| a pore-forming haemolysin from the hookworm, ancylostoma caninum. | hookworms feed on blood, but the mechanism by which they lyse ingested erythrocytes is unknown. here we show that ancylostoma caninum, the common dog hookworm, expresses a detergent soluble, haemolytic factor. activity was identified in both adult and larval stages, was heat-stable and unaffected by the addition of protease inhibitors, metal ions, chelators and reducing agents. trypsin ablated lysis indicating that the haemolysin is a protein. a closely migrating doublet of hookworm proteins wit ... | 2004 | 15313129 |
| ac-saa-1, an immunodominant 16 kda surface-associated antigen of infective larvae and adults of ancylostoma caninum. | a cdna encoding a surface-associated antigen was cloned from an ancylostoma caninum infective larvae (l(3)) cdna library by immunoscreening with pooled human immune sera. the sera were obtained from individuals living in an ancylostoma duodenale hookworm-endemic region of china, who had light intensity infections and high antibody titers against a. caninum l(3). ancylostoma caninum surface-associated antigen-1 is encoded by an 843 bp mrna with a predicted open reading frame of 162 amino acids. r ... | 2004 | 15313130 |
| application of a species-specific pcr-rflp to identify ancylostoma eggs directly from canine faeces. | apart from their veterinary importance, the hookworms ancylostoma caninum, ancylostoma braziliense and ancylostoma caninum are also capable of causing zoonotic disease in humans. a highly sensitive and species-specific pcr-rflp technique was utilised to detect and differentiate the various canine ancylostoma spp directly from eggs in faeces. this technique was utilised to screen 101 canine faecal samples from parasite endemic tea growing communities in assam, india, as part as an ongoing epidemi ... | 2004 | 15325050 |
| amphidial neurons adl and ash initiate sodium dodecyl sulphate avoidance responses in the infective larva of the dog hookworm anclyostoma caninum. | ablations of specific amphidial neuron pairs with a laser microbeam were conducted to understand better the neurological basis of the behaviours of larval parasitic nematodes. to date, the functions of the amphidial neurons of caenorhabditis elegans and their counterparts in parasitic nematodes have been found to be remarkably conserved allowing the possibility to predict the relationships between neurons and their functions. therefore, we anticipated that ablation of neuron pairs ash and ask wo ... | 2004 | 15542093 |
| identification of specific male and female genes in adult ancylostoma caninum. | the hookworm ancylostoma canium represents a serious health problem, not only for animals but also for humans. these blood-feeding parasites produce various proteolytic enzymes in order to digest the host hemoglobin. the female worm ingests more blood than does the male. it is not known whether this difference is accompanied by expression of sex-specific proteinases. the identification of new genes related either to the developmental process of maturation of each sex or to the proteinases secret ... | 2004 | 15604493 |
| infective larvae of the human hookworms necator americanus and ancylostoma duodenale differ in their orientation behaviour when crawling on surfaces. | the infective third stage larvae of hookworms infect their hosts by active skin invasion, and they find and recognize their hosts by the behavioural phases of activation, directed crawling, and penetration. here we analyse the orientation of the infective larvae of the human hookworms necator americanus and ancylostoma duodenale when crawling on surfaces. their behaviour differed from that of the larvae of the dog hookworm ancylostoma caninum, but the two species also differed from each other. n ... | 2004 | 15614586 |
| ecological analysis and description of the intestinal helminths present in dogs in mexico city. | the objective of the present study was to determine the prevalence of parasitoses in stray dogs from mexico city, as well as to describe the structure of the intestinal helminth communities present in said hosts. one hundred and twenty dog intestines were collected and dissected to evaluate their parasitic load; finding parasites in 102 dogs (85%). the cestodes collected were: dipylidium caninum in 72 dogs (60%), taenia hydatigena in three (2.5%), taenia pisiformis in two (1.6%), echinococcus gr ... | 2004 | 15631907 |
| molecular analysis of astacin-like metalloproteases of ostertagia ostertagi. | in this study, we describe the molecular analysis of zinc-metalloproteases from the abomasal nematode ostertagia ostertagi which were exclusively recognized by local antibodies of immune cattle. full-length or partial coding sequences of 4 different zinc-metalloprotease cdnas of ostertagia (met-1, -2, -3 and -4) were amplified using gene-specific primers using the 3'- and 5'-rapid amplification of cdna ends (race) technique. sequence analysis identified the cdnas as encoding zinc-metalloprotease ... | 2005 | 15700760 |
| cloning and characterisation of an aspartyl protease inhibitor (api-1) from ancylostoma hookworms. | hookworm infection persists as a public health problem in developing nations. vaccine-based strategies offer the best chance of long-term control. aspartyl protease inhibitors from parasitic nematodes are highly immunogenic, and have been suggested as potential vaccine antigens. an aspartyl protease inhibitor, api-1, was cloned and characterised from the hookworms ancylostoma caninum and ancylostoma ceylanicum. using sequence from the hookworm expressed sequence tag project, specific primers wer ... | 2005 | 15722082 |
| tribendimidine: a promising, safe and broad-spectrum anthelmintic agent from china. | we review, for the first time, a 20-year chinese story of research and development pertaining to tribendimidine, a promising anthelmintic agent that is safe and exhibits a broad spectrum of activity. tribendimidine was first synthesized at the national institute of parasitic diseases in shanghai in the mid 1980s. in laboratory studies, tribendimidine showed high efficacy against nippostrongylus braziliensis in rats, necator americanus in hamsters, ancylostoma caninum and toxocara canis in dogs, ... | 2005 | 15777691 |
| liver phosphatases in mice treated with lead during murine ancylostomiasis. | both infective larvae and pollutants disturb the homeostasis of female swiss albino mice, causing ancylostomiasis and severe immunophysiological changes. in our experimental design, mice in group a were fed 0.01 mg of lead nitrate before infection with 500 larvae of ancylostoma caninum, mice in group b were infected with larvae without previous lead treatment, and group c received lead nitrate treatment alone. control animals in group d were neither treated with lead nitrate nor infected with la ... | 2005 | 15814320 |
| inactivation of cd11b in a mouse transgenic model protects against sepsis-induced lung pmn infiltration and vascular injury. | to inactivate chronically the beta2-integrin cd11b (mac-1), we made a transgenic model in mice in which we expressed the cd11b antagonist polypeptide neutrophil inhibitory factor (nif). using these mice, we determined the in vivo effects of cd11b inactivation on polymorphonuclear leukocyte (pmn) function and acute lung injury (ali) induced by escherichia coli septicemia. in wild-type pmns, cd11b expression was induced within 1 h after e. coli challenge, whereas this response was significantly re ... | 2005 | 15831844 |
