| the sites of action of 5-hydroxytryptamine in nerve-muscle preparations from the guinea-pig small intestine and colon. | 1 the sites of action of 5-hydroxytryptamine (5-ht) were examined in isolated segments of guinea-pig intestine. mechanical records were taken from the longitudinal muscle of the ileum and proximal colon and from the circular muscle of the ileum and distal colon.2 in order to examine direct actions of 5-ht, nerve-mediated responses were blocked with tetrodotoxin (0.2 mug/ml). there was a gradient in the responsiveness of the longitudinal muscle of the ileum; in the proximal ileum it was usually u ... | 1979 | 32947 |
| beta-galactosidases in mouse duodenal mucosa. | beta-galactosidase activity, in fetal mice, first appears at 16 days of gestation and has a ph optimum of 4. in postnatal development the enzyme activity of cell homogenates tends to show bimodal ph at 4 and 5.6. there are two molecular forms of the enzyme, separable both by molecular-sieve chromatography and electrophoresis. one of the molecular forms of the enzyme is active over a wider range of ph (3.2-6.2) and has half as much activity at 5.6 as it does at 4. this isoenzyme is continuously p ... | 1978 | 33038 |
| the capacity of microsomally-activated cyclophosphamide to induce immunosuppression in vitro. | cyclophosphamide (cy) was activated in vitro with washed rat liver microsomes and cofactors. pretreatment of mouse spleen cells in vitro with the activated drug abolished their capacity to give a primary antibody response to srbc and levan on transfer to irradiated syngeneic recipients. however, responsiveness returned if challenge was delayed for 7 or more days after transfer. part of this was shown to be of donor origin by an allotype marker. the treatment of normal spleen cells with activated ... | 1978 | 33118 |
| palmityl-coa and stearyl-coa desaturase in mouse brain microsomes during development in normal and neurological mutants (quaking and jimpy). | | 1979 | 33235 |
| cytoplasmic malic enzyme from mouse kidneys. | nadp+-dependent cytoplasmic malic enzyme was purified to homogeneity from mouse kidneys by a two-step procedure involving 8-(6-aminohexyl)-amino-2', 5'-adp-sepharose affinity chromatography and deae-sephadex ion exchange chromatography. the biochemical properties of the purified enzyme from dba/2j mice were characterized. these include the determination of molecular weight and amino acid compositions, steady-state kinetics, thermal stability and inactivations by iodoacetate and urea. the native ... | 1978 | 33324 |
| kinetic studies in isolated organs: tools to design analgesic peptides and to analyze their receptor effects. | the opioid activities of peptide and non-peptide narcotics were studied in longitudinal muscle strip of guinea pig ileum (gpi) and in mouse vas deferens (mvd). the comparison of agonist activities of peptides found in gpi and mvd offered the opportunity to predict the presence but not the magnitude of potential analgesic activity. the kinetics of the antagonism between naltrexone and different types of agonists were also determined in these systems. using c-6 epimers of naltrexone, it was found ... | 1979 | 33396 |
| regulation of oxygen affinity in blood of fetal, newborn and adult mouse. | oxygen half saturation pressure (p50) of blood and the role of 2,3 diphosphoglycerate (dpg), adenosine-triphosphate and red cell ph regulating oxygen affinity were examined in fetuses (16,5-18,5 days of gestational age), neonatal (1-22 days post partum) and adult mice (balb/c). the high oxygen affinity of fetal blood (p50 = 29 torr at 37 degrees c, ph 7.4 and pco2 = 40 torr) decreases to an average adult value of 41 torr within two weeks after birth, accompanied by an increase of dpg-concentrati ... | 1978 | 33429 |
| destruction and resynthesis of mouse beta-glucosidases. | 1. injection of a single dose of conduritol b epoxide into mice produced almost complete destruction of glucocerebrosidase (d-glucosyl-n-acylsphingosine glucohydrolase, ec 3.2.1.45) in liver, spleen, brain, and kidney within 5 h. restoration of activity became noticeable within 1 day (2 days in the case of brain) and was about 80% of normal within 16 days. 2. the same injection produced less destruction of aryl beta-glucosidase (ec 3.2.1.21), measured at ph 5.4 with methylumbelliferyl glucoside ... | 1979 | 33720 |
| [effect of some fragments of peptide hormones on the content of biogenic monoamines from mouse brain]. | the effect of some tripeptides, which are fragments of peptide hormones, and their analogs on the content of biogenic monoamines (bm) from albino mice brain was studied. it was found that thyroliberin, melanostatin and the c-terminal tripeptide of gonadoliberin activate the dophaminergic (da-ergic) system in the forebrain of mice treated with reserpine or haloperidol, whereas the c-terminal tripeptide of gastrin acts as a synergic blocker of the da receptors. the n-terminal tripeptides (with and ... | 1979 | 33728 |
| the possible roles of membrane organization in the activity of androgen biosynthetic enzymes associated with normal or tumorous mouse leydig cell microsomes. | the enzymes involved in conversion of pregnenolone to testosterone in leydig cell tumors showed a wide distribution among smooth endoplasmic reticulum (ser), rough endoplasmic reticulum (rer), and cytosol, while these enzymatic activities in normal testes were associated primarily with smooth endoplasmic reticulum. progesterone, used as a substrate in the presence of an nadph-generating system, was metabolized to androstenedione and finally to testosterone by microsomes from some strains of tumo ... | 1978 | 33799 |
| experimental hypoplastic marrow failure in the mouse. | in order to study the pathogenesis of aplastic anemia in man, hemopoietic stem cells were investigated in 'aplastic mice' the aplasia being induced by the immunological method. c3h/he (h-2k, mlsc) received 600 rad whole body x-irradiation followed by the transplantation of 10(7) lymph node cells prepared from b10.br mic e (h-2k, mlsb). the c3h/he mice developed pancytopenia and marrow hypoplasia 21 days after these treatments. the total number of nucleated cells, cfu-s and cfu-c in the marrow an ... | 1978 | 33819 |
| induction of tyrosine hydroxylase and dopamine beta-hydroxylase in cultured mouse neuroblastoma by 8br-camp. involvement of rna and protein synthesis. | | 1979 | 34092 |
