| studies on bovine leukaemia. iii. the haematological and serological response of sheep and goats to infection with whole blood from leukaemic cattle. | | 1976 | 186987 |
| spectrin rearrangement early in erythrocyte ghost endocytosis. | the endocytic vacuoles induced in white ghosts were found to be depleted of spectrin and therefore it was proposed that they arose from spectrin-free areas in the erythrocyte membrane. to follow changes in spectrin distribution during endocytosis, affinity-purified rabbit antispectrin antibodies were produced. quantitative techniques were developed for the use of a highly specific 125i-f(ab')2 antispectrin, and these showed that before the appearance of vacuoles, as assessed by phase microscopy, ... | 1979 | 117012 |
| ontogenesis, distribution and relative sensitivity of hcg receptors to hcg and lh in goat ovaries. | the binding of [125i]hcg to immature and mature follicles and corpora lutea of goat ovaries has been studied. the hormone is bound maximally by corpora lutea although mature follicles also exhibit some binding. immature follicles are practically devoid of receptors for this hormone. in the corpus luteum, the receptors for the hormone are present in thecal and luteal cells. autoradiographic studies show the location of the bound radioactivity grains primarily along the plasma membranes of these c ... | 1976 | 187505 |
| lasar flow cytophotometric immunoperoxidase detection of herpes simplex virus type 2 antigens in infected cultured human cells. | human cells in culture (hep-2) were infected with herpes simplex virus type 2 (hsv-2) at multiplicities of infection varying from 0.2 to 10, and fixed 6, 12, 18 and 24 hr after infection. infection-related antigens were detected by an indirect double antibody (peroxidase conjugated goat anti-rabbit to rabbit anti-herpes simplex virus type 2) immunoenzymatic staining reaction that rendered infection-related antigens visible by light microscopy. a corresponding series of laser flow cytophotometric ... | 1976 | 187690 |
| diffusion in gel-enzyme linked immunosorbent assay (dig-elisa): a simple method for quantitation of class-specific antibodies. | a new method for quantifying class-specific antibodies is presented. the method has been named diffusion-in-gel-enzyme-linked-immunosorbentassay (dig-elisa), and is briefly as follows. antiserum ia allowed to diffuse from wells in a gel layered over an antigen-coated plastic surface. the gel is then removed and the preparation is incubated with enzyme-conjugated anti-immunoglobulin. the enzyme is then visualised in situ by a colour reaction produced by pouring a substrate-containing gel over the ... | 1979 | 117055 |
| isolation and purification of bovine igm by dissociating immunoglobulin-brucella complexes. | methods for the dissociation of immunoglobulin-brucella complexes (igm-rich) were evaluated. the antigen-antibody complex was separated from serum by centrifugation and dissociated with either h2o, 2.0 m ph 2.0 glycine buffer, or various concentrations of kscn. optimal antibody recoveries for kscn were obtained using 1.0--2.0 m kscn. dissociation of the complex with water resulted in the highest recovery of antibody and the glycine buffer was lowest. immunoelectrophoretic analysis of the eluted ... | 1979 | 118220 |
| cyclic nucleotide immunocytochemistry. | | 1976 | 188313 |
| corticotroph cells in primary cultures of rat adenohypophysis: a light and electron microscopic immunocytochemical study. | monolayer cultures of trypsin-dispersed cells of the rat adenohypophysis were grown for 5 to 54 days. acth was localized by immunocytochemistry using an antiserum to synthetic acth1-28 prepared in rabbit and sheep anti-goat immunoglobulin coupled with peroxidase. acth content of the culture medium was measured by radioimmunoassay. corttion time. the corticotrophs retained their essential morphological characteristics. immunological staining was found in the secretory granules, some tubular or sa ... | 1976 | 188791 |
| experimental milk-borne transmission of powassan virus in the goat. | a lactating goat with a 74-day-old kid was inoculated with 10(3) mouse 50% lethal dose (ld50) of powassan virus. no ensuing viremia could be detected, but virus was secreted in the milk on postinoculation days 7 through 15, with a titer of 10(5) ld50/ml on day 12. neutralizing antibody was found in the serum on days 22 through 36 and in the milk on day 36. the offspring was not inoculated but was allowed to continue feeding on its mother's milk. it developed neutralizing antibody by day 22. sinc ... | 1977 | 190910 |
| cellular localization of ligandin in rat, hamster and man. | | 1977 | 191017 |
| suppressor cells in tumor-bearing mice capable of nonspecific blocking of in vitro immunization against transplant antigens. | spleen cells from mice with progressively-growing methyl-cholanthrene-induced tumors, when immunized in vitro against transplant alloantigens, developed less cytotoxic activity against these antigens as measured by a short-term chromium-release assay than did spleen cells from normal mice. the hyporesponsiveness of spleen cells from the tumor-bearing mice seemed to be due to the presence of suppressor cells which could be removed by nylon-column passage but not by anti-theta treatment and which, ... | 1975 | 123545 |