| investigating hookworm genomes by comparative analysis of two ancylostoma species. | hookworms, infecting over one billion people, are the mostly closely related major human parasites to the model nematode caenorhabditis elegans. applying genomics techniques to these species, we analyzed 3,840 and 3,149 genes from ancylostoma caninum and a. ceylanicum. | 2005 | 15854223 |
| new oral linguiform projections and their associated neurons in the third-stage infective larva of the parasitic nematode oesophagostomum dentatum. | the infective larvae (l3i) of the nematode parasite of swine, oesophagostomum dentatum, are passively ingested by their hosts. the l3i exhibit certain behaviors that are probably selected to increase the likelihood of ingestion, by strategic positioning in the environment. the larvae show positive geotactic behavior and respond to temperature variations in their environment, as shown by their behavior on a thermal gradient. to investigate neuronal control of this behavior, we initiated a study o ... | 2005 | 15856873 |
| rag1 core and v(d)j recombination signal sequences were derived from transib transposons. | the v(d)j recombination reaction in jawed vertebrates is catalyzed by the rag1 and rag2 proteins, which are believed to have emerged approximately 500 million years ago from transposon-encoded proteins. yet no transposase sequence similar to rag1 or rag2 has been found. here we show that the approximately 600-amino acid "core" region of rag1 required for its catalytic activity is significantly similar to the transposase encoded by dna transposons that belong to the transib superfamily. this supe ... | 2005 | 15898832 |
| cloning and characterization of a novel immunogenic protein 3 (nip3) from brugia malayi by immuno screening of a phage-display cdna expression library. | iterative screening of a phage display cdna expression library of the third-stage larvae (l3) of brugia malayi with sera from putatively immune individuals (endemic normal, en) identified a novel clone with insert showing significant homology to onchocerca volvulus novel immunogenic protein-3 (ov-nip3) gene and caenorhabditis elegans nip3-like protein and hence the gene was designated brugia malayi nip3-like protein (bmnip3). est database analysis showed that ests from several gastrointestinal n ... | 2005 | 15952042 |
| gene expression profiles associated with the transition to parasitism in ancylostoma caninum larvae. | ancylostoma caninum is a common canine parasite responsible for anemia and death in infected dogs. gene expression profiling was used to investigate molecular differences between two different forms of the third larval stage (l3s): infective free-living larvae and in vitro serum-stimulated larvae that mimic the initial stages of parasitism of a host. we developed an a. caninum cdna microarray consisting of 4191 est clones, and used it to identify a set of 113 genes that are differentially regula ... | 2005 | 15979737 |
| evaluation of endogenous reference genes for real-time pcr quantification of gene expression in ancylostoma caninum. | | 2005 | 15993958 |
| isolation of anthelmintic resistant ancylostoma caninum. | | 1987 | 16031355 |
| identification of a daf-7 ortholog from the hookworm ancylostoma caninum. | infective hookworm l3 encounter a host specific signal during invasion that re-activates suspended developmental pathways. response to this cue is critical for the successful infection and completion of the life cycle in the host. in the free-living nematode caenorhabditis elegans, recovery from the developmentally arrested dauer stage in response to environmental cues is analogous to the resumption of development in invading hookworm l3. transforming growth factor beta (tgf-beta) and insulin-li ... | 2005 | 16135366 |
| cloning and characterisation of genes encoding two transforming growth factor-beta-like ligands from the hookworm, ancylostoma caninum. | to elucidate the role of transforming growth factor beta (tgf-beta) signalling in the arrest/reactivation pathway of the ancylostoma caninum hookworm, two parasite-encoded tgf-beta-like ligands were cloned and characterised. ac-dbl-1 showed 60% amino acid identity to the caenorhabditis elegansdbl-1 gene, which regulates growth while ac-daf-7 showed 46% amino acid identity to ce-daf-7 which regulates arrested development. exon/intron organisation of the genes for ac-dbl-1 and ac-daf-7 were differ ... | 2005 | 16140304 |
| vaccination with irradiated ancylostoma caninum third stage larvae induces a th2 protective response in dogs. | while x-irradiated live parasites are not an acceptable proposition for human vaccination, they offer a ready experimental system to explore mechanisms by which immunity against hookworm infection may be induced in humans. as such, we sought to further elucidate the details of this highly protective immune response induced by the irradiated vaccine in canids, with special emphasis on the cellular aspects of the response. vaccination with irradiated l3 induced high production of antibodies and st ... | 2006 | 16140437 |
| biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm ancylostoma caninum. | we report the cloning and expression of ac-gst-1, a novel glutathione s-transferase from the adult hookworm ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. the predicted ac-gst-1 open reading frame contains 207 amino acids (mass, 24 kda) and exhibited up to 65% amino acid identity with other nematode gsts. mrna encoding ac-gst-1 was detected in adults, eggs, and larval stages, but the protein was detected only in adult hookworm somatic extracts and e ... | 2005 | 16177370 |
| vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. | hookworms infect 730 million people in developing countries where they are a leading cause of intestinal blood loss and iron-deficiency anemia. at the site of attachment to the host, adult hookworms ingest blood and lyse the erythrocytes to release hemoglobin. the parasites subsequently digest hemoglobin in their intestines using a cascade of proteolysis that begins with the ancylostoma caninum aspartic protease 1, apr-1. | 2005 | 16231975 |
| enhancement of protein secretion in pichia pastoris by overexpression of protein disulfide isomerase. | a potential vaccine candidate, necator americanus secretory protein (na-asp1), against hookworm infections, has been expressed in pichia pastoris. na-asp1, a 45 kda protein containing 20 cysteines, was directed outside the cell by fusing the protein to the preprosequence of the alpha-mating factor of saccharomyces cerevisiae. most of the protein produced by single copy clones was secreted outside the cell. however, increasing gene copy number of na-asp1 protein in p. pastoris saturated secretory ... | 2006 | 16255058 |
| [prevalence of intestinal helminths in dogs from quindío province]. | intestinal helminths are pathogens for domestic animals and provide a source of potential infection for humans. | 2005 | 16276682 |
