| effect of hydrophobic probes on the higher structure of d-amino acid oxidase. | 1. the holoenzyme of d-amino acid oxidase d-amino acid: o2 oxidoreductase (deaminating), ec 1.4.3.3 was found to combine with 1-anilinonaphthalene-8-sulfonate without liberation of its coenzyme, fad. no energy transfer interaction was found to occur between the bound dye and fad of the holoenzyme. on the other hand, when the apoenzyme was bound to the dye and then to fad, energy transfer interaction between the bound dye and bound fad was observed. in both cases, the dye competes with the substr ... | 1977 | 16661 |
| a study of the in vitro and in vivo interaction of barium sulphate particles with gastro-intestinal mucus. | the in vivo interaction of various radiopaques with mucus was investigated by a determination of the ph-mobility curves of the treated particles. it was found that mucus covered the radiopaque particles both in vivo and in vitro. this interaction was not affected by the nature of the radiopaque or by the state of the mucous membrane. the changed state of the mucus was modified by the diet of the rat from which it was derived. the results are discussed with reference to the technique used in the ... | 1976 | 16731 |
| the energy metabolism of the leukocyte. viii. the determination of the concentration of the coenzymes nad, nadh, nadp and nadph in polymorphonuclear leukocytes at rest and after incubation by enzymatic cycling. | | 1977 | 16746 |
| identification of an enzymatically active intermediate in the activation of porcine pepsinogen. | | 1977 | 16778 |
| mechanism of anti-inflammatory activity of substituted 4-quinazolone salicyl esters. | | 1977 | 16826 |
| ion pair formation in pig heart lipoamide dehydrogenase: rationalization of ph profiles for reactivity of oxidized enzyme with dihydrolipoamide and 2-electron-reduced enzyme with lipoamide and iodoacetamide. | | 1977 | 16887 |
| the nature of the interaction between ristocetin-willebrand factor viii coagulant activity molecule. | | 1977 | 16975 |
| effect of beta-adrenergic drugs on adenosine 3', 5'-monophosphate in rabbit vagus nerve. | | 1977 | 16990 |
| use of 1-anilino-8-naphthalene-sulfonate as a probe of gastric vesicle transport. | the interaction of 1-anilino-8-naphthalene-sulfonate (ans) with vesicles derived from hog fundic mucosa was studied in the presence of valinomycin and with the addition of atp. evidence was found for two classes of sites, those rapidly accessible to ans with a kd of 7.5 micronm and those slowly accessible, but rapidly accessed in the presence of valinomycin with a kd of 2.5 micronm. atp transiently increases the quantum yield of the latter ans binding sites only in the presence of valinomycin, b ... | 1977 | 17006 |
| cation transport by gastric h+:k+ atpase. | a vesicular microsomal fraction isolated from hog fundic mucosa demonstrates the capacity to take up equal amounts of rb+ and cl-. the amount of the rb+ uptake is sensitive to the extravesicular osmolarity, and rate of uptake is sensitive to temperature. 86rb+ efflux is dependent upon the cation composition of the diluting solution. atp, but not beta-gamma methylene atp, induces a reversible efflux of 86rb+ from loaded vesicles, and this is dependent upon a functional k+-atpase. the atp induced ... | 1977 | 17007 |
| a new antihypertensive compound: 3 hydrazino-6- (2-hydroxypropyl)methylamino pyridazine dihydrochloride (isf 2123). | | 1976 | 17122 |
| plasma secretin concentration in anaesthetized pigs after intraduodenal glucose, fat, aminoacids, or meals with various ph. | the concentration of immunoreactive secretin in portal blood and the secretion from the exocrine pancreas were measured during intraduodenal infusion of isotonic or hypertonic saline, isotonic or hypertonic glucose, aminoacids, fat emulsion, or 0.1 mol x 1(-1) hydrochloric acid in 7 anaesthetized pigs. none of these substances, except hydrochloric acid, had any effect on plasma secretin concentration and pancreatic flow rate and bicarbonate output. plasma secretin concentration rose significantl ... | 1977 | 17153 |
| a critical role for copper in aortic elastin structure and synthesis. | | 1977 | 17273 |
| in vitro study of beta-adrenergic receptors. | | 1977 | 17362 |
| pharmacologic evaluation of ci-775, a cardioselective beta adrenergic antagonist. | | 1977 | 17372 |
