| production of heat-stable, methanol-soluble enterotoxin by yersinia enterocolitica. | seven isolates of yersinia enterocolitica serotype 0:8, recovered during an outbreak of gastrointestinal illness, were examined for enterotoxin production. all seven strains were enterotoxigenic in the suckling mouse model, and three of five isolates tested produced keratoconjunctivitis in the guinea pig eye model (sereny test). enterotoxin was detected in broth supernatant fluid after 12 h of incubation at 25 degrees c. the toxin was not inactivated by exposure to 121 degrees c for 30 min or by ... | 1979 | 39891 |
| effects of low electrolyte media on salt loss and hemolysis of mammalian red blood cells. | cation loss and hemolysis of various mammalian red cells suspended in isotonic non-electrolyte media were investigated. sucrose buffered with 10 mm tris-hepes, ph 7.4 was used as the non-permeable non-electrolyte. mammals from which the red cells were derived include the human, guinea pig, rat, rabbit, newborn calf, newborn piglet and pig, all of which contain k as the predominant cation species (hk type) and the dog, cat, sheep and cow, all of which possess na as the predominant cation species ... | 1979 | 39943 |
| [stimulating effects of alanine and glycine on guinea-pig taenia coli (author's transl)]. | effects of alanine and glycine on the mechanical and electrical activities of guinea-pig taenia coli were investigated. alanine (0.1--10 mm) and glycine (0.5--10 mm) produced a dose dependent contraction of guinea-pig taenia coli. the stimulating effects of alanine and glycine were not inhibited in the presence of atropine (5 mum), tetrodotoxin (0.1 micrograms/ml), diphenhydramine (4 mum), methysergide (3 mum), strychnine (10 mum), and were not influenced by treatment with indomethacin (3 mum). ... | 1979 | 40057 |
| pharmacological properties of histamine-sensitive adenylate cyclase from guinea pig cardiac ventricular muscle. | | 1979 | 40106 |
| stimulation of beta-adrenoceptors by dobutamine in the guinea-pig atrium and tracheal chain. | | 1979 | 40138 |
| synthesis of enkephalins by guinea-pig striatum in vitro. | a method for obtaining incorporation of labelled amino acids into enkephalins of guinea-pig striatum in vitro and isolating the labelled enkephalins is described. the incorporation of [3h]tyrosine increases with time after a delay of 2 h. cycloheximide added during this time, but not at later stages, inhibits incorporation. the results indicate that the enkephalins are produced locally, probably by ribosomal synthesis. the lag period may be due to the time required for the synthesis of precursor ... | 1979 | 40246 |
| antagonism by some beta-adrenoceptor-blocking agents to cholinergic stimulation of skeletal muscle in vitro. | antiacetylcholine activity some beta-adrenoceptor-blocking drugs was investigated using isolated guinea pig cremaster muscle and frog fectus abdominis muscle. on the cremaster muscle, the antagonism to acetylcholine was non competitive in k0 1313, (+/-)-inpea and (--)-inpea, competitive in (+)-inpea and functional in practolol; all three inpea isomers, practolol and propranolol behaved as noncompetitive antagonists of acetylcholine on frog rectus muscle. caffeine-induced contractions of this mus ... | 1979 | 40268 |
| uptake of putative neurotransmitters in the organ of corti. | in vitro uptake of putative neurotransmitters into the organ of corti of the guinea pig was studied by autoradiography. after incubation in 3h-glycine the label was heaviest over the inner hair cell, but was not confined to the synaptic region of the cell. after incubation in 3h-gaba, 3h-glutamate and 3h-aspartate, heavy labeling was seen over the fibers and terminals of the efferent olivocochlear bundle. leucine, an amino acid not thought to be a neurotransmitter, was uniformly taken up by all ... | 1979 | 40384 |
| effects of adrenergic beta-blockers and a membrane stabilizing agent on ouabain-induced cardiac arrhythmias in anaesthetized guinea pigs. | the antiarrhythmic effects of dl'propranolol, d-propranolol, metoprolol and lidocaine against ouabain-induced cardiac arrhythmias were studied. it was found that contrary to earlier findings in the dog, the effects of the adrenergic beta-blockers against ouabain-arrhythmias in guinea pigs were due to beta-blocking activity and not the membrane-stabilizing activity of the compounds. the cardioselective beta-blocker, metoprolol, was more or equally effective as dl-propranolol against ouabain-induc ... | 1979 | 40389 |
| [effect of vitamin d and vitamin e on the erythrocyte membrane. i. effect on in vitro osmolar resistance and lysis]. | as in the case of retinol, low doses of cholecalcipherol and alpha-tocopherol "in vitro" increase the osmotic resistance of red blood cells, while high doses have a lytic effect on the erythrocytes of various animal species (e.g.: man, sheep, rabbit, guinea-pig, mouse, rat) with the exception of the chicken. the haemolytic effect of cholecalcipherol is less intense than that of retinol; alpha-tochopherol, while causing even less haemolysis than cholecalcipherol, involves a remarkable loss of k+ ... | 1978 | 40412 |
| [effect of antihistaminics on bradykinin action]. | on isolated ileum of the guinea pig the antihistamine drugs dimedrol, diprazin (pipolphen), tavegil and suprastin diminished spasmogenic effects of bradykinin and an enhancement of microvascular permeability induced by this polypeptid. the antihistamine drugs display a nonspecific antibradykinin activity. no relationship between the chemical structure and pronounced antibradykinin action of the antihistamines studied was revealed. | 1979 | 40631 |
| comparative studies on immunologically and non-immunologically produced slow-reacting substances from man, guinea-pig and rat. | 1 slow-reacting substance of anaphylaxis (srs-a) was produced by antigen challenge of passively sensitized human lung and actively sensitized guinea-pig lung. 2 a slow-reacting substance (srs) was prepared from the peritoneal fluid of rats treated with calcium ionophore a23187. 3 these substances were extensively purified by charcoal adsorption, sephadex g-15 gel filtration, ether extraction and reverse phase high pressure liquid chromatography. 4 the three substances are pharmacologically, chem ... | 1979 | 40646 |
| the effects of high pressure helium and nitrogen on the release of acetylcholine from the guinea-pig ileum. | 1 the effects of high pressures of helium and of nitrogen on acetylcholine release were tested using the guinea-pig ileum as a model preparation. a superfusion system was designed in which this tissue could be maintained under physiological conditions in a high pressure chamber.2 helium, at a pressure of 136 atm slightly increased the spontaneous output of acetylcholine but produced no significant changes at 68 atm (136 atm is close to the lethal pressure for small mammals).3 the acetylcholine r ... | 1979 | 40648 |
