| acute toxicity of chemically and mechanically dispersed crude oil to juvenile sea bass (dicentrarchus labrax): absence of synergistic effects between oil and dispersants. | the goal of the present experiment was to assess the relative acute toxicities of mechanically and chemically dispersed oil (crude arabian light) in controlled conditions. juvenile sea bass (dicentrarchus labrax) were exposed to 4 commercial formulations of dispersants (corexit ec9500a, dasic slickgone ns, finasol osr 52, inipol ip 90), to mechanically dispersed oil, and to the corresponding chemical dispersions. acute toxicity was evaluated at 24 h, 48 h, 72 h, and 96 h through the determinatio ... | 2015 | 25677812 |
| estrogen-induced yolk precursors in european sea bass, dicentrarchus labrax: status and perspectives on multiplicity and functioning of vitellogenins. | the estrogen-inducible egg yolk precursor, vitellogenin, of the european sea bass (dicentrarchus labrax) has received considerable scientific attention by virtue of its central importance in determination of oocyte growth and egg quality in this important aquaculture species. however, the multiplicity of vitellogenins in the sea bass has only recently been examined. recent cloning and homology analyses have revealed that the sea bass possesses the three forms of vitellogenin, vtgaa, vtgab and vt ... | 2015 | 25637672 |
| interactive effect of high environmental ammonia and nutritional status on ecophysiological performance of european sea bass (dicentrarchus labrax) acclimated to reduced seawater salinities. | we investigated the interactive effect of ammonia toxicity, salinity challenge and nutritional status on the ecophysiological performance of european sea bass (dicentrarchus labrax). fish were progressively acclimated to normal seawater (32ppt), to brackish water (20ppt and 10ppt) and to hyposaline water (2.5ppt). following acclimation to different salinities for two weeks, fish were exposed to high environmental ammonia (hea, 20mg/l ∼1.18mm representing 50% of 96h lc50 value for ammonia) for 12 ... | 2015 | 25625520 |
| differentiation between fresh and frozen-thawed sea bass (dicentrarchus labrax) fillets using two-dimensional gel electrophoresis. | this study aimed to identify a protein marker that can differentiate between fresh skinless and frozen-thawed sea bass (dicentrarchus labrax) fillets using the two-dimensional polyacrylamide gel electrophoresis (2-de) technique. distinct gel patterns, due to proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from frozen-thawed ones. frozen-thawed fillets showed two specific protein spots as early as the first day of the study. however, these spots were not ... | 2015 | 25624236 |
| the forebrain-midbrain acts as functional endocrine signaling pathway of kiss2/gnrh1 system controlling the gonadotroph activity in the teleost fish european sea bass (dicentrarchus labrax). | some teleost species, including european sea bass, harbor two different kisspeptin coding genes: kiss1 and kiss2. both genes are expressed in the brain, but their differential roles in the central control of fish reproduction are only beginning to be elucidated. in this study, we have examined the effects of intracerebroventricular injections of the highly active sea bass peptides kiss1-15 and kiss2-12 on spermiating male sea bass. physiological saline, kiss1-15, or kiss2-12 was injected into th ... | 2015 | 25609835 |
| european sea bass genome and its variation provide insights into adaptation to euryhalinity and speciation. | the european sea bass (dicentrarchus labrax) is a temperate-zone euryhaline teleost of prime importance for aquaculture and fisheries. this species is subdivided into two naturally hybridizing lineages, one inhabiting the north-eastern atlantic ocean and the other the mediterranean and black seas. here we provide a high-quality chromosome-scale assembly of its genome that shows a high degree of synteny with the more highly derived teleosts. we find expansions of gene families specifically associ ... | 2014 | 25534655 |
| exposure to chronic moderate hypoxia impacts physiological and developmental traits of european sea bass (dicentrarchus labrax) larvae. | since european sea bass (dicentrarchus labrax) larvae occurred in coastal and estuarine waters at early life stages, they are likely to be exposed to reduced dissolved oxygen waters at a sensitive developmental stage. however, the effects of hypoxia at larval stage, which depend in part on fish species, remain very poorly documented in european sea bass. in the present study, the impacts of an experimental exposure to a chronic moderate hypoxia (40 % air saturation) between 30 and 38 days post-h ... | 2015 | 25487612 |
| hypo-osmotic stress-induced physiological and ion-osmoregulatory responses in european sea bass (dicentrarchus labrax) are modulated differentially by nutritional status. | we investigated the impact of nutritional status on the physiological, metabolic and ion-osmoregulatory performance of european sea bass (dicentrarchus labrax) when acclimated to seawater (32 ppt), brackish water (20 and 10 ppt) and hyposaline water (2.5 ppt) for 2 weeks. following acclimation to different salinities, fish were either fed or fasted (unfed for 14 days). plasma osmolality, [na(+)], [cl(-)] and muscle water content were severely altered in fasted fish acclimated to 10 and 2.5 ppt i ... | 2015 | 25483239 |
| mhc ii-β chain gene expression studies define the regional organization of the thymus in the developing bony fish dicentrarchus labrax (l.). | mhc ii-β chain gene transcripts were quantified by real-time pcr and localised by in situ hybridization in the developing thymus of the teleost dicentrarchus labrax, regarding the specialization of the thymic compartments. mhc ii-β expression significantly rose when the first lymphoid colonization of the thymus occurred, thereafter increased further when the organ progressively developed cortex and medulla regions. the evolving patterns of mhc ii-β expression provided anatomical insights into so ... | 2015 | 25475077 |
| influence of titanium dioxide nanoparticles on 2,3,7,8-tetrachlorodibenzo-p-dioxin bioconcentration and toxicity in the marine fish european sea bass (dicentrarchus labrax). | the present study investigated the influence of nano-tio(2) (1 mg l(-1)) on 2,3,7,8-tetrachlorodibenzo-p-dioxin(2,3,7,8-tcdd) (46 pg l(-1)) bioconcentration and toxicity in the european sea bass (dicentrarchus labrax) during 7 days in vivo exposure. a multimarkers approach was applied in different organs: detoxification in liver; innate immunity and pro-inflammatory response and adaptive immunity in gills and spleen; genotoxicity in peripheral erythrocytes and muscle. bioconcentration of 2,3,7,8 ... | 2015 | 25463713 |
| ecotoxicity of diethylene glycol and risk assessment for marine environment. | diethylene glycol (deg) is a chemical compound used during offshore oil activities to prevent hydrate formation, and it may be released into the sea. a full ecotoxicological characterization is required according to european and italian regulations for chemical substances. we have evaluated long-term toxic effects of deg on indicator species of the marine environment as algae (phaeodactylum tricornutum), crustaceans (artemia franciscana), molluscs (tapes philippinarum) and fish (dicentrarchus la ... | 2015 | 25463226 |
