| ionic effects on the structure of nucleoprotein cores from adenovirus. | nucleoprotein cores, prepared from adenovirus type 5 with a deoxycholate/heat treatment, consist of the viral dna and two major internal proteins. the core particles exhibit structural characteristics that are highly reproducible and dependent on their ionic environment. in low-ionic-strength buffer, the cores had a sedimentation coefficient of 180 s and appeared in the electron microscope as homogeneous particles with distinct centers from which numerous arms and loops radiated. condensation of ... | 1985 | 4041462 |
| an association between replicating adenovirus dna and the nuclear matrix of infected hela cells. | an association between newly synthesized human adenovirus type 5 dna and the nuclear matrix of infected hela cells is described. adenovirus-infected cells were pulsed labeled with [3h]thymidine late in infection and the nuclear matrix was prepared. after a 1-min pulse more than 95% of the labeled viral dna was matrix associated and, when compared with total cell dna, was resistant to dnase i digestion. when the pulse is longer or is followed by a chase period, the viral dna remains nuclear matri ... | 1985 | 4041965 |
| adenovirus 5 early region 1a host range mutants hr3, hr4, and hr5 contain point mutations which generate single amino acid substitutions. | the early region 1a (e1a) gene of adenovirus 5 encodes two proteins, 289aa and 243aa, which are translated from mrnas of 13s and 12s, respectively. these two e1a proteins are identical except for an internal stretch of 46 amino acids unique to the larger protein. the 289aa protein activates transcription from promoters of other early adenoviral genes. the adenovirus type 5 host range mutants hr3, hr4, and hr5 are unable to activate transcription of these early viral genes. we show here that hr3, ... | 1985 | 3861875 |
| size and location of the transforming region in human adenovirus type 5 dna. | | 1974 | 4473721 |
| analysis of multiple forms of human adenovirus type 5 e1a polypeptides using an anti-peptide antiserum specific for the amino terminus. | studies were carried out to further characterize the proteins coded for by the early 1a region (e1a) of human adenovirus type 5 (ad5) in lytically infected cells using an antiserum prepared against a synthetic octapeptide corresponding to the amino termini of e1a products as well as another anti-peptide serum specific for the carboxy termini. both sera precipitated the same collection of four major and two minor acidic phosphoproteins and it was confirmed that each of the 1.1- and 0.9-kb e1a mrn ... | 1985 | 4049735 |
| individual adenovirus type 5 early region 1a gene products elicit distinct alterations of cellular morphology and gene expression. | adenovirus early region 1a (e1a), which gives rise to three overlapping transcripts, was inserted into a murine leukemia virus-derived vector, and recombinant viruses were used to prepare permanent cell lines of nih 3t3 cells containing dna copies of the individual 13s, 12s, and 9s mrnas. integrated proviral copies of the recombinant genomes were rescued as bacterial plasmids from each of the cell lines, and the dna sequence of e1a was demonstrated to be a precise copy of the individual transcri ... | 1985 | 4057356 |
| stabilities and interrelations of multiple species of human adenovirus type 5 early region 1 proteins in infected and transformed cells. | early region 1a (e1a) of human adenovirus type 5 (ad5) produces two mrnas coding for phosphoproteins of 289 and 243 residues (289r and 243r). each of these products has been shown to migrate on sodium dodecyl sulfate gels as two major and two minor species. in the present study, the stabilities of e1a polypeptides, as well as those of some other early ad5 proteins, were studied in infected kb cells that were pulse-labeled with [35s]methionine and then chased in the presence or absence of cyclohe ... | 1985 | 4057361 |
| replication of adenovirus types 5, 7, and 12 during inhibition of host cell oxidative metabolism. | replication of human adenovirus type 5 (non-oncogenic), type 7 (weakly oncogenic), and type 12 (highly oncogenic) was studied. inhibition of cellular oxidative metabolism with sodium cyanide resulted in much lower yields of progeny virions in chimpanzee liver cells, an established cell line derived by biopsy from a normal chimpanzee. inhibition of oxidative metabolism had no effect on virus replication in hep-2 cells, an established cell line derived from epidermoid carcinoma tissue from the lar ... | 1985 | 4058322 |