| strain differences in adrenal xenobiotic metabolism in guinea pigs. | studies were carried out to compare adrenal and hepatic xenobiotic metabolism in various strains of guinea pigs. in all strains studied (hartley, english short hair, nih, strain 2, strain 13), microsomal protein and cytochrome p-450 levels and nadph-cytochrome c reductase activities were greater in adrenals than livers. neither adrenal values nor hepatic values for these parameters differed across strains. ethylmorphine (em) demethylase and benzo[a]pyrene (bp) hydroxylase activities were also gr ... | 1979 | 40764 |
| [antihistamine and antiserotonin action of 2 aminotetralin derivatives]. | the authors performs studies under the conditions of whole organism in vivo and on isolatid organs in vitro and examined antihistamine and antiserotonine activity of two derivatives of 2-aminotetraline, synthesized by d. dancev and iv. ivanov, namely: n-(trans-e-hydroxy-1, 2, 3, 4-tetrahydro-2-naphtyl (-n-) 3-oxo-3-phenylpropyl) piperasine dichloride and n-(trans-3-hydroxy-1, 2, 3, 4-tetrahydro-2-naphtyl (-n-) 3-oxo-3-3phenyl-2-methylpropyl (piperasine dichloride) indicated respectively as p10 a ... | 1979 | 40787 |
| guinea pig liver aromatic aldehyde-ketone reductases identical with 17 beta-hydroxysteroid dehydrogenase isozymes. | two nadph-dependent aromatic aldehyde-ketone reductases purified from guinea pig liver catalyzed oxidoreduction of 17 beta-hydroxysteroids and 17-ketosteroids. one enzyme efficiently oxidized 5 beta-androstanes and reduced 17-ketosteroids of a/b cis configuration, whereas the other enzyme efficiently oxidized 5 alpha-androstanes and equally reduced both 5 alpha-and 5 beta-androstanes of 17-ketosteroids. however, aromatic aldehydes and ketones, and 3-ketosteroids were irreversibly reduced by the ... | 1979 | 40969 |
| pa2 values of selective beta-adrenoceptor antagonists on isolated atria demonstrate a species difference in the beta-adrenoceptor populations mediating chronotropic responses in cat and guinea-pig. | pa2 values for atenolol (beta 1-selective) and alpha-methylpropranolol (beta 2-selective) have been determined on isolated atria of cat and guinea-pig using noradrenaline (beta 1-selective) and fenoterol (beta 2-selective) as agonists. on guinea-pig atria, the pa2 values did not vary with the agonist used. on cat atria the pa2 for atenolol was greater with noradrenaline than with fenoterol and the pa2 for alpha-methylpropranolol was greater with fenoterol than with noradrenaline. fenoterol was 2 ... | 1979 | 41041 |
| responses of guinea-pig lung parenchymal strips to prostaglandins and some selected autacoids. | | 1979 | 41051 |
| cardiovascular studies with impromidine (sk&f 92676), a new very potent and specific histamine h2-receptor agonist. | impromidine (sk & f 92676) has recently been identified as a potent and specific histamine h2-receptor agonist. the present paper describes some cardiovascular studies with the drug. impromidine lowers blood pressure in cats and rats by interaction with h2-receptors. during continuous intravenous infusions of impromidine, the fall in blood pressure is due to a reduction in total peripheral resistance; cardiac output increases during hypotension. the responses to impromidine are similar to respon ... | 1979 | 41056 |
| non-competitive-non-equilibrium alpha-adrenoceptor blocking properties of n-benzyl iodoacetamide, betsamide. | n-benzyl iodoacetamide, betsamide, at 10 mg kg-1 i.v. blocked the hypertensive and contractile responses of the nictitating membrane of the cat to adrenaline. the blockade had a lag period before full development. pretreatment of cats with betsamide for 7 or 18 h showed a non-equilibrium type of alpha-adrenoceptor blockade. the responses of the nictitating membrane to adrenaline were markedly depressed and did not recover after high doses of adrenaline. in the same cats, adrenaline caused a prof ... | 1979 | 41059 |
| the nature of the negative endocochlear potentials produced by anoxia and ethacrynic acid in the rat and guinea-pig. | 1. the alterations in the na+ and k+ concentrations of the cochlear endolymph and in the endocochlear potential were followed simultaneously by means of ion-sensitive and conventional micro-electrodes during simple anoxia, during anoxia after i.v. ethacrynic acid and after i.v. ethacrynic acid alone. the endolymphatic ph changes were measured separately and the effect of perilymphatic ethacrynic acid upon the endocochlear potential was investigated. 2. the over-all na+:k+ permeability ratio for ... | 1979 | 41092 |
| electromechanical effects of ph in guinea-pig cardiac ventricular muscle [proceedings]. | | 1979 | 41096 |
| the importance of choice of agonist in studies designed to predict beta 2 : beta 1 adrenoceptor selectivity of antagonists from pa2 values on guinea-pig trachea and atria. | 1. pa2 values have been obtained for propranolol, butoxamine, h35/25 and atenolol on guinea-pig isolated trachea and atria (rate) using noredrenaline (beta 1-selective), isoprenaline (non-selective) and fenoterol (beta 2-selective) as agonists. 2. pa2 values varied with the agonist used on trachea but not on atria and, therefore, trachea : atria selectivity values varied with the agonist used. 3. it is suggested that the best estimate of the selectivity of an antagonist between beta 2- and beta ... | 1979 | 41190 |
| leukotriene c: a slow-reacting substance from murine mastocytoma cells. | murine mastocytoma cells treated with calcium ionophore a23187 produced a slow-reacting substance (srs) that caused guinea pig ileum to contract. the response was reversed by the srs antagonist fpl 55712. on the basis of isotope incorporation experiments, spectroscopy, and chemical degradations, the srs was identified as a cysteine-containing derivative of 5-hydroxy-7,9,11,14-icosatetraenoic acid. this amino acid was attached in thioether linkage at c-6. the srs is structurally related to previo ... | 1979 | 41240 |
| specific protection of the binding sites of d-ala2-d-leu5-enkephalin (delta-receptors) and dihydromorphine (mu-receptors). | phenoxybenzamine causes a long-lasting inactivation of the opiate receptors of the mu- and delta-type in homogenates of guinea-pig brain. the effect is selectively prevented when, before exposure to phenoxybenzamine, the homogenate is pre-incubated with ligands of high affinity for either of the two binding sites, i.e. dihydromorphine for the mu-receptor and tyr-d-ala-gly-phe-d-leu for the delta-receptor. in contrast, tyr-d-ala-gly-phe-l-leu amide, which has high affinities for both binding site ... | 1979 | 41257 |
