| cinnamaldehyde and cinnamaldehyde derivatives reduce virulence in vibrio spp. by decreasing the dna-binding activity of the quorum sensing response regulator luxr. | to date, only few compounds targeting the ai-2 based quorum sensing (qs) system are known. in the present study, we screened cinnamaldehyde and substituted cinnamaldehydes for their ability to interfere with ai-2 based qs. the mechanism of qs inhibition was elucidated by measuring the effect on bioluminescence in several vibrio harveyi mutants. we also studied in vitro the ability of these compounds to interfere with biofilm formation, stress response and virulence of vibrio spp. the compounds w ... | 2008 | 18793453 |
| in vitro evaluation on adherence and antimicrobial properties of a candidate probiotic clostridium butyricum cb2 for farmed fish. | to characterize the antimicrobial and adhesion ability of candidate probiotic clostridium butyricum cb2 for farmed fish in vitro. | 2008 | 18795975 |
| cd8alpha and cd8beta in atlantic halibut, hippoglossus hippoglossus: cloning, characterization and gene expression during viral and bacterial infection. | cd8 is expressed on cytotoxic t-cells where it functions as a co-receptor for the tcr by binding to mhc class i proteins that present peptides on the cell surface. in this study we describe the cloning and sequencing of full length cdnas encoding cd8alpha and cd8beta from atlantic halibut (hippoglossus hippoglossus l.) and subsequent isolation and characterization of the cd8alpha and cd8beta genes. the predicted halibut cd8alpha and cd8beta proteins are similar to those of mammals and other fish ... | 2008 | 18801441 |
| isolation and structural characterisation of two antibacterial free fatty acids from the marine diatom, phaeodactylum tricornutum. | one solution to the global crisis of antibiotic resistance is the discovery of novel antimicrobial compounds for clinical application. marine organisms are an attractive and, as yet, relatively untapped resource of new natural products. cell extracts from the marine diatom, phaeodactylum tricornutum, have antibacterial activity and the fatty acid, eicosapentaenoic acid (epa), has been identified as one compound responsible for this activity. during the isolation of epa, it became apparent that t ... | 2008 | 18813920 |
| cdna cloning, characterization and mrna expression of a pacifastin light chain gene from the chinese mitten crab eriocheir sinensis. | the pacifastin family, characterized by several conserved arrays of six cysteine residues, is a newly identified serine protease inhibitor (spi) family discovered uniquely in arthropods and plays important roles in multiple biological processes. in the present study, the full-length cdna of a pacifastin light chain (designated esplc) was cloned from the chinese mitten crab eriocheir sinensis by expressed sequence tags (ests) and pcr techniques. the 1036bp esplc cdna contained an 831bp open readi ... | 2008 | 18817876 |
| a cyclophilin a inducible expressed in gonad of zhikong scallop chlamys farreri. | cyclophilin a (cypa), a receptor for the immunosuppressive agent cyclosporin a (csa), is a cis-trans peptidyl-prolyl isomerase (ppiase) which accelerates the cis-trans isomerization of prolyl-peptide bonds, interacts with a variety of proteins and therefore regulates their activities. one cypa (designated cfcypa) cdna was cloned from chlamys farreri by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of cfcypa consisted of 1,248 nucleotide ... | 2009 | 18819019 |
| molecular cloning and characterization of the translationally controlled tumor protein from fenneropenaeus chinensis. | the translationally controlled tumor protein (tctp) is a multi-functioning protein that performs vital roles, particularly in various complicated life processes. in this study, a new tctp cdna was cloned from fenneropenaeus chinensis and hence was designated as fc-tctp. its length is 711 bp, and it is characterized by 507-bp open reading frame that encodes a deduced 168-amino acid protein, including a tctp domain. moreover, this study analyzed the expression patterns of this gene when it respond ... | 2009 | 18853281 |
| differential involvement of mussel hemocyte sub-populations in the clearance of bacteria. | mussels are filter-feeders living in a bacteria-rich environment. we have previously found that numerous bacterial species are naturally present within the cell-free hemolymph, including several of the vibrio genus, whereas the intra-cellular content of hemocytes was sterile. when bacteria were injected into the circulation of the mussel, the number of living intra-hemocyte bacteria dramatically increased in less than an hour, suggesting intense phagocytosis, then gradually decreased, with no vi ... | 2008 | 18854215 |
| phaeobacter and ruegeria species of the roseobacter clade colonize separate niches in a danish turbot (scophthalmus maximus)-rearing farm and antagonize vibrio anguillarum under different growth conditions. | members of the roseobacter clade colonize a spanish turbot larval unit, and one isolate (phaeobacter strain 27-4) is capable of disease suppression in in vivo challenge trials. here, we demonstrate that roseobacters with antagonistic activity against vibrio anguillarum also colonize a danish turbot larval farm that relies on a very different water source (the danish fiord limfjorden as opposed to the galician atlantic ocean). phylogenetic analyses based on 16s rrna and gyrase b gene sequences re ... | 2008 | 18952864 |
| molecular characterization of the tonb2 protein from the fish pathogen vibrio anguillarum. | in the fish pathogen vibrio anguillarum the tonb2 protein is essential for the uptake of the indigenous siderophore anguibactin. here we describe deletion mutants and alanine replacements affecting the final six amino acids of tonb2. deletions of more than two amino acids of the tonb2 c-terminus abolished ferric-anguibactin transport, whereas replacement of the last three residues resulted in a protein with wild-type transport properties. we have solved the high-resolution solution structure of ... | 2009 | 18973471 |
| specific and natural antibody response of cod juveniles vaccinated against vibrio anguillarum. | the purpose of the present study was to study specific and natural antibody levels in individual cod juveniles before and after being vaccinated against vibrio anguillarum. different vaccine preparations and vaccination regimes, i.e. bathing, dipping, i.p. injection or combination of treatments were employed and the performance of different groups to bath challenge by the bacterium tested. antibody responses to v. anguillarum antigens in groups vaccinated by bathing and/or dipping were negligibl ... | 2009 | 18977447 |
| responses of alternative complement expression to challenge with different combinations of vibrio anguillarum, escherichia coli and staphylococcus aureus: evidence for specific immune priming in amphioxus branchiostoma belcheri. | the existence of specific immunological priming in protection upon secondary exposure in invertebrates remains controversial. by exploring the changes in the expression patterns of bf, c3 and c6, key genes involved in the alternative complement pathway (ap), after challenge with different combinations of three bacteria vibrio anguillarum, escherichia coli and staphylococcus aureus, we show that re-exposure to the same species of bacteria or the different species of the same (gram-negative or gra ... | 2009 | 18983925 |