| species dependence of the major late promoter in adenovirus type 12 dna. | in hamster cells human adenovirus type 12 (ad12) is deficient in dna replication and late gene expression whereas adenovirus type 2 (ad2) can replicate. functions located in the e1 region of the ad2 or adenovirus type 5 (ad5) genome can complement the deficiencies of the ad12 genome in hamster cells, but, infectious viral particles are not produced. we have now investigated the activity of the major late promoter of ad2 and of ad12 dna in human and hamster cells. this promoter governs the expres ... | 1985 | 4065097 |
| properties of adenoviral dna bound to the nuclear matrix. | the association of adenoviral dna with the high salt (2 m nacl) resistant nuclear fraction, termed the nuclear matrix, has been investigated in hela cells at different times after infection with adenovirus type 5. when nuclear matrices were prepared in the absence of exogenously added nucleases, ad5 dna was quantitively associated with the matrix throughout the infection period (0-24 h). moreover, early in infection (0-10 h) ad5 dna was severalfold enriched in dnase i digested nuclear matrices ( ... | 1985 | 4096901 |
| rapid detection and identification of respiratory viruses by direct immunofluorescence. | the use of fluorescein-conjugated antiserum against respiratory syncytial (rs) and parainfluenza 1 and 3 viruses was compared with conventional techniques in the rapid detection of virus in tissue cultures inoculated with pharyngeal specimens known to contain these viruses. twenty-three specimens were tested: 9 rs, 8 parainfluenza 1, and 6 parainfluenza 3. the fluorescent-antibody technique (fa) detected virus in 52% of the tissue cultures in 24 hr, and, by 72 hr, 22 of the 23 cultures were fa-p ... | 1970 | 4098101 |
| modification of dopa toxicity in human tumour cells. | a variety of factors were found to modify the toxicity of l-dopa in hela cells (d37 16 microm) and in dopa-sensitive, nonpigmented human melanoma cells (mm96) (d37 5 microm) having a similar size and doubling time. dopa toxicity was decreased by concurrent treatment with superoxide dismutase, peroxidase or catalase, by erythrocytes, or by hypoxia. toxicity could be increased by the enzyme inhibitors l- and d-penicillamine, sodium diethyldithiocarbamate or 3-amino-1,2,4-triazole. the two cell lin ... | 1985 | 3924049 |
| semipermissive replication of adenovirus 5 in rat brain cells and evidence for an induction of cellular dna replication in vivo. | human adenovirus type 5 is shown to cause a limited infection of the cells lining the ventricles of 2- to 3-week-old newborn rats. by the distribution of fluorescent antibody-labelled cells the infection appears to be confined to the ependymal cells. in these same (ependymal) cells autoradiographic labelling of [3h]thymidine-pulsed brains indicates that viral and possibly cellular dna synthesis is occurring. studies with the mutants dl 312 and dl 313 indicate that some cellular dna synthesis is ... | 1986 | 3944589 |
| [action of triton wr-1339 and deae-dextran on the multiplication of adenovirus 5 in cell cultures]. | | 1971 | 4261490 |
| [a case of facial paralysis caused by adenovirus type 5 infection]. | | 1966 | 4285741 |
| the virus watch program. iv. recovery and comparison of two serological varieties of adenovirus type 5. | | 1966 | 4288639 |
| in vitro immune responses of rabbits with persistent adenovirus type 5 infection. | | 1969 | 4310737 |
| [interference-microscopy dry-mass studies on the nucleus, plasma and nucleolus of diploid mouse embryonal cells infected with adenovirus type 5]. | | 1973 | 4352138 |
| induction of immunity in man by crystalline adenovirus type 5 capsid antigens. | | 1973 | 4355246 |
| persistence of adenovirus 5 in guinea pigs. | one intracardiac inoculation of adenovirus 5 in guinea pigs leads to virus persistence in different organs, viz., 5 days in lungs and liver, 14 days in blood and lymph nodes, and 56 days or more in the spleen. after cultivation of tissue cells for 1 week, virus was recovered from blood, lymph nodes, or spleen lymphocytes, but virus could be detected directly in cells only when organs were removed within 48 h of inoculation. to determine how the virus persisted in low concentrations and as a late ... | 1974 | 4366914 |
| antibodies against adeno-, cytomegalo and rubella viruses in australia-antigen-negative sera from patients with infectious hepatitis. | antibodies neutralizing adenovirus type 5 were found in all of 50 pairs (100%) of sera from patients with acute icteric infectious hepatitis. the incidence in sera from the general population was 57%. no differences in mean titre or in proportion of positive sera were found in the same sera tested for complement-fixing antibodies to cytomegalovirus and for antibodies to rubella virus haemagglutinin. the results can be interpreted as supporting the involvement, either direct or indirect, of adeno ... | 1974 | 4367226 |
| [action of actinomycin d, puromycin, and p-fluorophenylalanine on the thermosensitive phase of the multiplication of adenovirus 5]. | | 1968 | 4971305 |