| fluroxene mutagenicity. | the commercially available volatile anesthetic fluroxene (2,2,2-trifluoroethyl vinyl ether) which contains the stabilizer n-phenyl-1-napthylamine, was tested for mutagenicity using four strains of s. typhimurium, ta1535, ta1537, ta98 and ta100, and one strain of e. coli, wp2. in addition, purified fluroxene; n-phenyl-1-napthylamine; trifluoroethanol, a major metabolite of fluoroxene; and urine from rats anesthetized with fluroxene were tested. several procedures were utilized including exposure ... | 1978 | 34096 |
| antibodies distinguishing between intact and alkali-hydrolyzed 7-methylguanosine. | antibodies specific for intact 7-methylguanosine (m7g) were induced in rabbits and mice by immunization with nucleoside-bsa or nucleoside-hemocyanin conjugates. since m7g undergoes alkali-catalyzed hydrolytic fission of the purine ring, modifications were made in the procedure for conjugation of m7g to proteins. after periodate oxidation, m7g was incubated with protein at ph 9.1 at 4 degrees c for one hour during which the nucleoside was found to be stable. reduction of the schiff base was done ... | 1978 | 34138 |
| development of guanylate cyclase activity in mouse embryonic lung. | | 1978 | 34309 |
| insulin receptors in cultured mouse retinal cells. | the binding of 125i-insulin to uncloned and cloned cultures of mouse retinal cells has been investigated. at 15 degrees c, binding of the hormone reached a steady state by 60 min, while at 37 degrees c equilibrium was reached earlier but at a lower level than at 15 degrees c. porcine insulin, porcine proinsulin and guinea pig insulin displaced labelled insulin in proportion to their known biological potency. a sharp ph dependence of the hormone binding was observed with an optimum at ph 7.8. the ... | 1979 | 34549 |
| an electron microscopic radioautographic identification of the "enterochromaffin-like" apud cells in murine oxyntic glands. demonstration of a metabolic difference between rat and mouse gastric a-like cells. | | 1979 | 34552 |
| some effects of the administration of endotoxin in mice. specific cleavage of serum albumin by an acid protease and the generation of amyloid serum component. | endotoxin has been shown to induce amyloidosis in mice and to result in the appearance in serum of large amounts of amyloidrelated protein (saa). after injection of 300 mug lipopolysaccharide escherichia coli, saa behaves as an acute phase reactant with levels reaching a peak of >600 mug/ml at 18-22 h and returning to base line (<50 mug/ml) by 48 h in each of four strains tested; only the endotoxin-resistant c3h/hej strain showed a smaller response. lesser, though significant, elevations were al ... | 1979 | 34628 |
| studies on column fractionation of immune cells. vii. electrophoretic mobility and functional behavior of mouse spleen cells after fractionation on a weakly basic ion exchanger. | after passing normal mouse spleen cells through columns of a weakly basic ion-exchanger, the percentage of cells with b cell-typical electrophoretic mobility (epm) is 90% after fractionation at ph 8.0 and 10--20% at ph 5.0. however, the characterization of fractionated cells with regard to theta-antigen as well as graft-versus-host reaction shows that separation of t- and b-lymphocytes had not occurred. after incubation of the separated cells for 2 h at 37 degrees c the epm returned to that of u ... | 1978 | 34959 |
| local graft versus host reaction in the xenogeneic system. | the injection of viable rat spleen cells beneath the renal capsule of cyclophosphamide (cy)-treated adult mice produced a localized graft versus host reaction (gvhr), manifested by the kidney enlargement index (ki). the optimal xenogeneic gvhr was obtained after injection of 30-50 million rat spleen cells into mouse recipients treated 24 hr before with cy, 200 mg/kg intraperitoneally (i.p.), and killed 7 days later. splenomegaly in recipient mice suggested systemic dissemination of the local gvh ... | 1978 | 35123 |
| dna-protein crosslinking by trans-platinum(ii)diamminedichloride in mammalian cells, a new method of analysis. | dna-protien crosslinks produced in mouse leukemia l1210 cells by trans-pt(ii)diamminedichloride were quantitated using the technique of dna alkaline elution. dna single-strand segments that were or were not linked to protein were separable into distinct components by alkaline elution after exposure of the cells to 2--15 kr of x-ray. protein-linked dna strands were separated on the basis of their retention of filters at ph 12 while free dna strands of the size generated by 2--15 kr of x-ray passe ... | 1979 | 35232 |
| inactive 3-hydroxy-3-methylglutaryl-coenzyme a reductase in broken cell preparations of various mammalian tissues and cell cultures. | preincubation of broken cell preparations from a variety of tissues and cell cultures resulted in an apparent increase in the level of 3-hydroxy-3-methylglutaryl-coa reductase activity. however, apparent activation of the reductase in mouse liver, hepatomas and primary liver cell cultures was attributed largely to the loss, during the preincubation period, of an interfering enzyme, 3-hydroxy-3-methylglutaryl-coa lyase. among non hepatic cells and tissues (which did not contain appreciable lyase ... | 1979 | 35236 |
| salicylate blockade of granulocyte adherence and the inflammatory response to experimental peritonitis. | aspirin profoundly inhibited the in vitro augmentation of human and mouse granulocyte adherence to nylon fiber induced by the bacterial products escherichia coli endotoxin and staphylococcus aureus culture filtrate. granulocytes obtained from normal volunteers during the 48 hr following ingestion of aspirin did not respond normally to endotoxin stimulation. furthermore, pretreatment of mice with sodium salicylate prior to intraperitoneal infection with streptococcus pneumoniae impaired granulocy ... | 1979 | 35259 |
| sensitivity of mouse vas deferens to neurotransmitters: changes after morphine treatment. | 1. the pharmacological responses of the isolated vas deferens of the mouse were investigated after acute and chronic treatment with morphine. 2. the addition of morphine to the bath did not alter the responses of the vas deferens to exogenous noradrenaline, adrenaline or dopamine. 3. low doses of morphine depressed the responses to acetylcholine. very high concentrations of the opioid (8.5 x 10(-4) m) completely abolished, in about 50% of the preparations, the responses to exogenous acetylcholin ... | 1979 | 35263 |
