| prevalence of helminthic infections among inhabitants of lao pdr. | the prevalence of helminthic infections was surveyed on a total of 6,178 residents (males 2,549 and females 3,629) in 102 villages of 9 provinces in lao pdr between 2007 and 2011 under the project of korea-laos collaborative project for control of foodborne trematode infections in lao pdr. fecal specimens were collected and examined by the kato-katz thick smear and stoll's egg counting techniques. the overall liver/intestinal helminth egg positive rate was 71.9% with a single or mixed infections ... | 2014 | 24623882 |
| helminth infection in southern laos: high prevalence and low awareness. | helminthiasis is a public health concern in lao people's democratic republic (lao pdr, laos). this study aimed to understand helminth infection and associated risk factors in relation to existing local knowledge, attitudes and practices regarding worm infections in endemic communities. | 2013 | 24499584 |
| Raw attitudes, wetland cultures, life-cycles: Socio-cultural dynamics relating to Opisthorchis viverrini in the Mekong Basin. | Opisthorchis viverrini is one of the most common and medically important food-borne parasites in the Lower Mekong area of Southeast Asia. As we learn more about its ecology, pathology and epidemiology we see the need to consider more deeply the socio-cultural dynamics with which food-borne species complexes are associated. This paper argues that the Mekong region is characterized by strong livelihoods and life-style associations within wetland ecosystems, which are inseparable from human eating ... | 2011 | 21712097 |
| high prevalence of opisthorchis viverrini infection in a riparian population in takeo province, cambodia. | opisthorchis viverrini infection was found to be highly prevalent in 3 riverside villages (ang svay chek a, b, and c) of the prey kabas district, takeo province. this area is located in the southern part of cambodia, where the recovery of adult o. viverrini worms was recently reported. from may 2006 until may 2010, fecal examinations were performed on a total of 1,799 villagers using the kato-katz thick smear technique. in the 3 villages, the overall positive rate for helminth eggs ranged from 5 ... | 2012 | 22711932 |
| similarity of a 16.5kda tegumental protein of the human liver fluke opisthorchis viverrini to nematode cytoplasmic motility protein. | opisthorchis viverrini is the causative agent of human opisthorchiasis in thailand and long lasting infection with the parasite has been correlated with the development of cholangiocarcinoma. in this work we have molecularly characterized the first member of a protein family carrying two dm9 repeats in this parasite (ovdm9-1). interpro and other protein family databases describe the dm9 repeat as a protein domain of unknown function that has been first noted in drosophila melanogaster. two paral ... | 2016 | 27140280 |
| timps of parasitic helminths - a large-scale analysis of high-throughput sequence datasets. | tissue inhibitors of metalloproteases (timps) are a multifunctional family of proteins that orchestrate extracellular matrix turnover, tissue remodelling and other cellular processes. in parasitic helminths, such as hookworms, timps have been proposed to play key roles in the host-parasite interplay, including invasion of and establishment in the vertebrate animal hosts. currently, knowledge of helminth timps is limited to a small number of studies on canine hookworms, whereas no information is ... | 2013 | 23721526 |
| genome mining offers a new starting point for parasitology research. | parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ... | 2015 | 25563615 |
| [role of infectious agents in the emergence of malignant tumors]. | according to the data of the international agency for research on cancer (iarc), at least 6 virus species (hpv, ebv, hhv-8/kshv, htlv-1, hbv, hcv), 4 helminthes species (schistosoma haematobium and japonicum, opisthorchis viverrini, clonorchis sinensis) and i bacterium species (helicobacter pylori) have been proved to be capable of causing the development of cancer. the analysis of the data available shows that merkel cell polyomavirus (mcv), herpes simplex virus (hsv), john cunningham polyomavi ... | 2012 | 23163048 |
| a cross-sectional study on intestinal parasitic infections in rural communities, northeast thailand. | despite the existence of effective anthelmintics, parasitic infections remain a major public health problem in southeast asia, including thailand. in rural communities, continuing infection is often reinforced by dietary habits that have a strong cultural basis and by poor personal hygiene and sanitation. this study presents a survey of the prevalence of intestinal parasitic infections among the people in rural thailand. the community-based cross-sectional study was conducted in villages in khon ... | 2013 | 24516280 |
| a hospital-based study of epidemiological and clinical data on blastocystis hominis infection. | blastocystis hominis is a foodborne protozoan found in the human feces worldwide. one hundred and ninety-nine individuals with stool samples positive for b. hominis were identified from a pool of 14,325 patient stools collected between 2003 and 2010 from srinagarind hospital in thailand. the medical records of patients were reviewed for demographic and clinical data. of the 85 patients (42.7%) who had b. hominis infection with no co-infections, 42.5% experienced gastrointestinal symptoms. abdomi ... | 2012 | 23075461 |
| Diagnostic values of parasite-specific antibody detections in saliva and urine in comparison with serum in opisthorchiasis. | Infection by the liver fluke (Opisthorchis viverrini) causes hepatobiliary disease and bile duct cancer (cholangiocarcinoma, CCA) in endemic areas in Southeast Asia. Measurements of humoral immune response particularly parasite-specific antibodies are useful not only for serodiagnosis but they have been implicated as risk factors of CCA. In this study, we used indirect Enzyme Immunosorbent Assay (ELISA) to measure O. viverrini-specific immunoglobulins in serum, urine and saliva and assessed effi ... | 2011 | 21704727 |
| Situation Analysis of Risk Factors Related to Non-communicable Diseases in Khon Kaen Province, Thailand. | A descriptive cross-sectional study was carried out in Khon Kaen Province during January 1 to June 30, 2008. The aims were to assess: (1) the prevalence of risk factors for chronic diseases such as cancer, diabetes, blood pressure; and (2) health behaviour and health education needs. There were 338 sample subjects aged between 20-60 years, from urban, semi-urban and rural areas. Some 20.4 % of the sample subjects reported that they were unhealthy (10.4% diagnosed with hypertension, 9.8% with dia ... | 2011 | 21875293 |
| genome size estimation of liver fluke opisthorchis viverrini by real-time polymerase chain reaction based method. | the human liver fluke, opisthorchis viverrini, has been categorized as a class one carcinogenic organism according to its strong association with cholangiocarcinoma, bile duct cancer which has high incidence in the northeast of thailand. the lack of genome database of this parasite limited the studies aimed to understand the basic molecular biology of this carcinogenic liver fluke. the determination of the genome size is an initial step prior to the full genome sequencing. in this study, we appl ... | 2011 | 21757028 |
| hyperendemicity of haplorchis taichui infection among riparian people in saravane and champasak province, lao pdr. | in this study, we found that haplorchis taichui, a heterophyid intestinal fluke, is highly prevalent, with heavy worm loads, among riparian people in saravane and champasak province, lao pdr. fecal specimens were collected from 1,460 people (717 men and 743 women) in 12 riparian (mekong river) districts and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 78.8% and 66.4% in saravane and champasak province, respectively. the positive rate for small ... | 2013 | 23864741 |
