| prevalence of chronic hyperplasic candidiasis. its association to risk factors in an oral medicine service in rosario, argentina. | chronic hyperplasic candidiasis (chc) is one of clinical forms of infection caused by fungi from genus candida, basically candida albicans, and less frequently by other species of the genus, such as candida tropicalis, candida guilliermondii, candida glabrata, candida parapsilosis, candida dubliniensis y candida krusei. | 2012 | 23177812 |
| [yeast colonization of urinary catheters and the significance of biofilm formation]. | urinary catheters are colonized by a wide range of microorganisms, including numerous yeasts. the catheters are usually colonized by more microbial species forming a community - multispecies biofilm. catheter colonization usually does not affect the patient's clinical status in any significant way. on the other hand, the biofilm can become a source of endogenous infection and its presence can affect functionality of the catheter and formation of urinary stones. material a | 2012 | 23172023 |
| differentiation of candida dubliniensis from candida albicans by means of maldi-tof mass spectrometry. | species specific differentiation of the two closely related yeasts, candida albicans and c. dubliniensis, is difficult in routine diagnosis. | 2012 | 23163108 |
| effect of different pre-irradiation times on curcumin-mediated photodynamic therapy against planktonic cultures and biofilms of candida spp. | the aim of this study was to evaluate the effects of pre-irradiation time (pit) on curcumin (cur)-mediated photodynamic therapy (pdt) against planktonic and biofilm cultures of reference strains of candida albicans, candida glabrata and candida dubliniensis. | 2013 | 23153629 |
| rapid and accurate identification of isolates of candida species by melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca). | rapid identification of clinically important yeasts can facilitate the initiation of anti-fungal therapy, since susceptibility is largely species-dependent. we evaluated melting peak and melting curve analysis of the internally transcribed spacer region 2 fragment (its2-mca) as an identification tool for distinguishing between 16 candida spp., i.e. candida albicans, candida bracarensis, candida dubliniensis, candida famata, candida glabrata, candida guilliermondii, candida inconspicua, candida k ... | 2013 | 23142490 |
| use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva. | a pcr-denaturing gradient gel electrophoresis (dgge) method was established for the simultaneous presumptive identification of multiple yeast species commonly present in the oral cavity. published primer sets targeting different regions of the saccharomyces cerevisiae 26-28s rrna gene (denoted primer sets n and u) and the 18s rrna gene (primer set e) were evaluated with ten candida and four non-candida yeast species, and twenty candida albicans isolates. optimized pcr-dgge conditions using prime ... | 2013 | 23065546 |
| performance comparison of phenotypic and molecular methods for detection and differentiation of candida albicans and candida dubliniensis. | candida albicans is the most pathogenic candida species but shares many phenotypic features with candida dubliniensis and may, therefore, be misidentified in clinical microbiology laboratories. candidemia cases due to c. dubliniensis are increasingly being reported in recent years. accurate identification is warranted since mortality rates are highest for c. albicans infections, however, c. dubliniensis has the propensity to develop resistance against azoles more easily. we developed a duplex pc ... | 2012 | 23009343 |
| oral candida colonization in hiv-positive women: associated factors and changes following antiretroviral therapy. | there is a paucity of information about the factors associated with oral colonization with candida species and the changes associated with antiretroviral (arv) therapy. this study investigated the role of arv therapy and other factors in a study population. relevant clinical and laboratory information was obtained and oral rinse specimens were tested for yeast identification. the findings were compared with previous data from the same clinic before arv therapy was available. of 197 patients, 117 ... | 2013 | 23002070 |
| predisposing factors for oropharyngeal colonization of yeasts in human immunodeficiency virus-infected patients: a prospective cross-sectional study. | oropharyngeal candidiasis continues to be a major opportunistic infection in human immunodeficiency virus (hiv)-infected patients. the objectives of this study were to investigate the prevalence, associated factors, and microbiologic features for oropharyngeal yeast colonization in hiv-infected patients. | 2013 | 22921200 |
| transcript profiling reveals rewiring of iron assimilation gene expression in candida albicans and c. dubliniensis. | hyphal growth is repressed in candida albicans and candida dubliniensis by the transcription factor nrg1. transcript profiling of a c. dubliniensis nrg1 mutant identified a common group of 28 nrg1-repressed genes in both species, including the hypha-specific genes hwp1, ece1 and the regulator of cell elongation ume6. unexpectedly, c. dubliniensis nrg1 was required for wild-type levels of expression of 10 genes required for iron uptake including seven ferric reductases, sit1, ftr1 and rbt5. howev ... | 2012 | 22888912 |
| differential interaction of the two related fungal species candida albicans and candida dubliniensis with human neutrophils. | candida albicans, the most common facultative human pathogenic fungus is of major medical importance, whereas the closely related species candida dubliniensis is less virulent and rarely causes life-threatening, systemic infections. little is known, however, about the reasons for this difference in pathogenicity, and especially on the interactions of c. dubliniensis with the human immune system. because innate immunity and, in particular, neutrophil granulocytes play a major role in host antifun ... | 2012 | 22851712 |
| stearidonic acid acts in synergism with amphotericin b in inhibiting candida albicans and candida dubliniensis biofilms in vitro. | | 2012 | 22817915 |
| a simple and reliable pcr-restriction fragment length polymorphism assay to identify candida albicans and its closely related candida dubliniensis. | candida dubliniensis is an emerging pathogen capable of causing superficial as well as systemic infections. due to its close similarity to c. albcians, conventional methods based on phenotypic traits are not always reliable in identification of c. dubliniensis. in this study, we developed a pcr-restriction fragment length polymorphism (rflp) assay to identify and discriminate between the two closely related species. the d1/d2 region of 28s rdna was amplified by pcr and enzymatically digested by ... | 2012 | 24031901 |
