| complete genome sequence of streptococcus agalactiae zq0910, a pathogen causing meningoencephalitis in the gift strain of nile tilapia (oreochromis niloticus). | streptococcus agalactiae (group b streptococcus [gbs]) is a pathogen that causes meningoencephalitis in nile tilapia (oreochromis niloticus). here, we reported the complete genome sequence of s. agalactiae strain zq0910, which was isolated from the gift strain of nile tilapia in guangdong, china. | 2012 | 22933761 |
| construction of a streptococcus iniae sortase a mutant and evaluation of its potential as an attenuated modified live vaccine in nile tilapia (oreochromis niloticus). | streptococcus iniae is a major gram-positive aquatic pathogen, which causes invasive diseases in cultured fish worldwide. the identification of potential virulence determinants of streptococcal infections will help to understand and control this disease, but only a few have been confirmed in s. iniae. sortase a (srta) is the key enzyme that anchors pre-mature cell wall-attached proteins to peptidoglycan and it can affect the correct positioning of surface proteins, as well as the course of gram- ... | 2014 | 25090938 |
| toxicity and genotoxicity of wastewater from gasoline stations. | the toxicity and genotoxicity of wastewater from eight gasoline stations in brasília, brazil's capital city, was studied by assessing chromosomal aberrations, chromosomal malsegregation and the mitotic index in alliumcepa root cells, and the occurrence of micronucleus and nuclear abnormalities in peripheral erythrocytes of tilapia fish (oreochromis niloticus). the content of gasoline station effluents was also analyzed based on several physico-chemical parameters. none of the wastewater samples ... | 2009 | 21637464 |
| detoxification and antioxidant effects of garlic and curcumin in oreochromis niloticus injected with aflatoxin b₁ with reference to gene expression of glutathione peroxidase (gpx) by rt-pcr. | the present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and gpx gene expression against aflatoxicosis of o. niloticus. in total, 180 of tilapia were divided into ten groups; t1 represented the negative control fed on a basal diet, and t2 was injected with a single intraperitoneal (i.p.) dose of afb1 (6 mg/kg b.w.). fish in t3-t6 were fed on a basal diet supple ... | 2016 | 26590820 |
| molecular cloning, sequence identification, and gene expression analysis of bovine adcy2 gene. | adenylyl cyclase 2 (adcy2), a class b member of adenylyl cyclases, is important in accelerating phosphor-acidification as well as glycogen synthesis and breakdown. given its distinct role in flesh tenderization after butchering, we cloned and sequenced the adcy2 gene from yanbian cattle and assessed its expression in bovine tissues. a 2947 bp nucleotide sequence representing the full-length cdna of bovine adcy2 gene was obtained by 5' and 3' remote analysis computations for gene expression. anal ... | 2014 | 24797538 |
| antagonistic activity of dietary allicin against deltamethrin-induced oxidative damage in freshwater nile tilapia; oreochromis niloticus. | allicin, the main biologically active component of garlic clove extracts, has been evaluated for its' efficacy in preventing deltamethrin-induced oxidative damage in nile tilapia; oreochromis niloticus. fish were fed on 2 different doses of 0.5 g and 1 g of allicin/kg diet for 28 days. serum aspartate aminotransferase (ast), alanine aminotransferase (alt), alkaline phosphatase (alp), cholesterol, urea, uric acid, creatinine, total protein, albumin and globulin were estimated. moreover, the level ... | 2015 | 25450927 |
| lrp13 is a novel vertebrate lipoprotein receptor that binds vitellogenins in teleost fishes. | transcripts encoding a novel member of the lipoprotein receptor superfamily, termed ldl receptor-related protein (lrp)13, were sequenced from striped bass (morone saxatilis) and white perch (morone americana) ovaries. receptor proteins were purified from perch ovary membranes by protein-affinity chromatography employing an immobilized mixture of vitellogenins aa and ab. rt-pcr revealed lrp13 to be predominantly expressed in striped bass ovary, and in situ hybridization detected lrp13 transcripts ... | 2014 | 25217480 |
| biomagnification of ddt and its metabolites in four fish species of a tropical lake. | the concentrations and biomagnifications of dichlorodiphenyltrichloroethane (ddt) and its metabolites were examined in four fish species (clarias gariepinus, oreochromis niloticus, tilapia zillii, and carassius auratus) from lake ziway, rift valley, ethiopia. paired stomach content analysis, and stable isotope ratio of nitrogen (δ(15)n, ‰) and carbon (δ(13)c, ‰) were used to study the trophic position of the fish species in the lake. 4,4'-dde, 4,4'-ddt and 4,4'-ddd were the main ddts identified ... | 2013 | 23790590 |
| partial sodium replacement in tilapia steak without loss of acceptability. | the aim of this study was to evaluate physical and sensory characteristics of low-sodium tilapia steaks restructured with microbial transglutaminase. polyphosphate (0.4%), condiment mixes-onion powder and garlic powder (2.5%), water (10.0%), transglutaminase (1.0%), and different types of salts (1.5%) were mixed with non-commercial sized fillets. substitution of nacl by kcl or mgcl2 (at 50% each) affected quality attributes and decreased (p < 0.05) consumer acceptability. the tri-salt steaks (kc ... | 2015 | 24831644 |
| trials for the control of trichodinosis and gyrodactylosis in hatchery reared oreochromis niloticus fries by using garlic. | the present work was designed to study the prevalence of trichodinosis and gyrodactylosis in oreochromis niloticus fries, and to test the therapeutic efficacy and preventive efficacy of garlic oil and crushed garlic cloves. trichodinosis and gyrodactylosis are ectoparasitic diseases that affect most warm freshwater fish, especially fries and fingerlings. in a private o. niloticus fish hatchery, the prevalence of trichodinosis in 5-, 15- and 30-day-old-fries was 37%, 23% and 40.5%, respectively. ... | 2012 | 22137346 |
| expression characterization and promoter activity analysis of the tilapia (oreochromis niloticus) myosin light chain 3 promoter in skeletal muscle of fish. | a tilapia (oreochromis niloticus) myosin light chain 3 (mlc3) promoter region (~4.3 kb) was isolated and characterized. sequence analysis of the clone revealed high similarity with a tilapia gene encoding the mlc3 promoter region, exon 1, and intron 1. the clone contained several putative binding sequences for transcription factors, including mef-2, myog, myod, pknox1, and areb6. deletion of a region of the tilapia mlc3 promoter (801 to -3,881 bp) enhanced promoter activity, as determined by dir ... | 2014 | 24146265 |
| observations on the biology of rhabdochona kidderi texensis, a parasite of north american cichlids. | an examination of a sample of benthic invertebrates collected from the upper san marcos river in southwestern texas, usa in september 1999 revealed that the nymph of the ephemeropteran tricorythodes curvatus served as natural intermediate host of the nematode rhabdochona kidderi texensis (nematoda: rhabdochonidae), an intestinal parasite mainly of the rio grande perch (cichlasoma cyanoguttatum) in this locality; the prevalence of the parasite's third- and fourth-stage larvae in mayflies was 6.8% ... | 2001 | 11520446 |
| experimental infection of aphanomyces invadans and susceptibility in seven species of tropical fish. | epizootic ulcerative syndrome (eus) causes by aquatic oomycete fungus, aphanomyces invadans is a dangerous fish disease of a wide range of fresh and brackish water, wild and farmed fish throughout the world. the objective of the present study was to determine the susceptibility of a number of tropical fish species to the eus and compare the severity of infection between experimental groups. | 2015 | 27047195 |
