| antibodies reactive with leptotrichia buccalis in human serum from infancy to adulthood. | | 1976 | 8029 |
| ph changes during culture of human dental plaque streptococci on mitis-salivarius agar. | | 1976 | 8030 |
| the influence of organic phosphates on the bohr effect of human hemoglobin valency hybrids. | the bohr effect of hemoglobin and that of the aquomet and cyanomet valency hybrids was measured in the presence and the absence of ihp (inositol hexaphosphate) and dpg (2,3-diphosphoglycerate). in the absence of these organic phosphates the four hybrids show similar, but suppressed bohr effects as compared to hemoglobin. addition of ihp and dpg results in all cases in an increase of the bohr effect. the additional phosphate induced bohr effect of the hybrids with the alpha chain in the oxidized ... | 1976 | 7327 |
| isolation and properties of alpha-d-mannosidase from human kidney. | alpha-d-mannosidase activity exists in three forms that can be separated by deae-cellulose chromatography, alpha-d-mannosidase was isolated from human kidney in a homogeneous state, and was purified 2100-fold, with p-nitrophenyl alpha-d-mannoside as substrate. the purified alpha-d-mannosidase was practically free from all other glycosidases tested. the km of the synthetic substrate with the enzyme was 1 x 10(-3) m and the ph optimum 4.5. it was inhibited by heavy metals, sodium dodecyl sulphate, ... | 1976 | 7243 |
| general pharmacological properties of a new non-opiate antitussive: zipeprol (3024 cerm). i. action on respiratory function and acute toxicity. | 1-(2-methoxy-2-phenyl)-ethyl-4-(2-hydroxy-3-methoxy-3-phenyl)-propyl-iperazine-dihydrochloride (zipeprol, respilene) is a substance of non-phenanthrenic chemical structure. in the cat, it antagonised cough induced by stimulation of the superior laryngeal nerve or by direct mechanical excitation of the sensitive tracheo-bronchial receptors. the efficacy of zipeprol after enteral administration made it possible both to establish good intestinal absorption and to rank it favourably in relation to s ... | 1976 | 8057 |
| actions of narcotics on brain dopamine metabolism and their relevance for "psychomotor" effects. | a review is given about the effects of narcotic analgesics, particularly of morphine, on the dopamine metabolism in the corpus striatum and about the relations of these effects to motility and "psychomotor" phenomena. in rats, acute doses of morphine decrease the dopaminergic neurotransmission in brain, without blocking postsynaptic dopamine receptors. chronic treatment of rats with morphine reverses these acute effects of morphine and induces symptoms of an increased dopaminergic neurotransmiss ... | 1976 | 8059 |
| metabolic deacetylation: in vitro and in vivo studies in man, rat, dog and rhesus monkey with isomeric tetrahydroisoquinolyl derivatives of 3,4-dimethylbenzyl acetate. | the pharmacological activities of a racemic mixture of tetrahydroisoquinolyl derivatives of 3,4-dimethylbenzyl acetate, (+/-) ro 03-4661 and the corresponding resolved isomers (+) ro 03-4661 and (--) ro 03-4661 have been studied in rat and rhesus monkey. the racemate and the (--) isomer showed narcotic analgesic activity by the oral route in both species. drug metabolism studies indicated that the activity was probably due to metabolic deacetylation to the corresponding carbinol ro 03-4632. deac ... | 1976 | 8060 |
| purification and characterization of lipoprotein lipase from human heart. | human heart lipoprotein lipase was purified by affinity chromatography on heparin-sepharose 4b. when crude extracts of heart acetone powder were applied to columsn, about 40% of total lipase activity was bound to the gel and then eluted with 1.5 m nacl. at this stage the eluted enzyme was purified 1900-fold. disc gel electrophoresis yielded a single protein band corresponding with lipolytic activity. minimum molecular weight of the protein was 60,000 as determined by polyacrylamide gel electroph ... | 1976 | 8061 |
| kinetic study of the action of snake venom phospholipase a2 on human serum high density lipoprotein 3. | the hydrolysis of the phospholipids of intact human serum high density lipoprotein 3 (hdl3) by pure alpha-phospholipase a2 from crotalus adamanteus was studied by ph-stat titration. the enzyme quantitatively hydrolyzed phosphatidylcholine and phosphatidylethanolamine and left sphinogomyelin intact, yielding a stable and water-soluble modified hdl. lysophospholipids and free fatty acids, the products of hydrolysis, remained in the lipoprotein. when 1 mol of defatted bovine serum albumin/mol of su ... | 1976 | 5436 |
| neutral proteinases of human spleen. purification and criteria for homogeneity of elastase and cathepsin g. | 1. human spleen was found to contain proteinases active against azo-casein at neutral and alkaline ph values. 2. the activity was stimulated by high ionic strength and some detergents. 3. optimal extraction of the proteinases from the tissue was achieved with 1.0m-nacl containing 0.1% brij 35 and 0.1% trisodium edta. 4. the proteinases were efficiently adsorbed to insoluble material in the absence of salt in the initial stages of purification. 5. two distinct proteinases were separated by chroma ... | 1976 | 7244 |
| human cathepsin g. catalytic and immunological properties. | 1. the specificity of cathepsin g, a neutral proteinase from human spleen, was examined by use of low-molecular-weight substrates. the enzyme was found to hydrolyse several synthetic substrates also hydrolysed by chymotrypsin, but with different kinetic constants. 2. maximal activity against benzoyl-dl-phenylalanine 2-naphthol ester and azo-casein was in the range ph 7.5-8.0. 3. the sensitivity of cathepsin g to the action of potential inhibitors was determined, and compared with those of bovine ... | 1976 | 7245 |
| milk protein quantity and quality in low-birthweight infants: i. metabolic responses and effects on growth. | the optimal quantity and quality of protein for low-birthweight infants is undefined. in this study, 106 well, appropriate-for-gestational age, low-birthweight infants weighing 2,100 gm or less were grouped in three gestational age categories: t1 = 28 to 30 weeks; t2 = 31 to 33 weeks; t3 = 34 to 36 weeks. each group was assigned randomly to either banked human milk (bm) or to one of four isocaloric formulas varying in quantity and quality of protein but not in mineral content or in fat content: ... | 1976 | 7767 |
