| comparative study of amino acid contents and ldh isozyme by electrophoresis and slab-page in adult worms of ascaris lumbricoides, ascaris suum and toxocara canis. | | 1987 | 3127150 |
| role of cyclooxygenase products of arachidonic acid metabolism in ascaris antigen-induced bronchoconstriction in sensitized dogs. | the pharmacologic modulation of ascaris allergen-induced bronchoconstriction was investigated in beagle dogs sensitized by experimental infection with ascaris suum ova. ascaris-induced increases in total lung resistance (rl) and falls in dynamic lung compliance (cdyn) were unaffected by pretreatment with an antihistamine (pyrilamine) given alone but were significantly attenuated (p less than .05) by the cyclooxygenase enzyme inhibitor, indomethacin. the combination of pyrilamine and indomethacin ... | 1988 | 3130478 |
| modification of bronchial blood flow during allergic airway responses. | ascaris suum antigen effects on mean airflow resistance (rl) and bronchial arterial blood flow (qbr) were studied in allergic anesthetized sheep with documented airway responses. qbr was measured with electromagnetic flow probes, and supplemental o2 prevented antigen-induced hypoxemia. aerosol challenge with this specific antigen increased rl and qbr significantly. cromolyn sodium aerosol pretreatment prevented antigen-induced increases in rl but not in qbr. intravenous cromolyn, however, preven ... | 1988 | 3136132 |
| late-phase reaction in topically induced ocular anaphylaxis in the rat. | a cellular late-phase reaction is described in a rat model of topically induced ocular anaphylaxis. rats were immunized with dinitrophenylated ascaris suum extract and alum and were tested for active cutaneous anaphylaxis on day 13. rats with a strong skin test response were selected for ocular challenge with di-dnp-lysine. macroscopic observation and histologic evaluation were performed at 1, 6, and 24 h. in rats showing a moderate macroscopic ocular response at 1 h, mast cell degranulation was ... | 1988 | 3136973 |
| attenuation of rat conjunctival response by repeated hapten applications. | attenuation of the rat conjunctival response by repeated topical challenge with dinitrophenyl (dnp) hapten was demonstrated in our study. adult rats were immunized by intraperitoneal injections of dinitrophenylated ascaris suum extract (dnp-asc) and alum. serum levels of anti-dnp homocytotropic antibody were determined by passive cutaneous anaphylaxis in rats prepared with antibody 48 hours earlier. in other animals, topical challenge was performed by applying n,n'-di-2,4-dnp-l-lysine (di-dnp-ly ... | 1988 | 3141114 |
| chalone-like inhibition of dna synthesis in helminths. | chalone-like inhibitory activity was found in extracts from tissues of taenia crassiceps cysticercus and tissues of adult ascaris suum. the inhibitory effects were partially species nonspecific: the extracts from t. crassiceps larvae inhibit both t. crassiceps and mesocestoides corti larvae dna synthesis. extracts from a. suum, however, inhibit only t. crassiceps. besides them, the inhibitory factor from mouse brain inhibits both t. crassiceps and m. corti dna synthesis. when the inhibitory fact ... | 1985 | 3159635 |
| efficacy of flubendazole against gastrointestinal and lung nematodes in pigs. | three trials were carried out on landrace pigs of various ages to assess the anthelmintic efficacy of flubendazole. the pigs were either artificially infected with metastrongylus apri or naturally or artificially infected with the gastrointestinal nematodes ascaris suum, oesophagostomum dentatum or hyostrongylus rubidus. for mass medication of young pigs and fatteners a dose regimen of 30 ppm flubendazole in the feed for 10 consecutive days was 100 per cent effective against the four nematode sp ... | 1988 | 3195017 |
| phosphorylation and inactivation of the pyruvate dehydrogenase from the anaerobic parasitic nematode, ascaris suum. stoichiometry and amino acid sequence around the phosphorylation sites. | tryptic digestion of the fully phosphorylated ascaris suum pyruvate dehydrogenase complex yielded a single tetradecapeptide containing 2 phosphorylated serine residues. its amino acid sequence was tyr-ser-gly-his-ser(p)-met-ser-asp-pro-gly-thr-ser(p)-tyr-arg and was very similar to one of the tryptic phosphopeptides isolated from mammalian and yeast pyruvate dehydrogenases. at partial phosphorylation, three peptides were isolated which corresponded to the monophosphorylated (sites 1 and 2) and d ... | 1988 | 3198613 |
| superoxide dismutase from ascaris suum. | three superoxide dismutases (sod) (ec 1.15.1.1) were detected in homogenates of ascaris suum. each of the three forms of sod was purified by a sequence of multiple differential centrifugations, ammonium sulphate precipitation, ion-exchange chromatography and g-75 sephadex column chromatography. the three forms of sod were present in different cellular locations; one in the cytoplasmic fraction, sensitive to cyanide and hydrogen peroxide, and two in the mitochondrial fraction, one of which was cy ... | 1988 | 3200607 |
| cellular markers of inflammation in the airways of allergic sheep with and without allergen-induced late responses. | allergic sheep respond to inhaled ascaris suum antigen either with an acute bronchoconstriction alone (acute responders, ar) or both an acute and late bronchoconstriction (dual responders, dr). in this study, we determined if: (1) inflammatory cell composition of bronchoalveolar lavage (bal) obtained during the late response differs between dr and ar; (2) the difference in inflammatory cells is dependent on the prechallenge bal cell composition; and (3) drugs that block late airway responses als ... | 1988 | 3202508 |
| a leukotriene and thromboxane inhibitor (sch 37224) blocks antigen-induced immediate and late responses and airway hyperresponsiveness in allergic sheep. | peptide leukotrienes and thromboxane a2 are putative mediators of allergen-induced late responses and allergen-induced airway hyperresponsiveness (ahr), respectively. sch 37224 blocks antigen-induced leukotriene d4 and thromboxane b2 release in guinea pig lung fragments. it also inhibits leukotriene-mediated allergic guinea pig bronchospasm. sch 37224 was, therefore, tested in allergic sheep (n = 6) with documented immediate and late airway responses and ahr to inhaled ascaris suum antigen. for ... | 1988 | 3204511 |
| the induction of permeability in egg-shells of ascaris suum prior to hatching. | | 1988 | 3225120 |
| suppression of ige antibody production by ascaris suum extract: characterization of suppressive component(s). | partial characterization of the suppressive component(s) of a. suum extract that is (are) responsible for damping production of ige antibody to ovalbumin was performed by physical and chemical methods. digestion of the whole extract with trypsin and chymotrypsin completely abolished the suppressive activity. oxidation with sodium metaperiodate or heating at 56 degrees c, however, had no effect. these results indicate that the integrity of heat-stable protein(s) present in the crude extract is es ... | 1988 | 3228633 |