| in vitro metabolism of red blood cells from newborn and adult goats. | the erythrocytes of newborn and adult goats were studied with respect to gsh stability, gsh regeneration, levels of atp and 2, 3-dpg, glucose consumption and activities of nine different enzymes of embden-meyerhof and pentose phosphate pathways of glucose metabolism. red cells from newborn goats had significantly higher levels of atp and 2, 3-dpg. the activities of all the enzymes measured were also significantly greater in the newborn. it is suggested that newborn goats possess red blood cells ... | 1976 | 129176 |
| [aujeszky's disease in goats (authors transl)]. | the small number of cases of aujeszky's disease in goats referred to in the literature is stressed, and an outbreak in goats causing many deaths is reported. the flock of goats concerned was occasionally housed in a barn during the night in which there also were fattening pigs. the first deaths occurred without previous symptoms, the animals being found dead in the morning. in subsequent cases, symptoms were observed which continued for a few or several hours and consisted in agitation, lying do ... | 1977 | 191954 |
| the regulatory principles of glycolysis in erythrocytes in vivo and in vitro. a minimal comprehensive model describing steady states, quasi-steady states and time-dependent processes. | a simple mathematical model for glycolysis in erythrocytes is presented which takes into account atp synthesis and consumption. the system is described by four ordinary differential equations. conditions in vivo are described by a stable steady state. the model predicts correctly the metabolite concentrations found in vivo. the parameters involved are in agreement with data on the separate steps. the metabolite changes found in pyruvate kinase-deficient erythrocytes and the species variations am ... | 1976 | 132930 |
| induction of parturition in farm animals. | | 1976 | 192122 |
| detection by indirect immunofluorescence of fc receptors in cells acutely infected with herpes simplex virus. | in attempting to use the indirect fluorescent antibody test (ifa) to measure antibodies to herpes simplex virus (hsv), we found that all human sera gave a positive reaction with chang liver cells infected with type 1 (hsv). all sera gave equivalent titers of 320-640 for acetone-fixed cells and about 40 for live cells (membrane fluorescence) in the presence of fluorescein-labeled antisera to human ig; none of the sera reacted with uninfected cells. the fluorescence seen in fixed cells was primari ... | 1977 | 192682 |
| recent data on the properties of the viruses of kuru and transmissible virus dementias. | | 1975 | 134597 |
| staphylococci from the feces of different animal species: biotypes of staphylococcus aureus strains of sheep and goat origin. | staphylococcus aureus was found in 96% of the rectal swabs from 133 sheep and in 80% of the swabs from 125 goats. seventy-seven percent of the isolates from both hosts exhibited the fibrinolytic and growth characteristics of human biotype a on human plasma and crystal violet agar, respectively, but 99% of these isolates resembled s. aureus of animal origin in their other properties. only 21% of the sheep and 24% of the goat isolates were clearly identifiable as human biotype a and animal biotype ... | 1976 | 135527 |
| allergy in cattle after foot-and-mouth disease vaccination. | bhk cell derivatives, saponin and aluminium hydroxide, all of which are commonly present in foot-and-mouth disease vaccines, were injected subcutaneously into 10 cattle and the serum levels of anti-bhk reagins and passive haemagglutinating antibodies were followed. the reaginic antibodies rose to a peak titre one to two weeks after the stimuli and waned during the third week. they had a serum half life of about three days, assuming exponential decay, and were generally undetectable four weeks af ... | 1977 | 193234 |
| effects of estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate on catalytic activity, amount, and rate of de novo synthesis of hepatic histidase. | the mechanisms by which estrogen, glucocorticoid, glucagon, and adenosine 3':5'-monophosphate (camp), regulators which participate in the postnatal development of rat liver histidase, elevate the catalytic activity of this enzyme have been explored. a monospecific antibody against homogeneously purified preparations of rat liver histidase has been elaborated in the goat. employing this antibody in immunotitration experiments, it has been demonstrated that the elevations of hepatic histidase acti ... | 1977 | 193832 |
| t-and b-lymphocyte chimerism in the marmoset. | marmosets are natural blood chimeras, this condition resulting from the high frequency of fraternal twinning and the consistent development of placental vasular anastomoses between the two embryos. identification of chimerism by sex-chromosome analysis of cultured blood lymphocytes provided a means of determining the proportion of chimerism among t and b lymphocytes. peripheral blood lymphocytes were enriched for t or b cells by filtration through a nylon column (yields greater than 95 per cent ... | 1977 | 139360 |
| passive immunity to feline leukemia: evaluation of immunity from dams naturally infected and experimentally vaccinated. | antibodies against feline leukemia virus (felv) and the feline oncornavirus-associated cell membrane antigen (focma) were transferred from pregnant cats to their suckling kittens. all of these kittens were protected against infection and oncogenesis by virulent felv when challenged at 2 weeks of age. suckling kittens acquired 25 to 100% of maternal virus-neutralizing and focma titers by 3 days of age, and titers underwent linear decay to undetectable levels by 2 to 3 months of age. focma antibod ... | 1977 | 194840 |