| [immunological aspects of nucleolar activation. iii. changes in nucleolar activation after application of s iii pneumococcal polysaccharide in vivo]. | intraperitoneal application of 0.7 microgram s iii pneumococcal polysaccharide led to a significant increase in the values of nucleolar activation in small, medium, and large lymphocytes, plasmoblasts, and plasmatic cells in a white mouse. macrophages did not respond to the application of s iii by an increase in nucleolar activation. the study tested the possibility of using the observation of the functional morphology of the nucleous for observing the changes in the activation of b-cells after ... | 1979 | 35284 |
| studies on disposition and metabolism of tolmetin, a new anti-inflammatory agent, in rats and mice. i. absorption, distribution, and excrection of [14c]tolmetin radioactivity. | [14c]tolmetin was rapidly and almost completely absorbed in both rats and mice. the major portion of the drug was shown to be absorbed from the upper part of the duodenum, and a small portion from the stomach. tissue levels of radioactivity comparable to blood levels were found only in liver and kidney, and other tissue levels were lower than those in blood, possibly because of the considerable plasma protein binding of the drug. radioactivity disappeared from most tissues at rates similar to th ... | 1979 | 35320 |
| studies on disposition and metabolism of tolmetin, a new anti-inflammatory agent, in rats and mice. ii. urinary metabolites. | a new urinary metabolite of tolmetin was found in rat urine and identified as the hydroxymethyl metabolite (tolmetin-oh), an intermediate in the formation of the known carboxy metabolite (tolmetin-cooh). in rats of two strains, the following percentages of the urinary reactivity of [14ia1tolmetin were found in 24 hr: unchanged tolmetin-cooh (58--60%), tolmetin-oh (18--24 %), and its conjugate 6--14%. mouse urine contained the unchanged drug (about 20%), its conjugate and tolmetin-cooh (60%). sim ... | 1979 | 35321 |
| aminomalonic acid and its congeners as potential in vivo inhibitors of l-asparagine synthetase. | aminomalonic acid is a strong in vitro inhibitor of l-asparagine synthetase from leukemia 5178y/ar and from mouse pancreas; the agent is formally competitive with l-aspartic acid (ki = 0.0023 m and 0.0015 m for the tumoral and pancreatic enzymes, respectively). since aminomalonic acid is unstable and inert in vivo as an inhibitor of l-asparagine synthetase, attempts were made to deliver it to the site of its intended action via precursors: the diamide (2-aminomalonamide), the diester (diethylami ... | 1979 | 35346 |
| benzodiazepine receptors: alterations in mutant mouse cerebellum. | | 1979 | 35356 |
| some properties of l-asparagine synthetase of the l-asparaginase-resistant mouse lymphoma 6c3hed [proceedings]. | | 1979 | 35419 |
| immunosupression of the primary and secondary immune response by an igm plasmacytoma (tepc-183). | previously we had established that tepc-183 igm(k) suppressed the primary immune response (ir) to both the t-dependent antigens 2,4-dinitrophenyl-haemocyanin (dnp-hcy) and t-independent pneumococcal polysaccharides. in the current investigation, the effect of tepc-183 on an ongoing immune response to ss-iii and dnp-hcy was examined. it was found that when tepc-183 was injected 6 days after the initial antigen injection, at the height of the primary ir, the response was significantly suppressed t ... | 1979 | 35463 |
| immobilization of neuraminidase and its potential application in the modification of cell surfaces. | vibrio cholerae neuraminidase was immobilized on the inside of 1.0 mm inner diameter nylon tubing with retention of enzyme activity, when assayed at 37 degrees c and ph 5.5 with mucin as substrate. the stabilities of the immobilized and soluble enzymes were similar for up to 3 hr at 37 degrees c. preliminary data indicated that immobilized neuraminidase will release sialic acid from the surface of leukemic akr mouse thymus and spleen lymphocytes; however, the level of immobilized enzyme activity ... | 1979 | 35566 |
| type i interferon induced in mice by infection with trypanosoma equiperdum. | type ii interferon is known to be produced in mice infected with trypanosoma cruzi. therefore, interferon production was investigated in mice infected with trypanosoma equiperdum. interferon was found in the sera of mice infected with t. equiperdum from day 1 after infection until death on day 5. the maximal level of interferon appeared before parasitemia was detected, a finding showing that the endogenous interferon probably had no significant influence on the course of infection. unexpectedly, ... | 1979 | 35574 |
| testicular acid phosphatases of the mouse. characterization after chromatographic fractionation. | the testicular homogenate of adult mice was eluted by deae-cellulose chromatography. four peaks of acid phosphatase were identified. the pooled samples of each peak were used to determine substrate specificity, ph-optima, modifier characteristics, thermal stability, and km-values. the results obtained suggest that each peak may represent a separate and distinct type of acid phosphatase. these observations are very similar to those previously obtained with the rat testis. the acid phosphatase act ... | 1979 | 35719 |
| the effect of antihistamine drugs on the neuroleptic-induced catalepsy. | the effect of atropine on the spiperone- or reserpine-induced catalepsy was compared with the effect pure antihistamines (chlorcyclizine, diphenhydramine, mepyramine) and antiserotonin -- antihistamine drugs (cyproheptadine, danitracen). all the drugs were used in equipotent doses in respect of their central cholinolytic action, assassed previously on the basis of the tremorine test. the potency of the antiserotonin action of chlorcyclizine, diphenhydramine and mepyramine was estimated by assess ... | 1978 | 35784 |
| studies on the effect of the anti-phagocytic agent cytochalasin b on leishmania-macrophage interaction. | mouse peritoneal exudate cells were cultured on coverslips in eagle's basal essential medium. the adhering cells were infected with promastigotes of three different species of leishmania. after 8 h incubation, the macrophages were fixed and stained, and a total of one hundred cells were counted. the rates of infection of macrophages were respectively 53.5 +/- 5% for l. enriettii, 52.3 +/- 5% for l. donovani and 11.7 +/- 2% for l. tropica. when cytochalasin b at concentrations of 2.5, 5 and 10 mi ... | 1979 | 35930 |