| hepatic cytochrome p450 2a6 and 2e1 status in peri-tumor tissues of patients with opisthorchis viverrini-associated cholangiocarcinoma. | endogenous nitrosation due to chronic inflammation is enhanced in opisthorchiasis and plays a crucial role in the development of cholangiocarcinoma (cca). hepatic cytochrome p450 (cyp) family enzymes, especially cyp2a6 and cyp2e1, are involved in the metabolism of procarcinogens; these two enzymes metabolize endogenous nitrosamines to carcinogenic n-dimethylnitrosamine (ndma). cyp2a6 activity is increased in patients infected with opisthorchis viverrini. our aim was to determine whether the expr ... | 2011 | 21745589 |
| a cross-sectional study on the potential transmission of the carcinogenic liver fluke opisthorchis viverrini and other fishborne zoonotic trematodes by aquaculture fish. | throughout southeast asia and china, eating raw and or partially cooked cyprinid fish causes liver (hepatobiliary) disease and cancer (cholangiocarcinoma) due to fishborne zoonotic trematodes (fzt), in particular clonorchis sinensis and opisthorchis viverrini. the primary source of transmission is by native fish, but aquaculture fish are also reported to have high infective potential. here, a cross-sectional survey of fzt in fish farms was conducted in an endemic area in khon kaen province, thai ... | 2013 | 23320421 |
| high prevalence of opisthorchis viverrini infection in reservoir hosts in four districts of khon kaen province, an opisthorchiasis endemic area of thailand. | khon kaen, a northeastern province of thailand, has been considered as one of the human opisthorchiasis endemic areas with continuing high prevalence. unsuccessful eradication of the disease is probably from the culture of eating raw and undercooked fish of local residence and the parasitic persistency in animal reservoir hosts, such as cats and dogs. in cooperation with the other human opisthorchiasis control programs in an endemic area of 29 villages in ban haet, ban phai, chonnabot and muncha ... | 2011 | 21843654 |
| cytokine/chemokine secretion and proteomic identification of upregulated annexin a1 from peripheral blood mononuclear cells cocultured with the liver fluke opisthorchis viverrini. | we investigated the cytokine/chemokine secretions and alteration of protein expression from peripheral blood mononuclear cells (pbmcs) cocultured with adult liver flukes (opisthorchis viverrini) for 6 to 24 h. pbmc-derived proteins were identified by two-dimensional electrophoresis and mass spectrometry, and the cytokines/chemokines in the supernatant were assessed using a cytokine array. exposure to o. viverrini induced increases in secretion of proinflammatory cytokines, costimulating protein, ... | 2014 | 24614660 |
| proteomic identification of plasma protein tyrosine phosphatase alpha and fibronectin associated with liver fluke, opisthorchis viverrini, infection. | opisthorchiasis caused by opisthorchis viverrini induces periductal fibrosis via host immune/inflammatory responses. plasma protein alteration during host-parasite interaction-mediated inflammation may provide potential diagnostic and/or prognostic biomarkers. to search for target protein changes in o. viverrini-infected hamsters, a 1-d page gel band was trypsin-digested and analyzed by a lc-ms/ms-based proteomics approach in the plasma profile of infected hamsters, and applied to humans. sixty ... | 2012 | 23029023 |
| clonorchis sinensis excretory-secretory products promote the migration and invasion of cholangiocarcinoma cells by activating the integrin β4-fak/src signaling pathway. | cholangiocarcinoma (cca) is a slow-growing but highly metastatic cancer. its metastatic potential largely explains its high mortality rate. a recognized risk factor for cca development is infection with the liver flukes opisthorchis viverrini and clonorchis sinensis. we previously reported that the excretory-secretory products (esps) of c. sinensis promoted the three-dimensional aggregation and invasion of cca cells. in the present study, a quantitative real-time pcr array of extracellular matri ... | 2017 | 28286026 |
| prevalence of gastrointestinal helminth parasites of zoonotic significance in dogs and cats in lower northern thailand. | gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. we investigated the prevalence of gastrointestinal helminths of zoonotic significance in dogs and cats in lower northern thailand and utilized molecular tools for species identification of hookworms and opisthorchis viverrini. fecal samples of 197 dogs and 180 cats were collected. overall prevalence of infection using microscopy was 40.1% in dogs and 33.9% in cats. helminth infection found in both dogs a ... | 2017 | 27570099 |
| comparative evaluation of strongyloides ratti and s. stercoralis larval antigen for diagnosis of strongyloidiasis in an endemic area of opisthorchiasis. | the use of strongyloides ratti as heterologous antigen for serodiagnosis of strongyloidiasis is preferable to strongyloides from humans due to the ease and safety of antigen preparation. in southeast asia where opisthorchis viverrini coexists with strongyloides stercoralis, there has been no report in using s. ratti for serodiagnosis of s. stercoralis. in this study, performance of an enzyme-linked immunosorbent assay (elisa) based on s. ratti was compared with that based on s. stercoralis for d ... | 2015 | 25877389 |
| clonorchis sinensis, an oriental liver fluke, as a human biological agent of cholangiocarcinoma: a brief review. | parasitic diseases remain an unarguable public health problem worldwide. liver fluke clonorchis sinensis is a high risk pathogenic parasitic helminth which is endemic predominantly in asian countries, including korea, china, taiwan, vietnam, and the far eastern parts of russia, and is still actively transmitted. according to the earlier 8th national survey on the prevalence of intestinal parasitic infections in 2012, c. sinensis was revealed as the parasite with highest prevalence of 1.86% in ge ... | 2016 | 27418285 |
| epidemiology of strongyloides stercoralis on mekong islands in southern laos. | strongyloides stercoralis is a neglected helminth infection potentially that can lead to systemic infection in immunocompromised individuals. in lao people's democratic republic (lao pdr, laos), information on s. stercoralis infection is scarce. we assessed s. stercoralis infection and associated risk factors and symptoms on the mekong islands in southern laos. baermann and kato-katz techniques were performed on two stool samples from each individual to detect s. stercoralis larvae and concomita ... | 2015 | 25291044 |
| characterization and localization of opisthorchis viverrini fructose-1,6-bisphosphate aldolase. | opisthorchis viverrini (ov) infection is a long-time public health problem in thailand that can lead to bile duct cancer, cholangiocarcinoma (cca). characterization of the ov proteins at a molecular level will increase our knowledge of host-parasite interaction that can be applied to new drug, vaccine, or immunodiagnostic development. in this study, an important enzyme in the ov glycolytic pathway, fructose-1,6-bisphosphate aldolase (fbpa), that had been obtained from a previous study was charac ... | 2016 | 27265876 |