| molecular cloning and functional characterization of an aspartic protease from the hard tick haemaphysalis longicornis. | haemaphysalis longicornis cdna encoding an aspartic protease (longepsin) was identified from a midgut cdna library. the longepsin cdna contains 1176bp that code for 392 amino acid residues with a predictable molecular weight of 39.3kda. the cdna has a signal peptide sequence associated with the n-terminal domains and domain structure analysis revealed that the deduced protein has two aspartic acid residues that are characteristic of a single active site for aspartic proteases. this novel longeps ... | 2006 | 16360947 |
| an epidemiological study of gastrointestinal parasites of dogs from southern greater buenos aires (argentina): age, gender, breed, mixed infections, and seasonal and spatial patterns. | a total of 2193 fecal samples from owned dogs were collected during the 2003-2004 period in southern greater buenos aires, and were evaluated for the presence of intestinal parasites by a flotation-centrifugation method. the overall prevalence was 52.4%, and the 11 species found were: ancylostoma caninum (13%), isospora ohioensis complex (12%), toxocara canis (11%), trichuris vulpis (10%), sarcocystis sp. (10%), giardia duodenalis (9%), isospora canis (3%), hammondia-neospora complex (3%), dipil ... | 2006 | 16364551 |
| ancylostoma caninum mtp-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration. | infective larvae (l3) of nematodes secrete macromolecules that are critical to infection and establishment of the parasite in the host. the dog hookworm ancylostoma caninum secretes an astacin-like metalloprotease, ac-mtp-1, upon activation in vitro with host serum. recombinant ac-mtp-1 was expressed in the baculovirus/insect cell system as a secreted protein and was purified from culture medium by two separate methods, cation-exchange fast-performance liquid chromatography and gelatin-affinity ... | 2006 | 16428741 |
| the dingo non-long terminal repeat retrotransposons from the genome of the hookworm, ancylostoma caninum. | members of the retrotransposable element (rte) clade of non-long terminal repeat (ltr) retrotransposon are widely distributed among eukaryote taxa, with representatives known from caenorhabditis elegans, mammals, mosquitoes, schistosomes, and other taxa. an rte retrotransposon has not, however, been characterized in detail from a parasitic nematode. here, we characterize two discrete copies of an rte-like non-ltr retrotransposon from the genome of the dog hookworm, ancylostoma caninum. the eleme ... | 2006 | 16445914 |
| intestinal macro- and microparasites of wolves (canis lupus l.) from north-eastern poland recovered by coprological study. | wolf scats collected during ecological studies in mazury lake district in ne poland were analysed for intestinal micro- and macroparasites. five nematode species were identified: ancylostoma caninum (ercolani, 1859), uncinaria stenocephala (railliet, 1884), trichuris vulpis (froelich, 1789), toxocara canis (werner, 1782) and toxascaris leonina (von linstow, 1902). among cestode species there were identified infections with dipylidium caninum (linnaeus, 1785). the overall helminth prevalence was ... | 2005 | 16457480 |
| prevalence of zoonotic agents in dogs visiting hospitalized people in ontario: implications for infection control. | visitation of hospitalized people by dogs is becoming commonplace, but little is known about the potential health risks of introducing dogs to healthcare settings. this cross-sectional study evaluated the prevalence of zoonotic agents in a group of 102 visitation dogs from a variety of sources across ontario. between may and july 2004, owners were interviewed by a standardized questionnaire while dogs underwent a standardized physical examination. one specimen of faeces, hair-coat brushings and ... | 2006 | 16466831 |
| a survey of the intestinal transcriptomes of the hookworms, necator americanus and ancylostoma caninum, using tissues isolated by laser microdissection microscopy. | the gastrointestinal tracts of multi-cellular blood-feeding parasites are targets for vaccines and drugs. recently, recombinant vaccines that interrupt the digestion of blood in the hookworm gut have shown efficacy, so we explored the intestinal transcriptomes of the human and canine hookworms, necator americanus and ancylostoma caninum, respectively. we used laser microdissection microscopy to dissect gut tissue from the parasites, extracted the rna and generated cdna libraries. a total of 480 ... | 2006 | 16545815 |
| the ever-expanding neuropeptide gene families in the nematode caenorhabditis elegans. | neuropeptides act as chemical signals in the nervous system to modulate behaviour. with the ongoing est projects and dna sequence determination of different genomes, the identification of neuropeptide genes has been made easier. despite the relatively 'simple' repertoire of behaviours in the nematode caenorhabditis elegans, this worm contains a surprisingly large and diverse set of neuropeptide genes. at least 109 genes encoding over 250 potential neuropeptides have been identified in c. elegans ... | 2005 | 16569285 |
| helminths of red foxes (vulpes vulpes) in denmark. | an epidemiological study of helminths in 1040 red foxes collected from various localities in denmark during 1997-2002, revealed 21 helminth species at autopsy, including nine nematode species: capillaria plica (prevalence 80.5%), capillaria aerophila (74.1%), crenosoma vulpis (17.4%), angiostrongylus vasorum (48.6% from northern zealand (endemic area)), toxocara canis (59.4%), toxascaris leonina (0.6%), uncinaria stenocephala (68.6%), ancylostoma caninum (0.6%), and trichuris vulpis (0.5%); seve ... | 2006 | 16580775 |
| local activation of the tissue factor-factor viia pathway in patients with pneumonia and the effect of inhibition of this pathway in murine pneumococcal pneumonia. | the tissue factor (tf)-factor viia (fviia) complex not only is essential for activation of blood coagulation but also affect the inflammatory response during sepsis. the objective of this study was to determine the role of tf-fviia in pneumonia caused by streptococcus pneumoniae, the most important causative organism in community-acquired pneumonia and a major cause of sepsis. | 2006 | 16625114 |
| prevalence of helminthes in adult dogs in heilongjiang province, the people's republic of china. | the prevalence of helminthes in adult dogs was investigated in heilongjiang province, the people's republic of china, between 1996 and 2004. a total of 178 adult farm dogs from representative geographical locations in heilongjiang province were killed and examined for the presence of helminthes using a helminthological approach. the worms were examined, counted, and identified to species according to existing keys and descriptions. a total of 17 species of helminthes were found to infect dogs, a ... | 2006 | 16715234 |
| endotoxaemia induces resistance to activated protein c in healthy humans. | systemic inflammation activates the tissue factor/factor viia complex (tf/fviia), leading to a procoagulant state, which may be enhanced by impairment of physiological anticoagulant pathways, such as the protein c system. besides impaired protein c activation, resistance to activated protein c (apc) may occur. we studied the effect of endotoxemia on apc resistance, analysed its determinants and evaluated the effect of tf/fviia inhibition on endotoxin-induced apc resistance. sixteen healthy male ... | 2006 | 16846480 |
