| [molecular biological bases for adenovirus-induced syntheses in cells]. | the latest data from literature concerning the peculiarities of the molecular structure of adenoviruseses and their reproduction in a cell are summarized. analysis of experimental materials gives evidence that the regulation of adenovirus-induced syntheses in the infected cell takes place at the transcription and translation level; adenoviruses themselves can be used as a model for studying molecular mechanisms for reproduction of dna-containing viruses. | 1976 | 936295 |
| characterization of host-range and temperature-sensitive mutants of adenovirus type 5 with particular regard to transformation of a hamster embryo cell line (nil). | | 1976 | 944968 |
| [use of organ cultures of animal trachea and lung for studying respiratory viruses]. | organ cultures of the trachea and the lungs of newborn guinea pigs, rabbits, chickens, cow and pig embryos supported multiplication of influenza a2 and adenovirus type 3 viruses. trachea organ cultures were shown to be more sensitive to the viruses under study. organ cultures of the chicken trachea can be used for diagnosis of respitaroy virus infections. | 1976 | 945643 |
| application of a microtiter cell-culture method to characterization of avian adenoviruses. | a microtiter cell-culture method was developed and used to titrate virus isolates for characterization. virus dilutions and chicken kidney cell suspensions were dispensed into the wells of disposable microculture plates, with infectivity endpoints being determined microscopically on the fifth or sixth day, or by reading crystal-violet-stained monolayers on day 6. with this method, 37 candidate avian adenoviruses isolated from diagnostic accessions were characterized as avian adenoviruses (aav). ... | 1976 | 945730 |
| an assessment of the generation times of neuronal precursors in the sensory retina and subventricular zone of the newborn rat. | generation times and the times for their respective phases were determined for neuronal precursors in the sensory retina and subventricular zone of the brain of newborn cd rats. these regions can be induced to form retinoblastoma and medulloepitheliomas, respectively, following injection of adenovirus-12. through use of autoradiographic techniques, it was shown that the cell cycle duration in the subventricular zone was almost twice as long as in the sensory retinal layer. the g1 phase durations ... | 1976 | 949631 |
| location and identification of the genes for adenovirus type 2 early polypeptides. | virus-specific rna was prepared from cells early after adenovirus type 2 infection and fractionated by hybridization to specific fragments of viral dna. the viral mrna was used to program cell-free protein synthesis, and the products were analyzed by electrophoresis. the genes for the early polypeptides of apparent molecular weight 44,000, 15,000, 72,000, 15,500, 19,000, and 11,000 daltons were located, respectively, between positions 0-4.1, 4.1-16.7, 58.5-70.7, 75.9-83.4, 89.7-98.6, and 89.7-98 ... | 1976 | 949741 |
| adenovirus protein maturation at 42 degrees c. | incubation of adenovirus type 2 infected cells at 42 degrees c resulted in an inhibition of assembly of virus particles although all the major viral structural polypeptides and virus-induced cellular polypeptides so far identified were detected by electrophoretic analysis. selective high salt-acid-urea extraction of low mol. wt. polypeptides revealed the absence of protein vii at 42 degrees c whereas precursor polypeptide p-vii and core protein v were found. pulse-chase and temperature shift exp ... | 1976 | 950560 |
| detection of adenovirus type 2-induced early polypeptides using cycloheximide pretreatment to enhance viral protein synthesis. | (35s) methionine-labeled polypeptides synthesized by adenovirus type 2-infected cells have been analyzed by polyacrylamide gradient gel electrophoresis and autoradiography. cycloheximide (ch) was added to infected cultures to accumulate early viral mrna relative to host cell mrna. this allowed viral proteins to be synthesized in increased amounts relative to host proteins after removal of ch and pulse-labeling with (35s)methionine. during the labeling period arabinosyl cytosine was added to prev ... | 1976 | 950686 |
| adenovirus type 12-specific rna sequences during productive infection of kb cells. | the complementary strands of adenovirus type 12 dna were separated, and virus-specific rna was analyzed by saturation hybridization in solution. late during infection whole cell rna hybridized to 75% of the light (1) strand and 15% of the heavy (h) strand, whereas cytoplasmic rna hybridized to 65% of the 1 strand and 15% of the h strand. late nuclear rna hybridized to about 90% of the 1 strand and at least 36% of the h strand. double-stranded rna was isolated from infected cells late after infec ... | 1976 | 950688 |
| isolation and characterization of temperature-sensitive mutants of adenovirus type2. | fourteen temperature-sensitive mutants of human adenovirus type2, which differed in their plaquing efficiencies at at the permissive and nonpermissive temperatures by 4 to 5 orders of magnitude, were isolated. these mutants, which could be assigned to seven complementation groups, were tested for their capacity to synthesize adenovirus dna at the nonpermissive temperature. three mutants in three different complementation groups proved deficient in viral dna synthesis. the dna-negative mutant h2t ... | 1976 | 950689 |