| inhibition of 4-aminobutyrate transaminase by ethanolamine-o-sulfate. | the analysis of the interaction of ethanolamine-o-sulphate with 4-aminobutyrate transaminase revealed that the inhibitory effect is exerted upon the substrate subsite of the active site of the enzyme in aldimine form. the inhibition in irreversible. the inactivation rate versus ph-curve was shown to have a sigmoid character with inclination point at neutral ph. the study of inhibition kinetics by the kitz and wilson method revealed a complex inhibitory pattern compatible with a minimal two-step ... | 1976 | 17433 |
| flow cytometric measurement of peptidases with use of 5-nitrosalicylaldehyde and 4-methoxy-beta-naphthylamine derivatives. | enzyme activity can be measured in single cells or in cell suspensions by either static or flow microfluorometry when a fluorogenic substrate is used. we have used amino acid derivatives of arylamines as fluorogenic substrates for tagging cellular proteinases. the liberated aromatic amine, which can diffuse from the cell, is trapped as a fluorescent insoluble schiff-base product with 5-nitrosalicylaldehyde, with the peak of fluorescence emission shifted from lambdaem 425 nm to lambdaem 530 and 5 ... | 1977 | 17486 |
| predictability of arterial ph and pco2 from right atrial blood samples in experimental animal. | arterial and right atrial blood samples were drawn simultaneously under acutely changing acid-base and ventilatory conditions in a series of experiments carried out in nine pigs. the ph, pco2, bicarbonate concentration and base excess from arterial and right atrial blood are compared. the predictability of the arterial values from the right atrial values is discussed. it is concluded that right atrial blood samples are approximately as informative as arterial blood samples for determining the ac ... | 1977 | 17513 |
| the metabolism of phenmetrazine in man and laboratory animals. | | 1977 | 17519 |
| a fluorometric method to assay chloramphenicol. | | 1977 | 17522 |
| pharmacokinetics of intravenously injected chloramphenicol in baby pigs. | a sensitive fluorometric assay procedure for chloramphenicol and its metabolites was used to measure distribution in tissues and elimination of the drug after injection of 22 mg/kg iv in baby pigs. disappearance of chloramphenicol from plasma was biphasic; an initial component was rapid (half-life about 9 min) and a second component had a half-life of 55 min. the total chloramphenicol levels in tissues and body fluids had half-life periods of 1-1.5 hr and dropped below limits of detectability wi ... | 1977 | 17523 |
| in vitro pharmacology of the opioid peptides, enkephalins and endorphins. | in the guinea-pig ileum methionine-enkephalin, normorphine and morphine are equipotent in depressing electrically evoked contractions; leucine-enkephalin has about 25% of the activity. the mouse vas deferens is more sensitive to the enkephalins which are 30 to 60 times more potent than morphine. fragments of beta-lipotropin61-91 (beta-endorphin) having sequences up to lph76 are more potent in the mouse vas deferens than in the guinea-pig ileum but beta-endorphin is about equipotent in the two pr ... | 1977 | 17538 |
| glutamic-oxalacetic transaminase activity in seminal plasma of some domestic animals in dependence of ph value and concentration of l-aspartate (author's transl). | | 1976 | 17556 |
| on the apparent inhibition of intramolecular activation of pepsinogen by pepsin substrates. | marciniszyn et al. (marciniszyn, j., huang, j. s. hartsuch, j. a., tang, j. (1976) j. biol. chem. 251, 7095-7102) have recently suggested an intermediate in the intramolecular activation of pepsinogen. as evidence, they showed apparent competitive inhibition of activation by globin, indication a pepsinogen-globin complex. previous work had shown pepsinogen activation to occur very rapidly in the presence of high concentrations of hemoglobin, a very similar pepsin substrate (mcphie, p. (1974) bio ... | 1977 | 17596 |
| the local anaesthetic activity of metiamide, a histamine h2- receptor antagonist. | | 1977 | 17678 |
