| fermentation product analysis in the identification of black-pigmented bacteria from periodontitis. | a quantitative procedure is described for the analysis of fermentation products of eight representative black-pigmented gram-negative anaerobic bacterial strains (porphyromonas gingivalis 381, porphyromonas gingivalis atcc 33277, prevotella intermedia atcc 25611, prevotella nigrescens atcc 33563, prevotella melaninogenica atcc 25845, prevotella denticola atcc 33185, and prevotella loescheii atcc 15930) from oral sites in humans, using gas-liquid chromatography. this procedure for the identificat ... | 1997 | 9345789 |
| the effect of age on the development of gingivitis. clinical, microbiological and histological findings. | in the present "experimental gingivitis" study, the response of the marginal gingiva to plaque formation was studied in one group of young subjects, 20-25 years of age, and in one group of older subjects, aged 65-80 years. during a 4-week period, all subjects received a series of professional tooth cleaning to establish healthy gingival conditions. a baseline examination (day 0) included assessments of plaque and gingivitis. sites that were examined (the experimental sites) included the mesio-pa ... | 1996 | 8739171 |
| systemic antimicrobial treatment and guided tissue regeneration. clinical and microbiological effects in furcation defects. | the purpose of this investigation was to study the microbiota associated with furcation-involved teeth before and after treatment by the guided tissue regeneration procedure (gtr) with non-resorbable eptfe membranes, and to evaluate the benefit of additional systemic antimicrobial therapy (ornidazole). each of 10 patients contributed 1 pair of bilateral mandibular molars with comparable furcation defects. 5 defects were treated with a membrane and the active drug, 5 were treated without a membra ... | 1996 | 8739172 |
| anti-bacterial action of onion (allium cepa l.) extracts against oral pathogenic bacteria. | in this study, the focus was on the antibacterial activity of onions. this study researched the activities of onion extracts on streptococcus mutans and streptococcus sobrinus, the main causal bacteria for dental caries, and porphyromonas gingivalis and prevotella intermedia, considered to be the main causal bacteria of adult periodontitis. the results showed that the onion extracts possess an effect on all test bacterial strains (s.mutans jc-2, s. sobrinus omz176, p. gingivalis atcc 33277 and p ... | 1997 | 9354029 |
| putative periodontal pathogens in subgingival plaque of young adults with and without early-onset periodontitis. | this study assessed the associations between putative periodontal pathogens and early-onset periodontitis (eop) in a population of 248 subjects, 13 to 19 years of age at baseline, derived from a representative sample of u.s. young adults. the subjects were selected based on the presence or absence of attachment loss at baseline. the attachment level was assessed clinically at baseline and at a 6-year follow-up examination, and the presence of 7 bacterial species was assessed at follow-up using d ... | 1997 | 9358364 |
| distribution and mobility of the tetracycline resistance determinant tetq. | we tested 34 american type culture collection (atcc) and 168 clinical bacterial isolates, from the human urogenital and oral tracts and streptococci isolated from cows with mastitis, for the presence of the tetq gene using a polymerase chain reaction (pcr) assay and dna-dna hybridization. the identities of pcr products were confirmed by southern blot hybridization of whole-cell dna. eleven of the atcc strains were positive for tetq, including five bacteroides spp., five prevotella spp. and a sin ... | 1997 | 9372425 |
| experimental gingivitis in periodontitis-susceptible subjects. | the purpose of this study was to evaluate clinical, microbiological, and gingival crevicular fluid (gcf) profiles in periodontitis-resistant and periodontitis-susceptible subjects during 4 weeks of experimental gingivitis. experimental groups of similar ages were defined as gingivitis controls (gc; n = 10) and history of rapidly progressive periodontitis (rpp; n = 10), respectively. prior to baseline, all subjects achieved good plaque control (plaque index (p1i) approximately 0) and gingival hea ... | 1997 | 9378832 |
| colonization by actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in adult periodontitis patients as detected by the antibody-based evalusite test. | studies were performed to evaluate the detection of disease-associated bacterial colonization in adult periodontitis patients by the antibody-based evalusite testtm (eastman kodak company). the association of test results with disease was assessed by collecting 104 duplicate subgingival plaque samples from 26 patients. samples were tested for actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia using both microbiological culture and the immunoassay test. the s ... | 1996 | 8783055 |
| "checkerboard" versus culture: a comparison between two methods for identification of subgingival microbiota. | the present study compared the "checkerboard" dna-dna hybridization methodology with culture techniques for the analysis of the composition of the subgingival microbiota. 70 subjects, presenting with a variety of periodontal conditions, contributed with a total of 283 subgingival plaque samples analyzed with respect to the following species: porphyromonas gingivalis, prevotella intermedia/prevotella nigrescens, fusobacterium nucleatum, campylobacter rectus, eikenella corrodens, bacteroides forsy ... | 1997 | 9395099 |
| periodontal diseases: microbial factors. | | 1996 | 9118283 |
| reduced serum igg reactivities with bacteria from dental plaque in hiv-infected persons with periodontitis. | serum samples were obtained from 44 hiv-seropositive (hiv+) and 37 hiv-seronegative (hiv-) persons that were grouped according to periodontal status. serum igg and iga reactivities towards streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis. prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by means of elisa. hiv+ persons with chronic marginal periodontitis showed significantly lower igg reactivities to the periodontal pathoge ... | 1997 | 9402504 |
| comparison of the subgingival microbiota of periodontally healthy and diseased adults in northern cameroon. | our study is the 1st report on subgingival microbiota in adult cameronians. the aim was to investigate, using the checkerboard dna-dna hybridization technique, the prevalence of 18 oral species in subgingival plaque samples obtained from sex- and age-matched cameronian adults with and without periodontal destruction. we also compared cultivation and the affirm dp test with the checkerboard technique in their capability to detect some selected species among the 18. 21 adult periodontitis patients ... | 1997 | 9402505 |
| hen egg-white lysozyme inhibits biological activities of lipopolysaccharides from periodontopathic bacteria. | lysozyme has a bactericidal activity for some strains of gram-positive bacteria, by enzymatic cleavage of peptidoglycans that constitute the cell wall. hen egg-white lysozyme (hel) was tested in vitro for effects on biological activities of lipopolysaccharides from periodontopathic gram-negative bacteria. actinobacillus actinomycetemcomitans, prevotella intermedia and porphyromonas gingivalis. hel inhibited a wide range of activities of these lipopolysaccharides, including activation of limulus ... | 1997 | 9138195 |
