalent to lipopolysaccharide, an essential and characteristic component of the outer membrane of gram-negative ... | 1983 | 6344216 |
| chlamydial classification, development and structure. | | 1983 | 6347317 |
| chlamydial serology. | | 1983 | 6347331 |
| isolation of chlamydia psittaci from naturally infected african clawed frogs (xenopus laevis). | an inclusion-forming agent was isolated from the livers of commercially raised african clawed frogs (xenopus laevis) involved in an epizootic of high morbidity and mortality. original isolation was made in mccoy cells. this agent was identified as chlamydia psittaci based on the formation of typical intracytoplasmic inclusions which developed within 48 h, were not stained by iodine, and were resistant to sulfadiazine. the isolate from one particular frog (designated as strain 178) was further st ... | 1983 | 6347897 |
| [enzyme immunoassay for detection of igg and igm antibodies against chlamydia psittaci in the pigeon]. | | 1983 | 6351502 |
| [relationship between antibody status and the course of ornithosis and salmonellosis infection in racing pigeons]. | | 1983 | 6351835 |
| high prevalence of antibodies to chlamydia trachomatis in a northern canadian community. | | 1983 | 6354412 |
| effects of cortisone, cytochalasin b and cycloheximide on strains of chlamydia psittaci in cell cultures. | vero and mccoy cell cultures were tested for their susceptibility to chlamydia psittaci in the presence of several antimetabolites, such as cortisone acetate, cytochalasin b and cycloheximide. vero cells were more susceptible than the mccoy cell cultures as demonstrated by cytopathic changes, fluorescent antibody activity, and titer of c. psittaci. although all of the antimetabolites increased these parameters, a mixture of cortisone and cytochalasin b was the most effective. | 1983 | 6357413 |
| enzyme-linked immunosorbent assay for the detection of antibody to chlamydia trachomatis and chlamydia psittaci. | an enzyme-linked immunosorbent assay (elisa) was developed with use of a strain of chlamydia trachomatis, lymphogranuloma venereum serotype 2. the elisa reactions were monitored by absorbance at 492 nm in a spectrophotometer. a positive control method based on one serum dilution was used to assign elisa titers to the sera. sera that had been tested with use of complement fixation (cf) and microimmunofluorescence (mif) tests were examined with elisa. elisa was more sensitive than the cf test and ... | 1983 | 6358382 |
| [chlamydiosis in animals]. | in this brief review, the authors summarize the state of knowledge on chlamydial infections in birds and mammals, in particular those occurring in domestic animals. chlamydia psittaci is responsible for a variety of syndromes in veterinary medicine, some of which may be of great interest in comparative pathology (polyarthritis, conjunctivitis, . . .). the frequency of latent infections, the difficulties involved in the diagnosis and control of chlamydial infections and the transmissibility of c. ... | 1983 | 6364314 |
| simple transport medium for the isolation of chlamydia psittaci from clinical material. | infectious elementary bodies of chlamydia psittaci in tissue samples from field cases of enzootic abortion were placed in five different transport media (a to e). in one medium, in the absence of refrigerative storage, the organism remained viable for 30 days and at 4 degrees c for 34 days. this was medium d; it consisted of sucrose (74.6 g/litre), k2hpo4 (1.237 g/litre), l-glutamic acid (0.721 g/litre), fetal calf serum (10 per cent v/v), vancomycin and streptomycin (100 micrograms/ml) and nyst ... | 1983 | 6364542 |
| goat serums for fluorescent antibody conjugates to chlamydial antigens. | serums from goats hyperimmunized with chlamydia psittaci consistently produce antichlamydial fluorescent antibody conjugate of high titer. the titer of the fluorescent antibody conjugate prepared from a given serum correlated well with the titer obtained by agar gel precipitin, but not with the complement fixation. the agar gel precipitin test can be used to predict whether a given serum is satisfactory for use in production of a conjugate for direct fluorescent antibody tests. serums with an ag ... | 1984 | 6372973 |