| adsorption and fate of adenovirus type 5 hexon antigen in hela cells. | | 1969 | 4983062 |
| the activity of oxidative enzymes in detroit-6 cells infected with adenovirus type 5. a cytochemical study. | | 1966 | 4380764 |
| effect of -amanitin on adenovirus 5 multiplication. | | 1972 | 5062858 |
| the effect of pre-inhibition of dna synthesis in continuous human amniotic cell culture (strain fl) on adenovirus type 5 reproduction. | | 1969 | 4398699 |
| solubilization and fractionation of glycoproteins and glycolipids of kb cell membranes. | 1. a fraction enriched in plasma membranes of human tumour kb cell line, a permissive cell for adenovirus type 5, was obtained. 2. electrophoresis of the membranes in polyacrylamide gels with buffers containing sodium dodecyl sulphate showed that the membranes after reduction with 2-mercaptoethanol contained over 20 polypeptide species. three polypeptides were glycosylated and had apparent mol.wts. of 92000, 72000 and 62000. 3. the glycoproteins and the specific receptors responsible for adenovi ... | 1974 | 4447626 |
| [morphology and polypeptides of complete and incomplete adenovirus type 5 particles (author's transl)]. | | 1974 | 4463759 |
| mixed infections of kb cell cultures with type 5 adenovirus and vaccinia virus. ii. effect of adenovirus type 5 on the kinetics of development of vaccinia virus. | | 1972 | 4511760 |
| the immunogenicity of adenovirus type 5 structural proteins. | | 1972 | 4626042 |
| characterization of temperature-sensitive mutants of adenovirus type 5--serology. | | 1972 | 4631143 |
| isolation of conditional lethal mutants (temperature sensitive and host-dependent mutants) of adenovirus type 5. | | 1972 | 4672419 |
| a new technique for the assay of infectivity of human adenovirus 5 dna. | | 1973 | 4705382 |
| oncogenic transformation of hamster embryo cells in vitro by adenovirus type 5. | | 1973 | 4706286 |
| characterization of temperature-sensitive mutants of adenovirus type 5: nucleic acid synthesis. | | 1973 | 4713039 |
| [interference microscopic dry mass determination of primary struma cells in tissue culture. 3. effect of infection with adenovirus type 5]. | | 1973 | 4730765 |
| the purification of adenovirus neutralizing antibody: adenovirus type 5 hexon immunoadsorbent. | | 1972 | 4110983 |
| [replication of adenovirus type 5 in culture of cord blood lymphocytes (author's transl)]. | | 1974 | 4143244 |
| study of adenovirus type 5 antigens by immuno-osmophoresis. | | 1967 | 4232907 |
| studies on the mechanism of replication of adenovirus dna. ii. the nature of single-stranded dna in replicative intermediates. | replicative intermediates of adenovirus type 5 dna contain large stretches of single-stranded dna. we have shown that this single-stranded dna is mainly of parental origin, whereas all new dna synthesized during one round of replication has a double-stranded structure. hybridization experiments of the single-stranded dna with isolated complementary strands of adenovirus type 5 dna showed that this dna hybridized only with the viral l-strand (the strand with the lower equilibrium density in alkal ... | 1973 | 4765398 |
| crystallographic study of intranuclear adenovirus type 5 crystals. | | 1974 | 4823536 |
| beta-glucuronidase response of cells infected with adenovirus types 5 and 12. | beta-glucuronidase activity was investigated during a 48-h period in which virus replication and changes in cell morphology occurred. infection of an established line of chimpanzee liver cells with either nononcogenic adenovirus 5 or highly oncogenic adenovirus 12 under one-step growth conditions produced differing patterns of enzyme activity. there was an increase in total activity and also enhanced leakage of beta-glucuronidase from cells infected with adenovirus 12. in contrast, the enzymatic ... | 1974 | 4842701 |
| characterization of temperature-sensitive mutants of adenovirus type 5: synthesis of polypeptides in infected cells. | | 1974 | 4850983 |
| transformation of a hamster embryo cell line (nil) with a host-dependent mutant of adenovirus type 5. | | 1974 | 4856428 |
| [study of the synthesis of adenovirus type 5 and type 7 in hela cell cultures]. | | 1968 | 4893546 |
| studies on infectious mononucleosis: i. cytopathic agents and immunofluorescence studies. | viral studies were carried out on throat swabs, rectal swabs and washed white blood cells from 27 cases of infectious mononucleosis (positive paul-bunnell-david-sohn test), and from 22 controls.four cytopathic agents were isolated in the test group, two of which were readily subcultured for at least three successive passages.three cytopathic agents were recovered in the control group, two of which have been identified as adenovirus type 5 and adenovirus type 3. the unidentified agents tested so ... | 1966 | 4952899 |
| the proteins of adenovirus type 5. | | 1970 | 4989118 |