| ddt and derivatives in indicator species of the aquatic food web of rangsit agricultural area, central thailand. | the presence of ddt and derivatives in the food web of freshwater ecosystems of rangsit agricultural area, pathum thani province, thailand were investigated from june 2004 to may 2007. by using gas chromatography (gc) with micro electron capture detector (mu ecd), ddt and derivatives in water, sediment, and fifteen indicator species i.e., 2 producers; eichhornia crassipes and plankton (phyto- and zoo- plankton), an herbivore; trichogaster microlepis (3) 3 omnivores; trichogaster trichopterus, or ... | 2009 | 20161116 |
| seawater acclimation and inositol monophosphatase isoform expression in the european eel (anguilla anguilla) and nile tilapia (orechromis niloticus). | inositol monophosphatase (impa) is responsible for the synthesis of inositol, a polyol that can function as an intracellular osmolyte helping re-establish cell volume when exposed to hypertonic environments. some epithelial tissues in euryhaline teleosts such as the eel and tilapia encounter considerable hyperosmotic challenge when fish move from freshwater (fw) to seawater (sw) environments; however, the roles played by organic osmolytes, such as inositol, have yet to be determined. syntenic an ... | 2013 | 23739342 |
| steroidogenic response of carp ovaries to piscine fsh and lh depends on the reproductive phase. | the gonadotropins (gths) follicle-stimulating hormone (fsh) and luteinizing hormone (lh) are the key regulators of reproduction. we determined the competence of heterologous recombinant gths at eliciting steroid secretion from carp ovaries at different reproductive stages. we collected carp ovaries at: early, mid and end vitellogenesis, when most of the oocytes still contained a germinal vesicle (gv) at a central stage, and mature ovaries with a migrating gv. plasma estradiol (e2) levels at earl ... | 2012 | 22522050 |
| sequence and expression of androgen receptor and estrogen receptor gene in the sex types of protogynous wrasse, halichoeres trimaculatus. | sex steroid hormones play important roles in sex change and behavior of wrasses. their actions are considered to be mediated through the nuclear hormone receptors. in this study, to elucidate the roles of estrogen receptor (er) and androgen receptor (ar) in the reproduction of the protogynous wrasse, halichoeres trimaculatus, ar and er genes were partially cloned using 5'- and 3'-race and their transcript levels in the gonads and the brain were measured by competitive rt-pcr. the amino acid sequ ... | 2002 | 12383444 |
| trends in the evolution of the prodynorphin gene in teleosts: cloning of eel and tilapia prodynorphin cdnas. | the detection of the prodynorphin gene in anuran amphibians and lungfishes may indicate that this gene arose as a result of the duplication of the proenkephalin gene early during the divergence of the sarcopterygii, or that this gene may predate the divergence of the ray-finned fish and the lobe-finned fish. the cloning of prodynorphin-related genes from the pufferfish and zebrafish supports the latter hypothesis. this study analyzes trends in the radiation of the prodynorphin gene in teleosts. ... | 2006 | 16274850 |
| identification and characterization of rab7 from orange-spotted grouper, epinephelus coioides. | rab7 is a small gtpase that regulates vesicular traffic from early to late endosomal stages of the endocytic pathway. during the virus-host co-evolution, host rab7 was also exploited by virus to complete their life cycle. to date, however, the roles of fish rab7 in virus infection remained largely unknown. here, we cloned and characterized a rab7 gene from grouper, epinephelus coioides (ec-rab7). the full-length ec-rab7 cdna was composed of 1182 bp and encoded a polypeptide of 207 amino acids wh ... | 2014 | 24161772 |
| modulation of genotoxicity and endocrine disruptive effects of malathion by dietary honeybee pollen and propolis in nile tilapia (oreochromis niloticus). | the present study aimed at verifying the usefulness of dietary 2.5% bee-pollen (bp) or propolis (prop) to overcome the genotoxic and endocrine disruptive effects of malathion polluted water in oreochromis niloticus (o. niloticus). the acute toxicity test was conducted in o. niloticus in various concentrations (0-8 ppm); mortality rate was assessed daily for 96 h. the 96 h-lc50 was 5 ppm and therefore 1/5 of the median lethal concentration (1 ppm) was used for chronic toxicity assessment. in expe ... | 2014 | 25685536 |
| characterization of catalytic efficiency parameters of brain cholinesterases in tropical fish. | brain cholinesterases from four fish (arapaima gigas, colossoma macropomum, rachycentron canadum and oreochromis niloticus) were characterized using specific substrates and selective inhibitors. parameters of catalytic efficiency such as activation energy (ae), k(cat) and k(cat)/k(m) as well as rate enhancements produced by these enzymes were estimated by a method using crude extracts described here. despite the bche-like activity, specific substrate kinetic analysis pointed to the existence of ... | 2014 | 24980148 |
| comparative effect of pesticides on brain acetylcholinesterase in tropical fish. | monitoring of pesticides based on acetylcholinesterase (ache; ec 3.1.1.7) inhibition in vitro avoids interference of detoxification defenses and bioactivation of some of those compounds in non-target tissues. moreover, environmental temperature, age and stress are able to affect specific enzyme activities when performing in vivo studies. few comparative studies have investigated the inter-specific differences in ache activity in fish. screening studies allow choosing the suitable species as sour ... | 2012 | 23137979 |
| solar drying and organoleptic characteristics of two tropical african fish species using improved low-cost solar driers. | this study was done to evaluate the drying performance, efficiency, and effectiveness of five different types of improved low-cost solar driers in terms of moisture loss from two tropical african fish species clarias gariepinus (african sharp tooth catfish) and oreochromis niloticus (nile tilapia) and testing the organoleptic characteristics of the dried samples. the driers used were made from plastic, aluminum, glass, glass with black igneous stone, and mosquito net, with traditional direct ope ... | 2014 | 24936294 |
| concentrations and human health risk assessment of organochlorine pesticides in edible fish species from a rift valley lake-lake ziway, ethiopia. | fish consumption is known to have several health benefits for humans. however, the accumulation of organic pollutants, like organochlorine pesticides (ocps) could pose health hazards. thus, ocps in edible fish species (oreochromis niloticus, tilapia zillii, carassius spp., and clarias gariepinus) from lake ziway, an ethiopian rift valley lake were investigated to assess the potential human health hazards of these contaminants. dichlorodiphenyltrichloroethanes (ddts), hexachlorocyclohexanes (hchs ... | 2014 | 24836883 |
| tissue metal distribution and risk assessment for important fish species from saudi arabia. | the concentrations of nine heavy metals (cr, cd, pb, ni, cu, zn, mn, as and fe) in the muscle tissue of four fish species (oreochromis niloticus, clarias gariepinus, poecilia latipinna and aphanius dispar dispar) collected from wadi hanifah were detected in two different seasons. the concentrations of the studied heavy metals, except for cd, pb, ni and cu in a. d. dispar and p. latipinna, were found to be below the safe limits recommended by various authorities and thus provide an indication of ... | 2014 | 24201710 |