| co2-induced kidney calcification. | light microscopic examination of kidney tissue of guinea pigs exposed to 1.5% co2, 21% o2, and balance n2 for periods as long as 42 days and of rats exposed to the same co2 concentrations for up to 91 days showed that the incidence of focal kidney calcification increased with length of exposure. calcification occurred primarily in the tubules of the renal cortex. another group of guinea pigs were exposed to 1% co2, 21% o2, and the balance n2 for periods up to six weeks and were later killed at r ... | 1979 | 41351 |
| effects of clonidine, guanfacine and three imidazolidine derivatives related to clonidine on blood pressure, heart rate and gastric acid secretion in the anaesthetized rat. | the effects of histamine, guanfacine, clonidine (2,6-dichlorophenylimino-2-imidazolidine) and the 2,6-dibromo, 2,3- and 2,5-dichloroanalogues of clonidine were assessed on blood pressure, heart rate and gastric acid secretion in the anaesthetized rat, with lumen-perfused stomach. histamine (100 microgram/kg to 1 mg/kg) and clonidine (250 microgram/kg to 1 mg/kg) each caused acute increases in acid secretion, the magnitude and duration of which were dose-dependent: the secretory effect of clonidi ... | 1979 | 41494 |
| intracellular localization of long-chain acyl-coenzyme a hydrolase and acyl-l-carnitine hydrolase in brown adipose tissue from guinea pigs. | the activities of long-chain acyl-coa hydrolase (palmitoyl-coa hydrolase, ec 3.1.2.2) and long-chain acyl-l-carnitine hydrolase, ec 3.1.1.28) in brown adipose tissue from cold-exposed and control guinea pigs were studied. mitochondria from cold-exposed animals hydrolysed 21 nmol of palmitoyl-coa/min per mg of protein and 1.3 nmol of palmitoyl-l-carnitine/min per mg of protein, and the specific activities were respectively 2 and 5 times as high in cold-exposed as in control animals. the subcellul ... | 1979 | 41517 |
| tricyclic antidepressants: potent blockade of histamine h1 receptors of guinea pig ileum. | six tricyclic antidepressants were tested for their ability to antagonize histamine actions at histamine h1 receptors in a bioassay for these receptors (histamine-induced contractions of guinea pig ileum). all compounds were competitive antagonists with equilibrium dissociation constants in the range of 5.6 x 10(-11) m to 1.5 x 10(-7) m. doxepin hydrochloride and amitriptyline hydrochloride were the most potent compounds of the series and may be the most potent antihistamines known. antagonism a ... | 1979 | 41726 |
| postcapillary ph disequilibrium after gas exchange in isolated perfused liver. | ph equilibration after gas exchange in a systemic vascular bed was investigated using an isolated guinea pig liver preparation perfused with a blood-free krebs-ringer-bicarbonate solution. effluent perfusate was withdrawn into a stopped-flow apparatus in which ph and temperature were continuously monitored. a decrease in ph of the perfusate after exit from the liver was observed. addition of acetazolamide to inflowing perfusate had little effect on this fall in effluent fluid ph, while the addit ... | 1979 | 41831 |
| purification and some physicochemical properties of slow-reacting substance of anaphylaxis (srs-a) from sensitized guinea pig lung. | | 1979 | 42007 |
| action of histamine on cyclic amp in guinea pig gastric cells: inhibition by h1- and h2-receptor antagonists. | | 1979 | 42011 |
| the schultz-dale reaction of the guinea-pig ileum: influence by beta-adrenoceptor agonists and theophylline. | one of the organs that can be used to study the schultz-dale reaction, is the guinea-pig ileum. the reaction is characterized by a specific pattern: a quick contraction, followed by a quick relaxation and a second, more slow contraction. a description is given of this typical reaction. selective inhibition of the slow contraction could be obtained using beta-adrenoceptor agonists. also theophylline inhibited the slow contraction, but this inhibition was only selective within a narrow range of co ... | 1979 | 42288 |
| production, purification and characterization of rat interferon. | priming with heterologous mouse interferon, increased production of an antiviral substance induced in rat diploid fibroblasts by newcastle disease virus. this substance was characterized as an acid stable interferon. this rat interferon exhibited marked cross-species antiviral activity when tested in mouse l929b cells, guinea pig embryo fibroblasts, human fibroblasts and bovine cells but was not active on chick embryo cells. | 1979 | 42377 |
| stimulation of phosphatidylinositol turnover in various tissues by cholinergic and adrenergic agonists, by histamine and by caerulein. | studies are reported of the biochemical and pharmacological characteristics of the stimulation of phosphatidylinositol metabolism that is produced in appropriate target tissues by stimulation of various receptors that use ca(2+) as their second messenger. (1) muscarinic cholinergic and alpha-adrenergic phosphatidylinositol responses were observed in rat lacrimal gland, and a response to caerulein was detected in the longitudinal smooth muscle of guinea-pig ileum. (2) the muscarinic cholinergic p ... | 1979 | 42389 |
| the in vivo examination of the irreversible beta-adrenoceptor antagonist ro 03-7894 on cardiac rate and contractility. | 1. two benzofuran-2-ethanolamines ro 03-5255 (1-(5-acetylamino-benzofuran-2-yl)-1-hydroxy-2-isopropylaminoethane) and ro 03-7894 (1-(5-chloracetyl aminobenzofuran-2-yl)-1-hydroxy-2-isopropylaminoethane) which had previously been shown to exhibit respectively competitive and irreversible beta-adrenoceptor antagonism in guinea-pig isolated atria, were compared in vivo using isoprenaline-induced tachycardia of anaesthetized guinea-pigs and heart rate and contractility (dp/dtmax) of open-chest anaes ... | 1979 | 42499 |
| phenotypically determined resistance of neisseria gonorrhoeae to normal human serum: environmental factors in subcutaneous chambers in guinea pigs. | some gonococci obtained from human urethral exudate or from subcutaneously implanted chambers in guinea pigs show a resistance to killing by human serum which is lost on sub-culture in vitro after a few generations. the environmental factors which may influence the phenotypic expression of resistance to serum killing were investigated in guinea pig chambers and in chamber fluid in vitro. the redox potential in chambers before and after infection was lower than that of heart blood but conditions ... | 1979 | 42666 |
| effects of the antiemetic drug domperidone on the rate of gastric emptying in the guinea-pig [proceedings]. | | 1979 | 42686 |
