| molecular identification of mycoplasma synoviae from breeder chicken flock showing arthritis in egypt. | arthritis is one of the most economic problems facing poultry industry worldwide. the study was done to detect possible causes of arthritis in breeder chicken flock with emphasis on molecular identification of mycoplasma synoviae (ms). | 2019 | 31190708 |
| autophagy induced by avian reovirus enhances viral replication in chickens at the early stage of infection. | avian reovirus (arv) is an important pathogen that can cause serious disease in poultry. though several in vitro studies revealed some molecular mechanisms that are responsible for arv-induced autophagy, it is still largely unknown how arv manipulates autophagy to promote its own propagation. | 2019 | 31126305 |
| a two-year prospective study of small poultry flocks in ontario, canada, part 1: prevalence of viral and bacterial pathogens. | in ontario, within the past few years, there has been a marked increase in the number of non-commercial poultry flocks (referred to as "small flocks"). small poultry flocks may act as a reservoir of avian and zoonotic pathogens, given the flocks' limited access to veterinary services, inadequate biosecurity practices, and increased risk of contact with wild birds. despite these potential risks, there is a scarcity of data concerning the prevalence of poultry and zoonotic pathogens among these fl ... | 2019 | 30973091 |
| occurrence of reovirus (arv) infections in poultry flocks in poland in 2010-2017. | avian reovirus (arv) infections in poultry populations are reported worldwide. the reovirus belongs to the genus orthoreovirus, family reoviridae. the aim of the study was to evaluate the incidence of arv infections in the poultry population based on diagnostic tests performed in 2010-2017. | 2018 | 30729197 |
| sensitive sybr green-real time pcr for the detection and quantitation of avian rotavirus a. | avian rotavirus a (artv-a) is a virus that affects young birds, causing acute diarrhea and economic losses in the poultry industry worldwide. the techniques used for the diagnosis of artv-a include electron microscopy, isolation in cell culture, and serology, as well as molecular techniques, such as the reverse transcription-polymerase chain reaction (rt-pcr). the objective of this work was to standardize a real-time rt-polymerase chain reaction (rt-qpcr) using sybr green chemistry for the rapid ... | 2018 | 30597964 |
| isolation and genomic characterization of a novel chicken-orign orthoreovirus causing goslings hepatitis. | a severe infectious disease characterized by nephritis, hepatitis and splenitis has attacked goslings around shandong province in china since 2016. a novel chicken-origin avian orthoreovirus (arv) was isolated with lmh cells from affected goslings named reo/goose/sdpy/1116/17 (sdpy-arv) strain, and the infection was successfully reproduced experimentally. the arv-sdpy full genome sequencing was conducted using next-generation sequencing (ngs) technique on illumina hiseq platform. the complete ge ... | 2018 | 30473354 |
| a multiplex xtag assay for the simultaneous detection of five chicken immunosuppressive viruses. | chicken anemia virus (cav), avian reovirus (arv), infectious bursal disease virus (ibdv), marek's disease virus (mdv) and reticuloendotheliosis virus (rev) all cause immunosuppressive disease in birds through vertical or horizontal transmission. mixed infections with these immunosuppressive pathogens lead to atypical clinical signs and obstruct accurate diagnoses and epidemiological investigations. therefore, it is essential to develop a high-throughput assay for the simultaneous detection of th ... | 2018 | 30442149 |
| molecular characterization of emerging avian reovirus variants isolated from viral arthritis cases in western canada 2012-2017 based on partial sigma (σ)c gene. | viral arthritis (va), a disease caused by avian reovirus (arv), has emerged as a significant cause of economic losses in broiler chicken flocks in western canada. these outbreaks were characterized by 4-13% morbidity, followed by a spike in mortality/culling that in extreme cases required total flock depopulation. from 2012-2017, 38 arv isolates were recovered. molecular characterization of a partial segment of the sigma (σ)c gene shows all six previously known arv clusters in western canadian b ... | 2018 | 30029013 |
| in vitro study on role of σb protein in avian reovirus pathogenesis. | avian reoviruses, members of orthoreovirus genus was known to cause diseases like tenosynovitis, runting-stunting syndrome in chickens. among eight structural proteins, the proteins of s-class are mainly associated with viral arthritis but the significance of σb protein in arthritis is not established till date. in this infection pathological condition together with infection of joints often leads to arthritis because joints consists of cartilage which forms lubricating surface between two bones ... | 2018 | 29731966 |
| development of a recombinant σb protein based dot-elisa for the diagnosis of avian reovirus (arv). | avian reovirus (arv) causes significant economic losses to the poultry industry worldwide. the arv proteins fall into three different classes based on their sizes:λ (large); μ (medium) and σ (small). σb, an outer capsid protein of the arv contains group specific neutralizing epitopes and induces strong immune response in naturally infected chickens. this study describes the development of a rapid dot-enzyme linked immunosorbent assay (dot-elisa) using recombinant σb protein antigen of 54 kda (ap ... | 2018 | 29660384 |
