| auxotrophic live oral shigella flexneri vaccine protects monkeys against challenge with s. flexneri of different serotypes. | the aromatic-dependent live shigella flexneri y strain sfl114, attenuated by a tn10-inactivated arod gene, was given as an oral vaccine to 14 macaca fascicularis monkeys. a significant clinical attenuation of sfl114 was seen (p = 0.0058) as all vaccinated monkeys tolerated 2 x 10(10)-1 x 10(11) bacteria of sfl114, whereas four out of seven monkeys orally given 1 x 10(11) of the virulent parent strain sfl1 developed shigellosis. the average excretion time for sfl114 and sfl1 were 2 and 18 days, r ... | 1992 | 1557932 |
| [histopathological study on the pars plicata of cynomolgus monkey by the cw nd:yag laser]. | the pars plicata of cynomolgus monkey eyes were coagulated by a continuous wave (cw) nd:yag laser contact system. the intraocular pressure and histopathological changes were observed during the following 3 months. the intraocular pressure decreased during the first week to a minimum level and returned gradually to the pre-coagulation level within 1-3 months. immediately after photocoagulation, both pigment and non-pigmented epithelium of pars plicata were destroyed. three weeks after the coagula ... | 1992 | 1558010 |
| antide bioavailability: single dose administration for suppression of testosterone and inhibin in male monkeys. | the pharmacokinetics and pharmacodynamics of a single sc injection of antide on testosterone (t) and inhibin secretion in intact male cynomolgus monkeys were examined. fifteen primates were randomized to three groups receiving: propylene glycol and water vehicle, 3 mg/kg antide, and 10 mg/kg of antide. antide at the 10 mg/kg dose caused long-term suppression of t ranging from 24-56 days. at the 3 mg/kg dose, suppression of t was of shorter duration. serum antide levels were significantly greater ... | 1992 | 1559337 |
| the effect of topical pgf2 alpha on uveoscleral outflow and outflow facility in the rabbit eye. | prostaglandin f2 alpha (pgf2 alpha) is a powerful ocular hypotensive agent in rabbit, cat, dog, monkey and human. in cynomolgus monkeys, the intraocular pressure (iop) lowering is due to increased uveoscleral outflow (fu). because the anatomy of the rabbit outflow apparatus differs significantly from that of the primate, we sought to determine whether the mechanism of the pgf2 alpha-induced iop fall was the same. pgf2 alpha tromethamine salt (pgf2 alpha-ts) (50 micrograms) applied to one eye of ... | 1992 | 1559555 |
| distribution and biological effects of inhaled 239pu(no3)4 in cynomolgus monkeys. | twenty male cynomolgus monkeys were exposed by inhalation either to an aerosol of 239pu(no3)4 to produce projected initial lung burdens of either 40, 10, or 4 kbq or to a carrier aerosol as a control. animals died or were sacrificed at 0.01, 1, 3, 6, 12, 24, 40, and 99 months after inhalation, and the distribution and biological effects of the 239pu were determined. the 239pu cleared efficiently from the lungs so that less than 0.05 kbq remained at 99 months after exposure to 40 kbq. total skele ... | 1992 | 1561321 |
| hexachlorobenzene (hcb) suppresses circulating progesterone concentrations during the luteal phase in the cynomolgus monkey. | hexachlorobenzene (hcb) is a known reproductive toxin. however, the full spectrum of its reproductive toxicity is unknown. consequently, the effect of hcb on serum oestradiol (e2) and progesterone (p4) concentrations during the follicular (days 1-9), periovulatory (days 10-14) and luteal (days 15 to beginning of next menses) phases was investigated in the spontaneously cycling cynomolgus monkey. adult female cynomolgus monkeys (n = 16) were randomly assigned to one of four treatment groups and o ... | 1992 | 1564247 |
| [the effect of a high-intensity radiation exposure on the brain function of monkeys. the postradiation changes in the eeg response to rhythmic photostimulation]. | in experiments with monkeys (macaca fascicularis) it has been shown that whole-body irradiation with a dose of 45 gy (6.5 gy/s) causes considerable changes in the eeg response to rhythmic photostimulation (ps). these changes are: reduction of the desynchronizing effect of ps with regard to a background rhythmicity; decrease in the reception rate of the rhythms of light flashes (rlf); narrowing of the rlf frequency range; and increase in the reaction momentum. the postirradiation changes in the e ... | 1992 | 1565780 |
| acid reaction products of indole-3-carbinol and their effects on cytochrome p450 and phase ii enzymes in rat and monkey hepatocytes. | the effects of three acid condensation products of indole-3-carbinol (i3c), i.e. 3,3'-diindolylmethane (dim), 5,6,11,12,17,18-hexahydrocyclonona[1,2-b:4,5-b':7,8-b"]tri-indole (cti) and 2,3-bis[3-indolylmethyl]indole (bii), on cytochrome p450 and phase ii enzymes were studied in primary cultures of rat and cynomolgus monkey liver cells. in rat hepatocytes all three indole derivatives dose-relatedly induced the ethoxyresorufin o-dealkylation (erod) activity (to 24-fold) and 7 alpha-hydroxylation ... | 1992 | 1567468 |
| infection of owl monkeys (aotus trivirgatus) and cynomolgus monkeys (macaca fascicularis) with hepatitis e virus from mexico. | owl and cynomolgus monkeys were inoculated with hepatitis e virus (hev) to compare disease models and produce antibody and virus. by immune electron microscopy (iem), all six owl monkeys were shown to have serologic responses manifested by unusually high levels of anti-hev at 6 months, but only three developed hepatitis. virus-related antigen in liver (hev ag) was detected by immunofluorescence microscopy of biopsies from two of four owl monkeys; one with hev ag also had hev in acute-phase bile ... | 1992 | 1569334 |
| malignant lymphoma associated with human aids and with siv-induced immunodeficiency in macaques. | malignant lymphomas associated with human (hiv) and simian (siv) immunodeficiency virus infections are reviewed and compared. recent observation of a high frequency of lymphomas in a series of cynomolgus macaques, highly immunodeficient after infection with sivsm(smm3) are described. in addition to the increased frequency in human and monkey aids, siv and hiv lymphomas share several important features. clinically and by histology they present as aggressive high-grade malignant tumors with a pred ... | 1992 | 1571194 |
| the onset and recovery from airway hyperresponsiveness: relationship with inflammatory cell infiltrates and release of cytotoxic granule proteins. | previous studies from our laboratory have demonstrated a temporal relationship between eosinophil influx into the airways and the onset of airway hyperresponsiveness to inhaled methacholine. the purpose of the present study was to extend this observation by evaluating changes in airway cellular composition and measuring the levels of granulocyte-derived mediators recovered in bal fluid during the onset and recovery from antigen-induced airway hyperresponsiveness. airway cellular composition, air ... | 1992 | 1571822 |
| enzyme immunosorbent assay for ebola virus antigens in tissues of infected primates. | a sandwich enzyme immunosorbent assay (eia) using a mixture of mouse monoclonal antibodies for antigen capture and polyclonal hyperimmune rabbit anti-ebola virus serum for antigen detection was developed and evaluated on the tissues of monkeys naturally or experimentally infected with strains of ebola viruses. when compared with virus isolation, the antigen detection eia was both sensitive and specific: 44 of 45 (97.7%) liver homogenates and 38 of 41 (92.7%) spleen homogenates that were culture ... | 1992 | 1572982 |
