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eliminating parvovirus b19 from blood products. 19947907759
subclinical transplacental parvovirus b19 infection: an increased fetal risk? 19947909117
parvovirus b19 and blood products. 19947909118
parvovirus b19 and blood products. 19947909119
chronic parvovirus b19 infection and systemic necrotising vasculitis: opportunistic infection or aetiological agent?we describe three patients who had infection with human parvovirus b19 in association with new-onset systemic necrotising vasculitis syndromes, two with features of polyarteritis nodosa and one with features of wegener's granulomatosis. chronic b19 infection, lasting 5 months to more than 3 years, was shown by enzyme immunoassay for igg and igm antibodies to b19 and polymerase chain reaction for b19 dna in serum and tissue samples. the patients had atypical serological responses to the b19 infec ...19947910276
active or recent parvovirus b19 infection in children with kawasaki disease.because parvovirus b19 occasionally causes some of the features typical of kawasaki disease, we investigated b19 involvement in 15 children with kawasaki disease. active or recent b19 infection, as shown by b19-dnaaemia, positive b19-specific igm antibodies, or both, was diagnosed in 10 patients (67%). a high frequency of all major criteria for diagnosis of kawasaki disease (60%), anaemia (60%), coronary aneurysms (30%), and arthropathy (30%) was found in children with b19-associated kawasaki di ...19947910278
human parvovirus b19 after concentrates for haemophilia. 19947911884
human parvovirus b19 infection in kawasaki disease. 19947912322
human parvovirus b19 infection in kawasaki disease. 19947912323
polyarteritis nodosa and parvovirus b19. 19947913176
transient disturbance of consciousness and hepatic dysfunction associated with human parvovirus b19 infection. 19947914998
dual infection with human herpesvirus type 6 and parvovirus b19 in a renal transplant recipient.a 7-year-old boy developed intermittent fever and rashes 2 weeks after anti-lymphocyte globulin therapy for a transplant rejection episode. dual infection with parvovirus b19 and human herpesvirus type 6 was suggested by serological tests. neither virus has been reported previously in a symptomatic paediatric renal transplant recipient.19947917866
parvovirus b19 outbreak on an adult ward.in november and december 1992, an outbreak of parvovirus b19 infection occurred among patients and staff on an adult mixed surgical ward at a large hospital in london. three patients and 15 staff members were serologically confirmed as acute cases. the attack rate among susceptible members of staff was 47%. in those infected, arthralgia (80%) and rash (67%) were the most common symptoms. of six susceptible in-patients on the ward, three became infected. one of the in-patients who had carcinoma o ...19947925671
cytopenia caused by parvovirus in an adult all patient.a young woman in maintenance therapy for acute lymphoblastic leukemia in second complete remission developed fever and a skin rash associated with severe anemia, neutropenia and erythroblastopenia. a complete recovery was obtained in 4 weeks' time after red cell transfusion, i.v. immunoglobulin and withdrawal of the maintenance chemotherapy. parvovirus b19 infection was demonstrated by detection of b19 dna in the patient's serum using a dot-blot hybridization assay and a nested polymerase chain ...19947926975
use of polymerase chain reaction for detection of human parvovirus b19. 19947930708
[five family members infection likely due to human parvovirus b19 (erythema infectiosum)].we encountered five members of the same family infected with parvovirus b19. the latency period in these patients was 17 to 20 days, which matched that observed by anderson et al. after inoculating humans with the virus. the latency period in 24 cases of familial infection observed in 17 families at the same time showed peaks at both 10 days and 20 days. thus, it is still necessary to determine the actual latent period of erythema infectiosum infection.19947930793
chronic modulation of the autoimmune response following parvovirus b19 infection.we describe 2 patients with prolonged autoimmune alterations following parvovirus b19 infection. b19 induced aplastic crises were the revealing manifestations of asymptomatic hemolytic conditions in the 2 patients: a coombs' positive hemolytic anemia induced by d-penicillamine in the first and congenital spherocytosis in the second. both patients had transient clinical and serological manifestations highly suggestive of systemic lupus erythematosus. in addition, prolonged clinical and serologica ...19947932438
