| modes of entry of the first-stage larvae of elaphostrongylus cervi (nematoda: protostrongylidae) into pulmonate snails arianta arbustorum and helix pomatia. | experimental infection of the pulmonate snails arianta arbustorum l. and helix pomatia l. with first-stage larvae of protostrongylid nematode elaphostrongylus cervi cameron, 1931 was performed in order to determine modes of larval entry into the body of the snail intermediate host. groups by four individuals of both snail species were examined histologically 30, 60, 90, and 120 minutes after the beginning of exposure and 1, 2, 4, and 7 days post infection. all 64 snails examined were found to be ... | 1994 | 7883253 |
| lectin binding in the anterior segment of the bovine eye. | eleven different fluorescent lectin-conjugates were used to reveal the location of carbohydrate residues in frozen sections of the anterior segment of bovine eyes. the lectins were specific for the following five major carbohydrate groups: (1) glucose/mannose group (concanavalin a (con a)); (2) n-acetylglucosamine group (wheat germ agglutinin (wga)); (3) galactose/n-acetylgalactosamine group (dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), helix aspersa agglutinin (haa), psop ... | 1994 | 7883589 |
| glycosylation patterns of the human colon cancer cell line ht-29 detected by helix pomatia agglutinin and other lectins in culture, in primary tumours and in metastases in scid mice. | human colonic cancer cells (ht-29, 10(7) cells/dose) were injected subcutaneously between the scapulae of 19 severe combined immunodeficient (scid) mice. after 19 days, large tumours had developed in 18 out of the 19 animals and the mice were then killed. metastases were detected in the lungs of 16 animals but not in other organs investigated. surgical removal of the primary tumour in another group of five animals led to a prolonged survival and further growth of metastases in the lungs. ht-29 i ... | 1994 | 7923992 |
| interference of helix pomatia extracts on the determination of methandriol in veterinary residue control analysis. | aqueous solutions containing methandriol (mad) were incubated with succus helix pomatia (shp) or beta-glucuronidase from escherichia coli (ec). shp, used for enzymatic hydrolysis of urinary steroid conjugates for residue analysis of anabolic agents, caused transformation of mad into methyltestosterone. no conversion occurred when bacterial beta-glucuronidase from e. coli was used. | 1998 | 10435316 |
| galactose-deficient iga1 in sera of iga nephropathy patients is present in complexes with igg. | iga1 proteins from sera of patients with iga nephropathy (igan) are galactosylated to a lesser degree than those from healthy controls. the increased reactivity of intact or de-sialylated serum iga1 with n-acetylgalactosamine (galnac)-specific lectins, helix aspersa (haa) and caragana arborescens (caa) and de-sialylated iga1 with helix pomatia (hpa) and bauhinia purpurea (bpa) indicated that the gal deficiency is in glycans located in the hinge region of iga1 molecules. de-sialylated iga from se ... | 1997 | 9264010 |
| [caragana arborescens lectin--a marker of myoepitheliocytes of salivary glands]. | submaxillary salivary gland preparations of a horse, a cow, a pig, a rabbit and white outbred rats were stained with peroxidase-labeled lectins of different carbohydrate specificity. it was found that n-acetyl-d-galactosamine-specific lectins (caragana arborescens lam., phaseolus unatus seeds, helix pomatia sails) bind selectively with myoepitheliocytes. best marker of the latter proved to be caragana arborescens lectin, consisting of two components which differ in their affinity to n-acetyl-d-g ... | 1999 | 10486811 |
| localization of procollagen i in the lysosome/endosome system of human fibroblasts. | a significant amount of newly synthesized collagen is degraded intracellularly rather than secreted, but there is controversy about whether this process occurs in the lysosomes. we addressed this problem using confocal microscopy and immunofluorescence imaging to study the distribution of procollagen i in the golgi and the lysosome/endosome system of cultured human fibroblasts. cells were incubated under basal conditions and then permeabilized and exposed to fluorescently tagged probes for proco ... | 1997 | 9344594 |
| primary structure of 21 novel monoantennary and diantennary n-linked carbohydrate chains from alphad-hemocyanin of helix pomatia. | the primary structures of 21 novel monoantennary and diantennary n-glycans of the glycoprotein alphad-hemocyanin (alphad-hc) of helix pomatia have been determined. outer oligosaccharide fragments (antennae) were released from the glycoprotein by smith degradation of an alphad-hc pronase digest. the major antenna, obtained following hplc fractionation on lichrosorb-nh2, was characterized using 1h-nmr spectroscopy, fast-atom-bombardment mass spectrometry, and linkage analysis, and corresponds to a ... | 1997 | 9363772 |
| development of a simple method for the determination of genistein, daidzein, biochanin a, and formononetin (biochanin b) in human urine. | a simple method was developed for the determination of free and/or total isoflavones daidzein, genistein, and their respective 4'-methoxy derivatives biochanin a and formononetin (biochanin b) at low levels in human urine. a solid-phase extraction on octadecyl silica (c(18)) columns was used for the isolation of the phytoestrogens from the matrix. an extraction on a chemelut 1010 column connected on-line to a florisil cartridge by a teflon stopcock was used for effective eluate purification. a m ... | 1999 | 10552674 |
| the rational design of semisynthetic peroxidases. | a semisynthetic peroxidase was designed by exploiting the structural similarity of the active sites of vanadium dependent haloperoxidases and acid phosphatases. incorporation of vanadate ion into the active site of phytase (e.c. 3.1.3.8), which mediates in vivo the hydrolysis of phosphate esters, leads to the formation of a semisynthetic peroxidase, which catalyzes the enantioselective oxidation of prochiral sulfides with h(2)o(2) affording the s-sulfoxide, e.g. in 66% ee at 100% conversion for ... | 2000 | 10581439 |
| lectins and antibodies to blood group antigens as markers for the basal cells of the human respiratory epithelium. | we used a pattern of 30 lectins and antibodies against antigens of the abo-blood group system to find specific and sensitive markers for the basal cells of the human respiratory surface epithelium. three lectins always stained the basal cells: aaptos papillata agglutinin i (apa i), peanut agglutinin (pna), and wheat germ agglutinin (wga): other lectins and the antibodies gave positive results only in tissue of secretors (blood group antigens in secretions) and these were dependent on the abo-blo ... | 1997 | 9376654 |
| lectin histochemistry of the rat brain following thioacetamide-induced hepatic failure. | biotinyl derivatives of several lectins were used to study the localization of glycoconjugates in the cerebral microcapillaries and various brains of rats given at 24-h intervals two i.p. administrations of a hepatotoxin-thioacetamide (taa) and examined 21 d posttreatment. at this time, the rats were asymptomatic with regard to hepatic encephalopathy but showed specific and selective changes in the blood-brain-barrier (bbb) transport of basic amino acid, but no bbb damage, and region-specific ne ... | 1997 | 9437665 |