| gamma-glutamyl transpeptidase, a lymphoid cell-surface marker: relationship to blastogenesis, differentiation, and neoplasia. | gamma-glutamyl transpeptidase, an enzyme that catalyzes gamma-glutamyl transfer from gamma-glutamyl compounds to amino acid and peptide acceptors, and which is known to be localized in the membranes of many epithelial cells, was found in a variety of lymphoid cells. the lymphoid cell enzyme is located on the cell surface, and exhibits substantially the same substrate specificity as the enzyme found in epithelial cells. human and rat (but not mouse) lymphocyte gamma-glutamyl transpeptidase was st ... | 1976 | 7785 |
| prolactin secretion by metoclopramide in man. | six men and nine women were given intravenous injections of 2.5 mg of metoclopramide to assess its potential as a stimulus to prolactin release. following the administration of metoclopramide, there was prompt increase in serum prolactin to a peak response of 38.2 +/- 3.9 ng/ml in men and 103 +/- 10.2 ng/ml in women. the prolactin response to metoclopramide in men was compared with the response to 400 mug of trh in 10 men. the peak response after trh was 22.4 +/- 2.2 ng/ml, which was significant ... | 1976 | 7571 |
| estimation of the degree of binding of tetracyclines in human plasma. | | 1976 | 7652 |
| properties of fcgamma receptors in normal and malignant human tissues. | properties of the igg receptors were studied by treating tissue sections or suspensions of peripheral mononuclear cells with various chemical reagents and determining changes in their ability to react with igg-sensitized erythrocytes (ea). a heterogeneity of the fc receptors was revealed. iodoacetamide, 2-mercaptoethanol, sodium azide, edta, and a ph varying from 6.0 to 8.2 had no effect on fc receptors in sections of normal lymphoreticular tissue and malignant tissue. formaldehyde, low ph, and ... | 1976 | 7834 |
| studies on camel hemoglobin. 1. physico-chemical properties and some structural aspects of camel hemoglobin (camelus dromedarius). | hemoglobin from an adult camel (camelus dromedarius) was prepared from the red cell lysate by cm- and deae-cellulose chromatography. the purified hemoglobin showed a lesser mobility on starch gel electrophoresis at ph 8.5 than that of human hemoglobin c. native camel hemoglobin contains 95-99% alkali-resistant hemoglobin and in soluble in 2.94 m k2hpo4/kh2po4 buffer. different forms of camel hemoglobin show similar ammonium sulfate precipitation curves. indirect evidence for the stability of cam ... | 1976 | 7305 |
| ribonuclease activity in preparations of human leukocyte interferon. | preparations of human leukocyte interferon obtained by multi-stage purification procedure exhibited ribonuclease activity with the optimum at ph 7.0--7.5. the enzyme possessed the endonuclease action mechanism. most substances studied for their effect on the rna-ase activity in human interferon preparations showed many of them to act on the enzyme in the same way as on other ribonucleases. however, dithioerythritie, a reducing agent for disulfide bounds, activated the ribonuclease in the interfe ... | 1976 | 8177 |
| postsplenectomy sepsis due to influenzal viremia and pneumococcemia. | a 31-year-old man, who had undergone splenectomy 18 months previously because of hereditary spherocytosis, suddenly became ill, with fever, vomiting, epigastric pain and shock, and died 10 hours after the onset of his symptoms. autopsy showed influenzal viremia, pneumococcemia and bilateral adrenal hemorrhage. the rapid course of the patient's illness emphasizes the serious risk of sepsis for individuals who have had a splenectomy. anti-influenza immunization in such patients should be considere ... | 1976 | 8205 |
| understanding neurotransmitters and related drugs. | chemical neurotransmitter substances are released at the axon terminals of the central, autonomic, and peripheral nervous systems of the human body. the most well-known of the neurotransmitters are acetylcholine, norepinephrine, dopamine, and serotonin. it is these substances that facilitate the conduction of nerve impulses throughout the body, allowing the coordination of body functions and enabling response to the environment. the efective action of neurotransmitters makes the difference betwe ... | 1976 | 8207 |
| duodenal immunoglobulin deficiency in graft versus host disease (gvhd) mice. | the small intestine is a well documented target organ in mouse and human gvhd, and diarrhea is a prominent part of the clinical gvhd syndrome. although a plethora of systemic immune deficits has been documented in gvhd, the integrity of the small intestinal immune system has not been investigated. a correlation has not been demonstrated between systemic immune dysfuction and the incidence of lymphomas in mouse gvhd survivors. if gastrointestinal immune deficiency exists in mouse gvhd, its possib ... | 1976 | 7629 |
| calcium-independent modulation of cyclic gmp and activation of guanylate cyclase by nitrosamines. | nitrosamines markedly increase concentrations of guanosine 3', 5' - monophosphate (cyclic gmp) in several tissues from the rat and in human colonic mucosa. these agents are effective in the absence of extracellular calcium and enhance guanylate cyclase activity in tissue homogenates. stimulation of cyclic gmp was greatest in liver, where the carcinogenic activity of nitrosamines is also most pronounced. | 1976 | 7837 |
| ca++ binding properties of human prothrombin. | the binding of ca++ to human prothrombin has been investigated by equilibrium dialysis. the protein exhibited a positive cooperativity phenomenon for the first three ca++ bound. eleven to twelve ca++ binding sites have been found. they could be differentiated in terms of two classes of sites with respect to their ca++ affinity: 5 strong binding sites (log kassoc = 3.9) and 7 weak binding sites (log kassoc = 2.9). we attempted to determine the hill coefficient of the strong binding sites responsi ... | 1976 | 7324 |
| a comparison of the effect of flurbiprofen on prostaglandin synthetase from human rheumatoid synovium and enzymatically active animal tissues. | | 1976 | 7661 |
| technologic evolution in aeronautics and adaptation of man. | | 1976 | 7759 |
| cyclic amp and cyclic gmp in epidermal physiology and pathophysiology. | the second messengers cyclic amp and cyclic gmp in several organs appear to coordinate those molecular events which are responsible for specialized organ function. as a result of balanced cell proliferation and specialization, epidermis functions by terminal specialization which provides a barrier between man and environment. since the epidermal component of psoriasis is a classic example of deranged epidermal homeostasis, which has a low level of cyclic amp and a high level of cyclic gmp, it se ... | 1976 | 8278 |