the transcriptional regulator yy1 binds to the 5'-terminal region of b19 parvovirus and regulates p6 promoter activity.we performed a systematic study to identify cellular factors that bound to the terminal repeat region of b19 parvovirus. using electrophoretic mobility shift assays, we detected one cellular factor which prominently bound to the repeat region. the factor was purified from k562 nuclear extract by specific dna affinity column chromatography and identified as yy1, a multifunctional transcription factor. of multiple possible yy1 binding sites in the upstream region of the p6 promoter, three showed s ...19947933098
immune-mediated inner ear disease and parvovirus b19.human parvovirus b19 (hp-b19), the etiologic agent of the common childhood illness erythema infectiosum, has been implicated in systemic immune disorders. patients presenting with sensorineural hearing loss and/or dizziness, not readily categorized, were evaluated for immune-mediated inner ear disease. appropriate serologic studies including parvovirus b19 antibody titers were conducted. thirty patients with suspected immune-mediated inner ear disease were treated with corticosteroid trial for 5 ...19947934594
immunoelectron microscopic identification of human parvovirus b19.a mildly macerated, hydropic fetus was delivered spontaneously at 25 weeks gestation by a multigravidous black mother. at autopsy, gross and microscopic evidence suggested fetal anemia, and excessive extramedullary erythroblastosis was present generally. erythroid precursor cells in the pulmonary capillary circulation contained eosinophilic nuclear inclusions consistent with human parvovirus b19 infection. transmission electron microscopy on osmicated epon lung sections showed lucent, granular c ...19947941039
human parvovirus b19-induced aplastic crisis in iron deficiency anemia.human parvovirus b19 (hpvb19) infects and replicates in erythroid progenitor cells. its specific cytotoxic effect on these cells results in aplastic crises in patients with congenital hemolytic anemias. aplastic crisis due to hpvb19 infection in a healthy girl revealed occult iron deficiency anemia. the condition is characterized by a high serum iron level in the aplastic phase and rapid recovery after administration of iron. temporary hpvb19-induced red blood cell aplasia could occur in patient ...19947942015
[viral infection and fibromyalgia].fibromyalgia (fm) is a very frequent syndrome of unknown cause, characterized by generalized pain, fatigue and a number of tender points to palpation. among the several etiopathogenic hypotheses discussed, the association of fm with some viral infections has been the object of multiple studies due to its relation and similarity with the chronic fatigue syndrome, acknowledges as being related, although not exclusively, with the chronic infection by the epstein-barr virus. many individual descript ...19947942134
acute hepatic sequestration caused by parvovirus b19 infection in a patient with sickle cell anemia. 19947942800
pure red cell aplasia, peripheral thrombocytopenic purpura, and parvovirus b19 infection in a women seropositive for human immunodeficiency virus. 19947948543
[parvovirus b19 and pediatric pathology].b 19 parvovirus is a widespread virus with primary infestation generally occurring in childhood through family and community outbreaks. its most typical manifestation is transient erythroblastopenia with aplastic crisis, often profound, mostly affecting patients with chronic hemolytic anemia, and eventually patients with defective erythropoiesis (chronic hypoplastic anemia, iron deficiency anemia). in normal individuals the primary infestation is usually asymptomatic but may give transient hemat ...19947951839
increased prevalence of igm antibodies to epstein-barr virus and parvovirus b19 in blood donations with above-normal neopterin. 19947955387
viral-associated haemophagocytosis and elevated serum tnf-alpha with parvovirus-b19-related pancytopenia in patients with hereditary spherocytosis. 19947955926
[two cases of human parvovirus b19 infection]. 19947964028
the frequency of human parvovirus b19 infection in nonimmune hydrops fetalis.intrauterine infection of human parvovirus b19 has recently been identified as an etiology for nonimmune hydrops fetalis (nihf) and fetal death. to examine the frequency of b19 infection in cases of nihf, forty two cases of nihf of unknown cause were tested for the presence of b19 antibodies by enzyme-linked immunosorbent assay (elisa) and b19 dna by polymerase chain reaction (pcr). three maternal sera were positive for b19 igm antibody and one fetal serum was positive for b19 dna. overall, four ...19947965545