| a multidisciplinary study of the extracutaneous pigment system of european sea bass (dicentrarchus labrax l.). a possible relationship between kidney disease and dopa oxidase activity level. | infectious diseases and breeding conditions can influence fish health status. furthermore it is well known that human and animal health are strongly correlated. in lower vertebrates melano-macrophage centres, clusters of pigment-containing cells forming the extracutaneous pigment system, are widespread in the stroma of the haemopoietic tissue, mainly in kidney and spleen. in fishes, melano-macrophage centres play an important role in the immune response against antigenic stimulants and pathogens ... | 2015 | 25449383 |
| effects of dietary concentrated mannan oligosaccharides supplementation on growth, gut mucosal immune system and liver lipid metabolism of european sea bass (dicentrarchus labrax) juveniles. | the study assesses the effects of dietary concentrated mannan oligosaccharides (cmos) on fish performance, biochemical composition, tissue fatty acid profiles, liver and posterior gut morphology and gen expression of selected parameters involved on the intestinal immune response and liver lipid metabolism of european sea bass (dicentrarchus labrax). for that purpose, specimens of 20 g were fed during 8 weeks at 0 and 1.6 g kg(-1) dietary cmos of inclusion in a commercial sea bass diet. dietary c ... | 2015 | 25447638 |
| genetic inactivation of european sea bass (dicentrarchus labrax l.) eggs using uv-irradiation: observations and perspectives. | androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. it has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), x-ray or uv irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. the conditions for the genetic inactivation of the ... | 2014 | 25329931 |
| in situ mortality experiments with juvenile sea bass (dicentrarchus labrax) in relation to impulsive sound levels caused by pile driving of windmill foundations. | impact assessments of offshore wind farm installations and operations on the marine fauna are performed in many countries. yet, only limited quantitative data on the physiological impact of impulsive sounds on (juvenile) fishes during pile driving of offshore wind farm foundations are available. our current knowledge on fish injury and mortality due to pile driving is mainly based on laboratory experiments, in which high-intensity pile driving sounds are generated inside acoustic chambers. to va ... | 2014 | 25275508 |
| behavioural stress responses predict environmental perception in european sea bass (dicentrarchus labrax). | individual variation in the response to environmental challenges depends partly on innate reaction norms, partly on experience-based cognitive/emotional evaluations that individuals make of the situation. the goal of this study was to investigate whether pre-existing differences in behaviour predict the outcome of such assessment of environmental cues, using a conditioned place preference/avoidance (cpp/cpa) paradigm. a comparative vertebrate model (european sea bass, dicentrarchus labrax) was u ... | 2014 | 25264870 |
| physiological mechanisms underlying individual variation in tolerance of food deprivation in juvenile european sea bass, dicentrarchus labrax. | although food deprivation is a major ecological pressure in fishes, there is wide individual variation in tolerance of fasting, whose mechanistic bases are poorly understood. two thousand individually tagged juvenile european sea bass were submitted to two 'fasting/feeding' cycles each comprising 3 weeks of food deprivation followed by 3 weeks of ad libitum feeding at 25°c. rates of mass loss during the two fasting periods were averaged for each individual to calculate a population mean. extreme ... | 2014 | 25232198 |
| daily and seasonal expression of clock genes in the pituitary of the european sea bass (dicentrarchus labrax). | the expression of select clock genes (clock, bmal, per1, per2, cry1, cry2) was investigated throughout the day and across the four seasons for two consecutive years in the pituitary of adult sea bass (dicentrarchus labrax). a rhythmic pattern of daily expression was consistently observed in summer and autumn, while arrhythmicity was observed for some clock genes during spring and winter, concomitant with low water temperatures. the expression of clock and bmal showed highest values at the end of ... | 2014 | 25148807 |
| t cell transcripts and t cell activities in the gills of the teleost fish sea bass (dicentrarchus labrax). | the gills of fish are a mucosal tissue that contains t cells involved in the recognition of non-self and pathogens, and in this work we describe some features of gill-associated t cells of european sea bass, a marine model species. a whole transcriptome was obtained by deep sequencing of rna from unstimulated gills that has been analyzed for the presence of t cell-related transcripts. of the putative expressed sequences identified in the transcriptome, around 30 were related to main functions re ... | 2014 | 25109574 |
| trace metals in tissues of the six most common fish species in the black sea, turkey. | concentrations of mn, fe, co, ni, as, se, cd, ag and pb in scale, skin, muscle, gills, liver and the gonads of gilthead seabream (sparus aurata), european seabass (dicentrarchus labrax), black sea salmon (salmo trutta labrax), horse mackerel (trachurus trachurus), red mullet (mullus barbatus) and whiting (merlangius merlangius euxinus) from the black sea, in turkey, were investigated. elemental analyses were performed with inductively coupled plasma-mass spectrometry after sample preparation by ... | 2015 | 25082436 |
| induction of cyp1a and abc transporters in european sea bass (dicentrarchus labrax) upon 2,3,7,8-tcdd waterborne exposure. | the aim of this study was to characterize the responsiveness of cyp1a and abc transport proteins in european sea bass (dicentrarchus labrax) waterborne exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-tcdd) (46 pg/l) for 24 h and 7 days. genes modulation (abcb1, abcc1-2, cyp1a), erod activity were investigated in liver and 2,3,7,8-tcdd bioconcentration in liver and muscle. tcdd induced significantly cyp1a gene expression and erod activity at 24 h and 7 d. a significant up-regulation of ab ... | 2014 | 25016329 |
| novel galanin receptors in teleost fish: identification, expression and regulation by sex steroids. | in fish, the onset of puberty, the transition from juvenile to sexually reproductive adult animals, is triggered by the activation of pituitary gonadotropin secretion and its timing is influenced by external and internal factors that include the growth/adiposity status of the animal. kisspeptins have been implicated in the activation of puberty but peripheral signals coming from the immature gonad or associated to the metabolic/nutritional status are also thought to be involved. therefore we hyp ... | 2014 | 25016048 |
| advances in european sea bass genomics and future perspectives. | only recently available sequenced and annotated teleost fish genomes were restricted to a few model species, none of which were for aquaculture. the application of marker assisted selection for improved production traits had been largely restricted to the salmon industry and genetic and quantitative trait loci (qtl) maps were available for only a few species. with the advent of next generation sequencing the landscape is rapidly changing and today the genomes of several aquaculture species have ... | 2014 | 25011579 |
| ontogenesis of the hpi axis and molecular regulation of the cortisol stress response during early development in dicentrarchus labrax. | the cortisol stress response and the molecular programming of the corticoid axis were characterized for the first time during early ontogeny in a mediterranean marine teleost, the european sea bass (dicentrarchus labrax). sea bass embryos, pre-larvae and larvae at specific points of development were exposed to acute stressors and the temporal patterns of cortisol whole body concentrations and the expression of genes involved in corticosteroid biosynthesis, degradation and signaling were determin ... | 2014 | 24984570 |