| characterization of adenovirus type 2 transcriptional complexes isolated from infected hela cell nuclei. | hela cell nuclei, isolated 17 h after infection with human adenovirus type 2 (ad2), were treated with 200 mm ammonium sulfate. the extract (s200 fraction) contained 50 to 70% of the nonintegrated ad2 dna, which was in the form of nucleoprotein complexes. these complexes contained native, intact ad2 dna (with the exception of replicative intermediates) and could be partially purified and resolved by velocity gradient centrifugation. using high-salt (200 mm ammonium sulfate) incubation conditions, ... | 1976 | 950690 |
| [adenovirus typing and distribution in swine]. | adenoviruses isolated from pigs (ro-kl; sa3; ro; and r10) and the prototypic strain compton 25r, belonging to the 1st serologic group, are responsible for the formation of intranuclear bodies of the cowdry-a type, while the virus ad5 strain (isolated from humans) cause the formation of another variant of nuclear inclusions of the cowdry-b type known as central mass. all virus strains propagate in primary cell cultures of pig kidney and calf and rabbit kidney, calf testis, and in the stable cell ... | 1976 | 951929 |
| a new species of virus-coded low molecular weight rna from cells infected with adenovirus type 2. | a virus-coded low molecular weight rna (5.2s), which migrates slightly faster on polyacrylamide gels than the well characterized adenovirus-specific 5.5s rna, has been isolated from cells infected with adenovirus type 2. hybridization-competition experiments and rna fingerprints indicate that the two virus-associated (va) rnas differ in their primary structures. the gene for 5.2s rna is located to the right of the gene for 5.5s rna, on the i strand of a dna segment which extends between position ... | 1976 | 954088 |
| intermediates in adenovirus assembly. | three intermediates in adenovirus assembly have been defined; nuclear intermediates, young virions, and mature virions. the nuclear intermediates are fragile and heterogenous in size (550s-670s) and withstand separation on ficoll gradients but fall apart upon cscl gradient centrifugation unless prefixed with glutaraldehyde. they contain both capsid and core structures, and the core structures are preferentially released during purification in cscl. the precursor polypeptides pvi and pvii are pre ... | 1976 | 957481 |
| characterization of a temperature-sensitive, hexon transport mutant of type 5 adenovirus. | infection of kb cells at 39.5 degrees c with h5ts147, a temperature-sensitive (ts) mutant of type 5 adenovirus, resulted in the cytoplasmic accumulation of hexon antigen; all other virion proteins measured, however, were normally transported into the nucleus. immunofluorescence techniques were used to study the intracellular location of viral proteins. genetic studies revealed that h5ts147 was the single member of a nonoverlapping complementation group and occupied a unique locus on the adenovir ... | 1976 | 957484 |
| susceptibility of teratocarcinoma cells to adenovirus type 2. | | 1976 | 958395 |
| intracellular distribution and polyadenylate content of adeno-associated virus rna sequences. | | 1976 | 960561 |
| pharyngeal isolations of adenovirus 31 from a family population. | during a 14-month period in 1965 and 1966, adenovirus type 31 was isolated from 26 pharyngeal swabs obtained on a routine schedule from children and adults during a study of respiratory viruses among members of a family population. eighteen isolations were made in a four-day period, seven within the preceding five months, and the last, eight months earlier. a significant association between the isolation of adenovirus 31 and respiratory or gastrointestinal illnesses was not demonstrable. limited ... | 1976 | 961693 |
| comparison of replication of adenovirus type 2 and type 4 in human lymphocyte cultures. | adenovirus type 2 was capable of replicating in purified lymphocyte cultures from human adenoid specimens. phytohemagglutinin stimulation enhanced the replication of virus. viral titers of 103 to 104 50% tissue culture infective doses per ml were reached after 4 to 8 days. only 1 to 3 per 106 cells were found to produce virus. in contrast, there was no evidence that lymphocyte cultures could support the replication of adenovirus type 4. the life span of cultures infected with type 2 or 4 was not ... | 1976 | 965085 |
| two complementary strand-specific termination sites for adenovirus dna replication. | adenovirus type 2 dna, specifically labeled at the termini for dna replication, was prepared by isolation of viral dna molecules which were completed during short pulses with 3h-thymidine. the distribution of radioactivity in the two complementary strands at the termini for dna replication was determined by liquid phase hybridization and gelelectrophoresis. at the right-hand terminus, nearly all radioactivity was found in the viral h strand, whereas at the left-hand terminus, most radioactivity ... | 1976 | 975246 |
| oncogenic dna viruses--replication, tumor gene expression, and role in human cancer. | | 1976 | 982080 |
| factors regulating cellular dna synthesis induced by adenovirus infection. ii. the effects of actinomycin d on productive virus-cell systems. | | 1976 | 982824 |
| studies on synthesis and structure of virus-coded low molecular weight rna in adenovirus type 2-infected kb cells. | | 1976 | 982833 |
| [characteristics of several indices of the mitotic regime of clone strains of hela cells infected with adenovirus type 7]. | the effect of adenovirus type 7 on the mitotic activity, the level and qualitative characteristics of pathological mitoses was studied in mycoplasma-free and latently mycoplasma-infected cells of clone hela cell lines with different sensitivity to this virus. the level and the extent of changes of the mitotic regimen in adenovirus-infected cells depend upon their susceptibility to the virus and initial characteristics of the mitotic regimen of the culture which is somehow related to the presence ... | 1976 | 983002 |
