| con a-inducible suppression of mlc: evidence for mediation by the th2 + t cell subset in man. | human peripheral lymphoid cells pretreated with concanavalin a for 48 hr can markedly suppress the proliferative response of untreated autologous lymphoid cells in mlc. isolation studies with sephadex g-200 anti-f(ab')2 affinity chromatography, nylon adherence, and e rosetting indicate that the con a-induced suppressor cell is a t cell. further fractionation into th2+ and th2- cell subsets with an equine-anti th2 serum show that both subsets can be activated by con a to an equivalent degree. aft ... | 1979 | 156215 |
| [pms and the ways to improve its quality]. | | 1979 | 156454 |
| the influence of spermine, spermidine and various sera on t-lymphocyte and granulocyte colony growth in vitro. | using t-lymphocyte (t-lc) and granulocyte colony (gc) assays with truly proliferating cells, the dose-response relationships of spermine and spermidine, fetal calf (fcs), calf (cs), horse (hs) and human ab serum (abs), and of the polyamines in the presence of selected does of the sera were analyzed. in contrast to earlier observations using [3h]thymidine uptake masurements it was found that the polyamines as well as the sera themselves inhibit t-lc and gc growth in the presence of autologous hs ... | 1979 | 157005 |
| [on the influence of thiocyanates on the delayed immune response in intact and epinephrectomised guinea pigs (author's transl)]. | daily application of 32 mg/kg sodium thiocynate from 1--11th respectively 12th--21st experimental day causes in epinephrectomised guinea pigs at the 26th experimental day an inhibition of cell-mediated immune response (sensitization was induced by intraperitoneal injection of horse serum at the 5th and 7th experimental day). the inhibition of cell-mediated immune response could be demonstrated by means of migration inhibition of spleen cells. epinephrectomy was performed by operation procedures ... | 1979 | 157682 |
| [outbreak of equine influenza in chile]. | | 1979 | 157748 |
| an outbreak of eosinophilic bronchitis in horses possibly associated with dictyocaulus arnfieldi infection. | eight mature horses which had been affected with a moist cough for six weeks were found to have large numbers of eosinophils in tracheal mucus samples taken by transtracheal washing. these horses were kept on irrigated pasture and fed a hay-free diet. a companion yearling donkey was found to be passing dictyocaulus arnfieldi larvae in its faeces. two oral treatments with a dose of thiabendazole (440 mg/kg) resulted in the resolution of the clinical signs and the disappearance of eosinophils from ... | 1979 | 157874 |
| the fourth sir frederick hobday memorial lecture. back problems in the horse--a look at past, present and future progress. | | 1979 | 158523 |
| veterinary experiences as a japanese prisoner of war and ex-pow along the burma railroad from 1942 to january 1946. | as a prisoner of war the writer was working for nearly three years in different pow camps, and outside them, along the burma railway from thanbyuyzat in southern burma up to kanchanabury in thiland. in the army of the netherlands-indian archipelago (knil) he had the military rank of reserve horse-doctor. in civilian life he was attached to the veterinary institute in buitenzorg(now bogor) as a veterinary bacteriologist. his task as a pow became that of meathygienist and supervisor of the living ... | 1979 | 158850 |
| development of the equine venous sinuses of the dura mater. | | 1979 | 158999 |
| [equine piroplasmosis and nuttaliosis]. | | 1979 | 159539 |
| amputation of the equine urethral process affected with habronemiasis. | | 1979 | 161109 |
| [purification and comparison of quail and chicken pepsinogens]. | pepsinogens of quail and chick, specific to adult proventriculus, were purified and their properties were compared. these two pepsinogens are similar in regard to enzymological characters, amino acid compositions, and immunological characters. | 1979 | 161196 |
| the growth of the transverse sectional area (tsa) of m. semitendinosus in the dog and horse and its relation to athletic ability in the two species. | | 1979 | 161486 |
| an investigation of seven enzymes as possible genetic markers in horse leucocytes. | in this paper we describe seven enzymes, np, gotm, pgm2, alpha fuc, pep a, ada and mpi which are found in the white cells of horses, including 39 british crossbred ponies and 16 crossbred horses, 30 mongolian ponies and 10 icelandic ponies. two of these enzymes--alpha fuc and mpi--were polymorphic in all the populations of horses studied and could prove useful as additional markers in the paternity testing of horses. pep a and gotm were also polymorphic in two of the populations studied and coul ... | 1979 | 161952 |
| characteristics of rat carcinoma in culture. | rat chloroma cells have been propagated in permanent suspension cultures and are grown in dulbecco's modified eagle's medium with 10% fetal calf serum and 2.5% horse serum. several lines have been established; the one of longest duration, mia c51, has been maintained for over 18 months and has undergone over 100 transfers. mia c51 cells have a doubling time of 12 hr and maintain many of the properties of the parent tumor, including the characteristic greenish color with high myeloperoxidase acti ... | 1975 | 162871 |
| hypogammaglobulinemia predisposing to infection in foals. | measurement of serum immunoglobulins in 46 foals less than 2 weeks old revealed 9 foals with hypogammaglobulinemia. the hypogammaglobulinemia was attributed to failure in transfer of immunoglobulins from dam to foal via colostrum. three of the affected foals did not nurse at all, or only slightly, and 2 of these died of infections within a few days after birth, whereas the 3rd foal did not grow as well as normal foals. six of the affected foals nursed in an apparently normal manner, and 5 of the ... | 1975 | 162901 |