| 5,5-bis(3-hydroxyphenyl)hydantoin, a minor metabolite of diphenylhydantoin (dilantin) in the rat and human. | 5,5-bis(4-hydroxyphenyl) hydantoin has been identified as a minor metabolite of diphenylhydantoin (dph) in the rat and human. this metabolite was synthesized in the laboratory from 4,4'-dihydroxybenzophenone. gas chromatographic and mass spectrometric comparison of the permethylated synthetic compound with the permethylated derivative of the metabolite obtained from biological sources showed that they were identical. the metabolite was excreted as a glucuronide and accounted for about 1% of the ... | 1976 | 8289 |
| metabolism of tripelennamine in man. | four polar metabolites were isolated from the urine of human subjects orally treated with tripelennamine, and their structures elucidated by various chemical and physical methods. one of the metabolites, which is a minor one, was identified as an n-oxide of tripelennamine, and the other three as glucuronide conjugates. one of the conjugates, which is a major metabolite, has been assigned a unique quaternary ammonium n-glucuronide structure, since it gave tripelennamine and d-glucuronic acid on i ... | 1976 | 8293 |
| disposition and metabolism of 3-(3-chlorophenoxy)-n-methylpyrrolidine 14c-carboxamide in the rat, dog, and man. | studies with a 14c-labeled sample have shown that the title compound a is readily absorbed and very rapidly excreted by the rat, dog, and man. the metabolites result from aromatic ring hydroxylation, oxidation, and dealkylation of the urea methyl group, and oxidative ring opening of the pyrrolidine ring. rat metabolites were 3-(3-chloro-4-hydroxyphenoxy)-n-methyl-l-pyrrolidinecarboxamide (c) 3-(3-chloro-4-hydroxyphenoxy)-1-pyrrolidinecarboxamide (d), 3-(3-chlorohydroxyphenoxy)-1-pyrrolidinecarbo ... | 1976 | 8294 |
| physiological disposition and metabolic fate of a new antiarrhythmic agent, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl) benzylamine in the rat, dog, monkey, baboon, and man. | the physiological disposition of a new orally active antiarrhythmic drug, alpha, alpha-dimethyl-4-(alpha, alpha, beta, beta-tetrafluorophenethyl)benzylamine (mk-251) was investigated in the rat, dog, rhesus monkey, baboon, and man. mk-251 was extensively absorbed after oral administration in all species. fecal excretion was the major route of tracer elimination in the rat (70%) and dog (80%), whereas the monkey, baboon, and man excreted the majority of the dose via the urine (40-80%). mk-251 and ... | 1976 | 8295 |
| studies of gamma-glutamyl transpeptidase in human ocular tissues. | | 1976 | 8323 |
| studies of various factors in thyrotropoin releasing hromone trh) radioimmunoassay for serum (author's transl). | to study the significance of trh in the hypothalamo-pituitary-thyroid axis measurement of trh in body fluid are needed. we previously reported trh radioimmunoassay for urine. trh radioimmunoassay for serum has not established yet, because trh immunoreactivity is inactivated with serum. we investigated effects of various factors on this inactivation and method for prevention of this inactivation. synthetic trh was added to normal human serum at 4 degrees c and incubated at 60 degrees c, 37 degree ... | 1975 | 8343 |
| liberation of an acid stable proteinase inhibitor from the human inter-alpha-trypsin inhibitor by the action of kallikrein. | | 1976 | 8369 |
| digestion of human immunoglobulin g by bovine cathepsin b1. | | 1976 | 8377 |
| isoniazid and iproniazid: activation of metabolites to toxic intermediates in man and rat. | acetylhydrazine, a metabolite of isoniazid, a widely used antituberculosis drug, and isopropylhydrazine, a metabolite of iproniazid, an antidepressant removed from clinical use because of high incidence of liver injury, were oxidized by cytochrome p-450 enzymes in human and rat liver microsomes to highly reactive acylating and alkylating agents. covalent binding of these metabolites to liver macromolecules paralleled hepatic cellular necrosis. the metabolites formed from these and probably other ... | 1976 | 7838 |
| structure-volume relationships in hemoglobin. a densitometric and dilatometric study of the oxy leads to deoxy transformation. | partial volume measurements have been performed for human hemoglobin, both on the oxygenated and deoxygenated forms. density measurements (by pycnometry) give vhbo2 = 0.752 +/- 0.002 and vhb =0.753 +/- 0.006 for the partial specific volume and do not distinguish between the two different structures. differential measurements, by dilatometry, however, show a signigicantly higher molal volume (of about 50 cm3/mol hemoglobin tetramer) for the deoxy over the oxygenated from at ph 7. the same reactio ... | 1976 | 7325 |
| alkaline phosphatase, arylsulfatase and beta-glucuronidase in saliva of cyclic women. | levels of the enzymes alkaline phosphatase, arylsulfatase, and beta-glucuronidase have been measured in whole unstimulated saliva samples collected daily during normal menstrual cycles. the cellular content of the saliva has been shown to be a major source of the three enzymes. the enzymes were found to have peak maximum levels of activity during the periovulatory -hase of the cycle. this observation can be attributed to the presence of increased numbers of exfoliated cells from the oral mucosa ... | 1976 | 8394 |
| partial regain of activity in heterologous recombinants of phosphorylcholine-binding m-components from different species. | recombination experiments were performed with heavy and light chains derived from a waldenström's igm with specificity against phosphorylcholine. the recombinant molecules had an association constant for phosphorylcholine in the same order of magnitude as the native igm; the number of binding sites at saturation was only slightly decreased in the reconstituted molecules, indicating regain of binding activity after recombination of igm heavy and light chains. heterologous recombinants obtained wi ... | 1976 | 7630 |