| electrospray ionization mass spectrometry of zinc, cadmium, and copper metallothioneins: evidence for metal-binding cooperativity. | electrospray ionization (esi) mass spectra of both well-characterized and novel metallothioneins (mts) from various species were recorded to explore their metal-ion-binding modes and stoichiometries. the esi mass spectra of the zinc- and cadmium-binding mts showed a single main peak corresponding to metal-to-protein ratios of 4, 6, or 7. these findings combined with data obtained by other methods suggest that these mts bind zinc or cadmium in a single predominant form and are consistent with the ... | 2000 | 10716192 |
| invasion potential and n-acetylgalactosamine expression in a human melanoma model. | reactivity of the n-acetylgalactosamine-binding helix pomatia agglutinin (hpa) in tumours has been associated with poor prognosis and metastasis development. in our lox/femx-i human melanoma model, the binding of hpa correlates with experimental lung metastasis formation in athymic nude mice. in the present study, the metastatic potential of 2 human melanoma cell lines (lox and femx-i) was assessed in relation to carbohydrate and invasive phenotype. immunocytological and invasion assays highligh ... | 1998 | 9466664 |
| core alpha1,3-fucose is a key part of the epitope recognized by antibodies reacting against plant n-linked oligosaccharides and is present in a wide variety of plant extracts. | carbohydrates have been suggested to account for some ige cross-reactions between various plant, insect, and mollusk extracts, while some igg antibodies have been successfully raised against plant glycoproteins. a rat monoclonal antibody raised against elderberry abscission tissue (yz1/2.23) and rabbit polyclonal antiserum against horseradish peroxidase were screened for reactivity in enzyme-linked immunosorbent assay against a range of plant glycoproteins and extracts as well as neoglycoprotein ... | 1998 | 9621106 |
| histological disorders related to the focal disappearance of the epiphyseal growth plate in rats induced by high dose of vitamin a. | the histological disorders related to the focal disappearance of the epiphyseal growth plate were examined histochemically in the proximal tibia of rats administered a high dose of vitamin a. animals were given 100,000 iu/100 g body weight/day of vitamin a for 5 days from 4 weeks after birth (va rats) or given deionized water as control and sacrificed on day 12 and 19 of the experiment. tibiae were examined by immunohistochemistry for type i, ii and x collagens, lectin-histochemistry for helix p ... | 2000 | 10770602 |
| characterisation of lectin binding patterns of mouse bronchiolar and rat alveolar epithelial cells in culture. | lung epithelial cell differentiation pathways remain unclear. this is due in part to the plasticity of these cells and the lack of markers which accurately reflect their differentiation status. the aim of this study was to determine if lectin binding properties are useful determinants of functional differentiation status in vitro. mouse clara cells were cultured for 5 days. during this time, no alteration in differentiation was evident by electron microscopy. no significant alteration in binding ... | 2000 | 10805383 |
| stability and enzymatic hydrolysis of quaternary ammonium-linked glucuronide metabolites of drugs with an aliphatic tertiary amine-implications for analysis. | quaternary ammonium-linked glucuronide (n+-glucuronide) metabolites formed at aliphatic tertiary amine functional groups of xenobiotics have not been previously systematically studied with respect to their stability over a wide ph range and the ease of enzymatic hydrolysis by beta-glucuronidase from various sources. three and four n+-glucuronide metabolites were respectively studied regarding their non-enzymatic and enzymatic stabilities where the metabolites were quantified by hplc procedures. ... | 2000 | 10815723 |
| development of conjunctival goblet cells and their neuroreceptor subtype expression. | to investigate expression of muscarinic, cholinergic, and adrenergic receptors on developing conjunctival goblet cells. | 2000 | 10892854 |
| detection of inflammation- and neoplasia-associated alterations in human large intestine using plant/invertebrate lectins, galectin-1 and neoglycoproteins. | commonly, plant and invertebrate lectins are accepted glycohistochemical tools for the analysis of normal and altered structures of glycans in histology and pathology. mammalian lectins and neoglycoproteins are recent additions to this panel for the detection of lectin-reactive carbohydrate epitopes and glycoligand-binding sites. the binding profiles of these three types of probes were comparatively analyzed in normal, inflamed and neoplastic large intestine. in normal colonic mucosa the intrace ... | 1998 | 9780361 |
| localized infusion of tunicamycin in rat hemimandibles: alteration of the basal lamina associated with maturation stage ameloblasts. | at the beginning of the maturation stage of amelogenesis, ameloblasts deposit a basal lamina (bl) at the interface between their apical surface and maturing enamel. this structure is rich in glycoconjugates and is proposed to exhibit adhesive and/or filtering functions. to clarify its role, we have applied a recently developed surgical window model to locally administer tunicamycin (tm), an antibiotic that interferes with n-glycosylation, in the rat hemimandible using an osmotic minipump. male w ... | 2001 | 11156685 |
| analysis of glycoconjugate patterns of normal and hormone-induced dysplastic noble rat prostates, and an androgen-independent noble rat prostate tumor, by lectin histochemistry and protein blotting. | alteration of the expression of glycoconjugates is frequently observed in tumors. however, studies on the changes of cellular glycosylation in the early premalignant stage of prostate carcinogenesis are scarce. | 2001 | 11170128 |
| novel forms of protein glycosylation. | a large number of new glycans, derived from glycoproteins, has been characterized in the past few years. o-linked fucose was found in epidermal growth factor-like domains of several proteins. for the n-linked glycans of helix pomatia hemocyanin, novel types of antennae were identified. the positions of noncarbohydrate substituents were established in n-glycans. c-mannosylation of a tryptophan residue was discovered in human ribonuclease 2 and is the first example of c-glycosylation in glycoprote ... | 1998 | 9818259 |
| the use of the lectin helix pomatia agglutinin (hpa) as a prognostic indicator and as a tool in cancer research. | progress in treatment for cancer has enabled extension of the disease-free interval, and of the quality of life for patients, but there has been very little improvement in overall survival rates. the main reason for this has been the ineffectiveness of current therapies to kill all the cancer cells once they have spread to distant sites to form metastatic deposits. one marker which has proved to be useful in identifying those cancers which have the potential to spread is the lectin helix pomatia ... | 1999 | 9987666 |
| [the bronchorelaxant effect of helicidine, a helix pomatia extract, interferes with prostaglandin e2]. | helicidine is a biological extract prepared from the snail helix pomatia l. and used in man as an anti-tussive agent. however, its mechanisms of action are not fully defined. in this study, we have investigated a possible relaxant effect of helicidine on guinea-pig airway smooth muscle and evaluated the role of prostanoids and airway epithelium in this relaxation. h. pomatia extract (0.001-1 mg/ml) induced a dose-dependent relaxation of guinea-pig trachea pre-contracted with histamine both in th ... | 1999 | 10081783 |
