| immunohistochemical characterization of polyclonal antibodies against enteromyxum leei and enteromyxum scophthalmi (myxozoa: myxosporea), intestinal parasites of fish. | the enteric myxozoan parasites enteromyxum leei (diamant, lom et dyková) and enteromyxum scophthalmi palenzuela, redondo et álvarez-pellitero are responsible for high weight loss in infected fish, which leads to subchronic disease and low mortality rates in gilthead sea bream (gsb), sparus aurata l., and to high mortality rates in turbot, psetta maxima (l.). the detection of initial parasite stages in histological sections is particularly difficult, but can be simplified by means of specific ant ... | 2014 | 23957839 |
| naturally occurring stable isotopes reflect changes in protein turnover and growth in gilthead sea bream (sparus aurata) juveniles under different dietary protein levels. | ideal nutritional conditions are crucial to sustainable aquaculture due to economic and environmental issues. here we apply stable isotope analysis as an indicator of fish growth and feeding balance, to define the optimum diet for efficient growing conditions. juveniles of gilthead sea bream were fed with six isoenergetic diets differing in protein to lipid proportion (from 41/26 to 57/20). as protein intake increased, δ¹⁵n and δδ¹⁵n of muscle and δδ¹⁵n and δδ¹³c of its protein fraction decrease ... | 2013 | 23947425 |
| carbonic anhydrase activity from the gilthead sea bream (sparus aurata) liver: the toxicological effects of heavy metals. | many studies have shown that metal ions may lead to oxidative stress in biological systems. accordingly, dna damage, protein modification, enzyme inhibition and activation, lipid peroxidation and many other effects may occur in living organisms. many different formations of metal ions may enter human cells along with water, air, and various foods, and humans are negatively affected by these conditions, either directly or indirectly. these effects may cause irreversible damage to human metabolism ... | 2013 | 23811108 |
| comparative analysis of a teleost skeleton transcriptome provides insight into its regulation. | an articulated endoskeleton that is calcified is a unifying innovation of the vertebrates, however the molecular basis of the structural divergence between terrestrial and aquatic vertebrates, such as teleost fish, has not been determined. in the present study long-read next generation sequencing (ngs, roche 454 platform) was used to characterize acellular perichondral bone (vertebrae) and chondroid bone (gill arch) in the gilthead sea bream (sparus auratus). a total of 15.97 and 14.53mb were pr ... | 2013 | 23770218 |
| status and future perspectives of vaccines for industrialised fin-fish farming. | fin fish farming is developing from extensive to intensive high industrial scale production. production of fish in high-density growth conditions requires effective vaccines in order to control persistent and emerging diseases. vaccines can also have significant positive impact on the reduced usage of antibiotics. this was demonstrated when vaccines were introduced in norway for atlantic salmon (salmo salar) in the late eighties and early nineties, resulting in a rapid decline of antibiotics con ... | 2013 | 23769873 |
| pthrp-induced modifications of the sea bream (sparus auratus) vertebral bone proteome. | endocrine factors play an essential role in the formation and turnover of the skeleton in vertebrates. in the present study sea bream vertebral bone transcripts for pth1r and pth3r were identified and the action of intermittent administration of parathyroid hormone related protein (pthrp) on the proteome of vertebral bone was analysed. treatment of immature sea bream (sparus auratus, n=6) for 5days with homologous recombinant pthrp(1-125; 150ng/g body weight) modified bone metabolism and caused ... | 2013 | 23747812 |
| effect of partially protected butyrate used as feed additive on growth and intestinal metabolism in sea bream (sparus aurata). | butyrate is a short-chain fatty acid extensively used in animal nutrition since it promotes increases in body weight and other multiple beneficial effects on the intestinal tract. although such effects have been demonstrated in several species, very few studies have assessed them in fish. on the other hand, little is known about the metabolic processes underlying these effects. in the present work, growth parameters and changes in more than 80 intestinal metabolites (nucleotides, amino acids and ... | 2013 | 23737146 |
| proteomic approach to skin regeneration in a marine teleost: modulation by oestradiol-17β. | skin and scale formation and regeneration in teleosts have mainly been described from a morphological perspective, and few studies of the underlying molecular events exist. the present study evaluates (1) the change in the skin proteome during its regeneration in a marine teleost fish (gilthead sea bream, sparus aurata) and (2) the impact of oestradiol-17β (ε2) on regeneration and the involvement of oestrogen receptor (er) isoforms. thirty-five candidate proteins were differentially expressed (p ... | 2013 | 23728848 |
| light and feeding entrainment of the molecular circadian clock in a marine teleost (sparus aurata). | daily light and feeding cycles act as powerful synchronizers of circadian rhythmicity. ultimately, these external cues entrain the expression of clock genes, which generate daily rhythmic behavioral and physiological responses in vertebrates. in the present study, we investigated clock genes in a marine teleost (gilthead sea bream). partial cdna sequences of key elements from both positive (bmal1, clock) and negative (per2, cry1) regulatory loops were cloned before studying how feeding time affe ... | 2013 | 23688119 |
| effects of dietary energy density and digestible protein:energy ratio on de novo lipid synthesis from dietary protein in gilthead sea bream (sparus aurata) quantified with stable isotopes. | the effects of varying dietary digestible protein (dp) and digestible energy (de) content on performance, nutrient retention efficiency and the de novo lipogenesis of dp origin were examined in triplicate groups of gilthead sea bream (sparus aurata), fed nine extruded experimental diets. in order to trace the metabolic fate of dietary protein, 1·8% fishmeal was replaced with isotope-labelled whole protein (.98% 13c). the experiment was divided into a growth period lasting 89 d, growing fish from ... | 2013 | 23631822 |
| characterization and endocrine regulation of proliferation and differentiation of primary cultured preadipocytes from gilthead sea bream (sparus aurata). | a preadipocyte primary cell culture was established to gain knowledge about adipose tissue development in gilthead sea bream (sparus aurata), one of the most extensively produced marine aquaculture species in the mediterranean. the preadipocytes obtained from the stromal-vascular cell fraction of adipose tissue proliferated in culture, reaching confluence around day 8. at that time, the addition of an adipogenic medium promoted differentiation of the cells into mature adipocytes, which showed an ... | 2013 | 23535263 |
| element levels in cultured and wild sea bass (dicentrarchus labrax) and gilthead sea bream (sparus aurata) from the adriatic sea and potential risk assessment. | in this study, the role of aquaculture activity as a source of selected metals was analyzed. significant differences in element content between cultured (dicentrarchus labrax, sparus aurata) and wild fishes as well as between fish muscle and their feed were detected. higher concentrations of trace elements (i.e., as, cu, hg, se) in wild fish tissues in comparison with cultured ones indicate additional sources of metals beside fish feed as natural and/or anthropogenic sources. generally, mean cd, ... | 2014 | 23504489 |