| chikungunya virus in vivo autointerference associated with a small plaque variant. | virulent and avirulent plaque variants of chikungunya (chik) virus were compared with the prototype and other strains. interference with infective virus growth or mouse pathogenicity only occurred in the presence of small plaque (sp) variants in homologous systems. possible mechanisms for this are suggested. | 1978 | 35947 |
| differences in the susceptibility of mouse lines to the rickettsial pox agent. | a definite correlation between the susceptibility of spleen macrophage cultures derived from a highly susceptible mouse line (c57bl/6) and a line with low susceptibility (dba/2) and the susceptibility of these mouse lines to infection with rickettsia acari was established. intensive replication of the rickettsiae in cultures from highly susceptible animals caused marked disorders in cell metabolism and eventually death of the culture. cell cultures derived from resistant mice gradually eliminate ... | 1978 | 35948 |
| experimental infection of putorius eversmanni polecats and martes foina martens with tahyna virus. | three putorius eversmanni pole-cats and two martes foina martens aged about 9 months were subcutaneously infected with about 260 suckling mouse ld50 of the extraneurally passaged "236" strain of tahyna virus (california group, genus bunyavirus). viraemia with maximal titres of 1.32 (pole-cats) and 1.28 (martens) dex intraperitoneal (i.p.) mouse ld50/0.02 ml was demonstrated from 48 to 96 hr after inoculation (p.i.). by the plaque-reduction neutralization test, seroconversion was demonstrated 15 ... | 1978 | 35949 |
| detection and tissue localization of components of the immune complex in animals infected and immunized with influenza virus. | mice previously vaccinated or convalescents after infection with mouse-adapted influenza virus were challenged by various routes with live or inactivated virus. the components of the immune complex (antigen-antibody-complement) could be detected by immunofluorescence in lungs, kidneys, and heart. the frequency of positive immunofluorescence reaction was related to serum antibody level and, to some extent, to the route the animals were challenged. | 1979 | 35959 |
| antibody response in man following a small intradermal inoculation with coxiella burnetii phase i vaccine. | a small inoculum (0.2 microgram) of phase i coxiella burnetii vaccine given to individuals previously sensitized to co burnetii elicited a positive skin reaction and a strong igm phase i antibody response as determined by microagglutination, complement fixation and microimmunofluorescence tests. a similar inoculum administered to nonsensitized individuals did not elitic a skin reaction nor stimulate a recognizable antibody response. serum from one of these sensitized and skin tested individuals ... | 1979 | 35962 |
| role of pancreatic l-asparagine synthetase in homeostasis of l-asparagine. | l-asparagine synthetase from mouse pancreas was found to be associated principally with the exocrine pancreas and to be dependent on the age of the animal, but not on gender, diet, or the presence of tumor under the conditions examined. the function of the pancreatic enzyme appears to be to supply l-asparagine for the synthesis of pancreatic proteins. this function is suggested by the high specific activity of l-asparagine in pancreatic proteins after intravenous treatment of bdf1 mice with l-[u ... | 1979 | 35986 |
| studies on the relationship between the degradative rates of proteins in vivo and their isoelectric points. | acidic proteins tend to be degraded more rapidly than neutral or basic proteins in rat liver, skeletal muscle, kidney and brain and in mouse liver and skeletal muscle. we now report a similar relationship among soluble proteins from rat lung, heart and testes, and from human fibroblasts and mouse-embryo cells grown in culture. these findings indicate that the correlation between protein net charge and degradative rate is a general characteristic of intracellular protein degradation in mammals. t ... | 1979 | 36075 |
| characterization of specific differences in protein phosphorylation of the plasma membrane and the endoplasmic reticulum of mouse fibroblasts. | endogenous phosphorylation was studied with highly purified fractions of the plasma membrane and the endoplasmic reticulum of sv40-transformed mouse fibroblasts using [gamma-32p]atp and [gamma-32p]gtp as precursors. with atp maximum overall incorporation of 32p into both membrane fractions occurred at ph 7.8 in the presence of 10 mm mgcl2 after incubation for 1 min. gtp could be utilized only by the plasma membrane fraction showing maximum incorporation of 32p at ph 7.8 and 10 mm mgcl2 after inc ... | 1979 | 36172 |
| technetium-99m-labeled mouse-spleen leukocytes: radiolabeling parameters. | | 1979 | 36355 |
| nuclear labeling in immunoperoxidase studies of mouse tissue as detected by staining at acid ph. | several peroxidase-ig conjugates were applied to sections of fixed mouse tissue. when the peroxidase staining reaction was done at ph 4.5 instead of ph 7.4, a striking reaction on nuclear membrane, chromatin, or chromosomes was observed. this staining was prevented by pretreatment of sections with dnase but not with rnase or after acid elution of histones. it is suggested that at acid ph a redistribution and binding dna of oxidized chromogen or of a chromogen-conjugate complex to dna may account ... | 1979 | 36428 |
| natural, genetically determined resistance toward influenza virus in hemopoietic mouse chimeras. role of mononuclear phagocytes. | radiation chimeras produced by crosswise transfers of bone-marrow cell among histocompatible mice susceptible, or genetically resistant, to lethal challenge by a number of myxoviruses were used to test whether macrophage resistance (as assessed in vitro) and resistance of the animal (as measured in vivo), both previously shown to be brought about by the gene mx, were causally related. 49 chimeras were tested individually, both of resistance of their macrophages to in vitro challenge with m-tur ( ... | 1979 | 36443 |