| fish genomes provide novel insights into the evolution of vertebrate secretin receptors and their ligand. | the secretin receptor (sctr) is a member of class 2 subfamily b1 gpcrs and part of the pac1/vpac receptor subfamily. this receptor has long been known in mammals but has only recently been identified in other vertebrates including teleosts, from which it was previously considered to be absent. the ligand for sctr in mammals is secretin (sct), an important gastrointestinal peptide, which in teleosts has not yet been isolated, or the gene identified. this study revises the evolutionary model previ ... | 2014 | 24906176 |
| detecting the specific parameters that affect the maturation of farmed sea bass (dicentrarchus labrax) fillets stored in sunflower oil. | in this study, it was aimed to detect the specific parameters that effect the maturation of farmed sea bass fillets stored in sunflower oil. sea bass fillets were taken into the pickling solution (2.5% acetic acid and 11% sodium chloride) at 4 °c(±1). fish meat in each group was analysed for the following parameters; ph, moisture%, acetic acid% and nacl% in the maturation pickling solution and in sunflower oil. at the end of the 90 days storage, there were not any negative situations about the f ... | 2014 | 24876656 |
| mercury accumulation and tissue-specific antioxidant efficiency in the wild european sea bass (dicentrarchus labrax) with emphasis on seasonality. | the main goal of this study was to assess both mercury (hg) accumulation and organs' specific oxidative stress responses of gills, liver and kidney of dicentrarchus labrax with emphasis on seasonality. fish were collected in cold and warm periods in three stations: reference, moderated and highly contaminated sites. our results showed that seasonal factors slightly influenced hg accumulation between year periods (cold and warm) and strongly affected organs' response basal levels. in contrast, se ... | 2014 | 24875309 |
| seasonal variations of the humoral immune parameters of european sea bass (dicentrarchus labrax l.). | seasonal cycles, mainly due to great variations in the light duration and temperature, are important and modulate several aspects of the animal behavior. in the case of poikilotherms animals such as fish this is very relevant. thus, temperature changes fish immunity and affects disease resistance. we evaluate in this work the season variations of the european sea bass (dicentrarchus labrax) humoral innate parameters focusing on winter months, at which the culture of this specie is more difficult ... | 2014 | 24852342 |
| fourier transform infrared spectroscopy as a tool to study farmed and wild sea bass lipid composition. | the lipids of 16 farmed and wild european sea bass (dicentrarchus labrax) samples were studied by fourier transform infrared (ftir) spectroscopy. the spectroscopic parameters which would be useful when distinguishing between both fish origins were analysed. | 2014 | 24834498 |
| variations in toxic metal levels of two fish species, pomatomus saltatrix and dicentrarchus labrax, and risk estimation for children. | the concentrations of five toxic metals were monthly determined in two fish species, obtained from fish markets in turkey during 2010-2011. for the determinations, aas and icp-aes were used. the obtained lead concentrations for all studied pomatomus saltatrix (mean 635 microg x kg(-1)) and dicentrarchus labrax (mean 463 microg x kg(-1)) samples were found to be significantly higher than the maximum allowances concentration (mac) of 300 microg x kg(-1). mean chromium (324 microg x kg(-1)) and cu ... | 2014 | 24822389 |
| generation and characterization of the sea bass dicentrarchus labrax brain and liver transcriptomes. | the sea bass dicentrarchus labrax is the center of interest of an increasing number of basic or applied research investigations, even though few genomic or transcriptomic data is available. current public data only represent a very partial view of its transcriptome. to fill this need, we characterized brain and liver transcriptomes in a generalist manner that would benefit the entire scientific community. we also tackled some bioinformatics questions, related to the effect of rna fragment size o ... | 2014 | 24768179 |
| expressional regulation of key hepatic enzymes of intermediary metabolism in european seabass (dicentrarchus labrax) during food deprivation and refeeding. | we hypothesized that the analysis of mrna level and activity of key enzymes in amino acid and carbohydrate metabolism in a feeding/fasting/refeeding setting could improve our understanding of how a carnivorous fish, like the european seabass (dicentrarchus labrax), responds to changes in dietary intake at the hepatic level. to this end cdna fragments encoding genes for cytosolic and mitochondrial alanine aminotransferase (calt; malt), pyruvate kinase (pk), glucose 6-phosphate dehydrogenase (g6pd ... | 2014 | 24746983 |
| pyrosequencing survey of intestinal microbiota diversity in cultured sea bass (dicentrarchus labrax) fed functional diets. | the routine use of chemotherapy to control bacterial diseases in aquatic populations has resulted in the development and spread of antibiotic resistance. the inclusion of immunostimulants in fish diets (functional diets) is one of the main strategies to solve this threat. this study aimed to analyse the intestinal microbiota of cultured european sea bass (dicentrarchus labrax) fed two functional diets applying pyrosequencing of pcr-amplified 16s rrna gene. quality-filtered reads were assigned to ... | 2014 | 24730648 |
| farming-up coastal fish assemblages through a massive aquaculture escape event. | we investigated the changes on the mean trophic level of fish assemblages across different spatiotemporal scales, before and after a massive escape event occurred off la palma (canary islands), which resulted in the release of 1.5 million fish (mostly dicentrarchus labrax) into the wild. the presence of escaped fish altered significantly the mean trophic level of fish assemblages in shallow coastal waters. this alteration was exacerbated by the massive escape. a nearby marine protected area buff ... | 2014 | 24725509 |
| exogenous expression of marine lectins dlfbl and sprbl induces cancer cell apoptosis possibly through prmt5-e2f-1 pathway. | lectins are widely existed in marine bioresources, and some purified marine lectins were found toxic to cancer cells. in this report, genes encoding dicentrarchus labrax fucose-binding lectin (dlfbl) and strongylocentrotus purpuratus rhamnose-binding lectin (sprbl) were inserted into an adenovirus vector to form ad.flag-dlfbl and ad.flag-sprbl, which elicited significant in vitro suppressive effect on a variety of cancer cells. anti-apoptosis factors bcl-2 and xiap were determined to be downregu ... | 2014 | 24675921 |
| erod activity and antioxidant defenses of sea bass (dicentrarchus labrax) after an in vivo chronic hydrocarbon pollution followed by a post-exposure period. | chronic concentrations of polycyclic aromatic hydrocarbons (pahs) have been commonly detected in international estuaries ecosystems. reliable indicators still need to be found in order to properly assess the impact of pahs in fish. after an in vivo chronic exposure to hydrocarbons, the enzymatic activity of 7-ethoxyresorufin o-deethylase (erod) and the antioxidant defense system were assessed in sea bass, dicentrarchus labrax. a total of 45 fish were exposed to the water-soluble fraction of arab ... | 2014 | 24659404 |