| insulin-like growth factors effects on the expression of myogenic regulatory factors in gilthead sea bream muscle cells. | gilthead sea bream (sparus aurata) is a widely cultured fish; however, muscle development regulation is poorly known. myogenesis can be activated by the myogenic regulatory factors (mrfs: myod, myf5, myogenin and mrf4) and by endocrine signals from the growth hormone (gh)/insulin-like growth factors (igfs) axis. we cultured gilthead sea bream myocytes to better understand the role of igfs in muscle growth and differentiation through the regulation of mrfs expression. first, we studied the expres ... | 2013 | 23500676 |
| deep sequencing for de novo construction of a marine fish (sparus aurata) transcriptome database with a large coverage of protein-coding transcripts. | the gilthead sea bream (sparus aurata) is the main fish species cultured in the mediterranean area and constitutes an interesting model of research. nevertheless, transcriptomic and genomic data are still scarce for this highly valuable species. a transcriptome database was constructed by de novo assembly of gilthead sea bream sequences derived from public repositories of mrna and collections of expressed sequence tags together with new high-quality reads from five cdna 454 normalized libraries ... | 2013 | 23497320 |
| gill morphology in two mediterranean sea fishes of similar feeding preferences: sea bream (sparus aurata l) and sea bass (dicentrarchus labrax). | the aim of the present study was to describe the gill morphology of the gilthead sea bream and sea bass fishes that showed the same feeding habits. our study focused on the gross morphological, scanning and light microscopical features of the gills, where a many differences in the gill arches and gill rakers surface in both species were explained. our results marked that; there was an interbranchial median elevation appeared in sea bream. the gill arches length and the gaps between decreased med ... | 2013 | 23475751 |
| dietary oils mediate cortisol kinetics and the hepatic mrna expression profile of stress-responsive genes in gilthead sea bream (sparus aurata) exposed to crowding stress. implications on energy homeostasis and stress susceptibility. | juveniles of gilthead sea bream were fed with plant protein-based diets with fish oil (fo diet) or vegetable oils (66vo diet) as dietary lipid sources. no differences in growth performance were found between both groups, and fish with an average body mass of 65-70 g were crowded (90-100 kg/m(3)) to assess the stress response within the 72 h after the onset of stressor. the rise in plasma cortisol and glucose levels was higher in stressed fish of group 66vo (66vo-s) than in fo group (fo-s), but t ... | 2013 | 23466468 |
| evaluation of sea bream (sparus aurata) shelf life using an optoelectronic nose. | a new optoelectronic nose for the shelf-life assessment of fresh sea bream in cold storage has been developed. the chromogenic array used eight sensing materials (based on aluminium oxide and silica gel) containing ph indicators, lewis acids and an oxidation-reduction indicator. the colour changes of the sensor array were characteristic of sea bream spoilage. colour modulations were measured on day 0 and for the samples held in cold storage for 2, 4, 7, 9 and 11 days. determination of moisture c ... | 2013 | 23411257 |
| multidisciplinary analytical investigation of phospholipids and triglycerides in offshore farmed gilthead sea bream (sparus aurata) fed commercial diets. | in this work, a quantitative characterisation of lipid (both triglycerides and phospholipids) rearrangements in the muscle of offshore-raised gilthead sea bream was carried out as a function of fish growth between april and september. relative percentages of lipid classes and fatty acids/acyls composition of the commercial feeds and fish dorsal muscles were assessed by means of an interdisciplinary analytical approach. a combination of preparative chemistry and experimental results from nmr spec ... | 2013 | 23411224 |
| taurine and fish development: insights for the aquaculture industry. | expansion of the aquaculture industry is limited by incomplete knowledge on fish larval nutritional requirements. nevertheless, it is believed that dietary taurine deficiencies may be particularly critical for fish larvae. the reasons include the high taurine levels found during egg and yolk-sac stages of fish, suggesting that taurine may be of pivotal importance for larval development. moreover, unlike aquaculture feeds, natural preys of fish larvae contain high taurine levels, and dietary taur ... | 2013 | 23392894 |
| florfenicol depletion in edible tissue of rainbow trout, oncorhynchus mykiss (walbaum), and sea bream, sparus aurata l. | an increase in fish production has consequently brought an increase in infectious diseases in fish farms. the use of chemotherapic drugs is the most effective instrument against common bacterial agents. the number of registered drugs for use in aquaculture is limited and often veterinary practitioners resort to the off-label use of chemotherapic agents authorized for different food-producing animal species. florfenicol is well known for its outstanding effect against various pathogenic bacteria ... | 2013 | 23384074 |
| effects on the skeleton development in reared gilthead sea bream (sparus aurata). | | 2013 | 25141718 |
| ontogeny of kiss2 and kiss1r gene expression in gilthead sea bream (sparus aurata) larvae. | | 2013 | 25141705 |
| evaluation of daily rhythms in feeding activity and digestive functions in gilthead sea bream (sparus (sparus aurata) larvae. | | 2013 | 25141687 |
| expression of skeletal myosin light chain 2 in gilthead sea bream (sparus aurata, l): regulation and correlation to growth markers. | | 2013 | 25141652 |
| seasonal variations of cellular stress response of the gilthead sea bream (sparus aurata). | the present study aimed to investigate the seasonal cellular stress response in vital organs, like the heart, the liver, the whole blood and the skeletal (red and white) muscles of the mediterranean fish sparus aurata during a 1-year acclimatization period in the field, in two examined depths (0-2 m and 10-12 m). processes studied included heat shock protein expression and protein kinase activation. molecular responses were addressed through the expression of hsp70 and hsp90, the phosphorylation ... | 2013 | 23269615 |
| levels of (210)po in some commercial fish species consumed in the aegean sea coast of turkey and the related dose assessment to the coastal population. | concentrations of (210)po were determined in the edible muscle tissue of five species of marine fish: sardine (sardine plichardus) and anchovy (engraulis encrasicolus), red mullet (mullus barbatus), horse mackerel (trachurus trachurus) and gilt-head sea bream (sparus aurata) collected from five stations in the aegean sea region of turkey during the fishery season 2010. the (210)po concentrations in the fish samples were found to vary from not detected levels to 389 ± 45 bq kg(-1) dry wt. these v ... | 2013 | 23268115 |
| glycogenesis and de novo lipid synthesis from dietary starch in juvenile gilthead sea bream (sparus aurata) quantified with stable isotopes. | the effects of replacing a digestible energy source from fat (fish oil) with carbohydrate (wheat starch) on performance, glycogenesis and de novo lipogenesis was examined in triplicate groups of juvenile gilthead sea bream (sparus aurata), fed four extruded experimental diets. in order to trace the metabolic fate of dietary starch, 0.7% wheat starch was replaced with isotope-labelled starch (>98% 13c). fish were fed the experimental diets for three consecutive 10 d periods, and isotope ratio ms ... | 2013 | 23186693 |