| neurotic and psychotic states attributed to thai "phii pob" spirit possession. | in thailand, the term "phii" refers to spirits and ghosts to which are generally attributed power over human beings. in the present, spirit possession is usually found in rural areas. however among several spirits, there is one particularly interesting spirit selected for this presentation, the phii pob. the "phii pob" is a common spirit in the northeast, north and some provinces in the central part of thailand (suwanlert, 1972). thai believers consider that the phii pob differs from other spiri ... | 1976 | 7225 |
| separation of slow reacting substance of anaphylaxis (srs-a) from human lung into four biologically active fractions. | slow reacting substance of anaphylaxis (srs-a) was released from human lung passively sensitized with ragweed antibody and challenged with specific antigen e. after purification by ethanol extraction, incubation with alkali (0.1 m naoh for 30 min at 37 degrees c) and chromatography on silicic acid and deae-cellulose, human srs-a was separated into four biologically active fractions (fractions i to iv). arylsulfatase (type h-1) in 0.1 m sodium acetate buffer, ph 4.5, destroyed the biologic activi ... | 1976 | 8568 |
| inhibition of dna synthesis in cultured human lymphocytes by phenylbutazone and oxyphenbutazone. | | 1976 | 8620 |
| correlation of in vitro and in vivo methodology for evaluation of antacids. | the rate and extent of acid consumption of an antacid suspension and tablet were evaluated by in vitro and in vivo techniques. four different test procedures were used to estimate in vitro antacid reactivity. in vivo effects were determined in the fasted and postcibal states in normal human subjects by a radiotelemetry procedure. the duration of elevation of intragastric ph greater than 3 was in agreement with in vitro estimates of total acid consumption of the antacid. there was also good corre ... | 1976 | 8626 |
| pulse radiolytic investigation of single heme group reduction in hum an methemoglobin. | reduction of one of the four heme groups of human aquomethemoglobin a has been investigated by the pulse radiolysis method. the reactivity of e-a-q, the hydrated electron, with methemoglobin was determined by observing this species directly. the separate reactions of the hydroxy yl radical and hydrogen atom, as well as of e-a-q, were studied by observing absorbance changes in the protein spectrum over the wavelength range 290 to 600nm, with appropriate scavengers in solution... | 1976 | 5439 |
| specific and non-specific effects of beta-adreno-receptor blocking drugs in man (author's transl). | the effect of tyramine infusion or exercise on catecholamine concentration and dopamine-beta-hydroxylase activity in plasma of normal volunteers has been studied. whereas the increase in plasma catecholamine concentrations by tyramine infusion was not changed 90 min after oral application of a single dose of beta-adrenoceptor blocking drugs (penbutolol, practolol, i.c.i. 66082), the increase in blood pressure was diminished. however, the increase in plasma catecholamine, concentration, i.e. the ... | 1976 | 8664 |
| studies on selenium-related compounds. v. cytogenetic effect and reactivity with dna. | five selenium compounds, na2se04, h2se04, na2se03, h2se03 and se02, were tested for their capacity to induce chromosome aberrations in cultured human leukocytes and for their reactivity with dna by a rec-assay system and inactivation of transforming activity in bacillus subtilis. chromosome-breaking activity was significantly higher for the compounds with four-valent than with six-valent selenium, the efficiency being in the decreasing order h2s03 greater than na2se03 greater than se02 greater t ... | 1976 | 8704 |
| purification and properties of the glucose-6-phosphate-dependent form of human placental glycogen synthase. | | 1976 | 8712 |
| the haemolytic effect of phallolysin. | phallolysin from the toadstool, amanita phalloides, is a basic protein that causes direct haemolysis of red cells. the dose-response curve is steep; the ph optimum is in the weakly acid range. the rate of haemolysis increases with the concentration of the lysin, the optimal temperature is 20 degrees c. the percentage haemolysis-time curves are s-shaped. haemolysis is of the non-osmotic type. ca2+ is not required but inhibits haemolysis in a concentration-dependent fashion, as do mg2+ and zn2+. t ... | 1976 | 8736 |
| genetic variants of human erythrocyte glucose-6-phosphate dehydrogenase. kinetic and thermodynamic parameters of variants a, b, and a- in relation to quaternary structure. | the values of vmax and km for the three genetic variants a, b, and a- of erythrocyte glucose-6-phosphate dehydrogenase have been determined at 10 different ph values in the range from 5.5 to 9.5, and at four different temperatures in the range from 18.5-40.0 degrees. the log vmax versus ph curve for each of the enzymes shows a monotonic increase between ph 5.5 and 7, and a plateau from ph 7.5 upwards. these curves, and their temperature dependence, are compatible with the presence of a single io ... | 1976 | 5448 |
| prolactin-releasing and release-inhibiting factor activities in the bovine, rat, and human pineal gland: in vitro and in vivo studies. | the effects of crude extracts of bovine, rat, and human pineal glands on prolactin (prl) release were studied using an in vitro system. in addition, the effects of a known pineal constituent, arginine vasotocin (avt), and crude bovine pineal extract (bpe) on prl secretion were studied in vivo. normal male rat hemipituitaries (hp), incubated with bpe (13 mg tissue/hp)released 200%, 150%, and 285% more prl into the medium than did their corresponding untreated control halves incubated in either me ... | 1976 | 7446 |
| studies on normal human bone marrow cultures in controlled environment thin-film culture systems. | two thin film culture systems, the controlled environment steady state system (ss) and the rocker tube configuration of that system (rt), were used to identify some of the conditions that appear to maintain morphologic and functional characteristics of cells of human bone marrow explants in vitro. the systems configuration assured continual gassing, control and easy monitoring of the cultures. cytocentrifuge preparations of media of specimens cultured in rt disclosed, though in decreasing number ... | 1976 | 7532 |
| isolation and characterization of a hemagglutinin from amphitrite ornata, a polychaetous annelid. | extracts of the marine polychaetous annelid, amphitrite ornata, agglutinate rat, rabbit, chicken and human erythrocytes and in other work have been shown to inhibit the growth of ehrlich ascites tumors in mice. fractionation of extracts on sephadex g-100 gave three active fractions with molecular weights of 30 000, 54 000 and 100 000. the 30 000 dalton fraction (b) was purified 72-fold by ammonium sulfate precipitation, gel filtration and preparative disc gel electrophoresis. the purified hemagg ... | 1976 | 8117 |