| [construction and expression of protein self-splicing prokaryotic expression vector ptwin1- acapc2]. | to express recombinant ancylostoma caninum anticoagulant peptide-c2 (acapc2), a whole cdna fragment encoding acapc2 was achieved by ligation- pcr and inserted into prokaryotic expression vector ptwin1 for constructing the specific self-splicing prokaryotic expression vector, ptwin1-acapc2; positive recombinants were transformed into e. coli er2566 for expression research. the recombinant protein, acapc2-intein2-cbd, was soluble and expressed in e. coli er2566 (about 30.1% fusion protein in total ... | 2006 | 16856404 |
| gastrointestinal helminths of free-ranging florida panthers (puma concolor coryi) and the efficacy of the current anthelmintic treatment protocol. | thirty-five florida panthers (puma concolor coryi [bangs, 1899]) collected from six counties in southern florida between 1978 and 2003 were examined at necropsy for gastrointestinal helminths. the panthers were placed into two groups: 1) treated with anthelmintics (n = 17), and 2) untreated (n = 18). nine species of helminths (one trematode, six nematodes, and two cestodes) were identified in the untreated panthers. the most prevalent helminths were alaria marcianae (larue, 1917) (100%), spirome ... | 2006 | 16870865 |
| geographical information systems and canine faecal contamination: the experience in the city of naples (southern italy). | geographical information systems (gis) can be used to make transect sampling when non-moving objects are to be counted, involving choosing a line or series of lines along which the counts are to take place. this approach has been used by us in order to study canine faecal contamination in the city of naples (southern italy), and to evaluate the consequent presence of canine parasitic elements. a gis was constructed utilizing the geo-referenced digital photographs and the cadastral maps of naples ... | 2006 | 16881413 |
| [soil contamination with toxocara spp. eggs in the elblag area]. | the distribution of toxocara spp. eggs in elbl4g was studied. out of 72 soil samples collected in public places of the city 13.9% were positive and the mean egg density was 3.75/100g soil. the city backyards were much more contaminated with toxocara spp. eggs (18.0%) than the playgrounds (4.5%). in sandpits examined the eggs were not found. almost 80% of toxocara spp. eggs recovered were infective. t. cati eggs were more frequent than t. canis eggs. additionally in examined samples two eggs of a ... | 2001 | 16888965 |
| a study on intestinal helminthes of dogs, foxes and jackals in the western part of iran. | human infection especially with helminth parasites is an emerging health issue, as the human environment is increasingly shared with infected animals, either pets or wild life. in this survey, the intestinal content of 83 stray dogs, 22 red foxes and 10 golden jackals collected from the west azarbaijan, kordestan and kermanshah provinces in the west of iran, were studied for the presence of helminth parasites. the percentage of different species recovered from these animals is listed as follows: ... | 2006 | 16899340 |
| progressive tissue injury in burns is reduced by rnapc2. | burn wounds are characterised by central necrosis surrounded by an area of stasis with compromised perfusion. secondary aggravation of the burn wound due to ischaemia in the zone of stasis can also result in necrosis. this study aims to improve circulation in the zone of stasis by reducing microthrombus formation and thereby to reduce secondary aggravation. | 2006 | 16905262 |
| [the occurrence of intestinal nematodes in red foxes in the western pomerania]. | in the period from november 2004 to march 2005 the prevalence of gastro-intestinal nematodes of 165 foxes (vulpes vulpes l.) from the pomerania region was examined. these parasites were found in 66.1% of examined foxes. toxocara canis was the most frequent parasite and was observed in 33.9% of examined animals. toxascaris leonina was found in 1.2%, uncinaria stenocephala in 25.5%, ancylostoma caninum in 6.7 and trichuris vulpis in 10.3% of the examined foxes. | 2005 | 16913531 |
| canine faecal contamination and parasitic risk in the city of naples (southern italy). | dogs are associated with more than 60 zoonotic diseases among which, parasitosis and, in particular, helminthosis, can pose serious public-health concerns worldwide. many canine gastrointestinal parasites eliminate their dispersion elements (eggs, larvae, oocysts) by the faecal route. the quantity of canine faeces deposited on public and private property in cities worldwide is both a perennial nuisance and an important health issue. public sites such as playgrounds, parks, gardens, public square ... | 2006 | 16995934 |
| [parasitic dead-end: update]. | parasitic dead-ends occur when a parasite is unable to establish a permanent interaction in an unnatural host. although the likelihood of successful reproduction by the pathogenic agent is nul, parasitic dead-end heralds capture of new parasites and therefore expansion of the host range. angiostrongyliasis due to a. cantonensis or a. costaricensis, anisakiasis, ancylostoma caninum infection, gnathostomiasis and sparganosis are undoubtedly emerging zoonoses of particular medical interest. prevent ... | 2006 | 16999036 |
| confirmation of sex-specific transcripts from ancylostoma caninum adult worms by semi-quantitative rt-pcr. | in a previous work ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific rt-pcr comparing male and female cdna. in this work more fragments had been identified and a semi-quantitative rt-pcr carried out in order to confirm the sex-specific character of the transcripts obtained. four fragments were confirmed as sex-specific or as being expressed more in one sex than the other. | 2007 | 17005221 |
| high-level pyrantel resistance in the hookworm ancylostoma caninum. | while anthelmintic resistance is now a widely recognized issue in the livestock industries, its existence within companion animal medicine has been rarely established conclusively. we undertook a placebo-controlled in vivo trial to measure the efficacy of pyrantel embonate against pooled isolates of the hookworm ancylostoma caninum from brisbane, australia. a statistically significant fall in adult worm burden was observed among dogs in the pyrantel treatment group compared to the control dogs ( ... | 2007 | 17011128 |
| hookworm-provoked ige-mediated pathology: capricious damage or remarkable strategy? | the existence of the canine hookworm ancylostoma caninum in the human gut seems to be curtailed by an effective ige-mediated inflammatory response against the helminth's feeding secretions, which, by damaging the host, compromises the parasite's feeding opportunities. in contrast, immunity does not develop against adapted, anthropophilic hookworm species, and it is suggested this relates to modulation of the inflammatory response by hookworm secretions. here, john croese discusses this hypothesi ... | 1998 | 17040701 |
| protein profile analysis from advanced third-stage larvae (advl3) and adult worms of gnathostoma binucleatum (nematoda: spirurida). | proteins from crude extracts of advanced third-stage larvae and adult gnathostoma binucleatum nematode worms showed protein profiles in sds-page analysis similar to echinococcus granulosus, trichinella spiralis, dipylidium caninum, ancylostoma caninum, ascaris lumbricoides and toxocara canis. the immunoblot analysis of the human serum infected or suspected to be infected with g. binucleatum using the total larvae extract recognized the 40, 60, 80 and 115 kda proteins and using the total adult wo ... | 2007 | 17096141 |
