| enhanced host defense after gene transfer in the murine p47phox-deficient model of chronic granulomatous disease. | the p47phox-/- mouse exhibits a phenotype similar to that of human chronic granulomatous disease (cgd) and, thus, is an excellent model for the study of gene transfer technology. using the moloney murine leukemia virus-based retroviral vector mfg-s encoding the human form of p47phox, we performed ex vivo gene transfer into sca-1+ p47phox-/- marrow progenitor cells without conditioning of donors with 5-fluorouracil. transduced progenitors were transplanted into moderately irradiated (500 cgy), g- ... | 1997 | 9116268 |
| melioidosis in india: the tip of the iceberg? | diagnosis of melioidosis by the isolation of burkholderia pseudomallei from one or more body fluid/tissue specimens of 6 indian subjects, 5 of whom had not travelled outside india, is reported. the places of residence of these 6 and one patient previously reported, namely tripura (2), kerala (2), orissa (1), tamil nadu (1) and maharashtra (1) are therefore potentially endemic for melioidosis. b.pseudomallei closely resembles common contaminant pseudomonas sp. and are easily mis-identified in mic ... | 1996 | 8926030 |
| comparative in vitro activity of meropenem versus other extended-spectrum antimicrobials against randomly chosen and selected resistant clinical isolates tested in 26 north american centers. | the in vitro antibacterial activity of meropenem and up to nine other antimicrobials was compared in studies at 26 north american centers from 1989 to 1992 with use of standardized and controlled procedures for determining minimal inhibitory concentrations (mics) against 12,483 recent clinical isolates and additional drug-resistant strains. overall, carbapenems were the most active drugs. the antibacterial activity of meropenem was consistent against random isolates in all centers; however, incl ... | 1997 | 9126699 |
| nosocomial outbreak of burkholderia pickettii infection due to a manufactured intravenous product used in three hospitals. | forty-six cases of nosocomial infection caused by burkholderia pickettii were reported between june and november 1993 in three metropolitan hospitals in madrid. a case-control study of the outbreak was conducted to identify its cause. seventy-four percent of the patients were males; the mean age +/- sd of the patients was 54 +/- 20 years. sixty-five percent of the patients presented with some gastrointestinal disorder, and 80% had a peripheral catheter; 98% were treated with intravenous fluids, ... | 1996 | 8783718 |
| characterization of burkholderia pseudomallei and burkholderia pseudomallei-like strains. | previous reports in the literature suggest that burkholderia pseudomallei strains can be differentiated on the basis of animal virulence. twenty environmentally and clinically derived isolates of burkholderia pseudomallei were examined for the production of exoenzymes, morphological and biochemical phenotypes and virulence for syrian golden hamsters. the partial sequence of the 16s ribosomal rna [rrna] genes from a number of these strains was also determined. based upon these observations, it is ... | 1997 | 9129590 |
| nosocomial burkholderia cepacia outbreaks and pseudo-outbreaks. | | 1996 | 8934237 |
| geographical distribution of pseudomonas pseudomallei in china. | 1,366 samples of soil and water from southern china coastal provinces were examined for pseudomonas pseudomallei. data showed that 58 samples were positive for this bacteria, which is primarily distributed in hainan province and the coastal region of mainland. this paper confirmed the environmental presence of p. pseudomallei in china and showed that the distribution of this pathogen has at least reached a latitude of 25.5 degrees north. | 1995 | 9139366 |
| burkholderia pseudomallei infection in the singapore armed forces from 1987 to 1994--an epidemiological review. | between 1987 and 1994, twenty-three cases of burkholderia pseudomallei infection (melioidosis) were diagnosed in persons serving in the singapore armed forces. there were four deaths resulting from complications of the infection. unlike the situation in the general population, where the affected are mainly the elderly with underlying illness, the majority of cases in the singapore armed forces were otherwise fit and healthy young servicemen. serological surveys have shown the prevalence of the i ... | 1997 | 9140571 |
| isolation of an acanthamoeba strain with intracellular burkholderia pickettii infection. | burkholderia pickettii is a facultative pathogen that has been isolated from patient sources and environmental sources including respiratory therapy solutions, deionized water and aqueous disinfectants. the organism has been associated with septicemia and respiratory tract infections. in our investigation, burkholderia pickettii (biovar 2) was for the first time isolated from acanthamoeba sp. (group ii), a free living amoeba species recovered from the wet area of a physiotherapy unit. pathogenic ... | 1997 | 9144916 |
| antibacterial activity of bms-180680, a new catechol-containing monobactam. | the in vitro activities of a new catechol-containing monobactam, bms-180680 (sq 84,100), were compared to those of aztreonam, ceftazidime, imipenem, piperacillin-tazobactam, ciprofloxacin, amikacin, and trimethoprim-sulfamethoxazole. bms-180680 was often the most active compound against many species of the family enterobacteriaceae, with mics at which 90% of the isolates were inhibited (mic90s) of < or = 0.5 microg/ml for escherichia coli, klebsiella spp., citrobacter diversus, enterobacter aero ... | 1997 | 9145861 |
| septicemia and suppuration in a vietnam veteran. | | 1997 | 9153149 |
| three-year outbreak of pseudobacteremia with burkholderia cepacia traced to a contaminated blood gas analyzer. | between november 1990 and june 1993, burkholderia cepacia was isolated from the blood cultures of 13 neonates born at the ottawa general hospital. eight of the 13 neonates appeared symptomatic, and only 4 were treated with appropriate antimicrobial therapy, but all improved clinically. in august 1993, the blood gas analyzer in the neonatal intensive-care unit was found to be contaminated heavily with b cepacia. eight available patient isolates were identical to the isolates recovered from the bl ... | 1996 | 8934241 |
| an outbreak of burkholderia cepacia lower respiratory tract infection associated with contaminated albuterol nebulization solution. | an outbreak of burkholderia cepacia lower respiratory tract colonization and infection occurred in the adult intensive-care units in various geographic locations throughout our hospital. forty-four patients became colonized or infected over an 11-month period, whereas b cepacia had been isolated from only 13 patients in the preceding 48 months. environmental cultures revealed the source to be extrinsically contaminated albuterol nebulization solution. polymerase chain reaction-ribotyping confirm ... | 1996 | 8934242 |
| epidemiology of pulmonary colonization with burkholderia cepacia in cystic fibrosis patients. the french observatoire burkholderia cepacia study group. | | 1996 | 8950569 |