| evidence for three enzymatic activities in one electrophoretic band of 3-phosphoglycerate mutase from red cells. | electrophoresis of 3-phosphoglycerate mutase from erythrocytes of man and several animal species has been performed on cellulose acetate strips. in most cases the electrophoretic pattern of this enzymatic activity shows three bands. 2,3-diphosphoglycerate phosphatase and diphosphoglycerate mutase from erythrocytes of the same species have been revealed after migration during the same electrophoresis. we found that the band of 2,3-diphosphoglycerate phosphatase and the band of diphosphoglycerate ... | 1975 | 177097 |
| some properties of the goat placental lactogen. | goat placental lactogen was partially purified from a medium collected after placental tissue incubation. the data obtained by disc electrophoresis and isoelectric focusing experiments, as well as by means of radioreceptor assay methods, provide evidence of the similarity between the goat and ovine placental lactogen. | 1977 | 196883 |
| laser cytophotometric detection of variations in spatial distribution of concanavalin-a cell surface receptors following viral infection. | human cells in culture (h.ep. 2) were infected with herpes simplex virus type 2 at a multiplicity of infection (m.o.i.) of 3 for 3, 18 and 36 hr. spatial redistribution of concanavalin (con-a) cell surface receptor sites was detected by an indirect double-antibody (peroxidase-conjugated goat anti-rabbit to rabbit anti-con-a) immunoenzymatic staining reaction which rendered the con-a sites visible to light microscopy and to flow laser cytophotometry. significant spatial redistribution of cell sur ... | 1977 | 197163 |
| infection of british deer and farm animals with epizootic haemorrhagic disease of deer virus. | | 1977 | 198436 |
| effect of pregnancy and lactation on triglycerides of very-low-density lipoproteins of rat plasma. | triglyceride in the blood of rats increased during pregnancy, decreased to control during lactation, and increased again on weaning. the triglyceride content of the very-low-density lipoproteins (d less than 1.006 g/ml) changed in parallel with that of the plasma, and its magnitude indicated that it was chiefly responsible for the transport of triglycerides in the blood. these changes were accompanied by changes in the electrophoretic pattern of the lipoproteins of rat plasma, but no such change ... | 1976 | 178703 |
| [model studies on virus-induced tumors and their immunological treatment (author's transl)]. | after a review of the general biological properties of c-type oncorna viruses, results are presented on the structure of an exogenous murine leukemia virus (flv) and on the serobiological properties of its structural proteins. our findings suggested a major role of the viral surface glycoprotein gp71 in immunological defense mechanisms. this was confirmed by vaccination experiments with isolated gp71 in mice. the induced immunity was highly specific and not operative against endogenous murine c- ... | 1977 | 198602 |
| [quantitative indices and physico-chemical properties of non-specific inhibitors of influenza a virus hemagglutination in the sera of different species of animals and birds]. | the results of studies of the quantitative values of nonspecific antihemagglutinins of influenza viruses in the sera of birds, laboratory, wild and domestic animals (altogether 27 species) are presented. the antiviral inhibitors characterized by a number of physicochemical properties (sensitivity to heating, kio4, trypsin, rivanol, 2-mercaptoethanol) were divided into 3 groups, sera of sheep, goats and cattle making up a separate group with regard to their sensitivity to heating and treatment wi ... | 1977 | 198965 |
| [15 new serotypes of salmonella of subgenus i isolated in africa]. | filteen new serotypes of salmonella belonging to the sub-genus i have been isolated in africa. isolated in ivory coast: s. dabou (8,20:z4,z23,l,w), s. elokate (9,12:c:1,7), s. assinie (3,10:1,w:z5), s. anna (13,23:z35:e,n,z16), s. mpouto (16:m,t:-), s. banco (28:r:1,7), s. abidjan (38:b:l,w); in senegal: s. bignona (17:b:e,n,z15), s. lode (17:r:1,2), s. derkle (52,e,h:1,7); in tchad: s. moussoro (1,6,14,25:i:e,n,z15), s. drogana (1,4,12,27:r,i:e,n,z15); in algeria: s. hydra (21:c:1,6); in haute- ... | 1976 | 199365 |
| negative water balance induced by intracerebroventricular infusion of deuterium. | | 1976 | 179281 |
| studies on the occurrence and distribution of hi antibodies against some arboviruses in the serum of domestic mammals in puglia. | the virological and serological studies previously carried out on arboviruses in italy are reviewed. the presence of antibodies to 11 arboviruses was investigated in the serum of various domestic animals (100 horses, 107 pigs, 102 sheep, 205 goats, 100 cattle and 200 dogs) from some areas of puglia. the techniques are described. the results, given in tables and discussed in detail, support the hypothesis that in this region also there are arboviruses circulating, particularly those of group b. | 1977 | 201546 |
| experimental infection of nigerian sheep and goats with bovine virus diarrhoea virus. | apart from leucopenia and a low grade pyrexia nigerian goats showed no ill effects following inoculation with bovine virus diarrhoea virus. sheep showed a variable leucopenia without pyrexia. no difficulty should arise in differentiating bvd infections from peste des petits ruminants. | 1977 | 202049 |