| repeated stool sampling and use of multiple techniques enhance the sensitivity of helminth diagnosis: a cross-sectional survey in southern lao people's democratic republic. | intestinal parasitic infections are common in lao people's democratic republic (lao pdr). we investigated the accuracy of the kato-katz (kk) technique in relation to varying stool sampling efforts, and determined the effect of the concurrent use of a quantitative formalin-ethyl acetate concentration technique (fect) for helminth diagnosis and appraisal of concomitant infections. the study was carried out between march and may 2006 in champasack province, southern lao pdr. overall, 485 individual ... | 2015 | 25225157 |
| carcinogenesis associated with parasites other than schistosoma, opisthorchis and clonorchis: a systematic review. | only three helminths (schistosoma haematobium, opisthorchis viverrini and clonorchis sinensis) are directly associated with carcinogenesis in humans whereas the role of other parasites in cancer remains unclear. this study aimed to perform a systematic review to identify recent insights in the role of other parasite infections in carcinogenesis. we conducted systematic searches of medline and embase on july 2015. our primary outcome was the association between parasitic infections and carcinogen ... | 2016 | 26840624 |
| advances with the chinese anthelminthic drug tribendimidine in clinical trials and laboratory investigations. | the anthelminthic drug tribendimidine has been approved by chinese authorities for human use in 2004, and a first comprehensive review was published in acta tropica in 2005. here, we summarise further advances made through additional clinical trials and laboratory investigations. two phase iv trials have been conducted in the people's republic of china, the first one enrolling 1292 adolescents and adults aged 15-70 years and the second one conducted with 899 children aged 4-14 years who were inf ... | 2013 | 23352956 |
| prevalence of trematode larvae in intermediate hosts: snails and fish in ko ae sub-district of khueang nai, ubon ratchathani province, thailand. | ko ae sub-district of khueang nai, ubon ratchathani province, thailand is located in an endemic area for opisthorchis viverrini and other fish-borne zoonotic trematodes (fzt) infection. this study shows the status in ko ae sub-district of fzt infection based on availability of intermediate hosts and necessary requirements for the transmission of fzt. a cross-sectional survey of intermediate hosts of fzt, including bithynia siamensis goniomphalos and cyprinoid fish, was conducted from april 2013 ... | 2016 | 27405122 |
| infection status of zoonotic trematode metacercariae in fishes from vientiane municipality and champasak province in lao pdr. | the infection status of fishborne zoonotic trematode (fzt) metacercariae was investigated in fishes from 2 localities of lao pdr. total 157 freshwater fishes (17 species) were collected in local markets of vientiane municipality and champasak province in december 2010 and july 2011, and each fish was examined by the artificial digestion method. total 6 species of fzt metacercariae, i.e., opisthorchis viverrini, haplorchis taichui, haplorchis yokogawai, haplorchis pumilio, centrocestus formosanus ... | 2015 | 26323843 |
| zoonotic trematode metacercariae in fish from phnom penh and pursat, cambodia. | a survey was performed to investigate the infection status of freshwater fish with zoonotic trematode metacercariae in phnom penh and pursat province, cambodia. all collected fish with ice were transferred to our laboratory and examined using the artificial digestion method. in fish from phnom penh, 2 kinds of metacercariae (opisthorchis viverrini and haplorchis yokogawai) were detected. o. viverrini metacercariae were positive in 37 (50.0%) of 74 fish in 11 species (average no. metacercariae/fi ... | 2014 | 24623879 |
| Liver fluke-induced hepatic oxysterols stimulate DNA damage and apoptosis in cultured human cholangiocytes. | Oxysterols are cholesterol oxidation products that are generated by enzymatic reactions through cytochrome P450 family enzymes or by non-enzymatic reactions involving reactive oxygen and nitrogen species. Oxysterols have been identified in bile in the setting of chronic inflammation, suggesting that biliary epithelial cells are chronically exposed to these compounds in certain clinical settings. We hypothesized that biliary oxysterols resulting from liver fluke infection participate in cholangio ... | 2011 | 22044627 |
| functional analysis of the unique cytochrome p450 of the liver fluke opisthorchis felineus. | the basic metabolic cytochrome p450 (cyp) system is essential for biotransformation of sterols and xenobiotics including drugs, for synthesis and degradation of signaling molecules in all living organisms. most eukaryotes including free-living flatworms have numerous paralogues of the cyp gene encoding heme monooxygenases with specific substrate range. notably, by contrast, the parasitic flatworms have only one cyp gene. the role of this enzyme in the physiology and biochemistry of helminths is ... | 2015 | 26625139 |
| why does infection with some helminths cause cancer? | infections with opisthorchis viverrini, clonorchis sinensis and schistosoma haematobium are classified as group 1 biological carcinogens: definitive causes of cancer. these worms are metazoan eukaryotes, unlike the other group 1 carcinogens including human papilloma virus, hepatitis c virus, and helicobacter pylori. by contrast, infections with phylogenetic relatives of these helminths, also trematodes of the phylum platyhelminthes and major human pathogens, are not carcinogenic. these inconsist ... | 2015 | 26618199 |
| whole transcriptome profiling of adult and infective stages of the trematode opisthorchis felineus. | opisthorchis felineus, the trematode belonging to the family opisthorchiidae, is a causative agent of the infection called opisthorchiasis or liver fluke infection. being a close relative of opisthorchis viverrini and clonorchis sinensis (oriental liver flukes) it is encountered in northern eurasia, especially in russia, kazakhstan, belarus, ukraine, and baltic countries. whole genome data for oriental liver flukes revealed their adaptations for life in the bile duct but our knowledge of o. feli ... | 2016 | 26363139 |
| Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis. | Opisthorchis viverrini is one of the risk factors for cholangiocarcinoma (CCA) development and is endemic in Southeast Asia including Thailand. CCA is induced by chronic inflammation from a combination of mechanical damage, parasite secretions, and immunopathology. Chronic infection with O. viverrini has been associated with several hepatobiliary diseases which affect the development of hepatobiliary cancer and CCA. Therefore, reducing the pathogenesis from O. viverrini infection may be one of t ... | 2011 | 22186974 |
| schistosome and liver fluke derived catechol-estrogens and helminth associated cancers. | infection with helminth parasites remains a persistent public health problem in developing countries. three of these pathogens, the liver flukes clonorchis sinensis, opisthorchis viverrini and the blood fluke schistosoma haematobium, are of particular concern due to their classification as group 1 carcinogens: infection with these worms is carcinogenic. using liquid chromatography-mass spectrometry (lc-ms/ms) approaches, we identified steroid hormone like (e.g., oxysterol-like, catechol estrogen ... | 2014 | 25566326 |
| [the reagents kit to detect metyorchis biis, opisthorchis viverrini and clonorchis sinensis, opisthorchis felineus--agents of opisthorchiasis using technique of polymerase chain reaction in real-time]. | the helminths opisthorchis felineus, opisthorchis viverrini, clonorchis sinensis, metorchis bilis are the agents of opisthorchiasis. the actual diagnostic of parasitic diseases based on microscope analysis of samples of human feces to detect presence of ova of parasites suffers of many shortcomings, in particular low sensitivity especially at earlier stages. the purpose of this study was to compare results of detection of parasites using both classical technique and technique of specific differe ... | 2014 | 25552048 |