| [inhibitory effects of extracts of ascaris suum on human blood coagulation]. | | 1988 | 3253027 |
| isotope partitioning for nad-malic enzyme from ascaris suum confirms a steady-state random kinetic mechanism. | isotope partitioning studies beginning with e.[14c]nad, e.[14c]malate, e.[14c]nad.mg2+, and e.mg.[14c]malate suggest a steady-state random mechanism for the nad-malic enzyme. isotope trapping beginning with e.[14c]nad and with varying concentrations of mg2+ and malate in the chase solution indicates that mg2+ is added in rapid equilibrium and must be added prior to malate for productive ternary complex formation. equal percentage trapping from e.[14c]nad.mg and e.mg.[14c]malate indicates the mec ... | 1988 | 3280016 |
| proteinases released by the parasitic larval stages of ascaris suum, and their inhibition by antibody. | the tissue-invasive infective and lung-stage larvae of the nematode ascaris suum were found to release proteinases during culture in vitro. this activity contained multiple proteolytic enzyme activities, as defined by ph optima, substrate specificities, and inhibitor profiles. chymotryptic, tryptic collagenolytic and elastolytic activities were produced by both developmental stages, with major ph optima at ph 6 and 9, and there were indications of unusual interactions between the enzymes. the se ... | 1988 | 3290677 |
| effects of infection with enterobacteriaceae enteropathogens on subsequent infection with ascaris suum in the laboratory mouse. | prior to infection with ascaris suum, icr strain mice were inoculated with salmonella typhisuis intraperitoneally or via gastric gavage. similarly, salmonella cholerae-suis var. kunzendorf, salmonella typhimurium and enterotoxigenic escherichia coli were administered to mice via gastric gavage 2 weeks prior to a. suum inoculation. previous inoculation with s. typhisuis, via the intraperitoneal or gastric gavage routes and s. cholerae-suis var. kunzendorf decreased recovery of ascarid larvae from ... | 1987 | 3296422 |
| immunochemical characterization of the pyruvate dehydrogenase complex in adult ascaris suum and its developing larvae. | polyclonal antibody was prepared against the pyruvate dehydrogenase complex purified from adult ascaris suum body wall muscle. the antibody reacted with the e2, x, alpha e1 and beta e1 subunits of the complex in immunoblots of mitochondrial supernatant fractions and homogenates of adult muscle. in addition, the same subunits were observed in immunoblots of homogenates of l3 and l4 ascarid larvae, suggesting that a similar enzyme complex was present in all developmental stages despite their marke ... | 1987 | 3299084 |
| resistance of nematode secretory products to cleavage by mast cell proteinases. | mast cell proteinases are known to be released in response to helminth infection, and are, in particular, characteristic of the immune rejection of intestinal nematode parasites. in intestinal mucosal tissue the relevant enzyme is rat mast cell proteinase ii (rmcp ii) and that of other tissues, including the lung, is rat mast cell proteinase i (rmcp i). the function of these enzymes is unknown, and we have examined the possibility that they directly attack the parasites. this was done by examini ... | 1987 | 3306371 |
| toxocara canis: monoclonal antibodies to larval excretory-secretory antigens that bind with genus and species specificity to the cuticular surface of infective larvae. | when maintained in culture, the infective-stage larvae of toxocara canis produce a group of excretory-secretory antigens. monoclonal antibodies to these antigens have been produced and partially characterized. hybridomas were made using spleens from mice that had been given 250 embryonated eggs of t. canis followed by immunization with excretory-secretory antigens. monoclonal antibodies were first screened against excretory-secretory antigens using an indirect enzyme-linked immunosorbent assay. ... | 1987 | 3315735 |
| pulmonary bacterial deposition and clearance during ascarid larval migration in weanling pigs. | pulmonary deposition and clearance of bacteria were measured in weanling pigs, half of which had been inoculated at age 31 days with larvated ascaris suum ova. seven days later, when breathing signs of larval migration were pronounced, all pigs were exposed to aerosolized escherichia coli (strain b). then, either immediately after aerosol exposure (for deposition assessment) or immediately after a 120 minute period in filtered air (for clearance), bacteria in the pigs' lungs were counted. ascari ... | 1987 | 3330966 |
| ascaris suum antigens incorporated into liposomes used to stimulate protection to migrating larvae. | four- to 8-week-old spf pigs were immunized, using antigens of ascaris suum incorporated into liposomes, via intestinal cannula or orally. avridine was also incorporated in the liposomes in one experiment and interleukin-2 (il-2) injected into pigs in another experiment. a priming dose of embryonate eggs (80-470 eggs/pig) were given in four of six experiments. compared to control animals, the greatest protection of pigs to migrating ascarid larvae from a challenge dose of 10,000 embryonated eggs ... | 1988 | 3347994 |
| ascaris suum and onchocerca volvulus: s-adenosylmethionine decarboxylase. | putrescine-dependent s-adenosylmethionine decarboxylase (ec 4.1.1.50) was demonstrated in ascaris suum and onchocerca volvulus; activation was found to be about fourfold by putrescine. mg2+ did not affect the enzyme activity. a. suum was taken as a model nematode and its s-adenosylmethionine decarboxylase was partially purified and characterized. the molecular weight was estimated to be 220,000. the apparent km-value for adenosylmethionine was determined to be 17 microm. methylglyoxal bis(guanyl ... | 1988 | 3350107 |
| superoxide dismutase activity in extracts of specimens of ascaris suum and several analogous tissues in both sexes. | 1. the activities of the enzymes superoxide dismutase (ec.1.15.1.1), and catalase (ec.1.11.1.6) in purified extracts of whole ascaris suum adult males and females, and also in several analogous tissues of each sex, were studied. 2. no catalase activity was found in any of the extracts. 3. considerable superoxide dismutase activity was detected in both sexes and the levels of this activity showed sexual differences in the values found in different tissue locations. 4. the sexual organs of both ma ... | 1988 | 3359758 |