| [microflora of periodontal pockets in advanced periodontitis]. | the aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. from each subject 16-20 samples were taken using paper points. pooled sample after 60 s. mixing was serially diluted in reduced bhi. for total cell counts and for the isolation of black pigmented anaerobes brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without kanamycin-vancomycin mixture and bm agar plates were used. for isolation of a. actinomycetemcomitans ... | 1997 | 9411079 |
| comparative in-vitro and in-vivo activity of am-1155 against anaerobic bacteria. | the in-vitro activity of am-1155, a 6-fluoro-8-methoxy quinolone, was compared with those of temafloxacin, sparfloxacin, tosufloxacin, ciprofloxacin, ofloxacin and cefmetazole, a cephamycin, against a variety of anaerobic bacteria. although am-1155 demonstrated only modest activity against the bacteroides fragilis group and prevotella bivia (mic90s > or =3.13 mg/ml), 76% of the b. fragilis strains tested were inhibited at am-1155 concentrations of 0.78 mg/l. am-1155 was highly active against pre ... | 1997 | 9421310 |
| clinical and microbiological effects of subgingival antimicrobial irrigation with citric acid as evaluated by an enzyme immunoassay and culture analysis. | the purpose of the present study was to compare an enzyme immunoassay with culture samples from untreated and non-surgically treated periodontal pockets and to assess the clinical and microbiological effects of citric acid irrigation as a supplement to scaling and root planing. the enzyme immunoassay used in this study is a chairside diagnostic tool aimed at identifying the presence of p. gingivalis, p. intermedia, and a. actinomycetemcomitans. six sites with pocket depths > or = 6 mm in each of ... | 1997 | 9150039 |
| periodontitis patient and the spouse: periodontal bacteria before and after treatment. | suspected periodontal pathogens can be transmitted between spouses. the treatment response may be unsuccessful in periodontitis patients, if the spouse harbors these bacteria. the aim of the present 6-month follow-up study was to clarify whether the microbiological treatment outcome of periodontitis patients is related to the detection of suspected periodontal pathogens in the saliva of the spouse. 10 patients with advanced periodontitis and their spouses were included in the study. the patients ... | 1997 | 9442426 |
| subgingival temperature: relation to gingival crevicular fluid enzymes, cytokines, and subgingival plaque micro-organisms. | there have been no reports on the relationship of subgingival temperature to specific gingival crevicular fluid (gcf) components. therefore, the purpose of this cross-sectional study was to determine whether there was any relationship between subgingival temperature and gcf levels of neutrophil elastase (ne), myeloperoxidase (mpo), beta-glucuronidase (bg), interleukin-1 alpha (il-1), and interferon alpha (ifn). furthermore, another objective was to confirm an association of subgingival temperatu ... | 1997 | 9442427 |
| oral food consumption and subgingival microorganisms: subgingival microbiota of gastrostomy tube-fed children and healthy controls. | this study examined the effect of oral food consumption on the prevalence and levels of subgingival bacteria and yeasts in 20 gastrostomy tube-fed children and 24 healthy controls. microbial identification was carried out using anaerobic culture and 16s rrna-based pcr identification methods. streptococcal and actinomyces species were recovered from 100% and 76% of all subjects and averaged 66% and 11% of total cultivable organisms, respectively. in decreasing order of prevalence, fusobacterium, ... | 1997 | 9444590 |
| prevalence of six periodontal pathogens detected by dna probe method in hiv vs non-hiv periodontitis. | the aim of the study was to examine the prevalence of selected periodontal pathogens associated with hiv and non-hiv related periodontal lesions. | 1997 | 9456680 |
| microbiology of ligature-induced marginal inflammation around osseointegrated implants and ankylosed teeth in cynomolgus monkeys (macaca fascicularis). | the microbiota associated with ligature-induced marginal inflammation around osseointegrated dental implants, ankylosed teeth, and normal control teeth was investigated in 8 cynomolgus monkeys (macaca fascicularis). submucosal/subgingival plaque was sampled with paper points on the day of literature placement and after 7 weeks. the samples were evaluated by phase-contrast microscopy and by cultivation on enriched non-selective and various solid media. the submucosal/subgingival flora was changed ... | 1996 | 9151583 |
| extracts of prevotella intermedia and actinobacillus actinomycetemcomitans inhibit alkaline phosphatase activity in osteoblastic cells in vitro. | in order to study the effects of sonicated extracts from prevotella intermedia, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and other oral-related bacteria, as well as escherichia coli on bone formation, clone mc3t3-e1 cells, which have retained osteoblastic activity, were cultured with various bacterial extracts. the addition of the sonicated extracts from prevotella intermedia and actinobacillus actinomycetemcomitans decreased the alkaline phosphatase activity in a dose-dep ... | 1997 | 9467351 |
| anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis detected by indirect immunofluorescence. | aiming to assess the presence of selected anaerobic microorganisms in root canals of human teeth with chronic apical periodontitis. 25 central and lateral upper incisors presenting with radiographic evidence of chronic apical periodontitis were studied. the pulp chamber was opened under aseptic conditions and samples of the root canal content were collected with sterile absorbent paper points, which were placed and dispersed in test tubes containing reduced transport medium rtt. aliquots were dr ... | 1996 | 9028199 |
| acid tolerance response and survival by oral bacteria. | using 21 species of oral bacteria, representing six acidogenic genera, we undertook to determine whether the ph-limiting exponential growth is related to the ability of the organisms to generate an acid-tolerance response that results in enhanced survival at low ph. the lower ph limit of exponential growth varied by more than two units with that of neisseria a182 at ph 6.34; growth of lactobacillus casei rb1014 stopped at ph 3.81, with species of actinomyces, enterococcus, prevotella and strepto ... | 1997 | 9467379 |
| tetracycline resistance in prevotella isolates from periodontally diseased patients is due to the tet(q) gene. | tetracycline-resistance in gram-negative periodontal bacteria is often due to the presence of the tet(q) gene. in the present study the polymerase chain reaction (pcr) was used to examine 54 isolates of gram-negative anaerobic rods (prevotella intermedia, prevotella nigrescens and related or bacteroides-like species) for the presence of the tet(q) gene. the isolates were recovered from 42 patients with periodontal disease living in northern europe and north america. an 814 base-pair segment of t ... | 1996 | 9028255 |
| alveolar bone loss in rats infected with a strain of prevotella intermedia and fusobacterium nucleatum isolated from a child with prepubertal periodontitis. | prevotella intermedia and fusobacterium nucleatum are associated with various forms of periodontal disease. the purpose of the present study was to infect the clinical isolates of these periodontopathic bacteria and to induce a significant loss of alveolar bone in specific pathogen-free (spf) rats in the absence of ligatures. p. intermedia ykd8 and f. nucleatum ykz5 were isolated from a prepubertal periodontitis patient, while p. gingivalis mwb13 was from a patient with juvenile periodontitis. a ... | 1997 | 9029446 |