| food protein sources. | work on food, planned by the u.m. (use and management) section of the u.k. committe, was limited to sources of protein because we agreed that more problems calling for research were likely to arise in getting adequate supplies of protein than of other types of food. deer meat can be produced on land too rough and exposed for sheep; parts of the work on their metabolism and food requirements necessitated building a mobile laboratory. the manner in which the nutritive value of maize is affected by ... | 1976 | 8803 |
| human fat cell adenylate cyclase. enzyme characterization and guanine nucleotide effects on epinephrine responsiveness in cell membranes. | human adenylate cyclase (atp pyrophosphate-lyase (cyclizing), ec 4.6.1.1) has been studied in preparations of fat cell membranes ("ghosts"). as reported earlier, under ordinary assay conditions (1.0 mm atp, 5 mm mg2+, 30 degrees c, 10 min incubation) the enzyme was activated 6-fold by epinephrine in the presence of the gtp analog, 5'-guanylyl-imidodiphosphate gmp-p(nh)p (cooper, b. et al. (1975) j. clin. invest. 56, 1350-1353). basal activity was highest during the first 2 min of incubation then ... | 1976 | 8140 |
| human erythrocyte glutathione reductase. i. purification and properties. | 1. glutathione reductase (nad(p)h:oxidized-glutathione oxidoreductase, ec. 1.6.4.2) from human erythrocytes was purified 49 000-fold with an overall yield of 15% and a 280/460 nm absorbance ratio of 6.03. the procedure used was the method of worthington and rosemeyer modified by addition of heating and recrystallization. 2. it was concluded from the results of purification, electrofocusing and inhibition studies that glutathione reductase is a single enzyme which used both nadph and nadh as hydr ... | 1976 | 8143 |
| neutral alpha-mannosidase activity in human serum. | two types of alpha-mannosidase (alpha-d-mannoside mannohydrolase, ec 3.2.1.24) with neutral ph optima exist in serum. the activity with an optimum between ph 6.0 and 6.4 is similar to alpha-mannosidase c, described earlier in tissues. the second activity, with a ph optimum between ph 5.2 and 5.8 is the dominant form in serum. these two forms can be differentiated from each other by gel-filtration, chromatography on deae-cellulose or chromatography on concanavalin-a sepharose. using the chromatog ... | 1976 | 8146 |
| studies on new process procedures in plasma fractionation on an industrial scale. ii. experiments in concentrating dilute albumin solutions using hollow fiber ultrafiltration. | ultrafiltration with a hollow fiber device was studied as an alternative method to the lyophilization or thin-layer distillation techniques in the production of salt-poor, concentrated human albumin preparations for clinical use. a number of process parameters are discussed. combination of gel filtration and ultrafiltration provides an interesting alternative to the currently used systems. | 1976 | 8872 |
| urinary enzyme levels in healthy human subjects and in rats (author's transl). | urinary mdh, ldh, gamma-gt, lap and muramidase levels were studied in 11 healthy persons with a wide age range and in 51 male winstar rats weighing 300 to 400 g. normal ranges of these urinary enzymes were determined and compared with the results of other authors. | 1976 | 8874 |
| reversible transformation of precipitated and nonprecipitated lipoproteins recombined from proteins and lipids of erythrocyte membranes. | the sedimentation at low speed centrifugation of a lipoprotein recombined from the lipids and the strongly bound proteins of the human erythrocyte membrane depends on ph: between 4.5 and 6.0, most of the liproprotein sediments, whereas at ph 7.0-8.5, up to 90% remains in the supernatant. precipitation of the lipoprotein can be reversed by increasing the ph, followed by a brief sonication. the mobility of spin-labelled protein groups in the lipoprotein increases with increasing ph. this mobility ... | 1976 | 8886 |
| comparative results of the express diagnosis of influenza and of virological and immunological studies. | | 1976 | 8904 |
| microplanimetric characteristics of lipidosis in arteriosclerosis of the venous arteries of the human heart. | | 1976 | 8926 |
| serologic properties of strains of cl. perfringens isolated from the intestinal contents of healthy subjects. | a study was made of the quantitative content in the intestine of c1. perfringens strains in 6 healthy persons who stayed in a hermetically sealed space for 1 month and for 1 year. c1. perfingens strains were isolated from the fecal samples of each of the volunteers at various periods of the trial. a total of 570 strains of c1. perfringens of type a with anticellular sera obtained to the strains of various serological groups were studied. serological properties of c1. perfringens strains of type ... | 1976 | 8938 |
| alkaline phosphatase activity in the human tonsils and its relation to tonsillar diseases. | alkaline phosphatase activity was examined in the human tonsils in fetal life and after repeated attacks of acute tonsillitis and in quinsy. gomori's metal precipitate technique was used to demonstrate the phosphatase activity using four different substrates: sodium beta-glycerophosphate and adenosine triphosphate at ph 9, riboflavin 5-phosphate at ph 9.2 and 5-monophosphoric acid at ph 8.3. (2) the phosphatase activity differs somewhat according to the phosphate ester used as a substrate illust ... | 1976 | 8945 |
| purificaiton of neutral human kidney maltase. | | 1976 | 8158 |
| diagnosis of infectious hepatitis by multicomponent analysis with use of field ionization mass spectrometry. | field ionization mass spectrometry has been applied to multicomponent analysis of metabolites in human urine for the diagnosis of metabolic disorders, exemplified here by infectious hepatitis. the molecular weight profiles of carboxylic acids and of "neutral" metabolites in the urine of patients with infectious hepatitis were compared with those in urine of normals. the "neutral" metabolites showed 24 characteristic spectral differences in the range 68 to 215 atomic mass units, which provided co ... | 1976 | 8222 |