| urinary excretion of retinol-binding protein (rbp) in rabbit chronically poisoned with cadmium. isolation and partial characterization of rabbit rbp. | studies were conducted to induce urinary excretion of retinol-binding protein (rbp) in the rabbit by long-term administration of cadmium, and to isolate and characterize rabbit rbp. two rabbits were exposed to cd at a dose of 0.8 and/or 1.5mg/kg of body weight by means of subcutaneous injections five times per week. one rabbit excreted large amounts of protein (0.8 to 1.6 g/day) from days 80 to 118. the electrophoretic patterns of the urinary protein showed two fluorescent bands specific for pro ... | 1976 | 179990 |
| specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte. | highly purified preparations of hemin-controlled repressor of rabbit reticulocyte contain a 3':5'-cyclic amp-indenpendent protein kinase activity that phosphorylates the low-molecular-weight (about 38,000) polypeptide chain of the initiation factor that forms a ternary complex with gtp and met-trnaf. these preparations also phosphorylate several polypeptide components of reticulocyte 40s ribosomal subunits. however, no significant levels of phosphorylation are observed when casein, histones, art ... | 1976 | 184458 |
| the isolation of adenoviruses from goats affected with peste des petits ruminants in nigeria. | mucosal scrapings from the large intestine of two goats that had died from peste des petits ruminants (ppr) in separate outbreaks in nigeria were examined for viruses. a mixed viral infection of ppr virus (morbillivirus) and adenovirus was confirmed in both goats. the adenoviruses did not conform to any of the ovine and bovine serotypes recognised; the two isolates were considered different serotypes. it is concluded that, although the role of adenoviruses in the epizootiology of ppr in nigeria ... | 1977 | 203986 |
| an immunofluorescence diagnostic test for feline viral rhinotracheitis. | hyperimmune serum against feline viral rhinotracheitis was produced in a goat and conjugated with a fluorescent dye. cell cultures infected with rhinotracheitis virus had positive immunofluorescence. cell cultures infected with other feline viruses and herpesviruses of other species did not fluoresce. in cats experimentally infected with rhinotracheitis virus, the virus was isolated from nasal and conjunctival swabs 1 to 9 days after inoculation. nasal smears stained with the conjugated antiseru ... | 1978 | 205147 |
| pulmonary angiotensin-converting enzyme antienzyme antibody. | a method has been developed for quantitating anticatalytic activity in antibody preparations made in goats against pure solubilized angiotensin-converting enzyme from rabbit pulmonary membranes. anticatalytic activity was purified about 90-fold from a single batch of serum by a procedure including diethylaminoethylcellulose chromatography and elution from sepharose columns containing covalently bound pure enzyme. antiholoenzyme antibody was fractionated with respect to charge and binding affinit ... | 1976 | 186101 |
| the antibody response in sheep infected with a kenyan sheep and goat pox virus. | | 1978 | 207747 |
| identification of murine leukemia viral antigens detected on mouse cells by h-2 alloantisera. | h-2 alloantisera have been previously reported to contain antibodies against murine leukemia viral antigens, but the nature of the viral antigens on mouse cells which interact with these antibodies has not been established. we have found that h-2 alloantisera recognize components of molecular weight 70 000-80 000 mouse lymphocytes and leukemia cells. these components were also detected by a goat antiserum against the murine leukemia virus (mulv) glycoprotein (gp 70) and are therefore closely rel ... | 1976 | 187431 |
| an epr study of structural perturbations induced by methylindole in the protein and lipid regions of erythrocyte membranes. | 3-methylindole has been shown in previous work to cause pulmonary edema and emphysema in cattle and goats. in this paper, evidence is presented to show that 3-methylindole induces structural perturbations in bovine erythrocyte membranes. the structural perturbations which were induced as a function of 3-methylindole concentration in the membranes were measured by epr using the attachment of maleimide spin label to the sulfhydryl groups of membrane proteins and by intercalation of methyl-5- doxyl ... | 1975 | 164912 |
| suppressed murine mammary tumor virus (mumtv) expression in riii female mice treated neonatally with goat antiserum to mumtv. | passive immunization of newborn inbred riii (r3) mice with the globulin fraction of goat antiserum to murine mammary tumor virus (mumtv) successfully suppressed mumtv expression in the milk of some of the treated mice throughout nine successive lactations. no mammary tumors developed in the mumtv-suppressed mice during the first 9 months, whereas all untreated r3 female breeders expressed mumtv in the milk of the third lactation, and all developed tumors before 9 months of age (mode and median: ... | 1978 | 210290 |
| the incidence of antibody to infectious bovine rhinotracheitis virus in kenyan cattle. | a total of 3204 cattle sera, collected between 1966 and 1974, from all seven provinces and 34 of the 42 districts of kenya were screened in a neutralisation test for antibody to infectious bovine rhinotracheitis virus (ibrv). antibody to ibrv was found in some sera from all the districts, with cattle over two years old showing the highest incidence. a small number of goat sera also showed some antibody. from the results obtained it is concluded that ibrv infection is widespread in kenyan cattle. | 1975 | 165572 |