| blood chemistry profile as indicator of nutritional status in european seabass (dicentrarchus labrax). | this study was carried out to establish biochemical parameters with potential diagnostic value to assess the nutritional status of healthy seabass. for that purpose, triplicate groups of seabass juveniles were submitted to different feeding protocols: fed for 14 days; fed for 7 days followed by 7 days of fasting or fasted for 14 days. at the end of the trial, body, liver and viscera were randomly sampled for proximate composition analysis. blood was also collected and the following plasma parame ... | 2014 | 24647468 |
| characterization, tissue distribution and regulation by fasting of the agouti family of peptides in the sea bass (dicentrarchus labrax). | the melanocortin system is one of the most complex hormonal systems in vertebrates. atypically, the signaling of melanocortin receptors is regulated by the binding of endogenous antagonists, named agouti-signaling protein (asip) and agouti-related protein (agrp). teleost specific genome duplication (tsgd) rendered new gene copies in teleost fish and up to four different genes of the agouti family of peptides have been characterized. in this paper, molecular cloning was used to characterize mrna ... | 2014 | 24561275 |
| iron bioavailability in larvae yellow snapper (lutjanus argentiventris): cloning and expression analysis of ferritin-h. | ferritin is a major intracellular iron storage protein in higher vertebrates and plays an important role in iron metabolism. in this study, ferritin h subunit was cloned from the larvae of yellow snapper, lutjanus argentiventris, by rapid amplification of cdna ends (race) following in silico transcriptome analysis. the full-length cdnas of the lafeh was 1231 bp in length encoding 177 amino acids with a predicted molecular mass (mw) about 20.82 kda and theoretical isoelectric point (pi) of 5.79. ... | 2014 | 24561126 |
| in vitro effect of cadmium and copper on separated blood leukocytes of dicentrarchus labrax. | the immunotoxic effects of heavy metals on blood leukocytes of sea bass (dicentrarchus labrax) were examined. the cells, separated by a discontinuous percoll-gradients, were exposed in vitro to various sublethal concentrations of cadmium and copper (10(-7)m, 10(-5)m, and 10(-3)m) and their immunotoxic effect was then evaluated by measuring neutral red uptake, mtt assay, dna fragmentation and hsp70 gene expression. first of all, we demonstrated that the cells treated in vitro could incorporate cd ... | 2014 | 24530726 |
| acute toxicity of a commercial glyphosate formulation on european sea bass juveniles (dicentrarchus labrax l.): gene expressions of heme oxygenase-1 (ho-1), acetylcholinesterase (ache) and aromatases (cyp19a and cyp19b). | acute toxicity of roundup, a commercial glyphosate--based herbicide, was evaluated in a teleost marine fish, the european sea bass, after 96 h of exposure. the lc50 96-h value of roundup was 529 mg/l. juveniles (dicentrarchus labrax l.) were exposed to a sublethal concentration (35% of the lc50, i.e. 193 mg/l) of roundup for 96-h. the study of heme oxygenase-1 (ho-1) gene expression was performed in four tissues (liver, gills, brain and gonads) and highlighted the disruption of antioxidant defen ... | 2013 | 24461331 |
| effect of a glyphosate-based herbicide on gene expressions of the cytokines interleukin-1β and interleukin-10 and of heme oxygenase-1 in european sea bass, dicentrarchus labrax l. | glyphosate-based herbicides are the most frequently used herbicides in the world. we evaluated the effect of roundup 360 sl on the expression of interleukin-1β (il-1β), interleukin-10 (il-10) and heme-oxygenase-1 (ho-1) in the gills, intestines and spleen of young european sea bass (dicentrachus labrax l.), aged 8 mo. a group of fish was exposed to 647 mg/l of roundup for 96 h. this treatment did not alter gene expression levels of il-1β and il-10 cytokine in the intestines, but significantly lo ... | 2014 | 24408037 |
| protocol for quantitative shape analysis of deformities in early larval european seabass dicentrarchus labrax. | this study established an optimized protocol for quantifying the shape variation of newly hatched larvae of european seabass dicentrarchus labrax, focusing on the effect of fixatives and mounting on body shape from hatching until 14 days post hatching, while also minimizing the error introduced by handling. this assessment was performed based on both biometric and geometric shape data, with the latter relying on outline based elliptic fourier analysis. the fixation and mounting effect on the tot ... | 2014 | 24383805 |
| comparative study of pineal clock gene and aanat2 expression in relation to melatonin synthesis in atlantic salmon (salmo salar) and european seabass (dicentrarchus labrax). | the photoreceptive teleost pineal is considered to be essential to the generation, synchronisation and maintenance of biological rhythms, primarily via melatonin release. the role of internal (circadian clock) and external (light) signals controlling melatonin production in the fish pineal differs between species, yet the reasons underpinning this remain largely unknown. whilst in salmonids, pineal melatonin is apparently regulated directly by light, in all other studied teleosts, rhythmic melat ... | 2014 | 24361868 |
| diel changes in plasma cortisol and effects of size and stress duration on the cortisol response in european sea bass (dicentrarchus labrax). | european sea bass (dicentrarchus labrax), one of the most economically important fish in mediterranean mariculture, shows high basal cortisol concentrations compared with other teleosts. the present study aims (a) to identify cortisol diel variation in fish held under a 12l:12d cycle and minimum handling stress, and (b) to establish the effect of fish size and stressor duration on the cortisol response. the results indicate high intrapopulation variability in plasma cortisol and a significant di ... | 2014 | 24343759 |
| the first demonstration of cyp1a and the abc protein(s) gene expression and activity in european seabass (dicentrarchus labrax) primary hepatocytes. | primary hepatocytes are a model for studying various effects of different xenobiotics, including detoxification strategies. in this study we have isolated and cultured european seabass (dicentrarchus labrax) primary hepatocytes and assessed gene transcription and activity of cyp1a (phase i of cellular detoxification) and abcc1 and abcc2 (phase iii) transport proteins after exposure to benzo(a)pyrene (bap). a dose dependent increase in abcc2 and cyp1a mrna transcripts was observed in seabass prim ... | 2014 | 24342362 |
| fast growers sprint slower: effects of food deprivation and re-feeding on sprint swimming performance in individual juvenile european sea bass. | while many ectothermic species can withstand prolonged fasting without mortality, food deprivation may have sublethal effects of ecological importance, including reductions in locomotor ability. little is known about how such changes in performance in individual animals are related to either mass loss during food deprivation or growth rate during re-feeding. this study followed changes in the maximum sprint swimming performance of individual european sea bass, dicentrarchus labrax, throughout 45 ... | 2014 | 24265431 |