| neuropeptides isotocin and arginine vasotocin in urophysis of three fish species. | in this study, for the first time, both neuropeptides isotocin (it) and arginine vasotocin (avt) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. so far, avt, but not it, was quantified by radioimmunoassay (ria) in urophysis of several fish species. we have used high-performance liquid chromatographic assay with fluorescence detection (hplc-fl) preceded by solid-phase extraction (spe) and liquid chromatography-electrospray ... | 2013 | 23142930 |
| microsatellite length variation in candidate genes correlates with habitat in the gilthead sea bream sparus aurata. | the genetic basis and evolutionary implications of local adaptation in high gene flow marine organisms are still poorly understood. in several mediterranean fish species, alternative migration patterns exist between individuals entering coastal lagoons that offer favourable conditions for growth and those staying in the sea where environmental conditions are less subject to rapid and stressful change. whether these coexisting strategies are phenotypically plastic or include a role for local adap ... | 2012 | 23061421 |
| effects of diet supplementation with white tea and methionine on lipid metabolism of gilthead sea bream juveniles (sparus aurata). | a growth trial was performed with gilthead sea bream juveniles (sparus aurata) to evaluate the effect of diet supplementation with white tea and methionine on fish performance and lipid metabolism. for that purpose, four diets were formulated: a fish meal-based diet (control) and diets identical to the control diet but supplemented with 2.9 % white tea (tea), 0.3 % methionine (met) or 2.9 % white tea plus 0.3 % methionine (tea + met). growth performance and feed efficiency parameters, whole-body ... | 2013 | 23053611 |
| cadmium exposure affects the expression of genes involved in skeletogenesis and stress response in gilthead sea bream larvae. | gilthead sea bream larvae (sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of cd(2+) (0.1, 5 and 10 mg/l) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (metallothionein-mt, glutathione peroxidase 1-gpx1), stress response (heat shock protein 70-hsp70, tumour necrosis factor α-tnfα) and ossification (osteocalcin-oc) processes. for this ... | 2013 | 23053610 |
| expression of developmental-stage-specific genes in the gilthead sea bream sparus aurata l. | the mechanism of early fish development as well as the control of egg quality is of great importance for the ability of the oocyte to develop after fertilization. embryonic development is initially regulated by maternally provided mrnas and later by the zygotic genome. maternal mrnas have an important role in initiating processes crucial to patterning the developing fish embryo. furthermore, it has been shown that maternal rna plays an important role in egg quality. the identification and charac ... | 2013 | 23053055 |
| adaptation to different salinities exposes functional specialization in the intestine of the sea bream (sparus aurata l.). | the processing of intestinal fluid, in addition to a high drinking rate, is essential for osmoregulation in marine fish. this study analyzed the long-term response of the sea bream (sparus aurata l.) to relevant changes of external salinity (12, 35 and 55 p.p.t.), focusing on the anterior intestine and in the less-often studied rectum. intestinal water absorption, epithelial hco(3)(-) secretion and gene expression of the main molecular mechanisms (slc26a6, slc26a3, slc4a4, atp6v1b, cftr, nkcc1 a ... | 2013 | 23038737 |
| divergent responsiveness of the dentary and vertebral bone to a selective estrogen-receptor modulator (serm) in the teleost sparus auratus. | in teleosts the regulation of skeletal homeostasis and turnover by estrogen is poorly understood. for this reason raloxifene, a selective estrogen-receptor modulator (serm), was administered to sea bream (sparus auratus) and its effect on plasma calcium balance and transcript expression in dentary (dermal bone) and vertebra (perichondral bone) was studied. the concentration of total calcium or phosphorus in plasma was unchanged by raloxifene treatment for 6days. the activity of alkaline phosphat ... | 2012 | 23036732 |
| the goitrogenic efficiency of thioamides in a marine teleost, sea bream (sparus auratus). | studies on the role of thyroid hormones (ths) in teleost fish physiology have deployed the synthetic goitrogens, methimazol (mmi), propilthiouracil (ptu) and thiourea (tu) that are used to treat human hyperthyroidism. however, the action of the goitrogens, mmi, ptu and tu at different levels of the hypothalamic-pituitary-thyroid (hpt) axis in teleosts is largely unknown. the central importance of the hypothalamus and pituitary in a number of endocrine regulated systems and the cross-talk that oc ... | 2012 | 23032075 |
| effects of chemical and handling exposure on fatty acids, oxidative stress and morphological welfare indicators in gilt-head sea bream (sparus aurata). | this work investigates the changes in the morphological traits, body composition, body fat and oxidative stress of sparus aurata under two stress conditions: a low weekly exposure to the pesticide diuron and chronic handling stress. the fish exposed to handling stress showed lower values in the morphological traits and perivisceral fat, whereas chemical stress induced a decrease in the percentage of muscle and carcase and an increase in the percentage of spleen and hepatosomatic index. the two s ... | 2013 | 23010939 |
| dietary vegetable oils do not alter the intestine transcriptome of gilthead sea bream (sparus aurata), but modulate the transcriptomic response to infection with enteromyxum leei. | studies conducted with gilthead sea bream (sparus aurata l.) have determined the maximum dietary replacement of fish meal and oil without compromising growth or product quality. the present study aimed to analyze the effect of the nutritional background on fish health and fish fed plant protein-based diets with fish oil (fo diet) or a blend of vegetable oils (66vo diet) were exposed for 102 days to the intestinal myxosporean parasite enteromyxum leei, and the intestine transcriptome was analyzed ... | 2012 | 22967181 |
| beneficial effects of sustained activity on the use of dietary protein and carbohydrate traced with stable isotopes 15n and 13c in gilthead sea bream (sparus aurata). | to determine the effects of sustained swimming on the use and fate of dietary nutrients in gilthead sea bream, a group of fish were forced to undertake moderate and sustained swimming (1.5 bl s(-1)) for 3 weeks and compared with a control group undertaking voluntary activity. the exercise group showed a significant increase in specific growth rate (c: 1.13 ± 0.05; e: 1.32 ± 0.06 % day(-1), p < 0.05) with no significant change in food intake (c: 3.56 ± 0.20; e: 3.84 ± 0.03 % of body weight). the ... | 2013 | 22918602 |
| effect of nutrition and enteromyxum leei infection on gilthead sea bream sparus aurata intestinal carbohydrate distribution. | the effect of a practical plant protein-based diet containing vegetable oils (vo) as the major lipid source on the mucosal carbohydrate pattern of the intestine was studied in gilthead sea bream sparus aurata challenged with the myxosporean parasite enteromyxum leei. fish fed for 9 mo either a fish oil (fo) diet or a blend of vo at 66% of replacement (66vo diet) were exposed to parasite-contaminated water effluent. samples of the anterior, middle and posterior intestine (ai, mi and pi, respectiv ... | 2012 | 22885511 |
| sodium alginate as feed additive in cultured sea bream (sparus aurata): does it modify the quality of the flesh? | the objective of this study was to evaluate the effect of sodium alginate obtained from brown seaweed as a prebiotic supplement to the feed of reared sea bream (sparus aurata). addition of the alginate to a control diet was investigated at both concentrations 2% and 5%. proximate composition in the flesh were not modified significantly by sodium alginate inclusion in the diet of the sea bream; however the fat and ash content in the specimens supplemented with 5% alginate were found to be signifi ... | 2012 | 22868148 |
