| pantoea agglomerans liver abscess in a resident of brazilian amazonia. | | 2009 | 20306748 |
| [isolation and characterization of a facultative anaerobe pantoea agglomerans mfc-3 and its humic substance-and fe(iii) - respiring activity]. | a strain of humic substance- and fe(iii)- reducing bacterium was isolated from the subterranean forest sediment and designated as mfc-3. the strain is facultative anaerobic, gram-negative, motile and rod (1.0-3.0 microm long, 0.5-1.0 microm wide) and identified as pantoea agglomerans with the 16s rdna sequence analyses. batch experiments were conducted to investigate its humic substance-and fe(iii)-respiring activity. the results showed that mfc-3 was capable of anaerobic respiration on anthraqu ... | 2010 | 20329545 |
| isolation of some pathogenic bacteria from the great spruce bark beetle, dendroctonus micans and its specific predator, rhizophagus grandis. | some bacteria were isolated from dendroctonus micans and its specific predator, rhizophagus grandis. six bacteria from d. micans were identified as bacillus pumilus, enterobacter intermedius, citrobacter freundii, cellulomonas flavigena, microbacterium liquefaciens and enterobacter amnigenus, three bacteria from r. grandis as klebsiella pneumoniae, pantoea agglomerans and serratia grimesii, on the basis of fatty acid methyl ester analysis and carbon utilization profile by using microbial identif ... | 2010 | 20336502 |
| genome sequence of pantoea ananatis lmg20103, the causative agent of eucalyptus blight and dieback. | pantoea ananatis is a gram-negative plant pathogen that causes disease on a broad range of host plants, including pineapple, maize, rice, onion, melons, and eucalyptus, and has been implicated in several cases of human disease. here, we report the genome sequence of p. ananatis lmg20103 isolated from diseased eucalyptus in south africa. | 2010 | 20348253 |
| diversity of fecal coliforms and their antimicrobial resistance patterns in wastewater treatment model plant. | the occurrence of resistance patterns among wastewater fecal coliforms was determined in the study. susceptibility of the isolates was tested against 19 antimicrobial agents: aminoglycosides, aztreonam, carbapenems, cephalosporines, beta-lactam/beta-lactamase inhibitors, penicillines, tetracycline, trimethoprim/sulfamethoxazole, and fluoroquinolones. additionally the removal of resistant isolates was evaluated in the laboratory-scale wastewater treatment model plant (m-wwtp), continuously suppli ... | 2010 | 20351416 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| assessing the impact of fungicide enostroburin application on bacterial community in wheat phyllosphere. | fungicides have been used extensively for controlling fungal pathogens of plants. however, little is known regarding the effects that fungicides upon the indigenous bacterial communities within the plant phyllosphere. the aims of this study were to assess the impact of fungicide enostroburin upon bacterial communities in wheat phyllosphere. culture-independent methodologies of 16s rdna clone library and 16s rdna directed polymerase chain reaction with denaturing gradient gel electrophoresis (pcr ... | 2010 | 20397397 |
| culture-dependent and culture-independent characterization of microorganisms associated with aedes aegypti (diptera: culicidae) (l.) and dynamics of bacterial colonization in the midgut. | in this work we show that the lumen of aedes aegypti midgut is highly colonized by bacteria that were identified by culture-dependent and culture-independent methods. rdna sequences obtained were compared with those from genbank and the main bacterial genera identified were: serratia, klebsiella, asaia, bacillus, enterococcus, enterobacter,kluyvera and pantoea. all genera were identified in midgut except enterobacter that was observed only in eggs. asaia and pantoea were also identified in eggs ... | 2010 | 20434424 |
| application of whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry for rapid identification and clustering analysis of pantoea species. | pantoea agglomerans is an ecologically diverse taxon that includes commercially important plant-beneficial strains and opportunistic clinical isolates. standard biochemical identification methods in diagnostic laboratories were repeatedly shown to run into false-positive identifications of p. agglomerans, a fact which is also reflected by the high number of 16s rrna gene sequences in public databases that are incorrectly assigned to this species. more reliable methods for rapid identification ar ... | 2010 | 20453125 |
| genomic and phenotypic characterization of a nonpigmented variant of pantoea vagans biocontrol strain c9-1 lacking the 530-kb megaplasmid ppag3. | a 530-kb megaplasmid ppag3 contributing 10.8% of the total genome of pantoea vagans biocontrol strain c9-1 was sequenced. a rare nonpigmented variant c9-1w was obtained and shown to have lost ppag3, but retained all other plasmids (ppag1, ppag2). phenotypic characterization of the variant confirmed the function of several annotated genes that may influence ecological fitness and efficacy. metabolic profiling revealed important plasmid-based carbon utilization phenotypes. plasmid loss resulted in ... | 2010 | 20487014 |
| a sequence that affects the copy number and stability of psw200 and cole1. | pantoea stewartii sw2 contains 13 plasmids. one of these plasmids, psw200, has a replicon that resembles that of cole1. this study demonstrates that psw200 contains a 9-bp up element, 5'-aagatcttc, which is located immediately upstream of the -35 box in the rnaii promoter. a transcriptional fusion study reveals that substituting this 9-bp sequence reduces the activity of the rnaii promoter by 78%. the same mutation also reduced the number of plasmid copies from 13 to 5, as well as the plasmid st ... | 2010 | 20494993 |
| pantoea allii sp. nov., isolated from onion plants and seed. | eight yellow-pigmented, gram-negative, rod-shaped, oxidase-negative, motile, facultatively anaerobic bacteria were isolated from onion seed in south africa and from an onion plant exhibiting centre rot symptoms in the usa. the isolates were assigned to the genus pantoea on the basis of phenotypic and biochemical tests. 16s rrna gene sequence analysis and multilocus sequence analysis (mlsa), based on gyrb, rpob, infb and atpd sequences, confirmed the allocation of the isolates to the genus pantoe ... | 2011 | 20495023 |
| structure of the d-alanylgriseoluteic acid biosynthetic protein ehpf, an atypical member of the anl superfamily of adenylating enzymes. | the structure of ehpf, a 41 kda protein that functions in the biosynthetic pathway leading to the broad-spectrum antimicrobial compound d-alanylgriseoluteic acid (aga), is reported. a cluster of approximately 16 genes, including ehpf, located on a 200 kbp plasmid native to certain strains of pantoea agglomerans encodes the proteins that are required for the conversion of chorismic acid to aga. phenazine-1,6-dicarboxylate has been identified as an intermediate in aga biosynthesis and deletion of ... | 2010 | 20516619 |