| comparative lectin-histochemistry on the pre-epithelial mucus layer in the distal colon of conventional and germ-free rats. | the mucin composition of the rat distal colonic pre-epithelial mucus layer (pml) was studied by lectin histochemistry in conventional (cv), and germ-free (gf) rats to define effects exerted by the gut flora. no peanut agglutinin (pna) binding was observed in the pml of gf rats, while the pml of their cv counterparts showed a considerable pna linkage, indicating terminal gal-beta1,3-galnac residues. soybean agglutinin (sba) and helix pomatia agglutinin (hpa) stained the pml mucins in cv and in gf ... | 1998 | 11253810 |
| binding properties of a blood group le(a+) active sialoglycoprotein, purified from human ovarian cyst, with applied lectins. | studies on the structures and binding properties of the glycoproteins, purified from human ovarian cyst fluids, will aid the understanding of the carbohydrate alterations occurring during the biosynthesis of blood group antigens and neoplasm formation. these glycoproteins can also serve as important biological materials to study blood group a, b, h, le(a), le(b), le(x), le(y), t and tn determinants, precursor type i and ii sequences and cold agglutinin i and i epitopes. in this study, the bindin ... | 1996 | 8672551 |
| a new subgroup of lectin-bound biliary proteins binds to cholesterol crystals, modifies crystal morphology, and inhibits cholesterol crystallization. | biliary proteins inhibiting or promoting cholesterol crystallization are assumed to play a major role in cholesterol gallstone pathogenesis. we now report a new group of biliary proteins that bind to cholesterol crystals, modify crystal morphology, and inhibit cholesterol crystallization. various glycoprotein mixtures were extracted from abnormal human gallbladder bile using lectin affinity chromatography on concanavalin a, lentil, and helix pomatia columns and were added to supersaturated model ... | 1995 | 8675674 |
| identification of beta-glucuronidase-resistant diastereomeric glucuronides of 3-hydroxy-3-methyloxindole formed during 3-methylindole metabolism in goats. | goats were jugularly infused with the pneumotoxin 3-methylindole (3mi; 15 mg/kg, 0.5 microci/kg) dissolved in cremophor-el to characterize the urinary metabolites of 3mi in a ruminant specie. urine was collected for 36 hr after the beginning of a 2-hr infusion period, and 3mi metabolites were purified using reversed-phase hplc. goats excreted 3mi as at least 11 distinct metabolites. metabolites were characterized using a combination of uv spectroscopy, 1h- and 13c-nmr spectroscopy, and negative- ... | 1996 | 8825199 |
| lectin binding patterns of uterine glands in mares with chronic endometrial degeneration. | to evaluate changes of glycoconjugate in uterine glands of endometrial tissues obtained from mares. | 2001 | 11400838 |
| suitability of different staining methods for the identification of isolated and cultured cells from guinea pig (cavia aperea porcellus) stomach. | cell suspensions from the guinea pig gastric mucosa were obtained using a pronase/collagenase isolation method, and cultured on petri dishes in minimum essential medium at 37 degrees c. for proper identification of different gastric cell types in cytospots, cell suspensions or culture, selective staining methods were employed, modified and evaluated. mucous cells and mucous neck cells were detected by use of lectins. mucous cells were stained on cytospots and in primary cultures with lectins fro ... | 1995 | 8835136 |
| hydrolysis of conjugated metabolites of buprenorphine. i. the quantitative enzymatic hydrolysis of buprenorphine-3-beta-d-glucuronide in human urine. | buprenorphine, which is a powerful analgesic, a substitution drug for opioids widely used in europe, and a promising new drug currently undergoing clinical trials in the treatment of opioid dependence in the u.s., is excreted in human urine mainly as glucuronide conjugates. in gas chromatographic-mass spectrometric analysis, the urine specimens must be first hydrolyzed to release buprenorphine from its glucuronide conjugates. in order to evaluate the existing hydrolysis methods and to find the o ... | 2001 | 11599605 |
| proteome and glycosylation mapping identifies post-translational modifications associated with aggressive breast cancer. | changes in glycosylation of glycoproteins and glycolipids is a common feature of cancer and may influence cancer cell behaviour, perhaps by enabling cell-cell interactions which favour metastasis or by allowing cancer cells to evade immuno-surveillance. studies to identify glycosylation changes in human cancer have often used immunohistochemical techniques with lectins or antibodies and human tissue sections. whilst some detailed biochemical studies have been performed there are few clinically r ... | 2001 | 11677781 |
| guinea pig intestinal phospholipase b: protein expression during enterocyte maturation and effects of n-oligosaccharide removal on enzymatic activities and protein stability. | guinea pig phospholipase b (plb) is an intestinal brush-border hydrolase displaying a broad substrate specificity towards various dietary lipids. plb was detected by immunoblotting as a single 140-kda polypeptide in all cell populations isolated from guinea pig intestinal mucosa, but increased in parallel to its activity from undifferentiated to mature cells, the specific activity of the enzyme remaining constant. moreover, n-glycosylation, which contributed to 23% of the apparent molecular mass ... | 1996 | 8856041 |
| simultaneous determination of fifteen low-dosed benzodiazepines in human urine by solid-phase extraction and gas chromatography-mass spectrometry. | a gas chromatographic-mass spectrometric method was developed for the simultaneous analysis of 15 low-dosed benzodiazepines, both parent compounds and their corresponding metabolites, in human urine. the target compounds are alprazolam, alpha-hydroxyalprazolam, 4-hydroxyalprazolam, flunitrazepam, 7-aminoflunitrazepam, desmethylflunitrazepam, flurazepam, hydroxyethylflurazepam, nitrogen-desalkylflurazepam, ketazolam, oxazepam, lormetazepam, lorazepam, triazolam and alpha-hydroxytriazolam. nitroge ... | 2001 | 11767312 |
| cloning and sequencing of cdnas encoding for a novel copper-specific metallothionein and two cadmium-inducible metallothioneins from the blue crab callinectes sapidus. | metallothioneins (mts) are cysteine-rich metal-binding proteins found in micro-organisms, plants and all invertebrate and vertebrate animals. unicellular eukaryotes such as yeast have a copper-mt whose synthesis is induced by a copper-activated transcription factor. most higher organisms have two major cadmium/zinc mt isoforms, whose synthesis is controlled by a zinc-activated transcription factor. the blue crab, callinectes sapidus, has two cadmium-inducible isoforms, cdmt-i and cdmt-ii, and a ... | 2000 | 11790353 |
| s100 calcium binding protein affects neuronal electrical discharge activity by modulation of potassium currents. | s100 calcium binding protein has been associated with a variety of intra- and extracellular calcium-mediated functions, including learning and memory. we have previously localized s100-immunoreactive neurons correlated with spontaneous discharge activity in the central nervous system of the mollusc, helix pomatia. in this study, we further investigated the effects of s100 (s100b and s100a1) on electrical discharge activity and membrane currents of helix neurons, using current- and voltage-clamp ... | 1999 | 10215154 |
