| steroid sulphatase: expression, isolation and inhibition for active-site identification studies. | steroid sulphatase, which can hydrolyse 3-hydroxysteroid sulphates, has important roles in several physiological and pathological processes. a number of steroid sulphatase inhibitors have now been developed, of which the most potent to date is oestrone-3-o-sulphamate (emate). this inhibitor inactivates steroid sulphatase in an irreversible, time- and concentration-dependent manner. in order to be able to use a radiolabelled derivative of emate to study the active site, it will be essential to pr ... | 1998 | 9566745 |
| core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant n-linked oligosaccharides and is present in a wide variety of plant extracts. | carbohydrates have been suggested to account for some ige cross-reactions between various plant, insect, and mollusk extracts, while some igg antibodies have been successfully raised against plant glycoproteins. a rat monoclonal antibody raised against elderberry abscission tissue (yz1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-linked immunosorbent assay against a range of plant glycoproteins and extracts as well as neoglycoprotein ... | 1998 | 9621106 |
| scavestrogen sulfamates: correlation between estrone sulfatase inhibiting and antioxidant effects. | in the present study estrone sulfatase (steryl-sulfatase; ec 3.1.6.2) and phenylsulfatase (arylsulfatase b; ec 3.1.6.1) inhibiting as well as antioxidant effects exerted by ring b,c unsaturated sulfamates of estrone (j 1025), 17 beta-estradiol (j 1054, j 1059, j 1067), and 17 alpha-estradiol (j 1051, j 1064, j 1065) were examined as compared with their parent compounds, j 994, j 995, and j 1050, using six different in vitro models: (i) estrone sulfatase activity in human placental microsomes, (i ... | 1998 | 9635147 |
| screening and confirmatory determination of ractopamine residues in calves treated with growth promoting doses of the beta-agonist. | ractopamine (rct) is a phenethanolamine member of the family of beta-adrenergic agonists (beta-agonists). this class of compounds have become notable for their properties of enhancing the growth rates of farm animal species but are not licensed for use in europe. an elisa procedure employing a polyclonal antibody raised in a goat was developed to detect rct residues in bovine urine samples. the assay had a high sensitivity (calibration curve mid-point of 22 pg per well), allowing the analysis of ... | 1998 | 9709494 |
| assembly and architecture of invertebrate cytoplasmic intermediate filaments reconcile features of vertebrate cytoplasmic and nuclear lamin-type intermediate filaments. | the two major intermediate filament (if) proteins from the esophagus epithelium of the snail helix pomatia and the two major if proteins from muscle tissue of the nematode ascaris suum were investigated under a variety of assembly conditions. the lowest-order complexes from each of the four protostomic invertebrate (p-inv) if proteins are parallel, unstaggered dimers involving two-stranded alpha-helical coiled coil formation of their approximately 350 amino acid residue central rod domain (i.e. ... | 1998 | 9737925 |
| protostrongylid nematode infection of chamois (rupicapra rupicapra) at the bauges massif (french alps). | from 1979 to 1985, protostrongylid infections (small lungworms) of chamois were studied at the bauges hunting reserve, in the western alps. fifty-two chamois were necropsied on 24 sites and lung nematodes identified to species. from june to november 1985, a more detailed study was undertaken on four sites where chamois were the only ruminants. first-stage larvae excreted in feces and infection of snails intermediate hosts were monitored. the protostrongylid species were, arranged in decreasing o ... | 1998 | 9746285 |
| regulation of ocular mucin secretion by p2y2 nucleotide receptors in rabbit and human conjunctiva. | the effects of adenine analogues on secretion of high molecular weight, mucin-like glycoproteins (mucins) by conjunctival goblet cells were investigated using isolated rabbit and human conjunctiva. mucin secretion was assayed using a quantitative dot-blot assay of helix pomatia agglutinin-horseradish peroxidase binding to mucins absorbed to nitrocellulose filters. in rabbit conjunctiva, exogenous atp (10(-7)-10(-3) m) induced a concentration-dependent, four-fold increase in mucin secretion that ... | 1998 | 9778415 |
| detection of inflammation- and neoplasia-associated alterations in human large intestine using plant/invertebrate lectins, galectin-1 and neoglycoproteins. | commonly, plant and invertebrate lectins are accepted glycohistochemical tools for the analysis of normal and altered structures of glycans in histology and pathology. mammalian lectins and neoglycoproteins are recent additions to this panel for the detection of lectin-reactive carbohydrate epitopes and glycoligand-binding sites. the binding profiles of these three types of probes were comparatively analyzed in normal, inflamed and neoplastic large intestine. in normal colonic mucosa the intrace ... | 1998 | 9780361 |
| viral glycoproteins accumulate in newly formed annulate lamellae following infection of lymphoid cells by human herpesvirus 6. | ultrastructural analysis of hsb-2 t-lymphoid cells and human cord blood mononuclear cells infected with human herpesvirus 6 revealed the presence, in the cell cytoplasm, of annulate lamellae (al), which were absent in uninfected cells. time course analysis of the appearance of al following viral infection showed that no al were visible within the first 72 h postinfection and that their formation correlated with the expression of the late viral glycoprotein gp116. the requirement of active viral ... | 1998 | 9811708 |
| lectin histochemistry of dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats. | a lectin histochemical study was carried out on the dorsal skin of wistar-derived hypotrichotic wbn/ila-ht rats (htrs) and wistar rats (wrs) at 3, 7 and 24 weeks of age to clarify the lectinhistochemical characteristics of the skin during their development. the lectins examined were concanavalia ensiformis (con a), dolichos biflorus agglutinin (dba), griffonia simpliciolia (gs-i), helix pomatia agglutinin (hpa), arachis hypogaea (pna), glycine maximus agglutinin (sba), ulex europeus agglutinin ( ... | 1998 | 9816494 |
| novel forms of protein glycosylation. | a large number of new glycans, derived from glycoproteins, has been characterized in the past few years. o-linked fucose was found in epidermal growth factor-like domains of several proteins. for the n-linked glycans of helix pomatia hemocyanin, novel types of antennae were identified. the positions of noncarbohydrate substituents were established in n-glycans. c-mannosylation of a tryptophan residue was discovered in human ribonuclease 2 and is the first example of c-glycosylation in glycoprote ... | 1998 | 9818259 |
| altered anionic gbm components in monoclonal antibody against slit diaphragm-injected proteinuric rats. | we previously reported that monoclonal antibody (mab) 5-1-6 bound to renal filtration slits induces massive proteinuria without causing ultrastructural changes in the glomerulus. this study evaluated the underlying mechanisms of the increase in glomerular permeability. | 1998 | 9844125 |