| differential susceptibility of two cell types to the chromosome-breaking effects of adenovirus type 5. | | 1971 | 5094806 |
| complementation and recombination with temperature-sensitive mutants of adenovirus type 5. | | 1971 | 5157831 |
| temperature-restricted mutants of human adenovirus type 5. in: strategy of the viral genome. | | 1971 | 5211751 |
| succinic dehydrogenase activity in tissue cultures infected with adenovirus type 5. | | 1965 | 5213779 |
| investigation on intranuclear paracrystalline inclusions induced by adenovirus 5 in kb cells. | | 1970 | 5437472 |
| origin of thymidine kinase in adenovirus-infected human cell lines. | human adenovirus type 5 enhances the thymidine kinase activity of kb cells but does not induce the enzyme in kinase-deficient hela (bu25) cells. vaccinia induces thymidine kinase activity in both kb and hela (bu25) cells. human adenovirus types 2, 4, 7, and 12 also fail to induce the enzyme in hela (bu25) cells. vaccinia replicates equally well in the presence or absence of hatg (hypoxanthine-aminopterin-thymidine-glycine) in kb and hela (bu25) cells. adenovirus type 5 replicates in kb and in he ... | 1970 | 5452502 |
| isolation of temperature-sensitive mutants of adenovirus type 5. | | 1971 | 5564419 |
| adenovirus soluble antigen vaccine studies. i. preparation and testing of adenovirus type 5 soluble antigen vaccines. | | 1968 | 5668108 |
| isolation of an internal component from adenovirus type 5. | | 1968 | 5719215 |
| the effect of nonaqueous solvents on the quaternary structure of viruses: disruption of adenovirus type 5 by formamide. (brief report). | | 1969 | 5782936 |
| crystallographic data for the hexon of adenovirus type 5. | | 1969 | 5788980 |
| transformation of rat cells by dna of human adenovirus 5. | | 1973 | 4737663 |
| mixed infections of kb cell cultures with type 5 adenovirus and vaccinia virus. ii. effect of adenovirus type 5 on the kinetics of development of vaccinia virus. | | 1973 | 4739551 |
| adenovirus in blood clots from cases of infectious hepatitis. | adenovirus type 5 was isolated from blood clots from 27 of 30 sporadic cases of infectious hepatitis. only one isolation of virus, also adenovirus 5, was made from blood clots from 70 persons with no known contact with infectious hepatitis. | 1966 | 5937134 |
| specific transplantation antigens of mouse sarcomas induced by adenovirus type 12. | a specific isograft resistance against three different mouse adeno 12 sarcomas was demonstrated in mice treated with four to eight doses of viable or x-ray-killed adeno 12 mouse tumors. whole-body x-ray irradiation with 350 r 24 hr previous to the test challenge did not abolish the resistance, indicating that it was due to a true anamnestic immune reaction. this was further proven by the finding that similar treatment with tumors of other origin did not induce any immunity, nor did the treatment ... | 1967 | 6020008 |
| a new animal model for human respiratory tract disease due to adenovirus. | cotton rats (sigmodon hispidus) were tested as a model for human respiratory tract infection due to adenovirus. after intranasal instillation of 10(6.1) 50% tissue culture infectious doses (tcid50) of adenovirus type 5 into one-month-old cotton rats, groups were killed at intervals for nasal and lung titration of virus and lung histopathology. in lung, eclipse occurred at 8 hr followed by peak viral titer (10(7.5) tcid50/g of lung) on day 5. titers fell to 10(3.2) tcid50/g by day 10 and persiste ... | 1984 | 6086773 |
| pppa2'p5a' blocks vesicular stomatitis virus replication in intact cells. | pppa2'p5'a blocked the production of infectious vesicular stomatitis virus in hela cells. when this compound was present from the beginning of infection, a selective inhibitory effect was observed in viral protein synthesis. thus, cellular translation was not affected even after 10 h of incubation with this compound, and the bulk of viral proteins was not synthesized. however, this effect was not observed with atp, gtp, or the core a2'p5'a. the step blocked by pppa2'p5'a is located early during ... | 1984 | 6090695 |
| complementation of an adenovirus 5 immediate early mutant by human cytomegalovirus. | the initiation of the lytic cycle of adenovirus 5 requires synthesis of a transcriptional activator encoded by the viral early genetic unit, e1a. mutant viruses lacking e1a are defective. human cytomegalovirus activated transcription of early adenovirus genes and complemented an e1a- adenovirus mutant in cells permissive for both viruses. | 1984 | 6091338 |
| the nucleotide sequence of the leftmost xhoi fragment (6%) of simian adenovirus sa7p. | the dna of simian adenovirus sa7p was cloned in pbr322. the nucleotide sequences of the leftmost 2238 bp and the rightmost 188 bp of the viral genome were determined. sa7p dna has an inverted terminal repeat of 183 bp. the sequence at the left terminus exhibits extensive homology with that of the e1 regions of human adenovirus 5, 7 and 12 dnas. based on this homology, the rna coordinates and coding regions could be deduced. the sequenced sa7p dna contains the entire e1a and part of the e1b regio ... | 1984 | 6092520 |