| comparison of biotyping, ribotyping, and pulsed-field gel electrophoresis for investigation of a common-source outbreak of burkholderia pickettii bacteremia. | over a 3-month period, six immunocompromised patients developed one or more episodes of burkholderia pickettii bacteremia and/or catheter infection. vials of a commercially available, "sterile" saline for injection which had been used for flushing the patients' indwelling intravenous devices were implicated as the common source of the organisms. no further cases were diagnosed once the use of this saline was discontinued. twenty-six isolates, including 9 outbreak-related strains from case patien ... | 1997 | 9163452 |
| cloning, characterization, and sequence analysis of the clce gene encoding the maleylacetate reductase of pseudomonas sp. strain b13. | a 3,167-bp psti fragment of genomic dna from pseudomonas sp. strain b13 was cloned and sequenced. the gene clce consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 da which showed maleylacetate reductase activity. the protein had significant sequence similarities with the polypeptides encoded by tcbf of pp51 (59.4% identical positions), tfdf of pjp4 (55.1%), and tfte of burkholderia cepacia ac1100 (53.1%). the function of tcbf as maleylacetate red ... | 1997 | 9171435 |
| four genes from pseudomonas fluorescens that encode the biosynthesis of pyrrolnitrin. | pyrrolnitrin is a secondary metabolite of pseudomonas and burkholderia sp. strains with strong antifungal activity. production of pyrrolnitrin has been correlated with the ability of some bacteria to control plant diseases caused by fungal pathogens, including the damping-off pathogen rhizoctonia solani. pseudomonas fluorescens bl915 has been reported to produce pyrrolnitrin and to be an effective biocontrol agent for this pathogen. we have isolated a 32-kb genomic dna fragment from this strain ... | 1997 | 9172332 |
| evaluation of contamination of the dentist and dental surgery environment with burkholderia (pseudomonas) cepacia during treatment of children with cystic fibrosis. | pulmonary colonization with burkholderia (pseudomonas) cepacia in cystic fibrosis (cf) patients has been associated with high morbidity and mortality. the aim of this study was to investigate whether the dentist and the dental surgery environment become contaminated during treatment of cf patients colonized with b. cepacia. extensive environmental sampling was carried out during aerosol-generating treatment sessions on four cf patients whose sputum was persistently colonized with b. cepacia. sam ... | 1995 | 8957838 |
| structure of catechol 2,3-dioxygenase gene encoded in tom plasmid of pseudomonas cepacia g4. | the catechol 2,3-dioxygenase is an extradiol-type dioxygenase which cleaves the c-c bond at the meta position of catechol to form 2-hydroxymuconic semialdehyde. a catechol 2,3-dioxygenase gene (tomb) in the tom plasmid of p. cepacia g4 has been cloned and its nucleotide sequence was analyzed. the enzyme gene consisted of 945 base pairs with an atg initiation codon and a tga termination codon which can encode a polypeptide of molecular weight 35 kda containing 314 amino acid residues, and a putat ... | 1997 | 9175755 |
| two patients with recurrent melioidosis after prolonged antibiotic therapy. | melioidosis is a tropical infectious disease caused by burkholderia (pseudomonas) pseudomalleï. clinically manifest melioidosis occurs mostly in people with underlying disorders. melioidosis is a disease with protein manifestations and a high rate of relapse. two patients with infection due to burkholderia pseudomalleï after a visit to thailand are described. both patients presented with sepsis and appropriate therapy was initiated and continued for several months. despite such long-term antimic ... | 1997 | 9181661 |
| in-vitro activity of carbapenem antibiotics against beta-lactam susceptible and resistant strains of burkholderia pseudomallei. | four carbapenem antibiotics were tested for their in-vitro activities (mics, mbcs and time-kill studies) against burkholderia pseudomallei. the carbapenems were all more active than either ceftazidime or co-amoxiclav against strains of b. pseudomallei with a normal susceptibility pattern. biapenem was the most active antibiotic tested. all four carbapenems retained bactericidal activity against 24 strains of b. pseudomallei with reduced susceptibility to ceftazidime and/or co-amoxiclav. | 1996 | 9182118 |
| affinity and response of burkholderia pseudomallei and burkholderia cepacia to insulin. | the cells of burkholderia pseudomallei, b. cepacia and pseudomonas aeruginosa grown on agar plates were stained with fluorescently-labeled insulin. the former two species were stained positively indicating insulin binding but p. aeruginosa was not. insulin exposure reduced phospholipase c and acid phosphatase activities of b. pseudomallei but did not affect those enzymatic activities of b. cepacia in the employed experimental conditions. it is suggested that b. pseudomallei have insulin receptor ... | 1996 | 9185275 |
| evolution of cell-surface acid phosphatase of burkholderia pseudomallei. | acid phosphatase active fractions were obtained from cell-free extract, outermembrane fraction and culture filtrate of burkholderia pseudomallei by column chromatography with sepharose 6b and deae cellulose. the comparison of the elution patterns of protein, sugar and enzymatic activity among these three components suggested that the enzyme is a glycoprotein evolving from premature proteins through glycosylation and that the enzyme is translocated during glycosylation from the cytoplasm to the o ... | 1996 | 9185276 |
| phylogenetic and physiological comparisons of pah-degrading bacteria from geographically diverse soils. | the diversity of bacteria isolated from creosote- contaminated soils in the united states, norway, and germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (pahs), their phospholipid ester-linked fatty acid (gc-fame) profiles, sole carbon source utilization patterns (biolog assays), and 16s rrna sequences. bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. many were capable of degrading a broad range of the pahs found in cr ... | 1997 | 9195008 |
| extradural hematoma following maxillary sinusitis. case illustration. | | 1997 | 9202282 |
| burkholderia cepacia: medical, taxonomic and ecological issues. | the increasing challenge posed by multiresistant saprophytes in medical microbiology is strikingly demonstrated by the emergence of burkholderia (formerly pseudomonas) cepacia as an opportunist pathogen in immunocompromised patients, particularly individuals with chronic granulomatous disease and cystic fibrosis (cf). best known previously as a phytopathogen and the cause of soft rot of onions, b. cepacia presents three major problems for the cf community: innate multiresistance to antimicrobial ... | 1996 | 8958242 |