| [the significance of endocrine factors and microorganisms in the development of gingivitis in pregnant women]. | 40-100% of pregnant women suffer from the co-called pregnancy gingivitis. the cause of pregnancy gingivitis is possible multicausal: increased plasma female sex-hormones, alteration in dental plague and perhaps prevotella intermedia in the subgingival plague, together with alteration of immunoresponse. increasing levels of progesterone in the gingiva as well as estrogens due to specific receptors affect vascular permeability and exudation, provoke stasis of microcirculation, increase prostagland ... | 1996 | 9036576 |
| the influence of abutment surface roughness on plaque accumulation and peri-implant mucositis. | bacterial adhesion to intra-oral, hard surfaces is firmly influenced by the surface roughness to these structures. previous studies showed a remarkable higher subgingival bacterial load on rough surfaces when compared to smooth sites. more recently, the additional effect of a further smoothening of intra-oral hard surfaces on clinical and microbiological parameters was examined in a short-term experiment. the results indicated that a reduction in surface roughness below r(a) = 0.2 microns, the s ... | 1996 | 9151584 |
| [bacteriological study of oral open abscesses]. | although studies of bacteriology of closed oral abscesses have been extensively done, there are few studies on microorganisms involving open oral abscesses. we examined bacteriologically three open abscesses with precaution against bacterial contamination with oral normal flora and saliva, when sampling. the specimens were subjected to aerobic and anaerobic cultures within 2 hours after sampling. all three cases were infected with 5 to 14 species of aerobic and anaerobic bacteria; streptococcus ... | 1997 | 9483884 |
| the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia using an elisa in an adolescent population with early periodontitis. | the aim of the study was to compare the occurrence and levels of a. actinomycetemcomitans, p. gingivalis, and p. intermedia in the subgingival plaque from sites with and without early periodontitis in adolescents using an elisa. 47, 15- to 16-year-old adolescents (39 indo-pakistani, 8 white caucasian) were examined for clinical attachment level, probing depth, supragingival plaque, subgingival calculus and bleeding on probing on the mesio-buccal and disto-buccal aspects of the 1st molars and the ... | 1997 | 9049799 |
| microbial complexes in subgingival plaque. | it has been recognized for some time that bacterial species exist in complexes in subgingival plaque. the purpose of the present investigation was to attempt to define such communities using data from large numbers of plaque samples and different clustering and ordination techniques. subgingival plaque samples were taken from the mesial aspect of each tooth in 185 subjects (mean age 51 +/- 16 years) with (n = 160) or without (n = 25) periodontitis. the presence and levels of 40 subgingival taxa ... | 1998 | 9495612 |
| the clinical and microbiological effects of non-surgical periodontal therapy in smokers and non-smokers. | 28 patients, 13 smokers and 15 non-smokers with untreated advanced periodontal disease, were subjected to a series of oral hygiene instructions and treated with non-surgical periodontal therapy. baseline values regarding clinical data did not differ significantly between the groups. 6 months following therapy the full-mouth bleeding score among smokers was 36.5% as compared to 22.7% for non-smokers (p < 0.05). probing depth was reduced by 1.9 mm for smokers and by 2.5 mm for non-smokers. this di ... | 1998 | 9495614 |
| the influence of periodontitis on the subgingival flora around implants in partially edentulous patients. | the hypothesis that teeth act as reservoirs of micro-organisms for the colonization of oral implants has recently been stated several times. the present study aimed at examining, in partially edentulous patients with severe periodontitis, whether pockets around teeth and implants harbored a comparable micro-flora. in 6 patients (3 with refractory periodontitis and 3 with advanced chronic adult periodontitis), plaque samples were taken from a deep and shallow pocket around both teeth and implants ... | 1996 | 9151609 |
| evaluation of three different dental implants in ligature-induced peri-implantitis in the beagle dog. part ii. histology and microbiology. | the purpose of this study was to evaluate experimental peri-implant breakdown microbiologically, radiographically and histologically. hydroxyapatite-coated, titanium plasma-sprayed, and titanium alloy surfaces were investigated. eighty-four implants were placed in 14 beagle dogs. standardized radiographs and microbiologic samples (dna) were obtained. dogs were sacrificed at 3 and 6 months. undecalcified histologic sections were prepared. thickness of hydroxyapatite coating, changes in crestal bo ... | 1998 | 9509781 |
| rapid detection of actinobacillus actinomycetemcomitans, prevotella intermedia and porphyromona gingivalis by multiplex pcr. | the identification of specific periodontal pathogens by conventional methods, mainly anaerobic cultivation, is difficult, time consuming and even sometimes unreliable. therefore, a multiplex pcr method for simultaneous detection of actinobacillus actinomycetemcomitans (a.a.), porphyromona gingivalis (p.g.) and prevotella intermedia (p.i.) was developed for rapid and easy identification of these specific bacterial pathogens in subgingival plaque samples. in this paper, there is a detailed descrip ... | 1998 | 9524322 |
| the effects of smoking on periodontal structures: a literature review. | periodontal disease seems to be more prevalent in smokers than in nonsmokers. studies have reported both increases and decreases in gingival blood flow due to smoking. smoking does not increase the presence of the periodontopathogens actinobacillus actinomycetemcomitans, porphyromonas gingivalis and bacteroides intermedius. both the chemotaxis and the phagocytic capacity of the polymorphonuclear leukocytes (pmns) harvested from smokers are lower than with those harvested from nonsmokers. further ... | 1997 | 9170751 |
| pcr-dna probe assays for identification and detection of prevotella intermedia sensu stricto and prevotella nigrescens. | the purpose of this study was to construct pcr-dna probe assays specific for prevotella intermedia sensu stricto and prevotella nigrescens based on the ability of randomly amplified polymorphic dna (rapd) fingerprinting to generate species-specific markers. the strategy included four steps: (i) construction of first-generation dna probes from a 850-bp rapd marker for p. intermedia sensu stricto and a 1,300-bp rapd marker for p. nigrescens, (ii) cloning and sequencing of each rapd marker, (iii) d ... | 1997 | 9196214 |
| 5 alpha reductase activity in human gingiva and gingival fibroblasts in response to bacterial culture supernatants, using [14c]4-androstenedione as substrate. | 5 alpha-reduction of androgen substrates is increased in inflamed gingiva. it was therefore relevant to study the effect of bacterial culture supernatants derived from prevotella intermedius (p.i), porphyromonas gingivalis (p.g) and actinobacillus actinomycetemcomitans (a.a) on the metabolism of [14c]4-androstenedione to 5 alpha-dihydrotestosterone (dht) in gingival tissue and cultured fibroblasts. chronically inflamed human gingival tissue and cultured gingival fibroblasts from the same source ... | 1997 | 9222443 |