| dna-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses. | chick cells infected by chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contained four prominent virus-specific, structurally related dna-binding proteins with mol. wt. of 74k, 64k, 56k, 52k, and two minor forms. the celo virus dna-binding proteins were phosphorylated, delayed-early nuclear proteins. celo virus early proteins were expressed in bhk cells, but did not complement human adenovirus type 5 mutants with lesions e1a, e2a or e2b. moreover, celo virus dna-binding proteins ... | 1984 | 6092525 |
| efficient transformation of human fibroblasts by adenovirus-simian virus 40 recombinants. | the origin-defective simian virus 40 (sv40) mutant 6-1 has been useful in transforming human cells (small et al., nature [london] 296:671-672, 1982; nagata et al., nature [london] 306:597-599, 1983). however, the low efficiency of transformation achieved by dna transfection is a major drawback of the system. to increase the efficiency of sv40-induced transformation of human fibroblasts, we used recombinant adenovirus-sv40 virions which contain a complete sv40 early region including either a wild ... | 1984 | 6092928 |
| covalently closed circles of human adenovirus dna are infectious. | replication of the linear adenovirus dna molecule is thought to result from semiconservative synthesis off linear templates, starting from origins at either end of the genome. recently, however, it has been shown that in cells infected with adenovirus type 5 (ad5) a significant fraction of the ends of viral dna molecules become joined head-to-tail due at least in part to the formation of covalently closed circles. circular dna is not present in virions but joining of the ends of viral dna is det ... | 1984 | 6098454 |
| construction of human cell lines which contain and express the adenovirus dna binding protein gene by cotransformation with the hsv-1 tk gene. | we have introduced the dna binding protein (dbp) gene of human adenovirus type 5 (ad5) into high molecular weight dna of permissive human cells by cotransformation of tk- cells with the cloned dbp and hsv-1 thymidine kinase genes. 110 tk+ cell lines were isolated after selection in hat medium. the amount and arrangement of adenovirus sequences in the tk+ cell lines were analyzed by restriction endonuclease digestion and filter hybridization. twelve of the 110 lines carry at least a segment of th ... | 1984 | 6099656 |
| murine teratocarcinoma: a model for virus-cell interaction in a differentiating cell system. | the stem cell of the murine teratocarcinoma is refractory to infection with simian virus 40 and polyoma. utilizing various procedures, we attempted to alter this block to infection by modifying the infection procedure. multiple infections with high-titer sv40 and pretreatment of cells with deae-dextran or the carcinogen 4-nitroquinoline 1-oxide did not induce embryonal carcinoma cells to produce t-antigen. co-infection with adenovirus 5, which infects the embryonal carcinoma, and sv40 did not in ... | 1981 | 6100956 |
| an outbreak of respiratory disease due to a type 5 adenovirus identified as genome type 5a. | a cluster of adenovirus type 5-caused acute respiratory diseases was observed in prague in the autumn-winter season of 1978/79. an analysis of viral dna by sequence-specific restriction enzymes in two out of 39 isolates revealed that these isolates, identical to each other, were clearly distinct from adenovirus type 5 prototype strain. the designation "adenovirus genome type 5a" is suggested for these isolates. | 1980 | 6107033 |
| propagation and in vitro studies of previously non-cultivable enteral adenoviruses in 293 cells. | enteral adenovirus (ead) isolates which characteristically fail to propagate in several human cell lines grow readily in 293 cells, an adenovirus type 5 transformed line. ead infection of 293 cells is associated with high levels of 31s dna and late viral protein synthesis, the appearance of a progressive adenovirus-like cytopathic effect, and the efficient assembly of progeny virions. the dna of an ead strain grown in 293 cells possesses restriction endonuclease profiles similar to those reporte ... | 1981 | 6116953 |
| structure and organization of the gene coding for the dna binding protein of adenovirus type 5. | we have determined the nucleotide sequence of a region of adenovirus type 5 (ad5) dna located between map positions 61.7 and 71.4, which covers the gene form the 72 kd dna binding protein (dbp) and the sequence encoding the amino-terminal part of the 100 kd protein. sequence analysis of cdna copies of dbp mrna revealed the existence of two abundant species of spliced mrna molecules. one species consists of two short leader sequences from positions 75.2 (67 and 68 nucleotides long) and 68.8 (77 n ... | 1981 | 6117824 |