| comparison of four immunoassays for the detection of lorazepam in urine. | four healthy patient subjects were each given a single, 1-mg lorazepam tablet. urine samples from all patient subjects were collected at 12 intervals (0-2, 2-5, 5-8, 8-11, 11-14, 14-24, 24-26, 26-29, 29-32, 32-35, 35-38, and 38-48 hours). an aliquot from each urine collection was screened using cloned enzyme donor immunoassay (cedia), enzyme-multiplied immunoassay technique (emit) ii, emit dau, and fluorescence polarization immunoassay (fpia) without and with hydrolysis using beta-glucuronidase. ... | 1998 | 9853986 |
| muc5ac mucin is a component of the human precorneal tear film. | mucins are important structural and functional components of the precorneal tear film, yet little is known of their composition and synthesis. the mrnas of muc1, muc4, and muc5ac have previously been identified in human conjunctiva. of these, only muc5ac mrna appears to be associated with goblet cells. the purpose of the this study was to quantify muc5ac transcript levels, to identify muc5ac protein in conjunctiva, tears, and goblet cells and to determine whether this mucin is secreted in respon ... | 1999 | 9888425 |
| innate immunity in insects: the role of multiple, endogenous serum lectins in the recognition of foreign invaders in the cockroach, blaberus discoidalis. | unlike vertebrates, insects do not have an ab-based nonself recognition system, and must rely totally on innate immunity to defend themselves from microbial invaders. the most likely candidates for recognizing foreign material in insects are the lectins, which have already been shown to be important in mammalian innate immunity. the hemolymph of the cockroach, blaberus discoidalis, contains multiple lectins, designated bdl1, bdl2, bdl3, and gsl (beta-1,3-glucan-specific lectin), two of which, na ... | 1999 | 9973417 |
| the use of the lectin helix pomatia agglutinin (hpa) as a prognostic indicator and as a tool in cancer research. | progress in treatment for cancer has enabled extension of the disease-free interval, and of the quality of life for patients, but there has been very little improvement in overall survival rates. the main reason for this has been the ineffectiveness of current therapies to kill all the cancer cells once they have spread to distant sites to form metastatic deposits. one marker which has proved to be useful in identifying those cancers which have the potential to spread is the lectin helix pomatia ... | 1999 | 9987666 |
| a two-component affinity chromatography purification of helix pomatia arylsulfatase by tyrosine vanadate. | the inhibition of helix pomatia arylsulfatase by the synergistic combination of n-acetyl-l-tyrosine ethyl ester and vanadate has been extended to affinity chromatography for purification. in the presence of vanadate, l-tyrosine ethyl ester (tee), immobilized on ch-sepharose 4b retained arylsulfatase from the digestive juice or lyophilized powder of h. pomatia. no enzyme was retained without vanadate or with arsenate or phosphate. arylsulfatase was eluted from the column matrix by removing the va ... | 1999 | 10049673 |
| glycoconjugate expression in follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in specific pathogen-free and conventional rats. | we examined lectin-histochemically the glycoconjugate expression in the follicle-associated epithelium (fae) covering the nasal-associated lymphoid tissue (nalt) in the rat under specific pathogen-free (spf) and conventional (cv) conditions and compared the results for spf and cv rats as well as for membranous (m) cells and adjacent ciliated respiratory epithelial (cre) cells in fae. n-acetylgalactosamine-specific lectins, dolichos biflorus (dba), helix pomatia (hpa), glycine max (sba) and vicia ... | 1999 | 10067202 |
| [the bronchorelaxant effect of helicidine, a helix pomatia extract, interferes with prostaglandin e2]. | helicidine is a biological extract prepared from the snail helix pomatia l. and used in man as an anti-tussive agent. however, its mechanisms of action are not fully defined. in this study, we have investigated a possible relaxant effect of helicidine on guinea-pig airway smooth muscle and evaluated the role of prostanoids and airway epithelium in this relaxation. h. pomatia extract (0.001-1 mg/ml) induced a dose-dependent relaxation of guinea-pig trachea pre-contracted with histamine both in th ... | 1999 | 10081783 |
| s100 calcium binding protein affects neuronal electrical discharge activity by modulation of potassium currents. | s100 calcium binding protein has been associated with a variety of intra- and extracellular calcium-mediated functions, including learning and memory. we have previously localized s100-immunoreactive neurons correlated with spontaneous discharge activity in the central nervous system of the mollusc, helix pomatia. in this study, we further investigated the effects of s100 (s100b and s100a1) on electrical discharge activity and membrane currents of helix neurons, using current- and voltage-clamp ... | 1999 | 10215154 |
| [use of estrone sulfate determination in goat blood for the detection of pregnancy and prediction of fetal number]. | the study was carried out on 50 goats of polish white breed. blood samples were taken on day 60 after mating from v. jugularis. pregnancy diagnosis was made using transcutan ultrasound scanning (pie medical scanner 2000 with a 5 mhz curved array probe). the estrone sulphate level was determined by ria method after extraction and hydrolysis with arylsulphatase/glucuronidase from helix pomatia. as a threshold value for differentiation of pregnant from nonpregnant goats 1 ng/ml was assumed. in eigh ... | 1999 | 10326236 |
| intracellular transport and maturation pathway of human herpesvirus 6. | a peculiar characteristic of cells infected with human herpesvirus 6 (hhv6) is the absence of viral glycoproteins on the plasma membrane, which may reflect an atypical intracellular transport of the virions and/or the viral glycoproteins, different from that of the other members of the herpesvirus family. to investigate the maturation pathway of hhv-6 in the human t lymphoid cell line hsb-2, we used lectin cytochemistry and immunogold labeling combined with several electron microscopical techniq ... | 1999 | 10329556 |
| lectin binding sites in parotid acinar secretory granules of normal and isoproterenol treated rat. | lectin staining patterns in secretory granules of rat parotid gland acinar cell of untreated and isoproterenol-injected animals were examined by electron microscopy. we used four lectin-gold complexes: ulex europaeus agglutinin i (uea-i), helix pomatia agglutinin (hpa), wheat germ agglutinin (wga), glycine max agglutinin (sba). specimens were low temperature embedded in the hydrophilic lowicryl k4m resin. the normal acinar cells produced glycoconjugates which were positive for all of the lectins ... | 1999 | 10363359 |