| isolation and characterization of adeno-associated viruses from bovine adenovirus types 1 and 2. | hemagglutinating dna viruses of 20 nm diameter were isolated from bovine adenovirus types 1 and 2. the isolates were heat stable, chloroform resistant, and defective. their densities were 1.38 to 1.39 g/cm3, and they were found to be serologically identical to the bovine adeno-associated virus strain x7. a partial antigenic relationship was found between these and the canine adeno-associated virus. | 1976 | 986122 |
| [the fate of corneal infiltrations in cases of epidemic keratoconjunctivitis. a follow-up study over two and a half years (author's transl)]. | 34 out of 72 patients (47.2%) with epidemic keratoconjunctivitis due to an infection with the adeno-virus type 8 still showed, on average, corneal opacities after 2.3 years. the gamma2-test proved the statistical significance of the correlation between the prevention of persistent corneal changes and the abstention from topical treatment with corticosteroids in the acute phase of the disease. yet, steroids mitigate the subjective discomfort, delay the development of keratitis by several days, or ... | 1976 | 997526 |
| [electron microscopic studies of cell cultures of various origins]. | thirty-four cell cultures of various origins were examined in the electron microscope. mycoplasma were found in 31 cultures, a, c and d particles of oncornaviruses in 27 cultures, adenovirus in one culture and paramyxovirus in 8 cultures. two cultures were found to be virus- and mycoplasma-free. a stringent control of cell cultures for the presence of viruses, mycoplasma or other microbiological agents is recommended. | 1976 | 1007223 |
| a comparison of nuclear and cytoplasmic viral rnas synthesized early in productive infection with adenovirus 2. | | 1976 | 1019348 |
| [proliferation of a culture of rat fibroblasts chronically infected with oncogenic a-12 virus]. | | 1976 | 1034369 |
| studies on the new cell line tt-i: viral susceptibility and chromosomal changes related to mycoplasma contamination. | for understanding some biological properties of tt-1 cell line established in our laboratory in 1962, its viral susceptibility and the changes in chromosomes related to mycoplasma contamination were studied. tt-1 cells were susceptible to the infection of picorna (polio type 1, coxsackie b1 and echo type 2), measles, newcastle disease, herpes simplex (type 1), adeno (type 12) and vaccinia viruses. contamination of tt-1 cells with mycoplasma hominis was treated successfully with bottromycin a2 (5 ... | 1976 | 1037533 |
| involvement of the microtubule in replication cycles of animal viruses. | | 1975 | 1056754 |
| cell transformation: a study of regulation with types 5 and 12 adenovirus temperature-sensitive mutants. | | 1975 | 1057471 |
| genetic analysis of human adenovirus type 5 in permissive and nonpermissive cells. | | 1975 | 1057472 |
| mapping of adenovirus 2 rna sequences in lytically infected cells and transformed cell lines. | the strands of the six ecori fragments and the hpai fragments e and c of ad2 dna were separated by electrophoresis in agarose gels. using 32p-labeled fragment strands in solution hybridization experiments, the fraction of each strand complementary to rna extracted from infected or transformed cells was assayed by chromatography on hydroxylapatite. in this manner, a tentative map of the cytoplasmic rna sequences has been constructed for viral rna extracted from cells both early and late during in ... | 1975 | 1057474 |
| an examination of the nuclear rna of adenovirus-transformed cells. | | 1975 | 1057475 |
| rna transcription in cultures productively infected with adenovirus 2. | | 1975 | 1057476 |
| the transcriptional role of host dna-dependent rna polymerases in adenovirus-infected kb cells. | from the present experiments, we can conclude that late in infection two classes of dna-dependent rna polymerase transcribe the viral genome. although we cannot definitively exclude the possibility that one or both of these enzymes are coded for by the virus, it appears more probable that these are hos enzymes, because of the coincidence of the alpha-amanitin sensitivities for synthesis of specific viral rnas with the toxin sensitivities of the respective host rna polymerases. furthermore, the c ... | 1975 | 1057477 |
| adenovirus-2 mrna is transcribed as part of a high-molecular-weight precursor rna. | the order of transcription and the length of nascent rna transcripts from adenovirus-2 (ad-2) dna in the nucleus of infected cells has been deduced by labeling the growing rna chains in vivo for a very brief period, separating the rna on the basis of size, and hybridizine to the ordered ecori restriction endonuclease fragments derived from ad-2 dna. the majority of the virus-specific rna molecules are synthesized as very high-molecular-weight units beginning at a common point at least 25-30,000 ... | 1975 | 1060124 |
| chromatin-like organization of the adenovirus chromosome. | staphylococcal nuclease (nucleate 3'-oligonucleotidohydrolase; ec 3.1.4.7) cleaved dna within disrupted adenovirus particles into a regular series of fragments with a repeat unit of 200 base pairs. since this pattern did not eppear when dna alone was digested, we postulate that the orderly arrangement of core polypeptides protects discrete regions of dna from nuclease attack. the 23 x 10(6) dalton adenovirus dna molecule can accommodate 180 units of roughly 200 base pairs. based on the stoichiom ... | 1976 | 1061143 |