| interactions between initiating chemical carcinogens, tumor promoters, and adenovirus in cell transformation. | cell culture systems that respond to the combined action of initiating chemical carcinogens, tumor promoters, and transforming viruses represent useful model systems for studying the complex multifactor nature of the carcinogenic process. we have utilized both secondary rat embryo (2 degrees re) and a clonal population of established fischer rat embryo (cref) cells to study the effect of multiple agents on the process of adenovirus transformation. in the present review we summarize our investiga ... | 1980 | 6119815 |
| isolation and characterization of tropomyosin-containing microfilaments from cultured cells. | we have developed a new method for the rapid isolation of tropomyosin-containing microfilaments from cultured cells using anti-tropomyosin monoclonal antibodies. anti-tropomyosin monoclonal antibodies induce the bundle formation of microfilaments, which can be easily collected by low speed centrifugation. electron microscopic studies of the isolated microfilaments show periodic localization of tropomyosin along the microfilaments of nonmuscle cells with a 33-34 nm repeat. furthermore, the isolat ... | 1983 | 6133866 |
| adenovirus type 5 early region 1b gene product is required for efficient shutoff of host protein synthesis. | to determine the role adenovirus 5 early region 1b-encoded 21- and 55-kilodalton proteins play in adenovirus productive infection, mutants have been isolated which were engineered to contain small deletions or insertions at 5.8, 7.9, or 9.6 map units. by using an overlap recombination procedure involving h5dl314 (delta 3.7 to 4.6 map units) dna cleaved at 2.6 map units with clai and the adenovirus 5 xhoi-c (0 to 15.5 map units) fragment containing the desired mutation, viral mutants were isolate ... | 1984 | 6142122 |
| [multiplication of adenovirus type 5. (electron microscopic findings)]. | | 1964 | 5832430 |
| [development of adenovirus (type 5) infections in tissue cultures under the condition of dissociation of oxidation and phosphorylation processes]. | | 1965 | 5885361 |
| adenovirus antibody measured by the passive hemagglutination test. | lefkowitz, stanley s. (variety children's research foundation, miami, fla.), julia a. williams, bernard e. howard, and m. michael sigel. adenovirus antibody measured by the passive hemagglutination test. j. bacteriol. 91:205-212. 1966.-rabbits immunized intravenously with adenovirus type 5 antigen were tested for antibody titers by use of the passive hemagglutination test (pha). primary and secondary responses were studied, and the class of antibody was determined by means of density gradient ce ... | 1966 | 5903093 |
| studies on antiviral agents. i. relationship between chemical reactivity of sulfhydryl reagents and their inactivating activity of adenovirus type 5. | | 1966 | 5909031 |
| cis and trans activation of globin gene transcription in transient assays. | we examined the effects of the simian virus 40 enhancer sequence on transcription of cloned human alpha- and beta-globin genes shortly after their introduction into cultured mammalian cells. we find that (i) detectable transcription of the beta-globin gene but not the alpha-globin gene requires linkage to the enhancer; (ii) the enhancer increases the amount of beta-globin rna at least 100-fold but results in only a 5- to 10-fold increase in the amount of alpha-globin rna; (iii) plasmid replicati ... | 1983 | 6200874 |
| premature termination by human rna polymerase ii occurs temporally in the adenovirus major late transcriptional unit. | we have recently demonstrated pausing and premature termination of transcription by eucaryotic rna polymerase ii at specific sites in the major late transcriptional unit of adenovirus type 2 in vivo and in vitro. in further developing this as a system for studying eucaryotic termination control, we found that prematurely terminated transcripts of 175 and 120 nucleotides also occur in adenovirus type 5-infected cells. in both cases, premature termination occurs temporally, being found only during ... | 1984 | 6209554 |
| identification of intranuclear structures containing the 72k dna-binding protein of human adenovirus type 5. | immunocytochemical techniques have been applied to cells infected with human adenovirus type 5 in order to study the distribution of antigens related to the virus-encoded mr = 72000 (72k) dna-binding protein. in embedded cells 72k antigen was found to be associated with 'clear' fibrillar inclusions, in which viral dna replication is known to take place. "dense" fibrillar inclusions, which are thought to contain previously replicated viral dna, do not contain detectable levels of 72k antigen, sug ... | 1984 | 6236979 |