| the effect of simultaneous administration of 3,4-dihydroxyphenylpyruvic acid and l-dopa on the bioavailability of l-dopa in rat and mouse. | in the rat, administration of 3-(3,4-dihydroxy)-l-phenylalanine (l-dopa) simultaneously with the corresponding alpha-keto acid, 3,4-dihydroxyphenylpyruvic acid (dhppa), gives significantly higher concentrations of l-dopa in the serum and of dopamine and homovanillic acid in the brain than the same dose of l-dopa alone. correspondingly, dhppa potentiates the effect of l-dopa on the locomotor activity in reserpine-treated mice. dhppa is postulated to increase the absorption of intact l-dopa from t ... | 1979 | 36456 |
| hyperactivity response to apomorphine and amphetamine in the mouse: the importance of the nucleus accumbens and caudate-putamen. | | 1979 | 36471 |
| cellular energy metabolism, trans-plasma and trans-mitochondrial membrane potentials, and ph gradients in mouse neuroblastoma. | a method for quantitative evaluation of transmembrane electrical potential and ph gradients across a subcellular compartment in an intact cell is presented. this approach has been applied in studies of mouse neuroblastoma c-1300 clone nb41a3, in which the transmembrane electrical potential and ph gradients and the mitochondrial volume percent have been determined. membrane potentials and ph gradients were measured by two different methods. equilibrium distributions of [(3)h]triphenylmethyl phosp ... | 1979 | 36613 |
| identification, partial purification and biochemical characterization of gamma-glutamyltranspeptidase present as a membrane component in skimmed milk and milk fat-globule membranes, and in mammary-tumour virus from the milk of infected mice. | the enzyme gamma-glutamyltranspeptidase was reproducibly found to be associated with mouse milk particles; it is present in milk fat-globule membranes and mouse mammary-tumour virus of infected swiss mice, also in particles from the milk of uninfected mice. the enzymatic activities observed range among the highest reported for mammalian tissues. the enzyme was partially purified from mouse mammary-tumour virus, and from milk fat-globule membranes. the molecule requires the presence of detergents ... | 1979 | 36916 |
| [effect of presynaptic neurotoxins from the bee and cobra venoms on spontaneous mediator secretion from the motor nerve endings in mice]. | phospholipases a2 (phla) from the bee and cobra venoms induced the three-phasic changes in miniature end-plate potential (mepp) frequency recorded in the mouse diaphragm muscle: an initial fall of transmitter release followed by a transient increase before the final complete blockade. the removal of ca2+ from the perfusing solution (below 10(-9) m) prevented the presynaptic effect of both phla. if all phla molecules were washed out by the ca-free solution, the subsequent exposure to a standard s ... | 1979 | 36937 |
| use of the mouse circling model to demonstrate enhanced striatal actions for oxiperomide and tiapride following denervation [proceedings]. | | 1979 | 36952 |
| genetic control of morphogenetic and biochemical differentiation: lethal albino deletions in the mouse. | | 1979 | 36985 |
| effects of chronic guanethidine on the sympathetic nervous system of mouse and toad. | | 1979 | 37040 |
| differential selectivity of several barbiturates on experimental seizures and neurotoxicity in the mouse. | six barbiturates with diverse time-action characteristics--thiopental, pentobarbital, butabarbital, phenobarbital, diphenylbarbiturate, and barbital--were evaluated for "anticonvulsant" and "neurotoxic" effects. for the former, the mes test, clonic seizures induced by pentylenetetrazol, 90 mg/kg, s.c., and maximal seizures produced by pentylenetetrazol, 200 mg/kg, s.c., were employed. for the latter, we used a rotorod technique. time to peak activity in the mes test was employed as the time for ... | 1979 | 37070 |
| ribosomal phosphoproteins of mouse myeloma cells. changes in the degree of phosphorylation induced by hypertonic initiation block. | | 1979 | 37077 |
| levels of norepinephrine and dopamine in mouse brain regions following microwave inactivation--rapid post-mortem degradation of striatal dopamine in decapitated animals. | | 1979 | 37287 |
| influence of the dopamine-beta-hydroxylase inhibitor fla 63 on the disposition of barbitone in the mouse. | | 1979 | 37309 |
| cobaltous chloride-induced hypothermia. ii: pretreatment with sympathoplegics, antihistamines, and narcotic antagonists. | body temperature depression was noted in rats, mice, and hamsters following intraperitoneal cobaltous chloride administration (25 mg/kg). intracerebral cobalt injection elicited hypotermia in rats and mice but not in hamsters. body temperature depression appeared to be centrally mediated in rats and mice and peripherally mediated in hamsters. the effect of intraperitoneal and intracerebral pretreatment with phentolamine, diphenhydramine, propranolol, cimetidine, and naloxone on the mouse rectal ... | 1979 | 37317 |
| mouse erythropoietic stem cell lines function normally 100 months: loss related to number of transplantations. | marrow stem cell lines from old and young donors in parallel experiments were transplanted into genetically anemic w/wv recipients. these recipients were populated and their anemias were cured by stem cell lines from wcb6f1 or c57bl/6 dorons that had been repeatedly transplanted up to five times at annual intervals into successive w/wv recipients. old marrow cell lines produced erythrocytes normally for as long as 2600 to 3000 days. however, after three to four serial transplantations many stem ... | 1979 | 37377 |
| the regional metabolism of 5-hydroxytryptamine in mouse brain in vitro. | | 1979 | 37396 |
| comparative biochemical studies of isozymes of isocitrate dehydrogenase from the mouse. | | 1979 | 37424 |
| intracellular potassium ion activity in resting and stimulated mouse pancreas and submandibular gland. | intracellular potassium ion activities (aik+) and membrane potentials were measured with double-barrelled, potassium-specific microelectrodes in superfused mouse pancreas and submandibular gland. stimulation with the cholinergic agonist bethanechol caused a marked decrease in aik+ in the submandibular gland, whereas no change in aik+ could be detected in the pancreas. this indicates that bethanechol increases the permeability of the cell membranes to potassium ions in the submandibular gland but ... | 1979 | 37516 |