| visualization by immune electron microscopy of viruses associated with acute respiratory disease. | a sensitive and rapid method for detecting adenovirus and influenza viruses has been demonstrated by using both the direct and indirect immune electron microscopy technique. the indirect method was from 4 to over 32 times more sensitive than the direct method. the technique appears to have the sensitivity approaching tissue culture methods, but is much more rapid, requires less laboratory support personnel and space, and should lend itself to detection of noncytologic agents. these data suggest ... | 1975 | 1080174 |
| a study of the oncogenicity of adenovirus type 2 transformed rat embryo cells. | rat embryo cells were transformed by adenovirus type 2 at different multiplicities of infection. the oncogenic potential of the resulting transformed lines varied considerably but a relationship was found to exist between transforming virus dose and oncogenicity of the resulting transformed lines. possible reasons for the considerable variation in oncogenicity of the transformed lines are discussed, giving particular consideration to cellular antigenicity and recent molecular biology studies of ... | 1975 | 1080749 |
| detection of adenovirus in patient specimens by indirect immune electron microscopy. | immune electron microscopic procedures for the detection of adenovirus type 7 directly in throat swab specimens from patients are described. nineteen of 25 throat swab samples, known to be positive for adenovirus type 7 by isolation of virus from tissue culture, were shown to contain aggregates of adenovirions coated with antibody. sensitivity tests of the immune electron microscopic method showed that as few as 16 to 32 tissue culture infective doses of virus could be detected by the direct imm ... | 1975 | 1080765 |
| temperature-sensitive cell mutations that inhibit adenovirus 2 replication. | five temperature-sensitive growth mutants of the hamster cell line bhk-21 were tested for the ability to support adenovirus 2 multiplication at 39 degrees and 33 degrees. wild-type bhk-21 and mutants ts 422e and ts bch yielded comparable amounts of virus at 33 degrees and 39 degrees, whereas in three other mutants, ts t22, ts t23, and ts af8, virus production at 39 degrees was reduced to about 1% of that at 33 degrees. virus yield in the three mutants was not reduced because of a delay in virus ... | 1975 | 1090935 |
| oxime derivatives of erythromycin: inhibitors of rous sarcoma virus reverse transcriptase activity and focus formation. | bovine adenovirus type 3 (bav-3), strain wbr-1, quantitatively transforms hamster embryo carcass cells with a maximum efficiency of 4-7 x 10-4 p.f.u./focus-forming units (f.f.u). this frequency is signigicantly inhibited by the presence of 1-8 mm of ca++. the transformed cells are highly tumourigenic in hamsters and possess t- and tumour-specific transplantation antigens. bav-3 is one of the most oncogenic of the adenoviruses and an ideal model virus for the study of transformation. | 1975 | 1091717 |
| virus assembly. | | 1975 | 1094918 |
| [laboratory procedures in adenoviruses. ii. sensitivity of various cell cultures as determined by endpoint titration (author's transl)]. | the sensitivity of human embryonic kidney (hek), human amnion (ham) and hela cells for human adenoviruses was investigated by means of endpoint titrations of 13 prototype strains and numerous original specimens from patients. prototypes and original specimens showed the same behavior. adenoviruses of subgrouppp iii produced nearly identical titers in all 3 cell cultures, while for subgroup i ham cells showed a 10- to 100-fold lower titer. on the other hand, ham cells are nearly as sensitive as h ... | 1975 | 1098328 |
| translation of viral rna in cell-free systems from eukaryotes. | | 1975 | 1098855 |
| effect of acetone fixation on infectivity and antigenicity of respiratory syncytial virus and adenovirus in the fluorescent antibody test. | an investigation was made on the effect of acetone fixation on infectivity and immunofluorescent antigenicity of respiratory syncytial virus and adenovirus type 5, lipid- and nonlipid-containing viruses, respectively. viruses were allowed to replicate in hep-2 cells, and the cells were then fixed in acetone at 5 c for periods ranging from 30 s to 7 days. treatment for 10 min was sufficient to inactivate respiratory syncytial virus, whereas infectious adenovirus type 5 could be isolated from cell ... | 1975 | 1100650 |
| cleavage of adenovirus messenger rna and of 28s and 18s ribosomal rna by rnase iii. | escherichia coli ribonuclease iii cleaves adenovirus messenger rna and mammalian 28s and 18s ribosomal rna. fragmentation is not random, but in each case a specific collection of products is generated. this points to the potential use of the enzyme as a tool for specific fragmentation of rna. cleavage by rnase iii abolishes the capability of adenovirus messenger rna to direct cell-free synthesis of virus polypeptides. | 1975 | 1103139 |
| the nature of adenovirus persistence in human adenoid vegetations. | ninety-seven adenoid vegetations (av) originating from children aged 2 to 11 years were examined for the presence of adenoviruses. no infectious virus was detected in cell-free homogenates. however, adenovirus was recovered in 30 instances from either fragment or trypsinized cell cultures, or both, of the same tissues. the viruses belonged to types 1, 2, 5 and 6. it was determined by the infectious center assay that the frequency of virus-producing cells in different av varied between 1 of every ... | 1975 | 1106359 |
| studies on the mechanism of replication of adenovirus dna. iv. discontinuous dna chain propagation. | | 1975 | 1111211 |
| genetic analysis of adenovirus type 2. i. isolation and genetic characterization of temperature-sensitive mutants. | temperature-sensitive mutants which replicate normally at 33 c but poorly at 39 c were isolated from nitrosoguanidine- or nitrous acid-mutagenized adenovirus 2 by (i) testing the cytopathic effect or inclusion body-forming capacity of random plaque isolates, or (ii) reduced plaque enlargement upon shifting from 33 to 39 c. thirty-six mutants were isolated with 33 c/39 c plaque ratios varying from 20 to 10-5. some of these mutants could be arranged into 13 groups by the complementation test. by m ... | 1975 | 1113369 |