| hydrocarbon degradation and plant colonization by selected bacterial strains isolated from italian ryegrass and birdsfoot trefoil. | to assess the degradation potential and plant colonization capacity of four alkane-degrading strains (itsi10, itri15, itrh76 and btrh79) in combination with birdsfoot trefoil and italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere. | 2010 | 20522148 |
| redesign, reconstruction, and directed extension of the brevibacterium linens c40 carotenoid pathway in escherichia coli. | in this study, the carotenoid biosynthetic pathways of brevibacterium linens dsmz 20426 were reconstructed, redesigned, and extended with additional carotenoid-modifying enzymes of other sources in a heterologous host escherichia coli. the modular lycopene pathway synthesized an unexpected carotenoid structure, 3,4-didehydrolycopene, as well as lycopene. extension of the novel 3,4-didehydrolycopene pathway with the mutant pantoea lycopene cyclase crty(2) and the rhodobacter spheroidene monooxyge ... | 2010 | 20525861 |
| comparison of specificity and sensitivity of immunochemical and molecular techniques for determination of clavibacter michiganensis subsp. michiganensis. | detection of clavibacter michiganensis subsp. michiganensis (cmm), causing bacterial canker of tomato, was verified using pta-elisa and ifas with pabs of neogen europe ltd. (uk), and with published and also laboratory-generated pcr primers from the cmm tomatinase gene. the specificity of this technique was determined with 15 plant-pathogenic and 4 common, saprophytic bacteria. with ifas, crossreactions were found for pantoea dispersa, p. agglomerans and rahnella aquatilis, and with pta-elisa for ... | 2010 | 20526836 |
| enrichment of carotenoids in flaxseed by introducing a bacterial phytoene synthase gene. | carotenoids are well-known natural pigments, typically ranging from yellow to red. carotenoids are industrially utilized as functional materials due to their strong antioxidant properties. phytoene synthesis is known to be a rate-determining step in the entire carotenoid biosynthetic pathway in plants. we show methods of pathway engineering for the enrichment of carotenoids in flaxseed (linseed; linum usitatissimum l.), which is an industrially important oleaginous crop. a phytoene synthase gene ... | 2010 | 20552453 |
| effect of pine bark and compost on the biological denitrification process of non-hazardous landfill leachate: focus on the microbiology. | in an attempt to optimize the cost-efficiency of landfill leachate treatment by biological denitrification process, our study focused on finding low-cost alternatives to traditional expensive chemicals such as composted garden refuse and pine bark, which are both available in large amount in south african landfill sites. the overall objective was to assess the behaviour of the bacterial community in relation to each substrate while treating high strength landfill leachates. denitrification proce ... | 2010 | 20554377 |
| diversity of the total bacterial community associated with ghanaian and brazilian cocoa bean fermentation samples as revealed by a 16 s rrna gene clone library. | cocoa bean fermentation is a spontaneous process involving a succession of microbial activities, starting with yeasts, followed by lactic acid bacteria and acetic acid bacteria. so far, all microbiological studies about cocoa bean fermentation were based on culture-dependent (isolation, cultivation, and identification), or, more recently, culture-independent (pcr-dgge, or polymerase chain reaction denaturing gradient gel electrophoresis) methods. using a metagenomic approach, total dna was extra ... | 2010 | 20559826 |
| pthg from pantoea agglomerans pv. gypsophilae encodes an avirulence effector that determines incompatibility in multiple beet species. | summary pantoea agglomerans pv. gypsophilae (pag) causes root and crown gall disease on gypsophila, whereas p. agglomerans pv. betae (pab) induces the disease on beet as well as gypsophila. both pathovars harbour a pathogenicity plasmid (ppath(pag) or ppath(pab)) that determines disease development. we have previously isolated and partially characterized a pleiotropic gene from the ppath(pag), designated as pthg, that encodes a virulence factor in gypsophila and an elicitor of a hypersensitive-l ... | 2004 | 20565587 |
| pantoea agglomerans pvs. gypsophilae and betae, recently evolved pathogens? | summary pantoea agglomerans pvs. gypsophilae and betae taxonomy: bacteria; proteobacteria; gamma subdivision; order enterobacteriales; family enterobacteriaceae; species pantoea agglomerans. microbiological properties: gram-negative, non-capsulated, non-spore-forming, predominately motile rode. disease symptoms: gall formation at wound sites, mainly in the crown region of the stem. the host range of p. agglomerans pv. gypsophilae is restricted to gypsophila paniculata, whereas p. agglomerans pv. ... | 2003 | 20569391 |
| erwinia amylovora: the molecular basis of fireblight disease. | summary taxonomy: bacteria; proteobacteria; gamma subdivision; order enterobacteriales; family enterobacteriaceae; genus erwinia. microbiological properties: gram-negative, motile rods. related species:e. carotovora (soft-rot diseases), e. chrysanthemi (soft-rot diseases), e. (pantoea) stewartii (stewart's wilt of corn), e. (pantoea) herbicola (epiphyte). host range: affects rosaceous plants, primarily members of the pomoideae. economically important hosts are apple and pear. the commercial impl ... | 2000 | 20572979 |
| the eye fly siphunculina funicola (diptera: chloropidae) as a carrier of pathogenic bacteria in thailand. | the oriental eye fly siphunculina funicola (1.0-1.6 mm) is extremely annoying to humans and domestic animals, feeding on mucous membranes, secretions, wounds, eyes, and other moist surfaces of the host body. in many rural areas of thailand heavy populations of this fly prevail where they aggregate on a variety of hanging substrates, such as strings, nest trailings, electrical lines, decorations, ropes, cob webs, clothes hangers, automobile radio antennae and other items in open shade close to th ... | 2010 | 20578483 |
| carob pulp as raw material for production of the biocontrol agent p. agglomerans pbc-1. | large-scale production has been the major obstacle to the success of many biopesticides. the spreading of microbial biocontrol agents against postharvest disease, as a safe and environmentally friendly alternative to synthetic fungicides, is quite dependent on their industrial mass production from low-cost raw materials. considerable interest has been shown in using agricultural waste products and by-products from food industry as nitrogen and carbon sources. in this work, carob pulp aqueous ext ... | 2010 | 20593299 |
| gene expression profile of the plant pathogen fusarium graminearum under the antagonistic effect of pantoea agglomerans. | the pathogenic fungus fusarium graminearum is an ongoing threat to agriculture, causing losses in grain yield and quality in diverse crops. substantial progress has been made in the identification of genes involved in the suppression of phytopathogens by antagonistic microorganisms; however, limited information regarding responses of plant pathogens to these biocontrol agents is available. gene expression analysis was used to identify differentially expressed transcripts of the fungal plant path ... | 2010 | 20623455 |