| viral respiratory infections of horses: structure and function of lungs in relation to viral infection. | since the advent of cell culture techniques, numerous viruses have been shown to be related to respiratory diseases in horses. although the viruses differ in many ways, they cause disease with some common characteristics. this report is a summary of some of the available material from written sources and from personal observations. it is intended to help explain some of the changes observed in viral-induced respiratory disease. | 1975 | 162902 |
| viral respiratory infections of horses: some specific viruses affecting the horse. | | 1975 | 162903 |
| adenoviral infection in foals. | | 1975 | 162904 |
| clinical aspects of viral respiratory infections of horses. | | 1975 | 162905 |
| phosphorylation of lymphocyte nuclear acidic proteins: regulation by cyclic nucleotides. | guanosine 3',5'-monophosphate (cyclic gmp) and cholinergic agents stimulate incorporation of phosphate into specific nuclear acidic proteins of horse peripheral blood lymphocytes. agents that raise intracellular adenosine 3',5'-monophosphate (cyclic amp) inhibit nuclear acidic protein phosphorylation. the opposing effects of cyclic gmp and cyclic amp upon nuclear protein phosphorylation parallel the effects of the cyclic nucleotides upon induction of lymphocyte proliferation. | 1975 | 163491 |
| equine infectious respiratory disease. | during the past 20 years the equine population of great britain and ireland has increased with the result that the practising veterinary surgeon is more frequently called upon to advise on equine problems. a significant portion of this advice is concerned with the examination of horses showing signs of this advice is concerned with the examination of horses showing signs of respiratory disease. numerous pathogens, which include viruses, bacteria, parasites and moulds invade the respiratory tract ... | 1975 | 163516 |
| inhibition by iodide of the activation of the thyroid cyclic 3',5'-amp system. | the action of iodide on the cyclic amp system of dog thyroid slices has been studied. iodide inhibits the enhancement of cyclic amp accumulation in the presence of tsh. such an effect is also observed in horse, beef and sheep thyroid slices, but not in dog kidney slices stimulated by parathyroid hormone or in rat parotid slices stimulated by isoproterenol. the effect in dog thyroid slices is suppressed by 1mm naclo4, 1mm methimazole and 1mm propylthiouracil. similar data have been obtained for p ... | 1975 | 163738 |
| peroxidase activity of horse liver alcohol dehydrogenase in the presence of beta-nad+ and hydrogen peroxide. | | 1975 | 163766 |
| response of irradiated mice to live-virus (tc-83) immunization. | studies were conducted to determine the effects of sublethal, acute, total-body irradiation (600 r) on the immune response of mice to a replicating antigen. irradiation was performed at varying times (0 to 21 days) prior to immunization with attenuated venezuelan equine encephalomyelitis virus, tc-83. development of protective immunity was studied by inoculating subgroups of irradiated mice with virulent venezuelan equine encephalomyelitis virus on days 1 through 28 after immunization. irradiati ... | 1975 | 163795 |
| coenzyme interaction with horse liver alcohol dehydrogenase. evidence for allosteric coenzyme binding sites from thermodynamic equilibrium studies. | the techniques of fluorescence enhancement, fluorescence quenching, fluorescence polarization, and equilibrium dialysis are utilized to study the binding properties of coenzyme to horse liver alcohol dehydrogenase. polarization of fluorescence and equilibrium dialysis show that nadh binds to alcohol dehydrogenase with a stoichiometry of 6 mol per mol of enzyme, in contrast to the value of 2 determined from fluorescence enhancement measurements. nad+ also binds with a stoichiometry of six as was ... | 1975 | 163820 |
| spontaneous and induced mutagenesis in western equine encephalomyelitis virus in chick embryo cells with different repair activity. | evidence was obtained indicating differences in the survival rate of western equine encephalomyelitis virus after exposure to ultraviolet radiation and methyl methanesulfonate in commercial and leukosisfree chick embryo cells that differed in repair activity. the levels of spontaneous mutagenesis (on the basis of the yield of small palque variants of the encephalomyelitis virus) did not essentially change when the virus was passage in leukosis-free chick embryo cells, whereas an increase in the ... | 1975 | 164025 |
| growth cycle of arboviruses in vertebrate and arthropod cells. | | 1975 | 164052 |
| viral infection and interferon in cell cultures aged in vitro. | cells from leucosis-free chick embryos (skin and muscles), grown in vitro 1 and 7 days with no medium change, are used as a model for young dividing and stationary in vitro aged cells, respectively. aged cultures are advantageous for if induction by cytopathic and noncytopathic viruses. decreased virus production in them is ascribed to a back effect of the early formed if onto the inducing virus. a factor which stimulates viral if interferon induction also in young cultures, is released from age ... | 1975 | 164104 |
| relationship between fluorescence and conformation of epsilonnad+ bound to dehydrogenases. | this work reports on the interaction of the fluorescent nicotinamide 1,n6-ethenoadenine dinucleotide (epsilonnad+) with horse liver alcohol dehydrogenase, octopine dehydrogenase, and glyceraldehyde-3-phosphate dehydrogenase from different sources (yeast, lobster muscle, and rabbit muscle). the coenzyme fluorescence is enhanced by a factor of 10-13 in all systems investigated. it is shown that this enhancement cannot be due to changes in the polarity of the environment upon binding, and that it m ... | 1975 | 164204 |