| inosine may be an endogenous ligand for benzodiazepine receptors on cultured spinal neurons. | mouse spinal neurons grown in tissue culture were used to study the membrane effects of the benzodiazepine flurazepam and the naturally occurring purine nucleoside inosine, which competes for benzodiazepine receptor sites in the central nervous system. application of inosine elicited two types of transmitter-like membrane effects: a rapidly desensitizing excitatory response and a nondesensitizing inhibitory response. flurazepam produced a similar excitatory response which showed cross-desensitiz ... | 1979 | 37602 |
| [linearity of the colony count in mouse spleen and bone marrow]. | | 1979 | 37622 |
| invasion of malignant c3h mouse fibroblasts from aggregates transplanted into the auricles of syngenic mice. | spheroid aggregates of malignant fibroblasts (mo4), shown to be invasive in vitro, were implanted subcutaneously into the auricle of the external ear of syngenic c3h mice. the course of early invasion into the surrounding tissues and the formation of tumours was studied in serial sections of auricles fixed 6 h to 30 days after implantation. mo4 cells are first observed to make contact with the surrounding tissues after 6 h. they exhibit, cytoplasmic extensions and spread from the original aggreg ... | 1979 | 37639 |
| methods of study of the invasion of malignant c3h-mouse fibroblasts into embryonic chick heart in vitro. | methods of study of tumour invasiveness in vitro were investigated using the interactions between malignant virally transformed c3h mouse fibroblasts (mo4) with fragments from embryonic chick heart. invasion of mo4 cells into the heart tissue could be demonstrated in all three-dimensional cultures. on the contrary, seeding of mo4 cells on top of a monolayer of heart cells did not mimic invasion. precultured heart fragments were more suitable for the study of the early phase of invasion than fres ... | 1979 | 37648 |
| survival and immunological responsiveness of rat-mouse bone marrow chimeras. | | 1979 | 37716 |
| mouse kidney trehalase: purification and properties. | trehalase (e.c.3.2.1.28) was isolated from mouse kidney and purified to homogeneity. the enzyme was solubilized with n-octanol and activated by freezing and thawing before precipitation with ammonium sulfate. a 1700-fold purification was achieved by chromatography on deae-cellulose, sp-sephadex, followed by gel filtration on sephadex g-200. only a single form of enzyme activity was shown throughout the fractionation as confirmed by gel electrophoresis of the final preparation. the enzyme was spe ... | 1979 | 37791 |
| [isolation of fab and fc fragments from murine immunoglobulin g1]. | two methods of isolating fab- and fc-fragments from mouse immunoglobulin g1 are presented. the first method involves fractionation of papain protein hydrolysate on a column with deae- (or de-32)-cellulose adjusted with 0.005 m k-phosphate buffer, ph 8. the fab-fragment was eluted from the column with the starting buffer. the fc-fragment was eluted, with the buffer ionic strength being increased to 0.4 m. another method involves protein fractionation on an ion exchanger adjusted with 0.004 m tris ... | 1979 | 37943 |
| the inhibitory effects of presynaptic alpha-adrenoceptor agonists on contractions of guinea-pig ileum and mouse vas deferens in the morphine-dependent and withdrawn states produced in vitro. | 1 isolated ilea from guinea-pigs implanted with morphine pellets were stimulated coaxially, either with or without morphine present in the bath fluid, and the longitudinal contractions recorded. 2 in the absence of morphine the inhibitory effects of the presynaptic alpha-adrenoceptor agonists, clonidine and oxymetazoline were much reduced and the dose-response curve was flat. this state of 'withdrawal' was readily reversed by morphine and levorphanol but not its inactive (+)-isomer, dextrophan. ... | 1979 | 37965 |
| analogs of l-aspartic acid in chemotherapy for cancer. | the interaction of analogs of l-aspartic acid with adenylosuccinic acid synthetase, l-asparagine synthetase, and l-aspartic acid transcarbamylase is discussed. each of these enzymes is of critical importance in the economy of certain types of tumor cells. l-alanosine, a new antitumor antibiotic, is shown to be accepted as a substrate by the enzymes of de novo purine biosynthesis which ordinarily use l-aspartic acid as a substrate; as a consequence of this interaction, an anabolite is thought to ... | 1979 | 38003 |
| cyclic nucleotide metabolism during lymphocyte transformation. ii. comparative studies in the nude balb/c mouse. | | 1979 | 38010 |
| metabolism of 4-hydroxy-2,3-pentene-1-al in submitochondrial fractions of mouse liver. | the location of the enzymic system involved in the metabolism of 4-hydroxy-2-3-pentene-1-al (hpe) in the livers of female mice (cba/ca) has been established. all mitochondrial membranes showed some activity with slightly higher metabolism in the matrix. the enzymic system had a ph optimum of 8.8 using pyrophosphate buffer. suitable marker enzymes were used to test the contamination of the different submitochondrial fractions with each other. | 1979 | 38013 |
| seasonal and social determinants of urinary catecholamines in the lesser mouse lemur (microcebus murinus, cheirogaleinae, primates). | | 1979 | 38043 |
| disposition of flunisolide in the rat, mouse, dog, rhesus monkey, and cynomolgus monkey. | flunisolide (6 alpha-fluoro-11 beta, 16 alpha, 17 alpha, 21-tetrahydroxypregna-1,4-diene-3,20-dione 16,17-acetonide) administered as a single iv or oral dose to rats, mice, dogs, rhesus monkeys, and cynomolgus monkeys had a plasma t 1/2 of 1-3.5 hr and was eliminated mainly via the bile. after iv administration of 14c-labeled flunisolide, radioactivity was widely distributed into tissues and organs. the apparent volume of distribution of flunisolide in these five species was 3.0-8.0 liters/kg. a ... | 1979 | 38078 |
| retention of cytosine arabinoside in mouse lung following intravenous administration in liposomes of different size. | an extrusion technique was used to obtain multilamellar lipid vesicles (mlv, liposomes) of different size distribution. the larger mlv ranged in diameter from 0.1 to 2.6 mu and the smaller from 0.1 to 1.5 mu, and both were composed of phosphatidylserine/phosphatidylcholine/cholesterol in the molar ratio 1:4:5. after intravenous injection of large and small mlv containing encapsulated [3h]cytosine arabinoside (ara-c), their distribution in various organs showed that the fraction of the dose asso ... | 1979 | 38080 |