| saposin-like proteins from the intestine of the blood-feeding hookworm, ancylostoma caninum. | hookworms feed on blood, utilizing haemoglobin for nutrition, growth and reproduction. the haemoglobin digestion cascade has been partially elucidated, but the process immediately preceding this event, haemolysis, has received considerably less attention. we have cloned and expressed ancylostoma caninum mrnas encoding 2 proteins belonging to the saposin-like protein (saplip) family, termed ac-slp-1 and ac-slp-2. the open reading frames of slp-1 and slp-2 were used to identify expressed sequence ... | 2007 | 17109779 |
| [prevalence of helminths in dogs in the region of van and their potential public health significance]. | this study was performed in order to determine the prevalence of helminth infections among dogs and to emphasize the importance of this concept for public health. all selected dogs were from the city of van and a total of 115 fecal samples were examined. of these, 61 were from stray dogs, 26 were breeders and the final 28 dogs were dogs belonging to institutions. all samples were examined in the laboratory of the parasitology laboratory of the faculty of veterinary medicine, yuzuncu yil universi ... | 2006 | 17124658 |
| development and characterization of microsatellite markers for the canine hookworm, ancylostoma caninum. | microsatellites are repetitive genomic elements that show high levels of variation and therefore provide excellent tools to study the genetics of eukaryotic organisms. hookworms are extremely common and important nematode parasites of humans and animals, causing potentially serious disease morbidity. control of hookworms in dogs is achieved by frequent treatment with anthelmintics, and in humans, anthelmintics are frequently administered in a mass-treatment community-wide approach. understanding ... | 2007 | 17136387 |
| intermolecular interactions and characterization of the novel factor xa exosite involved in macromolecular recognition and inhibition: crystal structure of human gla-domainless factor xa complexed with the anticoagulant protein napc2 from the hematophagous nematode ancylostoma caninum. | napc2, an anticoagulant protein from the hematophagous nematode ancylostoma caninum evaluated in phase-ii/iia clinical trials, inhibits the extrinsic blood coagulation pathway by a two step mechanism, initially interacting with the hitherto uncharacterized factor xa exosite involved in macromolecular recognition and subsequently inhibiting factor viia (k(i)=8.4 pm) of the factor viia/tissue factor complex. napc2 is highly flexible, becoming partially ordered and undergoing significant structural ... | 2007 | 17173931 |
| field evaluation of the efficacy and the safety of a combination of oxantel/pyrantel/praziquantel in the treatment of naturally acquired gastrointestinal nematode and/or cestode infestations in dogs in europe. | in five multicentre field trials, the efficacy and safety of a combination of oxantel/pyrantel/praziquantel (dolpac), vetoquinol sa) in the treatment of naturally acquired gastrointestinal nematode and/or cestode infestation in dogs was evaluated in northern and southern europe. forty-eight investigators from france, belgium, germany, italy and spain enrolled 329 dogs to be treated with the tested combination; 235 of these dogs complied with the inclusion criteria of the protocol and had a teste ... | 2007 | 17184919 |
| reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant ac-16. | hookworm infection is one of most important parasitic infection of humans, occurring in 740 million people. here we report the protective vaccination of dogs with ac-16, an immunodominant surface antigen from the hookworm ancylostoma caninum. we show that immunization with ac-16 formulated with as03 elicited specific humoral and cellular immune responses and provided partial protection against hookworm infection and morbidity as evidenced by a significant reduction of hookworm egg counts (64% re ... | 2007 | 17267592 |
| molecular cloning and characterization of ac-mtp-2, an astacin-like metalloprotease released by adult ancylostoma caninum. | ac-mtp-2 is an astacin-like metalloprotease secreted by adult ancylostoma caninum hookworms. ac-mtp-2 cdna was cloned by immunoscreening a cdna library with antisera prepared against adult a. caninum excretory/secretory (es) products. the full-length ac-mtp-2 contains 850 bp cdna encoding a 233 amino acid open reading frame (orf) with 32% amino acid identity to ce-nsp-4, a pharyngeal cell-derived secreted metalloprotease of the nematode caenorhabditis elegans. the predicted orf contained a conse ... | 2007 | 17280728 |
| new anticoagulants for the prevention and treatment of venous thromboembolism. | anticoagulant therapy is effective at preventing the development of venous thromboembolism in high-risk patients, and reduces morbidity and mortality in individuals with established thromboembolic disease. vitamin k antagonists and heparins are currently the most commonly used anticoagulant drugs, but they have practical limitations. therefore, new antithrombotic agents with predictable dose-responses (thereby decreasing the need for monitoring without compromising efficacy or safety), ideally a ... | 2005 | 17319097 |
| protective immunity elicited by ultraviolet-irradiated third-stage infective hookworm (necator americanus and ancylostoma caninum) larvae in mice and hamsters. | the protective immunity elicited by ultraviolet-irradiated third-stage infective larvae of necator americanus (uv-nal3) and ancylostoma caninum (uv-acl3) was evaluated in laboratory mice (a non-permissive model) and hamsters (a permissive model). after optimizing the time of exposure to uv-irradiation, both oral and subcutaneous vaccination routes with uv-acl3 in mice were explored. oral vaccination was more effective at reducing the number of challenge acl3 entering the lungs, whereas subcutane ... | 2006 | 17333729 |
| arthrostoma miyazakiense (nematoda: ancylostomatidae) infection in raccoon dogs of korea and experimental transmission to dogs. | arthrostoma miyazakiense (nematoda: ancylostomatidae) is a hookworm species reported from the small intestines of raccoon dogs (nyctereutes procyonoides) in japan. five korean raccoon dogs (n. procyonoides koreensis) caught from 2002 to 2005 in jeollanam-do (province), a southeastern area of south korea, contained helminth eggs belonging to 4 genera (roundworm, hookworm, whipworm, and capillaria spp.) and cysts of giardia sp. in their feces. necropsy findings of 1 raccoon dog revealed a large nu ... | 2007 | 17570975 |
| echinococcosis (zoonotic hydatidosis) in street dogs in urban and rural areas, dakahlia governorate, egypt. | a total of one hundred and ninety street dogs were captured from urban area, mansoura district and three hundreds and fifty from rural area, met el-korama and adjacent villages (manshet el-badawy, talka center). the overall prevalence of echinoccocus granulosus was 5%, with a worm burden ranging from 4 to 1010 (mean = 421). the prevalence was 6% in rural locality and 3.2% in urban locality. e. granulosus in dogs was significantly higher in rural areas but, without significant difference in puppi ... | 2007 | 17580584 |