| the effects of prostaglandins e1, e2, f1alpha and f2alpha on guinea-pig ileal and colonic peristalis. | prostaglandins (pge1, e2, f1alpha and f2alpha) have been tested on the peristaltic reflex in isolated segments of guinea-pig ileum and colon using simultaneous recordings of fluid propulsion and longitudinal and circular muscle activity. propulsion and circular muscle peristaltic activity were increased by serosally applied pgf compounds in the ileum and pge or pgf compounds in the colon following initial contraction of the longitudinal muscle. this is consistent with a role for prostaglandins i ... | 1977 | 17690 |
| haloperidol inhibits contractions of the vas deferens. | | 1977 | 17701 |
| further observations on an ether-o-oxidase, formerly called alkyl etherase, from liver tissue. | 1. the microsomal enzyme from liver previously called an "etherase" is now described more accurately as an ether-o-oxidase. it has been investigated further to free it from the membranes in aqueous solution and to try to define its physiological substrate. 2. after a variety of attempts with detergents, etc., the enzyme was obtained in impure solution from precipitation with 35-45% (nh4)2so4 solution after a short digestion at room temperature. 3. when a suitably reinforced the enzyme in solutio ... | 1977 | 17745 |
| a comparison of the antiserotonin, antihistamine, and anticholinergic activity of cyproheptadine with analogues having furan nuclei fused to the 10,11-vinylene bridge. | a series of cyproheptadine derivatives having furan nuclei fused to the 10,11-vinylene bridge has been prepared. none of the compounds retain the potent antiserotonin and antihistaminic actions of cyproheptadine. 1-methyl-4-(1-methyl-8h-dibenzoa,efuro3,4-ccyclohepten-8-ylidene)piperidine (7), 1-methyl-4-(1,3-dihydro-1-oxo-8h-3,4:6,7cycloheptal1,2-cfuran-8-ylidene)piperidine (10), and its reduction product 11 retained the peripheral anticholinergic activity of cyproheptadine. | 1977 | 17748 |
| beta-adrenoceptor blocking activity of halogenated thienylethanolamine derivatives. | the synthesis of a number of ring-halogenated n-isopropyl- and n-tert-butyl-2-amino-1-(2-thienyl)ethanols has been carried out in order to ascertain the influence of chloro or bromo substitution at the thiophene moiety on their blocking beta-adrenoceptor activity. it was found that chloro- and bromo-substituted compounds exhibited a similar activity. monohalo substitution at positions c4 or c5 of the thiophene ring resulted in comparable blocking potency, whereas c3 halo-substituted compounds we ... | 1977 | 17752 |
| identification of essential histidine residues of aminoacylase by photooxidation and by reaction with diethylpyrocarbonate. | state and function of the histidine residues of aminoacylase were investigated by photoxidation in the presence of methylene blue and by chemical modification with diethylpyrocarbonate. complete inactivation of the enzyme was observed after oxidation of 4 histidine residues. from the ph dependence of the photooxidation it becomes evident that the inactivation of the enzyme is not a consequence of the simultaneous oxidation of tryptophan residues. the enzyme is also inactivated by chemical modifi ... | 1977 | 17959 |
| properties of the laryngeal chemoreflex in neonatal piglets. | cardiorespiratory reflex responses to laryngeal chemoreceptor stimulation were studied in 62 piglets of both sexes varying in age from 1 to 79 days. the distal trachea was cannulated to provide a free airway and the proximal end used to introduce fluids into the laryngeal area. introduction of either water or milk produced apnea, bradycardia, and hypertension. swab application of test fluids to the laryngeal epithelium produced similar responses. the reflex could be interrupted by flushing the l ... | 1977 | 18025 |
| new techniques to measure blood cholinesterase activity in domesticated animals. | a macromethod and a semimicromethod were developed to measure erythrocyte acetylcholinesterase activity in cattle, sheep, goats, horses, dogs, and swine, and to measure plasma cholinesterase activity in horses, dogs, and swine. comparison of the 2 methods with erythrocytes of sheep, cattle, goats, and horses indicated both methods gave similar results. they can be done in a shorter time and are more sensitive than michel's method. normal deltaph values per minutes, with standard deviations for b ... | 1977 | 18075 |
| isoelectric focusing of hog cholera virus. | the isoelectric point of hog cholera virus (alfort strain) has been determined (phi = 4.8). a clear stimulation of its infectivity can be recorded at this ph. the isoelectrophoretic profiles of strains isolated in the field show no significant differences. | 1977 | 18081 |