| activity in monkey substantia nigra neurons related to a simple learned movement. | single cell activity was recorded in the pars compacta (snc) and pars reticulata (snr) of the substantia nigra (sn) in 4 unanesthetized macaca fascicularis to determine the motor role of the nucleus. animals were trained to perform a simple task that involved moving a lever by elbow flexion-extensions, in the horizontal plane using the hand contralateral to the recording site. two monkeys learnt to execute the task on both sides. electromyograms (emg) of limb muscles were recorded simultaneously ... | 1992 | 1577102 |
| immunoglobulin ch gene family in hominoids and its evolutionary history. | the organization of the human immunoglobulin ch gene suggests that a gene duplication involving the c gamma-c gamma-c epsilon-c alpha region has occurred during evolution. we previously showed that both chimpanzee and gorilla have two 5'-c epsilon-c alpha-3', as in human, and that orangutan, gibbon, and old world monkeys have one c epsilon gene and one, two, and one c alpha gene(s), respectively. in addition to these clustered ch genes, there is one processed c epsilon pseudogene in each species ... | 1992 | 1577482 |
| localization of corticotropin-releasing factor-like immunoreactivity in monkey olfactory bulb and secondary olfactory areas. | electrophysiological and anatomical observations suggest that terminals of olfactory bulb mitral cells ending in rat primary olfactory cortex exert certain postsynaptic effects via an excitatory amino acid neurotransmitter. recent anatomical studies have shown that several peptides, most notably corticotropin-releasing factor (crf) (imaki et al., '89) brain res., 496: 35-44), are also localized within rat olfactory bulb projection neurons, thus raising the possibility that there is a peptide cot ... | 1992 | 1577989 |
| functional properties of single neurons in the face primary motor cortex of the primate. i. input and output features of tongue motor cortex. | 1. we have recently demonstrated that reversible, cooling-induced inactivation of the face motor cortex results in a severe impairment in the ability of monkeys (macaca fascicularis) to perform a tongue-protrusion task but produces only relatively minor effects on the performance of a biting task by the same monkeys. to establish a neuronal correlate for these different behavioral relations, the present study has detailed the afferent input and intracortical microstimulation (icms)-defined outpu ... | 1992 | 1578252 |
| primary afferent interactions: analysis of calcitonin gene-related peptide-immunoreactive terminals in contact with unlabeled and gaba-immunoreactive profiles in the monkey dorsal horn. | the present study analyses the relationship of calcitonin gene-related peptide (cgrp)-immunoreactive primary afferent terminals with unlabeled and gaba-immunoreactive profiles in the primate (macaca fascicularis) dorsal horn. one-hundred cgrp-immunoreactive terminals located in the superficial dorsal horn were quantitatively analysed and all profiles in apposition or in synaptic contact with these terminals were categorized as either axon terminals or dendrites with or without vesicles. these pr ... | 1992 | 1579216 |
| the use of thalamotomy in the treatment of levodopa-induced dyskinesia. | peak dose dyskinesia is a major problem in the treatment of parkinsonian patients with levodopa and yet this remains the best pharmacological agent for treating the condition. the hypothesis which this research set out to test was that thalamotomy in the area of the thalamus which receives the input from the medial segment of the globus pallidus would decrease or prevent the dyskinesia. a well established primate model of parkinsonism was used. eight monkeys (macaca fascicularis) were rendered p ... | 1992 | 1580197 |
| effects of topical ethacrynic acid adducts on intraocular pressure in rabbits and monkeys. | we evaluated the effect of topical ethacrynic acid on rabbit and monkey intraocular pressure. in a preliminary experiment, 100-mmol/l ethacrynic acid applied topically to dutch-belted rabbit eyes was associated with an 8-mm hg lowering of intraocular pressure. however, corneal edema was severe, and the corneal epithelium sloughed off. to try to maintain the pressure-lowering effect but reduce the corneal side effects, we attempted to create an adduct of ethacrynic acid by utilizing ethacrynic ac ... | 1992 | 1580849 |
| polymyxin b-induced bronchial neutrophilia does not alter airway responsiveness to methacholine in cynomolgus monkeys. | the purpose of this study was to develop a primate model of chronic bronchial neutrophilia to investigate the role of neutrophils in the pathogenesis of airway hyperresponsiveness. ten adult male cynomolgus monkeys (macaca fascicularis) were anaesthetized and intubated for each study. six animals each received a total of seven inhalation treatments with polymyxin b (200 micrograms) over a 24 day period. four control animals received an identical treatment regime with vehicle inhalations. airway ... | 1992 | 1586875 |
| c-terminal modifications of nonpeptide renin inhibitors: improved oral bioavailability via modification of physicochemical properties. | we describe the development of a series of soluble, potent, and bioavailable nonpeptide renin inhibitors. these inhibitors derived from a series of novel nonpeptide renin inhibitors which were recently identified in our laboratories, by alteration of the nature of the c-terminus (p2') of the molecules. introduction of basic substituents into modified hydroxyethylene dipeptide isosteres gave inhibitors with improved solubility as well as improved potency against human plasma renin. in addition, t ... | 1992 | 1588555 |
| hepatitis e virus (hev): strain variation in the nonstructural gene region encoding consensus motifs for an rna-dependent rna polymerase and an atp/gtp binding site. | hepatitis is transmitted by a number of infectious agents. the epidemiological characterization of waterborne or enterically transmitted non-a, non-b hepatitis (et-nanbh) is unique when compared with other known hepatitides. we have reported on the molecular cloning of a cdna clone derived from the etiologic agent associated with et-nanbh, the hepatitis e virus (hev). the complete sequence of these first molecular clones, isolated from an hev-infected human after passage in macaca fascicularis ( ... | 1992 | 1589964 |
| regional differences in arterial low density lipoprotein metabolism in surgically postmenopausal cynomolgus monkeys. effects of estrogen and progesterone replacement therapy. | to determine if arterial lipoprotein metabolism may be involved in mediating well-known anatomic regional differences in susceptibility to atherosclerosis, arterial low density lipoprotein (ldl) metabolism and extent of atherosclerosis were studied in 17 ovariectomized female cynomolgus monkeys. the animals were fed an atherogenic diet for 18 weeks, during which time one group received 17 beta-estradiol and cyclic progesterone treatment (n = 9) and the controls received no hormone replacement th ... | 1992 | 1591231 |