| molecular cloning and characterization of peroxiredoxin 6 from chinese mitten crab eriocheir sinensis. | peroxiredoxin is a superfamily of antioxidative proteins that play important roles in protecting organisms against the toxicity of reactive oxygen species (ros). in this study, the full-length cdna encoding peroxiredoxin 6 (designated esprx6) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches. the full-length cdna of esprx6 was of 1076 bp, containing a 5' untranslated region (utr) of 69 bp, a 3' utr of 347 bp with a poly (a) tail, a ... | 2009 | 18992822 |
| screening and characterisation of potentially pathogenic bacteria associated with atlantic cod gadus morhua larvae: bath challenge trials using a multidish system. | in intensive aquaculture systems, high concentrations of nutrients and high densities of fish larvae provide favorable conditions for opportunistic pathogenic bacteria to flourish. we screened potentially pathogenic bacterial strains isolated from moribund atlantic cod gadus morhua larvae, pollack pollachius pollachius, coalfish pollachius virens, atlantic halibut hippoglossus hippoglossus, rotifers, algae and water samples from different hatcheries. three identical challenge experiments tested ... | 2008 | 18998585 |
| a fibrinogen-related protein from bay scallop argopecten irradians involved in innate immunity as pattern recognition receptor. | the family of fibrinogen-related proteins (freps) is a group of proteins with fibrinogen-like domains. many members of this family play important roles as pattern recognition receptors in innate immune responses. the cdna of bay scallop argopecten irradians frep (designated as aifrep) was cloned by rapid amplification of cdna ends (race) method based on the expressed sequence tag (est). the full-length cdna of aifrep was of 990bp. the open reading frame encoded a polypeptide of 251 amino acids, ... | 2009 | 19013249 |
| the expression of peptidoglycan recognition protein-s1 gene in the scallop chlamys farreri was enhanced after a second challenge by listonella anguillarum. | a more rapid and powerful response against repeated exposure of same pathogen in vertebrates is usually considered as the reflection of immunological memory, but it is not well understood in invertebrates. in the present study, the temporal expression profiles of chlamys farreri peptidoglycan recognition protein-s1 (cfpgrp-s1) gene after two challenges of listonella anguillarum were examined to evaluate priming response in scallops. the up-regulation of cfpgrp-s1 mrna occurred 3h earlier, and th ... | 2009 | 19022261 |
| molecular characterisation of a goose-type lysozyme gene in atlantic cod (gadus morhua l.). | lysozymes are antibacterial enzymes important in the innate immune defense of several animal phyla. an atlantic cod goose-type (g-type) lysozyme est was identified in a suppression subtractive hybridisation (ssh) cdna library and the full-length cdna (codg1) was obtained by race-pcr. the lysozyme gene is organised in five exons and four introns similar to g-type lysozyme genes in other fish species. two different cod lysozyme transcripts, named codg1 and codg2, seem to be produced by the use of ... | 2009 | 19041261 |
| a cd4 homologue in atlantic halibut (hippoglossus hippoglossus): molecular cloning and characterisation. | cd4 is expressed mainly on the surface of t helper cells, where it functions as a co-receptor for the t-cell receptor (tcr) by binding to mhc class ii proteins. in this study we describe the cloning and sequencing of a cdna encoding a cd4 homologue from atlantic halibut (hippoglossus hippoglossus l.) and the subsequent characterisation of the cd4 genomic sequence. the predicted cd4 protein has four extracellular immunoglobulin-like (ig-like) domains (d1-d4), and was structurally similar to other ... | 2009 | 19070667 |
| cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop argopecten irradians. | extracellular superoxide dismutase (ecsod) is a major extracellular antioxidant enzyme that protects organs from damage by reactive oxygen species (ros). we cloned a novel ecsod from the bay scallop argopecten irradians (aiecsod) by 3' and 5' race. the full-length cdna of aiecsod was 893bp with a 657bp open reading frame encoding 218 amino acids. the deduced amino acid sequence contained a putative signal peptide of 20 amino acids, and sequence comparison showed that aiecsod had low degree of ho ... | 2009 | 19084069 |
| comparison of antibody responses in atlantic cod (gadus morhua l.) to vibrio anguillarum, aeromonas salmonicida and francisella sp. | bacterial diseases such as vibriosis, atypical furunculosis and francisellosis, are registered as an increasing problem in cod farming in norway. in order to develop efficient vaccines against diseases it is of interest to investigate if the cod immune system differentiates between various serotypes of vibrio anguillarum and variants of aeromonas salmonicida associated with the diseases by raising specific antibody responses. cod of the same origin were shown to raise significant responses to v. ... | 2009 | 19084603 |
| vibriosis as an epizootic disease of rainbow trout (onchorynchus mykiss) in turkey. | vibrio anguillarum o1 was isolated from diseased cultured rainbow trout oncorhynchus mykiss, in the southern aegean of turkey with lesions and signs of vibriosis. gram (-), motile and curved rods from diseased rainbow trout were identified after physiological, biochemical and slide agglutination tests as v. anguillarum o1. koch postulates were fulfilled with the isolated bacteria. the antibiotic susceptible test results showed that v. anguillarum strains were susceptibility to amoxycillin, ampyc ... | 2007 | 19086527 |
| molecular characterisation and expression analysis of interferon gamma in atlantic cod (gadus morhua). | interferon gamma (ifn-gamma) has important roles in both innate and adaptive immune responses. in this study, the cdna and genomic sequences of atlantic cod ifn-gamma were cloned and found to encode a putative protein containing 194 amino acids with a 24 amino acid signal peptide sequence. the gene is composed of four exons and three introns similar to ifn-gamma genes of other vertebrates. the cod ifn-gamma showed only 14-29% amino acid identity with other fish ifn-gamma and 9-17% identity with ... | 2009 | 19100838 |
| molecular and genetic characterization of the tonb2-cluster ttpc protein in pathogenic vibrios. | ttpc is a fourth required protein in the tonb2 energy transduction system in vibrio anguillarum. ttpc is necessary for iron transport mediated by the tonb2 system and is highly conserved in all pathogenic vibrio species studied to date as well as several marine organisms. we show here that the ttpc proteins from selected pathogenic vibrio species can function with the tonb2 system of v. anguillarum to allow iron transport mediated by a chimeric tonb2 system where the native exbb2, exbd2 and tonb ... | 2009 | 19130262 |
| rpon gene, rapd profile, antimicrobial resistance and plasmids of vibrio anguillarum isolates from vibriosis infected penaeus monodon. | to evaluate the diversity of vibrio anguillarum isolates from vibriosis-infected penaeus monodon collected from east coast of india. | 2008 | 19146525 |
| the polymorphism of lysozyme gene in zhikong scallop (chlamys farreri) and its association with susceptibility/resistance to listonella anguillarum. | lysozyme functions as a crucial biodefence effector against the infection of bacterial pathogens in innate immunity. the nucleotide sequence polymorphisms in promoter region of a nuclear goose type lysozyme gene from zhikong scallop chlamys farreri (designated as cflysg) were investigated to explore their association with susceptibility/resistance to listonella anguillarum infection. eight sites of single nucleotide polymorphisms (snps) and two sites of insert-deletion (ins-del) polymorphisms we ... | 2009 | 19154789 |