| chemical synthesis and biological occurrence of carboxylic acid metabolites of delta1(6)-tetrahydrocannabinol. | potential metabolites of (-)-3,4-trans-tetrahydrocannabinol (thc) with a carboxylic acid function in the side chain were synthesized as their methyl esters in the delta1(6)-series. the chromatographic and mass-spectrometric properties of the five side-chain homologues were examined and utilized to facilitate the isolation and the identification of the corresponding monocarboxylic acid metabolites of delta1(6)-thc in the mouse, guinea pig, and rabbit formed after ip administration. the metabolite ... | 1979 | 38086 |
| studies on dehydrogenases of the glucuronate-xylulose cycle in the livers of diabetic mice and rats. | in view of reports that accessory pathways of glucose oxidation are enhanced in the diabetic state, we have determined the levels of key enzymes of the glucuronate-xylulose cycle in the livers of diabetic mice and rats. genetically diabetic mice (db/db) were found to have increased levels of two nadp-linked enzymes of this cycle [nadp-xylitol dehydrogenase and nadp-l-hexonate dehydrogenase (aldehyde reductase ii)], whereas other nad- and nadp-linked dehydrogenase activities of the pathway were n ... | 1979 | 38160 |
| in-vitro differentiation of type a spermatogonia in mouse cryptorchid testis. | cryptorchid testes from adult mice were incubated in calf serum-supplemented medium. there was an effective differentiation of adult type a spermatogonia up to the pachytene stage of meiotid division whcih resembled the process of spermatogenesis in vivo. in the absence of calf serum, type a spermatogonia did not differentiate at all. they differentiated when the serum was present for the first day, but was absent for the rest of cultivation. these results indicate that serum is necessary for on ... | 1979 | 38338 |
| suppression of gvh mortality by mouse neonatal liver cells. | | 1979 | 38544 |
| characterization of rna polymerases from rous sarcoma virus-induced mouse ascites sarcoma cells. | rna polymerase was extracted from the schmidt-ruppin strain of rous sarcoma virus (sr-rsv)-induced c3h/he mouse ascites sarcoma cells (sr-c3h). rna polymerase was separated into rna polymerases i and ii by deae-sephadex chromatography. rna polymerase i was separated into ia and ib fractions by phospho-cellulose chromatography. in sr-c3h cells rna polymerase ib was the main component of rna polymerase i. at 0.05--0.1 m ammonium sulphate rna polymerase i transcribed native dna most actively, and r ... | 1979 | 38635 |
| muscular dystrophy in the mouse: neuromuscular transmission and the concept of functional denervation. | the results of recent investigations by ourselves and others indicate that no form of denervation exists to any remarkable degree in dystrophic mouse skeletal muscles. this conclusion is based on the following information: dystrophic nerve terminals liberate normal amounts of transmitter both spontaneously and during impulse-mediated activity. the characteristics of the release process, the size of the available store of transmitter, and the probability of release of transmitter in response to t ... | 1979 | 38724 |
| inhibitory effects of anticonvulsant drugs on cyclic nucleotide accumulation in brain. | veratridine causes deplorization of excitable cells and produces marked elevation of adenosine 3',5'-monophosphate (cyclic amp) and guanosine 3',5'-monophosphate (cyclic gmp) levels in incubated slices of mouse cerebral cortex. phenytoin, carbamazepine, phenobarbital, primidone, phensuximide, methsuximide, alpha-methyl-alpha-phenylsuccinimide, and high concentrations of clonazepam are anticonvulsant drugs that preferentially prevent maximal electroshock seizures (mes) and generalized tonic-cloni ... | 1979 | 38736 |
| uptake and release of putative neurotransmitters. measurements in regions of the normal and newcastle disease virus-infected mouse brain. | the capacity of various regions of the mouse brain to accumulate a series of putative neurotransmitter compounds has been studied in cerebral homogenates. this uptake is selective, sodium dependent, energy dependent, and exhibits characteristics of high affinity transport. calcium-stimulated release under depolarizing conditions of accumulated radioactive compounds was also examined. large regional variations of uptake and release capacity existed. no clear relation between intensity of uptake a ... | 1979 | 38769 |
| gamma-glutamyltransferase in human and mouse breast tumours. | a series of experimental mouse tumours were assayed for their gamma-glutamyltransferase activities. significantly raised activities were found in a transplantable spontaneous mammary carcinoma compared to normal or lactating mouse breast. a study was therefore undertaken of human breast tumours. carcinomas showed significantly elevated enzyme levels when compared with normal tissue or histologically uninvolved tissue from a breast containing a carcinoma. fibroadenoma and chronic mastitis also sh ... | 1979 | 38923 |
| l-asparaginyl-trna synthetase and l-asparagine synthetase activities of l-asparaginase-sensitive and -resistant forms of the mouse gardner lymphoma 6c3hed. | 1. the mouse gardner lymphoma 6c3hed was grown in ascites fluid in a form sensitive to the action of l-asparaginase (line 1), in another form which was resistant to l-asparaginase (line 2) and in a third form with partial sensitivity to l-asparaginase (line 3). 2. the l-asparaginyl-trna synthetase activities of extracts of the tumour cells, cultured both in the mouse and in vitro, were determined. two of the lines, 1 and 3, in early passage numbers, showed a derepression mechanism involving l-as ... | 1979 | 38936 |
| hallucinogens antagonize histamine h1 receptors of cultured mouse neuroblastoma cells. | hallucinogens were competitive antagonists of histamine at the h1-receptor of cultured mouse neuroblastoma cells. their rank order of potency at this receptor was similar to that for their potency at eliciting subjective effects in vivo. comparison with studies of other receptors, however, suggests that no single model of drug--receptor interaction adequately accounts for the known subjective effects of these compounds. | 1979 | 38972 |