| an electron microscopic method for the mapping of proteins attached to nucleic acids. | an electron microscopic method for demonstrating the presence of and mapping the positions of proteins specifically bound to nucleic acids is described. the nucleic acid-protein complex is treated with dinitrofluorobenzene under conditions such that dinitrophenyl (dnp) groups are attached to nucleophilic groups on the protein, with only a low level of random attachment to the nuclei acid. this product is treated with rabbit anti-dnp igg. the position of the protein-(dnp)n(igg)m complex on the nu ... | 1978 | 211486 |
| rotavirus in goats. | | 1978 | 211701 |
| ca2+-dependent regulator. production and characterization of a monospecific antibody. | | 1978 | 212426 |
| possible windborne spread of bluetongue to portugal, june-july 1956. | the possible sources for the epidemic of bluetongue in portugal at the beginning of july 1956 were examined. introduction through authorized importation of domestic or wild ruminants was not feasible, since no cattle, sheep or goats were imported and the wild ruminants were confined to lisbon zoo, which was too far from the initial outbreaks. weather maps were examined to see if the wind could have carried infected culicoides midges from north africa. on 21 june 1956 infected midges in morocco c ... | 1978 | 212475 |
| [comparison of various serological methods for the demonstration of maedi/visna virus antibodies]. | | 1978 | 212904 |
| suppression of chemical (den) carcinogenesis in swr/j mice by goat antibodies against endogenous murine leukemia viruses. | | 1978 | 213784 |
| radioimmunoassay of human high density lipoprotein apo-protein a-1. | a double antibody radioimmunoassay technique was developed for the measurement of apolipoprotein a-i, the major apoprotein of human high density lipoproteins. apolipoprotein a-i was prepared from human delipidated high density lipoprotein (d equal to 1.085-1.210) by gel filtration and ion-exchange chromatography. purified apolipoprotein a-i antibodies were obtained by means of apolipoprotein a-i immunoadsorbent. apolipoprotein a-i was radiolabeled with 125-i by the iodine monochloride technique. ... | 1975 | 166665 |
| transport of dietary cholesterol into blood and milk of the goat. | position 4 labeled carbon-14 cholesterol was placed in abomasums (stomachs) of two lactating goats. blood and milk samples were collected from the animals for 5 to 13 days. specific activities of cholesterol and cholesteryl esters in various fractions of blood serum and milk were determined to reveal pathways by which dietary cholesterol enters milk. results with the two animals showed similar trends. within 24 h both cholesterol and cholesteryl esters of the three principal serum lipoproteins o ... | 1975 | 167066 |
| hormonal control of parturition in the sheep and goat. | | 1978 | 216120 |
| [enterotoxemia in goats]. | | 1978 | 216132 |
| [visna in the goat]. | | 1978 | 216191 |
| an immunoprecipitin study of the incidence of influenza a antibodies in animal sera in the ottawa area. | a survey of over 600 'normal' sera from 14 animal species by immunoprecipitin tests in cellulose acetate using viron antigens revealed a high incidence of precipitating activity against a broad range of influenza a virus strains, particularly a2hhong kong/1/68 and /pr8. however, serum treatments trypsin-heat-periodate, naio4, v. cholerae receptor-destroying enzyme (rde), or kaolin eliminated most precipitating activity, which suggests that it was due to "non-specific" inhibitors of influenze vir ... | 1975 | 167931 |
| pathogenicity of a caprine herpesvirus. | a herpesvirus isolated from neonatal angora kids (capra hircus) with a relatively severe generalized infection was shown to be infective for adult goats as well as for kids. however, the virus lacked pathogenicity for either lambs or calves. although a nonreciprocating serologic overlap exists between the caprine virus and the antibody to bovine herpesvirus (bhv-1), results of cross immunity tests in calves indicated that the 2 viruses are immunologically distinct. on the basis of these findings ... | 1975 | 173212 |
| research--the lifeline of veterinary medicine. | | 1978 | 216897 |
| the identification and monitoring of cell line specificity. | | 1978 | 218226 |
| enhanced antiglobulin-mediated neutralization of herpes simplex virus-igg complexes by complement and heterologous anti-immunoglobulin. | the ability of igg anti-fc and anti-fab to neutralize infectious herpes simplex virus-igg (hsv-igg) complexes was determined. when limiting amounts of antiglobulin were used, antibody directed against the fab portion of human igg was significantly more effective than anti-fc antibodies in neutralizing the hsv-igg complexes. the detection of viral bound antibody was enhanced by the incorporation of heterologous antiglobulin or complement in the antiglobulin neutralization test. specifically, hsv- ... | 1979 | 218539 |
| investigations on the ability of antisera produced by pr-beta-hcg-tt to neutralize the biological activity of hcg. | | 1976 | 173494 |
| cell membrane antigen isolation with the staphylococcal protein a-antibody adsorbent. | procedures are detailed for the rapid isolation of representative cell membrane antigens with protein a-bearing staphylococci as an adsorbent for igg antibodies complexed with the antigens. cell surface membrane proteins were radioiodinated and solubilized in nonionic detergent. specific antisera were subsequently added and the immune complexes precipitated by addition of the staphylococcal adsorbent and low speed centrifugation. the antigens isolated included surface immunoglobulins from mouse ... | 1976 | 187693 |