| effects of topical pre-treatment with dexamethasone on the immediate and late phases of topically induced ocular anaphylaxis in the rat. | we tested the effects of pre-treatment with dexamethasone on topically induced ocular anaphylaxis in the rat. rats were immunized with dinitrophenylated ascaris suum extract and challenged with di-dnp-lysine. dexamethasone was administered topically once (24, 6, or 1 h before challenge) or three times (6, 4, and 2 h before challenge). a single pre-treatment given at 24 or 6 h had no significant effect. a single pre-treatment 1 h before challenge reduced the extent of edema assessed histologicall ... | 1988 | 3364167 |
| ascaris suum: development of intestinal immunity to infective second-stage larvae in swine. | the development of protective immunity to ascaris suum was examined in pigs naturally exposed to eggs on a contaminated dirt lot. pigs became almost totally immune to second-stage larvae migrating from the intestines because few larvae from a challenge inoculum could be found in the lungs, and liver white-spot lesions (an immunopathologic response to migrating larvae) were absent. blood from these pigs contained lymphocytes that responded blastogenically to larval antigens in vitro, while the se ... | 1988 | 3366215 |
| comparative analysis of larval excretory-secretory antigens of baylisascaris procyonis, toxocara canis and ascaris suum by western blotting and enzyme immunoassay. | | 1988 | 3366527 |
| [three recent cases of ascariasis in northern kyushu]. | ascariasis is considered to be one of the rare infectious diseases in japan, but recently it has been slightly increasing. this paper reports three ascariasis cases who seemed to be infected recently in the kitakyushu area, japan. case 1: a 59-year-old woman in kitakyushu city passed a round worm after continuous abdominal pain. the patient was discharged from the hospital because of no further abnormal intestinal symptoms and findings. case 2: an 85-year-old woman in nakama city, who suffered f ... | 1988 | 3368668 |
| eosinophilia in ascaris suum-reinfected mice. | the secondary response of eosinophilia has been studied in mice infected with a. suum. in mice infected orally with 1000 a. suum eggs, larvae disappeared from the body within two weeks after infection. the number of peripheral blood eosinophils decreased to the pre-infection level within eight weeks. a typical secondary response of igg antibody production to egg antigen was found after reinfection with 1000 eggs. the number of peripheral blood eosinophils increased more rapidly after reinfection ... | 1988 | 3372980 |
| tryptophan hydroxylase and aromatic l-amino acid decarboxylase activities in the tissues of adult ascaris suum. | | 1988 | 3397216 |
| the effect of an orally active leukotriene (lt) d4 antagonist wy-48, 252 on ltd4 and antigen-induced bronchoconstrictions in allergic sheep. | in this study we examined the effects of a new orally active leukotriene (lt) d4 receptor antagonist, wy-48,252 (1,1,1-trifluoro-n-[3-(2-quinolinylmethoxy)phenyl]methanesulfonamide), on ltd4-induced bronchoconstriction and antigen-induced early and late responses in allergic sheep. for all studies wy-48,252 10 mg/kg, was administered via intragastric tube 1 h prior to airway challenge. in seven sheep, airway challenge with ltd4 [delivered dose mean +/- se, 53 +/- 2 micrograms] resulted in an imm ... | 1988 | 3399681 |
| rapid increase in mast cell numbers in canine central and peripheral airways. | in preliminary studies of antigen-induced airway inflammation, we noted an apparent increase in peribronchiolar mast cell number. experiments were thus undertaken to investigate the nature of this migration of mast cells into the central and peripheral airway epithelium and to determine its time course. the tracheae and small airways of 10 anesthetized mongrel dogs were exposed via a bronchoscope to ascaris suum antigen (ag), fmet-leu-phe (fmlp), ovalbumin (ova), and isotonic saline (sal). in th ... | 1988 | 3403488 |
| developmental changes in cuticular proteins of ascaris suum. | 1. cuticles were isolated from developmental stages of the swine nematode ascaris suum by a combination of mechanical disruption and detergent treatment of larvae or by surgical removal of cuticle from adults. proteins from the isolated cuticles were solubilized with 2-mercaptoethanol (2me) and analyzed by sds-page. 2. 2me soluble, cuticular proteins from adults consisted of 5 to 6 bands with 80% of proteins in 2 bands with mol. wts of 106,000 and 93,000. cuticular proteins from the third and fo ... | 1988 | 3409662 |
| histopathological changes in organs of the miniature pigs gtt infected with invasive larvae of ascaris suum goeze, 1782. | | 1987 | 3436470 |
| purification and properties of an acyl coa transferase from ascaris suum muscle mitochondria. | an acyl coa transferase has been purified to electrophoretic homogeneity from the soluble compartment of ascaris suum muscle mitochondria. from sds-page, isoelectric focusing and molecular exclusion chromatography, homogeneity was confirmed and the enzyme appears to be composed of two similar or identical subunits of apparent mol. wts of 50,000 resulting in an apparent mol. wt of 100,000 for the holoenzyme. the apparent isoelectric point was 5.6 +/- 0.1 by both chromatofocusing columns and slab ... | 1986 | 3456879 |
| [histochemical evaluation of steroidogenesis in the adrenal glands of guinea pigs infected with ascaris suum l. larvae]. | | 1986 | 3458342 |
| bizarre trnas inferred from dna sequences of mitochondrial genomes of nematode worms. | the complete nucleotide sequence of the mitochondrial dna (mtdna) molecule of the parasitic nematode worm ascaris suum has been determined. this molecule lacks genes for trnas of the standard form. instead, 21 sequences are found that can be folded into structures that resemble trnas in which the t psi c arm and variable loop are missing and replaced with a single loop of between 4 and 12 nucleotides. considerations of various properties of these sequences, including the number, predicted antico ... | 1987 | 3469671 |
| separation and functions of two acyl coa transferases from ascaris lumbricoides mitochondria. | many invertebrates accumulate propionate, or products derived from propionate, as products of fermentation. evidence has been reported that the nematode, ascaris suum, the cestode, spirometra mansonoides, and the trematode, fasciola hepatica, accumulate propionate by means of an adenosine triphosphate (atp)-generating decarboxylation of succinate. to generate energy, an acyl coenzyme a (coa) transferase that would transfer coa to succinate is required as one component of the sequence of reaction ... | 1987 | 3475402 |