| 1,3-propanediol production potential of clostridium saccharobutylicum nrrl b-643. | owing to the significant interest in biofuel production in the form of biodiesel, vast amount of glycerol as a waste product is produced all over the world. among the economically viable and ecologically acceptable solutions for the safe disposal of this waste, biotechnological conversion of glycerol into a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) seems to be very promising. in this study, 1,3-pdo production potential of clostridium saccharobutylicum nrrl b-643 was stud ... | 2010 | 20647065 |
| application of two bicistronic systems involving 2a and ires sequences to the biosynthesis of carotenoids in rice endosperm. | coordination of multiple transgenes is essential for metabolic engineering of biosynthetic pathways. here, we report the utilization of two bicistronic systems involving the 2a sequence from the foot-and-mouth disease virus and the internal ribosome entry site (ires) sequence from the crucifer-infecting tobamovirus to the biosynthesis of carotenoids in rice endosperm. two carotenoid biosynthetic genes, phytoene synthase (psy) from capsicum and carotene desaturase (crti) from pantoea, were linked ... | 2010 | 20649940 |
| protective effects of the immunopotentiator from pantoea agglomerans 1 on chemotherapeutic agent-induced macrophage growth inhibition. | the immunopotentiator from pantoea agglomerans 1 (ip-pa1) is an edible lipopolysaccharide (lps) derived from symbiotic bacteria found in crops. ip-pa1 is known to ameliorate chemotherapy-induced immunosuppression; therefore, its macrophage-activating effect in the presence of chemotherapeutic agents was evaluated. | 2010 | 20651348 |
| identification of the corn pathogen pantoea stewartii by mass spectrometry of whole-cell extracts and its detection with novel pcr primers. | pantoea stewartii subsp. stewartii is the causative agent of stewart's wilt, a bacterial disease transmitted by the corn flea beetle mainly to sweet corn (zea mays). in many countries, it is classified as a quarantine organism and must be differentiated from other yellow enteric bacteria frequently occurring with corn. we have created novel primers from the psts-glms region of p. stewartii for use in conventional pcr (cpcr) and quantitative pcr (qpcr). to facilitate rapid diagnosis, we applied m ... | 2010 | 20656863 |
| sporadic pantoea agglomerans bacteremia in a near-term female: case report and review of literature. | pantoea agglomerans is a plant pathogen and an unusual cause of human disease typically associated with thorn prick injuries or contaminated parenteral fluids. in the neonate, p. agglomerans has been reported to cause bacteremia or sepsis 17 times previously. in most of these cases, the source of infection has been contaminated parenteral nutrition or has been associated with indwelling catheters. we present a rare case of p. agglomerans bacteremia in a 35-week-female born via vaginal delivery c ... | 2010 | 20657073 |
| culturable bacterial microflora associated with nectarine fruit and their potential for control of brown rot. | microflora of fruit surfaces have been the best source of antagonists against fungi causing postharvest decay of fruit. however, there is little information on microflora colonizing surfaces of fruits other than grape, apple, and citrus. we characterized bacterial microflora on nectarine fruit surfaces from the early stage of development until harvest. identification of bacterial strains was made using midi (fatty acid methyl ester analysis) and biolog systems. biolog identified 35% and midi 53% ... | 2010 | 20657618 |
| isolation and characterization of folate-producing bacteria from oat bran and rye flakes. | the aim of this research was to identify endogenous bacteria in commercial oat bran and rye flake products in order to study their folate production capability while maintaining the soluble dietary fibre components in physiologically active, unhydrolyzed form. fourty-two bacteria were isolated from three different oat bran products and 26 bacteria from one rye flake consumer product. the bacteria were tentatively identified by sequence analysis of the 16s rrna genes. the identification results r ... | 2010 | 20678822 |
| [biodiversity of phosphate-dissolving and plant growth--promoting endophytic bacteria of two crops]. | we isolated and characterized phosphate-dissolving endophytic bacteria from two commonly cultivated crops. | 2010 | 20687333 |
| anaerobic biotransformation of high concentrations of chloroform by an enrichment culture and two bacterial isolates. | a fermentative enrichment culture (designated dhm-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (cf) to nontoxic end products. two pantoea spp. were isolated from dhm-1 that also possess this dechlorination capability. following acclimation to increasing levels of cf, corn syrup-grown dhm-1 was able to transform over 500 mg/liter cf in the presence of vitamin b(12) (approximately 3% of cf on a molar basis) at a rate as high as 22 mg/liter/d ... | 2010 | 20693443 |
| production of folate by bacteria isolated from oat bran. | twenty bacteria isolated from three commercial oat bran products were tested for their folate production capability. the bacteria as well as some reference organisms were grown until early stationary phase on a rich medium (ypd), and the amount of total folate in the separated cell mass and the culture medium (supernatant) was determined by microbiological assay. folate vitamer distribution was determined for eight bacteria including one isolated from rye flakes. for seven bacteria the effect of ... | 2010 | 20708290 |
| a case of bilateral endogenous pantoea agglomerans endophthalmitis with interstitial lung disease. | we here in report a case of bilateral endogenous endophthalmitis caused by pantoea agglomerans (p. agglomerans) in a patient who had interstitial lung disease and was treated with oral corticosteroids. a 72-year-old man presented with decreased visual acuity in both eyes nine days after he received oral corticosteroids. he had marked uveitis, cataracts, and vitreous opacities. cultures were taken of blood, aqueous humor, and vitreous. we initially suspected a fungal etiology and treated him with ... | 2010 | 20714391 |
| detection of airborne bacteria in a german turkey house by cultivation-based and molecular methods. | today's large-scale poultry production with densely stocked and enclosed production buildings is often accompanied by very high concentrations of airborne microorganisms leading to a clear health hazard for employees working in such environments. depending on the expected exposure to microorganisms, work has to be performed under occupational safety conditions. in this study, turkey houses bioaerosols were investigated by cultivation-based and molecular methods in parallel to determine the conce ... | 2010 | 20720091 |