| infection and cancer: global distribution and burden of diseases. | infection is one of the main risk factors for cancer. | 2014 | 25512154 |
| Increased expression of TLR-2, COX-2, and SOD-2 genes in the peripheral blood leukocytes of opisthorchiasis patients induced by Opisthorchis viverrini antigen. | Re-infection with liver fluke, Opisthorchis viverrini, increases proinflammatory molecules involved in inflammation-mediated disease and carcinogenesis in an animal model. To clarify whether these genes respond to parasite antigen in peripheral blood leukocytes (PBL) of opisthorchiasis patients, we examined the transcriptional level of oxidant-generating (toll-like receptor 2 (TLR-2), nuclear factor-kappa B (NF-KB), and cyclooxygenase 2 (COX-2)), anti-oxidant-generating (manganese superoxide dis ... | 2011 | 22160279 |
| Comparative histopathology of Opisthorchis felineus and Opisthorchis viverrini in a hamster model: An implication of high pathogenicity of the European liver fluke. | European liver fluke (Opisthorchis felineus) and Asian liver fluke (Opisthorchis viverrini) are similar in morphology but comparative pathology of the infections has not been described. We therefore did comparative histopathology of both parasites in an experimental animal model. The study was conducted in 3 groups of 105 Syrian golden hamsters; the first and second groups fed with 50 metacercariae of O. felineus (OF) or O. viverrini (OV) and the last group was uninfected controls. Five hamsters ... | 2012 | 21854870 |
| first insight into cd59-like molecules of adult fasciola hepatica. | the present study focussed on investigating cd59-like molecules of fasciola hepatica. a cdna encoding a cd59-like protein (termed fhcd59-1) identified previously in the membrane fraction of the f. hepatica tegument was isolated. this homologue was shown to encode a predicted open reading frame (orf) of 122 amino acids (aa) orthologous to human cd59 with a 25 aa signal peptide, a mature protein containing 10 cysteines and a conserved cd59/ly-6 family motif "ccxxxxcn". an analysis of cdnas from tw ... | 2014 | 24955521 |
| Effects of Bayluscide on Bithynia siamensis goniomphalos, the first intermediate host of the human liver fluke, Opisthorchis viverrini, in laboratory and field trials. | The molluscicidal effects of Bayluscide (niclosamide) were investigated on Bithynia siamensis goniomphalos, the first intermediate host of human liver fluke, Opisthorchis viverrini. Lethal concentrations of 50% (LC(50)) and 95% (LC(95)) against young and adult males were 0.38 and 0.80, 0.42 and 0.86ppm, respectively. The LC(50) and LC(95) against young and adult females were 0.42 and 0.86, 0.46 and 0.97ppm, respectively. No significant differences in mortality rate between sexes or snail size (p ... | 2012 | 21864711 |
| development of a pcr assay and pyrosequencing for identification of important human fish-borne trematodes and its potential use for detection in fecal specimens. | small liver and minute intestinal flukes are highly prevalent in southeast asia. definitive diagnosis of parasite infection is usually achieved parasitologically by finding the fluke eggs in feces. however, their eggs are difficult to differentiate morphologically in fecal samples, even for experienced technicians. the present study developed a pcr assay coupled with dna pyrosequencing for identification of the fish-borne trematodes (fbt), opisthorchis viverrini, clonorchis sinensis, haplorchis ... | 2014 | 24589167 |
| molecular differentiation of opisthorchis viverrini and clonorchis sinensis eggs by multiplex real-time pcr with high resolution melting analysis. | opisthorchis viverrini and clonorchis sinensis are parasites known to be carcinogenic and causative agents of cholangiocarcinoma in asia. the standard method for diagnosis for those parasite infections is stool examination to detect parasite eggs. however, the method has low sensitivity, and eggs of o. viverrini and c. sinensis are difficult to distinguish from each other and from those of some other trematodes. here, we report a multiplex real-time pcr coupled with high resolution melting (hrm) ... | 2013 | 24516275 |
| rapid detection of opisthorchis viverrini copro-dna using loop-mediated isothermal amplification (lamp). | opisthorchis viverrini and other foodborne trematode infections are major health problem in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. we therefore established a rapid, sensitive and specific method for detecting o. viverrini infection from the stool samples using the loop-mediated isothermal ... | 2012 | 21871581 |
| risk factors for cholangiocarcinoma in high-risk area of thailand: role of lifestyle, diet and methylenetetrahydrofolate reductase polymorphisms. | background and aim: cholangiocarcinoma (cca) is the most common cancer in northeast thailand. endemicity of opisthorchis viverrini (ov) - a known carcinogen - is responsible, but although infection is very common, the lifetime risk of cca is only 5%. other co-factors must exist, including aspects of lifestyle or diet along with variations in genetic susceptibility to them. change in methylenetetrahydrofolate reductase (mthfr) activity may influence both dna methylation and synthesis. this study ... | 2011 | 22189445 |
| Fecal bacterial contamination in natural water reservoirs as an indicator of seasonal infection by Opisthorchis viverrini in snail intermediate hosts. | Opisthorchis viverrini, a carcinogenic liver fluke, requires Bithynia snails as the first intermediate host, which release cercariae after ingesting fluke eggs from contaminated water. Fecal bacterial contamination and O. viverrini-infected Bithynia snails were investigated in samples collected from natural water reservoirs in Ban Phai, Chonnabot and Muang Districts (Ban Lerngpeuy) in Khon Kaen Province, northeast Thailand, where there is a high incidence of cholangiocarcinoma. Water was sampled ... | 2012 | 21871971 |
| [strengthening the research on clonorchiasis in china]. | liver flukes mainly include clonorchis sinensis, opisthorchis viverrini, and opisthorchis felineus. the international congress of liver flukes was held in khon kaen, thailand, during 7-8th march, 2011. the congress assembled a wide array of studies and reflected the current status of research, control and prevention of liver flukes in the world. this paper summarizes basic information from the meeting. meanwhile, based on the research status and needs for control and prevention, priorities of re ... | 2011 | 21970113 |
| fishborne trematode metacercariae in luang prabang, khammouane, and saravane province, lao pdr. | fishborne trematode (fbt) metacercariae were investigated in fish from 3 provinces of lao pdr. total 242 freshwater fish of 40 species were collected in local markets of luang prabang (59 fish of 16 species), khammouane (81 fish of 19 species), and saravane (97 fish of 14 species), and each of them was examined by artificial digestion method. four species of metacercariae (opisthorchis viverrini, haplorchis taichui, haplorchis yokogawai, and centrocestus formosanus) were detected. o. viverrini w ... | 2013 | 23467784 |
| protective immunization of hamsters against opisthorchis viverrini infection is associated with the reduction of tgf-β expression. | opisthorchis viverrini infection is a significant health problem in thailand and other countries in southeast asia. there is little known about the mechanisms of the immune response to o. viverrini in immunoprotection. however, it has been reported that this parasite can suppress both cell and antibody mediated immune responses. the tgf-β and il-10 are immunosuppressive cytokines that play an important role in inhibition of host immune response leading to worm survival. in this study, we immuniz ... | 2012 | 22266215 |