| caesarean section in a parturient with respiratory failure caused by cystic fibrosis. | we describe a 27-year-old primigravida suffering from cystic fibrosis. her chest was colonised with burkholderia cepacia and she was in respiratory failure for which she required constant nasal intermittent positive pressure ventilation. in view of her rapid deterioration, caesarean section was performed under epidural anaesthesia at 25 weeks gestation. a live 790-g boy was delivered. post-operatively she made steady progress for 5 days although still requiring nasal ventilatory support. thereaf ... | 1997 | 9203887 |
| subdural empyema due to burkholderia cepacia: an unusual complication after lung transplantation for cystic fibrosis. | | 1997 | 9204013 |
| biochemical characteristics of clinical and environmental isolates of burkholderia pseudomallei. | the biochemical characteristics of 213 isolates of burkholderia pseudomallei from patients with melioidosis and 140 isolates from the soil in central and northeastern thailand were compared. whereas the biochemical profiles of all the clinical isolates were similar, all soil isolates from the central area and 25% of isolates from northeastern thailand comprised a different phenotype. this was characterised by the ability to assimilate l-arabinose (100%), adonitol (100%), 5-keto-gluconate (90%) a ... | 1996 | 8958243 |
| comparative activity of trovafloxacin, alone and in combination with other agents, against gram-negative nonfermentative rods. | in the first part of this study, agar dilution mics were used to test the activities of trovafloxacin, ciprofloxacin, ofloxacin, levofloxacin, sparfloxacin, clinafloxacin, ceftazidime, and imipenem against 458 gram-negative nonfermenters. the overall respective mics at which 50% of isolates are inhibited (mic50s) and mic90s were as follows: trovafloxacin, 1.0 and 16.0 microg/ml; ciprofloxacin, 2.0 and 16.0 microg/ml; ofloxacin, 2.0 and 32.0 microg/ml; levofloxacin, 1.0 and 16.0 microg/ml; sparfl ... | 1997 | 9210669 |
| production of specific monoclonal antibodies to burkholderia pseudomallei and their diagnostic application. | hybridomas secreting monoclonal antibodies (mabs) specific to burkholderia pseudomallei were produced by immunizing balb/cj mice with crude culture filtrate of b. pseudomallei. two monoclonal antibodies were found to be highly specific to b.pseudomallei as tested by indirect enzyme-linked immunosorbent assay and immunoblotting against a panel of crude whole cell extracts from b. pseudomallei, b. cepacia, pseudomonas aeruginosa, p.putida, and escherichia coli. one of the specific mabs, clone sp-m ... | 1996 | 8980799 |
| porin from the halophilic species ectothiorhodospira vacuolata: cloning, structure of the gene and comparison with other porins. | the gene coding for the anion-specific porin of the halophilic eubacterium ectothiorhodospira (ect.) vacuolata was cloned and sequenced, the first such gene so analyzed from a purple sulfur bacterium. it encodes a precursor protein consisting of 374 amino acid (aa)-residues including a signal peptide of 22-aa residues. comparison with aa sequences of porins from several other members of the proteobacteria revealed little homology. only two regions showed local homology with the previously sequen ... | 1997 | 9218724 |
| bacterial colonization as a potential source of nosocomial respiratory infections in two types of spirometer. | the potential risk of spirometers in the transmission of respiratory infections has not been yet established. we performed a prospective cross-sectional study to determine the rate of colonization of a water-sealed spirometer and a pneumotachograph, and the potential risk of cross-transmission of microorganisms to patients using each of these devices. fifty four patients (aged 51 +/- 18 (mean +/- sd) yrs) were included in the study. all of them had undergone forced spirometry with bronchodilator ... | 1996 | 8980977 |
| trichloroethylene oxidation by purified toluene 2-monooxygenase: products, kinetics, and turnover-dependent inactivation. | trichloroethylene is oxidized by several types of nonspecific bacterial oxygenases. toluene 2-monooxygenase from burkholderia cepacia g4 is implicated in trichloroethylene oxidation and is uniquely suggested to be resistant to turnover-dependent inactivation in vivo. in this work, the oxidation of trichloroethylene was studied with purified toluene 2-monooxygenase. all three purified toluene 2-monooxygenase protein components and nadh were required to reconstitute full trichloroethylene oxidatio ... | 1997 | 8981984 |
| 2-naphthoate catabolic pathway in burkholderia strain jt 1500. | burkholderia strain (jt 1500), able to use 2-naphthoate as the sole source of carbon, was isolated from soil. on the basis of growth characteristics, oxygen uptake experiments, enzyme assays, and detection of intermediates, a degradation pathway of 2-naphthoate is proposed. the features of this pathway are convergent with those for phenanthrene. we propose a pathway for the conversion of 2-naphthoate to 1 mol (each) of pyruvate, succinate, and acetyl coenzyme a and 2 mol of co2. during growth in ... | 1997 | 8981987 |
| structure of the o-specific polysaccharide from burkholderia vietnamiensis strain lmg 6998. | the putative o-specific polymer containing d-mannose and l-rhamnose was isolated from the lipopolysaccharide obtained from cells walls of burkholderia vietnamiensis strain lmg 6998. nmr and degradative studies showed that the polymer has a linear trisaccharide repeating-unit of the structure shown. the same polymer carrying an o-acetyl group at position 3 of the 4-substituted mannose residue has previously been found as the o antigen in the related species burkholderia cepacia serogroup j. [form ... | 1996 | 9002192 |
| ribotype differences between clinical and environmental isolates of burkholderia pseudomallei. | burkholderia pseudomallei is isolated frequently from the soil in regions where the disease melioidosis occurs. however, recent surveys in thailand have shown that the frequency of isolation of the organism from soil samples is not directly related to the incidence of melioidosis in an area. to determine whether strain populations of b. pseudomallei prevalent in soil are gentypically related to strains causing clinical disease, rrna bamhi restriction fragment length polymorphisms (rflp) of 139 s ... | 1997 | 9236740 |
| the clonal antibody response to pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients. | the groel protein of pseudomonas aeruginosa belongs to the bacterial 60-65 kda heat shock protein family. a strong antibody response to groel has been found in cystic fibrosis (cf) patients with chronic pulmonary infection caused by p. aeruginosa. clonotypes of igg1 and igg2 antibodies against groel were studied in 60 consecutive sera from 18 cf patients with chronic p. aeruginosa infection using isoelectric focusing in combination with affinity immunoblotting. the persistent antigenic stimulati ... | 1997 | 9236862 |