| [virulence of candida dubliniensis in comparison with candida albicans using an experimental model of mouse oral candiddiasis]. | certain species of candida are known as opportunistic fungal pathogens and candida albicans has especially been isolated oral candidiasis patients at high frequency as a result of its strong pathogenicity. recently c. dubliniensis is isolated mainly from immunocompromised patients, but is also detected from healthy persons. c. dubliniensis has similar cell morphology and molecular biological properties to c. albicans. thus, in order to clarify the pathogenicity of c. dubliniensis, the activities ... | 2012 | 22728597 |
| oral candidosis in lichen planus: the diagnostic approach is of major therapeutic importance. | candida albicans is the most common fungal pathogen in humans, but other candida species cause candidosis. candida species display significant differences in their susceptibility to antimycotic drugs. patients with symptomatic or erythematous oral lichen planus (olp) commonly have candida infection requiring correct identification of candida species in order to initiate adequate antimycotic therapy. therefore, conventional cytosmear and culture tests were compared with genetic diagnostics on ora ... | 2013 | 22699660 |
| molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults. | to characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status. | 2012 | 22642872 |
| sequence and analysis of the genome of the pathogenic yeast candida orthopsilosis. | candida orthopsilosis is closely related to the fungal pathogen candida parapsilosis. however, whereas c. parapsilosis is a major cause of disease in immunosuppressed individuals and in premature neonates, c. orthopsilosis is more rarely associated with infection. we sequenced the c. orthopsilosis genome to facilitate the identification of genes associated with virulence. here, we report the de novo assembly and annotation of the genome of a type 2 isolate of c. orthopsilosis. the sequence was o ... | 2012 | 22563396 |
| the tlo proteins are stoichiometric components of candida albicans mediator anchored via the med3 subunit. | the amplification of the tlo (for telomere-associated) genes in candida albicans, compared to its less pathogenic, close relative candida dubliniensis, suggests a role in virulence. little, however, is known about the function of the tlo proteins. we have purified the mediator coactivator complex from c. albicans (camediator) and found that tlo proteins are a stoichiometric component of camediator. many members of the tlo family are expressed, and each is a unique member of camediator. protein e ... | 2012 | 22562472 |
| potent antifungal activity of pure compounds from traditional chinese medicine extracts against six oral candida species and the synergy with fluconazole against azole-resistant candida albicans. | this study was designed to evaluate the in vitro antifungal activities of four traditional chinese medicine (tcm) extracts. the inhibitory effects of pseudolaric acid b, gentiopicrin, rhein, and alion were assessed using standard disk diffusion and broth microdilution assays. they were tested against six oral candida species, candida albicans, candida glabrata, candida tropicalis, candida krusei, candida dubliniensis, and candida guilliermondii, including clinical isolates from hiv-negative, hiv ... | 2012 | 22454653 |
| rapid differentiation of candida dubliniensis from candida albicans by early d-xylose assimilation. | to determine if d-xylose (xyl) and/or α-methyl-d-glucoside (mdg) assimilation can be used reliably as a rapid test to differentiate candida dubliniensis from candida albicans at an earlier time point such as 2 h after inoculation. | 2012 | 22398877 |
| antimicrobial properties and cytotoxicity of an antimicrobial monomer for application in prosthodontics. | this study aimed to investigate the antimicrobial properties and cytotoxicity of the monomer methacryloyloxyundecylpyridinium bromide (mupb), an antiseptic agent capable of copolymerizing with denture base acrylic resins. | 2012 | 22339776 |
| oral candida carriage, quantification, and species characterization in oral submucous fibrosis patients and healthy individuals. | to determine the prevalence of oral candida carriage, candidal quantification, and various subtypes of candida species in oral submucous fibrosis patients and healthy individuals. | 2011 | 25426899 |
| [candida dubliniensis: need for correct diagnosis]. | a study of 115 strains, which had been isolated from vaginal smears and identified at the beginning as candida albicans, was conducted to find out if there was candida dubliniensis in cuba. the growth of those isolates that due to their morphological characteristics were identifified as either c. albicans or c. dubliniensis was checked up at 42 degrees in sabouraud agar. c. albicans was the predominant species (88.69%). for the first time in cuba, 13 c. dubliniensis strains were presumptively id ... | 2013 | 23424796 |
| impact of short-term exposure of antifungal agents on hemolysin activity of oral candida dubliniensis isolates from kuwait and sri lanka. | ability to produce hemolysin by candida species is an important determinant of its pathogenicity. candida dubliniensis is implicated in the causation of oral candidosis, which can be treated with polyene, echinocandin, and azole groups of antifungal agents as well as chlorhexidine. after oral application, however, the concentrations of these agents tend to decrease quickly to subtherapeutic levels due to the peculiarity of the oral environment. in this study, we have evaluated the effect of shor ... | 2016 | 26059506 |
| candida dubliniensis pneumonia: a case report and review of literature. | candida dubliniensis is an uncommon species of candida which has been implicated in fungal pneumonia only very rarely. we present the case of a 75-year-old man with laryngeal cancer undergoing chemotherapy on broad-spectrum antibiotics and tuberculosis therapy with blood and endotracheal cultures positive for c. dubliniensis. subsequent autopsy was performed with postmortem lung cultures positive for c. dubliniensis and lung histopathology demonstrating an invasive fungal infection. molecular an ... | 2016 | 27342836 |
| experimental conical-head abutment screws on the microbial leakage through the implant-abutment interface: an in vitro analysis using target-specific dna probes. | the aim of this in vitro study was to identify and quantify up to 38 microbial species from human saliva penetrating through the implant-abutment interface in two different implant connections, external hexagon and tri-channel internal connection, both with conventional flat-head or experimental conical-head abutment screws. | 2016 | 27198721 |