| significance of fecal coliform-positive klebsiella. | a total of 191 klebsiella pneumoniae isolates of human clinical, bovine mastitis, and a wide variety of environmental sources were tested for fecal coliform (fc) response with the membrane filtration and most probable number techniques. twenty-seven escherichia coli cultures of human clinical and environmental origins were also tested. eighty-five percent (49/58) of known pathogenic k. pneumoniae were fc positive, compared with 16% (19/120) of the environmental strains. e. coli results indicated ... | 1977 | 18086 |
| malate dehydrogenase: a higher molecular weight form produced by freeze-thaw treatment of pig heart supernatant enzyme. | | 1977 | 18109 |
| solvent interactions with the active site of the pig heart cytosolic aspartate aminotransferase. | | 1977 | 18112 |
| some aspects of the trachea-heart selectivity of rimiterol in vitro and in vivo in guinea-pigs. | | 1977 | 18120 |
| purification and properties of a new testosterone 17beta-dehydrogenase (nadp+) from guinea-pig liver. | as a result of studies of guinea-pig live testosterone 17beta-dehydrogenase (nadp+) (ec 1.1.1.64), a new testosterone 17beta-dehydrogenase was discovered. the new enzyme was purified to a single homogeneous protein from the 105 000 g-supernatant fraction of guinea-pig liver by (nh4)2so4 fractional precipitation and two gel-filtration stages, deae-cellulose column chromatography and hydroxyapatite column chromatography. it was characterized by many properties. the enzyme has almost the same prope ... | 1977 | 18133 |
| correlation of biologic data with physico-chemical properties among the vinca alkaloids and their congeners. | | 1977 | 18155 |
| inhibition of gastric k+ atpase by phenylbutazone and indomethacin. | | 1977 | 18156 |
| comparative pharmacological studies on beta-adrenolytic agents. an analysis of quantitative activity (author's transl). | | 1977 | 18159 |
| on the determination of isozyme levels in preparations containing cytoplasmic and mitochondrial aspartate aminotransferase. | a spectrophotometric assay has been developed for the determination of the content of each isozyme of aspartate transaminase (l-aspartate:2-oxoglutarate aminotransferase, ec 2.6.1.1) in physiological fluids or tissue extracts. the methods relies on the ability of adipate, at low ph and ionic strength to inhibit the cytoplasmic isozyme but not the one from mitochondria. two assays are necessary, one at ph 8.0 which measures the content of both isozymes and another at low ph which measures primari ... | 1977 | 18183 |
| reversible modification of amino groups in aspartate aminotransferase. | amino groups in the pyridoxal phosphate, pyridoxamine phosphate, and apo forms of pig heart cytoplasmic aspartate aminotransferase (l-aspartate: 2-oxoglutarate aminotransferase, ec .2.6.1.1) have been reversibly modified with 2,4-pentanedione. the rate of modification has been measured spectrophotometrically by observing the formation of the enamine produced and this rate has been compared with the rate of loss of catalytic activity for all three forms of the enzyme. of the 21 amino groups per ... | 1977 | 18199 |
| partial purification and characterization of a triacyglycerol lipase from rat liver cytosol. | a triacyglycerol lipase (ec 3.1.1.3) was purifiec about 60-fold from rat liver cytosol by delipidation with acetone and ethyl ether, hydroxyapatitie and sephadex g-100 column chromatographies and isoelectrofocusing electrophoresis. the partially purified enzyme had a molecular weight of approximately 42 000 and an isolectric point of 7.2. the km for trioleylglycerol was 0.33 mm and the ph optimum was around 8.0. the activity of the enzyme was not dependent on serum lipoproteins, but was stimulat ... | 1977 | 18201 |
| immobilization and characterization of hog kidney mutarotase. | | 1977 | 18232 |
| the use of functional antagonism to determine whether beta-adrenoceptor agonists must have a lower efficacy than isoprenaline to be trachea-atria selective in vitro in guinea-pigs. | 1 the relative efficacies of three trachea-atria selective beta-adrenoceptor agonists, fenoterol, me 506 and me 454, compared to isoprenaline, were determined on both trachea and atria of guinea-pigs. 2 on tracheal preparations the beta-adrenoceptor agonists were used as functional antagonists of carbachol and a comparison of the maximum shifts in the carbachol concentration-response line produced by each of the beta-adrenoceptor agonists provided a comparison of their efficacies. 3 on atrial pr ... | 1977 | 18249 |
