| comparative proteomic analysis of aureobasidium pullulans in the presence of high and low levels of nitrogen source. | pullulan, produced by aureobasidium pullulans strain, has been broadly used in the food and medical industries. however, relatively little is known concerning the molecular basis of pullulan biosynthesis of this strain. in this paper, the effect of different concentrations of (nh4)2so4 on pullulan fermentation was studied. proteomics containing two-dimensional gel electrophoresis (2-de) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight mass spectrometry (maldi-tof/tof ... | 2014 | 25290967 |
| efficient pullulan production by bioconversion using aureobasidium pullulans as the whole-cell catalyst. | in this study, pullulan production was achieved by whole-cell bioconversion with aureobasidium pullulans cctcc m 2012259. response surface methodology was applied to optimize the seed medium for incubating cells with high capability of pullulan bioconversion. three medium components, namely, yeast extract, mgso4·7h2o, and glucose were identified by plackett-berman design as significant factors affecting the cells' pullulan bioconversion capability. a three-level box-behnken design was then emplo ... | 2015 | 25277414 |
| effect of meadowsweet flower extract-pullulan coatings on rhizopus rot development and postharvest quality of cold-stored red peppers. | the study involved an examination of the antifungal activity on red peppers of pullulan coating (p) and pullulan coating containing either water-ethanol (p + eemf) or ethanol extract of meadowsweet flowers (p + eemf). pullulan was obtained from a culture of aureobasidium pullulans b-1 mutant. both non-inoculated peppers and those artificially inoculated with rhizopus arrhizus were coated and incubated at 24 °c for 5 days. the intensity of the decay caused by rhizopus arrhizus in the peppers with ... | 2014 | 25157463 |
| two cases of multiple subcutaneous cystic phaeohyphomycosis in immunocompromised patients with a rare causative organism. | phaeohyphomycosis comprises a spectrum of mycotic infectious diseases caused by heterogeneous group of phaeoid fungi. a subcutaneous cystic swelling with histological hallmark of pigmented hyphae in tissues accompanied by pyogenic granuloma is diagnostic of phaeohyphomycosis. we present two cases of phaeohyphomycosis in 65- and 55-year-old male agriculturalists, who presented with multiple, cystic soft-tissue masses involving extremities. culture was done in one case and the specific organism wa ... | 2014 | 25071277 |
| characterization of a highly thermostable glycoside hydrolase family 10 xylanase from malbranchea cinnamomea. | a thermostable xylanase (mcxyn10) from the thermophilic fungus malbranchea cinnamomea strain s168 was purified and biochemically characterized. the enzyme was purified to homogeneity with a molecular mass of 43.5 kda on sds-page. the optimal ph and temperature of the purified enzyme were ph 6.5 and 80°c, respectively. the enzyme showed a broad range of ph stability (ph 4.0-10.5), and was stable up to 70°c with a thermal denaturing half life of 76.0 min. the enzyme exhibited strict specificity fo ... | 2014 | 25058752 |
| yeasts found on an ephemeral reproductive caste of the leaf-cutting ant atta sexdens rubropilosa. | winged males of leaf-cutting ants are considered an ephemeral reproductive caste only produced before the mating flight season. although much is known about the yeast diversity found in fungus gardens of attine ants, no study has focused on the yeasts associated with males of leaf-cutting ants. here, we surveyed the yeasts on the integuments of males of atta sexdens rubropilosa and assessed their potential role in the attine ant-microbe symbiosis. using culture-dependent techniques, we found yea ... | 2014 | 25012689 |
| in vitro activities of eight antifungal drugs against 104 environmental and clinical isolates of aureobasidium pullulans. | aureobasidium pullulans is an unusual agent of phaeohyphomycosis. the in vitro activities of antifungals against 104 isolates of aureobasidium pullulans var. pullulans and a. pullulans var. melanigenum revealed low mic90s of amphotericin b, posaconazole, and itraconazole. however, they were resistant to fluconazole (≥64 μg/ml) and had high mics of voriconazole, isavuconazole, caspofungin, and micafungin. | 2014 | 25001309 |
| genome sequencing of four aureobasidium pullulans varieties: biotechnological potential, stress tolerance, and description of new species. | aureobasidium pullulans is a black-yeast-like fungus used for production of the polysaccharide pullulan and the antimycotic aureobasidin a, and as a biocontrol agent in agriculture. it can cause opportunistic human infections, and it inhabits various extreme environments. to promote the understanding of these traits, we performed de-novo genome sequencing of the four varieties of a. pullulans. | 2014 | 24984952 |