| [use of estrone sulfate determination in goat blood for the detection of pregnancy and prediction of fetal number]. | the study was carried out on 50 goats of polish white breed. blood samples were taken on day 60 after mating from v. jugularis. pregnancy diagnosis was made using transcutan ultrasound scanning (pie medical scanner 2000 with a 5 mhz curved array probe). the estrone sulphate level was determined by ria method after extraction and hydrolysis with arylsulphatase/glucuronidase from helix pomatia. as a threshold value for differentiation of pregnant from nonpregnant goats 1 ng/ml was assumed. in eigh ... | 1999 | 10326236 |
| intracellular transport and maturation pathway of human herpesvirus 6. | a peculiar characteristic of cells infected with human herpesvirus 6 (hhv6) is the absence of viral glycoproteins on the plasma membrane, which may reflect an atypical intracellular transport of the virions and/or the viral glycoproteins, different from that of the other members of the herpesvirus family. to investigate the maturation pathway of hhv-6 in the human t lymphoid cell line hsb-2, we used lectin cytochemistry and immunogold labeling combined with several electron microscopical techniq ... | 1999 | 10329556 |
| enzyme immunoassay for the measurement of 17alpha-estradiol 17-n-acetylglucosaminide in rabbit urine. | 17alpha-estradiol 17-n-acetylglucosaminide (17alphae2 17nag) is an estrogen metabolite hitherto obtained only in rabbits. to gain insight into this unique conjugate, an enzyme immunoassay (eia) was established by using antiserum elicited against 3-[3-(1-carboxypropyl)] ether of 17alphae2 17nag-bovine serum albumin conjugate; horseradish peroxidase, as a label; and 3,3',5,5'-tetramethylbenzidine, as a chromogen. the method proved to be specific, and the detection range of the assay was 0.20-10.00 ... | 1999 | 10399888 |
| determination of oestrogen concentrations in bovine plasma by a recombinant oestrogen receptor-reporter gene yeast bioassay. | a recombinant cell yeast bioassay (rcba) was applied to the generic measurement of bovine plasma oestrogen concentration. samples were prepared by diethyl ether extraction of plasma following addition of [3h]17 beta-oestradiol as internal standard; organic and aqueous phases were separated by freezing (recovery 97.1 +/- 0.7%) and dried extract reconstituted in culture medium (recovery 31.4 +/- 4.5%). plasma oestrogen concentrations were measured by incubation of extracts with yeast containing a ... | 1998 | 10435304 |
| cytochemical investigation of gastric gland component cells with high-pressure freezing followed by freeze-substitution and hydrophilic resin embedding. | gastric gland component cells were electron-microscopically and immunoelectronmicroscopically examined with high-pressure freezing followed by freeze substitution and a low-temperature embedding resin method and compared to that of the conventional chemical-fixation method. the rat gastric gland was high-pressure frozen, freeze-substituted with acetone-containing osmium or acrolein, and embedded in epon 812 or lowicryl k4m, respectively. using the high-pressure freezing method, the vitreous free ... | 2002 | 12418087 |
| conformational analysis of two xylose-containing n-glycans in aqueous solution by using 1h nmr roesy and noesy spectroscopy in combination with md simulations. | the conformational behavior of the synthetic hexa- and heptasaccharide methyl beta-glycosides alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-beta-d-glcpnac-(1 --> ome and alpha-d-manp-(1 --> 6)-[alpha-d-manp-(1 --> 3)-][beta-d-xylp-(1 --> 2)-]beta-d-manp-(1 --> 4)-beta-d-glcpnac-(1 --> 4)-[alpha-l-fucp-(1 --> 6)-]beta-d-glcpnac-(1 --> ome, representing the xylosylated and the xylosylated alpha-(1 --> 6)-fucosylated core stru ... | 2002 | 12433493 |
| utility of isolated hepatocytes and radio-hplc-msn for the analysis of the metabolic fate of 19-nortestosterone laurate in cattle. | the metabolic fate of 19-nortestosterone laurate in cattle was investigated to evaluate target analyte(s) appropriate to surveillance for illicit use as a growth promoting agent. bovine hepatocytes were incubated with either [3h]19-nortestosterone laurate (19-ntl; 4-estren-17 beta-laurate-3-one) or [3h]19-nortestosterone (19-nt; 4-estren-17 beta-ol-3-one; nandrolone). hepatocyte medium was extracted with solid phase c18 media and analysed by narrow bore radio-hplc-msn (lcq, finnigan) to evaluate ... | 1998 | 10435305 |
| changes in the distribution of sugar receptors in blood vessels and cell elements of rat brain caused by photochemically induced focal ischaemia. | the lectin technique was applied to the histochemical studies of glycoconjugates comprising membranes of capillary endothelial cells and other cellular elements of the cns. in the studies we used the following lectins: concanavalin a (con a), bandeirea simplicifolia agglutinin (bs-i), helix pomatia agglutinin (hpa), ricinus communis agglutinin (rca-120), wheat germ agglutinin (wga). we noticed changes in the localisation of the glycoconjugates, which are the result of cerebral ischaemia caused b ... | 2002 | 12572776 |
| identification, purification and analysis of a 55 kda lectin binding glycoprotein present in breast cancer tissue. | the helix pomatia agglutinin (hpa)-binding glycoproteins from primary breast cancers and their metastases were compared with appropriate normal control tissues on western blots. from these studies a single glycoprotein of 55 kda was found to bind hpa in tumours but not in normal control tissues. the glycoprotein was identified by protein sequencing as being homologous to human immunoglobulin heavy chain variable region. subsequent immunostaining showed it to be immunoglobulin subclass a. iga1 wa ... | 1996 | 8894309 |
| the expulsion of echinostoma trivolvis from c3h mice: differences in glycoconjugates in mouse versus hamster small intestinal mucosa during infection. | mucosal glycoconjugates were examined in c3h mice and in hamster small intestines infected with echinostoma trivolvis and in uninfected rodents, using periodic-acid schiff (pas) and high-iron diamine-alcian blue (hid-ab) staining and three different fluorescein-conjugated lectins: triticum vulgaris agglutinin (wga), helix pomatia agglutinin (hpa) and griffonia simplicifolia agglutinin (gsa-ii). lectin-labelling by electron microscopy was also undertaken with wga and hpa lectin-gold probes. hid-a ... | 1996 | 8960206 |
| hemocyte-mediated phagocytosis and melanization in the mosquito armigeres subalbatus following immune challenge by bacteria. | mosquitoes are important vectors of disease. these insects respond to invading organisms with strong cellular and humoral immune responses that share many similarities with vertebrate immune systems. the strength and specificity of these responses are directly correlated to a mosquito's ability to transmit disease. in the current study, we characterized the hemocytes (blood cells) of armigeres subalbatus by morphology (ultrastructure), lectin binding, enzyme activity, immunocytochemistry, and fu ... | 2003 | 12838409 |
| [biological assay of preparations of helix pomatia l..]. | | 1954 | 13189202 |