| the role of cholesteryl ester transfer protein in primate apolipoprotein a-i metabolism. insights from studies with transgenic mice. | to assess the effects of cholesteryl ester transfer protein (cetp) on the primate lipoprotein profile, a transgenic mouse expressing cynomolgus monkey cetp was developed. the c57bl/6 mouse was used, and four lines expressing the primate cetp were established. the level of cetp activity in the plasma of the transgenic mice ranged from values similar to those obtained for the monkey to levels approximately sixfold higher than that in the normal monkey. when all of the lines were taken into conside ... | 1992 | 1591233 |
| reproductive toxicity of chronic lead exposure in the female cynomolgus monkey. | the effect of chronic lead (pb) exposure on menstrual function and circulating concentrations of luteinizing hormone (lh), follicle stimulating hormone (fsh), estradiol (e2) and progesterone (p4) were studied in female nulliparous cynomolgus monkeys (n = 32). pb acetate (1500 micrograms/kg bw/day) was administered by capsule to monkeys in teh following groups; exposure from birth to 10 years (lifetime, n = 8), postnatal day 300 to 10 years (adolescent, n = 8), and postnatal days 0 to 400 (childh ... | 1992 | 1591470 |
| antitumor effects of b3-pe and b3-lyspe40 in a nude mouse model of human breast cancer and the evaluation of b3-pe toxicity in monkeys. | b3 is a tumor-reactive monoclonal antibody (mab) that binds to a limited number of normal tissues. immunotoxins made with b3 coupled to either pseudomonas exotoxin (pe) or recombinant forms of pe with a deletion of the cell-binding domain (lyspe40) have been shown to cause complete tumor regression in nude mice bearing a rapidly growing a431 (l. h. pai et al., proc. natl. acad. sci. usa, 88: 3358-3362, 1991) human epidermoid carcinoma. in this study we show that an immunotoxin composed of mab b3 ... | 1992 | 1591729 |
| fibrinous reaction on implanted intraocular lenses. a comparison of conventional pmma and heparin surface modified lenses. | we studied the fibrinous reaction after intraocular lens (iol) implantation in the posterior chamber of cynomolgus monkeys. in 50% of the eyes, we implanted an iol made of conventional poly-(methyl methacrylate) (pmma); in the remaining eyes we implanted a pmma iol with a heparin modified surface. two, 4, 8, and 18 weeks after surgery the eyes were examined by slitlamp for fibrinous reactions on and around the iol surface. at weeks 4, 8, and 18 there was a marked decrease in fibrinous reaction i ... | 1992 | 1593429 |
| a comparison of total body water measurements using whole-body magnetic resonance imaging versus tritium dilution in primates. | standard techniques conventionally used to assess body composition have various drawbacks which include a requirement for specialized equipment and expertise not widely available, radiation exposure, extensive study time periods, and limited clinical utility. anthropometric methods, which are more clinically feasible, also involve substantially greater calculation error. this study was designed to compare a newly developed whole-body magnetic resonance (mr) imaging technique with standard tritiu ... | 1992 | 1593876 |
| taste neurons in the cortex of the alert cynomolgus monkey. | the activity of single neurons in the gustatory cortex of alert cynomolgus monkeys was analyzed. taste-evoked activity in response to the four prototypical taste stimuli was recorded from a cortical gustatory area comprising the frontal operculum and adjoining anterior insula. spontaneous activity for 364 gustatory neurons was 3.9 +/- 4.9 (mean +/- sd) spikes/s. mean net (gross minus spontaneous) discharge rates for all gustatory neurons were: 1.0 m glucose = 4.9 +/- 11.6, 0.3 m nacl = 3.2 +/- 7 ... | 1992 | 1596754 |
| peripheral and not central suppression of ovarian function during osmotic pump infusion of adrenocorticotropin-(1-24) for one menstrual cycle in the cynomolgus monkey and its partial compensation by a transitory elevation of sex hormone-binding globulin levels. | the purpose of our study was to assess the impact of subchronic administration of acth-(1-24) on ovarian function in the primate using a repeated measures experimental design. osmotic pumps that released acth-(1-24) at a dose of 67 micrograms/day were implanted sc in four cynomolgus monkeys for one menstrual cycle. the pumps were filled with saline for the two control cycles, one of which preceded and one of which followed peptide infusion. administration of acth-(1-24) elevated cortisol levels ... | 1992 | 1597155 |
| the source of sensory fibres of the inferior conjunctiva of monkeys. | dissection, light and electron microscopy combined with degeneration induced by intracranial nerve lesions and superficial nerve transection were used to trace the sources of sensory fibres of the inferior conjunctiva of cynomolgus monkeys. most nerves destined for the inferior conjunctiva were branches of the lacrimal nerve and entered the inferior eyelid laterally. following unilateral ophthalmic neurotomy in three monkeys and combined ophthalmic/maxillary neurotomy in one monkey, substantial ... | 1992 | 1597293 |
| association of ebola-related reston virus particles and antigen with tissue lesions of monkeys imported to the united states. | during 1989-1990, an epizootic involving a filovirus closely related to ebola virus occurred in a reston, virginia, primate-holding facility. tissues were collected from cynomolgus monkeys and examined by electron microscopy and immunohistochemistry for ebola-related viral antigen. viral replication was extensive in fixed tissue macrophages, interstitial fibroblasts of many organs, circulating macrophages and monocytes, and was observed less frequently in vascular endothelial cells, hepatocytes, ... | 1992 | 1597531 |
| stabilization of gaze during circular locomotion in light. i. compensatory head and eye nystagmus in the running monkey. | 1. a rhesus and cynomolgus monkey were trained to run around the perimeter of a circular platform in light. we call this "circular locomotion" because forward motion had an angular component. head and body velocity in space were recorded with angular rate sensors and eye movements with electrooculography (eog). from these measurements we derived signals related to the angular velocity of the eyes in the head (eh), of the head on the body (hb), of gaze on the body (gb), of the body in space (bs), ... | 1992 | 1597704 |
| susceptibility of macaca fascicularis monkeys from mauritius to different species of plasmodium. | macaca fascicularis monkeys from mauritius were shown to be susceptible via sporozoite inoculation to 7 species of plasmodium (p. fragile, p. coatneyi, p. gonderi, p. inui, p. cynomolgi, p. knowlesi, and p. fieldi), indigenous to macaques in southeastern asia. four monkeys were sequentially infected with different species of plasmodium to determine maximum and course of parasitemia. in 2 nonsplenectomized monkeys, p. fragile developed maximum parasite counts of only 134 and 155/microliters. for ... | 1992 | 1597796 |
| [the effect of ethanol on the metabolism of methamphetamine]. | in order to clarify the effects of ethanol (etoh) on metabolism of methamphetamine hydrochloride (ma-hcl), the plasma and urine samples were taken from three groups of three male crab-eating monkeys (macaca fascicularis). ma-hcl was given to the first group, both ma-hcl and etoh were given once to the second group, the after consecutive administration of etoh for four weeks, both ma-hcl and etoh were given to the third group. comparisons were made of the sequential change in the concentration of ... | 1992 | 1597931 |