| a novel orthoreovirus associated with epizootic necrotizing enteritis and splenic necrosis in american crows (corvus brachyrhynchos). | epizootic mortalities in american crows (corvus brachyrhynchos) during the winter months, referred to as winter mortality of crows, have been recorded in north america for almost two decades. the most common postmortem findings include necrotizing enteritis, colitis, and fibrinous splenic necrosis. these findings are proposed to be due to infection with a reovirus sp. our objectives were to characterize the pathology and seasonality of the epizootics in new york state (nys), confirm the causativ ... | 2019 | 31107635 |
| genomic characterization of avian and neoavian orthoreoviruses detected in pheasants. | avian reoviruses are well-known pathogens seriously affecting the productivity of poultry industry. game birds represent a small segment of the agricultural sector and much remained to be learnt about factors affecting productivity. here we show that reovirus infections might occur in pheasants and demonstrate that reoviruses of pheasants are of diverse origin. the complete or coding-complete genomic sequences of two hungarian reovirus strains, d1996/2/1 and reo/hun/pheasant/216/2015, have been ... | 2021 | 33631220 |
| dynamic changes in the expression of interferon-stimulated genes in joints of spf chickens infected with avian reovirus. | avian reovirus (arv) can induce many diseases as well as immunosuppression in chickens, severely endangering the poultry industry. interferons (ifns) play an antiviral role by inducing the expression of interferon-stimulated genes (isgs). the effect of arv infection on the expression of host isgs is unclear. specific-pathogen-free (spf) chickens were infected with arv strain s1133 in this study, and real time quantitative pcr was used to detect changes in the dynamic expression of ifns and commo ... | 2021 | 33614762 |
| emerging new avian reovirus variants from cases of enteric disorders and arthritis/tenosynovitis in brazilian poultry flocks. | 1. the objective of this study was to characterise circulating brazilian avian reovirus (arv) strains by genetic analysis of the σc protein encoded by segment 1 of the viral genome and compare these with those of viral strains used for immunising commercial poultry.2. the analysis detected the presence of arv genomes by quantitative reverse transcriptase pcr (rt-qpcr) in the enteric samples and the joint tissues (jt) of birds with signs of viral arthritis/tenosynovitis. nucleotide sequencing use ... | 2021 | 33448227 |
| gallus nme/nm23 nucleoside diphosphate kinase 2 interacts with viral σa and affects the replication of avian reovirus. | our present study aimed to identify host cell proteins that may interact with avian reovirus (arv) σa protein and their potential effect on arv replication. the arv structural protein σa has been demonstrated to suppress interferon production and confirmed to activate the pi3k/akt pathway. however, host cell factors interacting with σa to affect arv replication remain unknown. in current study, a cdna library of chicken embryo fibroblasts (cefs) was constructed, and host cell proteins interactin ... | 2020 | 33223234 |
| establishment of a real-time recombinase polymerase amplification assay for the detection of avian reovirus. | avian reovirus (arv) infection results in multiple disease manifestations in chicken. a rapid detection method will contribute to early diagnosis and control of the virus infection. the recombinase polymerase amplification (rpa) technology is a nucleic acid amplification method which is experiencing rapid development. in present study, a real-time reverse transcription (rt)-rpa assay was developed for the detection of arv. the limit of detection of the real-time rt-rpa was 102 copies/μl of arv g ... | 2020 | 33195523 |
| new insights on avian orthoreovirus and chicken astrovirus co-infection in an italian broiler flock: preliminary biomolecular and pathological results. | common pathogens of intensive poultry farms, either parasitic or bacterial, such as coccidiaor salmonella, are well known and strictly controlled by veterinary management. this case study reports an unusual case of runting stunting syndrome (rss) observed on a sicilian poultry farm of broiler chickens during 2019. the investigation was carried out on five chickens which present delayed in body weight and growth performance. animals showed also difficulty in deambulation and diarrhea. at necropsy ... | 2021 | 34313103 |
| metagenomic characterisation of additional and novel avian viruses from australian wild ducks. | birds, notably wild ducks, are reservoirs of pathogenic and zoonotic viruses such as influenza viruses and coronaviruses. in the current study, we used metagenomics to detect and characterise avian dna and rna viruses from wild pacific black ducks, chestnut teals and grey teals collected at different time points from a single location. we characterised a likely new species of duck aviadenovirus and a novel duck gyrovirus. we also report what, to the best of our knowledge, is the first finding of ... | 2020 | 33335272 |
| detection and characterization of chicken astrovirus associated with hatchery disease in commercial day-old turkeys in southwestern nigeria. | infectious diseases are a major obstacle to profitable poultry production in nigeria due to the mortality and severe economic losses they cause. in particular, they are a potent threat to attainment of the food security goals of government and national self-sufficiency in food production. thus, there is a need for continuous monitoring of the nation's poultry population for these diseases. as part of an ongoing investigation of enteric viruses associated with poor performance or hatchery disease ... | 2021 | 33759002 |