| alternative complement activity in the egg cytosol of amphioxus branchiostoma belcheri: evidence for the defense role of maternal complement components. | the eggs in most invertebrates are fertilized externally, and therefore their resulting embryos are exposed to an environment full of microbes, many of which are pathogens capable of killing other organisms. how the developing embryos of invertebrates defend themselves against pathogenic attacks is an intriguing question to biologists, and remains largely unknown. | 2009 | 19156196 |
| siderophore production by the marine-derived aureobasidium pullulans and its antimicrobial activity. | over 300 yeast strains isolated from different marine environments were screened for their ability to produce siderophore. among them, only the yeast strain hn6.2 which was identified to be aureobasidium pullulans was found to produce high level of the siderophore. under the optimal conditions, this yeast strain could produce 1.1mg/ml of the siderophore. the crude siderophore produced by the yeast strain hn6.2 was able to inhibit cell growth of vibrio anguillarum and vibrio parahaemolyticus, iso ... | 2009 | 19162476 |
| cflec-4, a multidomain c-type lectin involved in immune defense of zhikong scallop chlamys farreri. | c-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. in this study, a novel multidomain c-type lectin gene from scallop chlamys farreri (designated as cflec-4) was cloned by race approach based on est analysis. the full-length cdna of cflec-4 was of 2086 bp. the open reading frame was of 1830bp and encoded a polypeptide of 609 amino acids, including a signal sequence and four dissimilar carbohydrate-recognition domains (crds). the ... | 2009 | 19185588 |
| real-time pcr detection and quantification of fish probiotic phaeobacter strain 27-4 and fish pathogenic vibrio in microalgae, rotifer, artemia and first feeding turbot (psetta maxima) larvae. | to develop a sybr green quantitative real-time pcr protocol enabling detection and quantification of a fish probiotic and two turbot pathogenic vibrio spp. in microcosms. | 2009 | 19187159 |
| generation of vibrio anguillarum ghost by coexpression of phix 174 lysis e gene and staphylococcal nuclease a gene. | vibrio anguillarum ghosts (vag) were generated, for the first time, using a conjugation vector containing a ghost bacteria inducing cassette, prk-lambdap(r)-ci-elysis, in which the expression of phix174 lysis gene e was controlled by the p ( r )/ci regulatory system of lambda phage. by scanning electron microscopy, holes ranging 80-200 nm in diameter were observed in the vag. to avoid the presence of bacterial genomic dna and an antibiotic resistance gene in the final vag product, we constructed ... | 2009 | 19191038 |
| development of a bacterial challenge test for gnotobiotic sea bass (dicentrarchus labrax) larvae. | the use of probiotic microorganisms in aquaculture is gaining a lot of interest. gnotobiotic model systems are required in order to fully understand the effects and modes-of-action of these microorganisms, as the native microbial communities present in non-sterile animals can lead to false conclusions. in this study, a gnotobiotic sea bass larvae (dicentrarchus labrax) test system was developed. in order to obtain bacteria-free animals, the eggs were disinfected with glutaraldehyde and subsequen ... | 2009 | 19196282 |
| identification of vibrio anguillarum outer membrane vesicles related to immunostimulation in the japanese flounder, paralichthys olivaceus. | we identified outer membrane vesicle (omv) production in vibrio anguillarum o1, a major fish pathogen that causes vibriosis, and characterized the omvs. they were produced during normal growth, and were appeared as spherical vesicle fractions. the protein profile of the omvs was similar to that of the outer membrane proteins, and the 38-kda major protein band of omv was identified as ompu. the omvs had enzyme activity of metalloprotease, hemolysin, and phospholipase, and stimulated the productio ... | 2009 | 19202277 |
| detection of vibrio anguillarum by pcr amplification of the empa gene. | | 2009 | 19236556 |
| immunotoxicological effects of a sub-chronic exposure to selected current-use pesticides in rainbow trout (oncorhynchus mykiss). | many current-use pesticides (cups) are found at increasing concentrations in aquatic environments, yet relatively little is known about their effects on the immune system of fish. we examined the in vivo effects of three pesticides (chlorothalonil, cypermethrin and pentachlorophenol) on the immune system of juvenile rainbow trout (oncorhynchus mykiss) by assessing a suite of innate immune function tests, as well as a host resistance test using listonella anguillarum. increased activity of phagoc ... | 2009 | 19237205 |
| salt-responsive outer membrane proteins of vibrio anguillarum serotype o1 as revealed by comparative proteome analysis. | vibrio anguillarum is a universal marine pathogen causing vibriosis. vibrio anguillarum encounters different osmolarity conditions between seawater and hosts, and its outer membrane proteins (omps) play a crucial role in the adaptation to changes of the surroundings. in the present study, proteomic approaches were applied to investigate the salt-responsive omps of v. anguillarum. | 2009 | 19245402 |
| a novel c-type lectin (cflec-3) from chlamys farreri with three carbohydrate-recognition domains. | c-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. in this study, the gene of a c-type lectin with multiple carbohydrate-recognition domains (crds) from scallop chlamys farreri (designated as cflec-3) was cloned by rapid amplification of cdna ends (race) approach based on expression sequence tag (est) analysis. the full-length cdna of cflec-3 was of 2256 bp. the open reading frame encoded a polypeptide of 516 amino acids, includ ... | 2009 | 19264137 |
| a thioredoxin with antioxidant activity identified from eriocheir sinensis. | thioredoxin, with a redox-active disulfide/dithiol in the active site, is the major ubiquitous disulfide reductase responsible for maintaining proteins in their reduced state. in the present study, the cdna encoding thioredoxin-1 (designated estrx1) was cloned from chinese mitten crab eriocheir sinensis by using rapid amplification of cdna ends (race) approaches. the full-length cdna of estrx1 was of 641 bp, containing a 5' untranslated region (utr) of 17 bp, a 3' utr of 306 bp with a poly (a) t ... | 2009 | 19269333 |
| fvta is the receptor for the siderophore vanchrobactin in vibrio anguillarum: utility as a route of entry for vanchrobactin analogues. | some strains of vibrio anguillarum, the causative agent of vibriosis in a variety of marine animals, produce a catechol-type siderophore named vanchrobactin. the biosynthetic pathway and regulation of vanchrobactin are quite well understood. however, aspects concerning its entry into the cell have remained uncharacterized. in the present study we characterized two genes, fvta and orf13, encoding potential tonb-dependent ferric-vanchrobactin receptors in serotype o2 v. anguillarum strain rv22. we ... | 2009 | 19270115 |
| decreased mortality of lake michigan chinook salmon after bacterial kidney disease challenge: evidence for pathogen-driven selection? | in the late 1960s, chinook salmon oncorhynchus tshawytscha from the green river, washington, were successfully introduced into lake michigan. during spring from 1988 to 1992, large fish die-offs affecting chinook salmon occurred in the lake. multiple ecological factors probably contributed to the severity of the fish kills, but the only disease agent found regularly was renibacterium salmoninarum, the causative agent of bacterial kidney disease. in this study, survival after challenge by r. salm ... | 2008 | 19306612 |