| binding of au(cn)2- and pt(cn)4-2- to horse liver alcohol dehydrogenase. a 35c1nmr relaxation study. | the binding of au(cn)2- and pt(cn)4-2- ions to the coenzyme binding site of horse liver alcohol dehydrogenase (alcohol : nad+ oxidoreductase ec 1.1.1.1) has been studied by 35c1 nuclear magnetic relaxation. longitudinal relaxation rates were analyzed in terms of a simple model and binding constants for au(cn)2-, pt(cn)4-2- and c1- were estimated. from a comparison between transverse and longitudinal relaxation rates the correlation time and the quadrupole coupling constant of bound chloride ion ... | 1975 | 164218 |
| time relationships in hormone responses: increased ovarian lactic acid production in vitro and estrogen production in vivo in immature female rats treated with pms. | | 1975 | 164254 |
| formation of steroids by the pregnant mare. v. metabolism of 14c-isopentenylpyrophosphate and 3h-dehydroisoandrosterone injected into the fetus. | a mixture of 1-14c-isopentenylpyrophosphate and 3h-dehydroisoandrosterone was injected into a horse fetus intramuscularly during laparotomy, after which maternal urine was collected for 4 days. steroid conjugates in the urine were extracted with amberlite xad-2 resin, hydrolysed and separated into phenolic and neutral fractions. from the phenolic fraction estrone, 17alpha-estradiol, equilin and equilenin were isolated. only estrone and 17alpha-estradiol contained both 3h and 14c, while the ring ... | 1975 | 164338 |
| preovulatory changes of ovarian cyclic amp in the rat. | | 1975 | 164595 |
| binding of human chorionic gonadotropin by rat ovarian slices: dependence on the functional state of the ovary (38571). | ovarian receptors specific for hcg-lh can be induced by pmsg-ovine lh priming. the marked increase in binding activity was accompanied by a rise in ovarian progesterone concentration and preceded by ovarian weight increase. the high binding activity of heavily luteinized ovaries was followed by a decline to prepriming levels during luteolysis. the ovarian progesterone content was decreased simultaneously and no change in ovarian weight was evident. the data demonstrate that ovarian hcg bindin ... | 1975 | 164671 |
| equine infectious anaemia in czechoslovakia. | | 1975 | 164712 |
| immunity and the level of neutralization antibodies in foals and mares vaccinated with a modified live-virus rhinopneumonitis vaccine. | foals (farms 1 and 2) and mares (farms 1 and 3) vaccinated with a commercially available rhinopneumonitis vaccine were tested for the level of antibodies and for protection against the natural infection. the serum-neutralization (sn) antibody titers against equine herpesvirus type 1 were 1:8 or less in the majority of foals and 1:16 or higher in about 10%. the level of the nasal secretory antibody in the foals (farm 2) was less than 1:4. the vaccinated foals did not show any apparent respiratory ... | 1975 | 164808 |
| the use of steady-state treatment in the rapid kinetics of horse liver alcohol dehydrogenase. the effect of addition of product on the "burst" reaction. | | 1975 | 164825 |
| electron spin resonance study on peroxidase- and oxidase-reactions of horse radish peroxidase and methemoglobin. | | 1975 | 164829 |
| mechanism of viral persistence in equine infectious anemia. | | 1975 | 165036 |
| conformational studies of equilibrium structures in fragments of horse heart cytochrome c. | ultraviolet absorption and circular dichroism studies have been carried out on horse heart apo-cytochrome c and heme-free peptide fragments obtained by cyanogen bromide cleavage of the native protein. it was noted that the various peptides assume predominantly an unordered conformation in water solution. increasing ionic strength and addition of 2-chloroethanol increase the right-handed helical content. guanidinium hydrochloride favors the coil state. it was also demonstrated that two non-intera ... | 1975 | 165078 |
| the importance of picornavirus infections in respiratory disease of man and other mammals. | picornaviruses may be divided, by physicochemical properties, into enteroviruses, cardioviruses, caliciviruses, rhinoviruses and foot-and-mouth diseases viruses. although the respiratory tract may be the primary site of entry and multiplication for enteroviruses, cardioviruses and fmd viruses, few agents in these groups cause respiratory disease. a notable exception is coxsackievirus a21 which is an important cause of upper respiratory tract diseases in military recruits. the picornaviruses whic ... | 1975 | 165133 |
| occurrence of nervous-tissue tumors in cattle, horses, cats and dogs. | from 11 north american veterinary university hospitals and clinics, 248 animals were a confirmed diagnosis of nervous-tissue tumor were identified; 7 tumors were found in cattle, 28 in horses, 14 in cats, 199 in dogs, and none in other species. tumors were divided for analysis into three categories-glial, meningeal, and peripheral nerve. in cattle and horses, all tumors involved peripheral nerves, the risk of which, in horses, reached a plateau at 4-6 years of age and remained constant thereafte ... | 1975 | 165149 |
| transformation of horse skin cells by type-c sarcoma viruses. | a horse skin cell line (e. derm, nbl-6, ccl-57) was susceptible to focus formation by the kirsten mouse sarcoma virus, feline sarcoma virus (st stain) and the msv pseudotypes with woolly monkey, gibbon monkey, rd-114, at-124, baboon placenta and murine xenotropic (balb/c 3t3 and c57l/jd) type-c viruses. foci were detected within 5 days after infection and the transformed cells continued to produce infectious virus and group-specific antigen of their respective type-c leukemia viruses. the transf ... | 1975 | 165152 |