| sequence relationships between adenovirus 2 early rna and viral rna size classes synthesized at 18 hours after infection. | synthesis of cytoplasmic viral rna was studied during infection of cultured human (kb) cells with adenovirus 2. at 6 h, before viral dna synthesis began 5% of the poly(a)-containing rna hybridized to viral dna; by 12 h and at later times more than 80% was virus specified. at 18 h after infection, four major size classes of cytoplasmic viral rna were identified among the poly(a)-containing molecules. these size classes migrated as 27s, 24s, 19s, and 12 to 15s in polyacrylamide gels. the three lar ... | 1975 | 1113370 |
| specific cleavage of adenovirus-associated virus dna by restriction endonuclease r-ecori--characterization of cleavage products. | | 1975 | 1114700 |
| retinal tumor induction by ocular inoculation of human adneovirus in 3-day-old rats. | a direct causal relationship between a human dna virus, adeno serotype 12, and malignant transformation in target cells (sensory retinal neuronal precursors) was suggested by the development of a remarkably uniform retinoblastoma-like neoplasm in rats. in order to focus upon incipient photoreceptor differentiation, 27 3-day-old cd rats were selected for intraocular virus inoculation. a single injection of 0.03 ml of the virus fluid, 104.5 tcid50 hela cells/0.1 ml was given in the left eye. withi ... | 1975 | 1117320 |
| adenovirus type 2 dna replication. i. evidence for discontinuous dna synthesis. | isolated nuclei from adenovirus type 2-infected hela cells catalyze the incorporation of all four deoxyribonucleoside triphosphates into viral dna. the observed dna synthesis occurs via a transient formation of dna fragments with a sedimentation coefficient of 10s. the fragments are precursors to unit-length viral dna, they are self-complementary to an extent of at least 70%, and they are distributed along most of the viral chromosome. in addition, accumulation of 10s dna fragments is observed e ... | 1975 | 1117487 |
| human cells repair dna damaged by nitrous acid. | cultured fibroblasts from normal persons or persons afflicted by xeroderma pigmentosum were used as hosts for adenovirus 2 infection. with xeroderma cells as hosts, nitrous acid-treated virus showed less plaque-forming ability than when normal cells were used, indicating that dna damaged by nitrous acid is at least partly repaired by normal human cells. | 1975 | 1117879 |
| adenoviruses of chickens: serologic groups. | by literature review and experimental studies, 57 adenovirus isolants from chickens were grouped into 10 distinct serotypes. in plaque-reduction tests, 20 antibody units were reacted with 32-320 plaque-forming units of virus. viruses that were neutralized (80% or greater reduction in titer) by a given serum were considered to belong to that serotype. used to distinguish among serotypes were their antisera. one-way neutralization occurred in only two instances; in both cases it was traced to cont ... | 1975 | 1120041 |
| intermediate in adenovirus type 2 replication. | replicating chromosomes, called intermediate dna, have been extracted from the adenovirus replication complex. compared to mature molecules, intermediate dna had a greater buoyant density in cscl gradients and ethidium bromide-cesium chloride gradients. digestion of intermediate dna with s1 endonuclease, but not with rnase, abolished the difference in densities. these properties suggest that replicating molecules contain extensive regions of parental single strands. although intermediate dna sed ... | 1975 | 1133877 |
| analysis of early adenovirus 2 rna using eco r-r1 viral dna fragments. | adenovirus 2 rna synthesized early in productive infection was analyzed by rna-dna hybridization. hybridization experiments were performed with adenovirus 2 dna and wit, the six adenovirus 2 dna fragments generated 0y digestion with the restriction endonuclease eco r.r1. duplex formation between rna and -32p-labeled viral dna was assayed by s(1) nuclease digestion. rna from the cytoplasm annealed 12 percent of the total viral dna and the following percentage of each of the r.r1 fragments: 6 perc ... | 1975 | 1142475 |
| structural polypeptides of adenovirus-associated virus top component. | | 1975 | 1146228 |
| infectivity of bovine adenovirus type 5 recovered from a polyarthritic calf with weak calf syndrome. | bovine adenovirus type 5, isolated from newborn calves with a polyarthritic disease known as "weak calf syndrome" caused a mild, self-limiting illness in susceptible calves. the induced illness was characterized by marked pyrexia and occasionally by mild diarrhea. it was concluded that the virus may contribute to morbidity and mortality associated with the weak calf syndrome by adding to the stress and debilitation caused by cold wet weather and by bovine viral diarrhea (bvd) virus, which has al ... | 1975 | 1150504 |
| epidemic keratoconjunctivitis: a community outbreak of mixed adenovirus type 8 and type 19 infection. | during the fall and winter of 1973, 145 cases of epidemic keratoconjunctivitis were diagnosed in nashville, tennessee. of the 74 cases studied virologically and/or serologically, 62% were caused by adenovirus type 8, and 28% were caused by adenovirus 19. whereas adenovirus type 8 was isolated only from conjunctival scrapings, adenovirus type 19 was isolated from nose and throat swabs as well as from conjunctivae. the two viruses produced clinically indistinguishable eye disease and household sec ... | 1975 | 1159321 |