| patterns of viral dna integration in cells transformed by wild type or dna-binding protein mutants of adenovirus type 5 and effect of chemical carcinogens on integration. | the integration pattern of viral dna was studied in a number of cell lines transformed by wild-type adenovirus type 5 (ad5 wt) and two mutants of the dna-binding protein gene, h5ts125 and h5ts107. the effect of chemical carcinogens on the integration of viral dna was also investigated. liquid hybridization (c(0)t) analyses showed that rat embryo cells transformed by ad5 wt usually contained only the left-hand end of the viral genome, whereas cell lines transformed by h5ts125 or h5ts107 at either ... | 1980 | 6246266 |
| the nucleotide sequence of adenovirus type 5 early region e1: the region between map positions 8.0 (hindiii site) and 11.8 (smai site). | the nucleotide sequence of the region between map positions 8.0 (hindiii site) and 11.8 (smai site) of adenovirus type 5 (ad5) has been determined. together with the sequences reported earlier (van ormondt et al., 1978; maat and van ormondt, 1979) it encompasses the entire leftmost early region e1 of ad5 dna (4126 base pairs). the total sequence revealed a number of potential regulatory signals (promoter sites, ribosome binding sites, 3'-poly(a)-associated sequences), which confirm that region e ... | 1980 | 6250944 |
| construction of restriction enzyme fragment libraries containing dna from human adenovirus types 2 and 5. | restriction-fragment libraries containing adenovirus type 2 (ad2) dna have been constructed, using the pbr322 plasmid (bolivar et al., 1977) as a vector. clones have been isolated which contain all the hindiii fragments of ad2 dna except the terminal g- and k-fragments inserted into the hindiii cleavage site of the vector. all the 13 smai-fragments of ad2 dna were separately inserted into the psti site of the pbr322 vector after addition of homopolymeric poly(dg) tails to the fragments and poly( ... | 1980 | 6250946 |
| persistence of human adenovirus 5 in human cord blood lymphoblastoid cell lines transformed by epstein-barr virus. | lymphoblastoid cell lines derived from human cord blood leukocytes were persistently infected with human adenovirus 5. these cell lines expressed the epstein-barr nuclear antigen, but no other epstein-barr virus-related antigen. they continually produced infectious adenovirus 5 particles, but this production could be inhibited by the presence of specific neutralizing antibody to adenovirus 5. this suggests that the persistent infection might be due to the continual reinfection of susceptible cel ... | 1980 | 6253398 |
| isolation of a pathogenic strain of ovine adenovirus type 5 and a comparison of its pathogenicity with that of another strain of the same serotype. | | 1980 | 6253537 |
| predicted structure of two adenovirus tumor antigens. | early adenovirus type 2(ad2) mrna sequences have been cloned by using the pbr322 plasmid as a vector. two clones that include sequences from region e1b were identified and their dnas were characterized by hybridization, restriction enzyme cleavage, and dna sequence analysis. the results showed that the clones were derived from two different spliced mrnas. by combining our results with the established dna sequence for region e1b of the closely related adenovirus type 5[maat, j., van beveren, c.p. ... | 1980 | 6253988 |
| adenovirus terminal protein protects single stranded dna from digestion by a cellular exonuclease. | adenovirus 5 dna-protein complex is isolated from virions as a duplex dna molecule covalently attached by the 5' termini of each strand to virion protein of unknown function. the dna-protein complex can be digested with e. coli exonuclease iii to generate molecules analogous to dna replication intermediates in that they contain long single stranded regions ending in 5' termini bound to terminal protein. the infectivity of pronase digested adenovirus 5 dna is greatly diminished by exonuclease iii ... | 1980 | 6255444 |
| defective repair of alkylated dna by human tumour and sv40-transformed human cell strains. | we have identified a group of 8 (among 39) human tumour cell strains deficient in the ability to support the growth of adenovirus 5 preparations treated with n-methyl-n'-nitro-n-nitrosoguanidine (mnng), but able to support the growth of non-treated adenovirus normally. this deficient behaviour defines the mer- phenotype. strains having the mer- phenotype were found to arise from tumours originating in four different organs. relative to mer+ strains, mer- tumour strains showed greater sensitivity ... | 1980 | 6256643 |