| [hypothalamic neurotransmission processes in the release of antidiuretic hormone in goats]. | | 1978 | 219634 |
| modifications of the immunofluorescence assay for feline leukemia virus group-specific antigens. | observations and minor modifications are presented concerning the immunofluorescence assay for feline leukemia virus (felv) group-specific antigens (gsa) in blood cells of cats. data are given regarding absorption of goat felv gsa antiserum in vivo in cats, absorption of the antiserum in vitro with feline blood cells, and the comparative efficacy of various chemical fixatives in preservation of felv gsa for immunofluorescent staining. the best results were obtained with in vitro absorption of an ... | 1978 | 219732 |
| purification and characterization of the ferredoxin component of 25-hydroxycholecalciferol 1 alpha-hydroxylase. | the chick kidney mitochondrial iron--sulphur protein (ferredoxin), a component of the nadph--cytochrome p-450 reductase functional in the 1 alpha-hydroxylation of 25-hydroxycholecalciferol, was purified to homogeneity by chromatography on deae-cellulose, gel filtration on sephadex g-100 and preparative electrophoresis on polyacrylamide gel. a novel nadph--cytochrome c reductase assay utilizing crude renal nadph--ferredoxin reductase was used for the detection of the ferredoxin. a mol. wt. of 53 ... | 1979 | 219848 |
| prevention of spontaneous leukemia in akr mice by type-specific immunosuppression of endogenous ecotropic virogenes. | akr/j mice, 80-90% of which ordinarily die of spontaneous lymphocytic leukemias by 12 months of age, were significantly protected from developing leukemia in the initial experiment by a single course of treatment with akr serotype-specific antibodies mad in goats and processed as immune gamma globulin (igg). in experiment 1, igg was given on the day of birth and on four additional days, and finished on day 14. this schedule resulted in suppression of over 4 logarithms of normal virogene expressi ... | 1976 | 188047 |
| susceptibility and serological response of cattle, pigs, horses and goats to indiana2 and indiana3 strains of vesicular stomatitis virus. | | 1977 | 220637 |
| bluetongue antibody in botswana's domestic and game animals. | bluetongue precipitating antibody was demonstrated in sera of cattle, camels, sheep, goats and seven game species. of the domestic species the percentage of sera positive were; cattle 92%, camels 81%, goats 83% and sheep 36%. sheep sera, unlike those of other domestic species, varied greatly from area to area in the percentage positive. seroconversions were recorded in adult sheep between september and april. in adult cattle there was a gradual decline in the percentage positive with increasing ... | 1979 | 220761 |
| continued presence of similar transformation-associated antigens related to murine oncornavirus proteins in -ransformed cells, morphological revertants, and cells restricted in the expression of transformation. | the possible alteration of transformation-related antigens in cells that revert to a normal phenotype but that continue to retain the viral genome, has been investigated in [3h]glucosamine-labeled extracts of rat cells exhibiting a reversible temperature-dependent restriction in the expression of transformation and in a comparison of a morphologically altered mouse cell transformed by the kirsten sarcoma virus with a flat revertant mouse cell derived from the morphologically transformed cells. w ... | 1977 | 191180 |
| isolation and culture of endothelial cells from the lungs of small animals. | techniques are descirbed for the isolation and culture of endothelial cells from the lungs of small animals. the cells are collected by retrograde perfusion of blood-free lungs with buffered saline containing collagenase. the cells are characterized by light microscopy, electron microscopy of thin sections and surface replicas, and by the presence of angiotensin-converting enzyme (ace). ace was assayed using 3h-benzoyl-phe-ala-pro as substrate and was localized by indirect immunofluorescence usi ... | 1979 | 222181 |
| [light microscopic, electron microscopic and enzyme histochemical study of cerebral cortex of cattle, sheep and goat]. | | 1975 | 175114 |
| tumor-bound immunoglobulin in human gynecologic cancers. | by the use of immunofluorescence techniques, immunoglobulin of the igg class was consistently found in touch preparations and in frozen sections of squamous cell carcinomas of the uterine cervix (both keratinizing and nonkeratinizing) and in an adenosquamous cell carcinoma of the cervix, an adenocarcinoma of the cervix, a mixed mesodermal-uterine tumor, and a uterine adenocarcinoma metastasized to the ovaries. trace amountsof igm were found in 1 squamous cell carcinoma and in 1 adenocarcinoma of ... | 1979 | 222928 |
| demonstration of cytotoxic lymphocytes to virus-infected target cells in pigs inoculated with transmissible gastroenteritis virus. | lymphocytes, cytotoxic to virus-infected target cells, were induced in pigs orally exposed to transmissible gastroenteritis virus. they were studied and experiments were carried out by using autochthonous testicle cells as target cells to avoid genetic incompatibility of effector lymphocytes and target cells. cytotoxic lymphocytes were demonstrated in peyer's patches, mesenteric lymph nodes, spleen, and peripheral blood on postinoculation day (pid) 7. cytotoxic activity of lymphocytes increased ... | 1979 | 223476 |