| interactions of citrate synthases from osmoconforming and osmoregulating animals with salt: possible signs of molecular eco-adaptation? | this study considers differential sensitivity of citrate synthase (citrate oxaloacetatelyase coa acetylating) ec 4.1.3.7. from an osmoconforming animal (sea anemone) and an osmoregulating animal (the pig) to salt. attention is drawn to the fact that the osmoconforming sea anemone is in essence a sessile creature while the pig is readily mobile and able to change its ionic environment at will. it had been shown earlier that citrate synthase from another osmoconformer (oyster) is also not sensitiv ... | 1977 | 18342 |
| inter-relationships between proton electrochemical gradient, adenine-nucleotide phosphorylation potential and respiration, during substrate-level and oxidative phosphorylation by mitochondria from brown adipose tissue of cold-adapted guinea-pigs. | | 1977 | 18347 |
| pig plasma benzylamine oxidase: some considerations on the mechanism of the reaction. | the kinetics of benzaldehyde formation during the oxidation of benzylamine by pig plasma benzylamine oxidase have been studied at different ph values. it has been shown that the first step of the reaction catalyzed by this enzyme is the formation of a schiff base between the amino group of the substrate and the aldehyde group of the enzyme. present kinetic studies are consistent with this mechanism and demonstrate the existence of two steps which are alternatively rate-limiting one below ph 6.0, ... | 1977 | 18426 |
| induction of a pale, soft, exudative-like myopathy and sudden death in pigs by injection of anterior pituitary extract. | | 1977 | 18430 |
| effect of dietary protein during growth and gestation on development and reproductive performance of gilts. | | 1977 | 18432 |
| preparation of myosin subfragment-1 from pig cardiac muscle myosin by chymotryptic digestion. | | 1977 | 18456 |
| aspartate aminotransferase. determination of the active site occupancy pattern indicates independent transamination of the two subunits. | | 1977 | 18474 |
| evidence that acetylcholine releases noradrenaline in the sympathetic fibre. | | 1977 | 18567 |
| effects of sympathetic nerve stimulation on electrical activity of auerbach's plexus and intestinal smooth muscle tone. | | 1977 | 18581 |
| bradykinin relaxes contracted airways through prostaglandin production. | | 1977 | 18587 |
| nature of the rabbit acrosome reaction-inducing activity of follicular fluid. | follicular fluid samples from rabbits, cats, pigs, women and cows had acrosome reaction-inducing activity (aria) on rabbit spermatozoa as determined by differential staining after incubation with these fluids. activity was retained after dialysis and at least 50% was found to be labile when heated to 56 degrees c for as little as 20 min. the induction of the acrosome reaction by bovine follicular fluid showed a dependence on the concentration of follicular fluid and spermatozoa and on calcium io ... | 1977 | 18603 |
| analysis of hypotensive mechanisms of pindolol, a beta-adrenoceptor blocking drugs in rats. | the hypotensive mechanisms of pindolol in anesthetized and conscious rats were investigated. pindolol caused a fall in blood pressure in anesthetized, conscious and spinal rats, though in conscious rats a higher dose of the drug was required to produce such a response. this hypotension with pindolol in anesthetized and conscious rats was markedly inhibited by pretreatment with the drug itself or other beta-adrenoceptor blocking drugs. a similar phenomenon also occurred when isoproterenol was inj ... | 1977 | 18631 |
| calcium ions and relaxation of intestinal smooth muscle induced by papaverine and aspaminol. | | 1977 | 18632 |
| effects of calcium-removal and sodium-potassium adenosine triphosphatase inhibition on the release of epinephrine from the extraneuronal site. | | 1977 | 18643 |
| beta-adrenoceptor studies. 4. influence of albumin on in vitro beta-adrenoceptor blocking and antiarrhythmic properties of propranolol, pindolol, practolol and metoprolol. | | 1977 | 18685 |