| effect of ration size on fillet fatty acid composition, phospholipid allostasis and mrna expression patterns of lipid regulatory genes in gilthead sea bream (sparus aurata). | the effect of ration size on muscle fatty acid (fa) composition and mrna expression levels of key regulatory enzymes of lipid and lipoprotein metabolism have been addressed in juveniles of gilthead sea bream fed a practical diet over the course of an 11-week trial. the experimental setup included three feeding levels: (i) full ration until visual satiety, (ii) 70 % of satiation and (iii) 70 % of satiation with the last 2 weeks at the maintenance ration. feed restriction reduced lipid content of ... | 2013 | 22856503 |
| prolactin regulates luminal bicarbonate secretion in the intestine of the sea bream (sparus aurata l.). | the pituitary hormone prolactin is a pleiotropic endocrine factor that plays a major role in the regulation of ion balance in fish, with demonstrated actions mainly in the gills and kidney. the role of prolactin in intestinal ion transport remains little studied. in marine fish, which have high drinking rates, epithelial bicarbonate secretion in the intestine produces luminal carbonate aggregates believed to play a key role in water and ion homeostasis. the present study was designed to establis ... | 2012 | 22855618 |
| effects of chronic cortisol administration on global expression of gr and the liver transcriptome in sparus aurata. | the present work was designed to assess the effects of artificially increased high plasma cortisol levels induced by slow-release cortisol implants on the mrna abundance of the glucocorticoid receptor (gr) in different organs of sparus aurata (gilthead sea bream), as well as to evaluate global transcriptional changes in the liver, using the aquagenomics s. aurata oligo-nucleotide microarray technology. for that purpose, groups of fish were intraperitoneally injected with implants containing two ... | 2013 | 22777624 |
| water absorption and bicarbonate secretion in the intestine of the sea bream are regulated by transmembrane and soluble adenylyl cyclase stimulation. | in the marine fish intestine luminal, hco₃⁻ can remove divalent ions (calcium and magnesium) by precipitation in the form of carbonate aggregates. the process of epithelial hco₃⁻ secretion is under endocrine control, therefore, in this study we aimed to characterize the involvement of transmembrane (tmacs) and soluble (sacs) adenylyl cyclases on the regulation of bicarbonate secretion (bcs) and water absorption in the intestine of the sea bream (sparus aurata). we observed that all sections of s ... | 2012 | 22752677 |
| modulation of the igm gene expression and igm immunoreactive cell distribution by the nutritional background in gilthead sea bream (sparus aurata) challenged with enteromyxum leei (myxozoa). | the aim of the present work was to determine if a plant protein-based diet containing vegetable oils (vo) as the major lipid source could alter the distribution of igm immunoreactive cells (ircs) and the igm expression pattern in the intestine and haematopoietic tissues of gilthead sea bream (gsb) (sparus aurata) challenged with the myxosporean enteromyxum leei. in a first trial (t1), gsb fed for 9 months either a fish oil (fo) diet or a blend of vo at 66% of replacement (66vo diet) was challeng ... | 2012 | 22659442 |
| metabolic effects of insulin and igfs on gilthead sea bream (sparus aurata) muscle cells. | primary cultures of gilthead sea bream myocytes were performed in order to examine the relative metabolic function of insulin compared with igf-i and igf-ii (insulin-like growth factors, igfs) at different stages in the cell culture. in these cells, the in vitro effects of insulin and igfs on 2-deoxyglucose (2-dg) and l-alanine uptake were studied in both myocytes (day 4) and small myotubes (day 9). 2-dg uptake in gilthead sea bream muscle cells was increased in the presence of insulin and igfs ... | 2012 | 22654873 |
| involvement of p38 mapk in the induction of hsp70 during acute thermal stress in red blood cells of the gilthead sea bream, sparus aurata. | the present study aimed to examine the expression and activation of mapks (p38 mapk, erk1/2, and jnks) in red blood cells (rbcs) of the gilthead sea bream, sparus aurata, during thermal stress and investigate their involvement in the expression of heat shock proteins. the data showed that only p38 mapk is detected in rbcs of sparus aurata and it is phosphorylated and activated during exposure to increased temperature. induction of hsp70 in thermally stressed rbcs was abolished in the presence of ... | 2012 | 25363579 |
| cloning and expression pattern of facilitative glucose transporter 1 (glut1) in gilthead sea bream sparus aurata in response to salinity acclimation. | facilitative glucose transporters (glut) are transmembrane transporter proteins involved in glucose transport across the plasma membrane. in fish, several glut mrnas have been cloned, but to date there is no information about these transporters in the marine euryhaline teleost sparus aurata. in the present study we obtained the complete coding sequence from s. aurata glut1 (saglut1), composed by 4483 bases, presenting a 79 to 95% identity with respect to other fish glut1 mrnas. the analysis of t ... | 2012 | 22580053 |
| structure, tissue distribution and estrogen regulation of splice variants of the sea bream estrogen receptor α gene. | estrogen actions are mainly mediated by specific nuclear estrogen receptors (ers), for which different genes and a diversity of transcript variants have been identified, mainly in mammals. in this study, we investigated the presence of er splice variants in the teleost fish gilthead sea bream (sparus auratus), by comparison with the genomic organization of the related species takifugu rubripes. two exon2-deleted erα transcript variants were isolated from liver cdna of estradiol-treated fish. the ... | 2012 | 22579469 |
| fast skeletal muscle transcriptome of the gilthead sea bream (sparus aurata) determined by next generation sequencing. | the gilthead sea bream (sparus aurata l.) occurs around the mediterranean and along eastern atlantic coasts from great britain to senegal. it is tolerant of a wide range of temperatures and salinities and is often found in brackish coastal lagoons and estuarine areas, particularly early in its life cycle. gilthead sea bream are extensively cultivated in the mediterranean with an annual production of 125,000 metric tonnes. here we present a de novo assembly of the fast skeletal muscle transcripto ... | 2012 | 22577894 |
| elemental composition of four farmed fish produced in portugal. | farmed gilthead sea bream (sparus aurata), european sea bass (dicentrarchus labrax), rainbow trout (oncorhynchus mykiss) and turbot (psetta maxima) produced in portugal were analysed in order to characterize their elemental composition. atomic absorption (flame and cold vapour) and molecular absorption spectrometry techniques were used to determine all the studied elements. similar patterns of macro, trace and ultra trace elements were observed for all fish species. the main elements were potass ... | 2012 | 22574998 |
| discriminating farmed gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax from wild stocks through scales and otoliths. | the study of mass and standard length (l(s) ) relationships showed that farmed individuals had higher values than wild fishes for both gilthead sea bream sparus aurata and european sea bass dicentrarchus labrax. such differences were more pronounced in larger individuals than smaller ones and were more noticeable in s. aurata than in d. labrax. additionally, differences in external characteristics of scales were detected between origins. a high proportion of farmed s. aurata had a regenerated nu ... | 2012 | 22551175 |