| antimicrobial compounds from ceanothus americanus against oral pathogens. | during the search for antimicrobial compounds from higher plant sources, a methanol extract of ceanothus americanus demonstrated antimicrobial activity against selected oral pathogens. through further bioassay-guided fractionation and purification, three triterpenes (ceanothic acid, 27-hydroxy ceanothic acid and ceanothetric acid) and two flavonoids (maesopsin and maesopsin-6-o-glucoside) were identified. among these, ceanothetric acid and maesopsin-6-o-glucoside were new compounds. ceanothic ac ... | 1997 | 9276981 |
| topographic distribution of black-pigmenting anaerobes before and after periodontal treatment by local delivery of tetracycline. | the purpose of this study was to determine the distribution of black-pigmenting gram-negative bacteria in the dentition of 10 adult periodontitis patients before and after treatment by local delivery of tetracycline. the subjects were selected based on a screening for high counts of porphyromonas gingivalis and multiple deep pockets. subgingival microbial samples were taken from the mesial and distal aspect of every tooth (44 to 56 sites per patient) before treatment, and after one and two month ... | 1996 | 8915018 |
| characterization of the lactoferrin-dependent inhibition of the adhesion of actinobacillus actinomycetemcomitans, prevotella intermedia and prevotella nigrescens to fibroblasts and to a reconstituted basement membrane. | lactoferrin was previously shown to inhibit the adhesion of a. actinomycetemcomitans, p. intermedia and p. nigrescens to human cells. lactoferrin was also shown to competitively inhibit the binding of these bacteria to the basement membrane protein laminin. the present study aimed to determine the type of interactions inhibited by lactoferrin. lactoferrin binds to fibroblast monolayers and matrigel, a reconstituted basement membrane, through ionic interactions. the adhesion of a. actinomycetemco ... | 1997 | 9350211 |
| distribution and genetic analysis of oral prevotella intermedia and prevotella nigrescens. | a total of 344 prevotella intermedia and nigrescens group isolates from 59 subjects were identified by hybridization with nonradioactively labeled species-specific oligonucleotide probes. identification of 20 p. intermedia and 46 p. nigrescens isolates was confirmed by analyzing the electrophoretic mobilities of malate and glutamate dehydrogenase enzymes. a total of 111 isolates (32%) were identified as p. intermedia and 233 isolates (68%) as p. nigrescens. identification performed with oligonuc ... | 1996 | 8941760 |
| prevalence of suspected periodontal pathogens identified using elisa in adolescents of differing ethnic origins. | the aim of this study was to determine the prevalence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis and prevotella intermedia in a group of adolescents and investigate the association of these organisms with various clinical parameters. a total of 527, 11-13-year-old children, of whom 333 (63%) were white caucasian, 187 (35%) indo-pakistani and 7 (1%) afro-caribbean, participated in the study. subgingival plaque samples, collected from the mesio-buccal of both upper first per ... | 1997 | 9083896 |
| a novel component different from endotoxin extracted from prevotella intermedia atcc 25611 activates lymphoid cells from c3h/hej mice and gingival fibroblasts from humans. | a novel immunobiologically active fraction was prepared from a phenol-water extract of prevotella intermedia atcc 25611 by sephadex g-100 column chromatography. the fraction consisted mainly of carbohydrate and protein and was devoid of fatty acid. the fraction showed high-molecular-weight bands (10,000 to 12,000) on deoxycholate polyacrylamide gel electrophoresis (doc-page) and was scarcely active in a limulus test. we designated the fraction prevotella glycoprotein (pgp). the pgp fraction show ... | 1997 | 9353030 |
| characterization of prevotella intermedia and prevotella nigrescens by enzyme production, restriction endonuclease and ribosomal rna gene restriction analyses. | restriction endonuclease analysis, rrna gene restriction analysis (ribotyping), multilocus enzyme electrophoresis and lipase production were investigated for their potential to differentiate isolates belonging to the closely-related species prevotella intermedia and prevotella nigrescens. of 122 strains identified originally as p. intermedia, 52 were assigned to p. intermedia and 68 to p. nigrescens using multilocus enzyme electrophoresis. all 39 p. intermedia and 52 out of 53 p. nigrescens test ... | 1996 | 8941766 |
| killing of fusobacterium nucleatum, porphyromonas gingivalis and prevotella intermedia by protegrins. | protegrins are broad spectrum antibiotic peptides isolated from porcine leukocytes. in this study, we (i) examine the sensitivity of gram-negative, anaerobic periodontal pathogens to synthetic protegrins; (ii) determine the relative potencies of protegrin congeners against these bacteria; and (iii) compare the potency of protegrins with other antibiotic peptides, including magainin msi-78, tachyplesin i, cecropin p1, human defensins hnp-1-3, and clavanin a. synthetic l- and d-enantiomers of prot ... | 1998 | 9553868 |
| porphyromonas gingivalis, prevotella intermedia and bacteroides forsythus in plaque subjacent to bridge pontics. | this study examined the distribution of p. gingivalis, p. intermedia and b. forsythus in plaque on metallic and porcelain pontics adjacent to healthy and inflamed mucosa. subpontic plaque was collected from 33 inflamed and 31 healthy sites. plaque suspension was incubated with specific rabbit antisera to p. gingivalis (fdc 381), p. intermedia (atcc 25261) and b. forsythus (fdc 335), and the labelled cells disclosed with fluorescein-labelled goat-anti-rabbit igg by indirect immunofluorescence mic ... | 1998 | 9565285 |
| the laboratory diagnosis of periodontal infections. | | 1995 | 9567931 |
| a 5-year study of attachment loss and tooth loss in community-dwelling older adults. | tooth loss is a widely recognized endpoint measure for the effects of periodontal diseases and the impact of periodontal therapy. in fact, traditional clinical measures of periodontal status often are considered to be surrogate endpoints in that they are assumed to be related to tooth loss. however, the strength of the relationship between attachment loss and tooth loss in a representative population of untreated subjects has not been studied extensively. the purpose of this paper is to present ... | 1997 | 9379319 |
| dental plaque flora of the dog with reference to fastidious and anaerobic bacteria associated with bites. | animal bite wounds are amongst the most common types of traumatic injuries in humans. the organisms isolated from these wounds generally reflect the oral flora of the biting animal, and may be fastidious in nature and difficult to identify. this study was undertaken to determine the prevalence of eikenella corrodens, actinobacillus actinomycetemcomitans, porphyromonas and prevotella spp. in supragingival dental plaque collected from the right maxillary canine and carnassial teeth and the right m ... | 1997 | 9571899 |