| beta2-agonist abuse in food producing animals: use of in vitro liver preparations to assess biotransformation and potential target residues for surveillance. | 1. the biotransformation of [3h]clenbuterol, [3h]salbutamol, [14c]salmeterol and 7-ethoxycoumarin by bovine liver was investigated by incubation with freshly prepared microsomes, suspension and monolayer cultures of isolated hepatocytes, precision-cut (250 microm) and chopped (600 microm) tissue slices. 2. radio-hplc analysis indicated that the saligenin beta2-agonists salmeterol and salbutamol were extensively metabolized by all intact cell preparations. a single major product (smm1) was eviden ... | 1999 | 10379986 |
| enzyme immunoassay for the measurement of 17alpha-estradiol 17-n-acetylglucosaminide in rabbit urine. | 17alpha-estradiol 17-n-acetylglucosaminide (17alphae2 17nag) is an estrogen metabolite hitherto obtained only in rabbits. to gain insight into this unique conjugate, an enzyme immunoassay (eia) was established by using antiserum elicited against 3-[3-(1-carboxypropyl)] ether of 17alphae2 17nag-bovine serum albumin conjugate; horseradish peroxidase, as a label; and 3,3',5,5'-tetramethylbenzidine, as a chromogen. the method proved to be specific, and the detection range of the assay was 0.20-10.00 ... | 1999 | 10399888 |
| determination of oestrogen concentrations in bovine plasma by a recombinant oestrogen receptor-reporter gene yeast bioassay. | a recombinant cell yeast bioassay (rcba) was applied to the generic measurement of bovine plasma oestrogen concentration. samples were prepared by diethyl ether extraction of plasma following addition of [3h]17 beta-oestradiol as internal standard; organic and aqueous phases were separated by freezing (recovery 97.1 +/- 0.7%) and dried extract reconstituted in culture medium (recovery 31.4 +/- 4.5%). plasma oestrogen concentrations were measured by incubation of extracts with yeast containing a ... | 1998 | 10435304 |
| utility of isolated hepatocytes and radio-hplc-msn for the analysis of the metabolic fate of 19-nortestosterone laurate in cattle. | the metabolic fate of 19-nortestosterone laurate in cattle was investigated to evaluate target analyte(s) appropriate to surveillance for illicit use as a growth promoting agent. bovine hepatocytes were incubated with either [3h]19-nortestosterone laurate (19-ntl; 4-estren-17 beta-laurate-3-one) or [3h]19-nortestosterone (19-nt; 4-estren-17 beta-ol-3-one; nandrolone). hepatocyte medium was extracted with solid phase c18 media and analysed by narrow bore radio-hplc-msn (lcq, finnigan) to evaluate ... | 1998 | 10435305 |
| interference of helix pomatia extracts on the determination of methandriol in veterinary residue control analysis. | aqueous solutions containing methandriol (mad) were incubated with succus helix pomatia (shp) or beta-glucuronidase from escherichia coli (ec). shp, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of mad into methyltestosterone. no conversion occurred when bacterial beta-glucuronidase from e. coli was used. | 1998 | 10435316 |
| abalone (haliotis tuberculata) hemocyanin type 1 (hth1). organization of the approximately 400 kda subunit, and amino acid sequence of its functional units f, g and h. | we have identified two separate hemocyanin types (hth1 and hth2) in the european abalone haliotis tuberculata. hth1/hth2 hybrid molecules were not found. by selective dissociation of hth2 we isolated hth1 which, as revealed by electron microscopy and sds/page, is present as didecamers of a approximately 400 kda subunit. immunologically, hth1 and hth2 correspond to keyhole limpet hemocyanin (klh)1 and klh2, respectively, the two well-studied hemocyanin types of the closely related marine gastropo ... | 1999 | 10447670 |
| [caragana arborescens lectin--a marker of myoepitheliocytes of salivary glands]. | submaxillary salivary gland preparations of a horse, a cow, a pig, a rabbit and white outbred rats were stained with peroxidase-labeled lectins of different carbohydrate specificity. it was found that n-acetyl-d-galactosamine-specific lectins (caragana arborescens lam., phaseolus unatus seeds, helix pomatia sails) bind selectively with myoepitheliocytes. best marker of the latter proved to be caragana arborescens lectin, consisting of two components which differ in their affinity to n-acetyl-d-g ... | 1999 | 10486811 |
| lectins in drug delivery: a study of the acute local irritancy of the lectins from solanum tuberosum and helix pomatia. | lectins are proteins or glycoproteins of non-immune origin capable of binding to one or more specific sugar residues. the potential for using lectins as a means of 'anchoring' a drug delivery system to the mucosal surfaces of the eye has been investigated in previous work, with the lectins from solanum tuberosum and helix pomatia showing particular promise. in this study the acute local dermal irritancy of these lectins, in terms of their potential to cause inflammation and tissue necrosis, was ... | 1999 | 10494002 |
| determination of urinary 18 beta-glycyrrhetinic acid by gas chromatography and its clinical application in man. | a sensitive and quantitative gas chromatographic assay for the determination of 18 beta-glycyrrhetinic acid (18 beta-ga), the main metabolite of glycyrrhizin after oral licorice consumption in human urine, has been developed and validated. for the extraction of 18 beta-ga from urine two sep-pak c18 extractions, hydrolysis with helix pomatia and three liquid-liquid extractions were performed, using 18 alpha-glycyrrhetinic acid (18 alpha-ga) as internal standard. both 18 beta-ga and internal stand ... | 1999 | 10510787 |
| development of a simple method for the determination of genistein, daidzein, biochanin a, and formononetin (biochanin b) in human urine. | a simple method was developed for the determination of free and/or total isoflavones daidzein, genistein, and their respective 4'-methoxy derivatives biochanin a and formononetin (biochanin b) at low levels in human urine. a solid-phase extraction on octadecyl silica (c(18)) columns was used for the isolation of the phytoestrogens from the matrix. an extraction on a chemelut 1010 column connected on-line to a florisil cartridge by a teflon stopcock was used for effective eluate purification. a m ... | 1999 | 10552674 |
| the rational design of semisynthetic peroxidases. | a semisynthetic peroxidase was designed by exploiting the structural similarity of the active sites of vanadium dependent haloperoxidases and acid phosphatases. incorporation of vanadate ion into the active site of phytase (e.c. 3.1.3.8), which mediates in vivo the hydrolysis of phosphate esters, leads to the formation of a semisynthetic peroxidase, which catalyzes the enantioselective oxidation of prochiral sulfides with h(2)o(2) affording the s-sulfoxide, e.g. in 66% ee at 100% conversion for ... | 2000 | 10581439 |
| the involvement of helix pomatia lectin (hpa) binding n-acetylgalactosamine glycans in cancer progression. | the lectin from helix pomatia, the roman snail (hpa), recognises terminal alpha n-acetylgalactosamine residues. a large number of lectin histochemical studies have demonstrated that expression of hpa-binding glycoproteins by cancer cells to be a marker of metastatic competence and poor prognosis in a range of common human adenocarcinomas, including those of breast, stomach, ovary, oesophagus, colorectum, thyroid and prostate. around 80% of metastases arising from primary breast cancer are predic ... | 2000 | 10668205 |