| administration of follicle-stimulating hormone in vivo triggers testicular recrudescence of juvenile european sea bass (dicentrarchus labrax). | follicle-stimulating hormone (fsh) is thought to act early in the process of spermatogenesis; however, its action in fish has not yet been clearly established. in the present work, we analyzed the effects of recombinant fsh in sea bass (dicentrarchus labrax) spermatogenesis according to two different approaches: direct injection of recombinant single-chain fsh hormone (scfsh) and injection of scfsh coding sequence. both approaches were efficient in increasing plasma fsh at 7 and 15 days, respect ... | 2014 | 24258209 |
| characterization of the environmental quality of sediments from two estuarine systems based on different in-vitro bioassays. | this study characterizes the environmental quality of sediments from the arade and guadiana estuaries using different in-vitro bioassays: a) fish hepatoma cell line (plhc-1) to determine cytotoxicity and presence of cyp1a and oxidative stress inducing agents; b) gonad subcellular fractions from sea bass (dicentrarchus labrax) to detect compounds that are likely to act as endocrine disrupters by interfering with the synthesis of androgens (cyp17, cyp11β) and estrogens (cyp19). approximately 60% o ... | 2014 | 24140013 |
| structural elucidation of olive pomace fed sea bass (dicentrarchus labrax) polar lipids with cardioprotective activities. | the purpose of this study was to structurally characterise the polar lipids of sea bass (dicentrarchus labrax), fed with an experimental diet containing olive pomace (op), that exhibit cardioprotective activities. op has been added to conventional fish oil (fo) feed at 4% and this was the op diet, having been supplemented as finishing diet to fish. sea bass was aquacultured using either fo or op diet. at the end of the dietary experiment, lipids in both samples of fish muscle were quantified and ... | 2014 | 24128590 |
| ultrasound measurement of reproductive organs in juvenile european sea bass dicentrarchus labrax. | in this study, ultrasonographic examination was performed thrice, 15 days apart, on juvenile european sea bass dicentrarchus labrax, from 330 to 360 days of age, to assess the size and the morphology of male and female. results have proved this method as a suitable and non-invasive procedure to assess sexual differentiation. | 2013 | 24117993 |
| proteomic profiling of sea bass muscle by two-dimensional gel electrophoresis and tandem mass spectrometry. | in this study, the proteome profile of european sea bass (dicentrarchus labrax) muscle was analyzed using two-dimensional electrophoresis (2-de) and tandem mass spectrometry with the aim of providing a more detailed characterization of its specific protein expression profile. a highly populated and well-resolved 2-de map of the sea bass muscle tissue was generated, and the corresponding protein identity was provided for a total of 49 abundant protein spots. upon ingenuity pathway analysis, the p ... | 2014 | 24057758 |
| foxl2 and foxl3 are two ancient paralogs that remain fully functional in teleosts. | foxl2 is a forkhead transcription factor involved in mammalian development and regulation of reproduction. two foxl2 paralogs, foxl2a and foxl2b, have been described in various teleost species and were considered as fish-specific duplicates. here, we report the isolation and characterization of foxl2a (foxl2) and foxl2b (foxl3) in european sea bass (dicentrarchus labrax), together with the identification of these two genes in non-teleost genomes. phylogenetic and synteny analyses indicate that t ... | 2013 | 24045113 |
| relationships among traits of aerobic and anaerobic swimming performance in individual european sea bass dicentrarchus labrax. | teleost fishes exhibit wide and temporally stable inter-individual variation in a suite of aerobic and anaerobic locomotor traits. one mechanism that could allow such variation to persist within populations is the presence of tradeoffs between aerobic and anaerobic performance, such that individuals with a high capacity for one type of performance have a reduced capacity for the other. we investigated this possibility in european seabass dicentrarchuslabrax, each measured for a battery of indica ... | 2013 | 24019879 |
| ketoconazole modulates the infectivity of ichthyophonus sp. (mesomycetozoa) in vivo in experimentally injected european sea bass. | in vitro studies have confirmed the inhibitory effect of the azol-derivative ketoconazole (kz) on the growth of ichthyophonus, an important pathogen causing epizootics in wild and cultured fish. we evaluated the effect of kz in vivo in european sea bass dicentrarchus labrax experimentally infected with the same ichthyophonus isolate. liposomes were used to vehiculate different doses of kz to increase the effect on ichthyophonus and lower the toxicity of the drug, and kz toxicity was assessed in ... | 2013 | 23999706 |
| mercury (hg) and methyl mercury (mehg) concentrations in fish from the coastal lagoon of orbetello, central italy. | total mercury (hg tot) and methyl mercury (mehg) were quantified in several specimens of dicentrarchus labrax and sparus aurata from the east basin of the orbetello lagoon, central italy. the size of each specimen was recorded to estimate body burdens (bbs); =hg tot and mehg were measured in fillets of both species. hg tot and mehg in s. aurata ranged between 0.355-1.58 and 0.341-1.53 μg/g wet weight (ww), respectively; in d. labrax, their ranges were 0.284-2.54 and 0.214-2.35 μg/g ww. approxima ... | 2013 | 23998853 |
| behavioural responses of sardines sardina pilchardus to simulated purse-seine capture and slipping. | the behavioural effects of confinement of sardine sardina pilchardus in a purse seine were evaluated through three laboratory experiments simulating the final stages of purse seining; the process of slipping (deliberately allowing fishes to escape) and subsequent exposure to potential predators. effects of holding time (the time s. pilchardus were held or entangled in the simulation apparatus) and s. pilchardus density were investigated. experiment 1 compared the effect of a mild fishing stresso ... | 2013 | 23991869 |
| dax1 regulatory networks unveil conserved and potentially new functions. | dax1 is an orphan nuclear receptor with actions in mammalian sex determination, regulation of steroidogenesis, embryonic development and neural differentiation. conserved patterns of dax1 gene expression from mammals to fish have been taken to suggest conserved function. in the present study, the european sea bass, dicentrarchus labrax, dax1 promoter was isolated and its conserved features compared to other fish and mammalian dax1 promoters in order to derive common regulators and functional gen ... | 2013 | 23954228 |
| biological responses of juvenile european sea bass (dicentrarchus labrax) exposed to contaminated sediments. | multiple anthropogenic activities present along coastal environments may affect the health status of aquatic ecosystems. in this study, specimens of european sea bass (dicentrarchus labrax) were exposed for 30 days to highly contaminated sediment collected from the industrial area between augusta and priolo (syracuse, italy), defined as the most mercury polluted site in the mediterranean. the aim was to evaluate the responses of juvenile d. labrax to highly contaminated sediments, particularly e ... | 2013 | 23953925 |
| effect of dietary herbal supplements on some physiological conditions of sea bass dicentrarchus labrax. | this study was conducted in order to investigate the effects of dietary thyme thymus vulgaris, rosemary rosmarinus officinalis,and fenugreek trigonella foenum graecum as feed additives on total liver fat levels and biometric indices of sea bass dicentrarchus labrax. four isonitrogenous (48% crude protein) and isocaloric(21 kj/g) diets were formulated to contain 0% (control), or 1% of thyme, rosemary, or fenugreek. in a 45-d feeding trial, 12 fiberglass tanks (140 l) were each stocked with 17 fis ... | 2013 | 23914399 |