| localization of acid phosphatase activity in the testis of two teleostean species (oreochromis niloticus and odonthestes perugiae). | acid phosphatase (acp) activity was investigated in the testes of two species of teleosts in two seasons: summer and winter. acp activity was detected in sertoli cells from tilapia (oreochromis niloticus) only during the nonreproductive period of its annual cycle, corresponding to the winter months. in kingfish (odonthestes perugiae), the enzymatic reaction was identified during the non-reproductive period (summer) in epithelial cells of the efferent ducts but not in sertoli cells. these data su ... | 2004 | 15744426 |
| maturation profile and fecundity of the exotic oreochromis niloticus in the river yamuna, india. | the knowledge on the reproductive biology of fish oreochromis niloticus (linnaeus, 1758) in riverine condition is meagre in the indian sub-continent which is necessary for the development of an appropriate fishery management protocols for the rivers. in this context, the gonadal maturation profile and fecundity of 0. niloticus was studied. mature, spawning and spent females recorded in almost all the months of the year evidenced multiple and prolonged breeding behaviour. an overall sex ratio (m/ ... | 2015 | 26364471 |
| [ichthyofauna from wetlands of san pedro, balancán, tabasco, méxico]. | san pedro river's wetlands sustain trophic nets in the fluvial system, due to the high habitat availability, and space and temporal variations. in order to describe the relationship between environmental parameters and ichthyofauna, this study evaluated fish assemblages composition, distribution, abundance, density, biomass, richness species, diversity and equitability in the wetlands. sampling considered three different sites and climatic seasons (dry, rainy and cold fronts). the physical and c ... | 2011 | 21721234 |
| identification of novel and zoonotic cryptosporidium species in fish from papua new guinea. | there is still limited information on the distribution of cryptosporidium species and genotypes in fish. the present study investigated the prevalence of cryptosporidium species in cultured freshwater (n=132), wild freshwater (n=206) and wild marine (n=276) fish in papua new guinea (png) by pcr screening at the 18s rrna locus. a total of seven fish (2 cultured freshwater, 1 wild freshwater and 4 wild marine fish) were identified as positive for cryptosporidium. specifically, cryptosporidium was ... | 2013 | 24064001 |
| studies on the effect of pollution on lake manzala ecosystem in port-said, damietta and dakahlia governorates egypt. | this work studied how pollution impacts the ecosystem of lake manzala by determination of physicochemical parameters, studying biodiversity of aquatic plants and macroinvertebrates, and determining bioaccumulation of pb, cu, cd & zn in some major organisms, biomphalaria alexandrina and melanoides tuberculata snails and oreochromis niloticus fish. the more near to mediterranean sea and to the industrial area, port-said and damietta sites showed higher dissolved oxygen and conductivity than dakahl ... | 2015 | 26012230 |
| impact of certain plants and synthetic molluscicides on some fresh water snails and fish. | the lc50 (78, 85 ppm) and lc90 (88, 135 ppm) of anagalis arvensis and calendula micrantha respectively against biomphalaria alexandrina were higher than those of the non-target snails, physa acuta, planorbis planorbis, helisoma duryi and melanoides tuberculata. in contrast, the lc50 of niclosamide (0.11 ppm) and copper sulphate (cuso4) (0.42 ppm) against b. alexandrina were lower than those of the non-target snails. the mortalities percentage among non-target snails ranged between 0.0 & 20% when ... | 2005 | 16333905 |
| establishment and growth responses of nile tilapia embryonic stem-like cell lines under feeder-free condition. | embryonic stem (es) cells provide an invaluable tool for molecular analysis of vertebrate development and a bridge linking genomic manipulations in vitro and functional analysis of target genes in vivo. work towards fish es cells so far has focused on zebrafish (danio renio) and medaka (oryzias latipes). here we describe the derivation, pluripotency, differentiation and growth responses of es cell lines from nile tilapia (oreochromis niloticus), a world-wide commercial farmed fish. these cell li ... | 2017 | 28230233 |
| effect of broccoli (brassica oleracea) and its phytochemical sulforaphane in balanced diets on the detoxification enzymes levels of tilapia (oreochromis niloticus) exposed to a carcinogenic and mutagenic pollutant. | tilapia fish (oreochromis niloticus) were fed with enriched diets containing broccoli and its phytochemical sulforaphane over 30 d. the levels of cytochrome p450, superoxide dismutase, catalase, lipid peroxidation and glutathione-s-transferase activities were measured. basal value of cytochrome p450 activity was significantly increased as consequence of the broccoli and sulforaphane enriched diets, while no statistically significant changes were found on catalase and lipid peroxidation activitie ... | 2009 | 19144376 |
| mercury concentrations are low in commercial fish species of lake ziway, ethiopia, but stable isotope data indicated biomagnification. | stable isotope ratios of nitrogen (δ(15)n) and carbon (δ(13)c), complemented by stomach content data, were used to assess the food web structure and trophic transfer of mercury (hg) in four commercial fish species of lake ziway, ethiopia: nile tilapia (oreochromis niloticus), catfish (clarias gariepinus), tilapia zillii, and golden carp (carassius auratus). total mercury (thg in mg kg(-1), ww) concentrations were low, with mean values of 0.033, 0.034, 0.025, and 0.011, in c. gariepinus, t. zilli ... | 2011 | 21296421 |
| acoustical stress and hearing sensitivity in fishes: does the linear threshold shift hypothesis hold water? | mammals exposed to loud aerial sounds exhibit temporary threshold shifts (tts) that are linearly related to increases of sound pressure above baseline hearing levels. it was unknown if this relationship held true for aquatic ectotherms such as fishes. to test this linear threshold shift hypothesis (lints) in fishes, we examined the effects of increased ambient sound on hearing of two species differing in hearing capabilities: goldfish (carassius auratus; a hearing specialist) and tilapia (oreoch ... | 2004 | 15339955 |
| the cardiac internuncial cell in carassius auratus longsdorffii and other 19 teleost species: a fine-structural study. | thin-section studies of the sino-auricular border region in the hearts of 20 species of teleost fish revealed the cardiac internuncial cell (cic), hitherto known only in misgurnus, to occur also in 8 species (acheilognathus lanceolatus, carassius auratus longsdorffii, cyprinus carpio, girella punctata, kareius bicoloratus, rhodeus ocellatus ocellatus, rhyncopelates oxyrhynchus, tilapia nilotica), but to be absent in the other 12 species. there was an indication that the cic was close in nature t ... | 1997 | 9412743 |
| activity of esterases from different tissues of freshwater fish and responses of their isoenzymes to inhibitors. | activity of nonspecific esterase from different tissues (i.e., liver, gallbladder, heart, intestine, and muscle) of five species of freshwater fish, namely, topmouth gudgeon (pseudorasbora parva), goldfish (carassius auratus), nile tilapia (tilapia nilotica), mosquitofish (gambusia affinis), and rainbow trout (salmo gairdneri) was tested using alpha-naphthyl acetate as substrate. the results indicated that activity of the enzyme was mainly concentrated in the digestive system (i.e., intestine, l ... | 1997 | 9176555 |
| correlation between biochemical parameters and susceptibility of freshwater fish to malathion. | acute toxicity (96-h lc50) of malathion was tested using five species of freshwater fish, namely, topmouth gudgeon (pseudorasbora parva), goldfish (carassius auratus), nile tilapia (tilapia nilotica), mosquitofish (gambusia affinis), and rainbow trout (salmo gairdneri). correlation was found between susceptibility and biochemical parameters such as activity of brain acetylcholinesterase (ache) and in vitro resistance of the enzyme to inhibition (ic50) of malaoxon (a major metabolite of malathion ... | 1996 | 8691510 |