| activity of pseudomonas cepacia lipase in organic media is greatly enhanced after immobilization on a polypropylene support. | the purified lipase from pseudomonas cepacia was used as free and immobilized enzyme preparation for hydrolysis of p-nitrophenyl palmitate (pnpp) and p-nitrophenyl acetate (pnpa) in organic media. the free enzyme was mixed with bovine serum albumin and lyophilized. immobilization was on porous polypropylene. conditions where diffusional limitations of the substrate were not limiting the reaction rate were defined. the specific activity of the lipase was greatly enhanced upon immobilization: 16.5 ... | 1997 | 9237387 |
| induction of biologically active interleukin-8 from lung epithelial cells by burkholderia (pseudomonas) cepacia products. | the frequency of isolation of burkholderia cepacia from the sputum of cystic fibrosis (cf) patients is increasing. using the human a549 lung epithelial cell line, we have investigated the ability of b. cepacia exoproducts to stimulate interleukin-8 (il-8) release. cell-free supernatants from a panel of cf clinical, non-cf clinical, and nonclinical b. cepacia isolates were found to stimulate il-8 release, with levels ranging from 11.8 +/- 2.8 to 80.0 +/- 3.5 ng/ml. a similar pattern was seen at t ... | 1997 | 9009321 |
| first case report of melioidosis in guadeloupe, a french west indies archipelago. | | 1997 | 9243060 |
| [the pathomorphology and pathogenesis of glanders in laboratory animals]. | the pathomorphology and cell-mediated response of the body to burkholderia mallei in laboratory animals, highly sensitive and resistant to these bacteria. in the comparative study of the pathomorphology and pathogenesis of glanders in golden hamsters and white rats quantitative and qualitative differences in the histogenesis of response reaction and the morphology of immunocompetent organs were established. cell-mediated reactions play a greater role in the limitation of the early spread of b. m ... | 1997 | 9245146 |
| polymerase chain reaction for the detection of pseudomonas aeruginosa, stenotrophomonas maltophilia and burkholderia cepacia in sputum of patients with cystic fibrosis. | occurrence of pseudomonas aeruginosa, stenotrophomonas (xanthomonas) maltophilia and burkholderia (pseudomonas) cepacia in sputum of cystic fibrosis (cf) patients was demonstrated with a simple and rapid polymerase chain reaction (pcr) technique. the pcr was performed with a set of three primer pairs based on 16s rrna sequences after sputum preparation with dithiothreitol and naoh lysis. all three pathogens could be individually detected by the use of this technique. to prevent carry-over contam ... | 1996 | 9025076 |
| identifying treatments that halt progression of pulmonary disease in cystic fibrosis. | rapid progress in cystic fibrosis research affords the possibility of halting the progress of the lung disease. we used data from 215 patients who had sputum cultures negative for burkholderia cepacia, at least one outpatient pulmonary function test during 1990, and at least one test a year later to estimate the number of subjects and study duration required to demonstrate that a hypothetical treatment reduces the rate of decline of forced expiratory volume in 1 s (fev1) to zero. mean rate of de ... | 1997 | 9029632 |
| multifactorial pathogenic mechanisms of burkholderia pseudomallei as suggested from comparison with burkholderia cepacia. | with the purpose to elucidate the pathogenesis of disease due to burkholderia pseudomallei some biological and biochemical properties of this species were studied in comparison with b. cepacia, since the difference in the level of virulence between the two species is remarkable despite of their toxonomic closeness. b. pseudomallei was distinct from b. cepacia in the capability to grow under anaerobic conditions, with positive nitrate respiration, excretion of high-molecular polysaccharides into ... | 1996 | 9031412 |
| [pulmonary melioidosis in a german southeast asia tourist]. | a patient who returned from a 3-year stay in thailand and india one year ago, was admitted with fever of 38.5 degrees c and productive cough for the last four weeks. he remembered wounding his foot three years ago in india with contamination by soil. subsequently, recurrent pustulae appeared on his feet. one such pustule was found on admittance. the clinical examination showed low body weight, without further abnormalities. | 1997 | 9265152 |
| comparative in vitro susceptibility of burkholderia pseudomallei to cefpirome, ceftazidime and cefepime. | | 1997 | 9271030 |
| abnormal technetium labelled white cell scan in the colitis of chronic granulomatous disease. | a child with colitis was treated for crohn's disease, diagnosed on history, clinical and colonoscopic findings, radiolabelled white cell bowel scan, and colonic histology. after septicaemia caused by an unusual organism, further investigation lead to a diagnosis of chronic granulomatous disease (cgd). the granulomatous colitis of cgd is clinically, histologically, and on white cell scanning, indistinguishable from that in crohn's disease and should be considered in atypical cases. infection with ... | 1997 | 9279152 |
| the laboratory diagnosis of melioidosis. | the recognition of unusual, but important, pathogens such as burkholderia pseudomallei is essential for the rapid implementation of appropriate antimicrobial therapy--delays can be fatal. melioidosis should be considered as a potential diagnosis for any patient with exposure to areas of endemicity, and thus laboratories should be aware of the differential features of the disease and the causative organism. isolation of b. pseudomallei is readily achieved using standard culture media such as bloo ... | 1996 | 9069100 |
| meliodosis in india. | | 1996 | 9282627 |
| specificity and functional activity of anti-burkholderia pseudomallei polysaccharide antibodies. | the lipopolysaccharide (lps) of burkholderia pseudomallei, the causative agent of melioidosis, consists of two o-antigenic polysaccharides designated o-ps i and o-ps ii. in this study, the o-ps specificity and functional activity of a protective polyclonal antiserum and an immunoglobulin m (igm) monoclonal antibody were determined. the polyclonal antiserum recognized both o-ps i and o-ps ii, while the monoclonal antibody was o-ps ii specific. both mediated phagocytic killing of b. pseudomallei b ... | 1997 | 9284132 |
| [immunogenic potential of glanders and melioidosis agents]. | unlike the glanders agent, the superficial structures of the melioidosis agent were demonstrated to be responsible for marked was immunosuppressive activity. some antigenic fractions suppressing the blast transformation of lymphocytes, reducing the count of t helpers and profoundly potentiating the infection in vivo were isolated from p. pseudomallei cells. the immunogenic and immunosuppressive activities of both agents' superficial structures were studied by high performance chromatography. ant ... | 1997 | 9289277 |
| [effect of tra-system of plasmids rp4 and r68.45 on pseudomonas mallei virulence]. | in pseudomonas mallei, spontaneous mutants (tra- mutants) of the plasmids rp4 and r68.45, losing the ability to transfer at conjugation are formed. the plasmids rp4 and r68.45 with tra(+)-phenotype caused a decrease in p. mallei virulence for laboratory animals. at the same time, tra- mutants of these plasmids do not affect p. mallei virulence. the insertion of dna fragment of about 1900 bp into the plasmid transfer gene regions (tra-2-tra-3) gave rise to rp4 and r68.45 tra mutations detectable ... | 1997 | 9289278 |