| meningitis caused by candida dubliniensis in a patient with cirrhosis: a case report and review of the literature. | candida species, including candida dubliniensis, are a rare cause of meningitis. herein, we report the second case of c. dubliniensis meningitis in a 49-year-old man with a history of hepatitis c virus-related cirrhosis, substance use disorder, and recent exposure to intravenous antibiotic therapy, presenting with confusion, abnormal gait, and urinary incontinence. magnetic resonance imaging (mri) of the brain showed marked hydrocephalus and leptomeningeal enhancement. initial cerebrospinal flui ... | 2016 | 27038312 |
| survey and analysis of simple sequence repeats (ssrs) in three genomes of candida species. | simple sequence repeats (ssrs) or microsatellites, which composed of tandem repeated short units of 1-6 bp, have been paying attention continuously. here, the distribution, composition and polymorphism of microsatellites and compound microsatellites were analyzed in three available genomes of candida species (candida dubliniensis, candida glabrata and candida orthopsilosis). the results show that there were 118,047, 66,259 and 61,119 microsatellites in genomes of c. dubliniensis, c. glabrata and ... | 2016 | 26883055 |
| oral administration of the broad-spectrum antibiofilm compound toremifene inhibits candida albicans and staphylococcus aureus biofilm formation in vivo. | we here report on the in vitro activity of toremifene to inhibit biofilm formation of different fungal and bacterial pathogens, including candida albicans, candida glabrata, candida dubliniensis, candida krusei, pseudomonas aeruginosa, staphylococcus aureus, and staphylococcus epidermidis. we validated the in vivo efficacy of orally administered toremifene against c. albicans and s. aureus biofilm formation in a rat subcutaneous catheter model. combined, our results demonstrate the potential of ... | 2014 | 25288093 |
| [candida dubliniensis in clinical specimens and possibilities for identification]. | the species candida dubliniensis shares a wide range of phenotypic characteristics with candida albicans, the most common yeast species isolated from clinical specimens. this is a considerable complication for the detection and identification of candida dubliniensis from clinical specimens. the lack of data on the incidence of c. dubliniensis in the czech republic was the motivation behind the efforts to detect this pathogen in specimens analyzed at the institute for microbiology, faculty of med ... | 2014 | 25025678 |
| prevalence of candida albicans-closely related yeasts, candida africana and candida dubliniensis, in vulvovaginal candidiasis. | isolates of candida africana and c. dubliniensis were recovered from patients with vulvovaginal candidiasis (vvc). the isolates were initially identified as c. albicans through use of the api candida system. we retrospectively reexamined 1014 vaginal isolates presumptively determined to be c. albicans at the department of obstetrics and gynecology of peking university shenzhen hospital from 1 january 2003 through 31 december 2012. our objective was to determine, via detection of the hwp1 gene, i ... | 2014 | 25023482 |
| yeast oropharyngeal colonization in human immunodeficiency virus-infected patients in central taiwan. | a prospective, cross-sectional study was conducted at a medical center in central taiwan to understand the prevalence, associated factors, and microbiologic features for oropharyngeal yeast colonization in human immunodeficiency virus-infected outpatients. oral yeast colonization was detected in 127 (45 %) patients, including 21 (16.5 %) colonized by more than one species. of the 154 isolates, candida albicans was the most common species (114, 74 %), followed by candida dubliniensis (10, 6.5 %), ... | 2014 | 24804977 |
| multi-drug resistant oral candida species isolated from hiv-positive patients in south africa and cameroon. | candida species are a common cause of infection in immune-compromised hiv-positive individuals, who are usually treated with the antifungal drug, fluconazole, in public hospitals in africa. however, information about the prevalence of drug resistance to fluconazole and other antifungal agents on candida species is very limited. this study examined 128 candida isolates from south africa and 126 cameroonian candida isolates for determination of species prevalence and antifungal drug susceptibility ... | 2014 | 24726686 |
| species distribution and antifungal susceptibility profile of candida isolates from bloodstream infections in lima, peru. | yeast identification and in vitro susceptibility testing provide helpful information for appropriate administration of antifungal treatments; however, few reports from the latin american region have been published. the aim of this study was to identify the species present in isolates from bloodstream infections diagnosed in nine hospitals in lima, peru and to determine their in vitro susceptibility to four antifungal drugs. we tested and identified 153 isolates collected between october 2009 and ... | 2014 | 24667770 |
| association of clinical and demographic factors in invasive candidiasis caused by fluconazole-resistant candida species: a study in 15 hospitals, medellín, colombia 2010-2011. | candida is the most important agent of fungal infections. several risk factors have been described associated with invasive infection by fluconazole-resistant candida spp. a prospective cross-sectional study with case-control analysis was conducted. case group patients with fluconazole-resistant candida isolate were included; control group were patients with fluconazole-susceptible candida spp. a multivariate logistic regression model was performed. three hundred isolates of candida spp. were an ... | 2014 | 24666706 |
| the development and validation of a rapid genetic method for species identification and genotyping of medically important fungal pathogens using high-resolution melting curve analysis. | accurate, rapid and economical fungal species identification has been a major aim in mycology. in this study, our goal was to examine the feasibility of a high-resolution melting curve analysis (hrma) of internal transcribed regions its1 and its2 in ribosomal dna (rdna) for a rapid, simple and inexpensive differentiation of eight clinically relevant candida species (candida albicans, candida glabrata, candida parapsilosis, candida krusei, candida tropicalis, candida guilliermondii, candida dubli ... | 2014 | 24628973 |
| comparative adherence of candida albicans and candida dubliniensis to human buccal epithelial cells and extracellular matrix proteins. | candida albicans and candida dubliniensis are very closely related pathogenic yeast species. despite their close relationship, c. albicans is a far more successful colonizer and pathogen of humans. the purpose of this study was to determine if the disparity in the virulence of the two species is attributed to differences in their ability to adhere to human buccal epithelial cells (becs) and/or extracellular matrix proteins. when grown overnight at 30°c in yeast extract peptone dextrose, genotype ... | 2014 | 24625677 |