| assessment study on the use of pawpaw; carica papaya seeds to control oreochromis niloticus breeding. | this study was carried out to assess the ability of using pawpaw (carica papaya) seeds as a natural reproduction inhibitor for tilapia fish (oreochromis niloticus) culture to control its breeding. biochemical, physiological and histopathological effects ofpawpaw seeds on male tilapia fish were also determined. mature male tilapia were stocked for 4 weeks and treated with low dose (3 g/kg/day) and high dose (6 g/kg/day) of ground dried pawpaw seeds mixed with their feed. the obtained results show ... | 2011 | 22335051 |
| comparative mapping for bighead carp (aristichthys nobilis) against model and non-model fishes provides insights into the genomic evolution of cyprinids. | comparative mapping provides an efficient method to connect genomes of non-model and model fishes. in this study, we used flanking sequences of the 659 microsatellites on a genetic map of bighead carp (aristichthys nobilis) to comprehensively study syntenic relationships between bighead carp and nine model and non-model fishes. of the five model and two food fishes with whole genome data, cyprinus carpio showed the highest rate of positive blast hits (95.3 %) with bighead carp map, followed by d ... | 2015 | 25627158 |
| respiratory characteristics of muscle fibres in a fish (chaenocephalus aceratus) that lacks haem pigments. | 1. oxygen consumption, mitochondrial content and enzyme activities were determined in identified muscle fibre types of the 'haemoglobin-less' icefish chaenocephalus aceratus lönnberg. 2. small bundles (2-12) of fast and slow fibres were isolated from the myotomal and superficial pectoral fin abductor muscles, respectively. at 0 degrees c the time to 50% peak force and the half-relaxation time of isometric twitches were, respectively, 18 +/- 1 and 38 +/- 4 ms for fast and 43 +/- 3 and 119 +/- 21 ... | 1987 | 3430116 |
| physical modification of palm kernel meal improved available carbohydrate, physicochemical properties and in vitro digestibility in economic freshwater fish. | unavailable carbohydrates are an important limiting factor for utilization of palm kernel meal (pkm) as aquafeed ingredients. the aim of this study was to improve available carbohydrate from pkm. different physical modifications including water soaking, microwave irradiation, gamma irradiation and electron beam, were investigated in relation to chemical composition, physicochemical properties and in vitro carbohydrate digestibility using digestive enzymes from economic freshwater fish. | 2013 | 23881536 |
| cyp1a andother biomarker responses to effluents from a textile mill in the volta river (ghana) using caged tilapia (oreochromis niloticus) andsediment-exposed mudfish (clarias anguillaris). | abstract the ecotoxicological effects of a textile mill effluent were investigated by caging tilapia (oreochromis niloticus) in the volta river, ghana, andby exposing mudfish (clarias anguillaris) to sediment collected from the same river. tilapia were caged for 3 weeks at three locations (0.6, 4, and8 km) downstream from the effluent outlet. mudfish were exposed in the laboratory for 2 weeks to sediment collected from the vicinity of the effluent outlet and8 km downstream. upstream reference lo ... | 1996 | 23888992 |
| genome-wide identification, evolution and expression analysis of nuclear receptor superfamily in nile tilapia, oreochromis niloticus. | the nuclear receptor (nr) superfamily, which is divided into 7 subfamilies, constitutes one of the largest classes of transcription factors. in this study, through comprehensive database search, we identified all nrs (including 4 novel members) from the tilapia (75), common carp (137), zebrafish (73), fugu (73), tetraodon (72), stickleback (70), medaka (69), coelacanth (55), spotted gar (51) and elephant shark (50). for 21 nrs, two duplicates were found in teleosts, while only one in tetrapods. ... | 2015 | 26024593 |
| poly- and perfluoroalkylated substances (pfass) in water, sediment and fish muscle tissue from lake tana, ethiopia and implications for human exposure. | lake tana is ethiopia's largest lake and there are plans to increase the harvest of fish from the lake. the objective of this study was to assess the levels of poly- and perfluoroalkyl substances (pfass) in different compartments of the lake (water, sediment, and fish muscle tissue), and its implications for human exposure. the results showed higher pfas concentrations in piscivorous fish species (labeobarbus megastoma and labeobarbus gorguari) than non-piscivorous species (labeobarbus intermedi ... | 2016 | 27665295 |
| supplemental effects of mixed ingredients and rice bran on the growth performance, survival and yield of nile tilapia, oreochromis niloticus reared in fertilized earthen ponds. | unaffordability of commercial feeds to semi-intensive nile tilapia, oreochromis niloticus farmers has led to reliance on supplemental feeding and fertilization for nutrition of their fish without a scientific basis. this study compared the growth, survival, condition factor and yield performance of o. niloticus fed on mixed ingredients (mi) and rice bran alone (rb) diets reared in fertilized earthen ponds in small scale farmers' ponds. the study also determined phytoplankton composition, biomass ... | 2016 | 26759744 |
| stock assessment of fishery target species in lake koka, ethiopia. | effective management is essential for small-scale fisheries to continue providing food and livelihoods for households, particularly in developing countries where other options are often limited. studies on the population dynamics and stock assessment on fishery target species are thus imperative to sustain their fisheries and the benefits for the society. in lake koka (ethiopia), very little is known about the vital population parameters and exploitation status of the fishery target species: til ... | 2015 | 26666131 |
| reproductive biology of female nile tilapia oreochromis niloticus (linnaeus) reared in monoculture and polyculture with african sharptooth catfish clarias gariepinus (burchell). | this study was conducted to assess the reproductive biology and early breeding behaviour of female oreochromis niloticus reared in monoculture and polyculture with clarias gariepinus in earthen ponds for 8 months. results revealed no significant difference in length at first maturity (l50) between females reared in monoculture and polyculture systems. similarly, no significant differences were detected in absolute fecundity, relative fecundity, gonado-somatic index and condition factor between t ... | 2015 | 26090322 |
| twelve myxosporean species of the family myxobolidae infecting freshwater fishes of the river nile, egypt, with the description of four novel species. | myxosporidian parasites infecting fish are very dangerous parasites causing severe damage to a large number of economically important fishes especially in aquaculture. a survey of myxosporean parasites infecting four species of fishes from the river nile in egypt is conducted. one hundred and ninety-five out of 316 fish specimens with a percentage of 61.7% were found to be naturally infected with these parasites. light microscopic examination of different tissues revealed the presence of 12 myxo ... | 2015 | 25952702 |
| multi pesticide and pcb residues in nile tilapia and catfish in assiut city, egypt. | the current study investigated the levels of multi pesticide residues in the highly consumed types of nile fish in egypt: tilapia and cat fish. a total of 50 nile tilapia (oreochromis niloticus) and 50 african catfish (clarias gariepinus) were collected from two areas in assiut city, where most industries are situated and where agricultural activities and raising of farm animals are the main activities. in the first area, elwasta, there is an electrical power station, and the second area, mankba ... | 2014 | 23911921 |