| cross-regulation of toluene monooxygenases by the transcriptional activators tbmr and tbut. | the toluene-3-monooxygenase from burkholderia pickettii pko1 and the toluene/benzene-2-monooxygenase from burkholderia (pseudomonas) sp. strain js150 are distinct enzymes which differ not only in catalytic specificity and substrate range but also in the arrangement and sequence of the genes within the operons that encode the enzymes, tbua1ubva2c and tbmabcdef, respectively. in the present study, we examined the transcriptional activation of the ptbua1 and ptbma promoters by their cognate regulat ... | 1997 | 9293027 |
| characterization of the capsular polysaccharide of burkholderia (pseudomonas) pseudomallei 304b. | burkholderia (pseudomonas) pseudomallei is the causative agent of melioidosis, a bacterial infection of considerable morbidity in areas of endemicity of southeast asia and northern australia. clinical isolates of b. pseudomallei have been demonstrated to produce a lipopolysaccharide (lps) containing two separate and chemically distinct antigenic o polysaccharides against which infected patients produced antibodies. a putative capsular polysaccharide (cps) has also been reported and is thought to ... | 1997 | 9294419 |
| detection of burkholderia pseudomallei in blood samples using polymerase chain reaction. | a highly sensitive, specific, rapid and simple method to detect burkholderia pseudomallei in blood samples was developed. two 22-base oligonucleotide primers, based on sequences from a specific dna probe, were used for amplification of bacterial dna by the polymerase chain reaction (pcr). amplification with these primers yielded a 178-base pair product in 100 clinical isolates of b. pseudomallei. as little as 0.5 fg of b. pseudomallei dna was detectable by this method. experiments involving inoc ... | 1997 | 9076711 |
| [the transfer of antibacterial resistance to recipients highly sensitive to glanders]. | in multiple experiments on golden hamsters, highly sensitive to glanders, the method of the transfer of antibacterial resistance was worked out. the most positive effect was obtained after the subcutaneous injection of saline-suspended marrow cells, obtained from white rats, infected with burkholderia mallei c5 in a single inoculation and passaged through noninfected white rats 5 times. out of 60 golden hamsters, treated with such material and subsequently (on day 21) injected with 1.0 x 10(2) b ... | 1997 | 9304339 |
| mobilization, cloning, and sequence determination of a plasmid-encoded polygalacturonase from a phytopathogenic burkholderia (pseudomonas) cepacia. | phytopathogenic strains of burkholderia cepacia (synonym pseudomonas cepacia) produce endopolygalacturonase, whereas strains of clinical and soil origin do not. growth of a phytopathogenic strain (atcc25416) at elevated temperatures resulted in nonpectolytic derivatives that were either cured of a resident plasmid or contained a plasmid of reduced mass. the resident 200-kb plasmid (ppec320) in strain atcc25416 was tagged with tn5-mob. the ppec320::tn5-mob (ppec321) plasmid was mobilized in b. ce ... | 1997 | 9304858 |
| identification by replicon-typing of indigenous plasmids of burkholderia (pseudomonas) solanacearum. | fifteen strains of burkholderia (pseudomonas) solanacearum were isolated, identified from different parts of india and screened for plasmid profile. they harboured 1-3 plasmids, but most of the isolates contained a single plasmid. plasmids were classified on the basis of replicon typing by southern hybridization using incompatible specific rep probes. plasmids of 9 strains hybridized with repp probe, plasmids of 5 strains hybridized with repw probe and one plasmid hybridized with repq probe. fin ... | 1997 | 9315243 |
| [creation of genomic dna libraries for pseudomonas mallei and pseudomonas pseudomallei]. | the genomic libraries of p. mallei and p. pseudomallei species were constructed in escherichia coli. the chromosomal dna of p. pseudomallei c-141 strain has been cloned into the cosmid vector phc79 and the broad host range plasmid vector pes154. the chromosomal dna of p. mallei [symbol: see text]-5 strain has been cloned into the plasmid vector psup202. the recombinant clones of the genomic libraries were screened by the enzyme-linked immunoadsorbent assay (elisa) to detect the production of pse ... | 1997 | 9082182 |
| substituted amines synergistic with tobramycin against burkholderia cepacia in vitro. | antibiotic-resistant burkholderia cepacia is an important pathogen in cystic fibrosis. we have previously shown enhanced tobramycin susceptibility of most strains in the presence of the drug amiloride. others have shown similar findings in the presence of cationic agents with amine groups. to examine the relationship of substituted amine drug structure and synergy potential with tobramycin, the in vitro activity of 45 non-antibiotic substituted amine and related compounds in the presence and abs ... | 1997 | 9084918 |
| arabinose assimilation defines a nonvirulent biotype of burkholderia pseudomallei. | two distinct types of burkholderia pseudomallei, differentiated by the ability to assimilate l-arabinose but with similar morphologies and antigenicities, can be isolated from soil in thailand. approximately 25% of soil isolates from northeast thailand were arabinose assimilators (ara+), but in 1,200 sequentially studied patients, only arabinose "nonassimilators" (ara-) caused melioidosis (p < 0.0001). in a murine model, there was a striking difference in virulence between ara- and ara+ b. pseud ... | 1997 | 9317042 |
| isolation rates of burkholderia pseudomallei among the four regions in thailand. | this study aimed to compare the isolation rates of burkholderia pseudomallei among community-based hospitals located in the central, north, northeast, and south of thailand. a questionnaire inquiring about the number of isolation of b. pseudomallei from various clinical specimens during 1994-95 were mailed to 141 community-based hospitals. of these, 125 hospitals (88.6%) responded to the questionnaire. microbiological laboratory was not available in thirty hospitals. data from 95 remaining hospi ... | 1997 | 9322292 |
| isolation of new 6-methylnicotinic-acid-degrading bacteria, one of which catalyses the regioselective hydroxylation of nicotinic acid at position c2. | 2-hydroxynicotinic acid is an important building block for herbicides and pharmaceuticals. enrichment strategies to increase the chances of finding microorganisms capable of hydroxylating at the c2 position and to avoid the degradation of nicotinic acid via the usual intermediate, 6-hydroxynicotinic acid, were used. three bacterial strains (mena 23/3-3c, mena 25/4-1, and mena 25/ 4-3) were isolated from enrichment cultures with 6-methylnicotinic acid as the sole source of carbon and energy. part ... | 1997 | 9325423 |