| human leukocyte acid hydrolases: characterization of eleven lysosomal enzymes and study of reaction conditions for their automated analysis. | the optimal reaction conditions and kinetic properties of eleven leukocyte acid hydrolases determined with the use of fluorigenic derivatives of 4-methyl-umbelliferone are described. the enzymes studied were acid phosphatase, aryl sulfatase, alpha- and beta-glucosidase, alpha- and beta-galactosidase, alpha-mannosidase, n-acetyl-beta-glucosaminidase, n-acetyl-beta-galactosaminidase, beta-glucuronidase and alpha-fucosidase. more than 90% of the activity of each enzyme was released into a 27,000 x ... | 1976 | 8226 |
| plasma activities of lysosomal enzymes after hepatic dearterialization in man. | six patients with liver metastases from carcinoid or colon carcinoma underwent hepatic derterialization. this operation, known to cause both tumor necrosis and liver cell damage, caused considerable increases of several lysosomal acid hydrolases in the circulation. thus, beta-glucosidase showed a small temporary increase during the operation, followed by a slower but higher reaction reaching a maximum 12 to 36 hours postoperatively. similar reactions were noted for beta-glucuronidase, acid phosp ... | 1976 | 9001 |
| on the morphology of the human sertoli cells under normal conditions and in patients with impaired fertility. | sertoli cells in testicular biopsies from 7 patients with sertoli-cell only syndrome, 8 patients who had been treated with cyclophosphamide for testicular neoplasia, 8 patients with oligozoospermia, 7 patients with cryptorchidism, and 8 patients with seminoma were examined under the electron microscope and compared with sertoli cells of normal tissues. the investigations reveal that each of these conditions of impaired fertility is characterized of a special type of sertoli cell. the cell patter ... | 1976 | 9019 |
| antihypertensive action of propranolol in man: lack of evidence for a neural depressive effect. | the hypothesis that a neural depressive action is related to the antihypertensive effects of beta blockers has been evaluated in 14 essential hypertensive male patients through the circulatory response to noxious stimuli. the pressor reaction to mental arithmetic was primarily mediated by cardiac stimulation (beta receptors activation), that to cold by vasoconstriction (alpha receptors activation). arithmetic and cold were tested to separate the effects of peripheral beta blackade from possible ... | 1976 | 8231 |
| effect of age on oxygen-binding in normal human subjects. | 1. oxygen-binding, plasma and intra-erythrocytic ph, and haemoglobin, 2,3-diphosphoglycerate and inorganic phosphate concentrations were measured in sixty-two healthy non-smokers aged between 18 and 89 years. 2. p50 (oxygen tension at 50% oxygen saturation) expressed at plasma ph 7-40 and pco2 5-33 kpa showed a positive correlation with age. 3. this correlation of p50 with age was closer when p50 was expressed at a constant intra-erythrocytic ph 7-20. on average p50 at intra-erythrocytic ph 7-20 ... | 1976 | 8233 |
| specific gamma-glutamyl transpeptidase inhibitor from human and animal intestines. | an inhibitor of gamma-glutamyl transpeptidase in human and animal intestines was assayed by means of a new method. in mice fed with lsm fodder, accumulation of the inhibitor in the mucous membrane of the small intestine was observed. purified gamma-glutamyl transpeptidase inhibitor from mouse and human intestines was identified as l-serine. neither this amino acid nor purifed inhibitor acted on other intestinal peptidases. presumably, one of the ingredients of lsm feed influences accumulation of ... | 1976 | 9045 |
| structure of bromine containing metabolites of carbromal (author's transl). | isolation of three bromine containing metabolites from human urine is described and their chemical structure is proved by comparison of their physical properties with those of synthetically prepared substances. 2-bromo-2-ethyl-butyramide, a pharmacologically active product, is showed to be the most important bromine containing metabolite by volume. in 3-position hydroxylated metabolites of carbromal, 3-hydroxy-carbromal and 2-bromo-2-ethyl-3-hydroxy-butyramide have same physical properties as th ... | 1976 | 9051 |
| protein chromatography on adsorbents with hydrophobic and ionic groups. chromatography of dodecyl sulphate-solubilized proteins of the human erythrocyte membrane on n-(3-carboxypropionyl)aminodecyl-sepharose. | human erythrocyte 'ghosts' were solubilized in 0.5% (w/v) sodium dodecyl sulphate at ph 4.0(i = 0.012 mol/i). at a loading of 1-2 mg of protein/ml of column volume, all of membrane proteins were adsorbed to a column of cpad n-(3-carboxypropionyl)-aminodecyl-sepharose at ph 4.0 (i = 0-012 mol/1) and room temperature (22 degrees c). many proteins were subsequently desorbed by raising the ph or by including sodium dodecyl sulphate continuously in the eluting buffer. experiments with a series of ads ... | 1976 | 9064 |
| intracellular acid-base heterogeneity in nucleated avian erythrocytes. | 1. intracellular hydrogen ion activity, h+i, was extimated in human erythrocytes and in nucleated avian erythrocytes from measurements of the distribution of ammonia and 5,5'-dimethyloxazolidine-2,4'-dione (dmo) between intracellular and extracellular fluid. 2. in human erythrocytes there was no difference between values for h+i derived from measurements of either dmo or ammonia. 3. in avian erythrocytes, h+i(ammonia) was consistently greater than h+i(dmo), indicating significant acid-base heter ... | 1976 | 8234 |
| the influence of experimental conditions on the efficiency of labeling human serum albumin with 99mtc, using sn(ii) as the reductant. | | 1976 | 8380 |
| species differences in the conjugation of 4-hydroxy-3-methoxyphenylethanol with glucuronic acid and sulphuric acid. | the biosynthesis of the glucuronide and sulphate conjugates of 4-hydroxy-3-methoxyphenylethanol was demonstrated in vitro by using the high-speed supernatant and microsomal fractions of liver respectively. these two conjugates were also produced simultaneously by using the post-mitochondrial fraction of rat, rabbit or guinea-pig liver. in contrast only the glucuronide was synthesized by human liver and only the sulphate by mouse and cat livers. neither of these conjugates was formed by the kidne ... | 1976 | 9078 |