| live oral auxotrophic shigella flexneri sfl124 vaccine with a deleted arod gene: characterization and monkey protection studies. | shigella flexneri sfl124, with a deletion encompassing all, or nearly all, of the coding sequence of gene arod was obtained after selection on a fusaric acid medium supplemented with 2,3-dihydroxybenzoic acid for tetracycline-sensitive mutants of s. flexneri sfl114 which is an arod::tn10 transductant. two of 20 tetracycline-sensitive mutants tested in colony hybridization with a 32p-labelled dna probe of approximately 1400 base pairs (comprising all except the 75 n-terminal base pairs of the cod ... | 1992 | 1598787 |
| effects of a low-fat diet on brain serotonergic responsivity in cynomolgus monkeys. | | 1992 | 1599991 |
| in vivo distribution of [11c]-busulfan in cynomolgus monkey and in the brain of a human patient. | the in vivo distribution of the antileukemic agent busulfan labeled with the positron-emitting radionuclide carbon 11 was investigated in cynomolgus monkeys and in a human patient using positron emission tomography. after i.v. injection of the radiotracer, its regional uptake was monitored for about 1 h in the monkey's body and, in a separate experiment, in the monkey's brain. the concentration of radioactivity in the liver, which showed the highest levels of all the organs scanned, increased th ... | 1992 | 1600599 |
| high-performance liquid chromatographic procedure for specificity testing of radioimmunoassays: rolipram. | a high-performance liquid chromatographic (hplc) procedure for testing the specificity of radioimmunoassays (ria) was developed using the same method of extraction as in the ria, followed by fractionation of the extract by hplc and subsequent measurement of cross-reactions in all the fractions according to the normal ria procedure. the ria of rolipram, an antidepressant drug, was checked in plasma samples obtained from pharmacokinetic studies in rats, rabbits, cynomolgus monkeys and humans. the ... | 1992 | 1601964 |
| establishment and characterization of macaca fascicularis lymphoblastoid cell lines. | a panel of cynomolgus macaque lymphoblastoid cell lines (lcl) was established by transforming peripheral blood mononuclear cells (pbmc) with herpesvirus papio (hvp), and selected lines were examined by flow cytometry. results indicate that hvp-transformed macaque lcl are phenotypically heterogeneous and resemble human epstein-barr virus (ebv)-transformed lcl in the abundant expression of major histocompatibility complex (mhc) class i and class ii molecules. at least some lines are of b cell orig ... | 1992 | 1602455 |
| a prospective analysis of endometrial cycle changes by ultrasound in the female cynomolgus monkey. | endometrial cycle changes in adule female cynomolgus monkeys with normal ovulatory cycles were examined prospectively by real-time ultrasound. endometrial thickness, as measured by ultrasound, was correlated with cycle day and serum estradiol (e2) and progesterone (p4) levels. we conclude that ultrasound is a reliable method of diagnosis of endometrial cycle stage. | 1992 | 1602457 |
| neuroactive amino acids in the area postrema. an immunocytochemical investigation in rat with some observations in cat and monkey (macaca fascicularis). | the localization of five neuroactive amino acids in the rat area postrema was studied by postembedding immunocytochemistry in semithin and ultrathin sections. antisera to gaba, glycine, glutamate and aspartate produced labelling of cells that were identified as neurons in the electron microscope. gaba-like and glycine-like immunoreactivities occurred in about 20% and 60% of the neurons, respectively, and a minor proportion of the cells displayed both immunoreactivities, suggesting a cellular col ... | 1992 | 1605365 |
| localization of a carbohydrate antigen associated with growing oocytes and ovarian surface epithelium. | we used a monoclonal antibody (ps1) to a carbohydrate antigen to study the development of the oocyte and follicle during early stages of differentiation in several mammalian species. this antigen has been shown to localize within the cytoplasm of oocytes in primordial follicles as well as in growing oocytes. it is also localized within distinct layers of the zona pellucida (zp) of developing follicles. although this antibody was made against a specific zp glycoprotein, the antigen also appears t ... | 1992 | 1607636 |
| developmental changes in the uptake of testosterone by the primate brain. | during the neonatal period in male macaques, the testis produces adult-like levels of plasma testosterone (t), but the function of this in development is not understood. to investigate the interaction of t with the neonatal brain, 4 male and 5 female cynomolgus monkeys were gonadectomized 2-5 days after birth, and were injected subcutaneously 3 days later with 500 microci [3h]-testosterone ([3h]-t). 60 min later, brains and other tissue samples were removed. purified nuclear pellets were prepare ... | 1992 | 1608511 |
| metabolism of [14c]benzene by cynomolgus monkeys and chimpanzees. | rodent bioassays indicate that b6c3f1 mice are more sensitive to the carcinogenicity of benzene than are rats. the urinary profile of benzene metabolites is different in rats vs mice. mice produce higher proportions of hydroquinone conjugates and muconic acid, indicators of metabolism via pathways leading to putative toxic metabolites, than do rats. in both species, metabolism to hydroquinone and muconic acid is favored at low concentrations of benzene, indicating that these pathways are easily ... | 1992 | 1609420 |
| neurovirulence tests of type 3 oral poliovirus vaccine manufactured by lederle laboratories, 1964-1988. | oral poliovirus vaccine (opv) is tested for safety by evaluation of neurovirulence in rhesus and cynomolgus macaques. after intraspinal or intrathalamic injection of varying doses of vaccine, monkeys are followed for 17-21 days, killed, and a histopathological evaluation is made of the severity of poliomyelitis lesions in the spinal cord and brainstem. each production lot of vaccine is compared with a type 1 opv reference virus tested by the same method. records of neurovirulence tests on produc ... | 1992 | 1609550 |
| nucleotide sequence of the cynomolgus monkey apolipoprotein a-i gene and corresponding flanking regions. | the cynomolgus monkey (macaca fascicularis) apolipoprotein a-i (apo a-i) gene and corresponding flanking regions have been isolated from a cynomolgus monkey genomic library and completely sequenced. comparison with the human sequence shows a greater than 90% homology with the monkey sequence, overall. the monkey apo a-i structural gene (1856 bp) is representative of the general apolipoprotein gene structure and consists of four exons and three introns. minor differences exist between the monkey ... | 1992 | 1610902 |
| transplantation of embryonic serotonin immunoreactive neurons into the transected spinal cord of adult monkey (macaca fascicularis). | five adult monkeys (macaca fascicularis) underwent a total section of the spinal cord at the thoracic level (t6). four of them received a daily treatment with cyclosporin (10 mg/kg). ten days later, two animals treated with cyclosporin and one without cyclosporin received at t8 and t10 levels an injection of a cell suspension prepared from the rhombencephalon of a 40-day-old macaque embryo. two control animals received one injection of hank's balanced salt solution. the animals were sacrificed a ... | 1992 | 1611532 |