| two thioredoxin-superfamily members from sea bass (dicentrarchus labrax, l.): characterization of pdi (pdia1) and erp57 (pdia3). | pdi (pdia1) and erp57 (pdia3), members of the pdi family and of the thioredoxin (trx) superfamily, are multifunctional proteins with wide physiological roles and have been implicated in several pathologies. importantly, they are both involved in the mhc class i antigen presentation pathway. this paper reports the isolation and characterization of full cdna and genomic clones from sea bass (dicentrarchus labrax, l.) pdi (dila-pdi) and erp57 (dila-erp57). the genes are ~12.4 and ~7.1 kb long, orig ... | 2013 | 23880452 |
| effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in european seabass (dicentrarchus labrax l.). | sources of blood glucose in european seabass (initial weight 218.0±43.0g; mean±s.d., n=18) were quantified by supplementing seawater with deuterated water (5%-(2)h2o) for 72h and analyzing blood glucose (2)h-enrichments by (2)h nmr. three different nutritional states were studied: continuously fed, 21-day of fast and 21-day fast followed by 3days of refeeding. plasma glucose levels (mm) were 10.7±6.3 (fed), 4.8±1.2 (fasted), and 9.3±1.4 (refed) (means±s.d., n=6), showing poor glycemic control. f ... | 2013 | 23871878 |
| rapd-pcr analysis for molecular characterization and genotoxic studies of a new marine fish cell line derived from dicentrarchus labrax. | continuous cell lines could provide an important tool for studying epidemiology, toxicology, cellular physiology and the host-pathogen interactions. random amplified polymorphic deoxyribonucleic acid analysis by pcr (rapd-pcr) was used for the molecular characterization of dicentrarchus labrax embryonic cells (dlec) as a possible tool to detect dna alterations in environmental genotoxic studies. we studied the dna pattern of the dlec fish cell line, a fibroblast-like cell line derived from europ ... | 2014 | 23839298 |
| disposition of [u-2h7]glucose into hepatic glycogen in rat and in seabass. | the stimulation of hepatic glycogenesis is a ubiquitous response to a glucose challenge and quantifying its contribution to glucose uptake informs its role in restoring euglycemia. glycogenesis can be quantified with labeled water provided that exchange of glucose-6-phosphate hydrogen 2 (g6p-h2) and body water via glucose-6-phosphate isomerase, and exchange of positions 4, 5 and 6 hydrogens (g6p-h456) via transaldolase, are known. these exchanges were quantified in 24-h fasted rats (rattus norve ... | 2013 | 23838145 |
| first insight into exploration and cognition in wild caught and domesticated sea bass (dicentrarchus labrax) in a maze. | european sea bass aquaculture is so recent that very little is known on the effects of the early steps of its domestication. behavioural parameters are sensitive indicators of the domestication process since they are generally impacted as soon as the first generation. the present work compared wild-caught and domesticated sea bass juvenile swimming activity, exploration and ability to learn to discriminate between two 2-d objects associated to a simple spatial task that enabled the tested indivi ... | 2013 | 23805190 |
| influence of temperature in thermal and oxidative stress responses in estuarine fish. | the influence of increasing temperatures in thermal and oxidative stress responses were studied in the muscle of several estuarine fish species (diplodus vulgaris, diplodus sargus, dicentrarchus labrax, gobius niger and liza ramada). selected fish were collected in july at the tagus estuary (24±0.9°c; salinity of 30±4‰; ph=8). fish were subjected to a temperature increase of 1°c.h(-1) until they reached their critical thermal maximum (ctmax), starting at 24°c (control temperature). muscle sample ... | 2013 | 23774589 |
| enteroendocrine profile of α-transducin immunoreactive cells in the gastrointestinal tract of the european sea bass (dicentrarchus labrax). | in vertebrates, chemosensitivity of nutrients occurs through the activation of taste receptors coupled with g-protein subunits, including α-transducin (g(αtran)) and α-gustducin (g(αgust)). this study was aimed at characterising the cells expressing g(αtran) immunoreactivity throughout the mucosa of the sea bass gastrointestinal tract. g(αtran) immunoreactive cells were mainly found in the stomach, and a lower number of immunopositive cells were detected in the intestine. some g(αtran) immunorea ... | 2013 | 23748963 |
| effects of dopaminergic system activation on feeding behavior and growth performance of the sea bass (dicentrarchus labrax): a self-feeding approach. | dopamine is synthesized from l-dopa and subsequently processed into norepinephrine and epinephrine. any excess neurotransmitter can be taken up again by the neurons to be broken down enzymatically into dopac. the effect of dopamine on mammalian food intake is controversial. mice unable to synthesize central dopamine die of starvation. however, studies have also shown that central injection of dopamine inhibits food intake. the effect of dopaminergic system in the fish feeding behavior has been s ... | 2013 | 23747830 |
| effect of seasonal changes on the gelling properties of farmed sea bass (dicentrarchus labrax). | the effect of seasonal changes (summer versus winter) upon the quality of heat-induced gel products from farmed sea bass (dicentrarchus labrax) was studied. moreover, the effect of microbial transglutaminase addition (0.5%, w/w) on the quality was assessed. fat content of gel products attained from sea bass grown during summer was higher (12.0% versus 7.6%). textural properties of the gels from the summer fish were higher. protein of gels from winter fish was less soluble in sds + dtt (sodium do ... | 2014 | 23733821 |
| persistent organic pollutants in sea bass (dicentrarchus labrax l.) in two fish farms in the mediterranean sea. | we analyzed polychlorobiphenyls (pcbs), perfluorooctanesulfonate (pfos) and perfluorooctanoate (pfoa) in the edible part of farmed sea bass reared in two fish farms in liguria (northern italy). the aim was to determine the contamination levels and human exposure to these chemicals through fish consumption. concentrations of "dioxin-like" pcbs (dl-pcbs) ranged from 0.033 to 0.759pg σteq-pcbg(-1) whole weight (w.w.) in fish farm 1 and from 0.032 to 1.60pg σteq-pcbg(-1) w.w. in fish farm 2, and the ... | 2013 | 23726013 |
| the responses of the hepatosomatic index (hsi), 7-ethoxyresorufin-o-deethylase (erod) activity and glutathione-s-transferase (gst) activity in sea bass (dicentrarchus labrax, linnaeus 1758) caged at a polluted site: implications for their use in environmental risk assessment. | the present study investigates the response of three hepatic biomarkers in adult sea bass (dicentrarchus labrax, linnaeus 1758) caged at a wastewater outlet of an oil refinery with fish caged at a pristine site used as controls. the biomarkers that were investigated were the hepatosomatic index (hsi), 7-ethoxyresorufin-o-deethylase (erod) activity and glutathione-s-transferase (gst) activity. in addition, we have measured the levels of polycyclic aromatic hydrocarbons (pahs) and selected heavy m ... | 2013 | 23644668 |