| adenovirus binds to rat brain microtubules in vitro. | we have found by negative staining electron microscopy that when similar concentrations of adenovirus and reovirus (viruses of about the same diameter, 75 to 80 nm, and density, 1.34 to 1.36 g/cm3) were incubated with a carbon support film containing microtubules, 72% of adenovirus on the grid, but only 32% (equivalent to random association) of reovirus, were associated with microtubules. similar concentrations of both larger and smaller particles, such as polystyrene latex spheres and coliphage ... | 1975 | 1159898 |
| n6, o2'-dimethyladenosine a novel methylated ribonucleoside next to the 5' terminal of animal cell and virus mrnas. | | 1975 | 1161029 |
| [biological, morphological and immunologic characteristics of cells transformed in vitro by bovine adenovirus type 3]. | studies of cell lines obtained as a result of in vitro transformation by bovine adenovirus type 3 at low multiplicities of infection: bhk-21/13-780 and bhk-21/13-782 (8 x 10(-5) tcd50/cell) and hamster embryo tissue het-1 and het-2 (2 x 10(-3) tcd50/cell) showed them to possess some oncologic characteristics typical of spontaneous neoplasia such as low degree of differentiation, high rate of cell growth, reduced adhesiveness, high tumorigenicity. in hamster embryo tissue cells (het) transformed ... | 1975 | 1162948 |
| methylation of cytoplasmic adenovirus rna synthesized early and late in productive infection. | | 1975 | 1164437 |
| location of sequences on the adenovirus genome coding for the 5.5s rna. | the origin of a low molecular weight virus-associated rna (va-rna) was mapped by hybridization of va-rna to specific fragments of adenovirus type 2 dna, obtained after cleavage with three different restriction endonucleases. va-rna was found to hybridize exculsively to the l-strand [strand with low buoyant density in cscl when complexed with poly(u,g)] of a segment of the viral dna which is located between positions 0.27 and 0.32 on the unit map of the adenovirus type 2 genome. | 1975 | 1164730 |
| adenovirus type 2 dna replication. ii. termini of dna replication. | complete, mature adenovirus type 2 dna molecules were isolated from virus-infected hela cells, pulse-labeled at 20 h postinfection in [3h]thymidine pulses shorter than the time necessary for one round of viral dna replication. after digestion with the restriction endonucleases eco ri, hpa i, and hind iii, a temporal order of synthesis of different regions of the viral genome was established from the relative specific radioactivities in the restriction enzyme fragments. a comparison with the phys ... | 1975 | 1165593 |
| isolation and partial characterization of single-stranded adenoviral dna produced during synthesis of adenovirus type 2 dna. | single-stranded fragments of adenovirus type 2 dna were isolated from infected kb cells under conditions which retarded reassociation of complementary sequences but did not denature native viral dna. of the total intracellular, virus-specific dna labeled during a 1-h pulse with tritiated thymidine begining 15 h after infection, about 20% was single stranded when fractionated on hydroxylapatite. this dna shifted predominantly to the double-stranded fraction on hydroxylapatite during an extended c ... | 1975 | 1165594 |
| adenovirus gene function required for induction of nuclear acidic protein synthesis: binding of these proteins to adenovirus dna. | two different viral dna-defective temperature-sensitive mutants of adenovirus 12 (h 12) were defective in their ability to induce the synthesis of various molecular weight classes of nuclear acidic proteins, both virion and nonvirion components, after lytic infection of human embryo kidney (hek) cells at the restrictive temperature. this finding indicates that the induction of nuclear acidic protein synthesis is an adenovirus gene function(s). treatment of infected cells with actinomycin d at an ... | 1975 | 1165596 |
| identification of self-complementary virus-specific ribonucleic acid in chick kidney cells infected with chicken embryo lethal orphan virus. | rna was extracted from primary chicken embryo kidney (cek) cells infected with chicken embryo lethal orphan (celo) virus and exposed to a pulse of (5-3h)-uridine late in infection. when this rna was self-annealed, 4.5% became resistant to pancreatic ribonuclease digestion. the ribonuclease-resistant rna was isolated by chromatography on sephadex g-100, and the rna was found to have the characteristics of a double-stranded molecule of sedimentation coefficient 8s. half of the column-isolated rna ... | 1975 | 1167576 |
| transcription of the genome of adenovirus type 12. i. viral mrna in abortively infected and transformed cells. | in baby hamster kidney (bkh-21) cells abortively infected with adenovirus type 12, polysome-associated, virus-specific rna could be detected starting 5 to 7 h after infection. the amount of this rna reached a maximum between 10 to 12 h after infection and continued to be synthesized at a reduced level until late in infection (48 to 50 h.). in bhk-21 cells transformed by adenovirus type 12 (hb cells), 0.26% of the polysome-associated mrna was virus specific. the size of the virus-specific mrna is ... | 1975 | 1167602 |
| complementary strands of celo virus dna. | when alkali-denatured dna from celo virus (an avian adenovirus) was annealed for 15 min at 37 c in 0.1 m nacl, 70% of the molecules formed single-stranded circles. this is probably due to base pairing of complementary sequences not more than 110 nucleotides long at the ends of the single strands and implies an inverted terminal repetition in the duplex dna similar to that reported for the dna from human adenoviruses. the circular molecules had a uniform length that was approximately the same as ... | 1975 | 1167606 |