| quantitative trait loci for body growth and sex determination in the hermaphrodite teleost fish sparus aurata l. | gilthead sea bream (sparus aurata l.) is an important marine fish in mediterranean aquaculture. sex determination by age and/or body weight is a critical life-history trait, the genetic basis for which is largely unknown in this sequential hermaphrodite species. herein, we performed a partial genome scan to map quantitative trait loci (qtl) affecting body weight and sex using 74 informative microsatellite markers from 10 paternal half-sib families to construct nine linkage groups (lg). in total, ... | 2012 | 22497460 |
| multiresidue determination of veterinary drugs in aquaculture fish samples by ultra high performance liquid chromatography coupled to tandem mass spectrometry. | a simple, selective and fast multiresidue method was developed for the determination of 32 veterinary drug residues belonging to several families, in gilthead sea bream (sparus aurata) by ultra high performance liquid chromatography coupled to tandem mass spectrometry (uhplc-ms/ms). the extraction was based on modified quechers (quick, easy, cheap, effective, rugged and safe) procedure, using as extraction solution a mixture of acetonitrile and methanol (75:25, v/v), and it reduces sample handli ... | 2012 | 22465276 |
| structural changes in marinated fillets of gilthead sea bream (sparus aurata l.) during vacuum storage at refrigerated temperature. | fillets of sparus aurata were marinated in a mixture of salt (5.5%) and citric acid (48.9 g/kg) for 1 h, then vacuum packed and refrigerated for 1, 5, 16, and 21 d. structural and ultrastructural parameters were evaluated in 6 specimens per time period. at day 1, the preservative solution appeared among the muscle fibers, thus increasing the interfibrillar spaces; cytoplasmic organelles were swollen and the sarcolemma-endomysium appeared very altered and electron-dense. at 5 to 10 d, the marinad ... | 2011 | 22417345 |
| metabolomics as a powerful tool for molecular quality assessment of the fish sparus aurata. | the molecular profiles of perchloric acid solutions extracted from the flesh of sparus aurata fish specimens, produced according to different aquaculture systems, have been investigated. the (1)h-nmr spectra of aqueous extracts are indicative of differences in the metabolite content of fish reared under different conditions that are already distinguishable at their capture, and substantially maintain the same differences in their molecular profiles after sixteen days of storage under ice. the fi ... | 2011 | 22254093 |
| the effect of hypoxia on intermediary metabolism and oxidative status in gilthead sea bream (sparus aurata) fed on diets supplemented with methionine and white tea. | the present study evaluates the influence of previous nutritional status, fish fed on diets supplemented with tea and methionine, on acute hypoxia tolerance and subsequent recovery of sparus aurata juveniles. four isonitrogenous (45% of protein) and isolipidic (18% lipid) diets were formulated to contain 0.3% methionine, 2.9% white tea dry leaves or 2.9% of white tea dry leaves+0.3% methionine. an unsupplemented diet was used as control. hepatic key enzymes of intermediary metabolism and antioxi ... | 2012 | 22227440 |
| comparison of two techniques for the morphometry study on gilthead seabream (sparus aurata) spermatozoa and evaluation of changes induced by cryopreservation. | the development of powerful software has made possible spermatozoa morphology studies. however, some problems have emerged in relation to protocol standardization to compare results from different laboratories. this study was carried out to compare two techniques commonly used (staining vs phase contrast technique) for the morphometry study of gilthead sea bream spermatozoa using an integrated sperm analysis system (isas). spermatozoa morphometry values were significantly affected by the techniq ... | 2012 | 22225681 |
| the effect of dietary methionine and white tea on oxidative status of gilthead sea bream (sparus aurata). | free radicals are continuously generated during an organism's lifetime. in order to understand the involvement in the oxidative status of fish, methionine and white tea were assayed as antioxidant supplements in diets for gilthead sea bream (sparus aurata). for the purpose of this study, four isonitrogenous and isolipidic diets were formulated to contain 45 % of protein and 18 % lipid and 0·3 % methionine (met diet), 2·9 % white tea dry leaves (tea diet) and 2·9 % of white tea dry leaves + 0·3 % ... | 2012 | 22153060 |
| aquaporin-4 immunoreactivity in müller and amacrine cells of marine teleost fish retina. | aquaporins (aqps) are membrane proteins that facilitate water transport across biological membranes and are essential for the proper function of neural tissue. although aqps have been extensively studied in mammalian retina, their presence in lower vertebrate retina is less frequently characterized. aqp4 expressed in mammalian and chick müller cells plays a major part in maintaining retinal homeostasis. in this study, we examined the immunoreactivity of aqp4 in the adult retina of gilthead sea b ... | 2012 | 22138430 |
| exploring neutral and adaptive processes in expanding populations of gilthead sea bream, sparus aurata l., in the north-east atlantic. | recent studies in empirical population genetics have highlighted the importance of taking into account both neutral and adaptive genetic variation in characterizing microevolutionary dynamics. here, we explore the genetic population structure and the footprints of selection in four populations of the warm-temperate coastal fish, the gilthead sea bream (sparus aurata), whose recent northward expansion has been linked to climate change. samples were collected at four atlantic locations, including ... | 2012 | 22126850 |
| effects of olive pomace and olive pomace oil on growth performance, fatty acid composition and cardio protective properties of gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax). | gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) were fed with two experimental diets: olive pomace diet and olive pomace oil diet, in order to examine whether fish oil substitution in fish feeds by olive oil production wastes, such as olive pomace and olive pomace oil, has an influence on growth performance, fatty acid composition and cardio protective properties of fish. gilthead sea bream fed with both experimental diets exhibited satisfactory growth performance factors ... | 2011 | 25212344 |
| gut ph as a limiting factor for digestive proteolysis in cultured juveniles of the gilthead sea bream (sparus aurata). | after the development of the gastric function in juvenile fish, dietary proteins enter a two-phase digestive process comprising an acidic gastric phase followed by an alkaline intestinal phase. however, the main gastric protease, pepsin, is strictly dependent on the existence of a low-enough environmental ph. in 20-g gilthead sea bream, sparus aurata, the mean minimal gastric ph is close to 4.5, while the mean ph in the duodenal portion of the intestine was nearly fixed at 6.5. the mean maximal ... | 2012 | 22086356 |
| heterozygosity-fitness correlations in the gilthead sea bream sparus aurata using microsatellite loci from unknown and gene-rich genomic locations. | heterozygosity-fitness correlations (hfc) were assessed for a sample of a gilthead sea bream sparus aurata population. two hundred and seventy-one fish were genotyped at 22 known and novel microsatellite loci, from which correlations between the multilocus heterozygosity index (i(mlh) ) and various fitness traits (fork length, mass and specific growth rates) were calculated. significant global hfcs were found in this sample (0·02 ≤r(2) ≤ 0·08). in addition, all the significant correlations found ... | 2011 | 22026596 |