| mechanisms of antigen-induced reactions in skin and lung. | investigators have tried to develop various experimental preparations suitable to study the effects of antigen on skin and airways in order to understand the mechanisms involved in asthma and to develop new methods of diagnosis, prevention and treatment. we have gained considerable experience in studies of specific antigens in inbred dogs with increased levels of serum ige antibodies to aeroallergens. by using a new method of sensitization, combining an attenuated live virus with a specific and ... | 1986 | 3494283 |
| protonation mechanism and location of rate-determining steps for the ascaris suum nicotinamide adenine dinucleotide-malic enzyme reaction from isotope effects and ph studies. | the ph dependence of the kinetic parameters and the primary deuterium isotope effects with nicotinamide adenine dinucleotide (nad) and also thionicotinamide adenine dinucleotide (thio-nad) as the nucleotide substrates were determined in order to obtain information about the chemical mechanism and location of rate-determining steps for the ascaris suum nad-malic enzyme reaction. the maximum velocity with thio-nad as the nucleotide is ph-independent from ph 4.2 to 9.6, while with nad, v decreases ... | 1986 | 3513825 |
| demonstration of carboxyl and thiol protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum. | evidence has been presented showing two kinds of acidic protease activities in adult schistosoma mansoni, dirofilaria immitis, angiostrongylus cantonensis and ascaris suum. a haemoglobinolytic activity without adding any sh-containing compounds was maximal at ph 3.5, 2.5, 3.0 and 3.5 in s. mansoni, d. immitis, angiostrongylus cantonensis and ascaris suum respectively. the inhibitor studies demonstrated that this activity is ascribable to carboxyl protease(s). in the presence of dithiothreitol, a ... | 1986 | 3517135 |
| seminal vesicle growth induced by ascaris suum infection. | this study was designed to determine the effect of ascaris suum infection upon growth of mouse vesicular glands. mice were infected with varying dosages of a. suum eggs and killed after six weeks. results indicated a dose dependent increase in seminal vesicle weight, independent of total body weight. | 1986 | 3564336 |
| efficacy, safety, and residue evaluation of levamisole gel formulation in sows. | anthelmintic efficacy, safety, and residue studies were conducted in sows and gilts with a levamisole gel containing 11.5% levamisole hcl. in 12 sows and 12 gilts, 8 mg of levamisole hcl equivalent/kg of body weight orally was 100% (resinate) and 91.1% (gel) effective against 55-day-old ascaris suum and 100% (gel) and 96.1% (resinate) effective against oesophagostomum dentatum. in 20 sows given levamisole gel (8 mg of levamisole hcl/kg) orally just before breeding, 4 to 6 weeks after breeding, 4 ... | 1987 | 3592389 |
| modification of an arginine residue essential for the activity of nad-malic enzyme from ascaris suum. | purified nad-malic enzyme from ascaris suum is rapidly inactivated by the arginine reagent, 2,3-butanedione, and this inactivation is facilitated by 30 mm borate. determination of the inactivation rate as a function of butanedione concentration suggests a second-order process overall, which is first order in butanedione. a second-order rate constant of 0.6 m-1 s-1 at ph 9 is obtained for the butanedione reaction. the inactivation is reversed by removal of the excess reagent upon dialysis. the en ... | 1987 | 3592670 |
| thromboxane contributes to the immediate antigenic response of canine peripheral airways. | the actions of specific humoral mediators in the immediate response of the canine peripheral airways to antigen challenge are not well understood. using a method which allows localized exposure of the peripheral lung to antigen, we investigated the role of locally released thromboxane a2 (txa2) in the immediate response of collateral airways to aerosolized antigen. in dogs with native sensitivity to ascaris suum antigen, resistance to flow through the collateral system (rcs) was measured using a ... | 1987 | 3597229 |
| ascaris suum: human lymphocyte mitogenic factor content. | ascaris suum extract was found to contain a mitogenic factor which stimulates human lymphocytes. the extract (100 micrograms protein/ml) induced an increase in [3h]thymidine incorporation into human lymphocytes at a level similar to that obtained with pokeweed mitogen (11 micrograms/ml). stimulation of mitosis appeared to be more effective with t lymphocytes than non-t lymphocytes. the mitogenic activity of the extract was reduced by only 27% when treated at 56 c for 30 min or when immersed into ... | 1987 | 3609229 |
| detection of complement fixation antibody against ascaris suum antigen in pigs with white spots in the liver. | | 1987 | 3613356 |
| the electrophysiology of the somatic muscle cells of ascaris suum and ascaridia galli. | the electrophysiological properties of the bag region of the somatic muscle cells of ascaris suum and ascaridia galli were studied using intracellular techniques. for ascaris muscle cells, the mean resting membrane potentials at 20 and 37 degrees c were -29.9 and -33.8 mv respectively, and the average input conductance was 2.12 micros. for the muscle cells of a. galli similar values were obtained. for example, the mean input conductance of these cells was 2.84 micros at 20 degrees c. healthy asc ... | 1987 | 3614991 |
| [effects of various disinfectants on the development and survival possibilities of the pre-parasitic stages of ostertagia ostertagi, cooperia oncophora and ascaris suum]. | eleven disinfectants varying in chemical composition and containing active chlorine, phenol, cresol, sodium or potassium hydroxide, quaternary ammonium compounds, glutaraldehydes or paraformaldehyde were tested for their lethal effect on eggs and infective larvae of ostertagia ostertagi and cooperia oncophora and non-embryonated and embryonated eggs of ascaris suum. suspensions of all tested stages were mixed with the disinfectants in the concentrations recommended by the manufacturer and with d ... | 1987 | 3617021 |
| inhibition of malate dehydrogenase enzymes by benzimidazole anthelmintics. | determinations were made of the inhibitory activities of four benzimidazole anthelmintics (albendazole, parbendazole, mebendazole and thiabendazole) on purified extracts of cytoplasmic and mitochondrial malate dehydrogenase obtained from ascaris suum, fasciola hepatica and moniezia expansa. the highest percentage inhibitions were exhibited by mebendazole. the results confirm that cytoplasmic mdh and mitochondrial mdh regulator enzymes of glycogen synthesis are the sites of mebendazole inhibitory ... | 1987 | 3617430 |