| exogenous gonadotrophin-releasing hormone (gnrh) stimulates lh secretion in male monkeys (macaca fascicularis) treated chronically with high doses of a gnrh antagonist. | we reported previously that after a single injection of a gonadotrophin-releasing hormone (gnrh) antagonist to male monkeys, exogenous gnrh stimulated lh secretion in a time- and dose-dependent manner, indicating that gnrh antagonist-induced blockade of lh secretion resulted from pituitary gnrh receptor occupancy. the present study was performed to investigate whether gnrh can also restore a blockade of lh and testosterone secretion during chronic gnrh antagonist administration. four adult male ... | 1992 | 1613445 |
| infection of cynomolgus monkeys with a chimeric hiv-1/sivmac virus that expresses the hiv-1 envelope glycoproteins. | replication competent chimeric viruses that express the gag and pol proteins of sivmac and the env proteins of hiv-1 were made. one such chimeric virus, shiv-4, that expresses the vif, vpx, vpr, and nef regulatory genes of siv and the tat and rev regulatory genes of hiv-1 replicated efficiently in cynomolgus monkeys. this model system can be used to evaluate the efficacy of anti-hiv-1 vaccines directed at the envelope glycoproteins, anti-hiv-1 envelope glycoprotein antiserum or monoclonal antibo ... | 1992 | 1613662 |
| postpartum lactational anovulation in a nonhuman primate (macaca fascicularis): endogenous opiate mediation of suckling-induced hyperprolactinemia. | the physiological amenorrhea occurring in suckled females has been associated with both hypopulsatile gonadotropin secretion and hyperprolactinemia. to test whether these phenomena are opiate mediated and whether these effects are dependent on the presence of ovaries, we studied six suckled, lactating cynomolgus monkeys, three with intact ovaries and three that were ovariectomized 14 days postpartum. frequent blood sampling (every 15 min) was performed at approximately monthly intervals using ch ... | 1992 | 1619032 |
| outbreak of fatal illness among captive macaques in the philippines caused by an ebola-related filovirus. | following the detection of an ebola-like virus in cynomolgus macaques recently imported into the united states from the philippines, studies were initiated to document transmission at export facilities located in the latter country. at one export facility, 52.8% of 161 monkeys that died over a 2.5-month period were shown to be infected with this virus using an enzyme-linked immunosorbent assay to detect antigen in liver homogenates. a case fatality rate of 82.4% was documented for the infected m ... | 1992 | 1621890 |
| acute oral toxicity of the new quinolone antibacterial agent levofloxacin in mice, rats and monkeys. | acute oral toxicity of (-)-(s)-9-fluoro-2,3-dihydro-3-methyl-10-(4- methyl-1-piperazinyl)-7-oxo-7h-pyrido [1,2,3-de][1,4]benzoxazine-6-carboxylic acid hemihydrate (levofloxacin, dr-3355, cas 100986-85-4), a new quinolone antibacterial agent, was studied in ddy mice, sd rats and cynomolgus monkeys. ld50 values were 1,881 mg/kg for males and 1,803 mg/kg for females in mice, 1,478 mg/kg for males and 1,507 mg/kg for females in rats and more than 250 mg/kg in females monkeys. toxic signs included th ... | 1992 | 1622433 |
| twenty-six-week oral toxicity of the new quinolone antibacterial agent levofloxacin in rats and cynomolgus monkeys. | the oral 26-week toxicity of (-)-(s)-9-fluoro-2,3-dihydro-3-methyl-10-(4- methyl-1-piperazinyl)-7-oxo-7h-pyrido [1,2,3-de][1,4]benzoxazine-6-carboxylic acid hemihydrate (levofloxacin, dr-3355, cas 100986-85-4) was investigated in rats and monkeys. rats receiving higher doses of dr-3355 exhibited an increased number of larger fecal pellets, salivation, lower neutrophil counts, enlargement of the cecum and prominent goblet cells in the cecal mucosa. monkeys did not show any changes due to dr-3355 ... | 1992 | 1622434 |
| contractile cells in the human scleral spur. | the scleral spur in 37 human (age 17-87 years) and six cynomolgus monkey eyes (2-4 years) was investigated. serial meridional and tangential sections were studied with ultrastructural and immunocytochemical methods. the bundles of the ciliary muscle do not enter the scleral spur, but their tendons, which consist of elastic fibres join the elastic fibres in the scleral spur. within the scleral spur a population of circularly oriented and spindle-shaped cells is found. in contrast to the ciliary m ... | 1992 | 1623940 |
| the effect of 12(r)-hydroxyeicosatetraenoic acid on aqueous humor dynamics in the rabbit and cynomolgus monkey. | | 1992 | 1623968 |
| development of dental plaque on the incisor teeth of monkeys (macaca fascicularis). | bacterial plaque which had accumulated for 0.5, 1, 2, 4, 7, 14 and 28 days was cultured from the labial surface of one of the central incisor teeth in 15 monkeys (macaca fascicularis). each sampling area was defined by a metal well contained within a custom-made removable acrylic block. the number (log10) of the total colony forming units (c.f.u.) increased significantly from 3.40 to 6.02 between 0.5 and 28 days. similarly the number of streptococci increased from 2.80 to 4.79 during the same pe ... | 1992 | 1624618 |
| clinical and light microscopic observations of gingivitis and early ligature-induced periodontitis in the cynomolgus monkey. | clinical and histological observations were made on gingivitis and ligature-induced periodontitis in 4 adult female cynomolgus monkeys (macaca fascicularis) to define the changes occurring in the early periodontitis lesion. silk ligatures were tied around selected posterior teeth and replaced weekly for 4 weeks. the changes from gingivitis to periodontitis induced by ligation, and back to a state of clinical health after ligature removal, scaling and polishing, were characterized by plaque index ... | 1992 | 1625153 |
| distribution of enkephalin and its relation to serotonin in cat and monkey spinal cord and brain stem. | the distribution of enkephalin (enk)-like immunoreactivity (li) in spinal cord and medulla oblongata of cat and gray monkey (macaca fascicularis) was studied by use of immunofluorescence and peroxidase antiperoxidase (pap) techniques. possible coexistence between enk- and 5-hydroxytryptamine (5-ht)-li was also analyzed with double labeling immunofluorescence. furthermore, in situ hybridization was used to demonstrate cell bodies in the brain stem expressing mrna encoding for enk. enk-immunoreact ... | 1992 | 1626315 |
| the mechanism of suckling in two species of infant mammal: miniature pigs and long-tailed macaques. | suckling is the form of feeding unique to infant mammals. the mechanism used by infant mammals to withdraw liquid from the nipple is the subject of considerable debate. suckling has been examined in two species of infant mammals: miniature pigs and long-tailed macaques. in both species radio-opaque markers were inserted into the tongue and jaws; the movements of the jaw and tongue (and also of specific regions within the tongue) plus the movement of milk containing barium were studied by high-sp ... | 1992 | 1629663 |
| gustatory neural coding in the monkey cortex: l-amino acids. | 1. single-neuron activity in the primary gustatory cortex of the alert cynomolgus monkey (macaca fascicularis) was analyzed in response to a range of taste stimuli. tastants included the four prototypical stimuli (glucose, nacl, hcl, and quinine), fruit juice, and 12 amino acids selected for their chemical characteristics, nutritional significance, and biological importance, as well as for the availability of human psychophysical data on their perceived qualities. 2. taste-evoked responses could ... | 1992 | 1629764 |