| histamine receptors in the guinea pig ileum. | histamine and some related compounds acting selectively on h2-or h1 receptors were tested for their ability to contract the guinea pig ileum, in the usual whole ileum preparation and in the longitudinal muscle preparation. the concentrations elicited by histamine in both kinds of preparations were not potentiated by cimetidine or metiamide and were not inhibited by administration of h2 receptor selective agonists in doses which were subthreshold for contracting the guinea pig ileum; higher doses ... | 1979 | 42850 |
| digoxin induced release of creatine kinase from isolated guinea-pig hearts. | in isolated perfused guinea-pig hearts, digoxin produced a concentration dependent release of creatine kinase (atp-creatine-transphosphorylase; ck). a corresponding decrease of the ck activity in the myocardium was obtained. the enzyme release seems to be a sign of glycoside intoxication, as its extent paralleled the severity of digoxin induced arrhythmias. especially high ck activities were liberated when ventricular fibrillation occurred. likewise, electrically induced fibrillation, in control ... | 1979 | 42852 |
| quantitative relationship between beta-adrenergic receptor number and physiologic responses as studied with a long-lasting beta-adrenergic antagonist. | the aminobenzyl analog of propranolol, 1- (p-amino-alpha,alpha-dimethylphenethylamino)-3-(1-naphthoxy)-2- propanol, was synthesized and found to be a potent beta-adrenergic blocking agent. the beta-adrenergic receptors of cultured rat c6 glioma cells (2b clone) as assessed by [(125)i]iodohydroxybenzylpindolol binding were decreased 50 and >95% after pretreatment with 8 nm and 1 mum aminobenzylpropranolol, respectively. unlike propranolol, aminobenzylpropranolol displayed a prolonged blockade of ... | 1979 | 42915 |
| the action of substance p on neurons of the myenteric plexus of the guinea-pig small intestine. | extracellular and intracellular recordings were made in vitro from single neurons of the myenteric plexus of the guinea-pig small intestine. synthetic substance p was applied to the neurons by means of the perfusing solution or by electrophoresis from micropipettes. extracellular recording showed that substance p (100 pm-30 nm), applied by perfusion, increased the firing rate of myenteric neurons. intracellular recording indicated that perfusion with substance p caused a dose-dependent membrane ... | 1979 | 42923 |
| dual histamine receptor mechanism in guinea-pig lung. | isolated guinea-pig lung parenchymal strips (gpls) relaxed in response to the selective histamine h2-receptor agonists, dimaprit and 4-methylhistamine (4-meh), and to low doses of histamine (10(-9) to 10(-7) mol/l) and contracted in response to several spasmogens. the order of the relative activity of the spasmogens was 2-methylhistamine (2-meh) greater than histamine greater than carbachol greater than 2-pyridylethylamine (2-pe). dimaprit and 4-meh also relaxed gpls which were contracted by 2-m ... | 1979 | 42932 |
| the shape, polarization, and innervation of sensory hair cells in the guinea pig crista ampullaris and macula utriculi. | | 1979 | 42972 |
| on the range of alpha-adrenergic regulation of coronary vascular resistance. | in order to obtain an estimate of the range of alpha-adrenergic resistance regulation in the coronary vascular system, the following studies were performed: in 15 anesthetized dogs the circumflex coronary artery was cannulated and perfused with above-normal constant pressure to that coronary venous oxygen tension never fell below 40 mmhg. thus, it was possible to eliminate the influence of the metabolic factor regulating coronary resistance. furthermore, 15 isolated isovolumetrically working gui ... | 1979 | 43128 |
| the actions of 5-hydroxytryptamine and histamine on the isolated ileum of the tree shrew (tupaia glis). | 1. contractions to 5-hydroxytryptamine (5-ht) and histamine of longitudinal muscle from the isolated ileum of the tree shrew (tupaia), guinea-pig and rat were investigated by constructing dose-response curves and studying the effects of various antagonists. 2 in the tupaia and rat ileum the contraction to 5-ht was reduced by methysergide but not affected by tetrodotoxin (ttx), morphine, hexamethonium (c6) or atropine. the response of guinea-pig ileum to 5-ht was not significantly inhibited by me ... | 1979 | 43173 |
| metabolism of phenothiazine in the guinea pig. | the metabolism of phenothiazine was studied in the guinea pig. in the adult animal after 24 hr, about 7% of the administered dose was found in feces, with traces of the sulfoxide and phenothiazone. small amounts of all these compounds also were found in urine, with leucophenothiazone sulfate (approximately 12%) and a metabolite identified as phenothiazine n-glucuronide (approximately 22%). this latter compound was also present in bile. both phenothiazine and its sulfoxide were retained in body t ... | 1979 | 43227 |
| methysergide induces selective potentiation in cholinergic contractions of the guinea-pig vas deferens by facilitating acetylcholine release. | methysergide (3 x 10(-6) m) enhanced the contractile responses of the isolated stripped vas deferens of guinea-pig to acetylchline(ach) and arecoline, but not those to noradrenaline, tyramine and bradykinin. methysergide (3 x 10(-5) m) suppressed the contraction elicited by noradrenaline or histamine. the methysergide-induced potentiation of the response to ach was prevented by pre-addition of hemicholinium but not by tetrodotoxin or morphine. the augmentation of the response to ach by physostig ... | 1979 | 43366 |
| haemolysis of various mammalian erythrocytes in sodium chloride, glucose and phosphate-buffer solutions. | mouse, rat, rabbit, hamster, cow, pig, sheep, guinea-pig, dog and human erythrocytes were studied. a 0.9% or stronger solution of sodium chloride completely prevented haemolysis; sheep and pig erythrocytes appeared the more fragile, while human and dog erythrocytes were not haemolized in concentrations of 0.4% or more. haemolysis of human, rabbit, cow, hamster, guineapig, pig and sheep erythrocytes was not observed in solutions of 0.4% or more of glucose. except for sheep, human and dog erythroc ... | 1979 | 43414 |
| binding of 3h-clonidine to an alpha-adrenoceptor in membranes of guinea-pig ileum. | | 1979 | 43474 |
| increase by alpha-adrenolytic drugs of acetylcholine release evoked by field stimulation of the guinea-pig ileum. | the release of acetylcholine evoked by field stimulation of the guinea-pig ileum (3 hz) is increased by yohimbine and tolazoline but not affected by phentolamine. it is proposed that yohimbine and tolazoline by blocking alpha-adrenoceptors of the cholinergic nerves abolish the inhibition caused by endogenous noradrenaline, and thus facilitate the output of acetylcholine. | 1979 | 43477 |