| profiling gene expression in the spleen of atlantic cod, gadus morhua upon vaccination with vibrio anguillarum antigen. | the mechanisms of physiological responses in atlantic cod, gadus morhua following vaccination with a heat-killed vibrio anguillarum were investigated by transcriptome analysis of spleen tissues. semi-quantitative rt-pcr of several genes involved in immune defense, inflammation, antioxidant defense and glucose transport were determined in vaccinated fish at 1, 3, 7 and 10 days after vaccination (dpv)and compared with sham-injected fish. transcript levels of the selected genes involved in bacteria ... | 2009 | 19318130 |
| expressed sequence tags from the zhikong scallop (chlamys farreri): discovery and annotation of host-defense genes. | a high-quality cdna library was constructed from whole body tissues of the zhikong scallop, chlamys farreri, challenged by listonella anguillarum. a total of 5720 clones were sequenced, yielding 5123 expressed sequence tags (ests). among the 3326 unique genes identified, 2289 (69%) genes had no significant (e-value < 1e-5) matches to known sequences in public databases and 194 (6%) matched proteins of unknown functions. the remaining 843 (25%) genes that exhibited homology with genes of known fu ... | 2009 | 19328855 |
| myxinidin, a novel antimicrobial peptide from the epidermal mucus of hagfish, myxine glutinosa l. | fish epidermal mucus contains innate immune components that provide a first line of defense against various infectious pathogens. this study reports the bioassay-guided fractionation and characterization of a novel antimicrobial peptide, myxinidin, from the acidic epidermal mucus extract of hagfish (myxine glutinosa l.). edman sequencing and mass spectrometry revealed that myxinidin consists of 12 amino acids and has a molecular mass of 1,327.68 da. myxinidin showed activity against a broad rang ... | 2009 | 19330556 |
| clip domain serine protease and its homolog respond to vibrio challenge in chinese white shrimp, fenneropenaeus chinensis. | clip domain serine proteases and their homologs are involved in invertebrate innate immunity, including hemolymph coagulation, antimicrobial peptide synthesis, cell adhesion, and melanization. recognition of pathogens by pattern recognition receptors can trigger activation of a serine protease cascade. we report here the cdna cloning of a serine protease (fcsp) and a serine protease homolog (fcsph) from chinese white shrimp, fenneropenaeus chinensis. both fcsp and fcsph possess a clip domain at ... | 2009 | 19332134 |
| bacteriocin production by indigenous marine catfish associated vibrio spp. | fifty strains of genus vibrio were isolated (identified) from healthy and diseased marine catfish(es). the isolates were screened for bacteriocin (vibriocin) production. about 32% isolates were found bacteriocin producers. the best producer was identified as vibrio anguillarum avp10. the maximum production of vibriocin avp10 was manifested at 29 degrees c at ph 7, after 18-20 h of incubation. vibriocin activity was enhanced in the presence of citrate-phosphate buffer. the vibriocin avp10 withsta ... | 2009 | 19339226 |
| isolation and characterization of toll-like receptor 9 in half-smooth tongue sole cynoglossus semilaevis. | toll-like receptors (tlrs) are considered as key sensors to trigger the host's innate immune system and adaptive immune responses by recognizing various pamps and initiating signal transduction. tlr9, as a member of tlr family, mediates the recognition of unmethylated cpg dinucleotide motifs commonly found in both bacterial and viral genomes. in the current study, the tlr9 gene was isolated from one of flatfish species, half-smooth tongue sole (cynoglossus semilaevis). in the 4588 bp genomic seq ... | 2009 | 19340952 |
| functional and molecular immune response of mediterranean mussel (mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria. | the effect of live bacteria (micrococcus lysodeikticus and vibrio anguillarum), and pamps (poly i:c, zymosan, lps, lta and cpg) on the production of intermediate toxic radicals (respiratory burst activity and production of nitric oxide) and mytilin b, myticin c and lysozyme gene expression was studied in vivo and in vitro. in vitro, bacteria were able to modulate the haemocytes' respiratory burst activity, being significantly increased after 6 h of incubation. the effect of pathogen-associated m ... | 2009 | 19340955 |
| vibrios as causal agents of zoonoses. | vibrios are gram-negative rod-shaped bacteria that are widespread in the coastal and estuarine environments. some species, e.g. vibrio anguillarum and v. tapetis, comprise serious pathogens of aquatic vertebrates or invertebrates. other groups, including grimontia (=vibrio) hollisae, photobacterium (=vibrio) damselae subsp. damselae, v. alginolyticus, v. harveyi (=v. carchariae), v. cholerae, v. fluvialis, v. furnissii, v. metschnikovii, v. mimicus, v. parahaemolyticus and v. vulnificus, may cau ... | 2010 | 19342185 |
| protection of japanese flounder (paralichthys olivaceus) against vibrio anguillarum with a dna vaccine containing the mutated zinc-metalloprotease gene. | vibrio anguillarum is one of the causative agents of vibriosis, a systemic disease of fish characterized by acute hemorrhagic septicemia. the extracellular zinc metalloprotease (empa) is a putative virulence factor involved in pathogenicity of v. anguillarum. here we described the results of immunization against v. anguillarum with the plasmid expressing the mutated empa (m-empa7), which had no protelytic activity or cytotoxicity. in vitro protein expression of m-empa7 gene was determined by flu ... | 2009 | 19356619 |
| a selenium-dependent glutathione peroxidase (se-gpx) and two glutathione s-transferases (gsts) from chinese shrimp (fenneropenaeus chinensis). | glutathione s-transferases (gsts) and glutathione peroxidases (gpxs) are essential components of cellular detoxification systems that defend cells against reactive oxygen species (ross). two gstgenes have recently been cloned from fenneropenaeus chinensis and blast p analysis shows that one gst, designated fcmugst, is similar to members of mugst while the other has similarities to thetagst (fcthetagst). a selenium-dependent glutathione peroxidase (se-gpx) has also been cloned from f. chinensis. ... | 2009 | 19358336 |
| trbp homolog interacts with eukaryotic initiation factor 6 (eif6) in fenneropenaeus chinensis. | the hiv transactivating response rna-binding protein (trbp) plays an important role in many biological processes. we have cloned three cdnas from newly identified genes in the trbp family from fenneropenaeus chinensis. these genes have been designated fc-trbp1-3. recombinant fc-trbp1, which was produced in escherichia coli, was used for panning of a t7 phage display library of the chinese shrimp hemocytes. from this panning, fc-eukaryotic initiation factor 6 (fc-eif6) was isolated and sequenced. ... | 2009 | 19380771 |
| identification of a cathepsin d potentially involved in h2a cleavage from scallop chlamys farreri. | we report here a cdna and its deduced amino acid sequence encoding a cathepsin d-like, aspartic protease from chlamys farreri (denoted as cfcd) by expressed sequence tag and rapid amplification of cdna ends techniques. the cdna of cfcd consisted of 1,810 nucleotides with a canonical polyadenylation signal sequence aataaa and a polya tail, encoding a short signal peptide of 18 amino acids, a pro-enzyme peptide of 29 amino acid residues, and a mature enzyme of 349 residues. the deduced amino acid ... | 2010 | 19381870 |