| [absence of mutagenic effect of pseudomonas syringae pv. atrofaciens 9400 and pantoea agglomerans p324 culture liquids]. | the mutagenic activity of the culture liquids of phytopathogenic strain pseudomonas syringae pv. atrofaciens 9400 and epiphytic strain pantoea agglomerans p324 was studied in the ames test and allium cepa-test. in pro- and eucariotic test-systems no effect of the culture liquids of these bacteria on spontaneous mutations of salmonella typhimurium ta98 and ta100 or chromosome aberrations in the cells of allium cepa root apical meristem was found. | 2010 | 20812509 |
| mechanistically compatible mixtures of bacterial antagonists improve biological control of fire blight of pear. | mixtures of biological control agents can be superior to individual agents in suppressing plant disease, providing enhanced efficacy and reliability from field to field relative to single biocontrol strains. nonetheless, the efficacy of combinations of pseudomonas fluorescens a506, a commercial biological control agent for fire blight of pear, and pantoea vagans strain c9-1 or pantoea agglomerans strain eh252 rarely exceeds that of individual strains. a506 suppresses growth of the pathogen on fl ... | 2011 | 20839962 |
| control of fire blight by pseudomonas fluorescens a506 and pantoea vagans c9-1 applied as single strains and mixed inocula. | the biological control agents pseudomonas fluorescens a506 and pantoea vagans c9-1 were evaluated individually and in combination for the suppression of fire blight of pear or apple in 10 field trials inoculated with the pathogen erwinia amylovora. the formulation of pathogen inoculum applied to blossoms influenced establishment of the pathogen and the efficacy of biological control. pantoea vagans c9-1 suppressed fire blight in all five trials in which the pathogen was applied as lyophilized ce ... | 2010 | 20839963 |
| arsenic mobilization by epilithic bacterial communities associated with volcanic rocks from camarones river, atacama desert, northern chile. | the arsenic biogeochemical cycle is greatly dependent on microbial transformations that affect both the distribution and mobility of arsenic species in the environment. in this study, a microbial biofilm from volcanic rocks was characterized on the basis of its bacterial composition and ability to mobilize arsenic under circumneutral ph. biofilm microstructure was analyzed by scanning electron microscopy (sem)-energy-dispersive spectroscopy (eds). strains were isolated from biofilms and identifi ... | 2010 | 20859623 |
| recovery from immunosuppression-related disorders in humans and animals by ip-pa1, an edible lipopolysaccharide. | immunopotentiator from pantoea agglomerans 1 (ip-pa1), an edible lipopolysaccharide (lps) derived from symbiotic bacteria in crops, is a promising immunomodulator. it activates macrophages and protects from chemotherapeutic agent-induced growth inhibition in macrophages in vitro. we showed the immune-recovery effects of ip-pa1 in a chicken model of dexamethasone-induced stress in which ip-pa1 inhibited thymic and bursal atrophy and improved antibody production in response to vaccination. further ... | 2010 | 20871028 |
| preparation of lipopolysaccharide derived from pantoea agglomerans labeled with fluorescence as a tracer for kinetics analysis. | intradermal and/or oral administration of lipopolysaccharides derived from pantoea agglomerans (ip-pa1) have shown multiple positive effects such as phylactic, anti-allergic and anti-tumor effects. it has been reported that the effects of ip-pa1 were derived from the induction of activated macrophages. however, it has not been actually clarified whether or not the orally administered ip-pa1 absorbed in the intestine reached and activated tissue macrophages. the aim of this study was to prepare a ... | 2010 | 20871034 |
| high versus low level expression of the lycopene biosynthesis genes from pantoea ananatis in escherichia coli. | the heterologous synthesis of lycopene in non-carotenogenic escherichia coli required the introduction of the biosynthesis genes crte, crtb, and crti. recombinant e. coli strains, expressing each lycopene biosynthesis gene from pantoea ananatis using multi-copy plasmid or single-copies after stable chromosomal integration, were cultivated and the formation of lycopene was investigated. the different expression conditions significantly influenced the lycopene formation as well as the growth behav ... | 2010 | 20882315 |
| the nonribosomal peptide synthetase enzyme ddad tethers n(β)-fumaramoyl-l-2,3-diaminopropionate for fe(ii)/α-ketoglutarate-dependent epoxidation by ddac during dapdiamide antibiotic biosynthesis. | the gene cluster from pantoea agglomerans responsible for biosynthesis of the dapdiamide antibiotics encodes an adenylation-thiolation didomain protein, ddad, and an fe(ii)/α-ketoglutarate-dependent dioxygenase homologue, ddac. here we show that ddad, a nonribosomal peptide synthetase module, activates and sequesters n(β)-fumaramoyl-l-2,3-diaminopropionate as a covalently tethered thioester for subsequent oxidative modification of the fumaramoyl group. ddac catalyzes fe(ii)- and α-ketoglutarate- ... | 2010 | 20945916 |
| genome sequence of the biocontrol agent pantoea vagans strain c9-1. | pantoea vagans is a gram-negative enterobacterial plant epiphyte of a broad range of plants. here we report the 4.89-mb genome sequence of p. vagans strain c9-1 (formerly pantoea agglomerans), which is commercially registered for biological control of fire blight, a disease of pear and apple trees caused by erwinia amylovora. | 2010 | 20952567 |
| bacterial community associated with the trunk latex of hancornia speciosa gomes (apocynaceae) grown in the northeast of brazil. | prevention or cure of different illnesses through the use of plant latex is a worldwide known concept. the antifungal activity of hancornia speciosa latex has been observed against candida albicans. however, h. speciosa latex is not a sterile plant exudate and secondary metabolites produced by bacteria could be involved in fungal inhibition. in the present study, the bacterial communities of the latex from three h. speciosa trees were characterized using traditional plating and molecular methods ... | 2010 | 20960060 |
| survival of phosphate-solubilizing bacteria against dna damaging agents. | phosphate-solubilizing bacteria (psbs) were isolated from different plant rhizosphere soils of various agroecological regions of india. these isolates showed synthesis of pyrroloquinoline quinone (pqq), production of gluconic acid, and release of phosphorus from insoluble tricalcium phosphate. the bacterial isolates synthesizing pqq also showed higher tolerance to ultraviolet c radiation and mitomycin c as compared to escherichia coli but were less tolerant than deinococcus radiodurans. unlike e ... | 2010 | 20962905 |
| isolation of phosphate solubilizing bacteria and their potential for lead immobilization in soil. | lead (pb), a highly toxic heavy metal forms stable compounds with phosphate (p). the potential of phosphate solubilizing bacteria (psb) to immobilize pb by enhancing solubilization of insoluble p compounds was tested in this research. eighteen different psb strains isolated from p amended and pb contaminated soils were screened for their efficiency in p solubilization. the psb isolated from p amended soils solubilized 217-479 mg/l of p while the psb from pb contaminated soil solubilized 31-293 m ... | 2010 | 20971555 |