a putative nucleoside triphosphate-binding domain in the nonstructural protein of b19 parvovirus is required for cytotoxicity.cytotoxicity secondary to b19 parvovirus infection is due to expression of the viral nonstructural protein. nonstructural proteins of many parvoviruses contain a well-conserved nucleoside triphosphate (ntp)-binding motif, which has been shown to be essential for a variety of protein functions. we show here that cytotoxicity of the b19 parvovirus nonstructural protein was abolished by single mutations of amino acids within the ntp-binding domain, especially within the a motif, implicating ntp-bin ...19947966641
immunochemiluminescent southern blot assay for polymerase chain reaction detection of human parvovirus b19 dna.human parvovirus b19 is not only an acute self-limited infection causing erythema infectiosum, transient aplastic crisis, foetal hydrops and arthritis but can also be a chronic infection causing chronic anaemia and associated with chronic neuropathy and vasculitis. serologic studies have proven to be the most sensitive way to detect acute infection in the immunologically normal patient while polymerase chain reaction (pcr) assays for b19 dna are the most sensitive way to detect chronic infection ...19947969192
[acute febrile low back pain revealing parvovirus b19 infection]. 19947971841
childhood autoimmune neutropenia and human parvovirus b19. 19947977312
childhood idiopathic thrombocytopenic purpura: association with human parvovirus b19 infection.infection with human parvovirus b19 is the most common cause of transient aplastic crisis in patients with chronic hemolytic anemia. multiple reports of children with simultaneous b19 infection and thrombocytopenia as well as the known association between experimental b19 infection and thrombocytopenia prompted us to hypothesize that b19 may be associated with childhood idiopathic, or immune, thrombocytopenic purpura (itp). because there is a paucity of evidence regarding a viral etiology for it ...19947978048
chronic parvovirus infection mimicking myelodysplastic syndrome in a child with subclinical immunodeficiency.we present a report of a child with subclinical immunodeficiency who became chronically infected with parvovirus resulting in pancytopenia and morphologic abnormalities in the bone marrow mimicking myelodysplastic syndrome (mds).19947978051
wegener's granulomatosis and parvovirus b19 infection. 19947980681
[laboratory diagnosis of parvovirus b 19 infection: comparison of ten commercial igg- and igm-antibody tests].seven elisas, two westernblots and one immunofluorescence assay for serological detection of parvovirus-b19-infection were compared with regard to their sensitivity and specificity. all ten assays are commercially available and use recombinant proteins (vp1, vp2) from bacterial and eukaryotic expression systems and synthetic viral peptides as antigens. the elisa assays comprise indirect elisa and mu-capture systems. all assays were tested for their sensitivity with follow-up sera from 21 patient ...19947982714
[parvovirus b19 infection: variable clinical presentations]. 19947983422
persistent anaemia in hiv-infected individuals due to parvovirus b19 infection. 19947986425
parvovirus b19 as a cause of acquired chronic pure red cell aplasia.parvovirus b19 infection causes chronic anaemia in immunodeficient individuals by selective suppression of erythropoiesis. the bone marrow morphology is characteristic of pure red cell aplasia (prca). to determine the frequency of b19-induced prca we retrospectively analysed a series of 57 prca patients. b19 dna was present in serum of eight patients (14%) and could be extracted from bone marrow aspirate slides from five of these patients. recent exposure to the virus was confirmed by the presen ...19947986722
application to immunoglobulin m capture hemadherence assays of hemagglutination of monkey erythrocytes by native and recombinant human parvovirus b19 antigens.human parvovirus b19 recently was shown to agglutinate baboon and human erythrocytes. we have now demonstrated that both recombinant and native b19 antigens agglutinate rhesus, cynomolgus, and saimiri monkey erythrocytes. using cynomolgus erythrocytes and the recombinant antigen, we developed an immunoglobulin m (igm) antibody capture hemadherence test (machat) for the detection of specific b19 igm antibodies in human sera. the results obtained with machat were compared with those obtained with ...19947989556
expression of p antigen in parvovirus b19-infected bone marrow. 19957990901
[subacute thyroiditis in parvovirus b19 infection].in a 32-year-old women with clinical and scintigraphic signs of thyroiditis, serological screening showed positive igg and igm titres against parvovirus b19 (pb19). subacute thyroiditis has not previously been reported following pb19 infection. the present case suggests that subacute thyroiditis may be caused by pb19 infection. further screening is necessary to establish whether this infection is more frequently associated with subacute thyroiditis than previously thought.19947992447