| specific immunity and nonspecific resistance to infection: listeria, protozoa, and viruses in mice and hamsters,. | specific immunity developed by mice against protozoan (toxoplasma gondii and besnoitia jellisoni) and bacterial (listeria monocytogenes) infections was compared with nonspecific protection conferred by prior infections. the results indicated that homologous immunity protected mice from more than 10-5 ld50 of t. gondii or b. jellisoni, but from only 10-2 ld50 of l. monocytogenes. heterospecific protection among these organisms was for 10-0.4 minus 10-1.2 ld50. in studies in hamsters specific immu ... | 1975 | 165241 |
| distribution of delta-5 -3beta-hydroxysteroid dehydrogenase activity in the graafian follicle of the sheep. | the localization of delta-5 -3beta-hydroxysteroid dehydrogenase (3 beta-hsd) has been examined in ovarian follicles in vivo and in vitro, and related to oestrogen and progesterone production. in vivo, during the oestrous cycle, enzyme activity was restricted to the theca interna of the one or two most advanced follicles in each animal, but was present only between day 2 and 5 and between day 13 and ovulation. high levels of oestrogen were found in the ovarian venous blood only when follicles con ... | 1975 | 165292 |
| serologic characterization and sero-epidemiologic studies on acute hemorrhagic conjunctivitis (ahc) virus. | serologic and sero-epidemiologic characteristics of ahc virus infection were studied by neutralization test (nt). four-fold or greater virus neutralizing (vn) antibody response was demonstrated to the japanese isolate of ahc virus (the j 670/71 strain) in 77.3% and 66.7% of paired sera from clinical ahc patients in japan (1971-1973) and tunisia (1973). the four patients from indonesia studied in 1972 showed similar antibody response. cross-neutralization tests of ahc virus isolated in japan (197 ... | 1975 | 165719 |
| an examination of octanol and octanal metabolism to octanoic acid by horse liver alcohol dehydrogenase. | the kinetics of the horse liver alcohol dehydrogenase (alcohol: nad+ oxidoreductase ec 1.1.1.1) catalyzed metabolism of octanol and octanal to octanoic acid have been examined. on incubation of octanol with horse liver alcohol dehydrogenase in the presence of nad+, nadh as well as octanal and octanoic acid were seen as the initial products. however, on continued incubation, the octanal concentration progressively decreased to where only negligible quantities were present in the incubation after ... | 1975 | 165828 |
| spectroscopic properties of ferrous heme complexes of sterically hindered ligands. | mesoheme ix complexes of sterically hindered ligands 2-methylimidazole, tert-butylamine and 2-methylpyridine in aqueous glycerol solutions are characterized by broad visible absorption spectra at ambient temperature exhibiting close similarities to high-spin ferrous hemeproteins. spectrophotometric titrations of mesoheme ix with these ligands indicate well-defined equilibria for 2-methylimidazole and tert-butylamine corresponding to the formation of penta-coordinate strong-field ligand complexes ... | 1975 | 165836 |
| comparison of the effects of ethinyl oestradiol and conjugated equine oestrogens in oophorectomized women. | seventeen oophorectomized women were treated for 3 month periods, in random sequence, with ethinyl oestradiol 20 and 50 mug daily and conjugated equine oestrogens (premarin)0-625 and 1-25 mg daily. the serum cholesterol, clot lysis time, plasma fibrinogen, platelet adhesiveness and activated partial thromboplastin time remained unchanged throughout the different oestrogen regimes. there was a significant rise of serum triglyceride levels on both doses of ethinyl oestradiol but no significant cha ... | 1975 | 165913 |
| assignment of aromatic amino acid pmr resonances of horse ferrocytochrome c. | | 1975 | 165969 |
| the crystal structure of bonito (katsuo) ferrocytochrome c at 2.3 a resolution. ii. structure and function. | the structure analysis of bonito heart ferrocytochrome c was carried out at 2.3 a resolution by x-ray diffraction, and a kendrew-type skeletal model was built up. this molecule has an overall egg shape, 35 a in height, 30 a in width and 23 a in thickness; the 5th ligand of the heme iron atom is the n-epsilon atom of the his-18 imidazole ring and the 6th is the met-80 sulfur atom. distinct alpha-helix regions are found between the n-terminus and reside 11, between 60 and 69, and between 90 and th ... | 1975 | 166072 |
| [new immunization schedule against equine influenza]. | | 1975 | 166531 |
| paramagnetic spin label interactions with the envelope of a group a arbovirus. lipid organization. | electron paramagnetic resonance observations were made on nitroxide spin- labeled molecules which were bound to the tc-83 vaccine strain of venezuelan equine-encephalomyelitis virus. paramagnetic resonance parameters derived from the observations and their dependence on sample temperature were similar but not identical to those which have been reported for these labels dissolved in lipid bilayer membranes of mammalian and bacterial origin. the data has a mechanical rigidity substantially greater ... | 1975 | 166687 |
| isolation of equine herpesvirus type 1 from a horse with an acute paralytic disease. | a standardbred mare became paralyzed shortly after showing signs of an upper respiratory infection. the mare was euthanized and equine herpesvirus type 1 was isolated from the brain and spinal cord. | 1975 | 166741 |
| adenoviral pneumonia in a foal. | a three-week-old arabian filly was admitted to the large animal hospital with a respiratory disorder and died despite symptomatic treatment. the necropsy lesions were suggestive of viral pneumonia. an equine adenovirus were isolated from nasal and pharyngeal swabs and from several tissues after death. typical adenovirus virions were demonstrated by electron microscopy. | 1975 | 166797 |