| differential accumulation of virus-specific rna during the cell cycle of adenovirus-transformed rat embyro cells. | in adenovirus type 2-transformed rat embryo cells there is a threefold greater incorporation of [3-h]uridine into virus-specific rna early in s phase than in late s or g2. this heightened accumulation of labeled rna is true for both nuclear and cytoplasmic virus-specific labeling. inhibition of dna synthesis decreases the virus-specific rna labeling, whereas reversal of inhibition again allows the elevated level of virus-specific rna labeling. | 1975 | 1167915 |
| isolation and characterization of bovine adenovirus type 5 associated with "weak calf syndrome". | | 1975 | 1169927 |
| virus specific rna in hamster cells abortively infected with human adenovirus type 12. | | 1975 | 1171554 |
| biochemical studies on bovine adenovirus type 3. i. purification and properties. | bovine adenovirus type 3 (bav3) was purified and its properties were studied. on productive infection of ckt1 cells (a cell line derived from calf kidney) with bav3, it was observed that viral dna synthesis was initiated after about 24 h and its rate was maximal after about 40 h. maturation of the virus occurred several hours after this. purified bav3 was separated into four discrete bands by cscl density gradient centrifugation (complete, incomplete, empty, and degraded viruses). the complete b ... | 1975 | 1171993 |
| biochemical studies on bovine adenovirus type 3. ii. incomplete virus. | incomplete virus of oncogenic bovine adenovirus type 3 (bav3) was highly purified and its biological activity was studied. the production of incomplete virus was found to increase with a high multiplicity of infection and with a large amount of arginine in the growth medium. on infection of contact-inhibited mouse cells, incomplete virus induced cellular dna synthesis and focus formation. moreover, this virus was oncogenic to newborn hamsters. on infection of calf kidney cells, a permissive cell ... | 1975 | 1171994 |
| prevalence of hepatitis b antibody in prostitutes. | the possibility that hepatitis b is transmitted venereally was examined by determining the prevalence of hepatitis b antibody (anti-hb(s)) in populations with different levels of promiscuity. of 258 prostitutes, 31% were anti-hb(s) positive, compared to 10% of 258 female age-matched blood donors, 10% of 48 nuns living in a convent and 15% of 94 nuns working as teachers. prevalence of anti-hb(s) increased with increasing age. in the age group 40-49 years up to 72% of prostitutes were anti-hb(s) p ... | 1975 | 1174290 |
| reye syndrome with associated influenza a and b infection. | in early 1974, seventeen children were treated for reye syndrome. thirteen of these were studied for laboratory evidence of concomitant viral infection. influenza b/hong kong was isolated from the pharynx in four of nine 1974 cases tested. one child had just recovered from varicella. adenovirus type 2 and respiratory syncytial virus were isolated from two additional patients. serologic tests for influenza suggested concomitant or recent influenza b infection in ten of 13 of 1971 cases. during fe ... | 1975 | 1174373 |
| [studies on simian viruses as possible contaminants of inactivated virus vaccines. i. direct and serologic detection of simian adenovirus sv20 (author's transl)]. | in the federal republic of germany inactivated vaccines against poliomyelitis and measles are still produced in monkey kidney cell cultures which may be contaminated by simian viruses. one of these viruses is the oncogenic adenovirus sv20. a control of monkey sera from the institute's monkey house showed a high incidence of hemagglutination-inhibiting and neutralizing antibodies against this virus (table 1). experimentally could be demonstrated that inactivated virus vaccines may be contaminated ... | 1975 | 1179876 |
| [antibody formation and resistance in dogs vaccinated intravenously and subcutaneously against distemper and rubarth's disease]. | the results of the vaccination of dogs with canivac f (distemper vaccine) and canivac fh (distemper) hepatitis vaccine, are presented. the canivac f vaccine was injected intravenously (i.v.) to 8 dogs, intramuscularly (i.m.) to 1 dog and subcutaneously (s.c.) to 1 dog. the canivac fh was administered i.v. to 19 dogs, i.m. to 1 dog and s.c. to 18 dogs. all the dogs displayed no-postvaccination signs. distemper antibodies were observed in blood sera of all dogs vaccinated with canivac f after 9-10 ... | 1975 | 1181557 |
| transcription of the genome of adenovirus type 12. viral mrna in productively infected kb cells. | in human kb cells productively infected with adenovirus type 12, viral dna replication starts between 12 and 14h postinfection. virus-specific, polysome-associated mrna was investigated early (6-8h) and late (26-28h) after infection. most of the viral mrna was polyadenylated and accounted for 0.46% and 24.1% of the mrna synthesized early and late postinfection, respectively. the viral-specific mrna isolated both early and late after infection falls into several distinct size-classes, ranging in ... | 1975 | 1183441 |
| nucleotide sequence homology between a virus-specific 6s rna and various rna species from adenovirus-2-infected kb cells. | to investigate the metabolic fate of a 6s rna (va-rna), which appears in human cell lines only after infection with adenovirus, various species of rna from the cells at different stages of infection were tested for the presence of nucleotide sequence homologous to this rna. rna extracted from subvellular fractions of the cells was further fractionated by sucrose gradient centrifugation and analysed by acrylamide gel electrophoresis or by competition against 32p-labeled va-rna in hybridization wi ... | 1975 | 1183774 |