low prevalence of antibody to human parvovirus b19 in singapore.a seroepidemiological survey was conducted in singapore to assess the prevalence of antibody to human parvovirus b19. sera were collected from 600 healthy individuals between 6 months and over 50 years of age and tested for igg antibody against b19 virus by antigen capture indirect immunosorbent assay. the overall seropositivity rate was 16.2%. all the children under 5 years of age were seronegative. antibody prevalence increased gradually from 3.5% in school children (5-14 years of age) to 7.7% ...19947995362
[infectious arthritis?]. 19947997304
[blood group p antigen is the cellular receptor for human parvovirus b 19]. 19947998364
[transmission of parvovirus b19 by blood and blood components].to determine the clinical significance of parvovirus b19 (b19) and the potential risk of transmission by blood or plasma products. procedures to avoid this transmission are discussed. data sources and selection criteria: known physico-chemical data of autonomous parvoviridae, and studies on the incidence of b19 in blood or plasma products, were considered. data on b19 infection published by the author and/or other investigators.19948000251
[possibilities of virus inactivation of pooled fresh plasma with tri-n-butylphosphate (tnbp) detergents (sd procedure)].treatment with tri-n-butyl-phosphate and detergent (sd-treatment) leads to efficient inactivation of viruses having a lipid enveloped surface, like hepatitis b virus, hepatitis c virus and human immunodeficiency virus, that are presently the most transfusion relevant viruses in germany. other lipid enveloped viruses of the herpes group like cytomegalovirus and epstein-barr virus are inactivated as well. non-enveloped viruses like parvovirus b19 and picornaviruses are not inactivated by sd-treatm ...19948000260
[joint manifestations and parvovorus b19 infections: 8 cases].human parvovirus b19 (pb19) may cause joint manifestations. we report eight cases of polyarthritis associated with pb19 igm: six cases of acute polyarthritis spontaneously resolving within a few days, and two other cases suggesting that pb19 should be responsible for accutisation of chronic disease (lupus with kikuchi's disease and hla b27 related arthritis).19938009016
[human infection with parvovirus b19]. 19948011716
clinical virology in children.virology has become an increasingly important field of medicine in the past decade. many infectious diseases that were once of unknown etiology can now be linked to the specific viral pathogen. new methods for diagnosing and treating viral diseases are being developed. in addition, antiviral therapy or prophylaxis is available for many of these viral illnesses. this article reviews the basic medical aspects of some viral infections. human parvovirus b19 causes erythema infectiosum, with the clas ...19948017260
clinical characteristics of children with hereditary hemolytic anemias and aplastic crisis: a 7-year review.we reviewed the clinical courses of children < or = 16 years of age with hereditary hemolytic anemias who were admitted to the university of mississippi medical center for aplastic crisis. constitutional signs, gastrointestinal complaints, and headache were the most frequent findings. statistically significant decreases in hemoglobin levels, hematocrit, and reticulocyte counts, but not total white blood cell or platelet counts, were found on admission when compared to other times of determinatio ...19948023203
[human parvovirus b19 (hpv-b19), a clinical overview with emphasis of rheumatologic manifestations].the human parvovirus b19 (hpv-b19) causes a spectrum of diseases: erythema infectiosum (fifth disease), aplastic crises in patients with chronic hemolytic anemias, chronic bone marrow failure in patients with congenital or acquired immune deficiency, vascular purpura, hydrops fetalis, abortion, intrauterine fetal death and hpv-19-arthropathy. in this review we will discuss the clinical pattern, the diagnostic questions and therapeutic possibilities particularly relating to rheumatic manifestatio ...19948023586
the structure of human parvovirus b19 at 8 a resolution.empty capsids of the human parvovirus b19, self-assembled in a baculovirus expression system, have been crystallized in a cubic space group p2(1)3 with a = 362 a. in spite of extensive purifications, the crystals diffract x-rays to only 8.0 a resolution. diffraction data were collected using oscillation photography with synchrotron radiation. the orientations of the particles in the unit cell were determined with a self-rotation function and their positions were obtained with an r-factor search ...19948030266