| effects of valproate derivatives i. antiepileptic efficacy of amides, structural analogs and esters. | derivatives of the antiepileptic drug valproate (vpa, 2-propylpentanoic acid) have been synthesized and tested in order to improve the intracellular availability of vpa. the buccal ganglia of helix pomatia were used as a test nervous system and antiepileptic efficacies were reconfirmed using rat cortex in vivo. epileptiform activities consisted of typical paroxysmal depolarization shifts (pds) which appeared in the identified neuron b3 with application of pentylenetetrazol. epileptiform activiti ... | 2000 | 10670421 |
| electrospray ionization mass spectrometry of zinc, cadmium, and copper metallothioneins: evidence for metal-binding cooperativity. | electrospray ionization (esi) mass spectra of both well-characterized and novel metallothioneins (mts) from various species were recorded to explore their metal-ion-binding modes and stoichiometries. the esi mass spectra of the zinc- and cadmium-binding mts showed a single main peak corresponding to metal-to-protein ratios of 4, 6, or 7. these findings combined with data obtained by other methods suggest that these mts bind zinc or cadmium in a single predominant form and are consistent with the ... | 2000 | 10716192 |
| lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood. | as only a few cell surface markers for dendritic cells (dc) have been identified to date, this study examined the expression of ligands for lectin on different human dc populations. the ability of concanavalin a (con a), wheat germ agglutinin (wga), peanut agglutinin (pna), and helix pomatia (hpa) to bind to cell lines and pbmc and dc populations was analyzed by flow cytometry and specificity of binding confirmed using inhibitory and noninhibitory sugars. the cell lines showed non-lineage-restri ... | 2000 | 10716881 |
| effects of lectins on calcification by vesicles isolated from aortas of cholesterol-fed rabbits. | advanced vascular calcification in atherosclerosis weakens arterial walls, thereby imposing a serious rupturing effect. however, the mechanism of dystrophic calcification remains unknown. although accumulating morphological and biochemical evidence reveals a role for calcifiable vesicles in plaque calcification, the mechanism of vesicle-mediated calcification has not been fully explored. to study whether vesicles' membrane components, such as carbohydrates, may have a role in vesicle-mediated ca ... | 2000 | 10727613 |
| histological disorders related to the focal disappearance of the epiphyseal growth plate in rats induced by high dose of vitamin a. | the histological disorders related to the focal disappearance of the epiphyseal growth plate were examined histochemically in the proximal tibia of rats administered a high dose of vitamin a. animals were given 100,000 iu/100 g body weight/day of vitamin a for 5 days from 4 weeks after birth (va rats) or given deionized water as control and sacrificed on day 12 and 19 of the experiment. tibiae were examined by immunohistochemistry for type i, ii and x collagens, lectin-histochemistry for helix p ... | 2000 | 10770602 |
| characterisation of lectin binding patterns of mouse bronchiolar and rat alveolar epithelial cells in culture. | lung epithelial cell differentiation pathways remain unclear. this is due in part to the plasticity of these cells and the lack of markers which accurately reflect their differentiation status. the aim of this study was to determine if lectin binding properties are useful determinants of functional differentiation status in vitro. mouse clara cells were cultured for 5 days. during this time, no alteration in differentiation was evident by electron microscopy. no significant alteration in binding ... | 2000 | 10805383 |
| stability and enzymatic hydrolysis of quaternary ammonium-linked glucuronide metabolites of drugs with an aliphatic tertiary amine-implications for analysis. | quaternary ammonium-linked glucuronide (n+-glucuronide) metabolites formed at aliphatic tertiary amine functional groups of xenobiotics have not been previously systematically studied with respect to their stability over a wide ph range and the ease of enzymatic hydrolysis by beta-glucuronidase from various sources. three and four n+-glucuronide metabolites were respectively studied regarding their non-enzymatic and enzymatic stabilities where the metabolites were quantified by hplc procedures. ... | 2000 | 10815723 |
| development of conjunctival goblet cells and their neuroreceptor subtype expression. | to investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells. | 2000 | 10892854 |
| time-dependent actions of aluminates on membrane and action potentials of snail neurons. | aluminum (al) is one of the elements, which is frequently subjected to experiments, however, the neurological observations with it are rather conflicting. the cause of this controversiality is not known but relates to some human disorders such as alzheimer's disease and others as well. we studied the time-dependent actions of alcl3 base solutions on resting membrane potential (em), input resistance (rin) and spike shape in giant neurons of the snail helix pomatia l. at ph 7.7 and room temperatur ... | 2000 | 11034155 |
| localized infusion of tunicamycin in rat hemimandibles: alteration of the basal lamina associated with maturation stage ameloblasts. | at the beginning of the maturation stage of amelogenesis, ameloblasts deposit a basal lamina (bl) at the interface between their apical surface and maturing enamel. this structure is rich in glycoconjugates and is proposed to exhibit adhesive and/or filtering functions. to clarify its role, we have applied a recently developed surgical window model to locally administer tunicamycin (tm), an antibiotic that interferes with n-glycosylation, in the rat hemimandible using an osmotic minipump. male w ... | 2001 | 11156685 |
| analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting. | alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce. | 2001 | 11170128 |
| enzymatic hydrolysis of conjugated steroid metabolites: search for optimum conditions using response surface methodology. | the optimum conditions for hydrolysing conjugated metabolites of steroid hormones in bovine urine were performed with helix pomatia juice, beta-glucuronidase from bovine liver and preparations of limpets and abalone entrails using response surface methodology. the experimental design and empirical modelling used allowed us to assess the main effects of factors (time, temperature, ph and enzyme quantity) and to predict the optimum conditions for each enzyme preparation. confirmatory experiments w ... | 2000 | 11219062 |
| helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. | predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. evidence suggests that the lectin helix pomatia agglutinin (hpa) identifies oligosaccharides associated with poor-prognosis cancer. our aim was to identify oligosaccharides that bind hpa in aggressive breast cancers. breast-cancer cell lines (mcf-7, bt-549 and bt-20) and a cell line from human milk (hbl-100), which showed a range ... | 2001 | 11241316 |