| oral candida carriage and immune status in thai human immunodeficiency virus-infected individuals. | oral candidiasis is a common opportunistic infection among human immunodeficiency virus (hiv)-infected individuals, with growing concerns about the emergence of non-albicans species with resistance to antifungal agents. this cross-sectional study determined the prevalence of oral candida species in thai hiv-infected adults and factors affecting their colonization. candida species were identified from oral rinse samples of 60 hiv-infected participants of the mtct-plus initiative and 49 healthy co ... | 2014 | 24591706 |
| the effect of psychoactive substances (drugs) on the presence and frequency of oral candida species and candida dubliniensis. | the goal of this study was to determine the effect of psychoactive substances (drugs) on the presence and frequency of oral candida species and candida dubliniensis. | 2013 | 24511261 |
| fluconazole and amphotericin-b resistance are associated with increased catalase and superoxide dismutase activity in candida albicans and candida dubliniensis. | candida dubliniensis, a new species of candida that has been recovered from several sites in healthy people, has been associated with recurrent episodes of oral candidiasis in aids and hiv-positive patients. this species is closely related to c. albicans. the enzymatic activity of c. dubliniensis in response to oxidative stress is of interest for the development of drugs to combat c. dubliniensis. | 2014 | 24474018 |
| calcineurin controls hyphal growth, virulence, and drug tolerance of candida tropicalis. | candida tropicalis, a species closely related to candida albicans, is an emerging fungal pathogen associated with high mortality rates of 40 to 70%. like c. albicans and candida dubliniensis, c. tropicalis is able to form germ tubes, pseudohyphae, and hyphae, but the genes involved in hyphal growth machinery and virulence remain unclear in c. tropicalis. recently, echinocandin- and azole-resistant c. tropicalis isolates have frequently been isolated from various patients around the world, making ... | 2014 | 24442892 |
| the usefulness of dna sequencing after extraction by whatman fta filter matrix technology and phenotypic tests for differentiation of candida albicans and candida dubliniensis. | since c. dubliniensis is similar to c. albicans phenotypically, it can be misidentified as c. albicans. we aimed to investigate the prevalence of c. dubliniensis among isolates previously identified as c. albicans in our stocks and to compare the phenotypic methods and dna sequencing of d1/d2 region on the ribosomal large subunit (rlsu) gene. a total of 850 isolates included in this study. phenotypic identification was performed based on germ tube formation, chlamydospore production, colony colo ... | 2014 | 24436014 |
| antifungal susceptibility and growth inhibitory response of oral candida species to brucea javanica linn. extract. | candida species have been associated with the emergence of resistant strains towards selected antifungal agents. plant products have been used traditionally as alternative medicine to ease candidal infections. the present study was undertaken to investigate the antifungal susceptibility patterns and growth inhibiting effect of brucea javanica seeds extract against candida species. | 2013 | 24305010 |
| molecular epidemiology, phylogeny and evolution of candida albicans. | a small number of candida species form part of the normal microbial flora of mucosal surfaces in humans and may give rise to opportunistic infections when host defences are impaired. candida albicans is by far the most prevalent commensal and pathogenic candida species. several different molecular typing approaches including multilocus sequence typing, multilocus microsatellite typing and dna fingerprinting using c. albicans-specific repetitive sequence-containing dna probes have yielded a wealt ... | 2014 | 24269341 |
| candida dubliniensis endophthalmitis: five cases over 15 years. | recent studies have shown that the recently identified organism candida dubliniensis is less pathogenic than the more common candida albicans. due to its rare nature, c. dubliniensis has been previously reported as the causative organism in endophthalmitis in only three cases. we undertook a multicenter, retrospective, consecutive case series to describe the clinical features and outcomes of patients with culture-proven c. dubliniensis endophthalmitis. medical records were reviewed for all patie ... | 2013 | 24252588 |
| clinical isolates and laboratory reference candida species and strains have varying abilities to form biofilms. | candida biofilms are a major virulence trait for this yeast. in this study, the biofilm-forming ability of the major medically important clinical and laboratory reference strains was compared. biofilms were quantified using traditional methods, that is, crystal violet (cv), tetrazolium (xtt) reduction and colony-forming unit assays (cfu), and two new methods: an automated cell counter (acc) and biofilm suspension turbidity (bst) method. biofilms could be categorized based on biofilm biomass (hig ... | 2013 | 23927631 |
| an in vitro study on the anti-adherence effect of brucea javanica and piper betle extracts towards oral candida. | the adherence of candida to mucosal surfaces is the initial step for successful invasive process of the oral cavity. the study aimed to investigate the effect of two plant extracts on the non-specific and specific bindings of oral candida. | 2013 | 23915676 |
| comparison of the vitek 2 yeast susceptibility system with clsi microdilution for antifungal susceptibility testing of fluconazole and voriconazole against candida spp., using new clinical breakpoints and epidemiological cutoff values. | a commercially available, fully automated yeast susceptibility test system (vitek 2; biomérieux, marcy d'etoile, france) was compared in 3 different laboratories with the clinical and laboratory standards institute (clsi) reference microdilution (bmd) method by testing 2 quality control strains, 10 reproducibility strains, and 425 isolates of candida spp. against fluconazole and voriconazole. reference clsi bmd mic endpoints and vitek 2 mic endpoints were read after 24 hours and 9.1-27.1 hours i ... | 2013 | 23870164 |
| the prevalence of oral candida infections in periodontitis patients with type 2 diabetes mellitus. | the purpose of this study was to determine the prevalence of candida spp. in periodontitis patients with type 2 diabetes mellitus. | 2013 | 23806705 |
| a stable hybrid containing haploid genomes of two obligate diploid candida species. | candida albicans and candida dubliniensis are diploid, predominantly asexual human-pathogenic yeasts. in this study, we constructed tetraploid (4n) strains of c. albicans of the same or different lineages by spheroplast fusion. induction of chromosome loss in the tetraploid c. albicans generated diploid or near-diploid progeny strains but did not produce any haploid progeny. we also constructed stable heterotetraploid somatic hybrid strains (2n + 2n) of c. albicans and c. dubliniensis by spherop ... | 2013 | 23709179 |