| lectins in drug delivery: a study of the acute local irritancy of the lectins from solanum tuberosum and helix pomatia. | lectins are proteins or glycoproteins of non-immune origin capable of binding to one or more specific sugar residues. the potential for using lectins as a means of 'anchoring' a drug delivery system to the mucosal surfaces of the eye has been investigated in previous work, with the lectins from solanum tuberosum and helix pomatia showing particular promise. in this study the acute local dermal irritancy of these lectins, in terms of their potential to cause inflammation and tissue necrosis, was ... | 1999 | 10494002 |
| the comparative ability of beta-glucuronidase preparations (liver, escherichia coli, helix pomatia, and patella vulgata) to hydrolyze certain steroid glucosiduronic acids. | | 1961 | 13782588 |
| spheroplasts of the yeast candida utilis. | svihla, g. (argonne national laboratory, argonne, ill.), f. schlenk, and j. l. dainko. spheroplasts of the yeast candida utilis. j. bacteriol. 82:808-814. 1961.-the formation and properties of spheroplasts of the yeast candida utilis, produced by digestion of the cell walls with snail gut juice (helix pomatia) in isotonic medium, were studied by phase, interference, and ultraviolet microscopy, and tracer techniques. cells cultivated in the presence of l-methionine, s-methyl-l-methionine, and l-h ... | 1961 | 13918716 |
| a comparison of hydrolase and lytic activities of the digestive juices of the giant african snail, achatina fulica, and helix pomatia. | | 1963 | 13981006 |
| some cytological and pathogenic properties of spheroplasts of candida albicans. | kobayashi, george s. (tulane university, new orleans, la.), lorraine friedman, and judith f. kofroth. some cytological and pathogenic properties of spheroplasts of candida albicans. j. bacteriol. 88:795-801. 1964.-spheroplasts of candida albicans were prepared by use of an enzymatic mixture from the digestive tract of the snail helix pomatia. untreated cells exhibited well-defined cell walls, whereas such structures were absent from spheroplasts. the intravenous inoculation of either spheroplast ... | 1964 | 14208520 |
| [studies on the acetylcholinesterase in the blood of helix pomatia. 3. a combined optical test for determining the enzyme activity]. | | 1964 | 14236172 |
| beta-galactosidase of helix pomatia. | | 1964 | 14236292 |
| urinary excretion of c-20-reduction products of corticosterone. | 1. a 200 mg. portion of corticosterone was ingested by a healthy man and the urine collected. part of the urine was treated with the gastric juice of helix pomatia and extracted with ethyl acetate, and the extract fractionated with girard t. paper-chromatographic separation of the non-ketonic fraction in the bush (1952) system b(5) revealed the presence of two unknown polar components. 2. the unknown compounds did not possess a reducing (blue tetrazolium) or a reducible (potassium borohydride) g ... | 1965 | 14342242 |
| expression of cpgp40/15 in toxoplasma gondii: a surrogate system for the study of cryptosporidium glycoprotein antigens. | cryptosporidium parvum is a waterborne enteric coccidian that causes diarrheal disease in a wide range of hosts. development of successful therapies is hampered by the inability to culture the parasite and the lack of a transfection system for genetic manipulation. the glycoprotein products of the cpgp40/15 gene, gp40 and gp15, are involved in c. parvum sporozoite attachment to and invasion of host cells and, as such, may be good targets for anticryptosporidial therapies. however, the function o ... | 2003 | 14500524 |
| effect of sugars on the association between cowpea vicilin (7s storage proteins) and fungal cells. | vicilins (7s storage proteins) found in various legume seeds have been previously shown to interfere with the germination of spores or conidia of phytopathogenic fungi and inhibit yeast growth and glucose stimulated acidification of the medium by yeast cells. in the present work vicilins from cowpea (vigna unguiculata) seeds were added to the growth medium of saccharomyces cerevisiae cells and fusarium oxysporum conidia. helix pomatia lectin, wheat germ agglutinin and ulex europaeus lectin were ... | 2003 | 14510235 |
| optimized glucuronide hydrolysis for the detection of psilocin in human urine samples. | in order to develop a sensitive and reliable analytical method for psilocin (pc) in urine samples, the hydrolysis conditions including the acid, alkaline and enzymatic hydrolyses have been investigated by monitoring not only pc but also psilocin glucuronide (pcg) by liquid chromatography tandem mass spectrometry (lc-ms-ms); pcg was initially identified in a "magic mushroom (mm)" user's urine by liquid chromatography mass spectrometry (lc-ms) and lc-ms-ms. the proposed conditions optimized for th ... | 2003 | 14581081 |
| the parasitic trematode fasciola hepatica exhibits mammalian-type glycolipids as well as gal(beta1-6)gal-terminating glycolipids that account for cestode serological cross-reactivity. | neutral glycosphingolipids from sheep-derived fasciola hepatica liver flukes were isolated and characterized both structurally and serologically. after hplc fractionation, glycolipids were analyzed by linkage analysis, enzymatic cleavage, and maldi-tof as well as electrospray ionization mass spectrometry. obtained results revealed the presence of two types of neutral glycolipids. the first group represented mammalian-type species comprising globo- and isoglobotriaosylceramides (gal(alpha1-4)gal( ... | 2004 | 14638629 |
| metabolism of the soy isoflavones daidzein, genistein and glycitein in human subjects. identification of new metabolites having an intact isoflavonoid skeleton. | epidemiological studies have associated high soy intake with a lowered risk for certain hormone-dependent diseases. soy and soy foods are rich sources of isoflavones, which have been shown to possess several biological activities. in this study, the metabolism of soy isoflavones daidzein, genistein and glycitein was investigated in human subjects. the aim was to find and identify urinary phase i metabolites of isoflavones, which have an intact isoflavonoid skeleton, and which might possess some ... | 2003 | 14698210 |
| determination of urinary 18 beta-glycyrrhetinic acid by gas chromatography and its clinical application in man. | a sensitive and quantitative gas chromatographic assay for the determination of 18 beta-glycyrrhetinic acid (18 beta-ga), the main metabolite of glycyrrhizin after oral licorice consumption in human urine, has been developed and validated. for the extraction of 18 beta-ga from urine two sep-pak c18 extractions, hydrolysis with helix pomatia and three liquid-liquid extractions were performed, using 18 alpha-glycyrrhetinic acid (18 alpha-ga) as internal standard. both 18 beta-ga and internal stand ... | 1999 | 10510787 |
| the involvement of helix pomatia lectin (hpa) binding n-acetylgalactosamine glycans in cancer progression. | the lectin from helix pomatia, the roman snail (hpa), recognises terminal alpha n-acetylgalactosamine residues. a large number of lectin histochemical studies have demonstrated that expression of hpa-binding glycoproteins by cancer cells to be a marker of metastatic competence and poor prognosis in a range of common human adenocarcinomas, including those of breast, stomach, ovary, oesophagus, colorectum, thyroid and prostate. around 80% of metastases arising from primary breast cancer are predic ... | 2000 | 10668205 |