| homology and heterology between the secreted antigens of the parasitic larval stages of ascaris lumbricoides and ascaris suum. | the materials released in vitro by the tissue-parasitic larval stages of the large roundworm of man, ascaris lumbricoides, were analysed by radio-iodination, immunoprecipitation, and sds-page. the antigens were found to be heterogeneous, ranging in molecular weight from 14 to 410 kd, and were found to alter radically during the parasites' migration to the lungs. the antigens secreted by the infective and lung-stage larvae of the pig homologue, ascaris suum, were compared with those of the human ... | 1987 | 3621674 |
| [comparative studies on slab-page of proteins of ascaris lumbricoides, ascaris suum and toxocara canis]. | | 1987 | 3623535 |
| retinal pigment epitheliopathy and neuroretinal degeneration in ascarid-infected eyes. | the generalized choroidal and retinal response to a focal nonreplicating infection of the eye with ascarid larvae was examined in an animal model. intravitreal injection of ascaris suum larvae in guinea pigs induced a diffuse eosinophilic choroiditis, retinal pigment epitheliopathy and neuroretinal degeneration, distant from focal reactions about larvae. as the choroiditis progressed, inflammatory cells separated the choriocapillaris from bruch's membrane, and the endothelial cells lost their fe ... | 1987 | 3623842 |
| anaerobic metabolism in ascaris suum: acyl coa intermediates in isolated mitochondria synthesizing 2-methyl branched-chain fatty acids. | freshly isolated ascaris suum mitochondria contained coash, acetyl coa, propionyl coa, 2-methylcrotonyl coa, 2-methylbutyryl coa, 2-methyl-2-pentenoyl coa, and 2-methylvaleryl coa, as determined by high-pressure liquid chromatography. incubation of these mitochondria aerobically in the absence of substrate resulted in the conversion of the branched-chain enoyl coa's to acetyl coa and propionyl coa. with the addition of malate to the incubation medium, succinyl coa and methylmalonyl coa accumulat ... | 1987 | 3627167 |
| efficacy of dichlorvos, fenbendazole, and ivermectin in swine with induced intestinal nematode infections. | anthelmintic efficacies of dichlorvos, fenbendazole, and ivermectin were compared in specific-pathogen-free crossbred weanling pigs inoculated with ascaris suum, trichuris suis, and oesophagostomum dentatum. on postinoculation day (pid) 50, 24 pigs in each treatment group were treated orally with 43 mg of dichlorvos/kg of body weight, 3 x 3 mg of fenbendazole/kg, or 300 micrograms of ivermectin/kg, sc. twenty-four pigs were not treated. on posttreatment day 7 (pid 57), 12 pigs from each treatmen ... | 1987 | 3631712 |
| [immunoenzyme (elisa) diagnosis of onchocerciasis: use of an antigenic fraction of ascaris suum]. | | 1987 | 3632075 |
| bronchoalveolar lavage and histologic characterization of late asthmatic response in guinea pigs. | to elucidate the mechanisms of late asthmatic response (lar) observed in asthmatic subjects, we have developed an animal model of lar using guinea pigs. fifty guinea pigs were immunized with a mixture of ascaris suum extract and aluminum hydroxide and then challenged with an inhalation of ascaris suum extract without anesthesia. twenty of the 50 guinea pigs showed a dual asthmatic response in which the lar occurred 3 to 6 h after immediate asthmatic response (iar). histologic studies by rapid fr ... | 1987 | 3662243 |
| superoxide dismutase activity in nematodes. | the activities of the enzymes superoxide dismutase (e.c.:1.15.1.1) and catalase (e.c.:1.11.1.6) were studied in purified extracts of four nematodes: ascaris suum, toxascaris leonina, toxocara canis and t. cati adult males and females. no catalase activity was found in any of the extracts. the results reveal that the sod activities of the four parasites presented species differences and also sexual differences within each species. polyacrylamide gel electrophoresis pattern analysis confirmed that ... | 1987 | 3668213 |
| evaluation of the effect of a platelet activating factor antagonist on platelet activating factor and ascaris antigen-induced airway responses in rhesus monkeys. | an acute airway response to aerosolized platelet activating factor (paf) can be induced in certain rhesus monkeys. this paf airway response is completely inhibited by the paf antagonist, sri 63-441, in normal monkeys. after the completion of these studies showing inhibition of the paf airway response, we evaluated whether the paf antagonist could inhibit three types of acute ige-mediated airway responses to ascaris suum (a) in rhesus monkeys. the a aerosol test protocols used the following: a si ... | 1987 | 3668360 |
| identity and tissue localization of free and conjugated ecdysteroids in adults of dirofilaria immitis and ascaris suum. | adult males and females of the dog heartworm, dirofilaria immitis, and of the swine parasite, ascaris suum, were extracted, the free and polar conjugated ecdysteroid fractions separated and the latter hydrolysed enzymically. the ecdysteroids released by hydrolysis of the conjugates and the free hormones were analysed by radioimmunoassay, high-performance liquid chromatography on reversed phase and adsorption columns monitoring fractions by radioimmunoassay, and by gas-liquid chromatography/mass ... | 1987 | 3670345 |
| effects of a 5-lipoxygenase inhibitor (l-651,392) on primary and late pulmonary responses to ascaris antigen in the squirrel monkey. | allergic squirrel monkeys when exposed to an aerosol of ascaris suum either develop a reproducible, immediate bronchoconstriction or an immediate bronchoconstriction followed by a reproducible late response. pretreatment of ascaris-challenged squirrel monkeys with a potent, selective, orally active 5-lipoxygenase inhibitor, l-651,392 (4-bromo-2,7-dimethoxy-3,4-phenothizin-3-one), at a dose of 5 mg/kg p.o. resulted in near complete inhibition of the increases in pulmonary resistance (rl) and decr ... | 1987 | 3687598 |
| late asthmatic response to ascaris antigen challenge in dogs treated with metyrapone. | we developed an experimental dog preparation that shows a biphasic bronchoconstriction after allergen exposure. after anesthetization, the dogs were intubated with endotracheal tubes and manually ventilated. respiratory resistance (rrs) was measured by the forced oscillation method at 3 hz. ascaris suum, diluted from 10(-5) to 10(-2) of the extract, was inhaled during tidal breathing for 5 min. one hour before antigen challenge, 10 dogs received 70 mg/kg of metyrapone (cortisol synthesis inhibit ... | 1987 | 3688649 |
| effects of sobrerol treatment on macromolecule secretion, ion and water fluxes in sheep trachea. | we studied the effects of sobrerol treatment on airway mucus secretion. normal sheep and sheep with airways sensitized to ascaris suum antigen were treated in vivo with sobrerol, 1 mg.kg-1 i.m., twice a day for five days. composition and secretion of mucus macromolecules, and fluxes of ions and water were subsequently measured in tracheal tissues in vitro, and were compared to values from untreated (normal and sensitized) sheep. macromolecules were radiolabelled with 35so4 and 3h-threonine and w ... | 1987 | 3690026 |