| the effects of extraction methods on the contents of fatty acids, especially epa and dha in marine lipids. | this study aims to evaluate the efficiency of trans-methylation methods in fish oil obtained using soxhlet extraction method and bligh and dyer method and also to observe the effects of extraction methods on the contents of polyunsaturated fatty acids [pufas, especially eicosapentaenoic acid (epa) and docosahexaenoic acid (dha)] in five marine species: sardine (sardinella aurita), anchovy (engraulis encrasicolus), sea bream (sparus aurata), brushtooth lizardfish (saurida undosquamis) and chub ma ... | 2012 | 22010951 |
| skin healing and scale regeneration in fed and unfed sea bream, sparus auratus. | fish scales are an important reservoir of calcium and phosphorus and together with the skin function as an integrated barrier against environmental changes and external aggressors. histological studies have revealed that the skin and scales regenerate rapidly in fish when they are lost or damaged. in the present manuscript the histological and molecular changes underlying skin and scale regeneration in fed and fasted sea bream (sparus auratus) were studied using a microarray 3 and 7 days after s ... | 2011 | 21981800 |
| a microsatellite linkage map of striped bass (morone saxatilis) reveals conserved synteny with the three-spined stickleback (gasterosteus aculeatus). | the striped bass (morone saxatilis) and its relatives (genus morone) are of great importance to fisheries and aquaculture in north america. as part of a collaborative effort to employ molecular genetics technologies in striped bass breeding programs, we previously developed nearly 500 microsatellite markers. the objectives of this study were to construct a microsatellite linkage map of striped bass and to examine conserved synteny between striped bass and three-spined stickleback (gasterosteus a ... | 2012 | 21968826 |
| effects of waterborne cu exposure in gilthead sea bream (sparus aurata): a proteomic approach. | aquatic organisms may suffer from exposure to high cu concentrations, since this metal is widely used in feed supplementation, in pesticide formulation and as antifouling. chronic exposure to cu, even at sub-lethal doses, may strongly affect fish physiology. to date, several biomarkers have been used to detect cu exposure in fish producing contrasting results. therefore, we used a proteomic approach to clarify how cu exposure may affect the serum proteome of gilthead sea bream (sparus aurata), s ... | 2011 | 21925607 |
| stable isotope analysis combined with metabolic indices discriminates between gilthead sea bream (sparus aurata) fingerlings produced in various hatcheries. | there are few traceability systems other than genetic markers capable of distinguishing between sea products of different origin and quality. here, we address the potential of stable isotopes combined with metabolic and growth parameters as a discriminatory tool for the selection of fish seeds with high growth capacity. for this purpose, sea bream fingerlings produced in three hatcheries (spanish mediterranean coast, mc; cantabrian coast, cc; and south-iberian atlantic coast, ac) were subjected ... | 2011 | 21838305 |
| proteomics in the liver of gilthead sea bream (sparus aurata) to elucidate the cellular response induced by the intake of maslinic acid. | maslinic acid (ma) is a pentacyclic triterpene used as a feed additive to stimulate growth, protein-turnover rates, and hyperplasia in fish. to further our understanding of cellular mechanisms underlying the action of ma, we have used 2-de coupled with ms to identify proteins differentially expressed in the livers of juvenile gilthead sea bream (sparus aurata) grown under fish-farm conditions and fed with a 100 mg/kg ma-enriched diet (ma(100)). after the comparison of the protein profiles from m ... | 2011 | 21751345 |
| species specificity in the magnitude and duration of the acute stress response in mediterranean marine fish in culture. | the aim of the present study was to examine the species-specific stress response for seven mediterranean fishes in culture. also, to evaluate the method of measuring free cortisol concentration in the rearing water as a non-invasive and reliable indicator of stress in marine species, of aquaculture importance. gilthead sea bream, sparus aurata (sparidae); common dentex, dentex dentex (sparidae); common pandora, pagellus erythrinus (sparidae); sharpsnout sea bream, diplodus puntazzo (sparidae); d ... | 2011 | 21712040 |
| differential expression of pthrp and its receptor in pituitary gland and gills in estradiol-treated gilthead sea bream (sparus auratus, l.). | in the gilthead sea bream (sparus auratus) 17β-estradiol (e₂) plays an important role in the synthesis of vitellogenin. during vitellogenesis, vitellogenin as a nutritional precursor protein is loaded with calcium, which requires elevated plasma calcium levels. this is accomplished via e₂-dependent processes. reports have shown that hypercalcemic effects of e₂ are possibly mediated by another hypercalcemic factor, viz. parathyroid hormone related protein (pthrp). to further investigate the possi ... | 2011 | 21658390 |
| biological effects of marine contaminated sediments on sparus aurata juveniles. | chemical analysis of the compounds present in sediment, although informative, often is not indicative of the downstream biological effects that these contaminants exert on resident aquatic organisms. more direct molecular methods are needed to determine if marine life is affected by exposure to sediments. in this study, we used an aquatic multi-species microarray and q-pcr to investigate the effects on gene expression in juvenile sea bream (sparus aurata) of two contaminated sediments defined as ... | 2011 | 21658360 |
| aquaporin inhibition changes protein phosphorylation pattern following sperm motility activation in fish. | our previous studies demonstrated that osmolality is the key signal in sperm motility activation in sparus aurata spermatozoa. in particular, we have proposed that the hyper-osmotic shock triggers water efflux from spermatozoa via aquaporins. this water efflux determines the cell volume reduction and, in turn, the rise in the intracellular concentration of ions. this increase could lead to the activation of adenylyl cyclase and of the camp-signaling pathway, causing the phosphorylation of sperm ... | 2011 | 21620454 |
| in silico mining and characterization of simple sequence repeats from gilthead sea bream (sparus aurata) expressed sequence tags (est-ssrs); pcr amplification, polymorphism evaluation and multiplexing and cross-species assays. | we screened for simple sequence repeats (ssrs) found in ests derived from an est-database development project ('marine genomics europe' network of excellence). different motifs of di-, tri-, tetra-, penta- and hexanucleotide ssrs were evaluated for variation in length and position in the expressed sequences, relative abundance and distribution in gilthead sea bream (sparus aurata). we found 899 ests that harbor 997 ssrs (4.94%). on average, one ssr was found per 2.95 kb of est sequence and the d ... | 2011 | 21620329 |
| hepatocyte nuclear factor 4α transactivates the mitochondrial alanine aminotransferase gene in the kidney of sparus aurata. | alanine aminotransferase (alt) plays an important role in amino acid metabolism and gluconeogenesis. the preference of carnivorous fish for protein amino acids instead of carbohydrates as a source of energy lead us to study the transcriptional regulation of the mitochondrial alt (malt) gene and to characterize the enzyme kinetics and modulation of malt expression in the kidney of gilthead sea bream (sparus aurata) under different nutritional and hormonal conditions. 5'-deletion analysis of malt ... | 2012 | 21607544 |