| beta-sheet antibiotic peptides as potential dental therapeutics. | small, cysteine-rich, beta-sheet peptide antibiotics are found throughout the animalia. though broad spectrum in potential, they may exert selective antimicrobial effects under certain conditions. we have explored the antimicrobial properties of two families of beta-sheet peptide antibiotics, defensins and protegrins, against periodontopathic bacteria. the rabbit defensin np-1 was active against facultative gram-negative bacteria associated with early onset periodontitis, including actinobacillu ... | 1998 | 9573496 |
| identification of hemolytic activity in prevotella intermedia. | hemolysin production was measured in strains of prevotella intermedia. zones of beta-hemolysis were detected on agar plates supplemented with either sheep, rabbit or human erythrocytes. a standard tube assay was performed on cell suspensions of the organism to measure hemolytic activity, which was found to be dose dependent, eliminated by heat treatment, and saturable with increasing concentrations of blood. growth-phase experiments suggested that hemolysin production was increased during logari ... | 1998 | 9573800 |
| longitudinal evaluation of the development of periodontal destruction in spouses. | the aim of the present investigation was to study longitudinally the periodontal condition of married couples. the data are derived from a longitudinal study in a young population living in a remote village in indonesia and showing a relatively high prevalence of periodontal destruction. in 23 married couples, clinical measurements were carried out in 1987 and 1994. during the latter examination, a pooled gingival sample was obtained for microbiological evaluation. in 1994, the mean age of the g ... | 1996 | 8951630 |
| identification of bacterial species on or in crevicular epithelial cells from healthy and periodontally diseased patients using dna-dna hybridization. | the purpose of this investigation was to identify bacterial species present on or in crevicular epithelial cells in healthy and diseased sites using dna probes. in order to achieve this aim, further improvements were made in the separation of unattached bacteria from those adherent to epithelial cells isolated from the human gingival crevice or periodontal pocket. then the dna probes were used to determine the prevalence of detectable dna from 15 microbial species on or in crevicular epithelial ... | 1998 | 9573819 |
| beta-lactamase-producing strains in the species prevotella intermedia and prevotella nigrescens. | a total of 96 strains were collected that included laboratory strains and clinical isolates classified prevotella intermedia sensu lato and the type strains of the species p. intermedia sensu stricto and prevotella nigrescens. susceptibility to amoxicillin and amoxicillin-clavulanic acid was determined by the etest. pcr-dna probe assays were used to speciate each strain as p. intermedia sensu stricto or p. nigrescens. by etest, 71 strains (74%) were susceptible to both amoxicillin and amoxicilli ... | 1998 | 9573820 |
| chemiluminescent assay of alkaline phosphatase in human gingival crevicular fluid: investigations with an experimental gingivitis model and studies on the source of the enzyme within crevicular fluid. | the purpose of this study was to investigate how levels of gingival crevicular fluid (gcf) alkaline phosphatase (alp) change in relation to levels of plaque and gingival inflammation in 20 adults during a 21 day period of experimental gingivitis. the source of alp within gcf was also investigated using a repeat sampling protocol; by determining enzyme levels derived from 30 putative periodontal pathogenic and non-pathogenic species; and by examining inhibition profiles from a variety of host and ... | 1996 | 8811480 |
| clinical and microbiological effects of minocycline-loaded microcapsules in adult periodontitis. | clinical and microbiological effects of subgingival delivery of 10% minocycline-loaded (mc), bioabsorbable microcapsules were examined in 15 adult periodontitis patients. patients received oral hygiene instruction 2 weeks prior to the study. at baseline (day 0) all teeth received supragingival scaling (sc); 2 quadrants received no further treatment and 1 quadrant received subgingival scaling and root planning (srp). in the fourth quadrant, the tooth with the deepest probing sites (at least 1 sit ... | 1997 | 9407404 |
| coronal leakage of obturated root canals after long-term storage using a polymicrobial marker. | this in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker on teeth root filled with lateral condensation of cold gutta-percha and one of two sealers. sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. the teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either apexit or tubliseal ewt sealer. the teeth were stored for 6 months in artificial saliva and tested for lea ... | 1997 | 9587271 |
| occurrence of prevotella nigrescens and prevotella intermedia in infections of endodontic origin. | the occurrence of prevotella intermedia and prevotella nigrescens in endodontic infections was studied using sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page) of whole cell protein to distinguish between the species. previous studies have shown an association between black-pigmented bacteria (bpb) and endodontic infections and that prevotella intermedia (previously known as bacteroides intermedius) was the most commonly isolated bpb. recently, however, strains identified as p. ... | 1997 | 9587274 |
| effect of triclosan on the subgingival microbiota of periodontitis-susceptible subjects. | the present study evaluated the long-term effect of (i) meticulous self-performed, supragingival plaque control and (ii) the use of a triclosan/copolymer containing dentifrice in adult subjects susceptible to destructive periodontitis. 40 individuals were recruited into the trial. 3-5 years prior to the baseline examination, they had all been treated by nonsurgical means- for advanced periodontal disease. during the subsequent maintenance phase, all subjects had at different time intervals exhib ... | 1997 | 9442424 |
| the binding and utilization of hemoglobin by prevotella intermedia. | prevotella intermedia, a putative periodontopathic microorganism, requires iron for growth. hemoglobin can be a major source of iron for bacterial growth in vivo since it is present in the crevicular fluid collected from periodontitis sites. experiments studying the growth of p. intermedia in iron-depleted todd-hewitt broth supplemented with human hemoglobin showed that the bacteria were able to utilize human hemoglobin as a source of iron. the uptake of iron from hemoglobin by p. intermedia app ... | 1998 | 9627957 |
| the use of metronidazole and amoxicillin in the treatment of advanced periodontal disease. a prospective, controlled clinical trial. | the present clinical trial was performed to study the effect of systemic administration of metronidazole and amoxicillin as an adjunct to mechanical therapy in patients with advanced periodontal disease. 16 individuals, 10 female and 6 male, aged 35-58 years, with advanced periodontal disease were recruited. a baseline examination included assessment of clinical, radiographical, microbiological and histopathological characteristics of periodontal disease. the 16 patients were randomly distribute ... | 1998 | 9650870 |