| microproteinuria during opisthorchis viverrini infection: a biomarker for advanced renal and hepatobiliary pathologies from chronic opisthorchiasis. | approximately 680 million people are at risk of infection with opisthorchis viverrini (ov) and clonorchis sinensis, with an estimated 10 million infected with ov in southeast asia alone. while opisthorchiasis is associated with hepatobiliary pathologies, such as advanced periductal fibrosis (apf) and cholangiocarcinoma (cca), animal models of ov infection show that immune-complex glomerulonephritis is an important renal pathology that develops simultaneously with hepatobiliary pathologies. a car ... | 2013 | 23717698 |
| rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples using a duplex real-time fluorescence resonance energy transfer pcr and melting curve analysis. | we developed a single step duplex real-time fluorescence resonance energy transfer (fret) pcr merged with melting curve analysis for the fast detection and differentiation of clonorchis sinensis and opisthorchis viverrini eggs in human fecal samples. two species of mitochondrial nadh dehydrogenase subunit 2 (nad2) dna elements, the 165-bp nad2 product of c. sinensis and the 209-bp nad2 product of o. viverrini, were amplified by species-specific primers, and the fluorescence melting curve analyse ... | 2012 | 22246366 |
| mechanisms of oxysterol-induced disease: insights from the biliary system. | oxysterols are oxidized species of cholesterol that are derived from exogenous (e.g. dietary) and endogenous (in vivo) sources. oxysterols play critical roles in normal physiologic functions as well as in pathophysiologic processes in a variety of organ systems. this review provides an overview of oxysterol biology from the vantage point of the biliary system. several oxysterols have been identified in human bile in the context of biliary tract infection and inflammation. this finding has led to ... | 2012 | 23630545 |
| the tumorigenic liver fluke opisthorchis viverrini--multiple pathways to cancer. | liver fluke infection caused by opisthorchis viverrini is a major public health problem in thailand and adjacent countries. in addition to infection-associated morbidity, infection with o. viverrini and the related clonorchis sinensis are unarguable risk factors for cholangiocarcinoma (caa, bile-duct cancer). here we review the pathogenesis of opisthorchiasis and the association between o. viverrini infection and bile-duct cancer, focusing on the molecular parallels between wound healing, chroni ... | 2012 | 22947297 |
| recurrent emergence of structural variants of ltr retrotransposon csrn1 evolving novel expression strategy and their selective expansion in a carcinogenic liver fluke, clonorchis sinensis. | autonomous retrotransposons, in which replication and transcription are coupled, encode the essential gag and pol genes as a fusion or separate overlapping form(s) that are expressed in single transcripts regulated by a common upstream promoter. the element-specific expression strategies have driven development of relevant translational recoding mechanisms including ribosomal frameshifting to satisfy the protein stoichiometry critical for the assembly of infectious virus-like particles. retrotra ... | 2017 | 28322871 |
| insights into scp/taps proteins of liver flukes based on large-scale bioinformatic analyses of sequence datasets. | scp/taps proteins of parasitic helminths have been proposed to play key roles in fundamental biological processes linked to the invasion of and establishment in their mammalian host animals, such as the transition from free-living to parasitic stages and the modulation of host immune responses. despite the evidence that scp/taps proteins of parasitic nematodes are involved in host-parasite interactions, there is a paucity of information on this protein family for parasitic trematodes of socio-ec ... | 2012 | 22384000 |
| parasites and malignancies, a review, with emphasis on digestive cancer induced by cryptosporidium parvum (alveolata: apicomplexa). | the international agency for research on cancer (iarc) identifies ten infectious agents (viruses, bacteria, parasites) able to induce cancer disease in humans. among parasites, a carcinogenic role is currently recognized to the digenetic trematodes schistosoma haematobium, leading to bladder cancer, and to clonorchis sinensis or opisthorchis viverrini, which cause cholangiocarcinoma. furthermore, several reports suspected the potential association of other parasitic infections (due to protozoan ... | 2012 | 22348213 |
| [control of opisthorchis viverrini infection for cholangiocarcinoma prevention]. | the international agency for research on cancer (iarc) has classified two liver flukes as carcinogenic to humans (group 1): opisthorchis viverrini in 1994 and clonorchis sinensis in 2009. this review is focused on o. viverrini, the most studied of these two trematodes, which infects nearly 10 million people in southeast asia. the life cycle involves two intermediate hosts living in fresh water: a snail of the genus bithynia and a ciprinid fish. the definitive hosts (human, cat, dog) become infec ... | 2017 | 28105582 |
| effects of melatonin on oxidative stress, and resistance to bacterial, parasitic, and viral infections: a review. | melatonin, a hormone secreted by the pineal gland, works directly and indirectly as a free radical scavenger. its other physiological or pharmacological activities could be dependent or independent of receptors located in different cells, organs, and tissues. in addition to its role in promoting sleep and circadian rhythms regulation, it has important immunomodulatory, antioxidant, and neuroprotective effects suggesting that this indole must be considered as a therapeutic alternative against inf ... | 2014 | 24811367 |
| detection of the carcinogenic liver fluke opisthorchis viverrini using a mini parasep sf faecal parasite concentrator. | the mini parasep sf fecal parasite concentrator (mpsfc) is a new modification of the closed concentration system, which can easily be adopted in any routine clinical pathology laboratory. here we describe our experience with the system in diagnosing opisthorchis viverrini. a total of 199 fecal samples was submitted for routine examination in the clinical pathology laboratory of suranaree university of technology hospital, nakhon ratchasima province, thailand, during august to october 2015. out o ... | 2016 | 26838241 |
| liver fluke infection and cholangiocarcinoma: a review. | parasites are significant groups for carcinogenesis among which liver flukes, including opisthorchis viverrini and clonorchis sinensis, are typical representatives causing cholangiocarcinoma (cca), the second most common primary hepatic malignancy with dismal prognosis. o. viverrini is prevalent in southeast asia, infecting 10 million people while c. sinensis has a wider distribution in east asia and several southeast asian countries, affecting more than 35 million people's health. these two wor ... | 2017 | 27718017 |
| high prevalence of haplorchiasis in nan and lampang provinces, thailand, proven by adult worm recovery from suspected opisthorchiasis cases. | opisthorchiasis, a risk factor for cholangiocarcinoma in humans, is of public health importance in thailand. the annual surveillance reports from nan and lampang provinces, thailand, for the year 2011 showed an opisthorchiasis prevalence of over 70% by recovery of eggs in the feces. this study investigated whether most cases are actually due to minute intestinal flukes (mif) rather than opisthorchis viverrini, as the eggs of both can hardly be differentiated by morphology. fifty and 100 cases fr ... | 2013 | 24516289 |