| enhanced sensitivity of tumorigenic cells to rapid rounding induced by phenylalaninol. | this study describes a rounding reaction induced in mammalian cells by the addition of phenylalaninol. in the chinese hamster ovary tsh1 line the rounding occurred rapidly with a half time of 1 min at 25 mm phenylalaninol. after the removal of phenylalaninol, the rounding was reversed, leading to the reflattening of the cells with a half-time of 3.5 min. rounding was inhibited by dibutyryl-camp and testosterone, and reflattening by cytochalasin b. either in the case of the tsh1 line and its grow ... | 1980 | 6257353 |
| purification and partial characterization of a 33 000 molecular weight endonuclease associated with human adenovirus type 5. | an endonuclease activity has been purified from human adenovirus type 5 (had5) virions and had5-infected cell extracts. the endonuclease activity is associated with a monomeric protein of molecular weight approximately 33 000. the endonuclease activity is more active at ph 4.5 than ph 7.2. incubation of the enzyme at room temperature for periods of longer than 96 h results in a substantial increase in activity. the endonuclease activity is sensitive to edta at concentrations 10mm or greater but ... | 1980 | 6257357 |
| nucleotide sequence analysis of a region of adenovirus 5 dna encoding a hitherto unidentified gene. | the nucleotide sequence of a region located on the adenovirus type 5 genome between coordinates 59.9 and 62.5 has been determined. this region comprises the 3'-termini of mrnas coding for the hexon and the dna binding protein. the sequence reveals the presence of an open reading frame in r-strand transcripts downstream from the hexon termination codon. the protein predicted from this region has a mw of 23,068 daltons. based on the characteristics of the mutant ad2ts1, which maps between coordina ... | 1980 | 6258160 |
| the nucleotide sequence of the transforming early region e1 of adenovirus type 5 dna. | the sequence of the leftmost 11.3% of the non-oncogenic human adenovirus type 5 (ad5) dna has been determined. this segment contains the entire early region e1 of the ad5 genome which has been shown to be involved in in vitro transformation of non-permissive rodent cells (van der eb et al., 1980). from the dna sequence, and from the mrna sequence data obtained by perricaudet et al, (1979, 1980) for the e1 mrnas from the closely related adenovirus type 2 (ad2), it is possible to predict the prima ... | 1980 | 6260576 |
| subgenomic viral dna species synthesized in simian cells by human and simian adenoviruses. | dna synthesized after infection of simian tissue culture cells (bsc-1 or cv-1) with human adenovirus type 2 or 5 or with simian adenovirus 7 was characterized. it was demonstrated that as much as 40% of the virus-specific dna in nuclei of infected monkey cells consists of subgenomic pieces. no subgenomic viral dna species were detected in the nuclei of human (hela) cells infected with these adenovirus types. restriction analysis showed that these short viral dna molecules contain normal amounts ... | 1981 | 6261009 |
| mapping a eukaryotic promoter: a dna sequence required for in vivo expression of adenovirus pre-early functions. | this study defines a dna sequence upstream from the mrna cap site required for in vivo expression of the adenovirus 2 pre-early region. the adenovirus 2 pre-early region and flanking sequences were cloned in escherichia coli plasmid pbr322. derivatives of the plasmid lacking portions of the upstream viral dna sequence were constructed. an assay was devised to test the ability of these plasmid dnas to complement an adenovirus 5 mutant with a deletion in the pre-early region. plasmids that retaine ... | 1981 | 6262792 |
| cytochemical hybridization with fluorochrome-labeled rna. ii. applications. | the cytochemical detection of specific dna sequences by hybridization with fluorochrome-labeled rna and detection of the hybrids by fluorescence microscopy is described. rnas complementary to the dna of the kinetoplasts of crithidia luciliae (an insect trypanosome) or to adenovirus-5 (ad-5) dna were labeled with the hydrazine derivative of tetramethylrhodamine isothiocyanate (tritc). the specificity of the reactions between the complementary rnas labeled both with 3h and tetramethylrhodamine was ... | 1981 | 6166654 |
| monoclonal antibodies against adenovirus type 5: preparation and preliminary characterization. | eight different hybridoma cells lines producing monoclonal antibodies against the major antigens of human adenovirus type 5 have been obtained. they were selected by screening initial hybridomas by the fluorescent antibody technique followed by radioimmune precipitation and they reacted with hexon, penton, fibre and 100k polypeptides. five apparently different epitopes against the hexon antigen were detected showing a spectrum of activity against the hexons of other serotypes, suggesting that th ... | 1981 | 6171612 |