| studies on tissue culture produced plasminogen activator. i. preliminary observations and the enhancing effect of colchicine and other antimitotic agents. | | 1977 | 18812 |
| replication of swine and equine influenza viruses in canine kidney cells. | both swine and equine influenza viruses were replicated in madin-darby canine kidney cells. hemagglutinin and complement-fixing antigens were synthesized at 4 to 8 hours postinfection and reached maximal synthesis at 16 to 20 hours. neuraminidase activity increased 9- to 11-fold; 50% of the total hemagglutinin antigen was made before the infective virus progeny was detected. | 1977 | 18972 |
| biochemical characterization of histamine-sensitive adenylate cyclase in mammalian brain. | | 1977 | 18994 |
| alpha-d-fucosidase activity of human and pig kidney: a property of alpha-d-galactosidase. | | 1977 | 18995 |
| redox properties of microsomal reduced nicotinamide adenine dinucleotide-cytochrome b5 reductase and cytochrome b5. | hepatic nadh-cytochrome b5 reductase was reduced by 1 mol of dithionite or nadh per mol of enzyme-bound fad, without forming a stable semiquinone or intermediate during the titrations. however, the addition of nad+ to the partially reduced enzyme or illumination in the presence of both nad+ and edta yielded a new intermediate. the intermediate had an absorption band at 375 nm and the optical spectrum resembled anionic semiquinones seen on reduction of other flavin enzymes. electron paramagnetic ... | 1977 | 19038 |
| structure-function relationships in tpn-dependent isocitrate dehydrogenase. i. electron paramagnetic resonance studies of the interaction of enzyme-bound mn(ii) with substrates, cofactors, and substrate analogues. | electron paramagnetic resonance (epr) spectra were obtained for various isocitrate dehydrogenase-mn(ii) complexes. the qualitative effects of the binding of substrates, nucleotides, and substrate analogues on the isotropic character of the electronic environment of enzyme-bound mn(ii) were subsequently investigated. the addition of isocitrate produces a markedly anisotropic spectrum whereas alpha-ketoglutarate does not alter the spectrum of enzyme-mn(ii) substantially. this suggests direct coord ... | 1977 | 19044 |
| structure-function relationships in tpn-dependent isocitrate dehydrogenase. ii. determination of the paramagnetic relaxation rates of water protons in complexes of enzyme, mn(ii), substrates, cofactors, and inhibitors. | | 1977 | 19045 |
| effect of ph on the interaction of benzoate and d-amino acid oxidase. | the kinetic and equilibrium dissociation constants of the reversible binding of benzoate to hog kidney d-amino acid oxidase (daao) were studied at 19 degrees c over the ph range 5.3-10.5 by means of a stopped-flow apparatus and spectrophotometric titrations. a simple bimolecular reaction of the form second order-first order was observed; a two-step reaction was seen. analysis of the ph dependence of the bimolecular rate constants and equilibrium dissociation constants is consistent with three io ... | 1977 | 19047 |
| the role of arginine in the triphosphopyridine nucleotide dependent isocitrate dehydrogenase of pig heart. | | 1977 | 19048 |
| mechanism of refolding and reactivation of lactic dehydrogenase from pig heart after dissociation in various solvent media. | | 1977 | 19049 |
| cyclic amp-dependent protein kinase phosphorylation of cardiac (na+ + k+)-atpases. effect on calcium binding. | 1. calcium binding to (na+ + k+)-atpase (atp phosphohydrolase, ec 3.6.1.3) preparations from beef and pig heart preparations of varying degrees of purity was measured. 2. binding was inhibited by mg2+, na+ and k+. inhibition by na+ and k+ appeared to be due to an ionic strength effect. 3. four classes of binding sites were identified with kd values for calcium of about 0.03, 1, 15 and 200 micrometer. 4. cyclic amp-dependent phosphorylation of the enzyme by protein kinase (atp: protamine o-phosph ... | 1977 | 19066 |
| enhanced heat, alkaline and tryptic stability of acetamidinated pig heart lactate dehydrogenase. | modification of 17 from 24 lysine residues in pig heart lactate dehydrogenase (l-lactate: nad+ oxidoreductase, ec 1.1.1.27) with methyl aceimidate yields an enzyme derivative with enhanced stability toward meat and alkaline denaturation as well as tryptic digestion. the specific activity of the modified enzyme is only slightly reduced | 1977 | 19075 |