| bacterial reduction in periodontal pockets through irradiation with a diode laser: a pilot study. | this study examines the application of a diode laser with a wavelength of 805 nm for periodontal treatment. while the use of the diode laser in this field has not been investigated so far, several authors have reported on the use of neodymium:yttrium-aluminum-garnet (nd:yag) laser for such applications. the aim of this study was to examine the immediate effect of the diode laser in reducing the bacterial concentration in periodontal pockets. important periodontal indices (pbi, cpitn) were assess ... | 1997 | 9467340 |
| similarity of salivary and subgingival prevotella intermedia and prevotella nigrescens isolates by arbitrarily primed polymerase chain reaction. | the distribution and the genetic similarity of prevotella intermedia and prevotella nigrescens in saliva and in subgingival samples recovered from the same subject were studied in 16 subjects with different periodontal status. the isolates (4 salivary and 4 subgingival p. intermedia/nigrescens group isolates per subject) were identified to species level by hybridization with species-specific oligonucleotide probes, and the clonal analysis was performed using arbitrarily primed polymerase chain r ... | 1996 | 9467372 |
| evidence for serum immunoglobulin g (igg) antibody responses in macaca fascicularis identified by monoclonal antibodies to human igg subclasses. | this investigation determined the capacity of murine monoclonal antibodies directed to human immunoglobulin g (igg) subclasses to identify molecules with conserved epitopes in the serum of the nonhuman primate, macaca fascicularis. we subsequently utilized this cross-reactivity to document the characteristics of igg subclass antibody responses in m. fascicularis to parenteral immunization with intact oral microorganisms, antigens from oral microorganisms, and finally a defined protein toxin, tet ... | 1997 | 9467387 |
| characteristics of systemic antibody responses of nonhuman primates to cell envelope and cell wall antigens from periodontal pathogens. | the immune response of the primate, macaca fascicularis, to cell envelope (cea) or cell wall (cwa) antigens of several periodontal pathogens was examined to develop a strategy to interfere with ligature-induced periodontitis. animals were parenterally immunized with cea of either porphyromonas gingivalis, prevotella intermedia or a combination of cea/cwa of campylobacter rectus, fusobacterium nucleatum and actinomyces viscosus. serum samples were taken every 2-4 weeks over a 4-month period, whic ... | 1997 | 9467388 |
| evaluation of the antimicrobial effect of lactoferrin on porphyromonas gingivalis, prevotella intermedia and prevotella nigrescens. | the antimicrobial effect of lactoferrin (apolf) on the oral, black-pigmented anaerobes porphyromonas gingivalis, prevotella intermedia and p. nitrescens has been studied. apolf did not kill any of these species but it did inhibit the growth of p. gingivalis, while iron-saturated lf (felf) had no effect. the other two species were unaffected by apolf. this growth inhibitory effect of apolf could not be explained on the basis of chelation of inorganic iron, since growth of p. gingivalis occurred i ... | 1998 | 9657318 |
| pathogenicity of prevotella intermedia and prevotella nigrescens isolates in a wound chamber model in rabbits. | the pathogenicity of 14 isolates identified as prevotella intermedia or prevotella nigrescens by serogrouping using monoclonal antibodies was compared in a tissue cage model in rabbits. seven strains from periodontal abscesses, 5 strains from deep periodontal pockets and 2 strains from gingivitis were tested in the animal model comprising 6 teflon tissue cages implanted on the back each of 34 rabbits. a total of 10(5)-10(8) cells of p. intermedia or p. nigrescens strains were inoculated alone or ... | 1997 | 9467400 |
| the efficacy of antimicrobial mouth rinses in oral health care. | there is growing public recognition of the importance of oral health, as symbolized by the theme. "oral health for a healthy life" proposed for the 1994 world health day. in this report, the efficacy of antimicrobial mouth rinses, mainly listerine, was reviewed by three investigators who are working as a microbiologist, a microbiologist, a dentist, and a dental hygienist participating in oral health care. listerine, an antimicrobial mouth rinse, completely killed microorganisms in 10 to 30 secon ... | 1998 | 9663026 |
| salivary iga responses to bacteria in dental plaque as related to periodontal and hiv infection status. | levels of total iga and specific iga reactive with streptococcus mutans, actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens and fusobacterium nucleatum were measured by elisa in parotid saliva from hiv+ and hiv- persons with healthy gingiva (hg), chronic gingivitis, chronic marginal periodontitis (cmp), or necrotizing ulcerative periodontitis (nup). when the hiv+ group was compared with the hiv- group regardless of periodontal status, tot ... | 1997 | 9469606 |
| local and systemic tcr v gene expression in advanced periodontal disease. | the aim of the present investigation was to study the expression of specific alpha/beta t cell receptor (tcr) gene products in relation to some microbiological and immunological features of advanced destructive periodontitis. 21 individuals with advanced periodontal disease (diseased group) and 16 age- and sex-matched healthy subjects (healthy group) were recruited. following a clinical examination of the diseased group, the 3 deepest interproximal sites in the upper and lower premolar- or molar ... | 1998 | 9495611 |
| the role of local factors in the etiology of periodontal diseases. | | 1993 | 9673183 |
| palmar plantar keratosis and unusual periodontal findings. observations from a family of 4 members. | the connection between palmar plantar hyperkeratosis and severe periodontal disease was first reported in 1924 by papillon and lefevre. the 2 major components of this syndrome (pls) can also occur as distinct entities. the literature describes a number of cases which do not fit the classical disease descriptions. in this paper, we report on a family with an atypical pls. the father had marked palmar plantar hyperkeratosis but a very late onset of destructive marginal periodontitis. the son also ... | 1998 | 9495618 |
| periodontal status and subgingival microbiota of insulin-dependent juvenile diabetics: a 3-year longitudinal study. | this study examined for 3 years the changes in periodontal status and the possible correlations with selected subgingival microbiota and diabetic conditions in a group of 16 insulin-dependent diabetes mellitus (iddm, jd) patients as compared with their 16 healthy cohabiting siblings (hs). jd patients were monitored every 3 months for levels of glycosylated hemoglobin (hba1c). clinical and microbiological parameters were measured 6 weeks before drawing blood to determine levels of hba1c. periodon ... | 1998 | 9526910 |
| microbiota associated with experimental peri-implantitis and periodontitis in adult macaca mulatta monkeys. | this study examines the microbiota associated with the progression of experimental peri-implantitis and periodontitis induced concurrently in partially edentulous adult monkeys. root-form and plate-form implants with fixed prosthesis in place for at least 12 months and their corresponding opposite molar teeth were ligated for 6 months. the microbiota in plaque around these ligated dental implants and molars were studied at 0, 1, 2, 3, and 6 months post-ligation. plaque samples were analyzed by d ... | 1998 | 9526919 |