| mast cells in the intestine and gills of the sea bream, sparus aurata, exposed to a polychlorinated biphenyl, pcb 126. | the presence of mast cells has been reported in all classes of vertebrates, including many teleost fish families. the mast cells of teleosts, both morphologically and functionally, show a close similarity to the mast cells of mammals. mast cells of teleosts, localized in the vicinity of blood vessels of the intestine, gills and skin, may play an important role in the mechanisms of inflammatory response, because they express a number of functional proteins, including piscidins, which are antimicr ... | 2012 | 21565388 |
| functional divergences of gapdh isoforms during early development in two perciform fish species. | glyceraldehyde-3-phospate dehydrogenase (gapdh) is involved in basic cell catabolic processes and, as it is thought to be continuously expressed, belongs to the group of housekeeping genes. thus, it is frequently used as an internal control in quantitative gene expression studies. however, the evidence of different expression patterns in a broad range of organisms and tissues, as well as the occurrence of different isoforms, shows that gapdh has to be reevaluated as an internal control in qpcr s ... | 2011 | 21559871 |
| cadmium accumulation and antioxidant responses in sparus aurata exposed to waterborne cadmium. | cadmium (cd), a nonessential trace element, is rapidly accumulated by most living organisms and subsequently exerts its toxicity at different molecular levels. this study exposed gilthead sea bream (sparus aurata) to waterborne 0.1 mg/l cd for 11 days and investigated the cd accumulation pattern, lipid oxidation, and response of antioxidant defences. at the end of the experiment, mean cd concentrations in gills and liver, the organs most prone to metal accumulation, were 209.4 and 371.7 ng/g ww, ... | 2012 | 21553241 |
| collagen fibrils in cultured and wild sea bream (sparus aurata) liver. an electron microscopy and image analysis study. | this study aims to measure liver collagen fibril diameter in cultured and wild sea breams (sparus aurata). cultured sea breams were fed three isonitrogenous diets. the organically produced feed contained sustainable certified fish meal (45%), fish oil (14%), and organic certified wheat; the laboratory feed contained fish meal (45%), fish oil (14%), wheat meal, and soya meal; and the commercial feed included fish meal (46%), fish oil (17%), soya meal, wheat meal, and corn gluten meal. the organic ... | 2011 | 21516288 |
| copper induces cu-atpase atp7a mrna in a fish cell line, saf1. | copper transporting atpase, atp7a, is an atp dependent copper pump present in all vertebrates, critical for the maintenance of intracellular and whole body copper homeostasis. effects of copper treatment on atp7a gene expression in fibroblast cells (saf1) of the sea bream (sparus aurata) were investigated by qrt-pcr and by a medium density microarray from a closely related species, striped sea bream (lithognathus mormyrus). to discriminate between the effects of cu and other metals, saf1 cells w ... | 2011 | 21473932 |
| feed restriction up-regulates uncoupling protein 3 (ucp3) gene expression in heart and red muscle tissues of gilthead sea bream (sparus aurata l.) new insights in substrate oxidation and energy expenditure. | the physiological regulation of the mitochondrial uncoupling protein 3 (ucp3) remains practically unexplored in fish and the aim of this study was to examine the effects of ration size on the regulation of ucp3 in heart, red skeletal muscle and white skeletal muscle of gilthead sea bream (sparus aurata l.). juvenile fish were fed at three different levels for 11 weeks: i) full ration until visual satiety (r(100) group), ii) 70% of satiation (r(70) group) and iii) 70% of satiation with two finish ... | 2011 | 21463702 |
| cellular responses in the skin of the gilthead sea bream sparus aurata l. and the sea bass dicentrarchus labrax (l.) exposed to high ammonia. | adult gilthead sea bream sparus aurata and sea bass dicentrarchus labrax were exposed for 24 and 48 h, respectively, to two concentrations of ammonia each (mean values of 3·34 and 13·10 mg l(-1) ta-n in s. aurata; 2·99 and 11·90 mg l(-1) ta-n in d. labrax). light microscopy and computerized morphometry were used to evaluate ammonia-induced alterations in skin structure during exposure and following recovery in normal water. in s. aurata, ammonia exposure induced a concentration-dependent increas ... | 2011 | 21463312 |
| insulin and igf-i effects on the proliferation of an osteoblast primary culture from sea bream (sparus aurata). | bone deformities in several fish species, like gilthead sea bream (sparus aurata), are currently a major problem in aquaculture. to gain knowledge of fish skeletal development, a primary cell culture has been established from sea bream vertebra. the initial fibroblastic phenotype of the cells changed to a polygonal shape during the culture, and the addition of an osteogenic medium promoted the deposition of minerals in the extracellular matrix. cell proliferation was analyzed using the mtt assay ... | 2011 | 21447336 |
| mast cell responses to ergasilus (copepoda), a gill ectoparasite of sea bream. | immunocytochemical, light microscopy and ultrastructural studies were conducted on gill of sea bream, sparus aurata l., naturally parasitized with the important parasitic copepod ergasilus sp. to assess pathology and cellular responses. thirty-seven s. aurata were examined from a fish farm; 26 (70%) were parasitized, with infection intensity ranging from 3 to 55 parasites per fish. hosts were divided into two groups, lightly infected fish (<15 parasites per fish) and heavily infected fish (>15 p ... | 2011 | 21316458 |
| quantitative trait loci involved in sex determination and body growth in the gilthead sea bream (sparus aurata l.) through targeted genome scan. | among vertebrates, teleost fish exhibit a considerably wide range of sex determination patterns that may be influenced by extrinsic parameters. however even for model fish species like the zebrafish danio rerio the precise mechanisms involved in primary sex determination have not been studied extensively. the zebrafish, a gonochoristic species, is lacking discernible sex chromosomes and the sex of juvenile fish is difficult to determine. sequential protandrous hermaphrodite species provide disti ... | 2011 | 21304996 |
| cellular morphology and markers of cartilage and bone in the marine teleost sparus auratus. | modifications have been characterised in terms of cellular organisation and the extracellular matrix (ecm) during bone ontogeny in the sea bream (sparus auratus). during endochondral development, the agglomeration of matrix-secreting cells gives rise to chondrones; these chondrones frequently contain proliferating-cell-nuclear-antigen-positive cells, which subsequently become large collagen-ii-positive cells with the characteristics of chondrocytes. moreover, the matrix:cell ratio within the per ... | 2011 | 21234603 |
| annual variations of the plasmatic levels of glucose and amino acid and daily changes under different natural conditions of temperature and photoperiod in gilthead sea bream (sparus aurata, l.). | daily and annual changes in the plasmatic glucose and amino acid concentration have been determined in sparus aurata l. fish (average weight 330 g) were kept in cages under natural conditions of temperature and photoperiod and fed with a commercial diet. the months studied were chosen to establish whether there is any influence on the plasmatic glucose and amino acid concentration due to the change in temperature and photoperiod (equal photoperiod and different temperature, march and october; di ... | 2011 | 21174148 |