| 17β-estradiol induces changes in cytokine levels in head kidney and blood of juvenile sea bass (dicentrarchus labrax, l., 1758). | the cytokine network is involved in the immune system communication. as estrogens influence the cytokine expression in mammals, this study investigated the impact of exogenous estrogenic pollutants on selected cytokines in dicentrarchus labrax. the gene expression of interleukin 6, tumour necrosis factor α, transforming growth factor β1 and interleukin 1β was assessed and accomplished with protein measurements in the blood for the last two. impacts through 17β-estradiol mainly occurred at the be ... | 2013 | 23602341 |
| presence of contracaecum spp. in teleosts cultured and fished in sardinia. | this study reports the results of the finding of contracaecum spp. during a survey on endoparasites isolated from cultured and wild fish and also from some cephalopods caught in sardinian waters. contracaecum spp. is a nematode belonging to the anisakidae, and is reported to cause zoonosis in humans. nematodes were detected after visual inspection and enzymatic digestion and then identified by morphologic observation, which was confirmed by pcr. the results show that contracaecum spp. were found ... | 2013 | 23597773 |
| expression of kisspeptins and their receptors, gnrh-1/gnrhr-ii-1a and gonadotropin genes in the brain of adult male and female european sea bass during different gonadal stages. | kisspeptins play a critical role in the control of hypothalamic-gonadotropic function and puberty onset in mammals. studies in fish have all supported the hypothesis that they might play similar roles in the reproduction of this animal group, however, their physiological relevance in the occurrence of key reproductive events still remains to be determined. this study examines the relative mrna expression profiles of the duplicate kisspeptin system (kiss1, kiss2, gpr54-1b, and gpr54-2b) in the hy ... | 2013 | 23583767 |
| effect of two sulfur-containing amino acids, taurine and hypotaurine in european sea bass (dicentrarchus labrax) sperm cryopreservation. | in the present work, taurine and hypotaurine were evaluated as potential additives to improve european sea bass (dicentrarchus labrax) sperm quality after cryopreservation. for cryopreservation, three different extenders were used: control extender (nam), supplemented with 1mm taurine or supplemented with 1mm hypotaurine, all of them containing 10% me₂so as cryoprotectant. to evaluate sperm quality of fresh and thawed sperm, motility (casa: computer assisted sperm analysis), viability (sybr gree ... | 2013 | 23583301 |
| melatonin inhibits gnrh-1, gnrh-3 and gnrh receptor expression in the brain of the european sea bass, dicentrarchus labrax. | several evidences supported the existence of melatonin effects on reproductive system in fish. in order to investigate whether melatonin is involved in the modulation of gnrh systems in the european sea bass, we have injected melatonin (0.5 µg/g body mass) in male specimens. the brain mrna transcript levels of the three gnrh forms and the five gnrh receptors present in this species were determined by real time quantitative pcr. our findings revealed day-night variations in the brain expression o ... | 2013 | 23567273 |
| involvement of dietary salt in shaping bacterial communities in european sea bass (dicentrarchus labrax). | bacteria associated with the digestive tract of multicellular organisms have been shown to play a major role in their hosts' functioning. in fish, it has been proposed that food fermentation occurs inside the pyloric ceca, pouch like organs found in their digestive tract. however, this notion remains controversial. furthermore, changes in pyloric cecal bacterial populations under different diets have yet to be demonstrated in fish. in this study, we explore the changes occurring in the bacterial ... | 2013 | 23558231 |
| two mx genes identified in european sea bass (dicentrarchus labrax) respond differently to vnnv infection. | mx proteins are key components of the antiviral state triggered by interferon type i in response to viral infections. in this study, two different mx genes have been identified in european sea bass (dicentrarchus labrax), and their sequences were cloned and characterized. mxa cdna consists of 1881 bp coding for a putative 626 aminoacids protein, while mxb cdna has 1920 bp and results in a protein with 639 residues. their corresponding genomic sequences contain 3538 bp and 5326 bp, respectively, ... | 2013 | 23548865 |
| enhanced intestinal epithelial barrier health status on european sea bass (dicentrarchus labrax) fed mannan oligosaccharides. | the study assesses the effects of dietary mannan oligosaccharides (mos) in european sea bass (dicentrarchus labrax) posterior intestinal lipid class composition and its possible relation to the potential prostaglandins production and gut associated lymphoid tissue (galt) stimulation. fish were fed 4 g kg(-1) mos (bio-mos(®) aquagrade, alltech, inc., usa) for eight weeks. fish fed mos presented higher (p ≤ 0.05) weight gain, total length, and specific and relative growth rates than fish fed the c ... | 2013 | 23528875 |
| molecular cloning and characterization of sea bass (dicentrarchus labrax, l.) calreticulin. | mammalian calreticulin (crt) is a key molecular chaperone and regulator of ca(2+) homeostasis in endoplasmic reticulum (er), also being implicated in a variety of physiological/pathological processes outside the er. importantly, it is involved in assembly of mhc class i molecules. in this work, sea bass (dicentrarchus labrax) crt (dila-crt) gene and cdna have been isolated and characterized. the mature protein retains two conserved motifs, three structural/functional domains (n, p and c), three ... | 2013 | 23523749 |
| regulation of natural killer enhancing factor (nkef) genes in teleost fish, gilthead seabream and european sea bass. | peroxiredoxins (prx) are a family of antioxidant proteins also involved in inflammation and innate immunity. prx1 and prx2 are also known as natural killer enhancing factor (nkef)-a and nkef-b, respectively, by their ability to prime the mammalian nk-cells activity. in teleost fish, nkef genes have been isolated but their regulation has been scarcely evaluated. we have identified orthologues of the nkef-a and nkef-b genes in the teleost european sea bass (dicentrarchus labrax) which showed const ... | 2013 | 23511025 |
| starvation and re-feeding affect hsp expression, mapk activation and antioxidant enzymes activity of european sea bass (dicentrarchus labrax). | in the context of food deprivation in fish (wild and farmed), understanding of cellular responses is necessary in order to develop strategies to minimize stress caused by starvation in the aquaculture section. the present study evaluates the effects of long term starvation (1f-3s: one-month feeding-three-month starvation) and starvation/re-feeding (2s-2f: two-month starvation-two-month re-feeding) compared to the control group (4f-0s: four-month feeding-zero month starvation) on cellular stress ... | 2013 | 23462223 |
| changes of enzymes activity and protein profiles caused by high-pressure processing in sea bass (dicentrarchus labrax) fillets. | high-pressure processing (hpp) is a technology of growing interest for food preservation, due to its ability to control the activity of degradative enzymes. the effect of three variables (pressure levels of 100, 250, and 400 mpa; pressure holding times of 0, 5, 15, and 30 min; and pressurization rates of 8 and 14 mpa/s) on the activity of the enzymes acid phosphatase, cathepsins (b and d), lipase, and calpains was studied using sea bass fillets as a case study model. additionally, the effect of ... | 2013 | 23425724 |