| characterization of the binding of human growth hormone to microsomal membranes from rat liver. | the binding of 125i-labelled human growth hormone to the 100000g microsomal membrane fraction prepared from the livers of normal female rats was dependent on time, temperature, ph, membrane concentration and concentration of 125i-labelled human growth hormone. at 22 degrees c binding reached a steady state after 16h, with the mean maximal specific binding being 20% of the tracer initially added. dissociation of 125i-labelled human growth hormone from the membranes, after addition of excess of un ... | 1976 | 9079 |
| interaction of some neuroleptic and antidepressive agents with human coeruloplasmin. | | 1976 | 9093 |
| stimulation of the peripheral chemoreceptors with sodium bicarbonate. | the i.v. administration of sodium bicarbonate was found to cause an increase in arterial ph, followed by an increase in paco2. this caused a large increase in lung ventilation in in pao2. oxygen administration in human subjects, and anatomical denervation of the chemoreceptors in dogs, caused a substantial delay in the ventilatory responses to sodium bicarbonate. it was concluded that the i.v. administration of sodium bicarbonate provides a method of testing the presence of peripheral chemorefle ... | 1976 | 9124 |
| purification and properties of alkaline ribonuclease from human serum. | 1. five alkaline ribonucleases (ec 3.1.4.22) were purified about 140- to 1900-fold from human serum by phosphocellulose and deae-cellulose chromatographies and sephadex g-75 filtration, with a total recovery of 22%. these were designated as rnaases 1-5. 2. optimum activities were observed at ph 8.5-8.7 for rnaases 1-4, and at ph 7.5 for rnaase 5. the molecular weights of these enzymes were estimated by gel filtration as 45 000, 32 000, 20 000, 13 000 and 8500, respectively. 3. these rnaases were ... | 1976 | 9140 |
| human semen ribonuclease. location, properties and inhibition by sodium dodecyl sulfate, zinc sulfate and edta. | optimal conditions were established for rnase activity measurement. the enzyme was measured in human seminal plasma as well as in spermatozoa. results suggest that sperm enzyme may come from seminal plasma contamination and that rnase may be used as a marker enzyme for seminal plasma contamination. sodium dodecylsulfate, a reagent utilized to produce the solubilization of the spermatozoa, produced a very strong inhibition of the rnase at low concentrations (530 mum). zinc sulfate and edta also p ... | 1976 | 8382 |
| hydrogen-ion titration studies on erythrocyte membranes. | 1. h+ titration was used to detect the presence of ionizable groups on human erythrocyte plasma membranes. between ph2.9 and 11.3, two significant peaks of h+ association/dissociation occur in the differential from of the titration curve, one at ph3. 1. and the other at ph10.3. 2. after disruption of membrane structure by exposure to high ph or by the addition of sodium dodecyl sulphate, maxima of h+ association/dissociation were seen at ph3.1,4.3,6.5,10.3 and 10.7. 3. spectrophotometric assay ... | 1976 | 8038 |
| some biological and physical properties of molluscum contagiosum virus propagated in cell culture. | molluscum contagiosum virus propagated in fl cells of human amnion origin has a one-step growth cycle time of 12 to 14 h. the appearance and exponential increase of intracellular virus preceded the release of extracellular virus by approximately 2 h. demonstration of comparable titers of extracellular and intracellular virus at the end of the replication cycle indicated that a substantial amount of virus remained associated with cells exhibiting cytopathogenic changes. mean buoyant density value ... | 1976 | 8651 |
| human serum and synovial fluid hyaluronidase--bovine testicular hyaluronidase is not a valid substitute in drug evaluation studies. | | 1976 | 8044 |
| extraction of lhrh in human urine: study of the extraction of labelled synthetic hormone. | a method of extraction of synthetic lhrh is studied in human urines, using porous glass (spherosil) and methanol. in the defined conditions the yield is greeter than 80%. it appears that the method is reproducible. the recovery varies essentially with the quantity of spherosil and the ph of methanal. the use of methanol acidified at ph 3 increases the speed and the importance of labelled lhrh recovery. | 1976 | 9184 |
| applications of the extraction and of the radioimmunoassay of lh-rh in human urine. | the existence of endogenous lh rh like immunoreactivity is shown in human urines after appropriate extraction, bu the radioimmunoassay of lh rh. in normaly cycling and menopausal women the quantities of endogenous hormone found in urines are greater after acid extraction than those found after extraction at ph 7. furthermore, the increase observed by extraction in acidified methanol is directly correlated and proportional to the quantity of hormone assayable by extraction at ph 7. the hypothesis ... | 1976 | 9185 |
| oxidation of lactate by human serum. | the oxidation of lactate by lactate dehydrogenase of human serum is described, and the kinetics of the reaction are examined. it seems that at higher ph values which are optimal for the faster moving isoenzymes of lactate dehydrogenase, slower moving entities show only a part of their activity. in contrast, m type isoenzymes are active at lower ph values, where h type entities are partly inactivated. | 1976 | 9208 |
| binding of phosphorylcholine to an igm waldenström as studied by fluorescence spectroscopy and circular dichroism. | the binding characteristics of an igm waldenström(fr) for the ligand phosphorylcholine has been studied by fluorescence spectroscopy. upon phosphorylcholine addition, igm fr exhibited 83% enhancement of the tryptophanyl fluorescence, which was associated with a red shift of the emission maximun (5nm). the same properties were observed with the 7s igm subunits. the association constant ka for phosphorylcholine was 6x10(4) m-1 for igm fr and the 7s subunit, as determined by fluorescence titration, ... | 1976 | 8089 |
| properties of mouse alpha-galactosidase. | alpha-galactosidase has been examined in various murine tissues using the substrate 4-methylumbelliferyl-alpha-galactoside. mouse liver appears to contain a single major form of the enzyme, as judged by chromatography and electrophoresis. the enzmye was purified 467-fold with a yield of about 40% by a method involving chromatography on concanavalin a-sepharose. it has maximal activity at ph 4.2, a km value of 1.4 mm, and energy of activation of 16 400 cal/mol, and a molecular weight of 150 000 a ... | 1976 | 8104 |