| reconstitution and lipid requirements of porcine tissue thromboplastin. | | 1977 | 19079 |
| a potent new beta2-adrenoceptor blocking agent. | 1 (t-butyl-amino-3-ol-2-propyl) oximino-9 fluorene is a new beta2-adrenoceptor blocking agent with a pa2 of 9.23+/-0.25 on isolated trachea. 2 it provokes hypertension in normotensive rats and does not prevent arterial hypertension in shr rats, although it does prevent the renin secretion normally induced by isoprenaline infusion. | 1977 | 19116 |
| new beta-adrenoreceptor stimulants. studies on 3-acylamino-4-hydroxy-alpha-(n-substituted aminomethyl)benzyl alcohols. | | 1977 | 19155 |
| mechanism of action of antihypertensive agents. | | 1977 | 19198 |
| suppression of reactions to certain cosmetics. | reactions to hair dyes and bleaches may be "suppressed" with corticosteroids and antihistamines. reactions to nail polish may be prevented by a "drying" or "polymerizing" technique. sensitization to certain perfume ingredients may be inhibited by a "quenching" phenomenon. | 1977 | 19206 |
| immobilised lipoamide dehydrogenase. 1. preparation and properties of the enzyme immobilised by disulphide interchange. | | 1977 | 19245 |
| the effective proton conductance of the inner membrane of mitochondria from brown adipose tissue. dependency on proton electrochemical potential gradient. | the nucleotide-sensitive h+ (oh-) conducting pathway of mitochondria from the brown-adipose tissue of cold-adapted guinea-pigs passes an effective proton current which is directly proportional to the proton electrochemical gradient. at 23 degrees c and ph 7.0 this conductance is 16 nmol h+ - min-1 - mg-1 - mv-1. addition of 0.2 mm gdp results in a conductance which is linear and low (0.7 nmol h+ - min-1 - mg-1 - mv-1) until deltamicronh+ exceeds 220 mv. at higher values of deltamicronh+, which c ... | 1977 | 19250 |
| acetylcholine-norepinephrine interactions on potassium movements in the sinus node. | possible adrenergic mechanisms involved in acetylcholine (ach) induced potassium movements in the sinus node have been investigated using a tracer potassium (42k) and a microelectrode technique. the ach-induced increase in 42k uptake was enhanced by propranolol and was unaffected by phentolamine. reserpinization neither prevented the ach-induced increase in 42k uptake nor the enhanced effect in the presence of propranolol. in reserpinized preparations, ach-induced 42k uptake was the same before ... | 1977 | 19269 |
| modification of the action of pentagastrin on acid secretion by botulinum toxin. | i.v botulinum toxin after 60-90 min abolished the dose-response relationship between pentagastrin and gastric acid secretion in anesthetized rats and guinea-pigs. the toxin reduced but did not abolish the acid stimulatory effect of histamine. as expected, the acid response to vagal stimulation was abolished and that to methacholine in rats was unaltered by the toxin. | 1977 | 19277 |
| effects of alpha- and beta-adrenergic blockers on the actions of noradrenaline on body temperature in the newborn guinea-pig. | the effect of na injected into the lateral cerebral ventricle on tc was blocked by alpha-adrenergic receptor blockers, but not by beta-receptor blockers, whereas the effect of systemically administered na was blocked by i.p. administered beta-receptor blockers, but not by alpha-blockers. | 1977 | 19282 |
| regulation of phospholipase a2 activity by different lipid-water interfaces. | | 1977 | 19284 |
| stoicheiometries of proton translocation by mitochondria. | | 1977 | 19316 |
| glutamine synthetase from pig brain: binding of adenosine triphosphate. | glutamine synthetase was purified to homogeneity from fresh brain homogenates by a procedure that makes use of the affinity of this atp requiring ligase to cibacron 3g-a blue-sephadex g-75. it is shown that the interaction of pig brain enzyme with the dye does not concern the active centre but a non-catalytic although specific atp binding site. both sides contain a cysteine residue which may react with n-ethylmaleimide. the unprotected succinimidated enzyme is inactive, and aggregates. atp alone ... | 1977 | 19351 |