severe pneumonia after heart transplantation as a result of human parvovirus b19.the diverse manifestations of human parvovirus b19 infection have been well established. erythema infectiosum, fetal hydrops, adult arthropathy, and aplastic anemia in patients with hemoglobinopathies or underlying immunocompromise have been described. recently we successfully treated a patient who, after heart transplantation, had fever, rash, and pneumonia with respiratory failure caused by human parovirus b19. human parovirus b19 has not been reported previously as a pathogen causing pulmonar ...19948031818
does intravenous immune globulin have a role in hiv-infected patients?the main immunological abnormality in human immunodeficiency virus (hiv)-infected patients, and particularly those with the acquired immune deficiency syndrome (aids), is a deficiency in cellular immunity. however, symptomatic hiv-infected children also have evidence of deficiency of specific antibody synthesis, and intravenous immune globulin (ivig) preparations in doses of 0.2-0.4 g/kg every 2-4 weeks have been shown to reduce the incidence of respiratory infections. ivig therapy may also redu ...19948033437
viral safety of factor viii concentrates. 19948036799
persistent erythema infectiosum-like rash as a prodrome of acute lymphocytic leukemia.a 7-year-old boy had erythema infectiosum with typical manifestations. over more than 40 days, these lesions showed no sign of regression, and chronic anemia became progressively more severe. electron microscopic examination performed 20 days after onset showed abnormal sezary-like lymphocytes. bone marrow biopsy, which was performed to evaluate the anemia, was consistent with acute lymphocytic leukemia. persistent parvovirus b19 infection may be connected with immunosuppression. therefore, earl ...19948041657
erythema infectiosum and other parvovirus b19 infections.the human parvovirus b19 has been identified as the causative agent in several diseases, including the benign childhood condition erythema infectiosum (fifth disease), a polyarthropathy that occurs in adults, aplastic crisis and hydrops fetalis. the virus has also been associated with numerous other hematologic, neurologic and rheumatic conditions. except for the potential of neonatal death due to fetal hydrops, the prognosis of patients with parvovirus infection is generally good. the diagnosis ...19948042568
a fetus with a parvovirus b19 infection and congenital anomalies.a fetus with multiple structural defects was seen at prenatal ultrasound examination. after termination of the pregnancy a bilateral cleft lip, alveolus, and palate; micrognathia; and webbed joints were seen. fetal tissues showed indications of infection, intranuclear inclusion bodies, chronic stress, haemolysis, arterial wall damage, and profuse haemorrhage. parvovirus b19 dna was detected in fetal tissues by dot hybridization after polymerase chain reaction. the possibility of parvovirus b19 i ...19948052564
human parvovirus b19 infection and unbalanced translocation in a case of hydrops fetalis.in a case of hydrops fetalis, serological examination showed a recent maternal human parvovirus b19 infection. amniocentesis revealed a unique unbalanced translocation between chromosomes 3 and 11 of the fetus. the mother proved to have a balanced reciprocal translocation between chromosomes 3 and 11. a grossly macerated hydropic male fetus was delivered with a flat nose and low implanted deformed ears. histopathological examination revealed nuclear inclusion bodies in fetal erythroid cells, con ...19948052566
[purpuric eruption and cheilitis secondary to parvovirus b19 infection]. 19938053780
a recombinant immunoblot and elisa for detection of acute parvovirus b19 infection.laboratory diagnosis of parvovirus b19 (b19) infection has been hampered by the limited availability of b19 virus. recombinant viral proteins are now available for use as antigen in serological assays. we compared detection of anti-b19 igm by "mu-capture assay" using viral b19 particles to a recombinant (rec.) immunoblot and a rec. enzyme-immunoassay (elisa) using viral structural proteins as antigens expressed in e. coli. the rec. immunoblot was 94.3% sensitive and 96.4% specific for anti-b19 i ...19948061414
[parvovirus b19-induced aplastic crisis in a patient with iron deficiency anemia].a 38-year-old female was referred to takaoka city hospital for treatment of common-cold-like symptom and an episode of transient unconsciousness. physical examination on admission revealed severe anemia and an ejection heart murmur. complete blood count revealed microcytic hypochromic anemia (hb 4.1 g/dl), leukocytopenia (2.600/microliters), thrombocytopenia (7.1 x 10(4)/microliters) and reticulocytopenia (17,000/microliters). the bone marrow cellularity was within normal limits. cells in the er ...19948065019