| exposure to methyl mercury from birth to adulthood impairs high-frequency hearing in monkeys. | hearing deficits are a frequent consequence of both developmental and adult methyl mercury exposure in humans. however, a detailed characterization of these deficits has not been performed in either humans or animals. cynomolgus monkeys (macaca fascicularis) were dosed from birth to 7 years of age with 50 micrograms/kg/day of mercury as methyl mercuric chloride. steady-state blood mercury levels during dosing were 0.6-0.9 ppm. when monkeys were 14 years old, pure tone detection thresholds were d ... | 1992 | 1631895 |
| effects of epidermal growth factor on apo b mrna levels and apo b accumulation in the media of primate hepatocytes in culture. | egf has been shown to augment albumin and apolipoprotein a-i secretion by cynomolgus monkey hepatocytes in primary culture without stimulating cell division. this study was undertaken to determine what effect egf had on apo b secretion by those hepatocytes. the results indicate that egf (3 nm final concentration) severely inhibits the rate at which apo b accumulates in the culture medium of primate hepatocytes. that effect was evident within 48 hours of treatment, and by 72 hours the rate that a ... | 1992 | 1632766 |
| short-term administration of estrogen and vascular responses of atherosclerotic coronary arteries. | this experiment sought to determine the effect of short-term administration of estrogen on endothelium-dependent dilation in the coronary arteries of 13 surgically postmenopausal female cynomolgus monkeys. | 1992 | 1634685 |
| molecular mimicry: uveitis induced in macaca fascicularis by microbial protein having sequence homology with retinal s-antigen. | s-antigen (s-ag), a well characterized 45-kda protein in the photoreceptor cells, induces predominantly t-cell-mediated autoimmune uveitis when injected into experimental animals. recently, we have shown that native histone h3 protein derived from yeast (saccharomyces cerevisiae), or a synthetic peptide that is homologous with s-ag peptide m in having six consecutive amino acids, induces experimental autoimmune uveitis (eau) similar to that induced by native s-ag in the lewis rat. in this study, ... | 1992 | 1635290 |
| plasma levels of eosinophil granule major basic protein in pregnant cynomolgus monkeys. | the sera of all pregnant women contain increased amounts of a protein biochemically and immunologically similar to the eosinophil granule major basic protein (mbp). immunofluorescence shows that the pregnancy-associated mbp is localized to placental trophoblastic cells. this information raises important questions about the function of pregnancy-associated mbp because of the potential biological functions attributed to its eosinophil counterpart (namely, its potent toxic and cytostimulatory activ ... | 1992 | 1635912 |
| biosynthesis of prostaglandins from 17(18)epoxy-eicosatetraenoic acid, a cytochrome p-450 metabolite of eicosapentaenoic acid. | eicosapentaenoic acid (20:5(n - 3)) is oxygenated to 17s(18r)epoxyeicosatetraenoic acid (epete) by microsomes of monkey seminal vesicles, which also are rich in prostaglandin (pg) h synthase. the metabolism of racemic [14c]17(18)epete by pgh synthase of sheep vesicular glands was investigated in the present report. the two main metabolites were identified by gc-ms as 17(18)epoxyprostagland e2 (17(18)eppge2) and 17(18)eppgf2 alpha. the structures were confirmed by chemical synthesis of these pros ... | 1992 | 1637854 |
| different susceptibilities to attenuated poliovirus of cynomolgus monkeys from the philippines and other southeast asian countries. | susceptibilities to attenuated poliovirus of cynomolgus monkeys were assessed by the intraspinal inoculation method with the same single bulk lot as the reference for each of the three poliovirus types. the lesion-inducing virus dose in the spinal cord of 50% of monkeys inoculated (lid50) varied widely from 10(6.4) to lower than 10(2.5), the difference being as much as 10,000-fold, depending on the birth place of the monkey. the birth places of the monkeys used were traced and categorized into t ... | 1992 | 1640648 |
| tumor induction in monkeys after administration of dimethylhydrazine. | 1,2-dimethylhydrazine (dmh) was administered subcutaneously to nine macaca fascicularis monkeys (6 males and 3 females) at doses of 16 mg/kg body weight, three times a month for two years. colon cancer was detected in two male monkeys after total dmh doses of 1080 and 3696 mg (528 and 400 mg/kg body wt., respectively). a uterine tumor was induced in one female monkey which received 3648 mg of dmh (608 mg/kg body wt.). latent periods of tumor development were 34, 47 and 55 weeks, respectively. hi ... | 1992 | 1644662 |
| solid-phase enzyme-linked immunosorbent assay for hepatitis e virus igg and igm antibodies utilizing recombinant antigens and synthetic peptides. | four recombinant antigens representing two distinct antigenic domains from two different strains of hepatitis e virus (hev), were used individually to develop four elisas designed to detect antibodies to hev. both igg and igm class antibodies to hev were detected in 7 of 8 pedigreed serum/plasma from known outbreaks of hev in mexico, burma, somalia and pakistan. in addition, specific hev-antibodies were detected in cynomolgus macaques following inoculation with various hev strains. anti-hev was ... | 1992 | 1644893 |
| synthesis of carbon-11 labelled sch 39166, a new selective dopamine d-1 receptor ligand, and preliminary pet investigations. | sch 39166 [(-)-trans-6,7,7a,8,9, 13b-hexahydro-3-chloro-2-hydroxy-n-methyl-5h-benzo(d)naphtho-(2,1- b)azepine] is a new more selective dopamine d-1 receptor antagonist than the widely used sch 23390. [11c]sch 39166 was prepared by n-methylation of the desmethyl compound sch 40853 [(-)-trans-6,7,7a,8,9,13b-hexahydro-3-chloro-2-hydroxy-5h- benzo(d)naphtho-(2,1-b)azepine] with [11c]methyl iodide. reaction in acetone with subsequent straight-phase semi-preparative hplc resulted in 20-30% radiochemic ... | 1991 | 1646191 |
| presence of antibody to cyno-ebv in domestically bred cynomolgus monkeys (macaca fascicularis). | the presence of an antibody to the cyno-ebv was studied with cyno-ebv bearing cell line (ts-b-9) as antigen in 186 domestically bred cynomolgus monkeys (macaca fascicularis) ranging in age from birth to 18 yr as well as in 20 adult (older than 10 yr) cynomolgus monkeys of wild origin. the maternal antibody to cyno-ebv persisted for less than four mo after birth in infant monkeys. after one yr of age, all the domestically bred monkeys were consistently positive for cyno-ebv. in the monkeys of wil ... | 1991 | 1646886 |
| antigen-induced mediator release in primates. | we examined the release of bronchoactive mediators into the airways of allergic primates during the acute response to specific antigen inhalation. twelve adult male cynomolgus monkeys (macaca fascicularis) with a naturally occurring respiratory sensitivity to inhaled ascaris suum extract were anesthetized and intubated for each study. respiratory system resistance (rrs) and dynamic lung compliance (cldyn) were measured before and after antigen inhalation, and the release of mediators into the ai ... | 1991 | 1648317 |
| sequence analysis of a new hepatitis a virus naturally infecting cynomolgus macaques (macaca fascicularis). | a new isolate of hepatitis a virus (hav), cy-145, was isolated from stool specimens obtained from cynomolgus macaques naturally infected with this agent. sequence analysis of the capsid region of the genome indicated that this virus differed from other sequenced hav strains by about 20% at the nucleotide level and 7% at the amino acid level. two amino acid residues (residues 70 of vp3 and 102 of vp1), previously identified as constituting an immunodominant site and conserved in all sequenced hav ... | 1991 | 1649902 |