| effect of halide anions on the binding of fad to d-amino acid oxidase and the tryptophanyl fluorescence of the apoenzyme. | the quenching of tryptophanyl fluorescence of native and denatured d-amino acid oxidase from hog kidney was measured. about 60% of the tryptophanyl fluorescence of the native apoenzyme was quenched by iodide at ph 8.3, and 25 degrees c. all of the tryptophanyl fluorescence of the apoenzyme in 6 m guanidine hydrochloride was quenched. the tryptophanyl fluorescence quenching of the holoenzyme by 1-methyl nicotinamide chloride was low in comparison with that of the apoenzyme. these results of the q ... | 1977 | 19435 |
| a study of the interactions between flavoprotein and quasi-substrates. circular dichroism spectra of d-amino acid oxidase complexes. | | 1977 | 19436 |
| purification and properties of pigeon breast muscle alpha-keto acid dehydrogenase complexes. | | 1977 | 19448 |
| investigation of the relation of the ph-dependent dissociation of malate dehydrogenase to modification of the enzyme by n-ethylmaleimide. | the ph-dependent dissociation of porcine heart mitochondrial malate dehydrogenase (l-malate:nad+ oxidoreductase, ec 1.1.1.37) has been further characterized using the technique of sedimentation velocity ultracentrifugation. the increased rate and specificity of the inactivation of mitochondrial malate dehydrogenase by the sulfhydryl reagent n-ethylmaleimide has been correlated with the ph-dependent dissociation of the enzyme. data obtained using nad+ and its component parts to reassociate the en ... | 1977 | 19462 |
| n-acetyl-aspartate amidohydrolase: purification and properties. | | 1977 | 19559 |
| metabolic oxidation of aralkyl oximes to nitro compounds by fortified 9000g liver supernatants from various species. | incubation of 'amphetamine oxime' (iia, anti-benzyl methyl ketoxime) with fortified rabbit liver 9000 g supernatants gave the nitro compound (ie) and the beta-hydroxylated oxime (iic) in addition to the previously reported ketone (iib) and alcohol (ic) metabolites. formation of the products was cofactor dependent. the nitro compound was also formed using mouse, hamster and guinea-pig 9000 g liver supernatants and to a minor extent by rat liver. the oximes of 2-phenethylamine (iie) and norfenflur ... | 1977 | 19580 |
| the effects of diethylcarbamazine citrate on smooth muscle. | the effect of the anthelmintic drug diethylcarbamazine citrate (decc) was examined on the guinea-pig isolated ileum, rabbit duodenum, chick oesophagus, rat portal vein and pig coronary artery. decc contracted all the gastrointestinal smooth muscle preparations. the contractions were antagonized by hexamethonium and atropine but they were not affected by mepyramine or methysergide in concentrations that abolished, or markedly reduced, responses to histamine and 5-hydroxytryptamine. decc inhibited ... | 1977 | 19582 |
| enhancement by physalaemin of the contractions induced by cholinomimetics in the guinea-pig ileum. | | 1977 | 19590 |
| the extraction and analysis of benzodiazepines in tissues by enzymic digestion and high-performance liquid chromatography. | a simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin carlsberg followed by reverse-phase liquid chromatography. the enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatogrpahic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate "clean ... | 1977 | 19593 |
| an in vivo assay for the analysis of the biological potency and structure-activity relationships of narcotics: serum testosterone depletion in the male rat. | an in vivo method for the assessment of the agonistic-antagonistic activities and structure-activity relationships of the narcotics is described. the method, which is based on the depletion of serum testosterone levels by the narcotics in the male, satisfies all of the criteria necessary for a valid narcotic assay: 1) the ability of the narcotics to deplete serum testosterone levels is stereospecific; 2) naloxone competitively inhibits their effects; 3) and the relative potencies of the drugs in ... | 1977 | 19620 |
| pharmacologically active substances from the sea, ii. various cardiovascular activities found in the extracts of marine organisms. | | 1977 | 19670 |
| role of alteration of the mucosa of the gastrointestinal tract in the mechanism of disruption of mediator metabolism in allergic and dysenteric colitis. | | 1977 | 19723 |
| the alpha-action of catecholamines on the guinea-pig taenia coli in k-free and na-free solution and in the presence of ouabain. | | 1977 | 19746 |