| species and condition specific adaptation of the transcriptional landscapes in candida albicans and candida dubliniensis. | although candida albicans and candida dubliniensis are most closely related, both species behave significantly different with respect to morphogenesis and virulence. in order to gain further insight into the divergent routes for morphogenetic adaptation in both species, we investigated qualitative along with quantitative differences in the transcriptomes of both organisms by cdna deep sequencing. | 2013 | 23547856 |
| efficient neocentromere formation is suppressed by gene conversion to maintain centromere function at native physical chromosomal loci in candida albicans. | cenpa/cse4 assembles centromeric chromatin on diverse dna. cenpa chromatin is epigenetically propagated on unique and different centromere dna sequences in a pathogenic yeast candida albicans. formation of neocentromeres on dna, nonhomologous to native centromeres, indicates a role of non-dna sequence determinants in cenpa deposition. neocentromeres have been shown to form at multiple loci in c. albicans when a native centromere was deleted. however, the process of site selection for cenpa depos ... | 2013 | 23439889 |
| elucidation of a key position for acyltransfer activity in candida parapsilosis lipase/acyltransferase (cplip2) and in pseudozyma antarctica lipase a (cal-a) by rational design. | performing transesterifications in aqueous media is becoming a priority challenge in lipid biotechnology in order to develop more eco-friendly and efficient biocatalytic processes in systems containing both polar and apolar substrates. in this context, our group has explored for several years the high potential of the lipase/acyltransferase cplip2 from candida parapsilosis and of several of its homologs, that catalyze efficiently acyltransfer reactions in lipid/water media with high water activi ... | 2016 | 26602447 |
| interferon-gamma immunotherapy in a patient with refractory disseminated candidiasis. | despite advances in supportive care and novel antifungal agents, mortality caused by invasive candida infection is high. a 3-year-old boy with disseminated candida dubliniensis infection during induction chemotherapy for acute lymphoblastic leukemia deteriorated despite resolution of neutropenia and appropriate antifungal treatment. monocyte human leukocyte antigen-dr expression was extremely low, suggesting immunoparalysis. adjuvant immunotherapy with interferon-gamma restored the immune respon ... | 2015 | 26379166 |
| in vitro postantifungal effect, adhesion traits and haemolysin production of candida dubliniensis isolates following exposure to 5-fluorocytosine. | the phenomenon of postantifungal effect (pafe), which is the suppression of candidal growth following brief exposure to antifungal agents, is linked with candidal pathogenicity. adhesion to buccal epithelial cells (bec), germ tube (gt) formation and relative cell surface hydrophobicity (csh) are all adhesion traits of candidal pathogenicity. ability to produce haemolysin by candida species is also a determinant of its pathogenicity. there is no information on either the pafe or its impact on adh ... | 2015 | 26201447 |
| structure-based functional annotation of hypothetical proteins from candida dubliniensis: a quest for potential drug targets. | candida dubliniensis is an emerging pathogenic yeast in humans and infections are usually restricted to mucosal parts of the body. however, its presence in specimens of immunocompromised individuals, especially in hiv-positive patients, is of major medical concern. there is a large fraction of genomes of c. dubliniensis in the database which are uncharacterized for their biochemical, biophysical, and/or cellular functions, and are identified as hypothetical proteins (hps). function annotation of ... | 2015 | 28324558 |
| in vitro adhesion of oral candida dubliniensis isolates to acrylic denture surfaces following brief exposure to sub-cidal concentrations of polyenes, azoles and chlorhexidine. | we aimed to investigate the effect of brief exposure to sub-cidal concentrations of nystatin, amphotericin b, ketoconazole, fluconazole and chlorhexidine gluconate on the adhesion of oral candida dubliniensis isolates to the surface of acrylic dentures. | 2015 | 25471106 |
| [evaluation of mass spectrometry for the identification of clinically interesting yeasts]. | identification of yeasts is based on morphological, biochemical and nutritional characteristics, and using molecular methods. matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) mass spectrometry, a new method for the identification of microorganisms, has demonstrated to be very useful. the aim of this study is to evaluate this new method in the identification of yeasts. | 2017 | 25444360 |
| impact of brief and sequential exposure to nystatin, amphotericin b, ketoconazole, and fluconazole in modulating adhesion traits of oral candida dubliniensis isolates. | candida adherence is implicated in the pathogenesis of oral candidosis. adhesion to buccal epithelial cells (bec), germ tube (gt) formation, and relative cell surface hydrophobicity (csh) are colonization attributes of candidal pathogenicity. candida dubliniensis (c. dubliniensis) is allied with recurrent oral candidosis, which can be treated with nystatin, amphotericin b, ketoconazole, and fluconazole. due to the diluent effect of saliva and the cleansing effect of the oral musculature in the o ... | 2016 | 25388637 |
| activity of echinocandins and triazoles against a contemporary (2012) worldwide collection of yeast and moulds collected from invasive infections. | in this study, 1717 fungal clinical isolates causing invasive fungal infections were evaluated against nine antifungal agents using clinical and laboratory standards institute (clsi) reference broth microdilution methods. the isolates comprised 1487 candida spp., 109 aspergillus spp., 86 non-candida yeasts (including 52 isolates of cryptococcus neoformans) and 35 rare moulds obtained during 2012 from 72 hospitals worldwide. echinocandin resistance among candida spp. was low, and resistance rates ... | 2014 | 25129315 |
| isavuconazole and nine comparator antifungal susceptibility profiles for common and uncommon candida species collected in 2012: application of new clsi clinical breakpoints and epidemiological cutoff values. | the in vitro activity of isavuconazole and nine antifungal comparator agents was assessed using reference broth microdilution methods against 1,421 common and uncommon species of candida from a 2012 global survey. isolates were identified using chromagar, biochemical methods and sequencing of its and/or 28s regions. candida spp. were classified as either susceptible or resistant and as wild type (wt) or non-wt using clsi clinical breakpoints or epidemiological cutoff values, respectively, for th ... | 2014 | 24952015 |