| functional differential immune responses of mytilus galloprovincialis to bacterial challenge. | bivalves are filter-feeders that can accumulate large numbers of bacteria, in particular vibrio species; these can persist within bivalve tissues largely depending on their sensitivity to the hemolymph bactericidal activity. in this work, functional parameters of the hemolymph of mytilus galloprovincialis were evaluated in response to in vivo challenge with different bacteria (gram(-) vibrio anguillarum and v. splendidus, gram+ micrococcus lysodeikticus). mussels were injected with heat-killed b ... | 2009 | 19393331 |
| evaluation of candidate reference genes for qpcr during ontogenesis and of immune-relevant tissues of european seabass (dicentrarchus labrax). | the expression level of mrna can vary significantly in different experimental conditions, such as stress, infection, developmental stage or tissue. suitable reference genes are expected to exhibit constant expression levels. however no single gene is constitutively expressed in all cell types and under all experimental conditions. it has become clear that expression stability of the intended reference gene has to be examined before each experiment. for expression studies using quantitative real- ... | 2009 | 19398033 |
| identification of interleukin-22 in gadoids and examination of its expression level in vaccinated fish. | this paper reports the cloning and sequencing of interleukin (il)-22 in two gadoid fish, cod (gadus morhua) and haddock (melanogrammus aeglefinus). the complete transcript of this gene was 1002 and 1154 bp respectively, of which 492 bp was the open reading frame (orf) in both genes. high amino acid identity (88.3%) was found between these genes but was less than 50% aa identity to other known genes. the gene organisation of haddock il-22 consisted of five exons and four introns, as with other il ... | 2009 | 19403174 |
| intracellular copper/zinc superoxide dismutase from bay scallop argopecten irradians: its gene structure, mrna expression and recombinant protein. | superoxide dismutases are an ubiquitous family of enzymes that function to efficiently catalyze the dismutation of superoxide anions. two unique and highly compartmentalized bay scallop argopecten irradians superoxide dismutases: mnsod and eccuznsod, have been molecularly characterized in our previous study. to complete characterize the sod family in a. irradians, a novel intracellular copper/zinc sod from the a. irradians (ai-iccuznsod) was obtained and characterized. the full-length cdna of ai ... | 2009 | 19426808 |
| two thymosin-repeated molecules with structural and functional diversity coexist in chinese mitten crab eriocheir sinensis. | recently, beta-thymosin-like proteins with multiple thymosin domains (defined as thymosin-repeated proteins) have been identified from invertebrate. in the present study, the cdnas of two thymosin-repeated proteins (designated estrp1 and estrp2) were cloned from chinese mitten crab by expressed sequence tags (est) techniques. blast analysis presented three and two thymosin domains in estrp1 and estrp2, respectively, with the identities amongst the five domains varying from 47% to 100%. both estr ... | 2009 | 19428488 |
| molecular cloning of liver wap65 cdna in ayu (plecoglossus altivelis) and mrna expression changes following listonella anguillarum infection. | the teleost warm temperature acclimation related 65 kda protein (wap65) is a plasma glycoprotein with the potential roles in heat adaptation, heme recycling, immune response and copper metabolism. it is most homologous to the mammalian hemopexin, which is the plasma transporter of heme. a full-length cdna clone of the wap65 gene, 1,534 bp in size, was isolated from the fish ayu (plecoglossus altivelis). its deduced amino acid sequence of 439 residues had 60.4-65.4% and 38.3-47.3% identical to fi ... | 2010 | 19434513 |
| molecular cloning, genomic structure, polymorphism and expression analysis of major histocompatibility complex class iia and iib genes of half-smooth tongue sole (cynoglossus semilaevis). | major histocompatibility complex (mhc) genes play an important role in the immune response of vertebrates. its function is to present foreign peptide to the t-cell. in order to study the function and molecular polymorphism of class ii genes in teleost, the full lengths of mhc class iia and iib cdna were cloned from half-smooth tongue sole by homology cloning and rapid amplification of cdna ends polymerase chain reaction (race-pcr). genomic organizations, molecular polymorphism, and expression pr ... | 2009 | 19442741 |
| a novel c-type lectin (fclec4) facilitates the clearance of vibrio anguillarum in vivo in chinese white shrimp. | c-type lectins play important roles in innate immunity of invertebrates. in the present study, we report a novel c-type lectin, named fclec4, from the chinese white shrimp fenneropenaeus chinensis. fclec4 contains a single carbohydrate recognition domain (crd) with a putative signal peptide. phylogenetic analysis indicated that fclec4 was distant from most reported c-type lectins from shrimps. the expression of fclec4 increased at both mrna and protein level after stimulation of vibrio anguillar ... | 2009 | 19447130 |
| chemical and biological characterization of siderophore produced by the marine-derived aureobasidium pullulans hn6.2 and its antibacterial activity. | after analysis using hplc and electronic ion spray mass spectroscopy, the purified siderophore produced by the marine-derived aureobasidium pullulans hn6.2 was found to be fusigen. the purified desferric fusigen still had strong inhibition of growth of the pathogenic vibrio anguillarum while the fusigen chelated by fe3+ lost the ability to inhibit the growth of the pathogenic bacterium. the added iron in the medium repressed expression of the hydroxylase gene encoding ornithine n5-oxygenase that ... | 2009 | 19459055 |
| the genomic structure, alternative splicing and immune response of chlamys farreri thioester-containing protein. | cftep is a member of thioester-containing protein (tep) family found in zhikong scallop chlamys farreri and is involved in innate immunity against invading microbes. in the present study, the genomic dna of cftep was cloned and characterized. the genomic dna sequence of cftep consisted of 40 exons and 39 introns spanning 35kb with all exon-intron junction sequences agreeing with the gt/ag consensus. the genomic organization of cftep was similar to human and mouse c3 rather than ciona c3-1 and dr ... | 2009 | 19467260 |
| structural characterization of the lipopolysaccharide o-antigen from atypical isolate of vibrio anguillarum strain 1282. | vibrio anguillarum is a gram-negative bacterium associated with vibriosis in atlantic cod (gadus morhua l.). although farmed cod in norway is routinely vaccinated against the infection, outbreaks of v. anguillarum-associated vibriosis still occur. here, we describe the structural characterization of the lps o-chain polysaccharide (o-ps) from atypical isolates of v. anguillarum strain 1282 and show that it is distinct from that previously established for v. anguillarum serotype o2. the structure ... | 2009 | 19476924 |
| molecular cloning and tissue expression patterns of a small conductance calcium-activated potassium channel gene in turbot (scophthalmus maximus l.). | calcium-activated potassium channels on plasma membrane enable potassium influx into the cell with ensuing changes in plasma membrane potential and consequent effects on cellular metabolic functions. recently, this potassium channel was reported to regulate the cellular responses of mammalian immune cells. we have postulated the presence of such a channel in fish immune cells and its potential role in immunoregulation in fish. employing specific primers and rna template, we cloned a segment of a ... | 2009 | 19481607 |