| new blue pigment produced by pantoea agglomerans and its production characteristics at various temperatures. | a bacterium capable of producing a deep blue pigment was isolated from the environment and identified as pantoea agglomerans. the pigment production characteristics of the bacterium under various conditions were studied. the optimal agar plate ingredients for pigment production by the bacterium were first studied: the optimal ingredients were 5 g/liter glucose, 10 g/liter tryptic soy broth, and 40 g/liter glycerol at ph 6.4. bacterial cells grew on the agar plate during the incubation, while the ... | 2010 | 20971865 |
| biosynthesis of zeaxanthin in recombinant pseudomonas putida. | pseudomonas putida kt2440 strain was investigated for biosynthesis of the valuable xanthophyll zeaxanthin. a new plasmid was constructed harboring five carotenogenic genes from pantoea ananatis and three genes from escherichia coli under control of an l: -rhamnose-inducible promoter. pseudomonas putida kt2440 wild type hardly tolerated the plasmids for carotenoid production. mating experiments with e. coli s17-1 strains revealed that the carotenoid products are toxic to the pseudomonas putida ce ... | 2010 | 21038098 |
| the bacterial community in 'taberna' a traditional beverage of southern mexico. | to characterize the bacterial community of taberna, an alcoholic traditional beverage from the southern part of mexico produced by the fermentation of the coyol palm sap (acrocomia aculeate). | 2010 | 21039665 |
| a case of pantoea endophthalmitis. | a previously healthy 50-year-old man was transferred to our hospital for evaluation of acute inflammation in his right eye after ocular trauma while using a grass mower. slit lamp examination showed 1 mm-length full thickness corneal laceration without leakage, 4+ cells and inflammatory membrane in the anterior chamber, 10% hypopyon, posterior synechiae formation, and cataract change. upon orbital computerized tomography, a metallic intraocular foreign body in the lens was identified. vitrectomy ... | 2010 | 21052515 |
| effect of transgenic rhizobacteria overexpressing citrobacter braakii appa on phytate-p availability to mung bean plants. | rhizosphere microorganisms possessing phytase activity are considered important for rendering phytate-p available to plants. in the present study, citrobacter braakii phytase gene (appa) was over-expressed in rhizobacteria possessing plant growth promoting (pgp) traits for increasing their potential as bioinoculants. appa was cloned under the lac promoter in the broad host-range expression vector pbbr1mcs2. transformation of the recombinant construct pcbappa resulted in high constitutive phytase ... | 2010 | 21124052 |
| dynamics, diversity and function of endophytic siderophore-producing bacteria in rice. | siderophore production confers to bacteria competitive advantages to colonize plant tissues and to exclude other microorganisms from the same ecological niche. this work shows that the community of endophytic siderophore-producing bacteria (spb) associated to oryza sativa cultivated in uruguayan soils is dynamic and diverse. these bacteria were present in grains, roots, and leaves, and their density fluctuated between log(10) 3.44 and log(10) 5.52 cfu g(-1) fresh weight (fw) during the plant gro ... | 2010 | 21128071 |
| paenibacillus polymyxa jb05-01-1 and its perspectives for food conservation and medical applications. | the aim of this study was to isolate a novel bacterial strain with strong and broad spectrum antibacterial activity from a livestock feed prebiotic supplement. a novel strain, termed paenibacillus polymyxa jb05-01-1, was isolated using traditional microbiological methods and identified on the basis of its phenotypic and biochemical properties as well as its 16s rrna gene sequence. this strain was able to inhibit growth of gram-negative bacteria including escherichia coli rr1, pseudomonas fluores ... | 2010 | 21132276 |
| isolation and characterisation of bacterial colonies from seeds and in vitro cultures of fraxinus spp. from italian sites. | culturable bacteria were isolated from seeds, embryos and contaminated in vitro cultures of ash (fraxinus excelsior l., f. ornus l. and f. angustifolia l.) and were identified using morphological and molecular analyses. fourteen morphologically distinct isolates were recovered from seeds of fraxinus spp. 16s rdna sequencing categorised these isolates into ten separate genera. three strains isolated from contaminated in vitro cultures, pantoea agglomerans, staphylococcus succinus and aerococcus v ... | 2011 | 21143738 |
| erwinia oleae sp. nov., isolated from olive knots caused by pseudomonas savastanoi pv. savastanoi. | three endophytic bacterial isolates were obtained in italy from olive knots caused by pseudomonas savastanoi pv. savastanoi. phenotypic tests in combination with 16s rrna gene sequence analysis indicated a phylogenetic position of these isolates in the genus erwinia or pantoea, and revealed two other strains with highly similar 16s rrna gene sequences (> 99 %), cect 5262 and cect 5264, obtained in spain from olive knots. rep-pcr dna fingerprinting of the five strains from olive knots with box, e ... | 2010 | 21186287 |
| bacterial and fungal communities of wilted italian ryegrass silage inoculated with and without lactobacillus rhamnosus or lactobacillus buchneri. | to understand the effects of lactic acid bacteria (lab) inoculation on fermentation products, aerobic stability and microbial communities of silage. | 2011 | 21204884 |
| soil type affects plant colonization, activity and catabolic gene expression of inoculated bacterial strains during phytoremediation of diesel. | the combined use of plants and associated microorganisms has great potential for cleaning up soils contaminated with petroleum hydrocarbons. apart from environmental conditions the physicochemical properties of the soil are the main factors influencing the survival and activity of an inoculated strain as well as the growth of plants. this study examined the effect of different soil types (sandy, loamy sand and loam) on the survival, gene abundance and catabolic gene expression of two inoculated ... | 2010 | 21216097 |
| multiple treatment meta-analysis of products evaluated for control of fire blight in the eastern united states. | the aim of this analysis was to estimate the effect sizes and consistency of products evaluated for fire blight control in the eastern united states over the last decade. because only 3% of the 69 studies published from 2000 to 2008 explicitly presented a measure of within-study variability, a method for estimating the least significant difference (lsd) and, hence the sampling variance, for studies with at least two significant mean separations in the presented mean multiple comparisons was deve ... | 2011 | 21244224 |