| a review of the in vitro activity of meropenem and comparative antimicrobial agents tested against 30,254 aerobic and anaerobic pathogens isolated world wide. | the in vitro activity of meropenem (formerly sm-7738), a new carbapenem, was compared with that of imipenem and five other broad-spectrum antimicrobials (ceftazidime, cefotaxime, piperacillin, piperacillin/tazobactam, and ciprofloxacin) against 30,254 clinically significant pathogens isolated in nine countries worldwide. overall, the carbapenems, meropenem and imipenem, were the most active drugs. meropenem was four- to 64-fold more active than imipenem against gram-negative bacteria, including ... | 1997 | 9327242 |
| in vitro activities of antimicrobial agents, alone and in combinations, against burkholderia cepacia isolated from blood. | burkholderia cepacia is a widespread, environmental gram-negative bacillus that is associated with nosocomial infections. this bacterium is considered to be an important pathogen in immunocompromised patients and is inherently resistant to multiple antimicrobial agents. to compare the activity of different antimicrobial agents and the potential of combinations against invasive strains of b. cepacia, we collected 36 isolates of b. cepacia from blood cultures and checked their susceptibilities to ... | 1997 | 9327247 |
| multiple pathways for toluene degradation in burkholderia sp. strain js150. | burkholderia (pseudomonas) sp. strain js150 uses multiple pathways for the metabolism of catechols that result from degradation of aromatic compounds. this suggests that the strain also uses multiple upstream pathways for the initial hydroxylation of aromatic substrates. two distinct dna fragments that allowed pseudomonas aeruginosa pao1c to grow with benzene as a sole carbon source were cloned from strain js150. one of the recombinant plasmids containing the initial steps for the degradative pa ... | 1997 | 9327568 |
| occurrence of multiple genomovars of burkholderia cepacia in cystic fibrosis patients and proposal of burkholderia multivorans sp. nov. | we performed an integrated genotypic and phenotypic analysis of 128 strains of the genera burkholderia, ralstonia, and pseudomonas in order to study the taxonomic structure of burkholderia cepacia and its relationships with other burkholderia species. our data show that presumed b. cepacia strains isolated from cystic fibrosis patients belong to at least five distinct genomic species, one of which was identified as burkholderia vietnamiensis. this group of five phenotypically similar species is ... | 1997 | 9336927 |
| should patients with cystic fibrosis infected with burkholderia cepacia undergo lung transplantation? | | 1997 | 9337823 |
| a fusion promoter created by a new insertion sequence, is1490, activates transcription of 2,4,5-trichlorophenoxyacetic acid catabolic genes in burkholderia cepacia ac1100. | transposition and transcriptional activation by insertion sequences in burkholderia cepacia ac1100 were investigated. two closely related new elements, is1413 and is1490, were identified and characterized. these elements are not highly related to other insertion sequences identified in ac1100 or other b. cepacia isolates. based on their structures and the sequences of the inverted terminal repeats and the putative transposase protein, the insertion elements (is elements) are similar to ist2 of t ... | 1997 | 9098071 |
| nosocomial acquisition of burkholderia gladioli in patients with cystic fibrosis. | burkholderia gladioli has been reported as colonizing the airways of patients with cystic fibrosis (cf) but has not previously been associated with adverse outcome. we describe six patients with cf in whom the same strain of b. gladioli, on the basis of ribotyping and biochemical characteristics, was grown in their sputum. acquisition of this organism was followed by a fatal outcome in all six patients; one had a rapid decline in respiratory status and another developed fulminant b. gladioli bac ... | 1997 | 9105090 |
| influence of growth rate and nutrient limitation on susceptibility of burkholderia cepacia to ciprofloxacin and tobramycin. | the influence of growth rate and oxygen availability on ciprofloxacin and tobramycin sensitivity and cell surface hydrophobicity in burkholderia cepacia was assessed for cells grown in a chemostat under iron-limitation. whereas susceptibility to both antibiotics decreased with increasing growth rate and oxygen repletion yet increased under oxygen depletion, hydrophobicity decreased with increasing growth rate and oxygen repletion but was relatively unaffected under oxygen depletion. these result ... | 1997 | 9338495 |
| isolation and identification of burkholderia pseudomallei from soil using selective culture techniques and the polymerase chain reaction. | an environmental soil survey to detect burkholderia pseudomallei was performed during the dry and wet seasons in darwin, northern territory, australia. soil was sampled at regular intervals during a 15-month period at different depths from areas which were representative of the local, soil environment. selective culture techniques using ashdown's and galimand and dodin's methods and the polymerase chain reaction (pcr) using specific 16s rrna primers were used to detect and identify the organism ... | 1997 | 9172400 |
| structure of a new 6-deoxy-alpha-d-talan from burkholderia (pseudomonas) plantarii strain dsm 6535, which is different from the o-chain of the lipopolysaccharide. | an o-acetylated homopolysaccharide of 6-deoxy-d-talose (6-deoxy-alpha-d-talan polymer) was isolated from burkholderia (pseudomonas) plantarii strain dsm 6535 by extraction with 2-propanol. the structure (1) of the trisaccharide repeating unit of the polysaccharide was established by studies of the intact and o-deacetylated polysaccharides using methanolysis, methylation analysis, 1h and 13c nmr spectroscopy, including 2d cosy, heteronuclear 13c, 1h cosy, 1d noe, and computer-assisted analysis of ... | 1997 | 9203339 |
| structural elucidation of two polysaccharides present in the lipopolysaccharide of a clinical isolate of burkholderia cepacia. | based on the sugar composition, methylation analyses and smith degradation, supported by nmr spectroscopic analyses and fast-atom-bombardment ms experiments, the lipopolysaccharide produced by a clinical isolate of burkholderia cepacia was shown to contain two distinct polymers, both with linear trisaccharide repeating units; a major, containing d-rhamnose and d-galactose residues (2:1) with the structure -->3)-alpha-d-rhap(1-->3)-alpha-d-rhap(1-->4)-alpha-d-galp(1 --> (major), and a minor repea ... | 1997 | 9208925 |
| a novel means to develop strain-specific dna probes for detecting bacteria in the environment. | a simple means to develop strain-specific dna probes for use in monitoring the movement and survival of bacteria in natural and laboratory ecosystems was developed. the method employed amplification of genomic dna via repetitive sequence-based pcr (rep-pcr) using primers specific for repetitive extragenic palindromic (rep) elements, followed by cloning of the amplified fragments. the cloned fragments were screened to identify those which were strain specific, and these were used as probes for to ... | 1997 | 9212434 |