conjunctivitis caused by human parvovirus b19 infection.a 26-year-old woman with erythema infectiosum is reported. she had small faint erythemas on her extremities and congestion of palpebral and bulbar conjunctiva of both eyes. human parvovirus b19 (b19) dna was detected in the specimen of the conjunctiva by the polymerase chain reaction. this result suggests that conjunctivitis in erythema infectiosum may cause the b19 infection.19948065702
viral infection including hiv and aids.viruses have long been considered candidates for infectious etiologic agents or cofactors in the development of rheumatic diseases. the current epidemic of hiv infection and the recognition of "emerging viruses" has focused interest on the possible role of viruses in pathogenesis of diseases without defined etiology. over the past year, the role of parvovirus b19 in chronic arthropathy was further defined. additional data added to our understanding of the mechanisms by which rubella virus may ca ...19948068514
molecular, cellular and clinical aspects of parvovirus b19 infection. 19948074799
henoch-schönlein purpura and human parvovirus b19. 19948075461
parvovirus b19-associated red blood cell aplasia in combined immunodeficiency with normal immunoglobulins. 19948078745
parvovirus particles as platforms for protein presentation.empty capsids of the human pathogenic parvovirus b19 can be produced in a baculovirus system. b19 capsids are composed mainly of major capsid protein (vp2) and a small amount of minor capsid protein (vp1); vp1 is identical to vp2 but contains an additional 227-aa n-terminal region ("unique" region). a portion of that region of vp1 is external to the capsid, and vp1 is not required for capsid formation. we substituted the unique region with a sequence encoding the 147 aa of hen egg white lysozyme ...19948078912
case report: detection of parvovirus b19 in a skin biopsy of a patient with erythema infectiosum.we report the findings on a skin biopsy taken from a child acutely infected with parvovirus b19 showing the typical exanthematous rash. by indirect immunofluorescence with a monoclonal antibody to b19, viral capsid proteins were detected in epidermal cells localized mainly in the stratum basale. additionally, b19 dna was detected in epidermal cells of the stratum basale by in situ hybridization using a dig-labelled b19 dna probe. the detection of viral capsid proteins and viral dna suggests the ...19948083666
[infectious arthritis?]. 19948090228
on the lack of evidence of parvovirus b19 in acute obstructive airway disease. 19948090615
should measures be taken to reduce the risk of human parvovirus (b19) infection by transfusion of blood components and clotting factor concentrates? 19948091460
human parvoviruses: implications for transfusion medicine. 19948091474
distribution of hepatitis a antibody over a process for the preparation of a high-purity factor viii concentrate.the levels of hepatitis a antibody were studied in factor viii products manufactured by the scottish national blood transfusion service. it was found that the current high-purity factor viii product has no detectable hepatitis a antibody, whereas the superseded intermediate-purity product had significant antibody levels (6,700 miu/ml). although the finished high-potency factor viii product has no detectable hepatitis a antibody, significant levels of antibody are found in the early stages of pro ...19948091738
parvovirus b19 infection in pregnancy. 19948092869
persistent arthritis after human parvovirus b19 infection. 19938094512
human parvovirus infection in homozygous sickle cell disease.we studied the epidemiology of human parvovirus b19 infection in 308 children with homozygous sickle cell (ss) disease and 239 controls with a normal haemoglobin (aa) genotype followed from birth in a cohort study. annual serum samples identified the time and frequency of b19 infection, which did not differ between ss and aa children, about 40% of each group developing specific igg by age 15. b19 infection followed an epidemic pattern similar to that observed for aplastic crises; accounted for a ...19938098391
human parvovirus b19 and arthritis. 19938098419
adult still's disease associated with acute human parvovirus b19 infection. 19938098420
heart failure after parvovirus b19 infection. 19938098810
neuralgic amyotrophy after parvovirus b19 infection. 19938102466
parvovirus b19 antibodies in sera of patients with unexplained exanthemata from belém, pará, brazil. 19938107612
b19 parvovirus infection in children with malignant solid tumors receiving chemotherapy.two children with rhabdomyosarcoma developed severe anemia following chemotherapy; anemia was more severe compared to that observed following earlier chemotherapy cycles. while one patient had a brisk reticulocytosis, the other had no demonstrable reticulocytes. both patients had evidence of acute b19 parovirus infection and subsequently developed appropriate antibody response. a diagnosis of b19 parvovirus infection should be considered in any patient who develops persistent or severe anemia wh ...19948107656