| enterobacterial microflora in infancy - a case study with enhanced enrichment. | to validate pmeu (portable microbe enrichment unit) method for monitoring the composition and development of infantile intestinal enterobacterial microflora. | 2011 | 21249478 |
| characterization of the rcsc sensor kinase from erwinia amylovora and other enterobacteria. | rcsc is a hybrid sensor kinase which contains a sensor domain, a histidine kinase domain, and a receiver domain. we have previously demonstrated that, although the erwinia amylovora rcsc mutant produces more amylovoran than the wild-type (wt) strain in vitro, the mutant remains nonpathogenic on both immature pear fruit and apple plants. in this study, we have comparatively characterized the erwinia rcsc and its homologs from various enterobacteria. results demonstrate that expression of the erwi ... | 2011 | 21261468 |
| differences in bacterial diversity of host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory. | host-associated differentiation (had) is the presence of genetically divergent, host-associated populations. it has been suggested that microbial symbionts of insect herbivores may play a role in had by allowing their insect hosts to use different plant species. the objective of this study was to document if host-associated populations of phylloxera notabilis pergande (hemiptera: phylloxeridae) in pecan and water hickory corresponded with differences in the composition of their associated bacter ... | 2011 | 21261774 |
| [isolation, identification and characterization of acc deaminase-containing endophytic bacteria from halophyte suaeda salsa]. | we isolated and characterized 1-aminocyclopropane-1-carboxylate (acc) deaminase-containing endophytic bacteria from halophyte suaeda salsa to understand the interactions between endophytes and halophyte. | 2010 | 21268896 |
| phosphate-solubilization mechanism and in vitro plant growth promotion activity mediated by pantoea eucalypti isolated from lotus tenuis rhizosphere in the salado river basin (argentina). | to isolate and characterize phosphate-solubilizing strains from a constrained environment such as the salado river basin and to assess their phosphate-solubilizing mechanisms, to further selection of the most promising strains to inoculate and improve the implantation and persistence of lotus tenuis in the most important area devoted to meat-cow production in argentina. | 2011 | 21299771 |
| identification of pantoea ananatis gene encoding membrane pyrroloquinoline quinone (pqq)-dependent glucose dehydrogenase and pqqabcdef operon essential for pqq biosynthesis. | pantoea ananatis accumulates gluconate during aerobic growth in the presence of glucose. computer analysis of the p. ananatis sc17(0) sequenced genome revealed an orf encoding a homologue (named gcd) of the mgdh (ec 1.1.99.17) apoenzyme from escherichia coli and a putative pyrroloquinoline quinone (pqq) biosynthetic operon homologous to pqqabcdef from klebsiella pneumoniae. construction of ?gcd and ?pqq mutants of p. ananatis confirmed the proposed functions of these genetic elements. the p. ana ... | 2011 | 21306430 |
| characterization of acc deaminase-producing endophytic bacteria isolated from copper-tolerant plants and their potential in promoting the growth and copper accumulation of brassica napus. | one hundred cu-resistant-endophytic bacteria were isolated from cu-tolerant plants grown on cu mine wasteland, of which, eight cu-resistant and 1-aminocyclopropane-1-carboxylate (acc) deaminase-producing endophytic bacteria were obtained based on the acc deaminase activity of the bacteria and characterized with respect to metal resistance, production of acc deaminase, indole-3-acetic acid (iaa) as well as siderophores and mineral phosphate solubilization. ralstonia sp. j1-22-2, pantoea agglomera ... | 2011 | 21315404 |
| bacterial species associated with sound and botrytis-infected grapes from a greek vineyard. | grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ... | 2011 | 21315469 |
| the effect of handwashing with water or soap on bacterial contamination of hands. | handwashing is thought to be effective for the prevention of transmission of diarrhoea pathogens. however it is not conclusive that handwashing with soap is more effective at reducing contamination with bacteria associated with diarrhoea than using water only. in this study 20 volunteers contaminated their hands deliberately by touching door handles and railings in public spaces. they were then allocated at random to (1) handwashing with water, (2) handwashing with non-antibacterial soap and (3) ... | 2011 | 21318017 |
| optimization of medium for indole-3-acetic acid production using pantoea agglomerans strain pvm. | to optimize the medium components for the production of indole-3-acetic acid (iaa) by isolated bacterium pantoea agglomerans strain pvm. | 2011 | 21332896 |
| dynamics and species diversity of communities of lactic acid bacteria and acetic acid bacteria during spontaneous cocoa bean fermentation in vessels. | to speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was developed successfully. therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20 kg scale were tried out in triplicate. the community dynamics and kinetics of these fermentations were studied through a multiphasic approach. microbiological analysis revealed a limited bacterial s ... | 2010 | 21356451 |
| cellulose-degrading bacteria associated with the invasive woodwasp sirex noctilio. | sirex noctilio is an invasive wood-feeding wasp that threatens the world's commercial and natural pine forests. successful tree colonization by this insect is contingent on the decline of host defenses and the ability to utilize the woody substrate as a source of energy. we explored its potential association with bacterial symbionts that may assist in nutrient acquisition via plant biomass deconstruction using growth assays, culture-dependent and -independent analysis of bacterial frequency of a ... | 2011 | 21368904 |
| characterization of nuclear localization signals in the type iii effectors hsvg and hsvb of the gall-forming bacterium pantoea agglomerans. | hsvg and hsvb, two paralogous type iii effectors of the gall-forming bacteria pantoea agglomerans pv. gypsophilae and p. agglomerans pv. betae, determine host specificity on gypsophila and beet, respectively. they were previously shown to be dna-binding proteins imported into host and non-host nuclei and might act as transcriptional activators. sequence analysis of these effectors did not detect canonical nuclear localization signals (nlss), but two basic amino acid clusters designated putative ... | 2011 | 21372093 |
| spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. | the spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. the proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (dgge) and dna sequencing in order to relate them to the performance and stability of the bioelectrochemical system. the reproducibility o ... | 2011 | 21400098 |