| arachidonic acid activation of guinea pig lung guanylate cyclase by two independent mechanisms. | the purpose of this study was to elucidate the mechanisms by which arachidonic acid activates guanylate cyclase from guinea pig lung. guanylate cyclase activities in both homogenate and soluble fractions of lung were examined. guanylate cyclase activity was determined by measuring formtion of [32-p] cyclic gmp from alpha-[32-p] gtp in the presence of mn2+, a phosphodiesterase inhibitor and a suitable gtp regenerating system. arachidonic acid, and to a slight extent dihomo-gamma-linolenic acid, a ... | 1979 | 43557 |
| [the behavior of sensory and ganglia cells of the vestibular apparatus of the guinea pig in response to inadequate stimulations]. | the behaviour of the sensory- and the ganglia cells in response to inadequate stimuli was studied at 12 adult guinea pigs. the sensory cells of the three cristae ampullares dont respond remarkably to acoustic stimulation. at the range of 1000 hz a recognizable increase in the size of the sensory cells at the upper ampulle was noticed. of no account was the rest of the volume variations. at the range of 5000 hz the sensory cells of the maculae staticae responded with a considerable increase in th ... | 1979 | 43629 |
| alterations of two parameters of cellular immunity in the aged men. | there were two parameters of cellular immunity in 46 old and 34 adult (control) individuals investigated. these reactions were delayed type hypersensitivity of the skin and the in vitro leukocyte migration inhibition test. ppd was used as antigen for both reactions. according to our investigations there was no correlation between them neither in the aged nor in the control group. but the statistical analysis of data revealed a significant difference between skin responses of aged and control gro ... | 1979 | 43677 |
| the function of fimbriae in myxococcus xanthus. ii. the role of fimbriae in cell-cell interactions. | anti-fimbriae antiserum specifically inhibited swarming but no gliding motility per se in myxococcus xanthus. however, formation of motile aggregates on agar and clumps in liquid media correlated with the presence of fimbriae. ethylenediaminetetraacetic acid which inhibited swarming also inhibited fimbriae formation. direct electron-microscopic observations revealed that fimbriae establish contact with apposing cell surfaces. intact but not depolymerized fimbriae exhibited hemagglutination activ ... | 1979 | 43768 |
| inhibitory alpha-adrenergic action of phenylephrine in guinea pig taenia caecum. | phenylephrine, a selective alpha-adrenergic stimulant, caused a maximal relaxation of the taenia from guinea pig caecum in the concentration of 10(-6) g/ml. phenylephrine in this concentration did not influence intracellular cyclic amp and cyclic gmp levels. although phenylephrine abolished the spontaneous spike discharge, no change was detected in 45ca-uptake and 45ca-efflux on the tissue level after phenylephrine. ca-uptake and ca-release on the subcellular level were also not influenced by ph ... | 1979 | 43911 |
| effects of palytoxin on mechanical and electrical activities of guinea pig papillary muscle. | effects of palytoxin (ptx) on isolated papillary muscles of guinea pigs were studied in an attempt to elucidate the mechanical and electrical activities. inotropic effects of ptx above 3 x 10(-9) g/ml; an early positive inotropic effect, slowly developing contracture accompanied by decline in phasic tension, appearance of aftercontractions and arrhythmias at high doses. the positive inotropic effect of ptx was enhanced in high ca2+ medium but was not modified by propranolol. ptx induced a sustai ... | 1979 | 43916 |
| ixodes ricinus: vector of a hitherto undescribed spotted fever group agent in switzerland. | a tick/rickettsial survey in various parts of switzerland revealed the presence of a new, hitherto undescribed spotted fever group rickettsia ("swiss agent") in up to 11.7% of i. ricinus collected off vegetation. infection in ticks was found to be generalized with rickettsiae developing intracellularly and occasionally also intranuclearly. as a result of massive growth in ovarial tissues, including the germinative cells, the rate of transovarial and filial infection was 100%. the "swiss agent" a ... | 1979 | 44100 |
| [a review of pharmacological studies on nicergoline]. | 10-methoxy-1,6-dimethyl-ergoline-8 beta-methanol-(5-bromonicotinate) (nicergoline, sermion) shows a strong alpha-blocking activity both in vitro and in vivo. various studies (dog, cat, rabbit, rat, mouse, guinea-pig) show that nicergoline affects only slightly blood pressure and heart rate and increases the blood flow in brain and hind limb without affecting the splanchnic and aortic flow in normal animals. nicergoline does not interfere with cns functions unless applied in high doses. it stimul ... | 1979 | 44197 |
| antiarrhythmic activity of dextro- and levo-isomers of 5-methyl-8-(2-hydroxy-3-t-butylamino-propoxy) coumarin hydrocholoride (bucumolol), a beta-adrenergic blocking agent, on aconitine-induced atrial and ouabain-induced ventricular arrhythmias in dogs. | the beta-blocking, antiarrhythmic, and local anesthetic effects of the racemic mixture and optical isomers of bucumolol, 5-methyl-8-(2-hydroxy-3-t-butylamino-propoxy) coumarin hydrochloride, were studied in dogs, guinea-pigs and frogs. in blocking the positive chrontropic response to isoproterenol, the levo-isomer of bucumolol was about 40 times more potent in dogs, and 270 times in guinea-pigs than its dextro-isomer and twice as effective in both species as the racemic mixture. in frog sciatic ... | 1979 | 44326 |
| characterization of the two major species of slow reacting substance from rat basophilic leukemia cells as glutathionyl thioethers of eicosatetraenoic acids oxygenated at the 5 position. evidence that peroxy groups are present and important for spasmogenic activity. | the most prominent slow reacting substance from rat basophilic leukemia cells (type i) was characterized by radiochemical, chemical and physical methods and shown to contain a c20 unsaturated fatty acid oxygenated at the 5 position and a sulfur containing side chain in thioether linkage at the 6 position. its spasmogenic action on guinea pig ileal muscle was largely inactivated under reducing conditions which suggested that a peroxy group was present and important for contractile activity. this ... | 1979 | 44377 |
| [oxidative phoshporylation, proton exchange and volume changes in hepatocyte mitochondria under the effect of exogenous succinate]. | long use of exogenous succinate causes changes in hepatocytes bioenergetic functions in guinea pigs. the character of cation is of essential significance in this situation: sodium succinate produces a moderate stimulating effect, potassium succinate evokes a distinct energizing influence. | 1979 | 44392 |
| enzyme histochemistry of induced heterotropic bone formation in guinea-pigs. | | 1979 | 44466 |