| identification and characterization of protein 14-3-3 in carcinogenic liver fluke opisthorchis viverrini. | protein 14-3-3s are abundant phospho-serine/threonine binding proteins, which are highly conserved among eukaryotes. members of this protein family mediate metabolism and signal transduction networks through binding to hundreds of other protein partners. protein 14-3-3s have been studied in other species of parasitic helminthes, but little is known about this protein in the carcinogenic liver fluke opisthorchis viverrini. in this study, we identified and characterized protein 14-3-3s of o. viver ... | 2016 | 27989833 |
| chronic opisthorchis viverrini infection changes the liver microbiome and promotes helicobacter growth. | adults of opisthorchis viverrini reside in the biliary system, inducing inflammation of bile ducts and cholangitis, leading to hepatobiliary disease (hbd) including cholangiocarcinoma. o. viverrini infection also has major implications for the bacterial community in bile ducts and liver. to investigate this in chronic o. viverrini infection (≥ 8 months p.i.), bacterial genomic dna from livers of hamsters and from worms was investigated using culture techniques, pcr for helicobacter spp. and high ... | 2016 | 27806126 |
| immunodiagnosis of opisthorchiasis using parasite cathepsin f. | opisthorchis viverrini, a food-borne trematode parasite endemic in the lower mekong countries, is conventionally diagnosed by stool examination. however, parasitological stool-based diagnosis can be unreliable in light infections. the goal of this study was to develop the immunodiagnosis of opisthorchiasis using cathepsin f cysteine protease of o. viverrini in both indirect and sandwich elisa assays. a recombinant o. viverrini cathepsin f (rov-cf) of 40 kda was expressed in e. coli strain bl21 ( ... | 2015 | 26344868 |
| chromosome morphometry in opisthorchiid species (platyhelminthes, trematoda). | few existing studies have dealt with cytogenetics in trematodes, largely due to the attendant technical difficulty of chromosome preparation. we performed a comparative analysis of chromosomes in five opistorchiid species, including opisthorchis felineus rivolta, 1884, opisthorchis viverrini poirier, 1886, clonorchis sinensis cobbold, 1875, metorchis xanthosomus creplin 1846, and metorchis bilis (braun, 1790) odening, 1962. for some of these species, no detailed morphometric description of their ... | 2016 | 27404484 |
| characterization and functional analysis of fatty acid binding protein from the carcinogenic liver fluke, opisthorchis viverrini. | in the present study, the cdna encoding fabp (ov-fabp) was isolated from the adult stage of opisthorchis viverrini and characterized. the ov-fabp protein sequence (107 amino acids) was predicted to have a molecular mass of 12.26kda and an isoelectric point (pi) of 6.82. this sequence had a high identity and similarity to cs-fabp of the related opisthorchid clonorchis sinensis. multiple sequence alignment with fabps from other parasitic flatworms and mammals showed a number of conserved amino aci ... | 2016 | 27143603 |
| opisthorchis viverrini: analysis of the sperm-specific rhophilin associated tail protein 1-like. | concurrent deficiency of rhophilin associated tail protein (ropn1) and ropn1-like (ropn1l) in mice causes structural abnormalities and immotility of sperm and thereby infertility. in the present research, ropn1l of the human liver fluke opisthorchis viverrini was molecularly characterized and showed unexpected potential as a diagnostic tool. ropn1l transcripts were detected in 2-week-old juveniles by rt-pcr. immunohistochemical analysis of the adult worm localized the protein in testis lobes, se ... | 2014 | 25124046 |
| prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand. | to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region. | 2014 | 24507641 |
| diagnosis of fasciola gigantica infection using a monoclonal antibody-based sandwich elisa for detection of circulating cathepsin b3 protease. | a reliable monoclonal antibody (moab)-based sandwich enzyme-linked immunosorbent assay (sandwich elisa) was developed for the detection of circulating cathepsin b3 protease (catb3) in the sera from mice experimentally infected with fasciola gigantica and cattle naturally infected with the same parasite. the moab 2f9 and biotinylated rabbit polyclonal anti-recombinant catb3 antibody were selected due to their high reactivities and specificities to f. gigantica catb3 antigen based on indirect elis ... | 2013 | 23567552 |
| helicobacter species are possible risk factors of cholangiocarcinoma. | several infectious agents are considered to be causes of cancer in human, mainly hepatitis b and c viruses, high-risk human pailloma viruses, helicobacter pylori, clonorchis sinensis, and opisthorchis viverrini. here we described the evident research and the association between helicobacter spp. and biliary tract cancer particularly cholangiocarcinoma (cca). global epidemiological studies have suggested that helicobacter spp. are possible risk factors for biliary tract diseases. molecular studie ... | 2016 | 26838240 |
| experimental and modelling investigations of opisthorchis viverrini miracidia transmission over time and across temperatures: implications for control. | transmissibility is a significant factor in parasite fitness. the rate and magnitude of parasite transmission affect prevalence and infection intensity in individual hosts and are influenced by environmental factors. in this context, the objectives of this study were: (i) to experimentally assess opisthorchis viverrini miracidia survival and infectivity over time and across temperatures; and (ii) to combine these experimental results with environmental data to build a key component of a transmis ... | 2017 | 28237890 |
| hemozoin is a product of heme detoxification in the gut of the most medically important species of the family opisthorchiidae. | many species of trematodes such as schistosoma spp., fasciola hepatica and echinostoma trivolvis are blood-feeding parasites. nevertheless, there is no consensus on the feeding habits of the family opisthorchiidae (opisthorchis felineus, opisthorchis viverrini and clonorchis sinensis). previously, histological studies of o. felineus and c. sinensis revealed some dark stained material in their gut lumen. in this study we conducted a comprehensive analysis of the gut contents of three members of t ... | 2016 | 26812025 |
| role of socio-cultural and economic factors in cyprinid fish distribution networks and consumption in lawa lake region, northeast thailand: novel perspectives on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is a fish-borne parasite endemic in parts of lao pdr, cambodia, southern vietnam and northeast thailand (isaan) where an estimated 10 million people are infected. human ov infection, associated with hepatobiliary complications, including cholangiocarcinoma (cca), occurs when infected fish are consumed raw or undercooked, a longstanding cultural tradition in the region. this mixed- methods descriptive study was carried out in isaan villages around lawa lake, khon kaen ... | 2017 | 28216369 |
| the complete mitochondrial genome of metorchis orientalis (trematoda: opisthorchiidae): comparison with other closely related species and phylogenetic implications. | metorchis orientalis (trematoda: opisthorchiidae) is an important trematode infecting many animals and humans, causing metorchiasis. in the present study, the complete mitochondrial (mt) genome of m. orientalis was sequenced. the complete mt genome of m. orientalis is 13,834 bp circular dna molecule and contains 12 protein-coding genes, 22 transfer rna genes, and two ribosomal rna genes. the gene content and arrangement of m. orientalis is the same as those of opisthorchiidae trematodes (opistho ... | 2016 | 26805437 |