| uncommon opportunistic yeast bloodstream infections from qatar. | eleven uncommon yeast species that are associated with high mortality rates irrespective of antifungal therapy were isolated from 17/187 (201 episodes) pediatric and elderly patients with fungemia from qatar. the samples were taken over a 6-year period (january 2004-december 2010). isolated species included kluyveromyces marxianus, lodderomyces elongisporus, lindnera fabianii, candida dubliniensis, meyerozyma guilliermondii, candida intermedia, pichia kudriavzevii, yarrowia lipolytica, clavispor ... | 2014 | 24934803 |
| rare case of exogenous candida dubliniensis endophthalmitis: a case report and brief review of the literature. | candida dubliniensis is a recently described opportunistic fungal pathogen that rarely infects the eye. reported cases of c. dubliniensis endophthalmitis have been of endogenous etiology and demonstrated recovery of visual acuity with timely treatment. we herein report an unusual case of severe c. dubliniensis endophthalmitis requiring enucleation. | 2014 | 24860628 |
| post-antifungal effect and adhesion to buccal epithelial cells of oral candida dubliniensis isolates subsequent to limited exposure to amphotericin b, ketoconazole and fluconazole. | the post-antifungal effect (pafe) of candida and its adherence to oral mucosal surfaces are important determinants of candidal pathogenicity. candida dubliniensis is allied with recurrent oral candidosis. oral candidosis can be treated with amphotericin b, ketoconazole and fluconazole. there is no information on the pafe and its impact on adhesion to oral buccal epithelial cells (bec) of oral c. dubliniensis isolates. therefore, the main objective was to reconnoiter the pafe and adhesion to bec ... | 2015 | 24850753 |
| effect of eugenol on cell surface hydrophobicity, adhesion, and biofilm of candida tropicalis and candida dubliniensis isolated from oral cavity of hiv-infected patients. | most candida spp. infections are associated with biofilm formation on host surfaces. cells within these communities display a phenotype resistant to antimicrobials and host defenses, so biofilm-associated infections are difficult to treat, representing a source of reinfections. the present study evaluated the effect of eugenol on the adherence properties and biofilm formation capacity of candida dubliniensis and candida tropicalis isolated from the oral cavity of hiv-infected patients. all isola ... | 2014 | 24799938 |
| high prevalence of candida dubliniensis in lower respiratory tract secretions from cystic fibrosis patients may be related to increased adherence properties. | we identified candida spp isolated from lower respiratory tract secretions obtained from cystic fibrosis (cf) patients, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms), with the aim of determining the most prevalent causative agent. we also sought to determine their adhesive properties in order to understand their biology related to cf. | 2014 | 24780917 |
| functional annotation of putative hypothetical proteins from candida dubliniensis. | an extensive analysis of c. dubliniensis proteomics data showed that ~22% protein are conserved hypothetical proteins (hps) whose function is still not determined precisely. analysis of gene sequence of hps provides a platform to establish sequence-function relationships to a more profound understanding of the molecular machinery of organisms at systems level. here we have combined the latest versions of bioinformatics tools including, protein family, motifs, intrinsic features from the amino ac ... | 2014 | 24704023 |
| candida dubliniensis spondylodiscitis in an immunocompetent patient. case report and review of the literature. | we describe what appears to be the first case of spondylodiscitis due to candida dubliniensis. our case adds to the current literature of the importance of c. dubliniensis as a cause of fungemia and subsequent deep seated infections. it highlights the importance of taking fungal as well as bacterial culture from biopsy specimens in patients with spondylodiscitis. we also review the literature covering the reported cases of candida spondylodiscitis, which amount to about 100 over the last 5 decad ... | 2014 | 24567891 |
| purpurin triggers caspase-independent apoptosis in candida dubliniensis biofilms. | candida dubliniensis is an important human fungal pathogen that causes oral infections in patients with aids and diabetes mellitus. however, c. dubliniensis has been frequently reported in bloodstream infections in clinical settings. like its phylogenetically related virulent species c. albicans, c. dubliniensis is able to grow and switch between yeast form and filamentous form (hyphae) and develops biofilms on both abiotic and biotic surfaces. biofilms are recalcitrant to antifungal therapies a ... | 2013 | 24376900 |
| third case of candida dubliniensis endogenous endophthalmitis in north america: case report and review of the literature. | there are two previous reports of candida dubliniensis endophthalmitis in north america. here, we report a third case of c. dubliniensis endogenous endophthalmitis in a 31-year-old male patient who complained of left-sided decreased visual acuity. he had an associated mitral and tricuspid valve endocarditis, in the setting of intravenous drug use. blood and sputum cultures were positive for c. dubliniensis. fundoscopic examination was consistent with a fungal endophthalmitis. he was treated with ... | 2014 | 24234425 |
| candida dubliniensis encrustation of an obstructing upper renal tract calculus. | we present the case of a 53-year-old man, with a history of alcohol abuse, requiring intensive care unit admission, with an obstructing right upper renal calculus and klebsiella pneumoniae urosepsis. ureteroscopic treatment of this obstruction displayed a small calculus within the renal pelvis completely encapsulated within a fungal bezoar. laboratory analysis of the fungal mass found it to be candida dubliniensis. | 2013 | 23975908 |
| endocarditis caused by candida dubliniensis. | endocarditis caused by candida dubliniensis is a rare event and limited to few case reports. in this report, the authors present a patient with a history of intravenous drug use and hepatitis c and endocarditis involving a prosthetic aortic valve. also reviewed are the treatment guidelines for candida sp. endocarditis. | 2013 | 23974269 |
| the postantifungal effect of nystatin and its impact on adhesion attributes of oral candida dubliniensis isolates. | the postantifungal effect (pafe) has an impact on candidal pathogenicity. however, there is no information on either the pafe or its impact on adhesion traits of oral candida dubliniensis isolates. oral candidosis can be treated topically with nystatin. adhesion to buccal epithelial cells (bec), germ tube (gt) formation and relative cell surface hydrophobicity (csh) are all colonisation attributes of candidal pathogenicity. hence, the main objective of this study was to investigate the in vitro ... | 2014 | 23773155 |