| suppressive effect of an ascaris suum extract on ige and igg antibody responses in mice. | 1. a suppressed cytotropic and agglutinating antibody response against ovalbumin was induced in mice immunized with this antigen and an ascaris suum extract. 2. suppression of ige antibody production was abolished by administration of cyclophosphamide or x-irradiation before immunization. in contrast, suppression of homocytotropic igg1 and heterocytotropic igg2a antibody responses was considerably resistant to the same treatments. 3. the low levels of igg agglutinating antibodies also remained u ... | 1987 | 3690055 |
| parasite transmission in confined hogs. | examinations of fecal specimens from swine of all ages maintained in a totally enclosed confinement facility in south georgia were conducted in a 5-year survey (1977-1981) to determine the prevalence, intensity, and transmission patterns of intestinal nematode and protozoan parasites. weaned pigs in the nursery had no detectable parasitic infections except sporadic isospora suis in newly weaned pigs. growing-finishing hogs had a low prevalence of ascaris suum, oesophagostomum spp. and balantidiu ... | 1986 | 3705423 |
| [effect of selected pesticides on the development and invasiveness of the ova of ascaris suum goeze, 1782]. | | 1986 | 3705585 |
| the effect of antigen-induced bronchoconstriction on phrenic nerve activity. | the effects of acute bronchoconstriction, produced by inhalation of ascaris suum antigen, on both the amplitude and timing of phrenic nerve activity were studied in anesthetized dogs. blood gas tensions and inspiratory air flow were maintained constant. bronchoconstriction resulted in a significant increase in the magnitude and rate of rise of the phrenic neurogram during both normal respiratory cycles and cycles when lung inflation was prevented. the increase in the slope of the phrenic neurogr ... | 1986 | 3706882 |
| differences in airway responsiveness to leukotriene d4 in allergic sheep with and without late bronchial responses. | allergic sheep respond to inhaled ascaris suum antigen with either acute and late bronchial obstructions (dual responders) or only acute bronchoconstriction (acute responders). in this study we tested the hypothesis that one factor which may distinguish between these two populations is the difference in sensitivity to a specific mediator of airway anaphylaxis, leukotriene (lt) d4 (a major component of slow reacting substance of anaphylaxis). we postulated that if the hypothesis was correct then ... | 1986 | 3715056 |
| [influence of the migration of ascaris suum larvae on blood parameters in the pig]. | biochemical and hematological blood parameters were investigated in pigs experimentally infected with ascaris suum. the most marked changes were recorded on day 5-8 post inoculation, during fever. in this period there was a marked decrease of concentrations of albumin, iron, thyroxin, a and e vitamins in blood serum, while compared with the control group the contents of a vitamin in liver did not change. further, the concentrations of nonesterified fatty acid increased, as well as those of inorg ... | 1986 | 3717687 |
| ascaris suum infection in pigs sensitizes lymphocytes but suppresses their responsiveness to phytomitogens. | the effect of ascaris suum infection and treatment with fenbendazole on the blastogenic response of peripheral blood lymphocytes to a. suum antigens and to three phytomitogens was assayed by the lymphocyte transformation technique. repeated infections with a. suum led to the development of sensitized lymphocytes primarily responding to egg hatching fluid antigen. treatment with fenbendazole decreased the number of specific sensitized lymphocytes, but favorably increased the resistance of pigs to ... | 1986 | 3727343 |
| 31p-nmr studies of the metabolisms of the parasitic helminths ascaris suum and fasciola hepatica. | 31p-nmr has been applied to the study of the metabolisms of the intact parasitic helminths ascaris suum (the intestinal roundworm) and fasciola hepatica (the liver fluke). after calibration of the chemical shift of pi in muscle extracts the internal ph of adult ascaris worms and the effect of the ph of the external medium on the organism's internal ph were measured. assignments of nearly all of the observable 31p resonances could be made. a large resonance from glycerophosphorylcholine whose fun ... | 1986 | 3729415 |
| ph dependence of kinetic parameters for oxalacetate decarboxylation and pyruvate reduction reactions catalyzed by malic enzyme. | both chicken liver nadp-malic enzyme and ascaris suum nad-malic enzyme catalyze the metal-dependent decarboxylation of oxalacetate. both enzymes catalyze the reaction either in the presence or in the absence of dinucleotide. the presence of dinucleotide increases the affinity of oxalacetate for the chicken liver nadp-malic enzyme, but this information could not be obtained in the case of a. suum nad-malic enzyme because of the low affinity of free enzyme for nad. the kinetic mechanism for oxalac ... | 1986 | 3741834 |
| chitin synthesis in zygotes of ascaris suum. | in ascaris suum chitin is formed in the zygote immediately after oocyte fertilization, and its synthesis is completed in the eggs from the distal half of the uterus. incorporation of radiocarbon [14c] glucose into chitin of the eggshell was 40-fold higher than incorporation of [14c] glucosamine. the same rank order also holds for the incorporation of label from these isotopes into the glycogen of the ovaries. a large part of the radiolabel was incorporated first into oocyte glycogen and only aft ... | 1986 | 3745873 |
| susceptibility of fourth-stage ascaris suum larvae to fenbendazole and to host response in the pig. | fenbendazole given at the rate of 2.5 g/kg of feed for 3 days had 100% efficacy against 4th-stage ascaris suum larvae in 8 pigs. eight control pigs had a total of 108 a suum. in 6 pigs infected 3 times with 3rd-stage a suum larvae and treated with fenbendazole after the larvae molted to the 4th stage, the challenge exposure-derived population was decreased by 64%. similar sequential infections in 6 pigs similarly infected, but not treated with fenbendazole, decreased the challenge exposure-deriv ... | 1986 | 3752674 |
| electron-transfer complexes of ascaris suum muscle mitochondria. ii. succinate-coenzyme q reductase (complex ii) associated with substrate-reducible cytochrome b-558. | a succinate-coenzyme q reductase (complex ii) was isolated in highly purified form from ascaris muscle mitochondria by detergent solubilization, ammonium sulfate fractionation and gel filtration on a sephadex g-200 column. the enzyme preparation catalyzes electron transfer from succinate to coenzyme q1 with a specific activity of 1.2 mumol coenzyme q1 reduced per min per mg protein at 25 degrees c. the isolated complex ii is essentially free of nadh-ferricyanide reductase, reduced coq2-cytochrom ... | 1986 | 3753651 |