| bacteriophages limelight and limezero of pantoea agglomerans, belonging to the "phikmv-like viruses". | pantoea agglomerans is a common soil bacterium used in the biocontrol of fungi and bacteria but is also an opportunistic human pathogen. it has been described extensively in this context, but knowledge of bacteriophages infecting this species is limited. bacteriophages limezero and limelight of p. agglomerans are lytic phages, isolated from soil samples, belonging to the podoviridae and are the first pantoea phages of this family to be described. the double-stranded dna (dsdna) genomes (43,032 b ... | 2011 | 21421778 |
| improved resistance against botrytis cinerea by grapevine-associated bacteria that induce a prime oxidative burst and phytoalexin production. | bacteria such as pantoea agglomerans (pa-af2), bacillus subtilis (bs-271), acinetobacter lwoffii (al-113), and pseudomonas fluorescens (pf-ct2), originating from the vineyard, can induce defense responses and enhance resistance of grapevine against the fungal pathogen botrytis cinerea. the perception of these bacteria by plant cells or tissues in relation to their activities remains unknown. in this study, we examined the relationships between the activity of each bacterium to induce or prime so ... | 2011 | 21425931 |
| plant growth promotion traits of phosphobacteria isolated from puna, argentina. | the ability of soil microorganisms to solubilize phosphate is an important trait of plant growth-promoting bacteria leading to increased yields and smaller use of fertilizers. this study presents the isolation and characterization of phosphobacteria from puna, northwestern argentina and the ability to produce phosphate solubilization, alkaline phosphatase, siderophores, and indole acetic acid. the p-solubilizing activity was coincidental with a decrease in ph values of the tricalcium phosphate m ... | 2011 | 21442320 |
| unusual causes of peritonitis in a peritoneal dialysis patient: alcaligenes faecalis and pantoea agglomerans. | an 87 -year-old female who was undergoing peritoneal dialysis presented with peritonitis caused by alcaligenes faecalis and pantoea agglomerans in consecutive years. with the following report we discuss the importance of these unusual microorganisms in peritoneal dialysis patients. | 2011 | 21477370 |
| changes in bacterial flora of japanese cabbage during growth and potential source of flora. | bacterial flora of cabbage were identified and enumerated during various stages of growth, and the potential sources of contamination in the field were determined. bacterial counts increased from below the level of detection (2.4 log cfu/g) on seeds to 2.5 to 5.7 log cfu/g on seedlings. after transplanting, the counts of mesophilic aerobic bacteria on leaves decreased and then increased to 5.7 log cfu/g on outer leaves, 5.0 log cfu/g on middle leaves, and 3.0 log cfu/g on inner leaves at the har ... | 2011 | 21477482 |
| pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. | a. shubov, p. jagannathan, p. v. chin-hong. pantoea agglomerans pneumonia in a heart-lung transplant recipient: case report and a review of an emerging pathogen in immunocompromised hosts. transpl infect dis 2011. all rights reserved abstract: pantoea agglomerans is a gram-negative rod that is frequently found on the exterior of many plants, fruits, vegetables, and in soil, and it is used as a biopesticide in the agriculture industry. recent reports have implicated p. agglomerans in systemic inf ... | 2011 | 21504526 |
| bacterial community diversity and variation in spray water sources and the tomato fruit surface. | abstract: | 2011 | 21510867 |
| oral administration of immunopotentiator from pantoea agglomerans 1 (ip-pa1) improves the survival of b16 melanoma-inoculated model mice. | to investigate the usefulness of the immunopotentiator from pantoea agglomerans 1 (ip-pa1) as a supportive drug in melanoma therapy, we analyzed the immunological effects of ip-pa1 on melanoma-inoculated model mice. oral administration of ip-pa1 increased the serum levels of tumor necrosis factor (tnf)-α at 2 h after the administration and interferon (ifn)-γ and il-12 at 12 h after the administration in naïve balb/ccrslc mice as evaluated by elisa. ip-pa1 did not affect the proliferation of mela ... | 2011 | 21512265 |
| a head-to-head comparison of eneamide and epoxyamide inhibitors of glucosamine-6-phosphate synthase from the dapdiamide biosynthetic pathway. | the dapdiamides make up a family of antibiotics that have been presumed to be cleaved in the target cell to enzyme-inhibitory n-acyl-2,3-diaminopropionate (dap) warheads containing two alternative electrophilic moieties. our prior biosynthetic studies revealed that an eneamide warhead is made first and converted to an epoxyamide via a three-enzyme branch pathway. here we provide a rationale for this logic. we report that the r,r-epoxyamide warhead is a more efficient covalent inactivator of gluc ... | 2011 | 21520904 |
| engineering of a plasmid-free escherichia coli strain for improved in vivo biosynthesis of astaxanthin. | abstract: background: the xanthophyll astaxanthin is a high-value compound with applications in the nutraceutical, cosmetic, food, and animal feed industries. besides chemical synthesis and extraction from naturally producing organisms like haematococcus pluvialis, heterologous biosynthesis in non-carotenogenic microorganisms like escherichia coli, is a promising alternative for sustainable production of natural astaxanthin. recent achievements in the metabolic engineering of e. coli strains hav ... | 2011 | 21521516 |
| classification of plant associated bacteria using rif, a computationally derived dna marker. | a dna marker that distinguishes plant associated bacteria at the species level and below was derived by comparing six sequenced genomes of xanthomonas, a genus that contains many important phytopathogens. this dna marker comprises a portion of the dnaa replication initiation factor (rif). unlike the rrna genes, dnaa is a single copy gene in the vast majority of sequenced bacterial genomes, and amplification of rif requires genus-specific primers. in silico analysis revealed that rif has equal or ... | 2011 | 21533033 |
| kinetics of bacterial potentiometric titrations: the effect of equilibration time on buffering capacity of pantoea agglomerans suspensions. | several recent studies have made use of continuous acid-base titration data to describe the surface chemistry of bacterial cells as a basis for accurately modelling metal adsorption to bacteria and other biomaterials of potential industrial importance. these studies do not share a common protocol; rather they titrate in different ph ranges and they use different stability criteria to define equilibration time during titration. in the present study we investigate the kinetics of bacterial titrati ... | 2011 | 21543082 |
| endophytic bacteria in the rice plant. | endophytic bacteria are defined as bacteria detected inside surface-sterilized plants or extracted from inside plants and having no visibly harmful effects on the plants. various kinds of endophytic bacteria, such as pantoea, methylobacterium, azospirillum, herbaspirillum, burkholderia and rhizobium etc., have been found inside rice plants. this minireview summarizes and discusses recent studies of endophytic bacteria residing in rice plants, focusing on flora, origin, movement, and interaction ... | 2008 | 21558696 |