| visna virus-induced fusion of nerve cells in vitro. | | 1979 | 224264 |
| testing rodents with virus infection associated antigen following a foot-and-mouth disease epizootic in india. | | 1979 | 224562 |
| a staphylococcal binding method for identification of antigens on murine leukemia virus infected cells. | | 1979 | 225387 |
| the occurrence of infectious diseases in mixed farming of domesticated wild herbivores and domestic herivores, including camels, in kenya. i. viral diseases: a serologic survey with special reference to foot-and-mouth disease. | | 1979 | 225576 |
| measurement of apolipoprotein a-i in rat high density lipoprotein and in rat plasma by radioimmunoassay. | a double antibody radioimmunoassay (ria) for rat apolipoprotein a-i is reported. the apoa-i isolated from delipidated hdl by gel filtration yielded a single band on polyacrylamide gel electrophoresis in sodium dodecyl sulfate (sds), and its amino acid composition resembled that reported by others. apoa-i was iodinated by lactoperoxidase and the resulting 125i-apoa-i was purified by gel filtration. up to 93% of 125i-apoa-i was precipitable by antibody and greater than 99% of bound 125i-apoa-i was ... | 1976 | 176289 |
| susceptibility of mink to certain viral animal diseases foreign to the united states. | mink (mustela vison) were inoculated with viruses: african horse sickness (ahs), african swine fever (asf), bovine herpes virus ii (bhv2), foot-and-mouth disease (fmd), goat pox (gp), hog cholera (hc), peste des petits ruminants (ppr), rinderpest (rp), swine vesicular disease (svd), vesicular exanthema of swine (ves) and vesicular stomatitis (vs). their susceptibility was measured by development of clinical signs, virus isolation and detection of precipitin and/or virus neutralizing antibodies. ... | 1979 | 228092 |
| pas-positive inclusions in the alveolar type ii cells of the caprine lung. | histochemical analysis of inclusions in the alveolar type ii cells was done with the alcian blue-pas technique. pas-positive inclusions, which were absent in the sheep and buffalo, were detected in the alveolar type ii cells of the goat lung. mixed diamine-sodium chloride did not stain these inclusions which also showed orthochromatic blue reaction with toluidine blue. the high percentage of alveolar type ii cells showing these inclusions suggested that this difference could be explained by the ... | 1979 | 228366 |
| the effect of dietary composition on the binding of insulin and glucagon to goat hepatocytes [proceedings]. | | 1979 | 229040 |
| incidence of bluetongue virus precipitating antibodies in sera of some domestic animals in the sudan. | to determine the presence and prevalence of bluetongue (bt) infection in a variety of domestic animal species in different geographical regions of the sudan, a serological study using the agar gel precipitation technique was initiated. a total of 2142 serum samples were examined. of the numbers tested approximately 28% of sheep, 11.2% of goats, 8% of cattle and 4.9% of camels were positive for group-specific antibodies to bt virus antigen, indicating previous exposure to bt infection. none of th ... | 1979 | 229163 |
| apolipoprotein a-ii content of human plasma high density lipoproteins measured by radioimmunoassay. | a double antibody radioimmunoassay for human apoa-ii is reported. apoa-ii isolated from human plasma high density lipoprotein (hdl) by column chromatography migrated as a single band on polyacrylamide disc gel electrophoresis, had the appropriate amino acid composition, and provoked the production of monospecific antisera. (125)i-apoa-ii (iodinated by lactoperoxidase, purified by sephadex g-75 chromatography) migrated with "cold" apoa-ii as a single band on disc gel electrophoresis in sds. its s ... | 1977 | 198505 |
| immunofluorescent localization of angiotensin converting enzyme in epithelioid and giant cells of sarcoidosis granulomas. | angiotensin converting enzyme (ace) (dipeptidyl carboxypeptidase; peptidyldipeptide hydrolase, ec 3.4.15.1) was localized in epithelioid and giant cells within ace-rich sarcoidosis granulomas, but not control granulomas, by immunofluorescence by using a rabbit anti-human ace immunoglobulin for localization and fluorescein-labeled goat anti-rabbit immunoglobulin for detection. ace was frequently not detected in the very central epithelioid cells, perhaps due to a paucity of cellular or molecular ... | 1979 | 230518 |
| endocrinology of lactation. | hormones are necessary for the growth of mammary glands, for initiation of the secretory process, and for the maintenance of an established lactation in all mammals. hormonal changes which occur in late pregnancy and the early postpartum period are described and graphed. this data was derived from research with animal models and relevant human data. enzymatic and cytologic differentiation of the mammary gland and limited mammary secretion begin during late pregnancy. this constitutes the 1st ... | 1979 | 230600 |
| experimental infection of goats with parainfluenza virus type 3. | | 1979 | 230666 |
| antibody to rotavirus in various animal species. | | 1979 | 231743 |
| [herpesvirus infection in kids in switzerland]. | | 1979 | 231824 |
| [cysticercus in animals slaughtered in the port-bouet abattoir (abidjan)]. | | 1978 | 232291 |
| chemically modified porous silica gel as a bioadsorbent and a biocatalyst. | | 1979 | 232385 |