| the effect of an orally active leukotriene d4/e4 antagonist, ly171883, on antigen-induced airway responses in allergic sheep. | leukotriene (lt) d4 is a putative mediator of allergic asthma: inhaled ltd4 produces early and late increases in specific lung resistance (srl) and slows tracheal mucus velocity (tmv) similar to inhaled antigen. in this study we examined the effects of an orally active ltd4/lte4 antagonist, ly171883 [1-less than 2-hydroxy-3-propyl-4-less than 4-(1h-tetrazol-5-yl) butoxy greater than phenyl greater than ethanone], on early and late changes in srl and tmv following airway challenge with ascaris su ... | 1986 | 3754973 |
| the effect of pre-farrowing treatment of the sow with ivermectin on the early establishment of ascaris suum and oesophagostomum spp in the progeny. | residual infective ascaris suum eggs in farrowing pens provide the initial source of infection for piglets under swedish management conditions. in such circumstances there is little evidence to support pre-farrowing treatment of the sow specifically against a. suum. the present study shows, however, that ivermectin-treatment of the sow prevents the early establishment of a. suum and oesophagostomum spp in her litter. | 1986 | 3755816 |
| some precursors of glycogen in ascaris suum females. | intensity of incorporation of the labelled carbon from glucose, glucosamine and leucine into the glycogen of somatic cells and reproductive organs of ascaris suum females has been studied. glucose was most intensively incorporated into the growing oocytes and into the uterine epithelial cells, whereas its incorporation into muscle glycogen was found to result in lowest rates. radiocarbons from glucosamine and leucine were incorporated into tissue glycogen of female worms much less than glucose. ... | 1986 | 3758868 |
| purification and characterization of phosphoenolpyruvate carboxykinase from the parasitic helminth ascaris suum. | phosphoenolpyruvate carboxykinase has been purified from homogenates of ascaris suum muscle strips to apparent homogeneity as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the purification is a three-step procedure which yields pure enzyme in milligram quantities with good yield. the subunit molecular weight of the ascaris enzyme is between 75,000 and 80,000. the native molecular weight is 83,000 as determined by gel filtration. the kinetic constants for substrates of ... | 1986 | 3759946 |
| effect of an inhaled glucocorticosteroid (budesonide) on post-antigen induced increases in airway responsiveness. | we examined the effects of an inhaled glucocorticosteroid, budesonide, on antigen-induced early and late bronchial responses and the development 24 h after challenge of increased airway responsiveness to carbachol in allergic sheep. six allergic sheep were used for this study and, on occasions separated by at least three weeks, they were treated: with placebo (treatment 1); 16 h and 20 min prior to antigen challenge with budesonide (treatment 2); 16 h and 20 min prior to and 8 h after antigen ch ... | 1986 | 3768572 |
| [quantitative comparison of amino acids in adult proteins of ascaris lumbricoides, ascaris suum and toxocara canis]. | | 1986 | 3770717 |
| peripheral circulatory alterations in canine anaphylactic shock. | we have examined peripheral circulatory variables that might contribute to the decrease in cardiac output and arterial pressure characteristic of anaphylactic shock. in six dogs instrumented with a right heart bypass, the intravenous administration of ascaris suum antigen caused a 53% decrease in cardiac output and a 58% decrease in arterial pressure. resistance to venous return increased from 0.0038 +/- 0.0004 to 0.0056 +/- 0.0006 mmhg x ml-1 x min (p less than 0.05), mean systemic pressure dec ... | 1986 | 3777201 |
| the bacterial flora of the intestine of ascaris suum and 5-hydroxytryptamine production. | representative facultative anaerobes of the bacterial flora from the intestine of female ascaris suum were isolated and identified. the number of bacteria in the intestine was approximately 4 x 10(9) per g wet weight of intestine. seventeen of 19 of the isolated colonies were found to secrete 5-hydroxytryptamine in culture. holding a. suum in an antibiotic-containing medium did not affect the levels of 5-hydroxytryptamine in the worm, which were 231 +/- 14 ng/g in antibiotic-media as compared to ... | 1986 | 3783348 |
| improved purification of the pyruvate dehydrogenase complex from ascaris suum body wall muscle and characterization of pdha kinase activity. | an improved purification scheme for the isolation of the ascaris suum pyruvate dehydrogenase complex directly from body wall muscle has been developed which yields a fully activated pyruvate dehydrogenase complex with substantial pdha kinase activity. the apparent km for coenzyme a (coa) is much lower than previously reported and can only be accurately measured in the presence of a coa-regenerating system. the alpha-pyruvate dehydrogenase subunit of the ascarid complex is unique and its migratio ... | 1986 | 3785292 |
| micromotility meter: an instrument designed to evaluate the action of drugs on motility of larval and adult nematodes. | an instrument for measuring the motility of larval and adult nematodes is described along with an analysis of its use as a tool to analyse drug action on these parasites. motility was detected from larval parasites of nippostrongylus brasiliensis and ascaris suum in the absence and presence of various anti-nematodal drugs. these agents produced, within 48 h, a significant decrease in larval parasite motility. the instrument was also capable of detecting the motility of caenorhabditis elegans, ad ... | 1986 | 3785972 |
| immunity of swine to ascaris suum. | swine were hyperimmunized to ascaris suum by giving multiple oral inoculations of embryonated eggs. sera and lymphocyte lysate from these pigs were administered parenterally to 4-week-old pigs. the latter animals were no more resistant to larval migration than control pigs receiving sera or lymphocyte lysate from non-immunized pigs. other pigs were infected with transmissible gastroenteritis (tge) virus, allowed to recover and challenged with embryonated ascarid eggs. they likewise were no more ... | 1986 | 3788028 |
| [preliminary karyotype studies on ascaris lumbricoides and ascaris suum from guangzhou]. | | 1986 | 3791592 |