| phylogenetic characterization of clonorchis sinensis proteins homologous to the sigma-class glutathione transferase and their differential expression profiles. | glutathione transferase (gst) is one of the major antioxidant proteins with diverse supplemental activities including peroxidase, isomerase, and thiol transferase. gsts are classified into multiple classes on the basis of their primary structures and substrate/inhibitor specificity. however, the evolutionary routes and physiological environments specific to each of the closely related bioactive enzymes remain elusive. the sigma-like gsts exhibit amino acid conservation patterns similar to the pr ... | 2016 | 26792248 |
| application of environmental dna analysis for the detection of opisthorchis viverrini dna in water samples. | opisthorchiasis, which can lead to cholangiocarcinoma in cases of chronic infection, is a major public health problem in southeast asian countries. the trematode, opisthorchis viverrini, is the causative agent of the disease. accurate and rapid monitoring of o. viverrini is crucial for disease prevention and containment. therefore, in this study we sought to develop a novel species-specific real-time pcr assay for detecting o. viverrini using environmental dna (edna). the diagnostic sensitivity ... | 2017 | 28108370 |
| ultrasonographic features of hepatobiliary pathology in opisthorchiasis and opisthorchiasis-associated cholangiocarcinoma. | infection with the liver fluke, opisthorchis viverrini (ov), is an important public health problem in northeast thailand where people have a habit of eating raw or inadequately cooked fish. opisthorchiasis is associated with a number of asymptomatic hepatobiliary abnormalities and cholangiocarcinoma (cca) that can be observed conveniently by ultrasonography. based on our accumulated data, characteristic features of ultrasonographic findings of hepatobiliary pathology in ov infection are reviewed ... | 2016 | 27956092 |
| clonorchis sinensis and opisthorchis spp. in vietnam: current status and prospects. | clonorchis sinensis and opisthorchis viverrini are clinically important small liver flukes because of their known association with development of cholangiocarcinoma. in vietnam, high prevalence of c. sinensis infection in humans was previously reported in northern provinces, and o. viverrini infection has been detected in several central provinces. however, diagnosis of c. sinensis and o. viverrini infections in the past was merely based on faecal egg examination. this method alone can lead to m ... | 2016 | 26740358 |
| genome-wide characterization of microsatellites and marker development in the carcinogenic liver fluke clonorchis sinensis. | clonorchis sinensis is an important carcinogenic human liver fluke endemic in east and southeast asia. there are several conventional molecular markers that have been used for identification and genetic diversity; however, no information about microsatellites of this liver fluke is published so far. we here report microsatellite characterization and marker development for a genetic diversity study in c. sinensis, using a genome-wide bioinformatics approach. based on our search criteria, a total ... | 2015 | 25782682 |
| rapid detection and differentiation of clonorchis sinensis and opisthorchis viverrini using real-time pcr and high resolution melting analysis. | clonorchis sinensis and opisthorchis viverrini are both important fish-borne pathogens, causing serious public health problem in asia. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis for the specific detection and rapid identification of c. sinensis and o. viverrini. primers targeting cox1 gene were highly specific for these liver flukes, as evidenced by the negative amplification of closely related trematodes. assays using genomic dna ex ... | 2014 | 25386621 |
| specific diagnosis of opisthorchis viverrini using loop-mediated isothermal amplification (lamp) targeting parasite microsatellites. | opisthorchis viverrini and other food-borne trematode infections are major health problems in thailand, the lao people's democratic republic, vietnam and cambodia. differential diagnosis of o. viverrini based on the microscopic observation of parasite eggs is difficult in areas where clonorchis sinensis and minute intestinal flukes coexist. recently, loop-mediated isothermal amplification (lamp) has been widely used for detection and identification of trematode for its simple method that is usef ... | 2015 | 25268466 |
| hepatitis b virus infection and intrahepatic cholangiocarcinoma. | intrahepatic cholangiocarcinoma (icc) is a devastating malignant tumor arising from the peripheral intrahepatic bile duct epithelium. the incidence and mortality of icc is markedly increasing over the past two decades worldwide, though the cause for this rise in incidence is unclear, thus intensifying the search for alternative etiological agents and pathogenetic mechanisms. hepatolithiasis, primary sclerosing cholangitis, parasitic infection (opisthorchis viverrini or clonorchis sinensis), fibr ... | 2014 | 24914333 |
| roles of liver fluke infection as risk factor for cholangiocarcinoma. | several factors are known to be associated with risk of cholangiocarcinoma (cca) and infection with the liver flukes, opisthorchis viverrini and clonorchis sinensis, has often been singled out as the leading risk factor in east and southeast asia. in this review, current knowledge of their biology, life cycle, and pathogenesis of o. viverrini, and its role as a carcinogenic parasite are presented. the trends of age-specific incidence of liver cancer in khon kaen, northeast thailand are considere ... | 2014 | 24408775 |
| retrotransposon ov-rte-1 from the carcinogenic liver fluke opisthorchis viverrini: potential target for dna-based diagnosis. | infections by the fish-borne liver flukes opisthorchis viverrini and clonorchis sinensis can lead to bile duct cancer. these neglected tropical disease pathogens occur in east asia, with o. viverrini primarily in thailand and laos and c. sinensis in cambodia, vietnam, and china. genomic information about these pathogens holds the potential to improve disease treatment and control. transcriptome analysis indicates that mobile genetic elements are active in o. viverrini, including a novel non-long ... | 2014 | 24394447 |
| cpg oligodeoxynucleotides with crude parasite antigens reduce worm recovery in opisthorchis viverrini infected hamsters. | opisthorchis viverrini, a human liver fluke, is still an endemic parasitic infection in thailand and nearly all countries in southeast asia. o. viverrini induces a chronic stage of infection in hamsters. during the first 2 weeks of infection, th1 inducing cytokine, il-12, increased but was down regulated in chronic infection. in this study it was found that unmethylated-cpg odn (oligodeoxynucleotides) 1826 increased hamster mononuclear cell proliferation and stimulated ifn-γ production in vitro. ... | 2016 | 27737764 |
| global status of fish-borne zoonotic trematodiasis in humans. | fishborne zoonotic trematodes (fzt), infecting humans and mammals worldwide, are reviewed and options for control discussed. fifty nine species belonging to 4 families, i.e. opisthorchiidae (12 species), echinostomatidae (10 species), heterophyidae (36 species) and nanophyetidae (1 species) are listed. some trematodes, which are highly pathogenic for humans such as clonorchis sinensis, opisthorchis viverrini, o. felineus are discussed in detail, i.e. infection status in humans in endemic areas, ... | 2013 | 23990419 |