| toxicity of aqueous bark extract of the tree balanites aegyptiaca on the fish oreochromis niloticus. | the toxicity of an aqueous extract of balanites aegyptiaca on oreochromis niloticus was investigated. fry of the fish were exposed to acute and sublethal concentrations of the extract in 96-h and 56-d static bioassays, respectively. susceptibility of the extract on freshwater snail, biomphalaria pfeifferi, was tested. the lc50 of the lethal concentrations of the extract on the fish and the snails were 1.12 mg/l-1 and 2.0 mg/l-1 respectively, while the effects of the sublethal concentrations on t ... | 1993 | 8334460 |
| larvicidal activity of dregea volubilis and bombax malabaricum leaf extracts against the filarial vector culex quinquefasciatus. | to analyze the larvicidal activity of two plant leaf powder and leaf-extracts, dregea volubilis and bombax malabaricum against culex quinquefasciatus. | 2011 | 21771694 |
| parasite fauna of farmed nile tilapia (oreochromis niloticus) and african catfish (clarias gariepinus) in uganda. | an intensive parasite survey was conducted in 2008 to better understand the parasite fauna occurrence, distribution and diversity in the commercial aquaculture fish species in uganda. a total of 265 fish collected from hatcheries and grow-out systems were examined for parasites using routine parasitological techniques. the survey yielded 17 parasite species: 11 from oreochromis niloticus and ten from clarias gariepinus. four parasites-amirthalingamia macracantha, monobothrioides sp., zoogonoides ... | 2012 | 21688067 |
| analysis of environmental chemical residues in products of emerging aquaculture industry in uganda as case study for sub-saharan africa. | a study was conducted to analyse market-regulated heavy metals (lead, mercury and cadmium), organochlorine pesticides and total polychlorinated biphenyls (pcbs) in samples of 38 farmed fish comprising nile tilapia (oreochromis niloticus) (20 samples) and african catfish (clarias gariepinus) (18 samples) from ten selected fish farms in uganda. the goal of this case study was to understand the safety of aquaculture products from sub-saharan africa. lead was detected in all the 38 samples (maximum ... | 2008 | 24784813 |
| bioavailability and toxicity of dietborne copper and zinc to fish. | to date, most researchers have used dietborne metal concentrations rather than daily doses to define metal exposure and this has resulted in contradictory data within and between fish species. it has also resulted in the impression that high concentrations of dietborne cu and zn (e.g. > 900 mg kg(-1) dry diet) are relatively non-toxic to fish. we re-analyzed existing data using rations and dietborne metal concentrations and used daily dose, species and life stage to define the toxicity of dietbo ... | 2002 | 12161165 |
| clock-controlled endogenous melatonin rhythms in nile tilapia (oreochromis niloticus niloticus) and african catfish (clarias gariepinus). | the purpose of this work was to investigate the circadian melatonin system in two tropical teleost species characterized by different behavioral habits, nile tilapia (diurnal) and african catfish (nocturnal). to do so, fish were subjected to either a control photoperiod (12l:12d), continuous light (ll) or darkness (dd), or a 6l:6d photoperiod. under 12l:12d, plasma melatonin levels were typically low during the photophase and high during the scotophase in both species. interestingly, in both spe ... | 2008 | 18293148 |
| the effect of some selected pesticides on the growth and reproduction of fresh water oreochromis niloticus, chrysicthys nigrodigitatus and clarias gariepinus. | studies were carried out to determine the toxicity of some selected pesticides on fresh water fish in a tropical environment. the uptake of the pesticides lindane, pentachlorophenol (pcp), and propoxur, which are frequently used on farms, and in industries as well as by loggers and timber men on wood were studied in concrete ponds at the university of cape coast, in ghana. the fish used for the study were oreochromis niloticus, clarias gariepinus, and chrysicthys nigrodigitatus. they were obtain ... | 2007 | 17943223 |
| contaminant accumulation and biomarker responses in caged fish exposed to effluents from anthropogenic sources in the karnaphuly river, bangladesh. | the possible ecotoxicological effects of a paper and pulp mill effluent were investigated by measuring selected contaminants and biomarkers in caged nile tilapia (oreochromis niloticus) and sharptooth catfish (clarias gariepinus) from the karnaphuly river, bangladesh. fish were caged for 28 d at two upstream reference sites (2 and 4 km) and four downstream sites (3, 5, 8, and 12 km) from the effluent outlets. organochlorine contaminants and bile biomarkers were bioaccumulated to higher levels at ... | 2005 | 16152969 |
| morphological study on the gills of two species of fresh water fishes: oreochromis niloticus and clarias gariepinus. | the gross and microscopic morphology of the gills of both nile tilabia (oreochromis niloticus) and sharptooth catfish (clarias gariepinus) were described including light and scanning electron microscopy. the anatomical differences between the gill system of the two species were basically related to the geometry of the head and opercular cavities. each species had four pairs of gills which were connected in a median interbranchial septum. in addition, a fifth rudimentary gill without gill filamen ... | 2004 | 15481835 |
| introducing foreign genes into fish eggs with electroporated sperm as a carrier. | a new method has been developed for introduction of foreign genes into fish eggs. the procedure is based on the incubation of fish sperm cells suspended in dilute citrate solution with plasmid dna, followed by application of high-field-strength electrical pulses (electroporation) to increase dna binding., uptake, or both. tissue homogenates and genomic dna extracts of free swimming fry developed from eggs fertilized with treated sperm was tested to evaluate the efficiency of gene transfer. dot b ... | 2012 | 1308817 |
| fishmicrosat: a microsatellite database of commercially important fishes and shellfishes of the indian subcontinent. | microsatellite dna is one of many powerful genetic markers used for the construction of genetic linkage maps and the study of population genetics. the biological databases in public domain hold vast numbers of microsatellite sequences for many organisms including fishes. the microsatellite data available in these data sources were extracted and managed into a database that facilitates sequences analysis and browsing relevant information. the system also helps to design primer sequences for flank ... | 2013 | 24047532 |
| persistent halogenated compounds in fish from rivers in the pearl river delta, south china: geographical pattern and implications for anthropogenic effects on the environment. | three fish species, mud carp (cirrhinus molitorella), tilapia (tilapia nilotica), and plecostomus (hypostomus plecostomus), from rivers in the pearl river delta (prd) were analyzed for dichlorodiphenyltrichloroethane and its metabolites (ddts), hexachlorocyclohexanes (hchs), polychlorinated biphenyls (pcbs), polybrominated diphenyl ethers (pbdes), decabromodiphenyl ethane (dbdpe), and dechlorane plus (dp). the concentrations of ddts, hchs, pcbs, pbdes, dbdpe, and dp ranged from 380-57,000, 5.5-1 ... | 2016 | 26821261 |
| characterization of antioxidant system parameters in four freshwater fish species. | the potential use of antioxidant system parameters has gained considerable interest due to their pivotal role of detoxification mechanisms in environmental studies and culture fish point of view. fish with different ecological needs may have different antioxidant capacity and response to environmental contaminants. thus, the optimal working conditions and specific enzyme activities (vmax and km) of antioxidant system parameters (superoxide dismutase, sod; catalase, cat; glutathione peroxidase, g ... | 2016 | 26707186 |