| the effect of supragingival plaque control on the progression of advanced periodontal disease. | the aim of the present trial was to study the effect of meticulous supragingival plaque control on (i) the subgingival microbiota, and (ii) the rate of progression of attachment loss in subjects with advanced periodontal disease. an intra-individual group of sites exposed to non-surgical periodontal therapy served as controls. 12 patients with advanced periodontal disease were subjected to a baseline examination (bl) including assessments of oral hygiene status, gingival condition (bop), probing ... | 1998 | 9696252 |
| histopathological investigation of gingival tissue from patients with rapidly progressive periodontitis. | in this study, fine structural features of the pocket walls in rapidly progressive periodontitis (rpp) and adult periodontitis (ap) in 20 cases were compared using light and transmission electron microscopy. gingiva was also obtained from a control group of periodontally healthy teeth. clinical parameters were assessed in both rpp and ap patients and in controls. bone destruction and attachment loss were more marked in rpp than in ap. light microscopical observations of inflamed rpp tissue as co ... | 1998 | 9526920 |
| subgingival distribution of periodontopathic bacteria in adult periodontitis and their susceptibility to minocycline-hcl. | the purpose of this study was to investigate the distribution of several periodontopathic bacteria in adult periodontitis, their in vitro susceptibility to minocycline-hcl, and whether the efficacy of the drug changes with a decrease in bacterial susceptibility. twenty-one patients (43 to 75 years old) with 62 periodontal lesions from pockets > or =4 mm participated in the study. after subgingival sampling, an ointment containing 2% minocycline-hcl was applied locally to the selected pockets onc ... | 1998 | 9527568 |
| effects of topical metronidazole and tetracycline in treatment of adult periodontitis. | the present study was performed to assess and compare the clinical healing and the microbiological findings following local application of metronidazole or tetracycline to augment subgingival scaling in previously untreated adult periodontitis sites. eighteen patients with moderate to severe adult periodontitis at single-rooted teeth were selected. in each patient, 3 interproximal sites having comparable root anatomy, probing depth > or =5 mm and bleeding on probing were randomly assigned to 1 o ... | 1998 | 9706861 |
| the utility of whole saliva to detect the oral presence of periodontopathic bacteria. | this study compared the presence of 6 periodontopathic bacteria in whole saliva and subgingival plaque of 202 subjects. the test bacteria were identified using a 16s rrna-based pcr detection method. each study subject contributed a whole saliva sample and a paper point sample pooled from the deepest periodontal pocket in each quadrant of the dentition. the kappa test revealed a fair agreement between the presence of porphyromonas gingivalis, prevotella intermedia, and treponema denticola in whol ... | 1998 | 9706862 |
| modification of cystatin c activity by bacterial proteinases and neutrophil elastase in periodontitis. | to study the interaction between the human cysteine proteinase inhibitor, cystatin c, and proteinases of periodontitis associated bacteria. | 1997 | 9536278 |
| subgingival microbial profile of papillon-lefèvre patients assessed by dna-probes. | the prevalence of 18 selected bacterial species was assessed by means of "checkerboard" dna-dna hybridisation in a group of 12 saudi-arabian adolescents with papillon-lefèvre syndrome. a total of 36 tooth sites were investigated. the patients exhibited severe periodontal disease with deep pockets. all 12 patients harboured the putative bacterial pathogens p. intermedia, f. nucleatum, p. micros and s. intermedius while t. denticola, b. forsythus, p. nigrescens, e. corrodens, s. noxia and c. rectu ... | 1998 | 9722266 |
| crevicular fluid level of beta-glucuronidase in relation to clinical periodontal parameters and putative periodontal pathogens in early-onset periodontitis. | analysis of beta-glucuronidase (betag) in the gingival crevicular fluid (gcf) provides an indication of neutrophil influx into the crevicular environment. the aim of this study was to test the hypotheses that: (1) betag is significantly elevated in individuals with early-onset periodontitis (eop) and that betag activity correlates with disease severity; and (2) betag level may reflect the local bacterial challenge in the gingival crevice. the study subjects consisted of a sub-sample of individua ... | 1998 | 9722267 |
| oral microbiota in smokers and non-smokers in natural and experimentally-induced gingivitis. | the present study primarily aimed at investigating the oral microbiota in smokers and non-smokers with established gingivitis and monitoring its composition during experimental gingivitis. secondly, it aimed at examining whether the composition of the microbiota is associated with different levels of gingival inflammation during this experimental gingivitis trial. for this purpose, 25 non-dental university students with gingivitis were recruited. 11 subjects were smokers and 14 were non-smokers. ... | 1998 | 9722273 |
| early-onset periodontitis associated with down's syndrome--clinical interventional study. | individuals with down's syndrome (ds) have an increased prevalence of periodontal disease compared with otherwise normal, age-matched control groups and other mentally handicapped patients of similar age distribution. the exaggerated immune-inflammatory response of the tissues cannot be explained by poor oral hygiene alone and might be the result of an impaired cell-mediated and humoral immunity and a deficient phagocytic system. as far as the progression and severity of destruction, the oral ma ... | 1998 | 9722720 |
| current understanding of the role of microscopic monitoring, baking soda, and hydrogen peroxide in the treatment of periodontal disease. committee on research, science and therapy. the american academy of periodontology. | the keyes technique came to national attention following a reference in the new york times in the late 1970s. several lay press articles and discussions on national television served to further focus the interest of patients, general dentists, and periodontists on this potential approach to periodontal therapy. early evaluations of the data on the technique resulted in an academy position paper in 1981. recognizing that there was a lack of well-controlled studies on the technique led to extensiv ... | 1998 | 9736380 |
| identification and analyses of periodontal pathogens in taiwan by microbiological tests. | the purpose of this study was to use microbiological tests for diagnosis of periodontal diseases in taiwan. anaerobic culture, direct microscopy, indirect immunofluorescence (if), and biochemical tests were used to examine 336 samples for the specific microorganisms in subgingival plaque. the results indicated that gram-negative species and motile bacteria were less frequently detected, and in lower proportion, in samples from healthy sites. the bacteria found frequently in healthy group were th ... | 1994 | 9747334 |