| assay of lactogenic hormones using receptors isolated from rabbit liver. | using 125-i-labelled ovine prolactin and receptors isolated from the livers of rabbits, a sensitive method has been developed suitable for the assay of ovine, bovine, porcine, human and rat prolactins. these hormones showed competitive displacement of 125-i-ovine prolactin which was in general agreement with their respective activities in the pigeon crop sac bioassay. human and monkey growth hormones and human placental lactogen, which have marked prolactin-like actions on mammary tissue were al ... | 1975 | 166819 |
| the effect of lh on cyclic amp and progesterone in rat ovaries in vivo. | lh injected into the abdominal vein of immature female rats increased ovarian cyclec amp levels within 1 min, and the effect lasted at least 2 hr. in pms stimulated rats, a maximal effect on cyclic amp levels was obtained with 5 mug of lh 10 min after injection. significant effects were obtained with as little as 0.1 mug. the minimum effective dose required to increase ovary cyclic amp levels in proestrus rats was 0.05 mug of lh. while 0.01 mug did not affect cyclic amp levels, an increase i ... | 1975 | 166820 |
| [inhibiting effects of anti-proteinase agents upon induced ovulation]. | | 1975 | 166893 |
| a serologic survey of pronghorns in alberta and saskatchewan, 1970-1972. | to determine the exposure of free-ranging pronghorns (antilocapra americana ord) to selected pathogens, serum samples were obtained from 33 live-trapped animals from southwestern saskatchewan in 1970, and from 26 and 51 animals from southeastern alberta, in 1971 and 1972, respectively. antibodies were found to the agents of parainfluenza 3, bovine virus diarrhea, eastern and western encephalomyelitis, infectious bovine rhinotracheitis and the chlamydial group. no serologic reactors were found to ... | 1975 | 167203 |
| the prevalence of equid herpes virus 2 infections. | | 1975 | 167501 |
| mammalian liver alcohol dehydrogenases. | literature on the properties of liver alcohol dehydrogenase (adh) from man, horse and rat is reviewed and discussed under two major headings: 1) physical and chemical properties of adh and 2) structure-function relationship in isoenzymes. under the first heading are discussed: molecular weight, subunit composition catalytic sites per molecule, sulfhydryl groups, end groups, amino acid composition, role of zn++ in the structure and function, coenzyme specificity and binding, conformational change ... | 1975 | 167554 |
| inhibition of the growth of hemadsorption 2 virus by three acyl derivatives of amino acids. | three acyl derivatives of amino acids, dicarbobenzoxy-l-lysine sodium, carbobenzoxy-l-aspartic acid-beta-benzyl ester potassium, and n-3-phenylpropionyl-s-benzyl-l-cysteine potassium inhibit the growth of parainfluenza 1 (hemadsorption 2) virus. the growth of simian virus 40, vaccinia, poliomyelitis type 1, semliki forest, eastern equine encephalitis, and western equine encephalitis viruses was not affected by these compounds. four other acyl derivatives of amino acids did not inhibit the growth ... | 1975 | 167659 |
| the role of arginyl residues in directing carboxymethylation of horse liver alcohol dehydrogenase. | the selective carboxymethylation by iodoacetate of cys-46 in the active center of horse liver alcohol dehydrogenase has been shown to be mediated by interaction of the anionic reagent with the arginyl residue(s) previously shown to be responsible for binding nadh (l.g. lange, j.f. riordan, and b.l. vallee (1974), biochemistry 13, 4361). thus, sequential and reversible chemical modification of arginine with butanedione and of cysteine with pmercuribenzoate demonstrate that the essential thiol gro ... | 1975 | 167828 |
| studies on the heme environment of horse heart ferric cytochrome c. azide and imidazole complexes of ferric cytochrome c. | horse heart ferric cytochrome c was investigated by the following three methods: (i) light absorption spectrophotometry at 23 degrees c and 77 degrees k; (ii) electron paramagnetic resonance (epr) spectroscopy at 20 degrees k; (iii) precise equilibrium measurements of ferric cytochrome c with azide and imidazole between 14.43 and 30.90 degrees c. i and ii have demonstrated that: (1) ferric cytochrome c azide and imidazole complexes were in the purely low spin state between 20 degrees k and 23 de ... | 1975 | 167834 |
| the mechanism of ferrocytochrome c oxidation by a horseradish isoperoxidase. | the oxidation of ferrocytochrome c catalysed by highly purified horse-radish isoperoxidase p2 was studied kinetically. to take into account the low turnover number of the enzyme and the tendency to autocatalytic oxidation of ferrocytochrome c, experimental conditions were used which prevented us from using the steady-state treatment. according to kinetic results reported by several authors, a kinetic scheme involving a ternary complex between the enzyme and the substrates was postulated and simu ... | 1975 | 167872 |
| kinetic equivalence of the active sites of alcohol dehydrogenase from horse liver. | the reduction, catalysed by liver alcohol dehydrogenase, of benzaldehyde in the presence and absence of pyrazole, and the oxidation of benzyl alcohol and cyclohexanol in the presence of isobutyramide, has been measured by the stopped-flow technique. in performing these experiments particular care was taken to purify the enzyme, coenzymes, substrates and inhibitors, and to minimise as much as possible the effects of a blank substrate reaction. the calculation of the amount of substrate converted ... | 1975 | 168081 |
| effect of steroid hormones on growth of adrenocortical carcinoma transplants in mice;. | the growth of transplanted carcinomas originating in a gonadectomized c3h male mouse was much faster in intact male mice than in intact or gonadectomized females. administration of 17beta-estradiol or testosterone propionate in pellets resulted in a marked acceleration of the carcinoma growth in intact female mice. progesterone and deoxycorticosterone acetate exerted no influence on the carcinoma growth. neither gonadotropins (pregnant mare's serum gonadotropin plus human chorionic gonadotropin) ... | 1975 | 168115 |