| recovery of opercular anomalies in gilthead sea bream, sparus aurata l.: morphological and morphometric analysis. | opercular anomalies are very frequent in reared gilthead sea bream and these can negatively influence the product value. field observations have suggested that opercular malformations can recover over time. in order to verify this hypothesis, 140-day-old gilthead sea bream with monolateral opercular anomalies were divided into three groups, according to the type and increasing seriousness of the opercular malformations, and another group was composed of fish with bilateral opercular anomalies. a ... | 2011 | 21166823 |
| divergence of duplicate pomc genes in gilthead sea bream sparus auratus. | proopiomelanocorticotrophin (pomc) in vertebrates is produced in the pituitary gland and undergoes post-translational processing to give rise to a range of biologically active peptides. teleosts possess 2-3 different pomc transcripts which have been proposed to have originated from a whole or partial genome duplication. in the present study 2 transcripts of gilthead sea bream pomc (sbpomc-α1 and α2) were cloned and characterised. sbpomc-α1 is expressed principally in the melanotroph cells of the ... | 2011 | 21147111 |
| changes in adipocyte cell size, gene expression of lipid metabolism markers, and lipolytic responses induced by dietary fish oil replacement in gilthead sea bream (sparus aurata l.). | the effects of fish oil (fo) substitution by 66% vegetable oils in a diet with already 75% vegetable protein (66vo) on adipose tissue lipid metabolism of gilthead sea bream were analysed after a 14-month feeding trial. in the last 3 months of the experiment, a fo diet was administrated to a 66vo group (group 66vo/fo) as a finishing diet. hormone-sensitive lipase (hsl) activity was measured in adipose tissue and adipocyte size, and hsl, lipoprotein lipase and liver x receptor gene expression in i ... | 2011 | 21130894 |
| patterns of gastric evacuation, digesta characteristics and ph changes along the gastrointestinal tract of gilthead sea bream (sparus aurata l.) and european sea bass (dicentrarchus labrax l.). | a comparative study of gastric evacuation rates (gers) and digesta content, moisture and ph values along the gastrointestinal tract was performed between gilthead sea bream and european sea bass. in order to distinguish species-specific differences from diet-elicited effects, all parameters were determined under either a fishmeal diet or a carob seed germ meal diet that contained high levels of total and soluble non-starch polysaccharides. gers were significantly different between species and th ... | 2011 | 21130892 |
| a comparative bac map for the gilthead sea bream (sparus aurata l.). | this study presents the first comparative bac map of the gilthead sea bream (sparus aurata), a highly valuated marine aquaculture fish species in the mediterranean. high-throughput end sequencing of a bac library yielded 92,468 reads (60.6 mbp). comparative mapping was achieved by anchoring bac end sequences to the three-spined stickleback (gasterosteus aculeatus) genome. bacs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. a fraction of 5,249 bacs ... | 2011 | 21049003 |
| metabolic and osmoregulatory changes and cell proliferation in gilthead sea bream (sparus aurata) exposed to cadmium. | the impact of cadmium on metabolism and osmoregulation was assessed in gilthead sea bream (sparus aurata). seawater acclimated fish were injected intraperitoneally with a sublethal dose of cadmium (1.25 mg cd/kg body wt). after 7 days, half of the injected fish were sampled. the remaining fish were transferred to hypersaline water and sampled 4 days later. gill and kidney na(+)/k(+)-atpase activities, plasma levels of cortisol, several metabolites and osmolytes, as well as osmolality were measur ... | 2011 | 20933284 |
| physiological stress and innate immune response in gilthead sea bream (sparus aurata) and sea bass (dicentrarchus labrax) exposed to combination of trimethoprim and sulfamethoxazole (tmp-smx). | the purpose of this study was to determine whether gilthead sea bream and sea bass treated with combination of trimethoprim and sulfamethoxazole (tmp-smx) differed in terms of physiological and innate immune biomarkers. fish were exposed to tmp-smx at 40 ppm concentration for 1 h as a prophylactic usage. plasma cortisol, glucose, electrolytes (ca, p, na, k, cl, and mg) as well as plasma lysozyme activity, c-reactive protein (crp), and ceruloplasmin (cp) were measured soon after treatment and fol ... | 2011 | 20924669 |
| a comparative study on the composition and importance of free amino acids in semen of gilthead sea bream, sparus aurata, and perch, perca fluviatilis. | a comparative study was conducted on the free amino acid composition of gilthead sea bream, sparus aurata, and perch, perca fluviatilis. also the effect of 21 free amino acids on sperm motility was investigated. spermatozoa were incubated in species-specific motility-inhibiting saline solution containing the different amino acids for 48 h. thereafter, the motility was activated and investigated using computer-assisted cell motility analysis. twelve free amino acids, respectively, were detected i ... | 2010 | 20924668 |
| cellular response in semi-intensively cultured sea bream gills to ergasilus sieboldi (copepoda) with emphasis on the distribution, histochemistry and fine structure of mucous cells. | light and ultrastructure studies were carried out on gill of sea bream, sparus aurata l., naturally infected with ergasilus sieboldi (copepoda) to assess pathology and host cell responses. thirty s. aurata were examined, and 23 (74%) were infected, the intensity of infection ranging from 3 to 50 parasites per host. the copepod encircled gill lamellae with its second antennae, occluded arteries, compressed the epithelium, provoked hyperplasia and haemorrhage, and often caused tissue disruption. a ... | 2010 | 20850928 |
| adrenocorticotrophic hormone-stimulated cortisol release by the head kidney inter-renal tissue from sea bream (sparus aurata) fed with linseed oil and soyabean oil. | the mode of action of highly unsaturated fatty acids (hufa) in regulating gilthead sea bream (sparus aurata) head kidney (hk) cortisol production was studied through in vitro trials using a dynamic superfusion system. fish were previously fed with different diets containing several inclusion levels of linseed oil (lo) or soyabean oil (so) for 26 weeks. five diets were tested; anchovy oil was the only lipid source for the control diet (fish oil, fo) and two different substitution levels (70 and 1 ... | 2011 | 20836902 |
| melatonin concentrations during larval and postlarval development of gilthead sea bream sparus auratus: more than a time-keeping molecule? | in this study, melatonin (mel) and thyroxine (t(4)) concentrations were measured during larval and postlarval development of gilthead sea bream sparus auratus hormones were measured in whole bodies of larvae or the head and trunk of postlarvae after 67 days of exposure to constant light, 24l:0d, constant darkness, 0l:24d or 12l:12d and in the plasma of 6 month juveniles kept under the 12l:12d, 0l:24d and 24l:0d regimes. high mel concentrations in larvae suggested a distinct role of mel in early ... | 2009 | 20738488 |
| growth/differentiation factor-11: an evolutionary conserved growth factor in vertebrates. | growth and differentiation factor-11 (gdf-11) is a member of the transforming growth factor-β superfamily and is thought to be derived together with myostatin (known also as gdf-8) from an ancestral gene. in the present study, we report the isolation and characterization of gdf-11 homolog from a marine teleost, the gilthead sea bream sparus aurata, and show that this growth factor is highly conserved throughout vertebrates. using bioinformatics, we identified gdf-11 in tetraodon, takifugu, medak ... | 2010 | 20694476 |