| biochemical and genetic characterization of a prevotella intermedia/nigrescens-like organism. | thirty-three previously non-typable faintly pigmented gram-negative anaerobic bacterial isolates, biochemically most closely related to prevotella intermedia and prevotella nigrescens, were analysed for enzymic reactions, cellular fatty acid (cfa) composition, electrophoretic mobility of malate and glutamate dehydrogenases, hybridization with p. intermedia and p. nigrescens species-specific oligonucleotide probes and, for genetic heterogeneity, by arbitrarily primed pcr (ap-pcr). p. intermedia a ... | 1998 | 9542074 |
| phylogenetic characterization and proposal of a new pigmented species to the genus prevotella: prevotella pallens sp. nov. | complete 16s rrna gene sequences of three representative strains of anaerobic, gram-negative, pigmented, moderately saccharolytic, indole-positive bacteria isolated from the oral cavity of humans were determined. according to comparative analyses of the rrna sequence data, this organism represents a previously unknown species within the genus prevotella. in addition, 22 representative strains and 21 reference strains (including 11 prevotella intermedia and 10 prevotella nigrescens strains) were ... | 1998 | 9542075 |
| influence of smoking on the outcome of periodontal surgery. a 5-year follow-up. | the 5-year outcome following periodontal surgery was evaluated in 57 patients that had received regular maintenance care throughout the follow-up period. the study population included 20 smokers, 20 former smokers and 17 non-smokers in the age range 37-77 years. the clinical characteristics evaluated were supragingival plaque, gingival bleeding and pocket probing depth. the region assigned for surgery was, in addition, radiographically evaluated in terms of periodontal bone height. furthermore, ... | 1998 | 9543189 |
| untreated periodontal disease in indonesian adolescents. clinical and microbiological baseline data. | at present, several risk factors for the initiation and progression of periodontitis have been identified. in order to investigate the rôle of various potential clinical and microbiological risk factors and indicators, a longitudinal study was initiated in a young population deprived from regular dental care. the present communication describes the baseline cross-sectional data obtained in 1987 in terms of the clinical periodontal condition and prevalence of periodontal bacteria in the oral cavi ... | 1998 | 9543192 |
| coronal leakage in teeth root-filled with gutta-percha and two different sealers after long-term storage. | this in vitro study investigated the effect of long-term storage on the coronal leakage of a microbial marker in teeth root-filled with lateral condensation of cold gutta-percha and one of two sealers. sixty single-rooted teeth were prepared chemomechanically to a size 40 master apical file. the teeth were divided into two groups of 20 teeth each and obturated with gutta-percha using either apexit or tubliseal ewt sealer. the teeth were stored for 6 months in artificial saliva and tested for lea ... | 1997 | 9550035 |
| purification and functional analysis of the dnak homologue from prevotella intermedia omz 326. | this study examined heat shock proteins (hsps) of the periodontal pathogen prevotella intermedia and the closely related species, prevotella nigrescens and prevotella corporis. after heat shock at 45 degrees c for 5 min, cell-free extracts were analysed by sds-page and western blotting with polyclonal antibodies against escherichia coli hsps. p. intermedia, p. nigrescens and p. corporis all expressed a dnak homologue. the p. nigrescens dnak was of a similar molecular mass to e. coli dnak (70 kda ... | 1998 | 9785453 |
| clinical expression of tnf-alpha in smoking-associated periodontal disease. | the level of tnf-alpha in gingival crevicular fluid (gcf) was analyzed with respect to smoking in patients with untreated moderate to severe periodontal disease including 30 current smokers, 19 former smokers and 29 non-smokers, in the age range 31-79 years. concomitantly the occurrence of the periopathogens actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg) and prevotella intermedia (pi) and the gcf levels of albumin, iga and igg were analyzed. with regard to clinical char ... | 1998 | 9797047 |
| antimicrobial susceptibilities of porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens spp. isolated in spain. | the susceptibilities of 143 porphyromonas gingivalis, prevotella intermedia, and prevotella nigrescens isolates to 18 antimicrobial agents were tested. all p. gingivalis isolates were susceptible. in contrast, some prevotella spp. (17%) were resistant to beta-lactams, erythromycin, clindamycin, or tetracycline and carried resistance genes, ermf or tetq, or beta-lactamases. | 1998 | 9797247 |
| the relation of microbiologic data to aspartate aminotransferase enzyme activity in gingival crevicular fluid. | gingival crevicular fluid (gcf), reflects the immune and inflammatory reactions and is itself a location for specific host-microbe interactions that lead to periodontal diseases. aspartate aminotransferase (ast) is one of the components of gcf that is released as a result of cell death. in this study, 40 periodontal sites in 10 early onset periodontitis patients before and after nonsurgical periodontal therapy, with and without local metronidazole administration, were first examined for the ast ... | 1996 | 9569804 |
| risk indicators for harboring periodontal pathogens. | the risk for harboring 6 putative periodontal pathogens in 4 selected periodontal pockets, in whole saliva, or in either site (i.e., orally) was determined in 52 caucasians, 49 african-americans, 48 asian-americans, and 50 hispanics living in los angeles. 16s rrna pcr analysis assessed the presence of actinobacillus actinomycetemcomitans, bacteroides forsythus, porphyromonas gingivalis, prevotella intermedia, prevotella nigrescens, and treponema denticola. step-wise regression analysis determine ... | 1998 | 9802709 |
| local metronidazole application in maintenance patients. clinical and microbiological evaluation. | the purpose of this investigation was to evaluate the clinical and microbiological effect of local antibiotic therapy in comparison with subgingival scaling and root planing in a randomized semi-masked study. forty-six recall patients who completed systematic periodontal therapy 6 to 24 months prior to the study were enrolled. the inclusion requirements were at least one site with probing depth > or = 5 mm in each quadrant, no scaling, and no antibiotic therapy during the last 6 months. after ra ... | 1998 | 9802715 |
| amoxicillin and clavulanic acid concentrations in gingival crevicular fluid. | the beta-lactams are bactericidal antibiotics, but some of them may be inactivated by bacterial beta-lactamases which destroy the beta-lactam ring. the inactivation of amoxicillin by beta-lactamases of gram negative anaerobic bacteria can be circumvented by the addition of clavulanic acid, a beta-lactamases inhibitor. thus, most of these bacteria are susceptible to this combination. the aim of this study was to investigate the concentrations of amoxicillin and clavulanic acid in gingival crevicu ... | 1997 | 9402501 |
| recovery of anaerobic bacteria from a post-traumatic nasal septal abscess. a report of two cases. | this report presents 2 children (boys, 11 and 9 years old) who had post-traumatic nasal septal abscesses. cultures for aerobic and anaerobic bacteria revealed peptostreptococcuus magnus and a beta-lactamase-producing prevotella intermedia in the first patient, and peptostreptococcus anaerobius, streptococcus intermedius, and prevotella melaninogenica in the second patient. anaerobic bacteria may play a role in nasal septal abscesses. | 1998 | 9823846 |