| reactivity of serum from rous-sarcoma-bearing chickens with autochthonous and with allogeneic tumor cells: preferential autochthonous recognition. | complement-medicated cytotoxicity and indirect immunofluorescence assays showed that sera from random-bred chickens bearing rous sarcoma (rs) tumors are usually more reactive in vitro against rs cells of autochthonous than of allogeneic origin. absorption of such sera with autochthonous, but not with allogeneic, rs cells abrogated the fluorescence staining capacity. sera from tumor-bearing birds did not stain normal chicken embryo cells (cec), but reacted with a higher proportion of cec transfor ... | 1975 | 168159 |
| analysis of specificity of poliovirus inhibitors with inhibitor-resistant mutants of a strain of type 1 poliovirus. | attempts were made to analyze the specificity of inhibitory activities of normal bovine and equine sera to the mahoney strain of type 1 poliovirus. a total of five inhibitory factors were postulated to explain the complicated results. two of the three bovine inhibitors were identical in specificity to certain equine inhibitors despite differences in their mode of virus inactivation and their molecular size. in addition to this, inhibitors that could inactivate certain resistant mutants, but not ... | 1975 | 168261 |
| effect of conjugated oestrogen therapy on circulating luteinising hormone in oophorectomised women. | in a double-blind cross-over study, conjugated equine oestrogen (premarin) was shown to depress the raised luteinising hormone concentration found in the plasma of oophorectomised women. a dose of 1,25 mg was as effective as 2,5 mg per day. | 1975 | 168645 |
| universality of gonadotropic hormone effect. effect of mammalian fsh on the ovotestis of the snail helix pomatia. | gonadotropic hormone (fsh) stimulated markedly spermatogenesis in the ovotestis of snails. this is indicative of the presence of receptors sensitive to gonadotropic hormone in snail spermatogonia. fsh had no influence on oogenesis. | 1975 | 168707 |
| studies of individual carbon sites of proteins in solution by natural abundance carbon 13 nuclear magnetic resonance spectroscopy. relaxation behavior. | the aromatic regions in proton-decoupled natural abundance 13c fourier transform nuclear magnetic resonance spectra (at 14.2 kg) of small native proteins contain broad methine carbon bands and narrow nonprotonated carbon resonances. some factors that affect the use of natural abundance 13c fourier transform nmr spectroscopy for monitoring individual nonprotonated aromatic carbon sites of native proteins in solution are discussed. the effect of protein size is evaluated by comparing the 13c nmr s ... | 1975 | 169239 |
| studies of individual carbon sites of proteins in solution by natural abundance carbon 13 nuclear magnetic resonance spectroscopy. strategies for assignments. | natural abundance 13c fourier transform nmr spectra (at 15.18 mhz, in 20-mm sample tubes) of aqueous native proteins yield numerous narrow single carbon resonances of nonprotonated aromatic carbons. techniques for the assignment of these resonances are presented. each technique is applied to one or more of the following proteins: ferricytochrome c from horse heart and candida krusei, ferrocytochrome c and cyanoferricytochrome c from horse heart, lysozyme from hen egg white, cyanoferrimyoglobins ... | 1975 | 169240 |
| mitochondrial and herpesvirus-specific deoxypyrimidine kinases. | to characterize and compare the thymidine (tdr) and deoxycytidine (cdr) kinase isozymes of uninfected and herpesvirus-infected cells: (i) the subcellular distribution of the isozymes has been studied; (ii) a specific assay for cdr kinase has been devised; (iii) the tdr kinase isozymes have been partially purified; and (iv) the purified enzymes have been analyzed by disc polyacrylamide gel electrophoresis, isoelectric focusing, and glycerol gradient centrifugation and by substrate competition and ... | 1975 | 169387 |
| the chemotaxis of selected cell types to connective tissue degradation products. | because rheumatoid inflammation is associated with the presence of large numbers of leukocytes in joint effusions, the question of whether enzymatic splitting of collagen and fibrin can lead to generation of chemotactic factors was investigated. fibrinogen was purified from the plasma of four different species, and the homogeneity of the preparations was established by physicochemical and immunologic techniques. fibrin was prepared and then lysed with plasmin to obtain fibrin degradation product ... | 1975 | 169719 |
| inheritance of a lethal immunodeficiency disease of arabian foals. | a fatal syndrome of certain arabian foals which begins at about 25 days of age (range 14 to 46 days) and which runs a course of about 23 days (range 13 to 42 days) is described. the syndrome, which affected 17 foals on a single farm is further characterised by pneumonia, in some instances by dermatitis (dermatophilosis) and other infections, together with a progressive decline in health till death at about 49 days of age (range 34 to 77 days), despite intensive therapy. four of the foals, on his ... | 1975 | 169784 |
| ibaraki disease and its relationship to bluetongue. | ibaraki disease, an epizootic disease of cattle in japan resembling bluetongue, is characterized by fever and lesions affecting the mucous membranes, the skin, the musculature and vascular system. degeneration of striated muscular tissue is observed in the oesophagus, larynx, pharynx, tongue and the skeletal muscles. oedema and haemorrhage are marked in the mouth, lips, abomasum, around the coronets, etc., and are occasionally followed by degeneration of the epithelium leading to erosions or ulc ... | 1975 | 169788 |