| monoclonal antibodies against the tn-specific isolectin b4 from vicia villosa seeds: characterization of the epitope of the blocking antibody vv34. | vicia villosa isolectin b4 (vvlb4) recognizes the tn antigen (galnac-o-ser/thr) exposed in certain human carcinomas. we have produced anti-vvlb4 monoclonal antibodies (mabs), and their lectin recognition selectivity was assessed by elisa and western blot against the purified gal/galnac-specific lectins from vicia villosa, salvia sclarea, helix pomatia, arachis hypogaea, glycine max, and dolichos biflorus. the antibodies were also tested for their ability to block the binding of vvlb4 to the tn a ... | 2004 | 15000847 |
| structural and functional characteristics of lung macro- and microvascular endothelial cell phenotypes. | lung macro- and microvascular endothelial cells exhibit unique functional attributes, including signal transduction and barrier properties. we therefore sought to identify structural and functional features of endothelial cells that discriminate their phenotypes in the fully differentiated lung. rat lung macro- (paec) and microvascular (pmvec) endothelial cells each exhibited expression of typical markers. screening for reactivity with nine different lectins revealed that glycine max and griffon ... | 2004 | 15020205 |
| effects of valproate derivatives i. antiepileptic efficacy of amides, structural analogs and esters. | derivatives of the antiepileptic drug valproate (vpa, 2-propylpentanoic acid) have been synthesized and tested in order to improve the intracellular availability of vpa. the buccal ganglia of helix pomatia were used as a test nervous system and antiepileptic efficacies were reconfirmed using rat cortex in vivo. epileptiform activities consisted of typical paroxysmal depolarization shifts (pds) which appeared in the identified neuron b3 with application of pentylenetetrazol. epileptiform activiti ... | 2000 | 10670421 |
| lectin ligands on human dendritic cells and identification of a peanut agglutinin positive subset in blood. | as only a few cell surface markers for dendritic cells (dc) have been identified to date, this study examined the expression of ligands for lectin on different human dc populations. the ability of concanavalin a (con a), wheat germ agglutinin (wga), peanut agglutinin (pna), and helix pomatia (hpa) to bind to cell lines and pbmc and dc populations was analyzed by flow cytometry and specificity of binding confirmed using inhibitory and noninhibitory sugars. the cell lines showed non-lineage-restri ... | 2000 | 10716881 |
| ability of blood group a-active glycosphingolipids to act as escherichia coli heat-labile enterotoxin receptors in ht-29 cells. | we examined the ability of blood group a-active glycoconjugates to act as receptors for escherichia coli heat-labile type i enterotoxin (lt-i) in ht-29 cells. these cells contained ~4 times more specific binding sites for lt-i than for cholera toxin (ct). binding of lt-i could not be blocked by the b subunit of ct (ct-b), indicating the existence of lt-i receptors in addition to the glycosphingolipid gm1. lt-i was able to increase levels of cyclic adenosine monophosphate (amp), even in the prese ... | 2004 | 15116290 |
| herpes simplex virus type-2 specific glycoprotein g-2 immunomagnetically captured from hep-2 infected tissue culture extracts. | monoclonal antibody h1206 anti-hsv-2 gg-2 bound to tosylactivated paramagnetic dynabeads (dynal) has been used to isolate hsv-2 type-specific gg-2 from solubilized hep-2 hsv-2 infected cell extracts. the immunomagnetically captured type-specific glycoprotein reacted strongly with monoclonal antibody h1206 and demonstrated a single band with apparent molecular weight of 100000 (100 kda) and a doublet band with an apparent molecular weight of 60000-64000 (60-64 kda). we observed the same exact ban ... | 2004 | 15158593 |
| effects of lectins on calcification by vesicles isolated from aortas of cholesterol-fed rabbits. | advanced vascular calcification in atherosclerosis weakens arterial walls, thereby imposing a serious rupturing effect. however, the mechanism of dystrophic calcification remains unknown. although accumulating morphological and biochemical evidence reveals a role for calcifiable vesicles in plaque calcification, the mechanism of vesicle-mediated calcification has not been fully explored. to study whether vesicles' membrane components, such as carbohydrates, may have a role in vesicle-mediated ca ... | 2000 | 10727613 |
| sperm binding to oviductal epithelial cells in the rat: role of sialic acid residues on the epithelial surface and sialic acid-binding sites on the sperm surface. | the aim of this study was to assess the participation of carbohydrate residues in the adhesion of spermatozoa to the oviductal epithelium in the rat. we first examined, by lectin labeling, the distribution of glycoconjugates in rat oviducts obtained under various hormonal environments. several classes of glycoconjugates were abundant in the epithelium, and the expression of some of these molecules varied differentially in ampulla and isthmus, along the estrous cycle and with estradiol and proges ... | 2004 | 15201197 |
| epileptogenicity and epileptic activity: mechanisms in an invertebrate model nervous system. | epileptic seizures are based on paroxysmal depolarization shifts (pds) which are synchronized in many neurons. mechanisms underlying pds and seizures are still not understood. the present review is based on studies using the buccal ganglia of the snail helix pomatia as a model nervous system. essential mechanisms of epileptic activity in nervous systems are thought to be identical in whatever part of the human or animal nervous system epileptic activity appears. from studies using the buccal gan ... | 2004 | 15216913 |
| time-dependent actions of aluminates on membrane and action potentials of snail neurons. | aluminum (al) is one of the elements, which is frequently subjected to experiments, however, the neurological observations with it are rather conflicting. the cause of this controversiality is not known but relates to some human disorders such as alzheimer's disease and others as well. we studied the time-dependent actions of alcl3 base solutions on resting membrane potential (em), input resistance (rin) and spike shape in giant neurons of the snail helix pomatia l. at ph 7.7 and room temperatur ... | 2000 | 11034155 |
| enzymatic hydrolysis of conjugated steroid metabolites: search for optimum conditions using response surface methodology. | the optimum conditions for hydrolysing conjugated metabolites of steroid hormones in bovine urine were performed with helix pomatia juice, beta-glucuronidase from bovine liver and preparations of limpets and abalone entrails using response surface methodology. the experimental design and empirical modelling used allowed us to assess the main effects of factors (time, temperature, ph and enzyme quantity) and to predict the optimum conditions for each enzyme preparation. confirmatory experiments w ... | 2000 | 11219062 |
| helix pomatia a hemagglutinin, a surface marker for bovine t-lymphocytes. | bovine peripheral blood lymphocytes (pbl) were isolated from blood collected from 6 cattle. after treatment with neuraminidase, 40 or 60% of the cells were shown to combine with helix pomatia a hemagglutinin (hp) depending whether a direct or indirect fluorescence technique was used. about 20% of the cells were ig-bearing. with double staining fluorescence technique, it was shown that cells attaching to hp were not ig-bearing and the reverse. with the aid of hp, covalently bound to sepharose, ig ... | 1979 | 15612267 |