| full-length sequence and expression analysis of a myeloid differentiation factor 88 (myd88) in half-smooth tongue sole cynoglossus semilaevis. | myeloid differentiation factor 88 (myd88) is a universal and crucial adaptor protein, which plays an essential role in the intracellular signalling elicited by il-1r/tlr superfamily. in the present study, we report the full-length sequence of myd88 gene in half-smooth tongue sole (cynoglossus semilaevis). in the 2855 bp genomic sequence, five exons and four introns were identified. the cloned cdna exhibited 110 bp of 5' utr, 576 bp of 3' utr and 858 bp of the entire open-reading frame encoding a ... | 2009 | 19490213 |
| cloning and molecular characterization of two complement bf/c2 genes in large yellow croaker (pseudosciaena crocea). | complement components factor b and c2 are two crucial proteases in the alternative pathway (ap) and classical pathway (cp). two bf/c2 cdnas, lycbf/c2a and lycbf/c2b were isolated from the large yellow croaker (pseudosciaena crocea) by suppression subtractive hybridization (ssh) and rapid amplification of cdna ends (race). through sequence alignment and computer 3d modeling analysis, we found that both of the deduced proteins contain three complement control protein (ccp) modules, a von willebran ... | 2009 | 19490942 |
| mutation of rpos gene decreased resistance to environmental stresses, synthesis of extracellular products and virulence of vibrio anguillarum. | vibrio anguillarum is a gram-negative halophilic bacterium that causes vibriosis in marine fish, freshwater fish and other aquatic animals. bacteria have developed strategies to survive in harsh environments. the alternative sigma factor, rpos (sigma(s)), plays a key role in surviving under stress conditions in some gram-negative bacteria. an rpos mutant of pathogenic v. anguillarum w-1 was constructed by homologous recombination. the sensitivity of the rpos mutant to osmotic stress [2.4 m nacl ... | 2009 | 19527291 |
| characterization of probiotic carnobacteria isolated from rainbow trout (oncorhynchus mykiss) intestine. | to characterize two probiotic carnobacterial isolates, carnobacterium maltaromaticum (b26) and c. divergens (b33), derived from rainbow trout (oncorhynchus mykiss) intestine. | 2008 | 19552776 |
| influence of probiotic feeding duration on disease resistance and immune parameters in rainbow trout. | the effect of feeding the probiotic kocuria sm1 to rainbow trout (oncorhynchus mykiss, walbaum) on disease resistance was evaluated. thus, rainbow trout were fed kocuria sm1 supplemented diets at concentrations of approximately 10(8) cells g(-1) feed for up to four weeks, and then challenged intraperitoneally with vibrio anguillarum at weekly intervals. a two-week feeding regime led to the maximum reduction in mortalities, i.e. 16%, compared to mortalities of 62, 30 and 22% for one, three and fo ... | 2009 | 19555765 |
| a transferrin-like homolog in amphioxus branchiostoma belcheri: identification, expression and functional characterization. | previous studies have shown the universal presence of transferrin (tf) in both invertebrates and vertebrates, but little information is available regarding tf in amphioxus, a protochordate on the evolutionary boundary between invertebrates and vertebrates. here we isolated a tf-like homolog from branchiostoma belcheri, which encodes a deduced protein, bbtfl, of 1256 amino acids containing a n-terminal signal peptide, a conserved transferrin domain in its n-terminal lobe, with a putative iron-bin ... | 2009 | 19577302 |
| in vivo modulation of immune response and antioxidant defense in atlantic cod, gadus morhua following oral administration of oxolinic acid and florfenicol. | oxolinic acid and florfenicol are the commonly used antibiotics for the treatment of bacterial diseases in atlantic cod, gadus morhua. the changes in selected innate humoral immune response of the fish, bacterial proliferation in serum and transcriptional activity of selected immune and antioxidant defense-related genes following oral administration of these antimicrobial compounds were evaluated. juvenile cod (75-100 g) were fed commercial feed coated with either florfenicol (10 mg kg(-1) fish, ... | 2009 | 19596082 |
| identification and molecular characterization of a spätzle-like protein from chinese shrimp (fenneropenaeus chinensis). | in invertebrates, the toll signaling pathway is important for activation of antimicrobial peptides in the innate immune system. activation of the toll pathway requires binding of toll with its ligand spätzle. here we described a spätzle-like protein, designated as fc-spz, from hemocytes of chinese shrimp, fenneropenaeus chinensis. the deduced amino acid sequence of fc-spz shares 54% identity with spätzle-like protein of salmon louse (lepeophtheirus salmonis). semi-quantitative reverse transcript ... | 2009 | 19616633 |
| genetic diversity between two vibrio anguillarum strains exhibiting different virulence by suppression subtractive hybridization. | vibrio anguillarum, a halophilic gram-negative bacterium, is the causative agent of vibriosis in fish. v. anguillarum strain vib72 was defined as having high virulence whereas strain cw1 was defined as having low virulence on the basis of their different ld50 values to zebra fish. suppression subtractive hybridization (ssh) was used to identify genetic differences between these two strains. | 2009 | 19623961 |
| type vi secretion modulates quorum sensing and stress response in vibrio anguillarum. | type vi protein secretion systems (t6ss) are essential for virulence of several gram-negative bacteria. in this study, we identified a t6ss in vibrio anguillarum, a marine bacterium that causes a hemorrhagic septicemia in fish. a partial operon vtsa-h (vibrio type six secretion) was sequenced and shown to encode eight proteins. vtse-h are signature proteins found in other t6sss, while vtsa-d are not associated with t6ss studied so far. in-frame deletions were made in each gene. secretion of a ha ... | 2009 | 19624706 |
| first molluscan tnfr homologue in zhikong scallop: molecular characterization and expression analysis. | tumor necrosis factor receptors (tnfrs) are a superfamily of proteins characterized by the unique cysteine-rich domain (crd) and their important roles in diverse physiological and pathological events such as inflammation, apoptosis, autoimmunity and organogenesis. the first member of the molluscan tnfr family, designated as cftnfr, was identified from zhikong scallop chlamys farreri by expressed sequence tag (est) and rapid amplification of cdna ends (race) approaches. the full-length cdna of cf ... | 2009 | 19632334 |
| a c-type lectin is involved in the innate immune response of chinese white shrimp. | c-type lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this work, a cdna for a new c-type lectin, fclec3, was obtained from chinese white shrimp fenneropenaeus chinensis using expressed sequence tag analysis and rapid amplification of the cdna ends. fclec3 contains an n-terminal signal peptide and a carbohydrate recognition domain (crd). rt-pcr analysis showed that fclec3 was mainly expressed in hepatopancreas and that the expression ... | 2009 | 19647083 |