| conformational energy refinement of horse-heart ferricytochrome c. | the reported x-ray structure of horse-heart ferricytochrome c has been refined by conformational energy calculations, using a three-stage computational procedure. in stage i, the atomic positions are adjusted to conform to idealized bond lengths and bond angles characteristic of small amino acid derivatives, while yet remaining as close as possible to the x-ray coordinates. in stage ii, atomic overlaps are eliminated by adjusting the backbone and side-chain dihedral angles to minimize the nonbon ... | 1975 | 169878 |
| the influence of amino acid substitutions on the conformational energy of cytochrome c. | conformational energies have been evaluated for each of the staggered side-chain conformations associated with the 261 amino acid substitutions known to occur among 60 eucaryotic species. at least 86% of these substitutions can be sterically accommodated (one at a time) within the structure of horse-heart cytochrome c resulting from conformational energy refinement. simultaneous incorporation of all pertinent amino acid substitutions found in eight representative species into the refined horse-h ... | 1975 | 169879 |
| optical and magnetic resonance studies of formate binding to horse liver catalase and sperm whale myoglobin. | the binding of formate ion, a substrate for the peroxidatic reaction of catalase, has been investigated by magnetic resonance techniques. comparative studies of formate binding to ferric myoglobin have also been performed. the nuclear magnetic relaxation (nmr) rate of formate and water protons is enhanced by the presence of ferric horse liver catalase. the enhancement is not changed significantly by the addition of cyanide, indicating that water and formate are still bound in the presence of cya ... | 1975 | 169890 |
| carbon-13 nuclear magnetic resonance spectroscopy of [2-13c]carboxymethylcytochrome c. | horse heart cytochrome c has been carboxymethylated under various reaction conditions using [2-13c]bromoacetate. direct analysis of reaction products using 13c nuclear magnetic resonance spectroscopy shows that the protein can be much more extensively modified than has previously been assumed. the proximity of one carboxymethylmethionine residue to the paramagnetic center of the ferric protein allows it to be distinguished from a more constant carboxymethylmethionine residue on the basis of the ... | 1975 | 169897 |
| identification of multiple equine infectious anemia antigens by immunodiffusion reactions. | equine infectious anemia (eia) cell antigens prepared from infected equine spleen, equine leukocyte cultures or a persistently infected equine dermis cell line contained at least two serologically reacting components. for convenience one component was designated as soluble antigen (sa) and the other as cell-associated antigen (caa). the sa appeared as a single component when it was prepared from eia virus precipitated from infectious tissue culture fluid with polyethylene glycol and ether treate ... | 1975 | 169969 |
| viral susceptibility of a cell line derived from the pig oviduct. | seventeen of 24 rna viruses and eight of nine dna viruses replicated in a cell line derived from a pig fallopian tube. the following rna viruses grew poorly in it: the virus of transmissible gastroenteritis of pig and the swine-influenza, sendai and bovine para-influenza type 3 viruses. among other rna viruses an untyped swine para-myxovirus and some picornaviruses, rhabdoviruses and togaviruses attained high titers and produced an extensive cytopathic effect. among the dna viruses a porcine ade ... | 1975 | 169971 |
| an adrenal cell system suitable for long term study of factors affecting steroidogenesis. | cell suspensions of normal adult rad adrenals have been prepared by trypsinization and incubated in ham's nutrient mixture f10 containing horse serum, fetal calf serum, and lima bean trypsin inhibitor. in most experiments culture medium was not changed during incubation. in this system the number of cells fell to 50% after 2 days, then slowly declined to 20% after 1 month of incubation. a corticosterone (b) response was seen to as little as 5 muu of acth per millilitre, a concentration which is ... | 1975 | 169974 |
| preparation of an alcohol-dehydrogenase--nad(h)--sepharose complex showing no requirement of soluble coenzyme for its activity. | 1. horse liver alcohol dehydrogenase and an nadh analogue, n6-[(6-aminohexyl)carbamoylmethyl]-nadh, have been co-immobilized to sepharose 4b under conditions permitting binary complex formation between the enzyme and the cofactor. 2. the enzyme-coenzyme-matrix preparations were assayed with a coupled oxidoreduction reaction and showed activities, prior to addition of coenzyme, that were up to 40% of that obtained in excess of free coenzyme. 3. a molar ratio of 1:1 between the amount of bound enz ... | 1975 | 170114 |
| wildlife and environmental health: raccoons as indicators of zoonoses and pollutants in southeastern united states. | inasmuch as terrestrial fauna are an integral part of our natural environment and are directly exposed to disease and pollutants, it follows that certain wild populations could serve to detect subtle alterations within ecosystems. a collection of studies on raccoons is presented to stimulate other researchers to develop the potential of our wildlife resources as monitors of environmental health. raccoons have been used as serologic sentinels for st louis encephalitis and venezuelan equine enceph ... | 1975 | 170239 |
| [equine rhinopneumonitis virus infection in horses (author's transl)]. | | 1975 | 170706 |
| replication of equine herpesvirus type i: resistance to hydroxyurea. | | 1975 | 170739 |
| steroid oxidoreductase activity of alcohol dehydrogenases from horse, rat, and human liver. | alcohol dehydrogenase from horse (isoenzyme ss and es, but not ee), rat and human liver were found to catalyze the nad-dependent oxidation of 3beta-hydroxy groups in 5alpha- and 5beta-steroids of the c19, c21, and c24 series. the enzymes from horse and rat liver were more active on 5beta-than on 5alpha-steroids. this difference was most marked with the enzyme from rat liver, especially with 3beta-hydroxyandrostan-17-ones and 3beta-hydroxypregnan-20-ones as substrates. the km of isoenzyme es from ... | 1975 | 170765 |