| human pancreatic-type ribonucleases with activity against double-stranded ribonucleic acids. | purified acid-thermostable ribonuclease (ribonucleate 3'-pyrimidino-oligonucleotidohydrolase, ec 3.1.4.22) from human pancreas degrades double-stranded rna at 2% the rate for single-stranded rna. the activities against single-stranded rna and double-stranded rna were shown to be due to a single enzyme with properties similar to bovine pancreatic rnaase a. for purposes of comparison the activities against double-stranded rna of crystalline ribonucleases of the whale, rat and cow were assayed and ... | 1976 | 8107 |
| purification and some properties of a neutral protease from human leukocyte granules and its comparison with pancreatic elastase. | 1. a cationic protease has been purified from the granule fraction of blood-donor leukocytes by a preparative method including precipitation by acetone and chromatography on bio-gel a 1.5 m, cm-sephadex c-50 and sephadex g-g-75. 2. the ph optimum against denatured bovine hemoglobin is 7.4. gel chromatography indicated a molecular weight close to 23 000. 3. this neutral protease (ec 3.4.-.-) is able to split the synthetic esters z-ala-nph and acala3ome, its activity on the former substrate being ... | 1976 | 8109 |
| ultramicromethod for the determination of human arginase in the presence of urea. | a technique for arginase determination in body fluids in the presence of urea is described. 14carginine is hydrolysed by arginase to 14curea and ornithine. 14curea is separated with paper chromatography and measured in a liquid scintillation counter. the experimental conditions including the ph, substrate concentration, activator, solvent for chromatography, urea inhibition, and arginase in hemolysates, are discussed. | 1976 | 9217 |
| circulating tissue antigens. iii. identification and characterization of antigens of limited and of wide body is distribution in human gall bladder bile. presence in serum of patients with acute hepatitis. | three antigens shared by bile and tissues (bt-1, bt-2 and bt-3) and one shared by bile and saliva (ba) were identified in human gallbladder bile by immunodiffusion. the former were detected in all bile specimens examined, whereas the latter was detected only in half. bt-1 was limited in distribution to kidney, urine and bile; whereas bt-2 and bt-3 were widely distributed mainly in liver, lung and bile. the antigens were not present in biles of other mammals tested, with the exception of ba which ... | 1976 | 9219 |
| generalization of morphine and lysergic acid diethylamide (lsd) stimulus properties to narcotic analgesics. | the present investigation sought to determine whether the stimulus properties of morphine and lysergic acid diethylamide (lsd) would generalize to several narcotic analgesics which vary in their subjective effects. morphine and saline served as discriminative stimuli for one group of rats in a 2-lever discrimination task. lsd and saline were discriminative stimuli for a second group. depression of one lever in an operant chamber resulted in reinforcement following the administration of morphine ... | 1976 | 8812 |
| glutathione reductase from human erythrocytes. catalytic properties and aggregation. | the catalytic properties of glutathione reductase from human erythrocytes have been studied over a range of buffer conditions and substrate concentrations. this study provides optimal conditions for determining the basic kinetic parameters of the enzyme. the catalytic behaviour of glutathione reductase is consistent with spatially separated binding sites for its substrates. in certain assays anomalies were observed which are correlated with an inactivation of the enzyme by nadph. concurrent sedi ... | 1976 | 9277 |
| the role of interchain disulphide bridges in the conformational stability of human immunoglobulin g1 subclass. hydrogen-deuterium exchange studies. | the hydrogen-deuterium exchange data of human immunoglobulin g1 (igg1) are interpreted by assuming fast fluctuations of the protein conformation, through which the peptide groups become exposed to the solvent. the probability of solvent exposure of peptide hydrogens reflects a rather loose conformation for native igg in comparison with other globular proteins. the probability of solvent exposure is greater than 10(-3) for half of the peptide groups, which shows that the conformational transition ... | 1976 | 9279 |
| appetite in the regulation of food intake for energy (animal and man). | | 1976 | 8817 |
| a micro-method for determination of fatty acid (fiat) and glucose (gliat) incorporation and lipolysis in vitro in needle biopsies of human adipose tissue. | a method for determination of fatty acid (fiat) and glucose (gliat) incorporation into adipose tissue in vitro in needle biopsy specimens of human fat has been developed. 20-150 mg of subcutaneous fat is incubated in an albumin buffer containing a physiological spectrum and concentration of fatty acids and glucose. release of glycerol and fatty acids to the incubation medium and incorporation of labelled palmitic acid and labelled glucose into extracted adipose tissue lipids are determined simul ... | 1976 | 8829 |
| effects of topical bupranolol on intraocular pressure of human subject--a double-blind study-- (author's transl). | | 1976 | 8970 |
| general survey of the present distribution of helminths in iran and comparison with those of past years. | in this paper the literature on helminthiasis in iran is reviewed and the distribution, prevalence and intensity of infections caused by helminths are discussed. a comparison of prevalence and intensity of infections was accomplished in the recent 10 years by the author. hookworm infections are found mostly in the north along the caspian sea, but its prevalence is declining. trichostrongylus are found in iran with a high prevalence in central country. schistosoma haematobium is found in one prov ... | 1976 | 8977 |
| the physiological disposition of etilefrine in man. | pharmacokinetic and metabolic studies with 3h-etilefrine were performed to assess the importance of a first-pass effect on the pharmacodynamic action of this sympathomimetic amine. identical amounts of 3h-activity, ca. 80% of the dose, were excreted in the urine after intravenous or oral administration, which indicates complete enteral absorption of the drug. comparison of the areas under the plasma curves of unchanged etilefrine after both routes of administration resulted in a bioavailability ... | 1975 | 9300 |