| hazard assessment of metals in invasive fish species of the yamuna river, india in relation to bioaccumulation factor and exposure concentration for human health implications. | monitoring of heavy metals was conducted in the yamuna river considering bioaccumulation factor, exposure concentration, and human health implications which showed contamination levels of copper (cu), lead (pb), nickel (ni), and chromium (cr) and their dispersion patterns along the river. largest concentration of pb in river water was 392 μg l(-1); cu was 392 μg l(-1) at the extreme downstream, allahabad and ni was 146 μg l(-1) at midstream, agra. largest concentration of cu was 617 μg kg(-1), n ... | 2014 | 24526612 |
| cadmium accumulation in gill, liver, kidney and muscle tissues of common carp, cyprinus carpio, and nile tilapia, oreochromis niloticus. | accumulation of cadmium in gill, liver, muscle and kidney tissues of cyprinus carpio and oreochromis niloticus were investigated in fish exposed to 0.5 ppm cadmium over 1, 15 and 30 days under controlled laboratory conditions. tissue accumulation of the metal was measured using atomic absorption spectrophotometric techniques. cadmium accumulation in gill, liver, kidney and muscle, tissues of c. carpio and o. niloticus exposed to metal for 1, 15 and 30 days increased significantly compared with t ... | 2014 | 24526282 |
| mercury concentrations in commercial fish species of lake phewa, nepal. | mercury (hg) concentrations in four commercial fish species (tilapia oreochromis niloticus, spiny eel mastacembelus armatus, african catfish clarias gariepinus, and sahar tor putitora), were investigated in lake phewa, nepal. mean values of total mercury (thg mg kg(-1), ww) in these fishes were 0.02, 0.07, 0.05, and 0.12 respectively. methylmercury contributed 82 % of thg. the lowest value was detected in o. niloticus, an exclusive plant feeder. the biomagnification rate of hg through the fish c ... | 2013 | 23839154 |
| transcriptome comparison reveals a genetic network regulating the lower temperature limit in fish. | transcriptional plasticity is a major driver of phenotypic differences between species. the lower temperature limit (ltl), namely the lower end of survival temperature, is an important trait delimiting the geographical distribution of a species, however, the genetic mechanisms are poorly understood. we investigated the inter-species transcriptional diversification in cold responses between zebrafish danio rerio and tilapia oreochromis niloticus, which were reared at a common temperature (28 °c) ... | 2016 | 27356472 |
| cloning, molecular characterization, and expression analysis of the unc45 myosin chaperone b(unc45b)gene of grass carp (ctenopharyngodon idellus). | unc45 myosin chaperone b(unc45b)gene is a molecular chaperone that mediates the folding, assembly and accumulation of thick-filament myosin in the formation of sarcomere, which plays an important role in the development of striated muscle and the stability of sarcomere. in this study, the complete cdna sequence of unc45b gene of grass carp was obtained by rapid amplification of cdna ends (race), and the characteristics of the unc45b protein predicted from gene sequence was analyzed by bioinforma ... | 2016 | 27334505 |
| molecular characterization and expression analyses of cdnas encoding the thioredoxin-interacting protein and selenoprotein p genes and histological changes in nile tilapia (oreochromis niloticus) in response to silver nanoparticle exposure. | herein, nile tilapia thioredoxin-interacting protein (on-txnip) and selenoprotein p (on-sepp) cdnas were cloned and characterized. the full-length on-txnip cdna contained 2 arrestin domains, 2 conserved cysteine residues that bind to thioredoxin to inhibit thioredoxin function, and 2 ppxy motifs, which negatively regulate the protein by stimulating binding to e3 ubiquitin ligase. the on-sepp cdna contained 17 selenocysteines (sec) encoded by the tga codon, which can be recognized as either a sto ... | 2016 | 26621381 |
| systematic variation in the pattern of gene paralog retention between the teleost superorders ostariophysi and acanthopterygii. | teleost fish underwent whole-genome duplication around 450 ma followed by diploidization and loss of 80-85% of the duplicated genes. to identify a deep signature of this teleost-specific whole-genome duplication (tsgd), we searched for duplicated genes that were systematically and uniquely retained in one or other of the superorders ostariophysi and acanthopterygii. tsgd paralogs comprised 17-21% of total gene content. some 2.6% (510) of tsgd paralogs were present as pairs in the ostariophysi ge ... | 2014 | 24732281 |
| revealing the mechanisms of the rostral shift of pelvic fins among teleost fishes. | in teleost fishes, the position of the pelvic fins shift during evolution; this positional shift seems to have diversified their locomotion and feeding behavior, thereby expanding the habitats of these fishes. thus, such a positional shift of the pelvic fins is one of the significant features of teleost fishes from evolutionary, embryological, and taxonomic viewpoints, but no studies to date have investigated the mechanism for the rostral shift of the pelvic fins from the anal region in teleosts ... | 2011 | 21740511 |
| in search of determinants: gene expression during gonadal sex differentiation. | the diversity of inputs that guide sexual fate during development is both intriguing and daunting. in the field of fish biology, the study of sex determination is of great importance. for example, in aquaculture, sexually dimorphic growth rates and overall size leads to one sex being more marketable than the other. moreover, for breeding purposes it is important to maintain balanced sex ratios. furthermore, sex determination is sensitive to environmental factors, such as temperature and contamin ... | 2010 | 20557645 |
| japanese medaka hox paralog group 2: insights into the evolution of hox pg2 gene composition and expression in the osteichthyes. | hox paralog group 2 (pg2) genes function to specify the development of the hindbrain and pharyngeal arch-derived structures in the osteichthyes. in this article, we describe the cdna cloning and embryonic expression analysis of japanese medaka (oryzias latipes) hox pg2 genes. we show that there are only two functional canonical hox genes, hoxa2a and b2a, and that a previously identified hoxa2b gene is a transcribed pseudogene, psihoxa2b. the functional genes, hoxa2a and b2a, were expressed in de ... | 2008 | 18850588 |
| comparative analysis of hox paralog group 2 gene expression during nile tilapia (oreochromis niloticus) embryonic development. | the hindbrain and pharyngeal arch-derived structures of vertebrates are determined, at least in part, by hox paralog group 2 genes. in sarcopterygians, the hoxa2 gene alone appears to specify structures derived from the second pharyngeal arch (pa2), while in zebrafish (danio rerio), either of the two hox pg2 genes, hoxa2b or hoxb2a, can specify pa2-derived structures. we previously reported three hox pg2 genes in striped bass (morone saxatilis), including hoxa2a, hoxa2b, and hoxb2a and observed ... | 2007 | 17924140 |
| isolation and expression of tilapia (oreochromis niloticus) serine 8-type gnrh coding and regulatory sequences. | the complete serine 8-type gonadotropin releasing hormone (gnrh) coding sequence with a substantial 5-prime regulatory sequence (5 kb) has been isolated and characterised in nile tilapia (oreochromis niloticus) from a relevant genomic library. the primary structure of the protein precursor was identified for this gene. the promoter efficacy has been tested using 0.6 kb of the gnrh promoter driving a lacz reporter gene in both cultured spleen cells and transiently expressing zebrafish. in the cel ... | 2003 | 12568719 |