| a study of porcine lymphocyte populations. i. separation of porcine lymphocyte subpopulations. | several methods for separating porcine lymphocyte populations were studied. lymphocyte populations enriched in t cells were obtained by filtration on nylon fiber columns. lymphocyte populations enriched in b cells were obtained by e rosette sedimentation. results obtained with anti-immunoglobulin columns were less satisfactory. attempts to fractionate porcine lymphoid cells with the help of helix pomatia lectin remained unsuccessful. | 1980 | 15615056 |
| detection of synthetic glucocorticoid residues in cattle tissue and hair samples after a single dose administration using lc-ms/ms. | a sensitive and specific liquid chromatography-tandem mass spectrometry (lc-ms/ms) assay for the detection of several synthetic glucocorticoids in kidney, muscle and hair samples of cattle after a single intramuscular injection is described. after a dichloromethane wash of the hair samples, analytes were released from the hair matrix by enzymatic digestion. muscle samples were also digested enzymatically using proteinase, while kidney samples were deconjugated by helix pomatia juice. these preli ... | 2005 | 15686988 |
| supercooling ability in two populations of the land snail helix pomatia (gastropoda: helicidae) and ice-nucleating activity of gut bacteria. | the land snail helix pomatia (gastropoda: helicidae) is widely distributed in northern and central europe where it may experience subzero temperatures during winter months. its supercooling ability was studied in two populations of h. pomatia. one population originated from southern sweden (gotaland) and the other from central france (auvergne). in the experimental design, they were acclimated, over 2 weeks, to artificial winter conditions (hibernation, t=5 degrees c). the swedish snails showed ... | 2004 | 15710369 |
| cancer metastasis: characterization and identification of the behavior of metastatic tumor cells and the cell adhesion molecules, including carbohydrates. | this review focuses on the behavior of metastatic tumor cells and their specific adhesion molecules. much of this review is based on the results from our researches over many years. electron microscopic investigations of metastatic processes have demonstrated that desmosomes, tight junctions, or cell fusion-like structures are formed between tumor cells and other cells such as endothelial, mesothelial, hepatic, and nerve cells. these findings suggest that metastatic tumor cells acquire specific ... | 2005 | 15720223 |
| enrichment of cheeses manufactured from cow's and sheep's milk blends with sheep-like species-related alkylphenols. | enhancement of concentrations of species-related sheep-like alkylphenols, p- and m-cresols and 3- and 4-ethylphenols, in experimental manchego-type cheeses manufactured from cow's and sheep's milk blends (80:20) by using arylsulfatases was investigated. a food-grade arylsulfatase from aspergillus oryzae (atcc 20719) was produced using a stimulatory medium, and crude dried cells were used as the enzyme source. exogenous arylsulfatases from helix pomatia and a. oryzae were added to cheese curd, an ... | 2005 | 15740062 |
| the tidemark of the chondro-osseous junction of the normal human knee joint. | the chondro-osseous junction includes the junction between calcified and non-calcified cartilage matrices often referred to as the tidemark. a detailed knowledge of the structure, function and pathophysiology of the chondro-osseous junction is essential for an understanding both of the normal elongation of bones and of the pathogenesis of osteoarthrosis. in this study the molecular anatomy of the tidemark was studied using histochemical techniques, including lectin histochemistry, on blocks of n ... | 2005 | 15900412 |
| optimization of conditions for the enzymatic hydrolysis of phytoestrogen conjugates in urine and plasma. | optimal ph, temperature, and concentration of enzyme conditions for the rate of hydrolysis of five isoflavone conjugates (daidzein, o-desmethylangolensin, equol, genistein, and glycitein) and two lignans (enterodiol and enterolactone) from two biological matrices (urine and plasma) were studied using beta-glucuronidase from helix pomatia. in addition, the use of mixtures of beta-glucuronidase and sulfatase enzymes from different sources was investigated to find enzyme preparations that contained ... | 2005 | 15907867 |
| gas chromatograph-mass spectrometric method for the determination of carvedilol and its metabolites in human urine. | a sensitive and efficient method was developed for the determination of carvedilol and its metabolites in human urine by gas chromatography-mass spectrometry (gc-ms). urine samples were hydrolyzed with beta-glucuronidase/arylsulfatase (from helix pomatia) and the target compounds were extracted with liquid-liquid extraction. the extracts were completely derivatized with mstfa and mbtfa and analyzed by gc-ms using an ultra-2 column. the linearity of the assay ranges were 0.75-75 ngml(-1) for carv ... | 2005 | 15996536 |
| the lectin helix pomatia agglutinin as a marker of metastases--clinical and experimental studies. | the prognosis for the majority of patients suffering from a solid neoplasm remains bleak, due to the fact that most cancers have already formed metastases at distant sites. any progress in cancer therapy will, therefore, depend on the understanding of the metastatic cascade and therapies derived from this understanding. the development of clinically relevant models of metastases, using lectin-defined human cancer cell lines and severe combined immunodeficient (scid) mice, is described. in conjun ... | 2005 | 16033108 |
| hydrolysis of conjugated metabolites of buprenorphine ii. the quantitative enzymatic hydrolysis of norbuprenorphine-3-beta-d-glucuronide in human urine. | in gas chromatographic-mass spectroscopic analysis of buprenorphine metabolites, the urine specimen must be first hydrolyzed to release buprenorphine and norbuprenorphine from their glucuronide conjugates. for evaluation of existing hydrolysis methods and to find out the optimal hydrolysis conditions, buprenorphine-3-beta-d-glucuronide (b3g) and norbuprenorphine-3-beta-d-glucuronide (nb3g) were synthesized. in a previous communication we reported on the optimum conditions for hydrolysis of b3g. ... | 2005 | 16168181 |
| membrane mechanisms of antiarrhythmic effect of quaternidine. | complex electrophysiological study of the effects of quaternidine carried out on intact hearts from cats, myocardial fragments from rats, and single ionic channels of large edible snail showed that quaternidine demonstrates properties of class 1b antiarrhythmic drug according to vaughan-williams nomenclature. this agent did not suppress nomotopic pacemaker automaticity, did not change conduction in ventricles, atria, and atrioventricular junction in hearts with preserved sinus rhythm, did not pr ... | 2005 | 16224582 |
| evidence for the cd(2+) activation of the aryl sulfatase from helix pomatia. | often used to remove sulfate groups from carbohydrates, the regulatory properties of the aryl sulfatase from helix pomatia remain little characterized. as many hydrolytic enzymes utilize exogenous metal ions in catalysis, the effect of various divalent metal ions on the sulfatase was investigated. evidence for metal ion activation was collected, with cd(2+) being notable for effective activation. the enzyme was inhibited by cu(2+). the response of other common hydrolases to divalent metal ions w ... | 2005 | 16333754 |