| active and exo-site inhibition of human factor xa: structure of des-gla factor xa inhibited by nap5, a potent nematode anticoagulant protein from ancylostoma caninum. | hookworms are hematophagous nematodes capable of growth, development and subsistence in living host systems such as humans and other mammals. approximately one billion, or one in six, people worldwide are infected by hookworms causing gastrointestinal blood loss and iron deficiency anemia. the hematophagous hookworm ancylostoma caninum produces a family of small, disulfide-linked protein anticoagulants (75-84 amino acid residues). one of these nematode anticoagulant proteins, nap5, inhibits the ... | 2007 | 17588602 |
| recombinant nematode anticoagulant protein c2 in patients with non-st-segment elevation acute coronary syndrome: the anthem-timi-32 trial. | we sought to evaluate the safety and efficacy of recombinant nematode anticoagulant protein c2 (rnapc2) in patients with non-st-segment elevation acute coronary syndrome (nste-acs). | 2007 | 17599602 |
| gastrointestinal nematodes in dogs from debre zeit, ethiopia. | the study was conducted during the period between january 2005 and june 2006 to determine the frequency of gastrointestinal (gi) nematode infections of dogs in and around debre zeit, using qualitative and quantitative coprological (n = 100) and postmortem examinations (n = 20). by coproscopy 51% dogs were positive for different types of nematodal eggs, out of which 23.5% were with mixed infections. on necropsy 95% animals were found positive for adult parasites, of which 31.6% were showing more ... | 2007 | 17614203 |
| the evaluation of recombinant hookworm antigens as vaccines in hamsters (mesocricetus auratus) challenged with human hookworm, necator americanus. | we have previously reported the successful adaptation of human hookworm necator americanus in the golden hamster, mesocricetus auratus. this animal model was used to test a battery of hookworm (n. americanus and ancylostoma caninum) recombinant antigens as potential vaccine antigens. hamsters immunized a leading vaccine candidate n. americanus-ancylostoma secreted protein 2 (na-asp-2) and challenged with n. americanus infective larvae (l3), resulted in 30-46.2% worm reduction over the course of ... | 2008 | 17645877 |
| pyrantel in small animal medicine: 30 years on. | pyrantel, a tetrahydropyrimidine nicotinic agonist anthelmintic, has been used in companion animal medicine since the 1970s to control two important nematode groups, the hookworms and the roundworms. given the zoonotic potential of these parasites, pyrantel has served a dual role in helping to protect the health of both companion animals and the public for more than 30 years. this review describes the history and mechanism of action of this drug, and collates evidence that resistance to pyrantel ... | 2008 | 17720556 |
| evaluation of the transcription level of the protein disulfide isomerase in different stages from ancylostoma caninum with a real-time pcr assay. | the protein disulfide isomerase (pdi) is a ubiquitous protein, which contributes in building disulfide bridges. in the work presented here, the expression of the pdi in different stages of the canine hookworm ancylostoma caninum was investigated. third-stage larvae (l3), adults, as well as serum-stimulated and hypobiotic l3 were used. for quantification of the pdi gene transcription, a real-time pcr was used establishing a hybridization probe (taqmantrade mark probes) for detection of pdi copy n ... | 2007 | 17762941 |
| strains of the dog hookworm, ancylostoma caninum, specific to the dog and to the cat. | | 1929 | 17797938 |
| characterization of beta-tubulin genes in hookworms and investigation of resistance-associated mutations using real-time pcr. | human hookworms (ancylostoma duodenale, necator americanus) are a major cause of malnutrition and anemia, particularly in children, and high worm burdens can lead to stunted growth and mental retardation. mass drug administration (mda) with benzimidazole (bz) anthelmintics has the potential to greatly reduce morbidity and infection prevalence. however, such treatment strategies may apply significant selection pressure on resistance alleles. in several strongylid parasites of livestock, resistanc ... | 2007 | 17850900 |
| impact of population structure on genetic diversity of a potential vaccine target in the canine hookworm (ancylostoma caninum). | ancylostoma caninum is a globally distributed canine parasitic nematode. to test whether positive selection, population structure, or both affect genetic variation at the candidate vaccine target ancylostoma secreted protein 1 (asp-1), we have quantified the genetic variation in a. caninum at asp-1 and a mitochondrial gene, cytochrome oxidase subunit 1 (cox-1), using the statistical population analysis tools found in the snap workbench. the mitochondrial gene cox-1 exhibits moderate diversity wi ... | 2007 | 17918358 |
| marburg virus angola infection of rhesus macaques: pathogenesis and treatment with recombinant nematode anticoagulant protein c2. | the procoagulant tissue factor (tf) is thought to play a role in the coagulation disorders that characterize filoviral infections. in this study, we evaluated the pathogenesis of lethal infection with the angola strain of marburg virus (marv-ang) in rhesus macaques and tested the efficacy of recombinant nematode anticoagulant protein c2 (rnapc2), an inhibitor of tf/factor viia, as a potential treatment. | 2007 | 17940973 |
| gender-enriched transcription of activation associated secreted proteins in ostertagia ostertagi. | activation associated secreted proteins (asp) are members of a nematode-specific protein family belonging to the scp/tpx-1/ag5/pr-1/sc7 family. three different types of molecules have been identified in this family: two-domain asps and single-domain asps showing homology to either the c-terminal or n-terminal domain of the two-domain asp. the function of these proteins is still unclear, but a role in transition to parasitism and a role as allergen are often suggested. here we report that the abo ... | 2008 | 17961575 |
| echinococcosis in dogs in urban and rural areas in dakahlia governorate, egypt. | a sample of one hundred and ninety stray dogs was captured from mansoura city (urban) and three hundreds and fifty stray dogs were captured from meet el-korama, mansheit el-badawy villages (rural). the total prevalence of e. granulosus was 5%, with a worm burden ranging from 4 to 1010 (mean = 421). the significant prevalence was 6% in the rural area and 3.2% in the urban one. e. granulosus showed higher prevalence in young than old dogs and in males than females but without significant differenc ... | 2007 | 17985582 |
| the bandit, a new dna transposon from a hookworm-possible horizontal genetic transfer between host and parasite. | an enhanced understanding of the hookworm genome and its resident mobile genetic elements should facilitate understanding of the genome evolution, genome organization, possibly host-parasite co-evolution and horizontal gene transfer, and from a practical perspective, development of transposon-based transgenesis for hookworms and other parasitic nematodes. | 2007 | 17989781 |