| biodiversity of a burkholderia cepacia population isolated from the maize rhizosphere at different plant growth stages. | a burkholderia cepacia population naturally occurring in the rhizosphere of zea mays was investigated in order to assess the degree of root association and microbial biodiversity at five stages of plant growth. the bacterial strains isolated on semiselective pcat medium were mostly assigned to the species b. cepacia by an analysis of the restriction patterns produced by amplified dna coding for 16s rrna (16s rdna) (ardra) with the enzyme alui. partial 16s rdna nucleotide sequences of some random ... | 1997 | 9361434 |
| green fluorescent protein as a marker for pseudomonas spp. | the development of sensitive methods for observing individual bacterial cells in a population in experimental models and natural environments, such as in biofilms or on plant roots, is of great importance for studying these systems. we report the construction of plasmids which constitutively express a bright mutant of the green fluorescent protein of the jellyfish aequorea victoria and are stably maintained in pseudomonas spp. we demonstrate the utility of these plasmids to detect individual cel ... | 1997 | 9361441 |
| characterization of the pena and penr genes of burkholderia cepacia 249 which encode the chromosomal class a penicillinase and its lysr-type transcriptional regulator. | burkholderia cepacia is recognized as an important pathogen in the lung infections of patients with cystic fibrosis. an inducible beta-lactamase activity has been associated with increased resistance to beta-lactam antibiotics in clinical isolates of b. cepacia. in this study, we report the revised sequence of the pena gene, which encodes the inducible penicillinase of b. cepacia, and show that it belongs to the molecular class a beta-lactamases and exhibits a high degree of similarity to the ch ... | 1997 | 9371340 |
| infections in patients with cystic fibrosis following lung transplantation. | there is controversy over whether colonization with drug-resistant organisms is a contraindication to lung transplantation. | 1997 | 9377954 |
| identification and characterization of a two-component regulatory system involved in invasion of eukaryotic cells and heavy-metal resistance in burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a disease increasingly recognized as an important cause of morbidity and mortality in many regions of the world. b. pseudomallei is a facultative intracellular pathogen capable of invading eukaryotic cells. we used tn5-ot182 mutagenesis to generate mutants deficient in the ability to invade a human type ii pneumocyte cell line (a549 cells). one of these mutants, aj1d8, exhibited approximately 10% of the ability of the parental stra ... | 1997 | 9393784 |
| genotypic analysis of burkholderia cepacia isolates from 13 french cystic fibrosis centers. | burkholderia cepacia has been involved in outbreaks of pulmonary infection among patients with cystic fibrosis (cf), and the spread of a highly transmissible clone has been reported throughout the united kingdom and canada. these data prompted a dna-based typing study of the strains recovered in french cf centers. ninety-five isolates recovered from 71 patients attending 13 cf centers in 9 regions of france were characterized by randomly amplified polymorphic dna (rapd) analysis and pulsed-field ... | 1997 | 9230381 |
| polymerase chain reaction for the detection of burkholderia pseudomallei. | melioidosis is a potentially lethal infection of humans and animals in southeast asia and northern australia. current methods for detection of the causative organism, burkholderia pseudomallei, lack both speed and sensitivity. we report the development of a highly sensitive polymerase chain reaction-based method that can detect as few as 35 colony-forming units of b. pseudomallei/ml in saline suspensions. this polymerase chain reaction test also detected the presence of b. pseudomallei dna in cu ... | 1997 | 9401803 |
| bacteremia due to burkholderia gladioli: case report. | | 1997 | 9402407 |
| use of bronchoalveolar lavage semiquantitative cultures in cystic fibrosis. | to assess bronchoalveolar lavage (bal) in adult cf patients with respiratory symptoms, we studied bal fluid (balf) culture results from 28 bronchoscopies in 11 patients. patients were asked to provide sputum for culture. all but two patients were receiving antibiotics at the time of bronchoscopy, with 13 bronchoscopies done on patients who had been receiving antibiotics for more than 10 d. gram stain of the balf was positive in 18 cases. in all but one balf, > 10,000 colony-forming units per mil ... | 1997 | 9230762 |
| lipopolysaccharide from burkholderia vietnamiensis strain lmg 6999 contains two polymers identical to those present in the reference strain for burkholderia cepacia serogroup o4. | lipopolysaccharide was isolated from strain lmg 6999 of burkholderia vietnamiensis. degradative and nmr spectroscopic studies established the presence of two polymeric fractions based on the following trisaccharide repeating units: i:-->3)-alpha-d-galp-(1-->3)-beta-d-galp-(1-->3)-beta-d-galpnac- (1-->; ii:-->3)-alpha-d-galpnac-(1-->3)-beta-d-galpnac-(1-->4)- alpha-l-rhap-(1-->. the same polymers have previously been found together in lipopolysaccharide from the reference strain for burkholderia ... | 1997 | 9418254 |
| genetic characterization of insertion sequence isjp4 on plasmid pjp4 from ralstonia eutropha jmp134. | directly adjacent to the (tfdt-) tfdcdef gene cluster for chlorocatechol breakdown on plasmid pjp4 of ralstonia eutropha (formerly alcaligenes eutrophus) jmp134, we identified a 0.9-kb dna element, designated isjp4, with the typical features of a bacterial insertion sequence. isjp4 occurs as a single complete copy on plasmid pjp4. about 9 kb away from this copy, in the tfda-tfds intergenic region, we found a 71-bp duplication of the isjp4 right-hand extremity. in addition, we discovered a comple ... | 1997 | 9427552 |
| ribotyping and dna macrorestriction analysis of isolates of burkholderia pseudomallei from cases of melioidosis in malaysia. | forty-nine isolates of burkholderia pseudomallei from sporadic cases of melioidosis in malaysia over the past 18 years were examined by bamhi ribotyping and pulsed-field gel electrophoresis (pfge) of xbai digests of total deoxyribonucleic acid (dna). twenty-four patients had septicaemic melioidosis with a mortality of 70%; mortality in the non-septicaemic disease was 16%. five ribotype patterns were identified, 2 of which accounted for 90% of all isolates. pfge revealed a number of different str ... | 1997 | 9231217 |