| phosphorylcholine bearing components of some helminthic parasites: localization to parasite lipoproteins. | extracts of various helminthic parasites were studied to determine the molecular carrier of the hapten phosphorylcholine (pc). physicochemical studies showed that the carrier was heterogeneous with regard to apparent molecular weight (70,000-greater than 10(6) daltons). ultracentrifugal analysis of ascaris suum and nippostrongylus brasiliensis showed that pc was associated with low (ldl), high (hdl) and a heterogeneous group of very high density (vhdl) lipoproteins. the majority of pc was found ... | 1986 | 3791963 |
| preliminary communication on cryptosporidiosis of pigs in viet-nam. | feces were collected from 17 pigs (aged 4-8 weeks) in several farms situated within the region of hanoi. by coproscopy of the samples, eggs of ascaris suum and oesophagostomum dentatum were detected as well as oocysts of eimeria scabra, e. debliecki, e. perminuta and e. suis, and in addition to it, cryptosporidia to an extend indicating a considerable incidence of this parasitic protozoan in the country. | 1986 | 3804081 |
| an immunochemical investigation of the allergens from ascaris suum perienteric fluid. cross-reactivity, molecular weight distribution and correlation with phosphorylcholine-containing components. | an extract of ascaris suum perienteric fluid was fractionated on sephadex g-200 and the resulting high molecular weight (approx. 600,000 daltons) and low molecular weight (approx. 16,000 daltons) fractions examined for allergenicity using human sera. radioallergosorbent (rast) and rast-inhibition assays showed that the high molecular weight fraction was 15- to 40-fold more potent than the low molecular weight fraction. crossed radio-immunoelectrophoretic analysis showed that the high molecular w ... | 1987 | 3804458 |
| late pulmonary responses induced by ascaris allergen in conscious squirrel monkeys. | this study presents an antigen-dependent model of biphasic pulmonary changes to ascaris suum in conscious squirrel monkeys. animals with strong positive skin reactivity towards a. suum were trained to sit quietly in chairs and to breathe through face masks. dynamic compliance (cdyn) and pulmonary resistance (rl) were measured in these conscious animals before and for a period of 11 h after administration of an aerosol of ascaris or ragweed antigen. the aerosol of ascaris antigen induced reproduc ... | 1986 | 3804916 |
| adenylate cyclase activity is absent in inactive and motile sperm in the nematode parasite, ascaris suum. | | 1986 | 3819975 |
| collection of fertile ascaris suum eggs. | | 1986 | 3819976 |
| effect of amoscanate derivative cgp 8065 on aminoacyl-trna synthetases in ascaris suum. | evidence is provided for the occurrence of a multienzyme complex consisting of several aminoacyl-trna synthetases besides 'free enzymes' in ascaris suum. the molecular mass of this complex was calculated to be about 10(6) daltons, compared to about 150 000 daltons for the seryl-trna synthetase. leucyl-, isoleucyl-, arginyl- and lysyl-trna synthestases were found in the high molecular weight fraction. the michaelis constants of these aminoacyl-trna synthetases were found to be in the range of 4 t ... | 1985 | 3838573 |
| calcium-dependent muscle contraction in obliquely striated ascaris suum muscle. | the regulatory proteins of ascaris suum striated skeletal muscle were partially purified and characterized. a tropomyosin isoform (mr 41k) and three troponin subunits identified as troponin t (mr 37.5k), troponin i (mr 25.5k) and troponin c (mr 18.5k) were purified. three myosin light chains (mr 25k, 19k, and 17k) were isolated from washed ascaris actomyosin; the 19k subunit was phosphorylated in vitro. a calcium/calmodulin-dependent myosin light chain kinase activity was identified in the muscl ... | 1985 | 3840425 |
| parasite-specific interaction of n-[4-(4'nitroanilino)-phenyl]-s-(beta-carboxyethyl)-dithiocar bamic acid-ester with arginyl-trna-synthetase from dirofilaria immitis. | a potent inhibition of arginyl-trna-synthetase from dirofilaria immitis by n-[4-(4'nitroanilino)-phenyl]-s-(beta-carboxylethyl)-dith iocarbamic acid-ester (cgp 8065) is demonstrated, which indicates the charging of amino acids to trna as a possible target for the chemotherapeutic attack by this filaricidal compound. cgp 8065 competes with atp; the inhibition constant was determined to be 13 micrograms ml-1 (34 microm). the michaelis constants for arginine and atp were found to be 5 microm and 0. ... | 1985 | 3841408 |
| ascaris suum: biosynthesis and isoinhibitor profile of chymotrypsin/elastase isoinhibitors. | chymotrypsin/elastase isoinhibitors were radiolabeled when live, adult ascaris suum were incubated in tissue culture medium (nctc-135) supplemented with l-[35s]cysteine. this is the first demonstration that the synthesis of these proteins occurs in a. suum; the isoinhibitors are not host products utilized by the parasite against its host. of five chymotrypsin/elastase isoinhibitors demonstrable in a. suum, only isoinhibitors 1, 4, and 5 were found in each worm. the amino acid sequences of these ... | 1985 | 3846534 |
| changes in the white blood picture during experimental larval ascariasis, toxocariasis and toxascariasis. | haematological changes were studied in rabbits experimentally infected with different doses of ascaris suum, toxacara canis and toxascaris leonina. the numbers of leucocytes, eosinophiles and neutrophiles were changed and the strongest eosinophila was observed between the 2nd and 3rd week of infection (5.2-7.1%). the intensity of reaction was not related to the used infection dose. the eosinophilia was always lower in toxocariasis and toxascariasis than in ascariasis at identical doses of infect ... | 1985 | 3862632 |
| effect of repeated antigen exposure on antigen-and mediator-induced bronchospasm in sheep. | we studied the effects of repeated exposures of antigen on airway reactivity to mediators of anaphylaxis and immediate response to the antigen. seven antigen-sensitive sheep were exposed to aerosols of ascaris suum antigen 5 times biweekly; a control group of seven sheep underwent the same exposure regimen with saline vehicle. sheep were assigned to experimental (ascaris) or control groups so the distribution of animals with regard to bronchial reactivity to mediators was about the same. airway ... | 1985 | 3865925 |
| antigen sensitization and methacholine responses in dogs with hyperreactive airways. | antigen sensitization was induced in six basenji-greyhound (bg) dogs by weekly aerosol exposure to ascaris suum. the effects on airway responsiveness to inhaled methacholine were studied before and at least 2 wk following ascaris sensitization. all dogs developed detectable serum levels of ascaris-specific immunoglobulin e (ige), and five out of six dogs developed airway responsiveness to antigen over the 4- to 6-mo period. this was accompanied by a decrease rather than an increase in airway res ... | 1985 | 3884573 |