| nonlinkage of major histocompatibility complex class i and class ii loci in bony fishes. | in tetrapods, the functional (classical) class i and class ii b loci of the major histocompatibility complex (mhc) are tightly linked in a single chromosomal region. in an earlier study, we demonstrated that in the zebrafish, danio rerio, order cypriniformes, the two classes are present on different chromosomes. here, we show that the situation is similar in the stickleback, gasterosteus aculeatus, order gasterosteiformes, the common guppy, poecilia reticulata, order cyprinodontiformes, and the ... | 2000 | 10663573 |
| isolation of complementary dnas coding for a receptor for activated c kinase (rack) from zebrafish (danio rerio) and tilapia (oreochromis niloticus): constitutive developmental and tissue expression. | : we have cloned and sequenced complementary dnas coding for a receptor for activated c kinase (rack) from two species of teleost fishes, zebrafish (danio rerio) and tilapia (oreochromis niloticus). the tilapia clone is 1063 nucleotides long, and the zebrafish clone is 1069 nucleotides long. both clones contain an open reading frame coding for the complete rack protein of 317 amino acids. northern hybridization analysis using these clones as probes detected a 1.2-kb band, indicating that these a ... | 1999 | 10384002 |
| introduction of modern endocrine techniques for the production of monosex population of fishes. | of the techniques available to mass-produce a sterile or monosex population, the hormonal induction of sex reversal is the most widely practiced. this paper presents the synthesis of works done on sex reversal using 17α methyltestosterone (17αmt), 17β estradiol, non-steroidal aromatase inhibitors (ais) and oestrogen receptor agonist, tamoxifen in commercially important aquaculture species mossambique tilapia oreochromis mossambicus, nile tilapia oreochromis niloticus, common carp cyprinus carpio ... | 2013 | 23063432 |
| heavy metal (lead, cadmium) and antibiotic (tetracycline and chloramphenicol) residues in fresh and frozen fish types (clarias gariepinus, oreochromis niloticus) in ibadan, oyo state, nigeria. | this study was carried out to assess the level of heavy metals (lead (pb) and cadmium (cd)) and antibiotics (tetracycline and chloramphenicol) residues in frozen and fresh fish types obtained from eleyele river, officer's mess, alfa farm and a major frozen meat outlet in ibadan, oyo state nigeria. the atomic absorption spectrophotometer (aas) and high performance liquid chromatography (hplc) were used to analyze the heavy metals and antibiotics residue levels in fresh and frozen fish, respective ... | 2012 | 24205760 |
| assessment of heavy metals in water samples and tissues of edible fish species from awassa and koka rift valley lakes, ethiopia. | the ethiopian rift valley lakes host populations of edible fish species including oreochromis niloticus, labeobarbus intermedius and clarias gariepinus, which are harvested also in other tropical countries. we investigated the occurrence of six heavy metals in tissues of these fish species as well as in the waters of lake koka and lake awassa. both lakes are affected by industrial effluents in their catchments, making them ideal study sites. mercury concentrations were very low in the water samp ... | 2013 | 22821322 |
| in situ evaluation of the genotoxic potential of the river nile: ii. detection of dna strand-breakage and apoptosis in oreochromis niloticus niloticus (linnaeus, 1758) and clarias gariepinus (burchell, 1822). | this work is part of a wider eco-toxicological study proposed to evaluate the biological impact of contaminants along the whole course of the river nile, egypt. here we present data on the presence of dna strand-breaks and apoptotic cells assessed by use of comet and diffusion assays in erythrocytes of nile tilapia (oreochromis niloticus niloticus) and african catfish (clarias gariepinus). the results showed high degrees of dna damage and increased frequencies of apoptotic nuclei in blood of fis ... | 2012 | 22525358 |
| bioaccumulation of persistent organic pollutants (pops) in fish species from lake koka, ethiopia: the influence of lipid content and trophic position. | the concentrations and bioaccumulation of persistent organic pollutants (pops) were determined in four fish species from lake koka, ethiopia, representing 2-3 levels in the food chain of the lake. dichlorodiphenyltrichloroethanes (ddts), endosulfans, polychlorinated biphenyls (pcbs) and chlorpyrifos were identified, with ddts as the most predominant pesticide, with concentration ranging from 0.05 to 72.53ngg(-1) wet weight (ww). all fish tissue samples collected from different species of the lak ... | 2011 | 21978619 |
| isolation and characterisation of tilapia beta-actin promoter and comparison of its activity with carp beta-actin promoter. | the regulatory sequence including proximal promoter, untranslated exon 1 and intron 1 of the beta-actin gene from tilapia (oreochromis niloticus) has been isolated and spliced to a beta-galactosidase reporter gene to test its activity. comparisons of promoter activity have been carried out with three different constructs: (1) 1.6 kb tilapia beta-actin regulatory sequence, (2) 1.5 kb carp beta-actin regulatory sequence, and (3) 4.7 kb carp beta-actin regulatory sequence. although the 1.6 kb tilap ... | 2003 | 12527420 |
| fiber connections of the nucleus isthmi in the carp (cyprinus carpio) and tilapia (oreochromis niloticus). | the nucleus pretectalis (np) is a prominent nucleus in the percomorph pretectum and has been shown to project to the nucleus isthmi in the filefish by an hrp tract-tracing method [ito et al., 1981], but a homologous nucleus to the np is apparently lacking in ostariophysans. the present study examined fiber connections of the nucleus isthmi in an ostariophysan teleost, the carp (cyprinidae, cyprinus carpio), to identify a nucleus homologous to the percomorph nucleus pretectalis. identical studies ... | 2001 | 11964496 |
| effects of cypermethrin on antioxidant enzyme activities and lipid peroxidation in liver and kidney of the freshwater fish, oreochromis niloticus and cyprinus carpio (l.). | | 2001 | 11911634 |
| construction and characterization of bac libraries for three fish species; rainbow trout, carp and tilapia. | bacterial artificial chromosome (bac) libraries are important tools for genomic research. we have constructed seven genomic bac libraries from three fish species, rainbow trout (oncorhynchus mykiss), carp (cyprinus carpio) and tilapia (oreochromis niloticus). the two rainbow trout bac libraries have average insert sizes of 58 and 110 kb. the average size of inserts in the carp bac library is 160 kb. the average insert sizes of the four tilapia bac libraries are 65, 105, 145 and 194 kb, respectiv ... | 2001 | 11531698 |
| micronuclei monitoring of fishes from lake paranoá, under influence of sewage treatment plant discharges. | fish micronuclei tests (mn) were used to evaluate the ability of wastewater from two municipal sewage treatment plants that empty into lake paranoá to cause genetic damage. there were no significant differences in the frequencies of micronuclei between the control and hypertrophic areas. in contrast, cyclophosphamide and mitomycin c, used to test the sensitivity of the biological assay, significantly increased the micronuclei counts in tilapia rendalli, oreochromis niloticus and cyprinus carpio, ... | 2001 | 11287296 |
| copy number related transgene expression and mosaic somatic expression in hemizygous and homozygous transgenic tilapia (oreochromis niloticus). | three lines of transgenic tilapia (oreochromis niloticus) fish were generated with a construct containing a lacz reporter gene spliced to a 4.7 kb 5' regulatory region of a carp beta actin gene. all these three lines contain different copy numbers of transgenes and the levels of lacz expression were found to be related to transgene copy number. mosaic patterns of somatic lacz expression were observed in these three lines which differed between lines but were consistent within a line. we also obs ... | 2000 | 11206970 |