| multicenter study evaluating the vitek ms system for identification of medically important yeasts. | the optimal management of fungal infections is correlated with timely organism identification. matrix-assisted laser desorption ionization-time of flight (maldi-tof) mass spectrometry (ms) is revolutionizing the identification of yeasts isolated from clinical specimens. we present a multicenter study assessing the performance of the vitek ms system (biomérieux) in identifying medically important yeasts. a collection of 852 isolates was tested, including 20 candida species (626 isolates, includin ... | 2013 | 23658267 |
| photodynamic inactivation of yeast and bacteria by extracts of alternanthera brasiliana. | this study was undertaken to evaluate the effect of alternathera brasiliana (amaranthaceae) extracts as photosensitizing agents in photodynamic antimicrobial therapies (pact) against staphylococcus aureus, staphylococcus epidermidis and candida dubliniensis. the crude hexane and ethanol extracts were obtained from a. brasiliana whole plant and showed absortion from 650 to 700 nm. also, singlet molecular oxygen (1o2) production (type ii photosensitization reaction) was examined, and the results s ... | 2013 | 23547779 |
| changes in germ tube formation and cell-surface hydrophobicity of oral candida dubliniensis isolates following brief exposure to sub-cidal concentrations of polyene and azole antifungal agents. | adherence of candida has been implicated as the initial process in the pathogenesis of oral candidosis. candidal germ tubes and its relative cell-surface hydrophobicity (csh) are contributory attributes. candida dubliniensis is currently documented as an opportunistic pathogen allied with recurrent oral candidosis. oral candidosis can be treated with polyene and azole antifungals such as amphotericin b, ketoconazole and fluconazole. however, the intraoral concentration of these drugs fluctuates ... | 2013 | 23405864 |
| the first case of candida dubliniensis endophthalmitis in north america. | | 2013 | 23132215 |
| biological activities of pseudevernia furfuracea (l.) zopf extracts and isolation of the active compounds. | pseudevernia furfuracea (l.) zopf (parmeliaceae) is a common epiphytic lichen in the conifer-hardwood forest of anatolia. this species is used in traditional medicine in turkey as a treatment for wounds, eczema and hemorrhoids. | 2012 | 23107822 |
| the first korean case of candidemia due to candida dubliniensis. | candidemia due to uncommon candida spp. appears to be increasing in incidence. c. dubliniensis has been increasingly recovered from individuals not infected with hiv. identification of c. dubliniensis can be problematic in routine clinical practice due to its phenotypic resemblance to c. albicans. we report the first case of c. dubliniensis candidemia in korea, which occurred in a 64-yr-old woman who presented with partial seizure, drowsiness, and recurrent fever. germ-tube positive yeast that w ... | 2012 | 22563560 |
| cell surface hydrophobicity of oral candida dubliniensis isolates following limited exposure to sub-therapeutic concentrations of chlorhexidine gluconate. | candidal adhesion has been implicated as the initial step in the pathogenesis of oral candidiasis and cell surface hydrophobicity (csh) has been implicated in adhesion to mucosal surfaces. candida dubliniensis is an opportunistic pathogen associated with recurrent oral candidiasis. chlorhexidine gluconate is by far the commonest antiseptic mouth wash prescribed in dentistry. at dosage intervals the intraoral concentration of this antiseptic fluctuates considerably and reaches sub-therapeutic lev ... | 2013 | 22533484 |
| candida dubliniensis: an appraisal of its clinical significance as a bloodstream pathogen. | a nine-year prospective study (2002-2010) on the prevalence of candida dubliniensis among candida bloodstream isolates is presented. the germ tube positive isolates were provisionally identified as c. dubliniensis by presence of fringed and rough colonies on sunflower seed agar. subsequently, their identity was confirmed by vitek2 yeast identification system and/or by amplification and sequencing of the its region of rdna. in all, 368 isolates were identified as c. dubliniensis; 67.1% came from ... | 2012 | 22396802 |
| in vitro activities of antifungal agents alone and in combination against fluconazole-susceptible and -resistant strains of candida dubliniensis. | in the present study we used two groups of candida dubliniensis strains: one containing fluconazole-susceptible clinical isolates and another containing fluconazole-resistant laboratory derivative from the former to examine the changes on susceptibility accompanying the development of resistance to fluconazole. our findings confirmed the ability of c. dubliniensis isolates to become resistant to fluconazole and indicated that this resistance was crossed with ketoconazole, itraconazole, ravuconaz ... | 2017 | 22358361 |
| candida dubliniensis endophthalmitis: first case in north america. | to report an unusual case of endogenous fungal endophthalmitis due to candida dubliniensis. interventional case report of a 27-year-old immunocompetent male with loss of vision, dense vitritis, and chorioretinal infiltrates, who underwent a diagnostic pars plana vitrectomy. microbiology cultures obtained by a diagnostic vitrectomy were positive for the growth of c. dubliniensis. this infectious process was then appropriately treated with intravitreal amphotericin b and systemic fluconazole with ... | 2012 | 22222717 |
| evaluation of the in vitro activity of isavuconazole and comparator voriconazole against 2635 contemporary clinical candida and aspergillus isolates. | the in vitro activity of isavuconazole was determined for 1677 candida and 958 aspergillus isolates from 2012 to 2014 with voriconazole as comparator. | 2017 | 28373148 |
| prevalence and antifungal susceptibility of candida species in a tertiary care hospital in islamabad, pakistan. | to determine the prevalence and antifungal susceptibility pattern of candida species. | 2017 | 28770873 |
| anticandidal activity of extracts and a novel compound, amnomopin, isolated from petriella setifera. | a novel triterpenoidal compound named 'amnomopin' (3β-diglucoside-5,12-28-oic acid), which is named iupac as 3-o-(2' ➔ 1″diglucoside)1,2,3,4,4a,5,6,6a,6b,7,9,10,11,12,12a,12b,13,14b-octadecahydro-10-hydroxy-2,2,6a,6b,9,9,12a-heptamethylpicene-4a-carboxylic acid, was isolated from the extract petriella setifera. the total alcoholic extract of p. setifera showed a great activity against clinically isolated candida species, including candida albicans, candida dubliniensis, candida famata, candida g ... | 2017 | 28730719 |