| a novel low-temperature-active β-glucosidase from symbiotic serratia sp. tn49 reveals four essential positions for substrate accommodation. | a 2,373-bp full-length gene (bgla49) encoding a 790-residue polypeptide (bgla49) with a calculated mass of 87.8 kda was cloned from serratia sp. tn49, a symbiotic bacterium isolated from the gut of longhorned beetle (batocera horsfieldi) larvae. the deduced amino acid sequence of bgla49 showed the highest identities of 80.1% with a conceptually translated protein from pantoea sp. at-9b (eew02556), 38.3% with the identified glycoside hydrolase (gh) family 3 β-glucosidase from clostridium stercora ... | 2011 | 21559826 |
| stereochemistry and mechanism of a microbial phenylalanine aminomutase. | the stereochemistry of a phenylalanine aminomutase (pam) on the andrimid biosynthetic pathway in pantoea agglomerans (pa) is reported. papam is a member of the 4-methylidene-1h-imidazol-5(4h)-one (mio)-dependent family of catalysts and isomerizes (2s)-α-phenylalanine to (3s)-β-phenylalanine, which is the enantiomer of the product made by the mechanistically similar aminomutase tcpam from taxus plants. the nh(2) and pro-(3s) hydrogen groups at c(α) and c(β), respectively, of the substrate are rem ... | 2011 | 21561099 |
| rice seeds as sources of endophytic bacteria. | endophytic bacteria are considered to originate from the external environment. to examine the hypothesis that rice (oryza sativa, cultivar kinuhikari) seeds are a source of endophytic bacteria, we isolated endophytic bacteria from the shoots, remains of the seeds, and roots of rice seedlings that were aseptically cultivated in vitro from surface-disinfected seeds. of the various bacterial strains isolated, the closest relatives, identified by 16s rrna gene sequencing, were: bacillus firmus, b. f ... | 2009 | 21566368 |
| comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in ivory coast and brazil. | to compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one ivorian farm) and box (two brazilian farms) fermentations were carried out. all fermentations were studied through a multiphasic approach. in general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48°c. the main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mann ... | 2011 | 21569940 |
| population dynamics of bacteria associated with different strains of the pine wood nematode bursaphelenchus xylophilus after inoculation in maritime pine (pinus pinaster). | for a long time it was thought that bursaphelenchus xylophilus was the only agent of the pine wilt disease. recently, it was discovered that there are bacteria associated with the nematodes that contribute to the pathogenesis of this disease, mainly through the release of toxins that promote the death of the pines. among the species most commonly found, are bacteria belonging to the bacillus, pantoea, pseudomonas and xanthomonas genera. the main objective of this work was to study the effect of ... | 2011 | 21570967 |
| exopolysaccharide production is influenced by sugars, n-acylhomoserine lactone, and transcriptional regulators rcsa and rcsb, but does not affect pathogenicity in the plant pathogen pantoea ananatis. | pantoea ananatis sk-1 produced eps by ahl-mediated quorum sensing on an lb agar plate containing glucose, fructose, and sucrose. rcsa and rcsb mutants did not produce eps with or without ahls and with or without sugars, but they induced necrotic symptoms in onion leaves. these results indicate that eps production does not relate to the pathogenicity of sk-1. | 2011 | 21597171 |
| secretion of anti-plasmodium effector proteins from a natural isolate pantoea agglomerans using pelb and hlya secretion signals. | the insect-vectored disease malaria is a major world health problem. new control strategies are needed to supplement the current use of insecticides and medications. a genetic approach can be used to inhibit development of the malaria parasite (plasmodium sp.) in the mosquito host. we hypothesized that pantoea agglomerans, a bacterial symbiont of anopheles mosquitoes, could be engineered to express and secrete anti-plasmodium effector proteins, a strategy termed paratransgenesis. to this end, pl ... | 2011 | 21602368 |
| directed evolution of the nonribosomal peptide synthetase admk generates new andrimid derivatives in vivo. | many lead compounds in the search for new drugs derive from peptides and polyketides whose similar biosynthetic enzymes have been difficult to engineer for production of new derivatives. problems with generating multiple analogs in a single experiment along with lack of high-throughput methods for structure-based screening have slowed progress in this area. here, we use directed evolution and a multiplexed assay to screen a library of >14,000 members to generate three derivatives of the antibact ... | 2011 | 21609841 |
| characterization of a small cryptic plasmid from endophytic pantoea agglomerans and its use in the construction of an expression vector. | a circular cryptic plasmid named ppaga (2,734 bp) was isolated from pantoea agglomerans strain ege6 (an endophytic bacterial isolate from eucalyptus). sequence analysis revealed that the plasmid has a g+c content of 51% and contains four potential orfs, 238(a), 250(b), 131(c), and 129(d) amino acids in length without homology to known proteins. the shuttle vector plgm1 was constructed by combining the ppaga plasmid with pgfpmut3.0 (which harbors a gene encoding green fluorescent protein, gfp), a ... | 2011 | 21637551 |
| rhizosphere competent pantoea agglomerans enhances maize (zea mays) and chickpea (cicer arietinum l.) growth, without altering the rhizosphere functional diversity. | plant growth promoting pantoea agglomerans nbrisrm (nbrisrm) was able to produce 60.4 μg/ml indole acetic acid and solubilize 77.5 μg/ml tri-calcium phosphate under in vitro conditions. addition of 2% nacl (w/v) in the media induced the iaa production and phosphate solubilization by 11% and 7%, respectively. for evaluating the plant growth promotory effect of nbrisrm inoculation a micro plot trial was conducted using maize and chickpea as host plants. the results revealed significant increase in ... | 2011 | 21638110 |
| diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis. | microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ... | 2011 | 21660545 |
| conservation and diversity of seed associated endophytes in zea across boundaries of evolution, ethnography and ecology. | endophytes are non-pathogenic microbes living inside plants. we asked whether endophytic species were conserved in the agriculturally important plant genus zea as it became domesticated from its wild ancestors (teosinte) to modern maize (corn) and moved from mexico to canada. kernels from populations of four different teosintes and 10 different maize varieties were screened for endophytic bacteria by culturing, cloning and dna fingerprinting using terminal restriction fragment length polymorphis ... | 2011 | 21673982 |