| mitochondrial dna variation of the dog hookworm ancylostoma caninum in brazilian populations. | the mitochondrial cytochrome oxidase i gene was partially sequenced for 164 ancylostoma caninum individuals, originating from five different localities in brazil, with the aim of describing the genetic diversity and genetic structure of brazilian hookworm populations. allelic and nucleotide diversity were moderate (overall h=0.88 and pi=0.016) and were similar among cities. there was moderate genetic differentiation among the populations sampled (approximately phi(st)=0.12) and a weak but nonsig ... | 2008 | 17997042 |
| [solid medium-filter paper cultivation of ancylostoma caninum larvae]. | consisted of beef extract (3 grams), peptone (10 grams), sodium chloride (5 grams), agar (20 grams) and distilled water, the solid medium was used with filter paper for cultivation of the larvae of ancylostoma caninum. the method shows higher detection rate and simpler operation than the traditional test tube-filter paper culture technique. | 2007 | 18038814 |
| ancylostoma caninum infection in a texas-born blue lacy dog--alberta. | | 2007 | 18050802 |
| identification of stage-specific proteins of angiostrongylus vasorum (baillet, 1866) kamensky. | sera of ten dogs infected with angiostrongylus vasorum were analyzed by immunoblotting proteins of first stage larvae (l1) and adult parasites. the molecular weights (m.w.) of the principal l1 proteins identified by igg, igm, iga, and ige were 18-118 kda and those of the adult parasite were 28-209 kda. the l1 proteins had not been recognized by igg, igm, and iga antibodies in sera of dogs naturally infected with toxocara canis, ancylostoma caninum, and dipylidium caninum, although only weakly by ... | 2008 | 18060430 |
| the canine hookworm genome: analysis and classification of ancylostoma caninum survey sequences. | hookworms infect nearly a billion people. the ancylostoma caninum hookworm of canids is a model for studying human infections and information from its genome coupled with functional genomics and proteomics can accelerate progress towards hookworm control. as a step towards a full-scale a. caninum genome project, we generated 104,000 genome survey sequences (gsss) and determined the genome size of the canine hookworm. gsss assembled into 57.6 mb of unique sequence from a genome that we estimate b ... | 2008 | 18082904 |
| transcriptional changes in the hookworm, ancylostoma caninum, during the transition from a free-living to a parasitic larva. | third-stage larvae (l3) of the canine hookworm, ancylostoma caninum, undergo arrested development preceding transmission to a host. many of the mrnas up-regulated at this stage are likely to encode proteins that facilitate the transition from a free-living to a parasitic larva. the initial phase of mammalian host invasion by a. caninum l3 (herein termed "activation") can be mimicked in vitro by culturing l3 in serum-containing medium. | 2008 | 18235850 |
| application of in vitro anthelmintic sensitivity assays to canine parasitology: detecting resistance to pyrantel in ancylostoma caninum. | resistance of the canine hookworm ancylostoma caninum to anthelmintic therapy with pyrantel is an emerging problem in canine veterinary practice. detecting anthelmintic resistance in parasites of pets is problematic because traditional resistance-monitoring techniques used with livestock parasites, such as the faecal egg count reduction test, are often impractical for use in small animals. we used two field-collected isolates of a. caninum in an abbreviated critical trial to test their pyrantel ... | 2008 | 18242867 |
| helminth infections in faecal samples of wolves canis lupus l. from the western beskidy mountains in southern poland. | eighty-nine samples of grey wolf (canis lupus l.) faeces were collected between 2002 and 2004 from two areas in the western beskidy mts (south poland). helminth eggs were observed in 56.2% of faeces examined. these included: alaria alata (2.2%), taeniid eggs (11.2%), toxocara canis (5.6%), toxascaris leonina (1.1%), eucoleus aerophilus (14.6%), ancylostoma caninum (12.3%), uncinaria stenocephala (37%) and unidentified roundworm eggs of the family strongyloididae (1.1%). eucoleus aerophilus is re ... | 2007 | 18251025 |
| a novel small heat shock protein 12.6 (hsp12.6) from brugia malayi functions as a human il-10 receptor binding protein. | phage display cdna expression library of the third stage larvae (l3) of brugia malayi was screened for identifying target(s) that bound to the human interleukin-10 receptor (huil10r). this iterative screening identified an insert that showed significant homology to caenorhabditis elegans hsp12.6. the gene was designated b. malayi hsp12.6 (bmhsp12.6) and has orthologues in several gastrointestinal nematode genome (ancylostoma caninum, ascaris lumbricoides and ascaris suum) but the gene or gene pr ... | 2008 | 18395809 |
| pcr-based coprodiagnostic tools reveal dogs as reservoirs of zoonotic ancylostomiasis caused by ancylostoma ceylanicum in temple communities in bangkok. | a survey of gastrointestinal parasites of dogs and humans from temple communities in bangkok revealed that 58% of dogs and 3.4% of humans, among those sampled, were infected with hookworms utilising faecal flotation techniques and microscopy. a previously established polymerase chain reaction (pcr)-rflp approach was utilised to determine the species of hookworms infecting dogs found positive for hookworm eggs. single infections with ancylostoma ceylanicum and ancylostoma caninum were recorded in ... | 2008 | 18556131 |
| plant vermicides of haitian vodou show in vitro activity against larval hookworm. | haitian vodou priests (houngans) and priestesses (mambos) use plant remedies to treat many illnesses, including intestinal parasite infections. the present study screened 12 plants used in vodou treatments for intestinal parasites to detect in vitro activity against infective-stage larvae of the hookworm ancylostoma caninum. water-soluble extracts of 4 of the 12 plants inhibited serum-stimulated feeding by larval a. caninum in a dose-dependent manner. all 4 plant extracts inhibited feeding induc ... | 2008 | 18576795 |
| strategies for the storage of ancylostoma caninum third-stage larvae. | although cryopreservation protocols for storage of hookworm larvae have been described, the circumstances under which the technique is necessary to ensure larval survival are not well defined. the motility of infective-stage larvae (as judged by observation) and their ability to migrate through canine skin in vitro were measured over a 7-mo period in worms held at room temperature and worms that had been cryopreserved at the start of the experiment. cryopreserved worms showed motility and migrat ... | 2008 | 18605779 |
| encroachment of echinococcus granulosus into urban areas in eastern queensland, australia. | to investigate the prevalence of echinococcus granulosus in wild dogs (dingos and dingo-domestic dog hybrids) living in and around human habitation on fraser island and in townships of the maroochy shire, on queensland's sunshine coast, australia. | 2008 | 18673469 |