| rapid, large-scale isolation of plasmodium berghei sporozoites from infected mosquitoes. | the discontinuous gradient technique for recovery of malarial sporozoites from mosquitoes (beaudoin et al., 1977) has been modified to speed up recovery and prevent sensitization of mice by components of the gradient which contaminate the sporozoites used as antigen. mouse serum was substituted for bsa in the gradient because the latter produced hypersensitivity. best results were obtained with gradients consisting of medium 199, renografin and mouse serum. heavy and light solution of gradient c ... | 1979 | 39127 |
| pancreatic acinar cells: the effect of carbon dioxide, ammonium chloride and acetylcholine on intercellular communication. | 1. segments of mouse pancreatic or exorbital lacrimal gland were superfused with saline solutions. under visual control two micro-electrodes were inserted into neighbouring cells within the same acinus or into neighbouring acini. cell to cell electrical coupling was assessed by injecting rectangular current pulses through one electrode and measuring the electrotonic potential change in the same cell (v(1)) and in the neighbouring cell (v(2)). acetylcholine (ach) was added locally to impaled acin ... | 1979 | 39163 |
| dose-related response of mouse mamma to some phenothiazine drugs. | the dose-related response of the mammary growth was studied in immature icr-jcl female mice given 0.03 micrograms of estradiol per animal subcutaneously, and 0.005-1.0mg of either of the following phenothiazines (chlorpromazine, levometh iomeprazine, promethazine, perphenazine, thioridazine and propericiazine) per 10g of body weight was given orally once a day for 9 days starting on the 5th day after ovariectomy. the right second mamma of the chest was used to compare the hypertrophic affects. a ... | 1978 | 39177 |
| tricyclic antidepressants and histamine h1 receptors. | tricyclic antidepressants and some structurally related compounds were tested for their ability to antagonize histamine h1 and muscarinic acetylcholine receptors of cultured mouse neuroblastoma cells. as a group, tertiary amine tricyclic antidepressants tended to be more potent than secondary amine drugs at both receptors. the most potent antihistamine, doxepin hydrocholoride, was about 4 times more potent than amitriptyline hydrochloride, about 800 times more potent than diphenhydramine hydroch ... | 1979 | 39202 |
| investigations on the mechanism of cyclic guanosine monophosphate increase due to depolarizing agents as studied with sea anemone toxin ii in mouse cerebellar slices. | sea anemone toxin ii (atx ii) and mcd-peptide, like other depolarizing agents, raise the content of cgmp and to a lesser extent of camp in mouse cerebellar slices. na+ influx and ca2+ movement are involved in their mode of action, as indicated by the following observations: 1. the rise of cgmp due to atx ii, mcd-peptide and high potassium was diminished when na+ had been replaced by li+. 2. the effects of both toxins and veratridine, but not of high potassium stimulation were prevented by tetrod ... | 1979 | 39260 |
| characterization of two chikungunya virus variants. | the stability of virulence and plaque size of two variants of chikungunya virus (genus alphavirus) were examined. mouse pathogenicity did not coincide with infective virus levels in the brain. an increase in the average survival times of mice receiving a lethal dose of the variant which allowed prolonged survival at high doses and harvested late after infection was observed. studies in vero cells suggested that the number of infective virus particles produced per cell was lower with the small pl ... | 1979 | 39435 |
| long-term culture of guinea pig tongue cells: a suitable interferon system. | the interferon system was investigated in a long-term culture of guinea pig tongue (gpt) cells. interferon synthesis was induced with newcastle disease virus (b strain) and f2 phage double-stranded rna. guinea pig interferons were about 2 times more active in cell cultures established from guinea pig embryos than in the gpt cells. human leukocyte and mouse peritoneal cell interferons were active in this cell system also. | 1979 | 39441 |
| [on the narco-hypnotic of o-chloro 2-phenyl ethyl allophanate (s. c. s. 100) in animal (author's transl)]. | oral administration of o-chloro 2-phenyl éthyl allophanate (s. c. s. 100) to male and pregnant, or not, female mice, to male and female rats, to male guinea-pigs and rabbits induces, according to tested doses and animal species, a reduction of motoricity, a state of sleepiness giving way to exterior solicitations, a loss of balance, a diminution of muscular tonus or a temporary loss of righting reflex going with narcosis and a reversible suppression of sensibility. the narco-hynoticaction of s. ... | 1979 | 39471 |
| partial purification and properties of l-asparagine synthetase from mouse pancreas. | l-asparagine synthetase was partially purified from mouse pancreas to a final mean specific activity of 0.10 unit/mg of protein. the enzyme exhibited an l-glutaminase activity which was not affected by l-asparate, nh(4)cl, atp-mgcl(2), l-glutamate, amp (sodium salt) or sodium pyrophosphate. the l-glutamine-dependent l-asparagine synthetase activity of the partially purified enzyme from mouse pancreas was markedly decreased by freezing for 7 days at -87 degrees c in the presence of 1mm-dithiothre ... | 1979 | 39555 |
| effects of 6-(o-chlorophenyl)-8-ethyl-1-methyl-4h-s-triazolo [3,4-c]thieno[2,3-e][1,4]diazepine (y-7131) on the metabolism of biogenic amines in brain. | a new derivative of thienodiazepine, 6-(o-chlorophenyl)-8-ethyl-1-methyl-4h-s-triazolo[3,4-c]thieno[2,3-e][1,4]diazepine (y-7131) decreased the turnover of 5-hydroxytryptamine (5-ht) but not that of dopamine (da) in the rat brain. the potency of y-7131 in inhibiting the 5-ht turnover was stronger than that of diazepam. imipramine decreased the turnover of 5-ht, whereas chlorpromazine increased the turnover of da. y-7131 decreased the turnover of norepinephrine (ne) in the mouse brain. with diaze ... | 1978 | 39571 |
| effects on induction of tyrosine aminotransferase in fetal mouse liver in vitro of prednisolone, insulin and thyroxine. | the hormonal requirements for formation of tyrosine aminotransferase (ec 2.6.1.5) in fetal mouse liver were investigated in organ culture using chemically defined medium. the hormones tested were insulin, thyroxine and prednisolone. prednisolone alone resulted in a two-fold increase in tyrosine amino-transferase activity in explanted liver in hormone-free medium on day 6, and its effect was dose dependent, but neither insulin nor thyroxine alone induced the enzyme. addition of prednisolone plus ... | 1979 | 39632 |