| estradiol receptor content in rat granulosa cells during follicular development: modification by estradiol and gonadotropins. | to examine the relationships between gonadotropic hormones, ovarian follicular development, and estradiol receptor content in rat granulosa cells, follicular growth was stimulated in intact immature rats and hypophysectomized immature rats. in intact rats, both pmsg and ofsh given for 2 days stimulated follicular growth and endogenous estrogen production but caused estradiol receptor content in granulosa cells to decrease markedly. in hypophysectomized rats estradiol (2 mg/day x 4) was capable o ... | 1975 | 171146 |
| sensitivities of neurotropic arboviruses to human interferon. | the sensitivities of selected neurotropic arboviruses (st. louis encephalitis, western equine encephaltis, and la crosse viruses) to human interferon and polyriboinosinic-polyribocytidylic acid (poly i-poly c) were determined in primary human fetal glial cell cultures. the sensitivities were measured by an end point (inhibition of virus yield). all of the viruses were sensitive to human interferon and poly i-poly c; st. louis encephalitis and western equine encephalitis viruses were inhibited at ... | 1975 | 171314 |
| a prospective evaluation of heterophile and epstein-barr virus-specific igm antibody tests in clinical and subclinical infectious mononucleosis: specificity and sensitivity of the tests and persistence of antibody. | three heterophile antibody tests and a test specific for igm antibody to epstein-barr virus were evaluated during prospective studies of infectious mononucleosis. specificity was judged by the frequency of false-positive results in sera of known qualities taken before illness; except for two patients bled during early, unrecognized illnes,, titers of greater than or equal to 1:40 were detected in 12% by the absorbed sheep red cell test, in 6.7% by the absorbed horse red cell test, and in none by ... | 1975 | 171321 |
| the effect of inhibition of prostaglandin synthesis on ovarian hypertrophy in the rat. | aspirin and indomethacin, inhibitors of prostaglandin biosynthesis, were utilized to determine the role of prostaglandins (pgs) in ovarian weight gain in rats following unilateral ovariectomy or treatment with pmsg. after unilateral ovariectomy, the compensatory ovarian hypertrophy was 185-0% compared with 139-8% and 97-5% in rats treated with indomethacin and aspirin, respectively. the adrenal weights in rats treated with aspirin were also reduced significantly. administration of pge2 or pgf2al ... | 1975 | 171401 |
| murine oncornavirus activation in the pancreas during infection with venezuelan equine encephalitis virus. | electron microscopic studies of the pancreases of 3-week-old icr swiss mice infected with venezuelan equine encephalitis virus and killed 6 days post inoculation (at which time they were moribund) indicated significantly more type-c particles than were found in uninfected controls. this phenomenon was apparently possible because of the microanatomy of the pancreas, in which interstital spaces allowed accumulation of virus particles. | 1975 | 171430 |
| virus-induced abortion. studies of equine herpesvirus 1 (abortion virus) in hamsters. | a hamster-adapted strain of equine herpesvirus-1 (equine abortion virus) caused severe hepatic degeneration in both pregnant and nonpregnant hamsters and, in addition, regularly induced abortion in pregnant hamsters inoculated at midgestation. in nonpregnant hamsters, the only consistently affected organ was the liver despite a prolonged viremia. newborn animals usually died 1 to 2 days after inoculation; adults died 5 to 9 days after inoculation. in pregnant hamsters, the virus had a tropism fo ... | 1975 | 171478 |
| treatment for debilitated horses. | | 1975 | 171552 |
| efficacy of 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate in therapy of equine abortion virus-induced hepatitis in hamsters. | equine abortion virus (eav)-induced hepatitis in hamsters presents an interesting animal model for the evaluation of drugs possessing anti-deoxyribonucleic acid virus activity. these experiments demonstrate that 9-beta-d-arabinofuranosylhypoxanthine 5'-monophosphate (ara-hxmp), a new synthetic, water-soluble, antiviral agent, effectively controls this disease in hamsters with a therapeutic index of approximately 60. ara-hxmp prevented hepatitis-associated deaths in hamsters, reduced the titer of ... | 1975 | 172009 |
| activation of liver alcohol dehydrogenases by imidoesters generated in solution. | various omega-halogenated carboxy acids and amides were evaluated as potential active-site-directed reagents for alcohol dehydrogenase. 2-bromoacetamide and bromoacetic and 3-bromopropionic acids inactivated the enzyme; amp, nad+, and nadh markedly decreased the rate of inactivation. some omega-halogenated carboxyamides, x(ch2)nconh2, increased the activity of the enzyme with the rate and extent of activation depending on the number of methylene units (n) in the order 3 greater than 4 greater th ... | 1975 | 172120 |
| size and charge heterogeneity of rat tissue ferritins. | ferritins purified from horse spleen and from rat liver, kidney, heart and hepatoma were analyzed by quantitative polyacrylamide gel electrophoresis. from the migration characteristics of these ferritins at several gel concentrations, ferguson plots were constructed and the molecular sizes and charges (apparent valences) together with their statistical variability were obtained by applying rodbard computer programs to the data. finally, ellipses were drawn describing the 95% confidence limits of ... | 1975 | 172145 |