| centrocestus formosanus (heterophyidae): human infections and the infection source in lao pdr. | in lao people's democratic republic (pdr), we detected 7 patients infected with centrocestus formosanus (1-122 adult specimens) after praziquantel treatment and purgation, together with several other trematode species including opisthorchis viverrini and haplorchis taichui. the patients were all men, 23-42 yr-of-age. three subjects were from vientiane municipality and 1 each were from khammouane, saravane, champassak, and xiengkhouang province. the patients had frequently eaten raw freshwater fi ... | 2013 | 23116489 |
| modifying health behavior for liver fluke and cholangiocarcinoma prevention with the health belief model and social support theory. | the liver fluke opisthorchis viverrini is a serious health problem in thailand. infection is associated with cholangiocarcinoma (cca), endemic among human populations in northeast and north thailand where raw fish containing fluke metacercariae are frequently consumed. recently, thailand public health authorities have been organized to reduce morbidity and mortality particularly in the northeast through o. viverrini and cca screening projects. health modfication is one of activities included in ... | 2016 | 27644606 |
| fish sharing as a risk factor for opisthorchis viverrini infection: evidence from two villages in north-eastern thailand. | foodborne trematodiasis (fbt) is a significant global health problem, with the liver flukes opisthorchis viverrini, o. felineus, and clonorchis sinensis contributing to half of the global burden of fbt. north-eastern thailand where o. viverrini is endemic and un-cooked fish dishes remain an integral part of the food culture has the highest reported incidence of opisthorchiasis, including associated cholangiocarcinoma. both food sharing and eating practices are potentially important factors in ft ... | 2017 | 28372560 |
| population pharmacokinetic modeling of tribendimidine metabolites in opisthorchis viverrini-infected adults. | there is a pressing need for alternative treatments against the liver fluke opisthorchis viverrini oral tribendimidine is a promising candidate, but its population pharmacokinetic properties are unknown. two phase iia trials were conducted in laos in o. viverrini-infected adults receiving single oral doses of 25 to 600 mg tribendimidine administered as different formulations in each study (study 1 used 200-mg tablets, and study 2 used 50-mg tablets). venous whole blood, plasma, and capillary dri ... | 2016 | 27431233 |
| development of mitochondrial loop-mediated isothermal amplification for detection of the small liver fluke opisthorchis viverrini (opisthorchiidae; trematoda; platyhelminthes). | mitochondrial dna sequences offer major advantages over the more usual nuclear targets for loop-mediated isothermal amplification approaches (mito-lamp) because multiple copies occur in every cell. four lamp primers [f3, fip(f1c+f2), bip(b1c+b2), and b3] were designed based on the mitochondrial nad1 sequence of opisthorchis viverrini and used for a highly specific assay (mito-ovlamp) to distinguish dna of o. viverrini from that of another opisthorchiid (clonorchis sinensis) and other trematodes ... | 2012 | 22322346 |
| prevalence of haplorchis taichui among humans and fish in luang prabang province, lao pdr. | this study confirmed the prevalence of the intestinal fluke haplorchis taichui (trematoda: heterophyidae) among people and fish in luang prabang province, lao pdr. fecal specimens were collected from 559 riparian people (229 males and 330 females), residing in 4 districts (luang prabang, xieng ngeun, pak ou, and nam bak) and were examined by the kato-katz fecal smear technique. the overall helminth egg positive rate was 64.9%. the positive rate for small trematode eggs (ste), which may include h ... | 2014 | 24754919 |
| protection against fasciola gigantica infection in mice by vaccination with recombinant juvenile-specific cathepsin l. | fasciola gigantica cathepsin l1h (fgcatl1h) is one of the major cathepsin l released by juveniles of f. gigantica to aid in the invasion of host's tissues. due to its high sequence similarity with other cathepsin l (catl) isoforms of late stage f. gigantica, it was considered to be a good vaccine candidate that can block all catl-mediated protease activities and affect juveniles as well as adult parasites. in this study, recombinant profgcatl1h protein expressed in yeast, pichia pastoris, system ... | 2015 | 25701317 |
| assessing the role of landscape connectivity on opisthorchis viverrini transmission dynamics. | opisthorchis viverrini (ov) is one of the most important human parasitic diseases in southeast asia. although the concept of connectivity is widely used to comprehend disease dispersal, knowledge of the influences of landscape connectivity on ov transmission is still rudimentary. this study aimed to investigate the role of landscape connectivity in ov transmission between the human and the first intermediate snail hosts. fieldwork was conducted in three villages respectively in kamalasai distric ... | 2016 | 27265875 |
| production and characterization of a monoclonal antibody against recombinant saposin-like protein 2 of fasciola gigantica. | a monoclonal antibody (moab) against recombinant fasciola gigantica saposin-like protein 2 (rfgsap-2) was produced by hybridoma technique using spleen cells from balb/c mice immunized with rfgsap-2. this moab is an igg1, κ light chain isotype. by immunoblotting and indirect elisa, the moab reacted specifically with rfgsap-2, the natural fgsap-2 at 10kda in whole body (wb) and excretory-secretory (es) fractions of f. gigantica. it did not cross react with antigens in wb fractions from other paras ... | 2013 | 23164839 |
| delineating distinct heme-scavenging and binding functions of domains in mf6p/hdm proteins from parasitic flatworms. | mf6p/fhhdm-1 is a small protein secreted by the parasitic flatworm (trematode) fasciola hepatica and belonging to a broad family of heme-binding proteins (mf6p/hdms). mf6p/hdms are of interest for understanding heme homeostasis in trematodes and also as potential targets for the development of new flukicides. moreover, interest in these molecules has also increased due to their immunomodulatory properties. here we have extended our previous findings on the mechanism of mf6p/hdm-heme interactions ... | 2017 | 28348084 |
| differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma. | parts of southeast asia have the highest incidence of intrahepatic cholangiocarcinoma (cca) in the world due to infection by the liver fluke opisthorchis viverrini (ov). ov-associated cca is the culmination of chronic ov-infection, with the persistent production of the growth factors and cytokines associated with persistent inflammation, which can endure for years in ov-infected individuals prior to transitioning to cca. isobaric labelling and tandem mass spectrometry of liver tissue from a hams ... | 2017 | 28232516 |
| wound healing and cancer progression in opisthorchis viverrini associated cholangiocarcinoma. | infection with the human liver fluke opisthorchis viverrini induces cancer of the bile ducts, cholangiocarcinoma (cca). it was shown previously that o. viverrini-secreted proteins accelerate wound resolution in human cholangiocytes. recombinant ov-grn-1 (o. viverrini-derived gene encoding granulin-like growth factor) induced angiogenesis and accelerated mouse wound healing. given the striking similarities of wound healing and cancer progression, here we discuss the major implications of this fin ... | 2016 | 27130317 |