| n-acetylgalactosamine, n-acetylglucosamine and sialic acid expression in primary breast cancers. | binding of the lectin from helix pomatia (hpa), which recognises n-acetylgalactosamine and n-acetylglucosamine glycans, is a predictor of metastasis and poor prognosis in a number of human adenocarcinomas, including breast cancer. the glycoproteins to which it binds in these tumours have been only partially characterised, and the mechanisms underlying their biosynthesis remain unknown. in this study, 111 primary breast cancers were assessed for binding of hpa and labelling characteristics were c ... | 2001 | 11252626 |
| comparative lectin-histochemistry on the pre-epithelial mucus layer in the distal colon of conventional and germ-free rats. | the mucin composition of the rat distal colonic pre-epithelial mucus layer (pml) was studied by lectin histochemistry in conventional (cv), and germ-free (gf) rats to define effects exerted by the gut flora. no peanut agglutinin (pna) binding was observed in the pml of gf rats, while the pml of their cv counterparts showed a considerable pna linkage, indicating terminal gal-beta1,3-galnac residues. soybean agglutinin (sba) and helix pomatia agglutinin (hpa) stained the pml mucins in cv and in gf ... | 1998 | 11253810 |
| comparative study of blood group-recognizing lectins toward abo blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides. | binding specificities of abo blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. human serum albumin conjugated with a- and b-trisaccharides was clearly recognized by helix pomatia (hpa), phaseolus lunatus, dolichos biflorus agglutinins, and griffonia simplicifolia i agglutinin b(4), respectiv ... | 2001 | 11342253 |
| the detection of psilocin in human urine. | pharmacokinetic studies of psilocybin in humans have shown the rapid dephosphorylation of psilocybin to psilocin with further conversion to 4-hydroxy-tryptophole (4ht) and 4-hydroxyindole-3-acetic acid (4hiaa) in plasma. our study shows that psilocin also undergoes conjugation and can be found in the urine as the psilocin-glucuronide conjugate. recoveries after enzymatic hydrolysis of the urine with beta-glucuronidase (helix pomatia or e. coli) when compared to non-hydrolyzed urine confirmed the ... | 2001 | 11373000 |
| isolation, characterization, and propagation of rat conjunctival goblet cells in vitro. | to isolate, culture, and characterize goblet cells from the conjunctiva of rats. | 2001 | 11381047 |
| lectin binding patterns of uterine glands in mares with chronic endometrial degeneration. | to evaluate changes of glycoconjugate in uterine glands of endometrial tissues obtained from mares. | 2001 | 11400838 |
| galnac glycoprotein expression by breast cell lines, primary breast cancer and normal breast epithelial membrane. | over-expression of n-acetylgalactosamine glycoproteins as detected by binding of the lectin from helix pomatia (hpa), is associated with metastatic competence and poor patient prognosis in a range of human adenocarcinomas. these glycoproteins remain poorly characterised, and their functional role has yet to be elucidated. this study describes characterisation of a range of human breast/breast cancer cell lines for the expression of the n-acetylgalactosaminylated glycoproteins of interest, and th ... | 2001 | 11592774 |
| hydrolysis of conjugated metabolites of buprenorphine. i. the quantitative enzymatic hydrolysis of buprenorphine-3-beta-d-glucuronide in human urine. | buprenorphine, which is a powerful analgesic, a substitution drug for opioids widely used in europe, and a promising new drug currently undergoing clinical trials in the treatment of opioid dependence in the u.s., is excreted in human urine mainly as glucuronide conjugates. in gas chromatographic-mass spectrometric analysis, the urine specimens must be first hydrolyzed to release buprenorphine from its glucuronide conjugates. in order to evaluate the existing hydrolysis methods and to find the o ... | 2001 | 11599605 |
| proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer. | changes in glycosylation of glycoproteins and glycolipids is a common feature of cancer and may influence cancer cell behaviour, perhaps by enabling cell-cell interactions which favour metastasis or by allowing cancer cells to evade immuno-surveillance. studies to identify glycosylation changes in human cancer have often used immunohistochemical techniques with lectins or antibodies and human tissue sections. whilst some detailed biochemical studies have been performed there are few clinically r ... | 2001 | 11677781 |
| metal concentrations in helix pomatia, helix aspersa and arion rufus: a comparative study. | in this study we evaluated the differences between concentrations of copper, iron, zinc, manganese, lead and cadmium in three terrestrial gastropods: helix pomatia, helix aspersa and arion rufus, collected in a semi-rural location in northern italy. metal concentrations in the foot and in the digestive gland were measured. in the hepatopancreas, copper and zinc did not differ significantly in the three species; the levels of copper were also similar in the foot. in comparison to helix sps., a. r ... | 2001 | 11706793 |
| simultaneous determination of fifteen low-dosed benzodiazepines in human urine by solid-phase extraction and gas chromatography-mass spectrometry. | a gas chromatographic-mass spectrometric method was developed for the simultaneous analysis of 15 low-dosed benzodiazepines, both parent compounds and their corresponding metabolites, in human urine. the target compounds are alprazolam, alpha-hydroxyalprazolam, 4-hydroxyalprazolam, flunitrazepam, 7-aminoflunitrazepam, desmethylflunitrazepam, flurazepam, hydroxyethylflurazepam, nitrogen-desalkylflurazepam, ketazolam, oxazepam, lormetazepam, lorazepam, triazolam and alpha-hydroxytriazolam. nitroge ... | 2001 | 11767312 |
| cloning and sequencing of cdnas encoding for a novel copper-specific metallothionein and two cadmium-inducible metallothioneins from the blue crab callinectes sapidus. | metallothioneins (mts) are cysteine-rich metal-binding proteins found in micro-organisms, plants and all invertebrate and vertebrate animals. unicellular eukaryotes such as yeast have a copper-mt whose synthesis is induced by a copper-activated transcription factor. most higher organisms have two major cadmium/zinc mt isoforms, whose synthesis is controlled by a zinc-activated transcription factor. the blue crab, callinectes sapidus, has two cadmium-inducible isoforms, cdmt-i and cdmt-ii, and a ... | 2000 | 11790353 |
| influence of helix pomatia enzyme preparations on the oxidative conversion of some clostebol acetate metabolites in urine. | clostebol acetate (4-chloro-testosterone acetate) is an anabolic steroid used for fattening purposes in cattle breeding. to safeguard public health, its use has been prohibited by the european commission since 1986. screening for its urinary metabolites is therefore an important tool for the control of possible violations. because those metabolites appear conjugated to glucuronic acid or sulfate, deconjugation prior to analysis is necessary. this work describes the variability in results seen wi ... | 2002 | 11916018 |