| effects of oil exposure and dispersant use upon environmental adaptation performance and fitness in the european sea bass, dicentrarchus labrax. | the worldwide increasing recourse to chemical dispersants to deal with oil spills in marine coastal ecosystems is a controversial issue. yet, there exists no adequate methodology that can provide reliable predictions of how oil and dispersant-treated oil can affect relevant organism or population-level performance. the primary objective of the present study was to examine and compare the effects of exposure to untreated oil (weathered arabian light crude oil), chemically dispersed oil (finasol, ... | 2013 | 23411352 |
| influence of pre-slaughtering feed restriction on muscle characteristics of farmed sea bass (dicentrarchus labrax l.) during cold storage. | this paper deals with the consequences of dietary restriction or complete starvation before slaughtering on the biochemical and textural characteristics of sea bass muscle. | 2013 | 23400825 |
| viral encephalopathy and retinopathy in groupers (epinephelus spp.) in southern italy: a threat for wild endangered species? | betanodaviruses are the causative agents of viral encephalopathy and retinopathy (ver). to date, more than 50 species have proved to be susceptible and among them, those found in genus epinephelus are highly represented. clinical disease outbreaks are generally characterized by typical nervous signs and significant mortalities mainly associated with aquaculture activities, although some concerns for the impact of this infection in wild fish have been raised. in this study, the authors present th ... | 2013 | 23351980 |
| gene flow at major transitional areas in sea bass (dicentrarchus labrax) and the possible emergence of a hybrid swarm. | the population genetic structure of sea bass (dicentrarchus labrax) along a transect from the atlantic ocean (ao) to the eastern mediterranean (em) sea differs from that of most other marine taxa in this area. three populations (ao, western mediterranean [wm], em) are recognized today, which were originally two allopatric populations. how two ancestral genetic units have evolved into three distinct units has not been addressed yet. therefore, to investigate mechanisms that lead to the emergence ... | 2012 | 23301173 |
| the clc-3 chloride channel and osmoregulation in the european sea bass, dicentrarchus labrax. | dicentrarchus labrax migrates between sea (sw), brackish and fresh water (fw) where chloride concentrations and requirements for chloride handling change: in fw, fish absorb chloride and restrict renal losses; in sw, they excrete chloride. in this study, the expression and localization of clc-3 and na(+)/k(+)-atpase (nka) were studied in fish adapted to sw, or exposed to fw from 10 min to 30 days. in gills, nka-α1 subunit expression transiently increased from 10 min and reached a stabilized inte ... | 2013 | 23292336 |
| metabolism of the polycyclic musk galaxolide and its interference with endogenous and xenobiotic metabolizing enzymes in the european sea bass (dicentrarchus labrax). | this study investigates the metabolism and mode of action of galaxolide (hhcb) in the european sea bass -dicentrarchus labrax- following a single intraperitoneal injection of 50 mg hhcb/kg body weight. in addition, a group of fish was injected with 50 mg/kg of ketoconazole (kcz), a fungicide that is known to interfere with different cyp isoenzymes. hhcb was actively metabolised by sea bass and acted as a weak inhibitor of the synthesis of oxyandrogens in gonads of male fish. both, hhcb and a hyd ... | 2013 | 23274450 |
| multiple vitellogenin yolk precursors in european sea bass (dicentrarchus labrax). | | 2013 | 25141754 |
| microparticles delivery system in gnotobiotic european sea bass larvae (dicentrarchus labrax, l. 1758). | | 2013 | 25141751 |
| cholecystokinin and tryptic enzyme activity in sea bass (dicentrarchus labrax) larvae: a regulatory loop and the impact of feeding regimes. | | 2013 | 25141739 |
| use of phaeobacter sp. probiotic bacteria for the rearing of sea bass larvae (dicentrarchus labrax). | | 2013 | 25141684 |
| correlation of saddleback syndrome with deformities of the pelvic fins and lateral line in european sea bass, dicentrarchus labrax. | | 2013 | 25141647 |
| oxidative stress in sea bass dicentrarchus labrax larvae: interaction of high dietary dha contents and several antioxidant nutrients. | | 2013 | 25141617 |
| consumer preferences regarding the introduction of new organic products. the case of the mediterranean sea bass (dicentrarchus labrax) in italy. | the introduction of new products on the market poses several challenges; in particular, whether the characteristics of the proposed product will be judged positively by potential consumers. this paper analyses the preferences of consumers regarding the introduction on the italian market of a new product: organic mediterranean sea bass. the aim of this study is to assess the importance given by consumers to four main characteristics of sea bass (country of origin, size, production method - organi ... | 2013 | 23268110 |
| sarcoplasmic reticulum: a key factor in cardiac contractility of sea bass dicentrarchus labrax and common sole solea solea during thermal acclimations. | this study investigated the effects of acclimation temperature upon (i) contractility of ventricular strips (ii) calcium movements in ventricular cardiomyocytes during excitation-contraction coupling (ecc), and (iii) the role of the sarcoplasmic reticulum (sr) in myocardial responses, in two marine teleosts, the sea bass (dicentrarchus labrax) and the common sole (solea solea). because of the different sensitivities of their metabolism to temperature variation, both species were exposed to diffe ... | 2013 | 23263664 |
| luteinizing hormone plasmid therapy results in long-lasting high circulating lh and increased sperm production in european sea bass (dicentrarchus labrax). | the present work aimed at evaluating the potential of intramuscular injection of a hormone-coding gene as an approach for gene therapy in fish. a plasmid containing luteinizing hormone (lh) in a single-chain (sc) form, pcmv-sclh, was chosen as the coding gene, and sea bass was chosen as the target species. in vivo injection of pcmv-sclh in muscle of juvenile sea bass rendered plasma lh levels higher than 50 ng/ml in 40% of the injected fish, while these lh levels were only detected in 4% of cont ... | 2013 | 23255338 |
| fatty acid metabolism in european sea bass (dicentrarchus labrax): effects of n-6 pufa and mufa in fish oil replaced diets. | monounsaturated fatty acids (mufa)-rich and n-6 polyunsaturated fatty acid (n-6 pufa)-rich vegetable oils are increasingly used as fish oil replacers for aquafeed formulation. the present study investigated the fatty acid metabolism in juvenile european sea bass (dicentrarchus labrax, 38.4 g) fed diets containing fish oil (fo, as the control treatment) or two different vegetable oils (the mufa-rich canola/rapeseed oil, co; and the n-6 pufa-rich cottonseed oil, cso) tested individually or as a 50 ... | 2013 | 23212581 |
| physiological pathways involved in nutritional muscle dystrophy and healing in european sea bass (dicentrarchus labrax) larvae. | the potential muscle regeneration after nutritional dystrophy caused by high dietary dha contents in fish and the physiological pathways involved are still unknown. to better understand this process, an experiment was conducted for 3 weeks in 14 day-old european sea bass larvae using different dha ratios (1 or 5%). after this period, part of the larvae fed 5% dha diet was switched to 1% dha diet ("wash-out") for another 2 weeks. larvae fed 5% dha diet showed altered oxidative status as indicated ... | 2013 | 23202658 |