| [the modelling of hepatitis a in macaques]. | characteristics of experimental hepatitis a in macaca fascicularis and m. mulatta produced with hav, strain mp, isolated from m. mulatta in an outbreak of spontaneous hepatitis are presented. the hav-mp strain induced the disease in all the animals used in the experiment. the infection was manifest, with all virological, serological, biochemical and morphological features typical of hepatitis a. in m. mulatta, the process was protracted, with virus persistence in feces for at least 4 months. mod ... | 1991 | 1650062 |
| angiotensin converting enzyme in the monkey (macaca fascicularis) brain visualized by in vitro autoradiography. | angiotensin converting enzyme is localized in the monkey (macaca fascicularis) brain by in vitro autoradiography using the radiolabelled inhibitor, [125i]351a. this radioligand binds with high affinity and specificity to monkey cortical sections. specific inhibitors of converting enzyme, lisinopril and perindoprilat complete for the radioligand binding to monkey cortex sections with inhibitory constants of 10 nm. high concentrations of angiotensin converting enzyme occur in most components of th ... | 1991 | 1654536 |
| update: nonhuman primate importation. | beginning in november 1989, a number of cynomolgus monkeys (macaca fascicularis) imported into the united states were found to have been infected with a previously unrecognized ebola-like filovirus. this report summarizes findings of surveillance and serologic testing of nonhuman primates imported under special permits from june 1990 through september 1991. | 1991 | 1656185 |
| immunolocalization of n-acetylgalactosaminylphosphotransferase in the adult retina and subretinal space. | the cell surface n-acetylgalactosaminylphosphotransferase (galnacptase) modulates n-cadherin-mediated adhesion among embryonic chick retinal cells (balsamo et al., 1990). we are investigating the potential role of this transferase in modulating adhesive interactions in the adult retina. using a previously characterized monoclonal anti-galnacptase, we have used immunohistochemical and immunoblot techniques to localize and characterize the transferase in the retinas of the post metamorphic frog (x ... | 1991 | 1657627 |
| evidence for polymorphonuclear leukocyte collagenase and 92-kilodalton gelatinase in gingival crevicular fluid. | analysis of inflammatory exudate collected from sites of experimental periodontitis in cynomolgus monkeys has revealed the presence of collagenase and a 92-kda gelatinase that comigrated after electrophoresis with the 92-kda gelatinase released from polymorphonuclear leukocytes. since neutralizing antibodies to fibroblast collagenase had no effect on the collagenase activity and bacterial collagenases could not be detected, polymorphonuclear leukocytes appear to be the major source of collagenol ... | 1991 | 1657787 |
| social behavior and gender in biomedical investigations using monkeys: studies in atherogenesis. | we review the use of socially housed cynomolgus monkeys (macaca fascicularis) in biomedical research with emphasis on studies of atherosclerosis, particularly in the two specific domains of atherosclerosis investigation for which nonhuman primates are especially well-suited as animal models: gender differences and psychosocial influences. we found that the presence of normal ovarian function prevented exacerbation of diet-induced coronary artery atherosclerosis in female monkeys. however, any ma ... | 1991 | 1658480 |
| herpesvirus simiae (b virus) antibody response and virus shedding in experimental primary infection of cynomolgus monkeys. | four cynomolgus monkeys (macaca fascicularis) were inoculated in the lips and tongues with b virus. virus shedding and antibody responses were monitored for up to 50 days postinfection. virus was isolated from the oral cavities of all monkeys at 6 days postinfection despite the absence of observable lesions. virus was not isolated from genital swabs or serum. antibodies to both b virus and herpes simplex virus were detected by neutralization between days 8 and 12. virus-specific igm and igg anti ... | 1991 | 1658484 |
| monkey model of severe volume-controlled hemorrhagic shock with resuscitation to outcome. | seventeen cynomolgus monkeys under n2o analgesia and sedation were subjected to severe volume-controlled hemorrhagic shock (shed blood volume of 21 or 27 ml/kg). in 12 monkeys, resuscitation was started after increasing periods of hemorrhagic shock from 30 min to 5 h. in five additional monkeys, volume-controlled hemorrhage was modified at hemorrhagic shock 30 min to control map at 30 mmhg: resuscitation was started at hemorrhagic shock of 2 h. a clinically relevant resuscitation protocol consis ... | 1991 | 1658892 |
| evaluation of sq 28,603, an inhibitor of neutral endopeptidase, in conscious monkeys. | the potent neutral endopeptidase inhibitor sq 28,603 (n-(2-(mercaptomethyl)-1-oxo-3-phenylpropyl)-beta-alanine) significantly increased excretion of sodium from 4.9 +/- 2.3 to 14.3 +/- 2.1 muequiv./min and cyclic 3',5'-guanosine monophosphate from 118 +/- 13 to 179 +/- 18 pmol/min after intravenous administration of 300 mumol/kg (approximately 80 mg/kg) in conscious female cynomolgus monkeys. sq 28,603 did not change blood pressure or plasma atrial natriuretic peptide concentrations in the norma ... | 1991 | 1663819 |
| systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease. | systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ... | 1991 | 1665573 |
| the role of branhamella catarrhalis in the "bloody-nose syndrome" of cynomolgus macaques. | during a 15-month period, 25 cynomolgus macaques (macaca fascicularis) at the johns hopkins university were observed to have nasal discharge. fifteen (60%) of these animals had positive nasal cultures for branhamella catarrhalis. clinical signs associated with infection by this bacterium were sneezing, epistaxis, and mucohemorrhagic nasal discharge. treatment with antibiotics resulted in prompt resolution of clinical signs. post-therapeutic nasal cultures were negative for b. catarrhalis. two gr ... | 1991 | 1666136 |
| partial lobectomy via a ligature fracture technique: a method for multiple hepatic biopsies in nonhuman primates. | a ligature fracture technique was used to obtain multiple large (2 to 4 g) liver biopsy samples in both african green and cynomolgus monkeys. the technique was performed 195 times in 84 animals using three different surgical approaches, with no associated illness or mortality. in a subset of 18 animals, a slight decline in hematocrit percentage was noted during 14 days postsurgery (44.6 to 39.4%), but total plasma protein remained unchanged (5.98 to 5.95 g/dl). serum alanine aminotransferase con ... | 1991 | 1666152 |
| fluorometric determination of an inverse agonist to benzodiazepine receptors s-135 in monkey plasma by high performance liquid chromatography. | a high performance liquid chromatographic method for the determination of an agent of benzodiazepine receptor inverse agonist (s-135) was developed. s-135 in monkey plasma, after its administration, was assayed using the method. s-135 is ionized in alkaline n,n-dimethylformamide (dmf), and fluoresces strongly at 490 nm on excitation at 290 nm. extracted s-135 from a plasma sample was separated with an asahipak gs-310h polyvinyl alcohol gel column and detected fluorometrically, with the eluate be ... | 1991 | 1666862 |