| [immobilization of immunoglobulins using lectins with an immune sensor method based on surface plasmon resonance]. | the effect of different lectins upon the response of immune sensor based on surface plasmon resonance (spr) was investigated. lectins affinity to carbohydrates of the igg can be used to increase the density and orientation of igg molecules at their immobilisation on the sensor surface. conditions were elaborated for enhancement of immune sensor response in comparison with that one for bare or preliminary treated with dodecanthiol thin gold sensor surface. it was shown that human igg revealed max ... | 2002 | 12152312 |
| study of natural and artificial corticosteroid phase ii metabolites in bovine urine using hplc-ms/ms. | corticosteroid compounds are widely used therapeutically for their anti-inflammatory properties and sometimes as growth promoters in food producing animals. in the field of drug residue analysis, knowledge of the main metabolic pathways of target analytes improves the efficiency of the corresponding control. thus, phase ii metabolism of corticosteroids, for which very little literature is available, was investigated in cattle. an lc-ms/ms detection method was developed for five commercially avai ... | 2002 | 12231123 |
| some new aspects of 17alpha-estradiol metabolism in man. | 17alpha-estradiol (1,3,5(10)-estratriene-3,17alpha-diol) together with a tracer dose of the tritium-labeled compound was administered orally and sublingually to male volunteers. the serum concentrations of 17alpha-estradiol (free and liberated by enzymatic hydrolysis) were quantified by gc/ms, and the serum total radioactivity and urinary radioactivity excretion were determined. after oral administration, 17alpha-estradiol was rapidly and intensively conjugated; only tiny quantities of the free ... | 2002 | 12234624 |
| investigations into the potential role of aberrant n-acetylgalactosamine glycans in tumour cell interactions with basement membrane components. | the expression of the aberrant n-acetylgalactosamine (galnac) glycoconjugates, detected by binding of the lectin from helix pomatia (hpa) is reported to be associated with metastatic competence and poor prognosis in a range of human adenocarcinomas, but the functional significance of the glycoconjugates in metastatic mechanisms is unknown. we have employed seven cell lines derived from normal breast epithelium, primary breast cancer and breast cancer metastases which stably express varying level ... | 2002 | 12405285 |
| cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding. | gastric gland component cells were electron-microscopically and immunoelectronmicroscopically examined with high-pressure freezing followed by freeze substitution and a low-temperature embedding resin method and compared to that of the conventional chemical-fixation method. the rat gastric gland was high-pressure frozen, freeze-substituted with acetone-containing osmium or acrolein, and embedded in epon 812 or lowicryl k4m, respectively. using the high-pressure freezing method, the vitreous free ... | 2002 | 12418087 |
| conformational analysis of two xylose-containing n-glycans in aqueous solution by using 1h nmr roesy and noesy spectroscopy in combination with md simulations. | the conformational behavior of the synthetic hexa- and heptasaccharide methyl beta-glycosides alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-beta-d-glcpnac-(1 --> ome and alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-[alpha-l-fucp-(1 --> 6)-]beta-d-glcpnac-(1 --> ome, representing the xylosylated and the xylosylated alpha-(1 --> 6)-fucosylated core stru ... | 2002 | 12433493 |
| preparation of gc protein-derived macrophage activating factor (gcmaf) and its structural characterization and biological activities. | gc protein has been reported to be a precursor of gc protein-derived macrophage activation factor (gcmaf) in the inflammation-primed macrophage activation cascade. an inducible beta-galactosidase of b cells and neuraminidase of t cells convert gc protein to gcmaf. | 2002 | 12553073 |
| changes in the distribution of sugar receptors in blood vessels and cell elements of rat brain caused by photochemically induced focal ischaemia. | the lectin technique was applied to the histochemical studies of glycoconjugates comprising membranes of capillary endothelial cells and other cellular elements of the cns. in the studies we used the following lectins: concanavalin a (con a), bandeirea simplicifolia agglutinin (bs-i), helix pomatia agglutinin (hpa), ricinus communis agglutinin (rca-120), wheat germ agglutinin (wga). we noticed changes in the localisation of the glycoconjugates, which are the result of cerebral ischaemia caused b ... | 2002 | 12572776 |
| screening of clostebol and its metabolites in bovine urine with elisa and comparison with gc-ms results in an interlaboratory study. | an extraction procedure for clostebol metabolites in urine is developed including enzymatic hydrolysis of conjugated metabolites with helix pomatia juice (shp) and solid-phase extraction (spe) with further cleanup of sample extracts. for the enzymatic deconjugation step, variables such as buffer ph, amount of enzyme, incubation time, and temperature are examined. for the spe step, different wash solutions and combinations with subsequent liquid-liquid extractions are examined. incurred bovine ur ... | 2003 | 12820743 |
| the role of calcium in mucin packaging within goblet cells. | recent reports hypothesize that calcium plays an important role in providing cationic shielding to keep negatively charged mucins condensed and tightly packed within mucus granules of goblet cells. vitamin d controls mineral ion homeostasis and intestinal calcium absorption, which is mediated by the nuclear vitamin d receptor (vdr). hypocalcemia is observed in mice in which the vdr has been ablated. the purpose of this study was to test the hypothesis that normal levels of calcium are required f ... | 2003 | 12823989 |
| hemocyte-mediated phagocytosis and melanization in the mosquito armigeres subalbatus following immune challenge by bacteria. | mosquitoes are important vectors of disease. these insects respond to invading organisms with strong cellular and humoral immune responses that share many similarities with vertebrate immune systems. the strength and specificity of these responses are directly correlated to a mosquito's ability to transmit disease. in the current study, we characterized the hemocytes (blood cells) of armigeres subalbatus by morphology (ultrastructure), lectin binding, enzyme activity, immunocytochemistry, and fu ... | 2003 | 12838409 |
| [biological assay of preparations of helix pomatia l..]. | | 1954 | 13189202 |
| validation of a simple avidin-biotin detection method for helix pomatia lectin (hpa) binding as a prognostic marker in cancer. | helix pomatia lectin (hpa) binding is a marker of metastatic competence in several human cancers. the altered cellular glycosylation detected by hpa is of clinical interest and functional significance, but research has been hampered by uncertainty over appropriate and accessible histochemical methods. most studies have employed a complex multi-layered detection system localising binding of unconjugated hpa by layering with a polyclonal antibody to hpa, a biotinylated secondary antibody against t ... | 2003 | 13677613 |