| the involvement of suppressors of cytokine signaling 2 (socs2) in immune defense responses of chinese mitten crab eriocheir sinensis. | the suppressors of cytokine signaling 2 (socs2) has been identified as negative feedback inhibitors for various cytokines signaling via the jak/stat pathway. in the present studies, the cdna of eriocheir sinensis socs2 (designated as essocs2) was cloned by expressed sequence tag (est) and rapid amplification of cdna ends (race) approaches. the full-length cdna of essocs2 was of 2535bp, consisting of an open reading frame (a orf) of 1071bp encoding a polypeptide of 357 amino acids. the deduced am ... | 2010 | 19686773 |
| a clip domain serine protease (csp) from the chinese mitten crab eriocheir sinensis: cdna characterization and mrna expression. | clip domain serine protease (csp), characterized by conserved clip domains, is a new serine protease family identified mainly in arthropod, and plays important roles in development and immunity. in the present study, the full-length cdna of a csp (designated escsp) was cloned from chinese mitten crab eriocheir sinensis by expressed sequence tags (ests) and pcr techniques. the 1380 bp escsp cdna contained a 1152 bp open reading frame (orf) encoding a putative csp of 383 amino acids, a 5'-untransl ... | 2009 | 19699801 |
| transcriptional differences between triploid and diploid chinook salmon (oncorhynchus tshawytscha) during live vibrio anguillarum challenge. | understanding how organisms function at the level of gene expression is becoming increasingly important for both ecological and evolutionary studies. it is evident that the diversity and complexity of organisms are not dependent solely on their number of genes, but also the variability in gene expression and gene interactions. furthermore, slight differences in transcription control can fundamentally affect the fitness of the organism in a variable environment or during development. in this stud ... | 2010 | 19707232 |
| bacteria associated with sardine (sardina pilchardus) eggs in a natural environment (ría de vigo, galicia, northwestern spain). | abstract the present study was undertaken to describe the epiflora of the eggs of an important fishing species collected in a coastal zone. microflora associated with sardine (sardina pilchardus) eggs collected in the ría de vigo was examined from january to june 2000. the count was carried out in three different ways: a total direct count by epifluorescence, a heterotrophic bacteria count on marine agar (ma) and a total vibrio count on thiosulfate citrate bile sucrose (tcbs). it was observed th ... | 2003 | 19719614 |
| characteristics of na-dependent respiratory chain in vibrio anguillarum, a fish pathogen, in comparison with other marine vibrios. | the activity of membrane-bound nadh oxidase of vibrio anguillarum m93 (serotype j-o-1), which causes vibriosis in freshwater area was activated by na(+) in the same manner as other marine vibrios. however, in addition to na(+), k(+) was also found to positively enhance the nadh oxidase activity of strain m93. this tendency has not been recognized in other marine vibrios. furthermore, the na(+)-dependent nadh oxidase of strain m93 required less na(+) (0.1 m) for its maximum activity than those of ... | 2003 | 19719639 |
| analysis of 16s-23s rrna gene internal transcribed spacer of vibrio anguillarum and vibrio ordalii strains isolated from fish. | the 16s-23s rrna intergenic spacer (its) of vibrio anguillarum and vibrio ordalii were pcr amplified and cloned with ta vector pcr2.1. pcr amplification obtained five products ranging from 917 to 437 bp. three clones were obtained and analysed from all fragments with the exception of 437 bp. these products were designated its-1, its-2, its-3 and its-4. its-1 contained genes for trna(glu(ttc)), trna(lys(ttt)), trna(ala(tgc))and trna(val(tac)), while its-2 was almost the same as the its-1 sequence ... | 2009 | 19732149 |
| the construction of a cdna library enriched for immune genes and the analysis of 7535 ests from chinese mitten crab eriocheir sinensis. | chinese mitten crab eriocheir sinensis is one of the most important aquaculture crustacean species in china. a cdna library was constructed from hemocytes of e. sinensis challenged with the mixture of listonella anguillarum and staphylococcus aureus, and randomly sequenced to collect genomic information and identify genes involved in immune defense response. single-pass 5' sequencing of 10368 clones yielded 7535 high quality ests (expressed sequence tags) and these ests were assembled into 2943 ... | 2009 | 19748587 |
| cloning and molecular characterization of lycc1inh genes in large yellow croaker (pseudosciaena crocea). | complement component 1 inhibitor (c1inh) is a crucial protein in controlling activation of many plasma mediator pathways and can directly interact with gram negative bacteria. the full-length cdna of lycc1inh gene was identified from the large yellow croaker. it is of 2046 nucleotides (nt) encoding a protein of 599 amino acids, with a 5'-untranslated region of 99 nt and a 3'-untranslated region of 147 nt including the poly (a) tail. the deduced protein contains a c-terminal serpin (serine protea ... | 2010 | 19761847 |
| an acyl-coa-binding protein (fcacbp) and a fatty acid binding protein (fcfabp) respond to microbial infection in chinese white shrimp, fenneropenaeus chinensis. | acyl-coa-binding protein (acbp) and fatty acid-binding protein (fabp) are involved in lipid metabolism. acbp plays a key role in multiple cellular tasks including modulation of fatty acid biosynthesis, enzyme regulation, vesicular trafficking, and gene regulation. in our study, a 536 bp cdna of acbp (fcacbp) was cloned and identified as a widely distributed gene in the chinese white shrimp, fenneropenaeus chinensis. its expression in intestine was upregulated in response to white spot syndrome v ... | 2009 | 19766195 |
| a putative endocrine factor sibd (single insulin binding domain protein) involved in immune response of chinese mitten crab eriocheir sinensis. | insulin growth factor binding proteins (igfbps), characterized by the conserved insulin binding (ib) domains, are important components of endocrine system and play key roles in metabolism and growth. in the present study, the full-length cdna of a single ib domain protein (designated essibd) was identified from chinese mitten crab eriocheir sinensis based on expressed sequence tag (est) sequence. the 1187 bp essibd cdna contained a 321 bp open reading frame (orf) encoding a putative protein of 1 ... | 2010 | 19772924 |
| ai-2 quorum-sensing inhibitors affect the starvation response and reduce virulence in several vibrio species, most likely by interfering with luxpq. | the increase of disease outbreaks caused by vibrio species in aquatic organisms as well as in humans, together with the emergence of antibiotic resistance in vibrio species, has led to a growing interest in alternative disease control measures. quorum sensing (qs) is a mechanism for regulating microbial gene expression in a cell density-dependent way. while there is good evidence for the involvement of auto-inducer 2 (ai-2)-based interspecies qs in the control of virulence in multiple vibrio spe ... | 2009 | 19778962 |
| inhibitory activity of phaeobacter strains against aquaculture pathogenic bacteria. | a total of 523 bacterial strains were isolated during a 4-year period from mollusc hatcheries (flat oyster and clams) in galicia (nw spain). all of the strains were tested for their antibacterial activity against three larval pathogens (vibrio anguillarum usc-72, v. neptunius pp-145.98, and vibrio sp. pp-203). of the isolates, 52 inhibited at least one of the target strains, and 11 inhibited all of them. the main source of active strains was oyster larvae, followed by water, tank surfaces, spat, ... | 2009 | 19784930 |