| effect of histamine & albizzia lebbek benth. on guineapig adrenal glands. | | 1979 | 44515 |
| effect of histamine and stress on the gastric mucosal ca2+ content during the development of gastric ulcers. | the influence of histamine, its triazole derivative (3-beta-aminoethyl-1,2,4-triazole) and immobilization stress on the gastric mucosal ca2+ content during the development of gastric ulcers in guinea pigs and rats was investigated. a considerable fall in the concentration of ca2+ in gastric tissues of guinea pigs after administration of histamine 0.25 mg/kg, down to 80% (8.0 mumol/g), its derivative (1 mg/kg) to 72% (7.2 mucol/g) and stress to 76% (7.6 mumol/g) was recorded by atomic absorption- ... | 1979 | 44658 |
| mammalian serum susceptibility of serratia marcescens: detection of three human serum susceptibility categories. | a total of 65 strains of s. marcescens, including 36 currently employed o- and h-antigen reference strains, were examined for their susceptibility to the bactericidal activity of 80 vol % of fresh human serum. the majority of strains (57 = 87.6%) proved 'delayed serum-sensitive' (dss); 4 strains (6.2%) were 'promptly serum-sensitive' (pss), whereas 4 strains (6.2%) resisted (nss) complement-mediated killing by human serum. fresh sera obtained from 7 adult human volunteers yielded essentially ide ... | 1979 | 44784 |
| isolation of antigenic variants from leptospiras grown in vitro and from heart blood of guinea pigs inoculated with a clonized strain of leptospira. | a clonized culture of leptospira interrogans serovar copenhageni strain shibaura (cl-shibaura) was inoculated into guinea pigs. the heart blood of the guinea pigs, obtained at the febrile stage and inoculated onto the solid serum medium containing the homologous immune serum, produced large and small colonies. the serological examinations revealed that the large colonies were found mainly to be the antigenic variants, while the small colonies were mostly the parent. the antigenic variants accoun ... | 1979 | 44789 |
| synthesis and degradation of nad in guinea pig cardiac muscle: ii. studies about the different biosynthetic pathways and the corresponding intermediates. | | 1979 | 44842 |
| pharmacological reactions of the circular muscle of the guinea pig vas deferens. | pharmacological responses of spiral strips prepared from the guinea pig vas deferens to various adrenergic and cholinergic agonists and autacoids were studied. on the circular muscle alpha adrenergic, muscarinic cholinergic and histaminergic receptors were identified. the responses evoked on the circular and longitudinal muscles were of the same type. | 1979 | 44960 |
| bicarbonate atp-ase in ciliary body and a theory of diamox effect on aqueous humor formation. | bicarbonate was found to stimulate atp breakdown by rabbit or cat ciliary body-iris homogenates. maximum hco3- stimulation of atpase with tris-hepes buffer occured at ph 8.0. acid ph and chloride ions in the media reduced the activity of the hco3--stimulated atpase. the km for atp was 0.55 mmolar and for hco3-, 20 mmlar. hco3- atpase was not inhibited by acetazolamide added to in vitro. it is postulated that atpase represents the linkage step of energy donor mechanism and active ct secretion in ... | 1979 | 45223 |
| [sympathetic mediation of induced and spontaneous activity of the pelvis-ureter in vitro]. | the spontaneous activity of guinea-pig renal pelvis-ureter is regulated by the adrenergic system. spontaneous rhythmic contractions, and contractions induced by noradrenaline are inhibited by dihidroergotamine and phentolamine. alpha-adrenergic blocking agents block also contractions induced by histamine, angiotensin and barium chloride, but not contractions induced by electric stimulation. the authors suggest an hypothetical model for the activation of the adrenergic receptor: noradrenaline (ne ... | 1979 | 45305 |
| zoological specificity of human thyroid-stimulating antibody. | the zoological specificity of human thyroid-stimulating antibody (tsab) that occurs in the blood in graves' disease was examined by assessing its effect on the thyroid of the dog, guinea pig, calf, and mouse as well as that of man. with all but murine glands, thyroid stimulation was assayed by measuring the increase in the concentration of camp in the thyroid slices or fragments after 2 h of incubation in buffer containing tsab. effects on the thyroid of the mouse were monitored by the in vivo b ... | 1978 | 45462 |
| composition of glycoproteins secreted by tracheal explants from various animal species. | 1. the acidic and neutral glycoproteins secreted by cultured tracheal explants from pigs, sheep, rats, mice, monkeys, guinea pigs, dogs and chickens were purified and fractionated by column chromatography on deae-cellulose and by electrophoresis on cellulose acetate. 2. the ratios of acidic to neutral mucus glycoproteins were compared for the above animals with that of mucus glycoproteins secreted by cultured human bronchi. 3. the observed ratios of acidic to neutral glycoproteins ranged from 4. ... | 1979 | 45551 |
| [histologic-histochemical findings in the salivary glands of guinea pigs after sectioning the chorda tympani]. | we investigated gl. submandibularis and gl. sublingualis of the guinea-pig 1, 2, 5, 7, 14 und 28 days after section of the chorda tympani with histological-histochemical methods. the innervation pattern of both glands (gl. submandibularis: aminergic-cholinergic double innervated; gl. sublingualis: cholinergic innervated) remains unchanged. in the gland cells the following effects were observed: a) gl. sublingualis. in the first 3 days apocrine and holocrine secretion phenomena are often seen, su ... | 1977 | 45627 |
| enhancement of macrophage adenylate cyclase by microtubule disrupting drugs. | the accumulation of cyclic adenosine 3',5'-monophosphate (camp) in guinea-pig macrophages exposed to the adenylate cyclase (ac) stimulators prostaglandin e1 (pge1) and isoproterenol (ip), was markedly enhanced by pretreatment of the cells with colchicine, vinblastine, and podophyllotoxin--agents which prevent microtubule assembly. the same agents did not augment basal camp levels. the facilitating effect of the drugs on the response to pge1 and ip developed both in the absence and presence of a ... | 1978 | 45791 |
| the variable fine structure of elastin visualized with verhoeff's iron hematoxylin. | verhoeff's iron hematoxylin (vih) followed by lead citrate (lc) applied to epoxy thin sections stained the dense component of elastic fibers heavily and the peripheral microfibrillar component lightly in guinea pig trachea and mouse testis fixed with a glutaraldehyde-osmium tetroxide sequence. this method stained large fimbriated fibers beneath tracheal epithelium, small fibers and stacked aggregates thereof in the deep lamina propria, cartilage and adventitia of the trachea and large stacked fi ... | 1975 | 45879 |