| exoenzyme activity and possibility identification of candida dubliniensis among candida albicans species isolated from vaginal candidiasis. | vulvovaginal candidiasis (vvc) or vaginal candidiasis is a common fungal infection of the genitals causing inflammation, irritation, itching, and vaginal discharge. common yeast infections are caused by the yeast species c. albicans. however, there are other species of candida such as c. dubliniensis which are considered as the causative agents of this infection. hydrolytic enzymes such as proteinase and coagulase are known as virulence factors. the aim of this study was the molecular confirmati ... | 2017 | 28642006 |
| oral mycobiome in community-dwelling elderly and its relation to oral and general health conditions. | oral fungal infection is generally associated with dysbiosis related to antibiotic use, immunodeficiency, or frailty. however, fungal colonization in a typical population without apparent symptoms and its associated conditions are poorly understood. in this study, oral fungal colonization in community-dwelling and independently living elderly populations was evaluated and factors affecting fungal colonization were analyzed. | 2017 | 28419681 |
| persistence of candida dubliniensis and lung function in patients with cystic fibrosis. | candida dubliniensis is an emerging yeast and demonstrated a high adherence property to cystic fibrosis respiratory tract. therefore, it is important to determine the persistence of c. dubliniensis and to assess the possible relationship to the body mass index (bmi) and forced expiratory volume in 1st second (fev1). | 2017 | 28747217 |
| fungi of the genus rhodotorula isolated from the oral cavity of oncologic patients with colorectal cancer | this paper concerns the frequency of occurrence and enzymatic activity of fungi of the genus rhodotorula isolated from the oral cavity of patients with colorectal cancer. swabs from the oral cavity were subjected to standard mycological diagnostic procedures; enzymatic activity of the fungi was evaluated using and api zym test by biomereux. the fungi of the genus rhodotorula were noted in 22.5% positive isolates originating from women and 25.7% positive isolates originating from men. they includ ... | 2017 | 28741338 |
| prevalence of candida africana and candida dubliniensis, in vulvovaginal candidiasis: first turkish candida africana isolates from vulvovaginal candidiasis. | candida africana and c. dubliniensis are closely related species of c. albicans. current phenotypic methods are not suitable to accurately distinguish all the species belonging to the c. albicans complex. several molecular-based methods have recently been designed for discriminating among closely related candida species. the aim of this study was to establish the prevalence of c. dubliniensis and c. africana in vulvovaginal samples with phenotypic and genotypic methods. | 2017 | 28641919 |
| comparative evaluation of six chromogenic media for presumptive yeast identification. | the present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. | 2017 | 28663328 |
| molecular identification and in-vitro antifungal susceptibility testing of candida species isolated from patients with onychomycosis. | candida species are the most opportunistic fungi affecting the nails and resulting in onychomycosis. in this study, we identified and evaluated in-vitro susceptibility of the recovered isolates against fluconazole (flc), voriconazole (vrc), and clotrimazole (clt) using the clinical and laboratory standards institute (clsi) m27-a3 document. | 2015 | 28681001 |
| phenotypic and genotypic detection of candida albicans and candida dubliniensis strains isolated from oral mucosa of aids pediatric patients. | the aim of this study was to assess a collection of yeasts to verify the presence of candida dubliniensis among strains isolated from the oral mucosa of aids pediatric patients which were initially characterized as candida albicans by the traditional phenotypic method, as well as to evaluate the main phenotypic methods used in the discrimination between the two species and confirm the identification through genotypic techniques, i.e., dna sequencing. twenty-nine samples of c. albicans isolated f ... | 2017 | 28423089 |
| methods of candida dubliniensis identification and its occurrence in human clinical material. | candida dubliniensis was reported as a new species in 1995. this species is often misidentified as candida albicans. the aims of this work were to determine the occurrence of c. dubliniensis in various clinical materials, to evaluate several ways to identify it and to examine the genetic variability of isolates. among 7706 isolates originally identified as c. albicans, 237 were identified as c. dubliniensis (3.1%). most of the c. dubliniensis isolates were obtained from the upper and lower respi ... | 2017 | 28516267 |
| usefulness of the non-conventional caenorhabditis elegans model to assess candida virulence. | invasive candidiasis is caused mainly by candida albicans, but other candida species have increasing etiologies. these species show different virulence and susceptibility levels to antifungal drugs. the aims of this study were to evaluate the usefulness of the non-conventional model caenorhabditis elegans to assess the in vivo virulence of seven different candida species and to compare the virulence in vivo with the in vitro production of proteinases and phospholipases, hemolytic activity and bi ... | 2017 | 28523422 |
| denaturing gradient gel electrophoresis profiles of bacteria from the saliva of twenty four different individuals form clusters that showed no relationship to the yeasts present. | the aim was to investigate the relationship between groups of bacteria identified by cluster analysis of the dgge fingerprints and the amounts and diversity of yeast present. | 2017 | 28577425 |
| comparison of duplex pcr and phenotypic analysis in differentiating candida dubliniensis from candida albicans from oral samples. | candida dubliniensis shares a wide range of phenotypic characteristics with candida albicans including a common trait called germ tube positivity. hence, laboratory differentiation of these two species is cumbersome. duplex pcr analyses for c. albicans and c. dubliniensis was performed directly on dna extracted from a total of 122 germ tube positive isolates derived from 100 concentrated oral rinse samples from a random cohort of diabetics attending a clinic in sri lanka. these results were conf ... | 2017 | 28658945 |
| killing activity of micafungin against candida albicans, c. dubliniensis and candida africana in the presence of human serum. | we compared killing activity of micafungin in time-kill experiments in rpmi-1640 with and without 50% serum against candida albicans, candida dubliniensis and candida africana reference strains and clinical isolates. killing rates (k values) were determined for each strain and concentration. in rpmi-1640 mic ranges were 0.015-0.03, 0.015-0.03 and 0.015 mg/l against c. albicans, c. dubliniensis and c. africana, respectively. in 50% serum mic values for the three species increased 16- to 64-fold. ... | 2017 | 28699056 |