| the comparative ability of beta-glucuronidase preparations (liver, escherichia coli, helix pomatia, and patella vulgata) to hydrolyze certain steroid glucosiduronic acids. | | 1961 | 13782588 |
| spheroplasts of the yeast candida utilis. | svihla, g. (argonne national laboratory, argonne, ill.), f. schlenk, and j. l. dainko. spheroplasts of the yeast candida utilis. j. bacteriol. 82:808-814. 1961.-the formation and properties of spheroplasts of the yeast candida utilis, produced by digestion of the cell walls with snail gut juice (helix pomatia) in isotonic medium, were studied by phase, interference, and ultraviolet microscopy, and tracer techniques. cells cultivated in the presence of l-methionine, s-methyl-l-methionine, and l-h ... | 1961 | 13918716 |
| a comparison of hydrolase and lytic activities of the digestive juices of the giant african snail, achatina fulica, and helix pomatia. | | 1963 | 13981006 |
| some cytological and pathogenic properties of spheroplasts of candida albicans. | kobayashi, george s. (tulane university, new orleans, la.), lorraine friedman, and judith f. kofroth. some cytological and pathogenic properties of spheroplasts of candida albicans. j. bacteriol. 88:795-801. 1964.-spheroplasts of candida albicans were prepared by use of an enzymatic mixture from the digestive tract of the snail helix pomatia. untreated cells exhibited well-defined cell walls, whereas such structures were absent from spheroplasts. the intravenous inoculation of either spheroplast ... | 1964 | 14208520 |
| [studies on the acetylcholinesterase in the blood of helix pomatia. 3. a combined optical test for determining the enzyme activity]. | | 1964 | 14236172 |
| beta-galactosidase of helix pomatia. | | 1964 | 14236292 |
| urinary excretion of c-20-reduction products of corticosterone. | 1. a 200 mg. portion of corticosterone was ingested by a healthy man and the urine collected. part of the urine was treated with the gastric juice of helix pomatia and extracted with ethyl acetate, and the extract fractionated with girard t. paper-chromatographic separation of the non-ketonic fraction in the bush (1952) system b(5) revealed the presence of two unknown polar components. 2. the unknown compounds did not possess a reducing (blue tetrazolium) or a reducible (potassium borohydride) g ... | 1965 | 14342242 |
| expression of cpgp40/15 in toxoplasma gondii: a surrogate system for the study of cryptosporidium glycoprotein antigens. | cryptosporidium parvum is a waterborne enteric coccidian that causes diarrheal disease in a wide range of hosts. development of successful therapies is hampered by the inability to culture the parasite and the lack of a transfection system for genetic manipulation. the glycoprotein products of the cpgp40/15 gene, gp40 and gp15, are involved in c. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies. however, the function o ... | 2003 | 14500524 |
| effect of sugars on the association between cowpea vicilin (7s storage proteins) and fungal cells. | vicilins (7s storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. in the present work vicilins from cowpea (vigna unguiculata) seeds were added to the growth medium of saccharomyces cerevisiae cells and fusarium oxysporum conidia. helix pomatia lectin, wheat germ agglutinin and ulex europaeus lectin were ... | 2003 | 14510235 |
| optimized glucuronide hydrolysis for the detection of psilocin in human urine samples. | in order to develop a sensitive and reliable analytical method for psilocin (pc) in urine samples, the hydrolysis conditions including the acid, alkaline and enzymatic hydrolyses have been investigated by monitoring not only pc but also psilocin glucuronide (pcg) by liquid chromatography tandem mass spectrometry (lc-ms-ms); pcg was initially identified in a "magic mushroom (mm)" user's urine by liquid chromatography mass spectrometry (lc-ms) and lc-ms-ms. the proposed conditions optimized for th ... | 2003 | 14581081 |
| the parasitic trematode fasciola hepatica exhibits mammalian-type glycolipids as well as gal(beta1-6)gal-terminating glycolipids that account for cestode serological cross-reactivity. | neutral glycosphingolipids from sheep-derived fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. after hplc fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and maldi-tof as well as electrospray ionization mass spectrometry. obtained results revealed the presence of two types of neutral glycolipids. the first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (gal(alpha1-4)gal( ... | 2004 | 14638629 |
| metabolism of the soy isoflavones daidzein, genistein and glycitein in human subjects. identification of new metabolites having an intact isoflavonoid skeleton. | epidemiological studies have associated high soy intake with a lowered risk for certain hormone-dependent diseases. soy and soy foods are rich sources of isoflavones, which have been shown to possess several biological activities. in this study, the metabolism of soy isoflavones daidzein, genistein and glycitein was investigated in human subjects. the aim was to find and identify urinary phase i metabolites of isoflavones, which have an intact isoflavonoid skeleton, and which might possess some ... | 2003 | 14698210 |
| leptomonas wallacei shows distinct morphology and surface carbohydrates composition along the intestinal tract of its host oncopeltus fasciatus (hemiptera: lygaeidae) and in axenic culture. | leptomonas wallacei is a monoxenic trypanosomatid that colonizes the digestive tract of the phytophagous hemipteran oncopeltus fasciatus. this infection was specific and took place exclusively in midgut intestinal ventricles v3 and v4, and in the hindgut. abundances of parasites in the hindgut were 54% less than those in the hindgut. parasites in the hindgut were more slender and had a longer flagellum than those from the hindgut, which were rounded, with a shorter flagellum. moreover, hindgut f ... | 2003 | 14974433 |
| exposed and hidden lectin-binding epitopes at the surface of borrelia burgdorferi. | the presence of surface- and subsurface-located lectin-binding epitopes of borrelia burgdorferi was examined by electron microscopy using a variety of gold-labeled lectins. concanavalin a reacted predominantly with extracellular material adjacent to the spirochetes. wheat germ agglutinin bound weakly to the surface of borreliae; however, alterations of the outer membrane by preincubation in 100 ppm triton x-100 or boiling uncovered numerous periplasmic sites recognized by the lectin. the peripla ... | 2003 | 14976724 |
| monoclonal antibodies against the tn-specific isolectin b4 from vicia villosa seeds: characterization of the epitope of the blocking antibody vv34. | vicia villosa isolectin b4 (vvlb4) recognizes the tn antigen (galnac-o-ser/thr) exposed in certain human carcinomas. we have produced anti-vvlb4 monoclonal antibodies (mabs), and their lectin recognition selectivity was assessed by elisa and western blot against the purified gal/galnac-specific lectins from vicia villosa, salvia sclarea, helix pomatia, arachis hypogaea, glycine max, and dolichos biflorus. the antibodies were also tested for their ability to block the binding of vvlb4 to the tn a ... | 2004 | 15000847 |
| structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. | lung macro- and microvascular endothelial cells exhibit unique functional attributes, including signal transduction and barrier properties. we therefore sought to identify structural and functional features of endothelial cells that discriminate their phenotypes in the fully differentiated lung. rat lung macro- (paec) and microvascular (pmvec) endothelial cells each exhibited expression of typical markers. screening for reactivity with nine different lectins revealed that glycine max and griffon ... | 2004 | 15020205 |
| use of taxol and collagenase for better three-dimensional visualization of microtubules in the enterocyte and brunner's gland cell, with special reference to their relation to the golgi apparatus. | cytoskeletal microtubules were visualized in the mouse duodenal mucosa by an improved immunofluorescence method using a microtubule-stabilizing reagent, taxol, and collagenase as an enzymatic epitope retriever. the improvement in immunostaining was shown morphologically and statistically by comparing fluorescence intensities of specimens prepared with or without taxol and collagenase treatment. in free cells in the epithelium and in the lamina propria, microtubules radiated from the gamma-tubuli ... | 2004 | 15077902 |
| amide n-glucuronidation of maxipost catalyzed by udp-glucuronosyltransferase 2b7 in humans. | maxipost [(3s)-(+)-(5-chloro-2-methoxyphenyl)-1,3-dihydro-3-fluoro-6-(trifluoromethyl)-2h-indole-2-one), or bms-204352)] is a potent and specific maxi-k channel opener for potential use to treat stroke. this article describes structural characterization of a major human n-glucuronide metabolite of bms-204352 and identification of the enzyme responsible for the n-glucuronidation reaction. after intravenous administrations of [(14)c]bms-204352 (10 mg, 50 microci) to eight healthy human subjects, o ... | 2004 | 15100177 |
| ability of blood group a-active glycosphingolipids to act as escherichia coli heat-labile enterotoxin receptors in ht-29 cells. | we examined the ability of blood group a-active glycoconjugates to act as receptors for escherichia coli heat-labile type i enterotoxin (lt-i) in ht-29 cells. these cells contained ~4 times more specific binding sites for lt-i than for cholera toxin (ct). binding of lt-i could not be blocked by the b subunit of ct (ct-b), indicating the existence of lt-i receptors in addition to the glycosphingolipid gm1. lt-i was able to increase levels of cyclic adenosine monophosphate (amp), even in the prese ... | 2004 | 15116290 |
| herpes simplex virus type-2 specific glycoprotein g-2 immunomagnetically captured from hep-2 infected tissue culture extracts. | monoclonal antibody h1206 anti-hsv-2 gg-2 bound to tosylactivated paramagnetic dynabeads (dynal) has been used to isolate hsv-2 type-specific gg-2 from solubilized hep-2 hsv-2 infected cell extracts. the immunomagnetically captured type-specific glycoprotein reacted strongly with monoclonal antibody h1206 and demonstrated a single band with apparent molecular weight of 100000 (100 kda) and a doublet band with an apparent molecular weight of 60000-64000 (60-64 kda). we observed the same exact ban ... | 2004 | 15158593 |
| in vitro capacitation and acrosome reaction of dog spermatozoa can be feasibly attained in a defined medium without glucose. | incubation of dog spermatozoa in a medium without glucose and in the presence of lactate and pyruvate (l-ccm) for 4 h at 38.5 degrees c in a 5% co(2) atmosphere induced in vitro capacitation of these cells. this was verified after the combined specific capacitation-like changes in percentages of viability and altered acrosomes, motility characteristics, sperm location of reactivity against pisum sativum, arachis hypogaea and helix pomatia lectins and the tyrosine phosphorylation pattern. further ... | 2004 | 15182287 |
| sperm binding to oviductal epithelial cells in the rat: role of sialic acid residues on the epithelial surface and sialic acid-binding sites on the sperm surface. | the aim of this study was to assess the participation of carbohydrate residues in the adhesion of spermatozoa to the oviductal epithelium in the rat. we first examined, by lectin labeling, the distribution of glycoconjugates in rat oviducts obtained under various hormonal environments. several classes of glycoconjugates were abundant in the epithelium, and the expression of some of these molecules varied differentially in ampulla and isthmus, along the estrous cycle and with estradiol and proges ... | 2004 | 15201197 |
| epileptogenicity and epileptic activity: mechanisms in an invertebrate model nervous system. | epileptic seizures are based on paroxysmal depolarization shifts (pds) which are synchronized in many neurons. mechanisms underlying pds and seizures are still not understood. the present review is based on studies using the buccal ganglia of the snail helix pomatia as a model nervous system. essential mechanisms of epileptic activity in nervous systems are thought to be identical in whatever part of the human or animal nervous system epileptic activity appears. from studies using the buccal gan ... | 2004 | 15216913 |
| functional morphology of the salivary gland of the snail, helix pomatia: a histochemical and immunocytochemical study. | functional morphology of helix pomatia salivary gland cells was studied at light microscopic level by using different histochemical methods. three cell types could be demonstrated in the salivary gland: mucocytes, granular and vacuolated cells. the distribution and the number of the different cell types were different in active and inactive snails. in active feeding animals, dilatated interlobular salivary ducts were observed, which were never present in inactive ones. in active animals an addit ... | 2004 | 15270238 |
| continuous increase of epileptogenic effects following application of proteolytic enzymes (buccal ganglia of helix pomatia). | epileptic activity of neurons consists of paroxysmal depolarization shifts (pds) which can be induced presumably in any nervous system by application of an epileptogenic drug. the spontaneous appearance of epileptic activity, however, is based on a largely unknown process which increases susceptibility to epileptic activity (seizure susceptibility in man). it is presently shown that the treatment of ganglia with proteolytic enzymes (pronase) decreases the effective concentration of epileptogenic ... | 2004 | 15270243 |
| helix pomatia a hemagglutinin, a surface marker for bovine t-lymphocytes. | bovine peripheral blood lymphocytes (pbl) were isolated from blood collected from 6 cattle. after treatment with neuraminidase, 40 or 60% of the cells were shown to combine with helix pomatia a hemagglutinin (hp) depending whether a direct or indirect fluorescence technique was used. about 20% of the cells were ig-bearing. with double staining fluorescence technique, it was shown that cells attaching to hp were not ig-bearing and the reverse. with the aid of hp, covalently bound to sepharose, ig ... | 1979 | 15612267 |