| reference blood values of iron metabolism in cynomolgus macaques. | iron deficiency anemia is a human health problem of global significance, particularly as it affects pregnant women and infants. while the study of nonhuman primates has resulted in valuable knowledge about iron metabolism, hematologic and biochemical reference ranges for the parameters of iron metabolism are difficult to document in healthy monkeys. at our institution, we maintain a large breeding colony of healthy cynomolgus monkeys (macaca fascicularis). data compiled after sampling nonpregnan ... | 1991 | 1667207 |
| a pharmacokinetic study of e-4441, a new quinolone, in the rat, mouse and cynomolgus monkey. | the purpose of this present work has been to study the pharmacokinetical profile of e-4441 in the rat, mouse and cynomolgus monkey. analytical determination of the levels in plasma, urine and organs was affected by two different techniques: a microbiological agar diffusion assay with bacillus subtilis, and hplc with preliminary extraction in chloroform (ph 8.5). the kinetic behaviour of the unchanged substance was similar in the three animal species studied. absorption and excretion took place r ... | 1991 | 1668199 |
| salivary iga responses to porphyromonas gingivalis in the cynomolgus monkey. 1. total iga and iga antibody levels to p. gingivalis. | porphyromonas gingivalis has been associated with the subgingival plaque of advancing disease lesions in various types of periodontitis. additionally, this species of oral microorganism has been found to increase dramatically in ligature-induced periodontitis in nonhuman primates (macaca fascicularis) and has recently been shown to induce progressing disease when implanted into the subgingival plaque in this animal model. although systemic antibody responses have been demonstrated to p. gingival ... | 1991 | 1668248 |
| functional consequences of anti-icam-1 (cd54) in cynomolgus monkeys with renal allografts. | | 1991 | 1671301 |
| monoclonal antibodies against intercellular adhesion molecule 1 prolong cardiac allograft survival in cynomolgus monkeys. | | 1991 | 1671305 |
| ncq 298, a new selective iodinated salicylamide ligand for the labelling of dopamine d2 receptors. | ncq 298 ((s)-3-iodo-n-[(l-ethyl-2-pyrrolidinyl)methyl]-5,6- dimethoxysalicylamide) has an iodine substituent. we have labelled ncq 298 with 123i and 125i, and used the radioligands as tracers in receptor studies in vitro, in vivo in autoradiography and in spect studies on cynomolgus monkeys. [125i]ncq 298 bound in vitro to a single binding site with a kd = 19 pm. ncq 298 has thus a 10-fold higher affinity for the dopamine d2 receptors than the corresponding des-5-methoxy compound fla 961 (ibzm), ... | 1991 | 1672460 |
| 11c-sch 39166, a selective ligand for visualization of dopamine-d1 receptor binding in the monkey brain using pet. | the new selective d1-dopamine receptor antagonist sch 39166 was labelled with the positron emitting isotope 11c and used as ligand for visualization of dopamine-d1 receptor binding in cynomolgus monkeys by pet. after intravenous administration of the ligand a marked uptake of radioactivity was recorded in the d1-dopamine receptor-rich striatum and neocortex but not in the dopamine receptor-poor cerebellum. the uptake of radioactivity in striatum and neocortex was markedly displaced after the int ... | 1991 | 1674157 |
| effect of h2-receptor antagonists on the pharmacokinetics of 5-fluorouracil in the rat and monkey. | the effect of short term (7 days) and long term (28 days) pretreatment with the imidazole h2-receptor antagonist, cimetidine (cmt), on the pharmacokinetics of 5-fluorouracil (5-fura) has been studied in the rat and cynomolgus monkey. short-term pretreatment of rats with cmt significantly increased t1/2.z by 29 per cent and auc by 40 per cent: total body clearance was decreased by 30 per cent. long-term pretreatment exaggerated these effects. by contrast, short- and long-term pretreatment with th ... | 1991 | 1674889 |
| antibody response to the negative regulatory factor (nef) in experimentally infected macaques: correlation with viremia, disease progression, and seroconversion to structural viral proteins. | the antibody response to structural and regulatory viral proteins was studied in 14 rhesus (macaca mulatta) and 6 cynomolgus (macaca fascicularis) macaques experimentally infected with hiv-2 or sivmac. to investigate the humoral antibody response to the negative regulatory factor (nef), the recombinant protein was expressed to high levels with recombinant vaccinia virus (vv). nef-specific antibodies were detected in 14 of 20 infected macaques (70%). in sera of all infected monkeys antibodies dir ... | 1991 | 1675823 |
| plasma pharmacokinetics and metabolism of 13-cis- and all-trans-retinoic acid in the cynomolgus monkey and the identification of 13-cis- and all-trans-retinoyl-beta-glucuronides. a comparison to one human case study with isotretinoin. | in order to compare the disposition and metabolism of 13-cis-retinoic acid (13-cis-ra) and all-trans-retinoic acid (all-trans-ra) in the nonpregnant female cynomolgus monkey, the plasma concentrations of the parent compound, the oxidized metabolites 4-oxo-13-cis-retinoic acid and 4-oxo-all-trans-retinoic acid, and the conjugate metabolites 13-cis-retinoyl-beta-glucuronide (13-cis-rag) and all trans-retinoyl-beta-glucuronide (all-trans-rag), were determined on day 1 and day 10 after oral dosing o ... | 1991 | 1676630 |
| abnormal spontaneous activity of globus pallidus neurons in monkeys with mptp-induced parkinsonism. | the goal of the study was to determine abnormalities in the spontaneous activity of globus pallidus neurons at the output of the basal ganglia, in cynomolgus monkeys rendered parkinsonian by the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). in parkinsonian compared to intact monkeys, the mean spontaneous firing rate of the neurons of the internal segment of the globus pallidus (gpi) increased but that of the prevailing neuronal population in the external segment (gpe) inversely ... | 1991 | 1677607 |
| effects of dopamine agonists on the spontaneous activity of globus pallidus neurons in monkeys with mptp-induced parkinsonism. | the mixed (d1 and d2) dopamine agonist apomorphine was injected (10-200 micrograms/kg, s.c.) to cynomolgus monkeys before and after they were rendered parkinsonian by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). motor behavior was examined together with corresponding neuronal activity in the external (gpe) and internal (gpi) segments of the globus pallidus, including a small population of neurons localized within the gpe and displaying a characteristic discharge at low frequency with bur ... | 1991 | 1677608 |
| prevention of hiv-2 and sivsm infection by passive immunization in cynomolgus monkeys. | infection of macaques with simian immunodeficiency virus (siv) and human immunodeficiency virus type 2 (hiv-2) are useful models for studies of immunotherapy and vaccination against hiv as well as for testing of antiviral drugs. vaccine research showing protective immunity in immunized monkeys has indicated that it will be possible to develop a vaccine for prevention of human hiv infection, although many hurdles remain. the design of an hiv vaccine would be helped if the basis of the protective ... | 1991 | 1677743 |