| differential in vitro suppressive effects of steroids on leukocyte phagocytosis in two teleosts, tilapia and common carp. | the objectives of this study were to investigate the potential roles of cortisol and gonadal steroids in the phagocytic activity of peripheral blood leukocytes in two teleosts, tilapia (oreochromis niloticus x o. aureus) and common carp (cyprinus carpio). an in vitro microtiter plate assay, measuring incorporation of fitc-latex beads into peripheral blood leukocytes, was developed for the first time in teleosts. peripheral blood leukocytes were cultured in al medium with tested compounds in a mi ... | 2001 | 11178882 |
| metabolic fractionation of stable carbon isotopes: implications of different proximate compositions for studies of the aquatic food webs using δ(13)c data. | in two laboratory experiments using tilapia (oreochromis niloticus) and common carp (cyprinus carpio), we investigated the effect of lipid content in the fish carcass on the stable carbon isotope ratio (δ(13)c). in both experiments, a significant negative correlation was found between lipid content in the carcass dry matter and δ(13)c of total dry matter, but there was no influence on the δ(13)c of fat-free dry matter or lipids. as the lipid content of fish is known to vary with reproductive sta ... | 1998 | 28308424 |
| morphometry of the kidneys of fresh water percoid and ostariophysian teleosts. | kidneys of four fresh water teleost species representing percormorpha (oreochromis niloticus and micropterus salmoides) and ostariophysi (cyprinus carpio and clarias mossambicus) were studied for quantitative structural characteristics using tissues fixed by perfusion. the renosomatic index (weight of kidney/weight of fish) was higher in the ostariophysian than percoid fish (p < 0.05) but values of volume of nephronic tissue/body weight were not significantly different. results obtained by point ... | 1997 | 9199699 |
| the effects of gonadal development and sex steroids on growth hormone secretion in the male tilapia hybrid (oreochromis niloticus × o. aureus). | profiles of plasma growth hormone (gh) in male tilapia hybrid (oreochromis niloticus x o. aureus) were measured and compared at different times of the year. the profiles did not appear to be repetitive, however, differences in their nature were observed at the different seasons; the most erratic profiles were seen in the height of the reproductive season (july), while the peaks were more subdued in the spring and disappeared in the autumn. peaks in male fish were more prominent than in the femal ... | 1995 | 24197495 |
| structure and sequence of the growth hormone-encoding gene from tilapia nilotica. | we report here the nucleotide (nt) sequence of the growth hormone (gh)-encoding gene (gh) of the tilapia fish (tilapia nilotica). the t. nilotica gh gene, similar to that of the salmonidae fish, atlantic salmon and rainbow trout, contains six exons and five introns. however, despite the presence of an additional intron (intron v), the size of the primary transcript of t. nilotica gh (1666 nt) is significantly shorter than that of all other currently characterized fish gh genes. comparison of seq ... | 1992 | 1572545 |
| electrocardiograms in five bipolar leads recorded from the body surface of three fish species (cyprinus carpio, oreochromis niloticus and pagrus major) in fresh or sea-water. | 1. ecgs were recorded using five bipolar leads from the body surface of porgy pagrus major and tilapia oreochromis niloticus in fresh or sea-water, or held on a dry towel. 2. the maximum qrs amplitude was detected in lead v in both species being 54 +/- 25 microv in porgy and 241 +/- 78 microv in tilapia, respectively, when the fish were held on the dry towel. 3. clear ecg waves could not be obtained from porgy in sea-water because of the porgy's small cardiac potential and leakage of the potenti ... | 1988 | 2907442 |
| production of oocytes of nile tilapia (oreochromis niloticus) for in vitro fertilization via hormonal treatments. | only a few studies have described hormonal treatments for induction of synchronicity and gamete collection in nile tilapia (oreochromis niloticus), both important for assortative matings in breeding programmes and essential for polyploidy technologies. in this study, we compared the effectiveness of carp pituitary extract (cpe), nile tilapia pituitary extract (tpe), human chorionic gonadotropin (hcg) and gonadotropin-releasing hormone (gnrh) protocols on the induction of spawning and egg product ... | 2013 | 23834584 |
| gene transfer and mutagenesis mediated by sleeping beauty transposon in nile tilapia (oreochromis niloticus). | the success of gene transfer has been demonstrated in many of vertebrate species, whereas the efficiency of producing transgenic animals remains pretty low due to the random integration of foreign genes into a recipient genome. the sleeping beauty (sb) transposon is able to improve the efficiency of gene transfer in zebrafish and mouse, but its activity in tilapia (oreochromis niloticus) has yet to be characterized. herein, we demonstrate the potential of using the sb transposon system as an eff ... | 2013 | 23417791 |
| cytochrome p450 1c1 complementary dna cloning, sequence analysis and constitutive expression induced by benzo-a-pyrene in nile tilapia (oreochromis niloticus). | cyp1c is the newest member of the cyp1 family of p450s; however, its physiological significance, inducers, and metabolic functions are unknown. in this study, a new complementary dna of the cyp1c subfamily encoding cyp1c1 was isolated from nile tilapia (oreochromis niloticus) liver after intracoelomic injection with benzo-a-pyrene (bap). the full-length cdna was 2223 base pair (bp) long and contained an open reading frame of 1581 bp encoding a protein of 526 amino acids and a stop codon. the seq ... | 2012 | 22204985 |
| assessment of the genotoxic impact of pesticides on farming communities in the countryside of santa catarina state, brazil. | the aim of this study was to assess the use of pesticides on farms located in the lambedor river watershed in guatambu, state of santa catarina, as well as to determine, by micronucleus testing, the risk of genotoxic impact. samples from locally collected cyprinus carpio, hypostomus punctatus, rhamdia quelen and oreochromis niloticus gave evidence of a mean increase in micronuclei frequency from 6.21 to 13.78 in 1,000 erythrocytes, a clear indication of the genotoxic potenciality of pesticide re ... | 2011 | 21637554 |
| tailfin clipping, a painful procedure: studies on nile tilapia and common carp. | the fish welfare debate is intensifying. consequently, more research is carried out to further our knowledge on fish welfare in aquaculture. we define here a series of key parameters to substantiate an acute response to a supposedly painful stimulus: a standardized tailfin clip. ultrastructural analysis of common carp (cyprinus carpio) tailfin indicates the presence of a-δ and c-type axons, which are typical for transmitting nociceptive signals in (higher) vertebrates. in nile tilapia (oreochrom ... | 2010 | 20705079 |
| profile of micronucleus frequencies and dna damage in different species of fish in a eutrophic tropical lake. | lake paranoá is a tropical reservoir for the city of brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. at present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. we evaluated both genotoxic and cytotoxic parameters in several fish species (geophagus brasiliensis, cichla temensis, hoplias malabaricus, astyanax bimaculatus lacustres, oreochromis ... | 2009 | 21637659 |