| antagonism of slow reacting substance in anaphylaxis (srs-a) and other spasmogens on the guinea pig tracheal chain by hydratropic acids and their effects on anaphylaxis. | | 1975 | 45882 |
| glycocalyx of epidermal cells in vitro: demonstration and enzymatic removal. | guinea-pig epidermal cells in culture possess a glycocalyx coat similar to that in vivo, as revealed by the ruthenium red stating technique. trypsin, phospholipase c, and lysozyme do not produce any changes of the glycocalyx, while hyaluronidase and neuraminidase lead to partial and subcomplete removal respectively. cells stripped of their glycocalyx coat by neuraminidase do not detach from the support and do not show any signs of toxicity. there is complete reconstitution of the glycocalyx with ... | 1975 | 45927 |
| comparative studies of guinea pig and bovine myelin basic proteins. partial characterization of chemically derived fragments and their encephalitogenic activities in lewis rats. | | 1975 | 45932 |
| effect of ochratoxin and aflatoxin on serum proteins, complement activity, and antibody production to brucella abortus in guinea pigs. | the effect of ochratoxin alone and in combination with aflatoxin and brucella abortus antigen on complement activity, serum proteins, and antibody response in guinea pigs was investigated. ochratoxin did not affect complement activity or antibody response and there was no interaction between ochratoxin and aflatoxin on any of the responses tested. ochratoxin significantly lowered the level of beta-globulin in serum of guinea pigs. there was no significant interaction between aflatoxin and antige ... | 1975 | 45955 |
| purification, characterization and radioimmunoassay of thyrocalcitonin from rat thyroid glands. | a highly purified preparation of rat thyrocalcitonin (tct) has been obtained from lyophilized thyroid glands by gel chromatography following acid-acetone extraction. biological activity of sephadex g-50 eluates appeared in two peaks. the tct in the major peak was concentrated, and applied to a bio-gel p-6 column, and a major protein peak was eluted which coincided with tct activity. potency, estimated by bioassay in rats, increase approximately 3500-fold from 0.075 mrc u/mg lyophilized glands to ... | 1975 | 46198 |
| interaction of complex polysaccharides with the complement system: effect of calcium depletion on terminal component consumption. | complex polysaccharides and lipopolysaccharides can activate the terminal components of complement by either the classical (antibody, c1, c4, and c2) or alternative complement pathways, but the relative importance of either pathway for terminal component consumption in normal serum is poorly understood. since classical complement pathway function requires both calcium and magnesium ions, whereas the alternative pathway requires only magnesium ions, selective chelation of calcium ions in serum ca ... | 1975 | 46215 |
| allergic encephalomyelitis. isolation of an encephalitogenic peptide active in the monkey. | a 17-residue peptide (peptide y) was isolated from the cooh-terminal end of the basic protein of bovine myelin by peptic digestion. this peptide induced experimental allergic encephalomyelitis in the rhesus monkey. treatment of peptide y with cyanogen bromide released three amino acids from the cooh-terminal end and resulted in a tetradecapeptide (peptide m) which was also encephalitogenic in the rhesus monkey. the sequence of peptide m is: phe-lys-leu-gly-gly-arg-asp-ser-arg-ser-gly-ser-pro-met ... | 1975 | 46230 |
| antigenic relationship between the fourth component of human and guinea pig complement. | antigenic similarity between cell-bound human and guinea pig c4 was detected with antisera specific for each of these molecules. no cross-reaction could be detected between c4 of normal guinea pig or human sera or after treatment of the sera with antigen-antibody c1 complexes. the cross-reactivity between cell-bound human and guinea pig c4 may reflect conformational changes in the c4 molecule as a result of binding of the cell surface. | 1975 | 46239 |
| antigen-induced inhibition of experimental allergic encephalomyelitis. iii. localization of an inhibitory site distinct from the major encephalitogenic determinant of myelin basic protein. | defined peptide fragments were isolated from the n-terminal half of the myelin basic protein (bp) molecule and employed for antigen-induced inhibition of experimental allergic encephalomyelitis (eae). guinea pigs pretreated with peptide 44-89, obtained by limited pepsin digestion and purified by column chromatography, were significantly protected against eae subsequently induced by sensitization with bp in complete fruend's adjuvant. peptide 1-20, derived by cyanogen bromide cleavage, did not in ... | 1975 | 46241 |
| characterization of guinea pig mitogenic factor. | guinea pig mitogenic factor (m.f.) was identified in antigen-stimulated guinea pig lymph node lymphocyte cultures by the ability of supernatants to stimulate dna synthesis in non-immune lymphocytes. its activity is not augmented by the presence of antigen and it will stimulate lymphocytes made unresponsive to the eliciting antigen. m.f. production by lymphocytes is detectable within 6 hr after antigen stimulation and reaches a maximum at 24 hr. dna synthesis produced by m.f. is maximal at 48 hr. ... | 1975 | 46245 |
| the antigenicity of myelin encephalitogenic protein: production of antibodies to encephalitogenic protein with deoxyribonucleic acid--encephalitogenic protein complexes. | rabbits immunized with monkey encephalitogenic protein (ep) complexed to deoxyribonucleic acid (dna) formed complement-fixing antibodies that reacted well with ep from monkey, bovine, and human brain and the large ep of rat brain. reactivity with the small ep of rat brain was much less. the antibodies, analyzed by the quantitative micro-complement fixation test, were of the 7s class, were produced in high titer, and failed to react with histone, lysozyme, or acidic liver extracts. immunohistoche ... | 1975 | 46251 |
| complement-mediated alteration of antibody specificity in vivo. | the simultaneous injection of heterologous anti-el4 lymphoma serum and complement results in the rapid disappearance of such antibody from the periphery of non-tumor bearing mice. however, this phenomenon is only observed when a complement source capable of mediating the lysis of el4 cells sensitized with such heterologous antibody is used. this complement mediated enhancement of anti-tumor antibody absorption was observed in vivo for three strains of mice. omission of complement or the use of g ... | 1975 | 46259 |