human parvovirus b19 encephalopathy. 19948110015
in search of intraocular antibody production to parvo b19 virus and adenovirus in intermediate uveitis. 19938110689
evaluation of strand-specific rna probes visualized by colorimetric and chemiluminescent reactions for the detection of b19 parvovirus dna.a dot-blot hybridization assay was developed to detect b19 dna using strand-specific rna probes labelled with digoxigenin. the sensitivity of the assays was evaluated either using 'plus' and 'minus' sense rna probes in two different hybridization assays, or in two successive reactions of the same assay. the hybridized probes were revealed immunoenzymatically using anti-digoxigenin fab fragments conjugated with alkaline phosphatase. the enzyme was visualized by colorimetric reaction. since 'minus ...19938113343
[detection of parvovirus b19 in the blood of patients using dna hybridization].dna assay was used in detection of parvovirus b19. the sera of 63 patients were examined and the presence of dna virus was found in two samples.19938128645
does parvovirus b19 have a role in rheumatoid arthritis?to determine whether parvovirus b19 (b19) infection is associated with rheumatoid arthritis (ra).19948129454
influence of previous exposure to human parvovirus b19 infection in explaining susceptibility to rheumatoid arthritis: an analysis of disease discordant twin pairs.to assess the association between exposure to parvovirus b19 and susceptibility to rheumatoid arthritis (ra).19948129459
parvovirus b19 as a cause of anemia in human immunodeficiency virus-infected patients. 19948133116
parvovirus b19 induced red cell aplasia in myelofibrosis.a 38-year-old female presented with moderate anaemia and a leucoerythroblastic blood film. subsequent investigation showed myelofibrosis in cellular phase. her haemoglobin quickly and spontaneously recovered with concurrent serological evidence of recent parvovirus b19 infection. this is the first report in the literature of parvovirus causing red cell aplasia in myelofibrosis.19938136287
parvovirus b19-related lymphadenopathy.we tested whether parvovirus b19 (pvb19) is involved in benign lymph node swelling as a causative agent. the serum specimens from 13 patients with lymph node swelling of unidentified cause were tested for igm and igg antibody against pvb19 by western blot analysis. five patients had igm antibody, and pvb19 dna was detected in the serum from one of them by dot-blot analysis, suggesting the recent pvb19 infection and its involvement in lymph node swelling of these patients. clinical and haematolog ...19938136290
primary infection by human parvovirus b19. 19948136549
papular-purpuric 'gloves and socks' syndrome: not only parvovirus b19.we previously described an acute dermatosis characterized by pruritic erythematous and slightly papular lesions on the hands and feet in a 'gloves and socks' distribution associated with oral aphthoid lesions and fever (papular-purpuric 'gloves and socks' syndrome = ppgss). we strongly suspected a viral origin, but serologic tests for a large panel of viruses remained negative. subsequently, 2 cases of ppgss with serologic evidence of a parvovirus b19 infection have been reported in the literatu ...19948136553
resistance to parvovirus b19 infection due to lack of virus receptor (erythrocyte p antigen).the presence of a specific cellular receptor is thought to be necessary for susceptibility to viral infection. the erythrocyte p antigen is the cellular receptor for parvovirus b19. we hypothesized that the rare persons with the p phenotype, whose erythrocytes do not have this receptor, would be naturally resistant to b19 infection, which causes erythema infectiosum.19948139629
[erythroid aplasia caused by parvovirus b19 in congenital dyserythropoietic anemia]. 19938140511
[bullous erythema multiforme caused by human parvovirus b19]. 19938142517
microplate capture hybridization of amplified parvovirus b19 dna fragment labelled with digoxigenin.a capture hybridization technique in microplate has been developed for the identification of polymerase chain reaction (pcr) amplified b19 dna fragment in clinical specimens. the amplified 104 bp b19 dna fragment, located in the gene coding for structural proteins, was directly labelled during the amplification reaction by incorporation of digoxigenin-labelled dutp. the amplified product was then captured by a probe immobilized on microplate wells. the capture hybridization reaction was visualiz ...19938145776
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