| isolation of parapox viruses from man and animals: cultivation and cellular changes in bovine fetal spleen cells. | | 1979 | 232437 |
| analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus. | solid-phase enzyme immunoassays can be utilized to detect antigens directly in clinical specimens. however, a small number of stools which we tested for human rotavirus by enzyme-linked immunosorbent assay (elisa) were found to have nonspecific activity in the absence of rotaviral antigen. similar nonspecific activity was found in eight of eight sera which contained rheumatoid factor. this nonspecific activity was markedly reduced by pretreatment of the specimens with reducing agents, normal goa ... | 1979 | 232506 |
| isolation and identification of the sersenk strain of goat pox virus in iraq. | goat pox virus was isolated during an outbreak of pox infection among goats in the sersenk district, iraq. the isolated virus grew on the chorioallantoic membranes of developing chick embryos and in primary lamb testis cell cultures. it was identified morphologically as a pox virus and serologically as a member of the capripoxvirus group in the family poxviridae. the isolated virus was designated the "sersenk" strain. | 1979 | 233273 |
| suppression of the excitatory adrenergic neurotransmission; a possible role of cholinergic nerves in the retractor penis muscle. | the excitatory adrenergic response to transmural nerve stimulation of the isolated retractor penis (rp) of dog, cat, horse, boar, elk, bull, ram and goat, as well as the evoked release of 3h from dog rp preloaded with 3h-noradrenaline were studied. in untreated preparations a low concentration of scopolamine could markedly increase the excitatory adrenergic response. on the contrary, physostigmine per se suppressed this response and so did acetylcholine. scopolamine or atropine totally prevented ... | 1977 | 200067 |
| converting enzyme, angiotensin ii and hypertensive disease. | | 1978 | 203191 |
| volume, adherence properties, membrane antigens and mitogen responsiveness of rabbit lymphoid cell subpopulations. | subpopulations of rabbit spleen cells which respond to t and b mitogens, respectively, can be distinguished by sedimentation velocity in the earth's gravitational field. t cell subpopulations which differed in their responsiveness to con a and to pha could be identified by differences in adherence properties and by their sensitivity to complement mediated cell kill with rtla-antiserum. | 1975 | 234909 |
| central nervous system immunity in mice infected with theiler's virus. i. local neutralizing antibody response. | experimental theiler's mouse encephalomyelitis virus (tmev) infection in mice is atypical of most other picornavirus infections because virus persists in the host. it was shown previously that low levels of infectious virus are readily detectable in the central nervous system (cns) despite the presence of substantial titers of serum neutralizing antibody. in this study antibody assays were performed on cns tissue homogenates, and neutralizing antibody was regularly found in the cns of tmev-infec ... | 1978 | 203636 |
| preparation and characterization of an antiserum to cultured human oat-cell carcinoma cells. | viable cultured oat-cell carcinoma cells were used to immunize a goat. the resulting antiserum contained high titres of anti-normal activity and antibodies to cea. it was also shown, by indirect immunofluorescence, using fluorescein-conjugated rabbit anti-goat ig, to localize at high titres on the surface membranes of human lung cancer cells of 4 different histological types. booster immunizations produced a maximum secondary response one week after 2 weekly injections. the course of each immuni ... | 1978 | 207297 |
| serological evidence of the occurrence of bluetongue in iraq. | precipitating antibodies against bluetongue were detected in sheep and goat serum samples collected from animals slaughtered in baghdad abattoir. out of 294 sheep serum samples and 110 goat serum samples examined, 28 and 18 samples respectively showed precipitating activity. in addition, examination of sheep serum samples collected from localities where clinical cases similar to bluetongue were previously reported revealed the presence of bluetongue precipitating antibodies in 101 sera out of 19 ... | 1978 | 208202 |
| canine pulmonary angiotensin-converting enzyme. physicochemical, catalytic and immunological properties. | antiontensin-converting enzyme (peptidyldipeptide hydrolase, ec 3.4.15.1) has been solubilized from canine pulmonary particles and purified to apparent homogeneity. a value of approx. 140000 was estimated for the molecular weight of the native and the reduced, denatured forms of the enzyme. no free nh2-terminal residue was detected by the dansylation procedure. carbohydrate accounted for 17% of the weight of the enzyme, and the major residues were galactose, mannose and n-acetylglucosamine with ... | 1978 | 208622 |
| the effect of anemia on the ventilatory response to transient and steady-state hypoxia. | the effects of anemia upon the ventilatory responses to transient and steady-state hypoxia were studied in unanesthetized goats. responses to transient hypoxia (inhalation of several breaths of nitrogen) were considered to reflect peripheral chemoreceptor and non-chemoreceptor influences of hypoxia upon ventilatory control. in all goats, severe anemia (hemoglobin 3.1-4.8 g/100ml) markedly heightened the responses to transient hypoxia (from a mean of 0.27 to a mean of 0.75 liter/min/percent fall ... | 1975 | 236329 |