| application of the paired label radioantibody technique to detection and subtyping of hepatitis b surface antigen. | a sensitive radioimmunoassay has been developed for the detection and subtyping, in regard to w and r specificities, of hepatitis b surface antigens (hbsag). the binding of monospecific rabbit antibodies directed to either w or r determinant, labeled with 131i and 125i, respectively, by hbsag was determined in the same experiment, taking advantage of the fact that these specificities are in general, expressed mutually exclusively. hbsag in the test sample were bound to solid phase guinea pig ant ... | 1975 | 46262 |
| studies on the cytophilic properties of human beta2 microglobulin. | human beta2 microglobulin is cytophilic for mouse, rat, and to a lesser extent guinea pig lymphocytes and polymorphonuclear leukocytes but not for human, rabbit, chicken, frog, and turtle cells, nor for any mature erythrocytes tested. mouse splenic lymphocytes bind 5- 7-fold more beta2 microblobulin than thymocytes although hydrocortisone-resistant thymocytes resemble spleen cells in this regard. the beta2 microglobulin binding by b and t cells in mouse spleen is similar. the structure responsib ... | 1975 | 46266 |
| an attempt to identify the intestinal receptor for the k88 adhesin by means of a haemagglutination inhibition test using glycoproteins and fractions from sow colostrum. | the k88 antigen of escherichia coli specifically adheres to the piglet intestinal cell; a solution of this antigen agglutinates guinea-pig red cells at 4 degrees c. the latter reaction was used as a model of the former, using inhibition of haemagglutination as an index of specific combination with the k88 adhesin. inhibition was found with mucous glycoproteins and chemical modification of their heterosaccharide residues by mild acid hydrolysis, periodate oxidation or the smith degradation proced ... | 1975 | 46271 |
| letter: populations of guineapig lymphocytes displayed by nomarski microscopy. | | 1975 | 46537 |
| chemotherapeutic drugs increase killing of tumor cells by antibody and complement. | when the ascitic forms of two antigenically distinct guinea pig hepatomas induced by diethylnitrosamine are treated in vitro with chemotherapeutic drugs, their sensitivity to killing by xenogeneic antibody plus guinea pig complement increases. the effect is dependent on drug dose, is reversible, and does not appear to be due to increased antigen expression or fixation of the early acting components of guinea pig complement. | 1975 | 46622 |
| formation of cysteinyldopa from glutathionedopa in melanoma. | glutathionedopa injected intravenously into mice is metabolized and excreted in the urine as a compound with the fluorescence characteristics of cysteinyldopa. glutathionedopa incubated with a guinea-pig kidney homogenate is metabolized to a compound with the fluorescence characteristics of cysteinyldopa. boiling of the kidney homogenate prevents the metabolism of glutathionedopa. incubation of glutathionedopa with a homogenate of a melanoma metastasis led to the formation of a compound with the ... | 1975 | 46665 |
| the heart as a target organ in systemic allergic reactions: comparison of cardiac analphylaxis in vivo and in vitro. | the purpose of this investigation was to define and quantitatively evaluate cardiac anaphylaxis in vivo. guinea pigs, passively sensitized with graded amounts of rabbit antipenicilloyl antibody, were anesthetized, ventilated, and challenged intravenously with a constant amount of antigen (anaphylaxis in vivo). in other experiments, guinea pig hearts were excised, perfused in a langendorff apparatus, and challenged (analphylaxis in vitro). during in vivo anaphylaxis, sinus rate increased 10-30 be ... | 1975 | 46793 |
| localization of a protein antigen in the chicken spleen. effect of various manipulative procedures on the morphogenesis of the germinal centre. | the time course of the localization of a protein antigen human serum albumin (hsa) into the chicken spleen after intravenous injection is analysed. localization within seconds to the region surrounding the schweigger-seidel sheaths is accomplished by hsa complexes with chicken anti-hsa or by heat aggregated hsa. the localization of soluble hsa has to await the synthesis of sufficient chicken anti-hsa to accomplish localization to the same white pulp sites in the spleen at 25-30 hours after injec ... | 1975 | 46839 |
| comparsion of the properties of two antigen-induced guinea pig lymphokines. | the properties of macrophage migration inhibition factor (mif) and mitogenic factor (mf) were compared using culture supernatants of antigen-stimulated lymph node cells from inbred guinea pigs. gel filtration on sephadex g-100 indicated molecular weights of about 60,000 and 25,000 for mif and mf, respectively. the lymphokines also differed with respect to heat sensitivity, mif being largely inactivated by 60 degrees c for 20 min, whereas mf was unaffected by this treatment. the time course of pr ... | 1975 | 46849 |
| immune response in the guinea pig to penicillin-autologous carrier proteins. | the spontaneous fixation of penicillin g on guinea pig serum protein was studied. serum albumin and ig were shown to fix firmly over 95% of penicillin. therefore, guinea pigs were immunized with penicillin alone or penicillin-autologous carrier protein conjugates. the production of hemagglutinating antibody, of mif and the in vitro stimulation of lymphocytes were analyzed as immunological parameters. the results showed that in all cases the strongest immunological response was obtained with anim ... | 1975 | 46856 |
| adoptive transfer of experimental allergic encephalomyelitis (eae): prevention of successful transfer by treatment of donors with myelin basic protein. | after onset of experimental allergic encephalomyelitis (eae), guinea pigs can be effectively treated by injection with myelin basic protein (bp). in order to localize the site of action of bp, cells from sensitized donors treated with bp one, two, three, or four times after disease onset have been transferred to normal recipients. one injection of bp has no effect on ability of cells to transfer eae. two injections partially inhibit transfer. after the third and fourth injections the sensitized ... | 1975 | 46879 |
| specificity of basophils and lymphocytes in cutaneous basophil hypersensitivity. | using a rosetting technique, it was found that the vast majority of basophils circulating in the blood or accumulating in the skin reactions of guinea pigs primed for cutaneous basophil hypersensitivity (cbh) lacked demonstrable specificity for sensitizing antigen, whether sheep erythrocytes, a soluble protein, or tumor cells. by contrast, one-third of cells teased from late skin reactions formed specific rosettes as did nearly 80% of circulating basophils in animals receiving repeated doses of ... | 1975 | 46884 |