| demonstration of infectious dna in transformed cells. iii. correlation of detection of infectious dna-protein complexes with persistence of virus in simian adenovirus sa7-induced tumor cells. | primary tumors induced in newborn hamsters by simian adenovirus sa7 were investigated in transfection experiments. infectious dna-protein complexes were readily detected in both the supernatant and pellet fractions obtained by a modified hirt extraction procedure; deae-dextran was required for infectivity to become manifest. infectivity could be abolished by exposure to dnase, but it was unaffected by sa7-specific antiserum or rnase, and only partially inactivated by trypsin treatment. sa7 tumor ... | 1975 | 1184347 |
| [detection of adenovirus type-12 in sarcomas induced by this virus in hamster]. | cells of transplantable sarcomas primarily induced in hamsters with adenovirus, type 12, produced persistantly this virus in an infective form. in sera of sarcomabearing hamsters virus-neutrolizing antibodies and antibodies to t-antigen of tumors were revealed. the presence of t-antigen in cells of all types of sarcoma a-12 was demonstrated by means of immunofluorescence, as well as the absence of a specific luminescence of surface membranes of a-12 mml sarcoma cells, obtained from pulmonary mic ... | 1975 | 1189365 |
| fingerprint analyses of cytoplasmic adenovirus 2 rna size classes synthesized late in productive infection. | | 1975 | 1198928 |
| detection of adeno-associated virus (aav)-specific nucleotide sequences in dna isolated from latently infected detroit 6 cells. | | 1975 | 1198930 |
| [isolation of a virus from sheep and its classification as a new serotype of ovine adenoviruses]. | | 1975 | 1199536 |
| studies of virus-specific antigenicity of tumour cells transformed by bovine adenovirus type 3. | the present paper deals with the study of immunologic relationship between tumours induced by bovine adenovirus type 3 and the organism of the tumour-bearing hamster. the experiments showed that primarily induced tumours were not virus-specifically antigenic as a result of non-expression of weak tsta presumably due to the presence of more potent antigens of cellular origin. it was only after repeated passages of tumour cells in vivo or in vitro, as a result of tumour progression, that the loss o ... | 1975 | 1200779 |
| [frequency of serologic conversion of complement fixing antibodies fc by respiratory viruses in cases of acute juvenile respiratory disease]. | the complement fixation test, run on double samples of serum pertaining to 447 children hospitalized between january 1971 and june 1974 at ospedale maggiore in novara, or checked in consulting rooms for acute phlogosis of the respiratory organs, has permitted to detect in 241 children, equal to the 53,9% of the total cases, a significant increase of antibodies for respiratory viruses (influenza a, b, c, parainfluenza 1, 2, 3. respiratory syncytial virus, adenovirus, reovirus). specifically, in t ... | 1975 | 1205032 |
| an image analysis study of adenovirus type 5-induced crystalline inclusions. | a combined electron microscopic and optical diffractometric study of ad5-induced non-virion protein crystals found in the nuclei of infected kb cells at late times (48 to 72 h p.i.) after infection has been carried out. data obtained have indicated that the unit cell of the crystal is rectangular and not hexagonal as previously presumed. | 1975 | 1206380 |
| viral rna synthesis and levels of dna-dependent rna polymerases during replication of adenovirus 2. | the rates of rna synthesis in cultured human kb cells infected by adenovirus 2 were estimated by measuring the endogenous rna polymerase activities in isolated nuclei. the fungal toxin alpha-amanitin was used to determine the relative and absolute levels of rna polymerases i, ii, and iii in nuclei isolated during the course of infection. whereas the level of endogenous rna polymerase i activity in nuclei from infected cells remained constant relative to the level in nuclei from mock-infected cel ... | 1975 | 1206798 |
| dna-minus temperature-sensitive mutants of adenovirus type 5 help adenovirus-associated virus replication. | efficient potentiation of adenovirus-associated viruses (aav) replication occurs in coinfections with either of two dna-minus temperature-sensitive mutants of adenovirus type 5 (ad5), ts125 and ts149. the helper activity of these mutants does not result from leakiness. at the nonpermissive termperature (39.5 c) there was little or no detectable adenovirus dna synthesis, and only a relatively low level of adenovirus transcription was observed. however, the synthesis of aav dna and rna and the yie ... | 1975 | 1206799 |
| gene functions of adenovirus type 12: synthesis of nuclear acidic proteins in human embryo kidney cells. | | 1975 | 1208875 |
| the development and use of vaccines based on studies of virus substructures. | | 1975 | 1208876 |
| [the inhibiting action of superhigh-frequency millimeter waves on adenovirus (author's transl)]. | virological and biochemical analysis was employed for comparative studies of the content of protein in continuous cultures of human embryo kidney cells (rh line) infected with adenovirus type 1, intact and irradiated with electromagnetic waves of uhf in the range of 5 divided by 8 mm at the density of power current of the order of 4 mw. when the cells were inoculated with intact virus, a cytopatic effect, a decrease in the content of protein by 4 hours postinfection and further decrease up to 72 ... | 1975 | 1210320 |
| [multiplication of adenovirus type 18 in cultures of human fetal trachea]. | | 1975 | 1212342 |
| [electron microscopic study of adenoviral infection in hela cells cultured under conditions of supraoptimal temperature]. | | 1975 | 1214643 |