| rhizoremediation of trichloroethylene by a recombinant, root-colonizing pseudomonas fluorescens strain expressing toluene ortho-monooxygenase constitutively. | trichloroethylene (tce) was removed from soils by using a wheat rhizosphere established by coating seeds with a recombinant, tce-degrading pseudomonas fluorescens strain that expresses the toma+ (toluene o-monooxygenase) genes from burkholderia cepacia pr1(23)(tom23c). a transposon integration vector was used to insert toma+ into the chromosome of p. fluorescens 2-79, producing a stable strain that expressed constitutively the monooxygenase at a level of 1.1 nmol/min.mg of protein (initial tce c ... | 1998 | 9435067 |
| burkholderia cepacia bacteremia: a retrospective analysis of 70 episodes. | burkholderia cepacia, a widespread gram-negative environmental bacillus associated with nosocomial infections, is considered to be of relatively low virulence and rarely to cause invasive disease. we retrospectively analyzed the risk factors, clinical manifestations, antimicrobial susceptibilities, and prognostic factors of b. cepacia bacteremia cases. from 1982 through 1995, 70 episodes of bacteremia due to b. cepacia occurred in 52 patients at the national taiwan university hospital. the overa ... | 1997 | 9444917 |
| bacterial associations with mycorrhizal fungi: close and distant friends in the rhizosphere. | bacteria and mycorrhizal fungi in the rhizosphere interact with each other at different levels of cellular integration, ranging from apparently simple association, through surface attachment, to intimate and obligatory symbiosis. this synergism may not only be important in promoting plant growth and health, but may also be significant to rhizosphere ecology. | 1997 | 9447662 |
| toxin production by burkholderia pseudomallei strains and correlation with severity of melioidosis. | an exotoxin lethal to cells in culture (cytolethal toxin, clt) was identified in culture filtrates of burkholderia pseudomallei, the causative organism of melioidosis. clt could pass through a 10-kda cut-off ultrafilter and its properties suggest that it is a peptide. isolates from soil, animals and man showed differential cytolethality in vitro. the isolates were divided into low, medium and high clt producers with soil isolates being low producers and isolates from patients with melioidosis en ... | 1997 | 9236739 |
| speed of detection of burkholderia pseudomallei in blood cultures and its correlation with the clinical outcome. | burkholderia pseudomallei is a major cause of fatalities from nonhospital-acquired, gram-negative bacterial septicemia in northeastern part of thailand. rapid isolation of the bacterium is critical for diagnosis and treatment. bacterial culture is currently the gold standard method for laboratory diagnosis of melioidosis. the present study describes the time to detection of b. pseudomallei in blood cultures using a bact/alert automated blood culture system, and the correlation between the speed ... | 1997 | 9242327 |
| genetic and biochemical characterization of the broad spectrum chlorobenzene dioxygenase from burkholderia sp. strain ps12--dechlorination of 1,2,4,5-tetrachlorobenzene. | the bacterium, burkholderia (previously pseudomonas) sp. strain ps12, reported earlier to degrade 1,2,4-trichlorobenzene is shown here to utilize also 1,2,4,5-tetrachlorobenzene (cl4-benzene) as a growth substrate. to investigate the possibility that this organism attacks cl4-benzene with a chlorobenzene dioxygenase which concomitantly causes dehalogenation, and to analyze the substrate range of the initial enzyme, a 5503-bp dna fragment from ps12, exhibiting high similarity to genes coding for ... | 1997 | 9249026 |
| studies of lipase-catalyzed esterification reactions of some acetylenic fatty acids. | esterification of five positional isomers of acetylenic fatty acids [viz. 9:1(2a), 11:1(10a), 18:1(6a), 18:1(9a) and 22:1(13a)] of different chain lengths with n-butanol in n-hexane in the presence of eight different lipases [lipozyme im 20 (rhizomucor miehei), lipolase 100t (r. miehei), novozyme 435 (candida antarctica), ppl (porcine pancreatic lipase), ccl (c. cylindracea), ps-d (pseudomonas cepacia), lipase a-12 (aspergillus niger) and lipase ay-30 (c. rugosa)] was studied. 2-nonynoic acid wa ... | 1998 | 9470175 |
| construction of a reca mutant of burkholderia (formerly pseudomonas), cepacia. | a reca mutant was constructed of a soil isolate of burkholderia cepacia, strain atcc 17616. prior to mutagenesis, the reca gene was cloned from this strain by its ability to complement the methyl methanesulfonate sensitivity of an escherichia coli reca mutant. sequence analysis of the strain showed high sequence similarity (94% nucleic acid and 99% amino acid identity) with the reca gene previously cloned from a clinical isolate of b. cepacia, strain jn25. the subcloned reca gene from b. cepacia ... | 1998 | 9487711 |
| emerging infections in australia. | over the last 10 years, novel infectious agents including equine morbillivirus, lyssavirus, barmah forest virus, rickettsia honei and two as-yet-unnamed bunyaviruses have been identified as causes of human disease in australia. previously described agents, such as japanese b encephalitis virus, dengue virus, ross river virus, orientia tsutsugamushi, rickettsia australis, burkholderia pseudomallei, mycobacterium ulcerans and trichinella pseudospiralis, have increased their geographical distributi ... | 1997 | 9494666 |
| variation in flagellin genes and proteins of burkholderia cepacia. | the majority of isolates of burkholderia cepacia, an important opportunistic pathogen associated with cystic fibrosis, can be classified into two types on the basis of flagellin protein size. electron microscopic analysis indicates that the flagella of strains with the larger flagellin type (type i) are wider in diameter. flagellin genes representative of both types were cloned and sequenced to design oligonucleotide primers for pcr amplification of the central variable domain of b. cepacia flag ... | 1998 | 9495748 |
| enzyme specificity of 2-nitrotoluene 2,3-dioxygenase from pseudomonas sp. strain js42 is determined by the c-terminal region of the alpha subunit of the oxygenase component. | biotransformations with recombinant escherichia coli expressing the genes encoding 2-nitrotoluene 2,3-dioxygenase (2ntdo) from pseudomonas sp. strain js42 demonstrated that 2ntdo catalyzes the dihydroxylation and/or monohydroxylation of a wide range of aromatic compounds. extremely high nucleotide and deduced amino acid sequence identity exists between the components from 2ntdo and the corresponding components from 2,4-dinitrotoluene dioxygenase (2,4-dntdo) from burkholderia sp. strain dnt (form ... | 1998 | 9495758 |
| bacterial community dynamics during start-up of a trickle-bed bioreactor degrading aromatic compounds. | this study was performed with a laboratory-scale fixed-bed bioreactor degrading a mixture of aromatic compounds (solvesso100). the starter culture for the bioreactor was prepared in a fermentor with a wastewater sample of a care painting facility as the inoculum and solvesso100 as the sole carbon source. the bacterial community dynamics in the fermentor and the bioreactor were examined by a conventional isolation procedure and in situ hybridization with fluorescently labeled rrna-targeted oligon ... | 1998 | 9501433 |