| purification of the crude solution from helix pomatia for use as beta-glucuronidase and aryl sulfatase in phytoestrogen assays. | phytoestrogens occur in a variety of foods and are thought to offer a protective effect against a number of complex diseases. due to the diversity of phytoestrogen conjugates formed in the human body, most assays include an enzymatic hydrolysis step prior to analysis. beta-glucuronidase from helix pomatia, which also contains sulfatase activity, is popular for this task but contains appreciable levels of some phytoestrogens and related compounds, which could affect accurate quantification at low ... | 2006 | 16388994 |
| isolation and characterization of metabolically competent pulmonary epithelial cells from pig lung tissue. | administration of drugs by inhalation opens new possibilities for entry into the systemic circulation and cultures of porcine pulmonary epithelial cells (pecs) may prove to be valuable in the prediction of pulmonary metabolism of drugs in humans. this paper, therefore, reports a method for the routine isolation and cultivation of pecs from slaughterhouse animals. on average 1.5x10(6) cells g-1 tissue were isolated by discontinuous density-gradient centrifugation. cells were subsequently cultivat ... | 2005 | 16393853 |
| in vivo xenograft models of breast cancer metastasis. | if breast cancer patients are not cured, it is largely because of the fact that the cancer has spread beyond its primary site--the breast--to distant sites, such as, e.g., bone marrow, lung, brain, and/or liver. these secondary tumors are called metastases, and the underlying mechanisms leading to these secondary tumor deposits are complex and still ill understood. in this chapter, we report on how to develop clinically relevant human breast cancer cell line xenografts in severe combined immunod ... | 2006 | 16491620 |
| helix pomatia agglutinin lectin-binding oligosaccharides of aggressive breast cancer. | predicting long-term outcome after breast-cancer diagnosis remains problematic, particularly for patients with clinically small, axillary lymph node- negative tumours. evidence suggests that the lectin helix pomatia agglutinin (hpa) identifies oligosaccharides associated with poor-prognosis cancer. our aim was to identify oligosaccharides that bind hpa in aggressive breast cancers. breast-cancer cell lines (mcf-7, bt-549 and bt-20) and a cell line from human milk (hbl-100), which showed a range ... | 2001 | 11241316 |
| alternative methodology for the analysis of progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle. | research has shown that traditional solvent extraction procedures used for the analysis of endogenous steroids often give inconsistent recoveries and results. however, a single-laboratory validation of a liquid chromatography/tandem mass specrometry method using 2 product ions per transition for progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle showed accuracy and precision to within 23% at concentrations ranging from 0.5 to 2.0 microg/kg. homogenized samples were ... | 2006 | 16640309 |
| n-acetylgalactosamine, n-acetylglucosamine and sialic acid expression in primary breast cancers. | binding of the lectin from helix pomatia (hpa), which recognises n-acetylgalactosamine and n-acetylglucosamine glycans, is a predictor of metastasis and poor prognosis in a number of human adenocarcinomas, including breast cancer. the glycoproteins to which it binds in these tumours have been only partially characterised, and the mechanisms underlying their biosynthesis remain unknown. in this study, 111 primary breast cancers were assessed for binding of hpa and labelling characteristics were c ... | 2001 | 11252626 |
| the use of lectins to characterize and separate living canine spermatozoa: a preliminary study. | this study was designed as a preliminary attempt to develop a methodology for relating the glucidic structure of the sperm membrane to sperm morphology. differences in plasma membrane glycoconjugates between motile and nonmotile spermatozoa were studied by using 7 lectins. fresh spermatozoa from 3 dogs were analyzed by fluorescein isothiocyanate (fitc) labeled lectins. the binding of lectins to the sperm membrane and the capability of the lectins to agglutinate spermatozoa were estimated semi-qu ... | 1994 | 16727393 |
| [comparative analysis of map/erk-kinase activation in the cns of animals with different capability for learning]. | with the western-blot analysis, we studied the activation of map/erk protein kinases (regulating genes expression by the phosphorylation of transcription factors (tf) creb and elk-1) in native helix pomatia and in animals with disturbed abilities to form long-term conditioned avoidance reflexes. different stages of this reflex formation were shown to be characterized by different levels of map/erk kinases activation, the latter being different in cns's regions (visceral complex of ganglia, cereb ... | 2006 | 16869282 |
| functional interaction of escherichia coli heat-labile enterotoxin with blood group a-active glycoconjugates from differentiated ht29 cells. | human colon adenocarcinoma cells (ht29-atcc) and the clone ht29-5f7 were cultured under conditions that differentiate cells to a polarized intestinal phenotype. differentiated cells showed the presence of junctional complexes and intercellular lumina bordered by microvilli. intestinal brush border hydrolase activities (sucrase, aminopeptidase n, lactase and maltase) were detected mainly in differentiated ht29-atcc cells compared with the differentiated clone, ht29-5f7. the presence of non-gm1 re ... | 2006 | 16884490 |
| an accurate and reproducible method for the quantitative analysis of isoflavones and their metabolites in rat plasma using liquid chromatography/mass spectrometry combined with photodiode array detection. | to study the safety and potential health benefits of soy isoflavones, a rapid and simple method based on liquid chromatography combined with mass spectrometry (lc/ms) and photodiode array detector (pda) was developed for the determination of isoflavones in rat plasma. the analytes included daidzein, genistein, glycitein, equol, 4-ethyl phenol, and biochanin a over a concentration range of 1.0-4320.0 nm using 75 microl of rat plasma. rat plasma samples were hydrolyzed by adding an enzyme mixture ... | 2006 | 16915859 |
| comparative study of blood group-recognizing lectins toward abo blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides. | binding specificities of abo blood group-recognizing lectins toward blood group antigens on neoglycoproteins, glycoproteins and complex-type oligosaccharides were studied by lectin-blotting analysis, enzyme linked immunosorbent assay and lectin-conjugated agarose column chromatography. human serum albumin conjugated with a- and b-trisaccharides was clearly recognized by helix pomatia (hpa), phaseolus lunatus, dolichos biflorus agglutinins, and griffonia simplicifolia i agglutinin b(4), respectiv ... | 2001 | 11342253 |
| assessing the instability of the isoelectric focusing patterns of erythropoietin in urine. | ief can be used to differentiate human urinary erythropoietin (uepo), recombinant human erythropoietin or epoetin (repo) and darbepoetin (novel erythropoiesis stimulating protein (nesp)). this is the basis of the method currently used to detect misuse of repo and nesp by elite athletes. recently, an unknown activity has been attributed to some urine samples (denominated 'unstable' urine by the world anti-doping agency; wada). this activity has shown to give rise to artefactual profiles for both ... | 2006 | 17054082 |