| phenotypic characterization of two ancylostoma caninum isolates with different susceptibilities to the anthelmintic pyrantel. | the anthelmintic pyrantel plays an important role in the control of gastrointestinal helminths of humans and domestic animals. despite the demonstration of pyrantel resistance in several helminth species over the last 20 years, the resistance mechanism remains unclear. it has been hypothesized that resistance may arise as a consequence of changes to the relative proportions of subpopulations of nicotinic acetylcholine receptors (nachrs). to test this hypothesis, we examined the responses of two ... | 2008 | 18710918 |
| proteomics analysis of the excretory/secretory component of the blood-feeding stage of the hookworm, ancylostoma caninum. | hookworms are blood-feeding intestinal parasites of mammalian hosts and are one of the major human ailments affecting approximately 600 million people worldwide. these parasites form an intimate association with the host and are able to avoid vigorous immune responses in many ways including skewing of the response phenotype to promote parasite survival and longevity. the primary interface between the parasite and the host is the excretory/secretory component, a complex mixture of proteins, carbo ... | 2009 | 18753127 |
| cytosolic glutathione s-transferases of oesophagostomum dentatum. | oesophagostomum dentatum stages were investigated for glutathione s-transferase (gst) expression at the protein and mrna levels. gst activity was detected in all stages (infectious and parasitic stages including third- and fourth-stage larvae of different ages as well as males and females) and could be dose-dependently inhibited with sulfobromophthalein (sbp). addition of sbp to in vitro larval cultures reversibly inhibited development from third- to fourth-stage larvae. two glutathione-affinity ... | 2008 | 18771610 |
| investigations into the prevention of neonatal ancylostoma caninum infections in puppies by application of imidacloprid 10% plus moxidectin 2.5% topical solution to the pregnant dog. | the aim of the investigation was to examine whether a single topical administration of a combination of imidacloprid and moxidectin to pregnant dogs could prevent neonatal infections with reactivated ancylostoma caninum larvae. three pregnant beagles, infected with a. caninum, were treated topically with the combination on day 56 of pregnancy. three further dogs served as untreated controls. treatment appeared to prevent neonatal infections in the puppies completely. neither intestinal stages no ... | 2009 | 18793274 |
| molecular cloning and characterization of ac-tmp-2, a tissue inhibitor of metalloproteinase secreted by adult ancylostoma caninum. | ac-tmp-2, an immunodominant hookworm antigen encoding a tissue inhibitor of metalloproteinase (timp) was cloned by immunoscreening an ancylostoma caninum larval cdna library with sera pooled from dogs immunized with irradiated a. caninum third stage larvae (ir-l3). the open reading frame of ac-tmp-2 cdna encoded a 244 amino acids (predicted molecular weight of 27.7 kda), which shared a common n-terminus with other vertebrate and invertebrate timps, including ac-tmp-1, the most abundant adult hoo ... | 2008 | 18804124 |
| [helminths of pampas fox, pseudalopex gymnocercus (fischer, 1814) and of crab-eating fox, cerdocyon thous (linnaeus, 1766) in the south of the state of rio grande do sul, brazil]. | forty wild canids were captured by live trap at municipalities of pedro osorio and pelotas in southern of the state of rio grande do sul and they were transported to the parasitology laboratory at the universidade federal de pelotas. after they were posted, segments of intestinal, respiratory and urinary tracts and liver were separated and examined. animal skulls were used for taxonomic identification. of forty wild animals trapped, 22 (55%) were pseudalopex gymnocercus and 22 (55%) cerdocyon th ... | 2008 | 18823576 |
| acetylcholine receptor subunit genes from ancylostoma caninum: altered transcription patterns associated with pyrantel resistance. | the molecular mechanism of resistance to nicotinic agonist anthelmintics such as pyrantel and levamisole in nematodes of medical and veterinary significance is poorly understood. the identification of pyrantel-resistant isolates of the canine hookworm, ancylostoma caninum, provides an opportunity to explore, at a molecular level, the mechanism of cholinergic resistance in a species that is a model for the human hookworms. here we describe the cloning of three a. caninum genes orthologous to comp ... | 2009 | 18823982 |
| recent research developments in the direct inhibition of coagulation proteinases--inhibitors of the initiation phase. | physiologic clotting is a defensive action. the new cell-based model of hemostasis proposes three steps--initiation, amplification and propagation--occurring on specific cell surfaces to generate a thrombus in a tightly regulated manner. the initiation phase relies on key players including tissue factor (tf), factor viia (fviia), platelets, ca2+, phospholipids, and factor x/xa (fx/fxa). exposure of tf on sub-endothelial and other blood cells triggers a coagulation response, which may have to be ... | 2008 | 18855645 |
| molecular cloning and dna binding characterization of daf-16 orthologs from ancylostoma hookworms. | infective hookworm l3s encounter a host-specific signal during infection that re-initiates a suspended developmental pathway, resulting in development to the adult stage. this resumption of development in the host is analogous to recovery of developmentally arrested caenorhabditis elegans dauer larvae in response to favorable environmental signals. dauer recovery in c. elegans dauers and hookworm l3s is mediated by insulin-like signaling (ils). a key output of ils in c. elegans is the forkhead t ... | 2009 | 18930062 |
| exploring transcriptional conservation between ancylostoma caninum and haemonchus contortus by oligonucleotide microarray and bioinformatic analyses. | in this study, we identified, using an established oligonucleotide microarray platform for the parasitic nematode haemonchus contortus, transcripts that are 'conserved' between serum-activated and non-activated l3s of ancylostoma caninum (al3 and l3, respectively) and h. contortus by cross-species hybridization (csh) at high stringency and conducted extensive bioinformatic analyses of the cross-hybridizing expressed sequence tags (ests). the microarray analysis revealed significant differential ... | 2009 | 18977290 |
| therapeutic potential of helminth soluble proteins in tnbs-induced colitis in mice. | the hygiene hypothesis suggests an inverse relationship between the incidence of parasitic infections and chronic inflammatory bowel diseases (ibd). we investigated the therapeutic potential of schistosoma mansoni and ancylostoma caninum soluble proteins on experimental colitis in mice. | 2009 | 19023900 |
| investigation of the regulation of transcriptional changes in ancylostoma caninum larvae following serum activation, with a focus on the insulin-like signalling pathway. | the exit from dauer in the free-living nematode caenorhabditis elegans is under the control of a single amphidial neuron (asj) of the insulin-like signalling pathway. mutations of this pathway have the ability to suppress entry into the dauer stage. it has been postulated that insulin-like signalling plays a significant role in the response to serum stimulation in vitro of the third-stage larvae (l3s) of the canine hookworm ancylostoma caninum. to test for the possible involvement of the insulin ... | 2009 | 19054616 |