| antibacterial activity of marine culturable bacteria collected from a global sampling of ocean surface waters and surface swabs of marine organisms. | the purpose of the present study was to isolate marine culturable bacteria with antibacterial activity and hence a potential biotechnological use. seawater samples (244) and 309 swab samples from biotic or abiotic surfaces were collected on a global danish marine research expedition (galathea 3). total cell counts at the seawater surface were 5 x 10(5) to 10(6) cells/ml, of which 0.1-0.2% were culturable on dilute marine agar (20 degrees c). three percent of the colonies cultured from seawater i ... | 2010 | 19823914 |
| responses of atlantic cod gadus morhua head kidney leukocytes to phytase produced by gastrointestinal-derived bacteria. | this study identified phytase-producing bacteria that were previously isolated from the gastrointestinal tract of atlantic cod, gadus morhua and determined its effect on head kidney leukocytes. out of the 216 bacterial strains tested, the two phytase producers were identified as pseudomonas sp. and psychrobacter sp. based on their 16s rdna sequence. crude phytase from these two bacterial strains was produced employing the shake flask method. even though the total protein of the crude phytase was ... | 2010 | 19844802 |
| [characterization of listonella anguillarum as the aetiological agent of vibriosis occurred in cultured ayu (plecoglossus altivelis) in ninghai country, china]. | ayu (plecoglossus altivelis) vibriosis threatens ayu aquaculture seriously caused by mass mortality due to severe infections. we characterized the vibriosis pathogen of ayu in ninghai country. | 2009 | 19873758 |
| kocuria sm1 controls vibriosis in rainbow trout (oncorhynchus mykiss, walbaum). | to develop probiotics for the control of vibriosis caused by vibrio anguillarum and vibrio ordalii in finfish. | 2010 | 19929950 |
| an immune responsive multidomain galectin from bay scallop argopectens irradians. | galectins are a family of beta-galactoside-binding lectins which play crucial roles in innate immunity of vertebrates and invertebrates. in the present study, the cdna of a galectin with multiple carbohydrate-recognition domains (crds) was cloned from bay scallop argopectens irradians (designated aigal1) by expressed sequence tag (est) and rapid amplification of cdna ends (race) techniques. the full-length cdna of aigal1 was of 2235 nucleotides, encoding a polypeptide of 549 amino acids. smart p ... | 2010 | 19944171 |
| identification and characterization of an intracellular cu, zn-superoxide dismutase (iccu/zn-sod) gene from clam venerupis philippinarum. | superoxide dismutase (sod, ec 1.15.1.1) represents one kind of enzyme involved in scavenging the high level of reactive oxygen species (ros) into molecular oxygen and hydrogen peroxide. in the present study, the intracellular cu/zn-sod gene (iccu/zn-sod) of venerupis philippinarum (denoted as vpsod) was identified from haemocytes by homology cloning and racr approaches. the full-length cdna of vpsod consisted of 910 nucleotides with a canonical polyadenylation signal sequence aataaa, a polya tai ... | 2010 | 19948222 |
| a novel multivalent vaccine based on secretary antigen-delivery induces protective immunity against vibrio anguillarum and aeromonas hydrophila. | in our previous work, four secretory antigen-delivery systems based on different signal peptides (sps) (sp(hlya), sp(rtxa), sp(vah3) and sp(empa)) have been successfully established in attenuated vibrio anguillarum. to investigate the potential application of the antigen-delivery systems in v. anguillarum multivalent vector vaccine, the glyceraldehyde-3-phosphate dehydrogenase (gapdh) from pathogenic aeromonas hydrophila, as a putative protective antigen, was fused to the four delivery systems, ... | 2010 | 20026209 |
| gene cloning and characterization of ferritin h and m subunits from large yellow croaker (pseudosciaena crocea). | ferritin, an iron-binding protein, plays a role in host immune response to invading stimulus. two ferritin subunit cdnas, lycfh and lycfm, were identified from large yellow croaker, pseudosciaena crocea. analysis of the lycfh and lycfm 5'-utr showed that both had a putative iron responsive element (ire). amino acid sequence alignment and phylogenetic analysis indicated that lycfh and lycfm had high identity to h and m type subunit of vertebrate ferritins respectively, while both of them showed t ... | 2010 | 20034577 |
| molecular cloning and characterization of three crustins from the chinese white shrimp, fenneropenaeus chinensis. | antibacterial peptides crustins are the effector molecules of innate immunity in decapods. in this study, three crustin cdna sequences (fc-crus 1, fc-crus 2, and fc-crus 3) were cloned from the chinese white shrimp fenneropenaeus chinensis. the full-length cdnas of fc-crus 2 and 3 are 473 bp and 574 bp, respectively. the deduced peptides of fc-crus 2 and 3 contain a signal peptide and a crustin domain at the c-terminal formed by twelve conserved cysteine residues. the partial sequence of fc-cru ... | 2010 | 20036745 |
| polymorphism of the superoxide dismutase gene family in the bay scallop (argopecten irradians) and its association with resistance/susceptibility to vibrio anguillarum. | the superoxide dismutases (sods) are a family of enzymes that function as the first line of antioxidant defense against highly reactive superoxide radicals. the bay scallop argopecten irradians contains three unique superoxide dismutases: ai-iccuznsod, ai-mnsod and ai-eccuznsod, which were characterized in our previous studies. qrt-pcr was also performed to characterize the temporal expression of sods in the hemocytes of bay scallops injected with the bacterium vibrio anguillarum. to characteriz ... | 2010 | 20045025 |
| aictl-6, a novel c-type lectin from bay scallop argopecten irradians with a long c-type lectin-like domain. | c-type lectins are a superfamily of carbohydrate-recognition proteins which play crucial roles in the innate immunity. in this study, a novel c-type lectin gene from scallop argopecten irradians (designated as aictl-6) was cloned by rapid amplification of cdna ends (race) approach based on expression sequence tag (est) analysis. the full-length cdna of aictl-6 was 1080 bp. the open reading frame encoded a polypeptide of 307 amino acids, including a signal sequence and a c-type lectin-like domain ... | 2011 | 20045059 |
| concurrent injection of a rhabdovirus-specific dna vaccine with a polyvalent, oil-adjuvanted vaccine delays the specific anti-viral immune response in atlantic salmon, salmo salar l. | vaccines are commonly used in salmonid aquaculture as a method of disease prevention. although there is a substantial amount of published research regarding the immunological and physiological effects following the injection of different polyvalent vaccines and dna vaccines, there are no published reports examining the physiological and immunological effects of concurrent vaccine injection, which is the situation encountered in aquaculture. using key immunological parameters such as lysozyme act ... | 2010 | 20045062 |
| expression of mytilus immune genes in response to experimental challenges varied according to the site of collection. | mussels live in diverse coastal environments experience various physical, chemical and biological conditions, which they counteract with functional adjustments and heritable adaptive changes. in order to investigate possible differences in immune system capabilities, we analyzed by qpcr the expression levels of 4 immune genes (defensin, mytilin b, myticin b, lysozyme) and hsp70 in the mediterranean mussel, mytilus galloprovincialis collected in 3 european farming areas {atlantic ocean-ría de vig ... | 2010 | 20045066 |