| inhibition of uvb-induced skin damage by exopolymers from aureobasidium pullulans sm-2001 in hairless mice. | because antioxidants from natural sources may be an effective approach to the treatment and prevention of uv radiation-induced skin damage, the effects of purified exopolymers from aureobasidium pullulans sm-2001 ('e-ap-sm2001') were evaluated in uvb-induced hairless mice. e-ap-sm2001 consists of 1.7% β-1,3/1,6-glucan, fibrous polysaccharides and other organic materials, such as amino acids, and mono- and di-unsaturated fatty acids (linoleic and linolenic acids) and shows anti-osteoporotic and i ... | 2015 | 24964914 |
| inactivation of bacteria and yeast using high-frequency ultrasound treatment. | high-frequency (850 khz) ultrasound was used to inactivate bacteria and yeast at different growth phases under controlled temperature conditions. three species of bacteria, enterobacter aerogenes, bacillus subtilis and staphylococcus epidermidis as well as a yeast, aureobasidium pullulans were considered. the study shows that high-frequency ultrasound is highly efficient in inactivating the bacteria in both their exponential and stationary growth phases, and inactivation rates of more than 99% w ... | 2014 | 24835956 |
| efficient production of pullulan using rice hull hydrolysate by adaptive laboratory evolution of aureobasidium pullulans. | pullulan production by aureobasidium pullulans cctcc m 2012259 using rice hull hydrolysate as the carbon source was conducted. the acetic acid in the hydrolysate was demonstrated to exert a negative effect on pullulan biosynthesis. instead of employing expensive methods to remove acetic acid from the hydrolysate, a mutant a. pullulans arh-1 was isolated following 20 cycles of adaptive laboratory evolution of the parental strain on medium containing acetic acid. the maximum pullulan production ac ... | 2014 | 24835913 |
| novel symbiotic protoplasts formed by endophytic fungi explain their hidden existence, lifestyle switching, and diversity within the plant kingdom. | diverse fungi live all or part of their life cycle inside plants as asymptomatic endophytes. while endophytic fungi are increasingly recognized as significant components of plant fitness, it is unclear how they interact with plant cells; why they occur throughout the fungal kingdom; and why they are associated with most fungal lifestyles. here we evaluate the diversity of endophytic fungi that are able to form novel protoplasts called mycosomes. we found that mycosomes cultured from plants and p ... | 2014 | 24777121 |
| cofactor and co2 donor regulation involved in reductive routes for polymalic acid production by aureobasidium pullulans cctcc m2012223. | polymalic acid (pma) is a water-soluble polyester with many attractive properties for biomedical application. its monomer l-malic acid is widely used in the food industry and also a potential c4 platform chemical. cofactor and co2 donor involved in the reductive routes were investigated for pma production by aureobasidium pullulans. biotin as the key cofactor of pyruvate carboxylase was favor for the pma biosynthesis. na2co3 as co2 donor can obviously improved pma titer when compared with no co2 ... | 2014 | 24700133 |
| aureobasidium pullulans as a source of liamocins (heavy oils) with anticancer activity. | liamocins are structurally unique, heavier-than-water “oils” produced by certain strains of aureobasidium pullulans. the aim of the current study is to identify new sources of liamocins and evaluate their potential as anticancer agents. nine strains of a. pullulans from phylogenetic clades 8, 9, and 11 were examined for the first time for production of liamocins. strains in these clades have only been isolated from tropical environments, and all strains tested here were from various locations in ... | 2014 | 24659335 |
| biosynthesis and hyper production of pullulan by a newly isolated strain of aspergillus japonicus-vit-sb1. | the main focus of this study was to screen and characterize novel microbial strains isolated from culinary leaf samples, capable of producing high concentrations of pullulan. hundred isolates were screened from the phylloplane of different plants. the results revealed that eight strains had the capability to produce exopolysaccharide (eps) and only one potential strain (designated as vit-sb1) could produce the significant amount of eps (3.9 ± 0.02%) on the 6th day of the fermentation without opt ... | 2014 | 24609496 |
| high-level pullulan production by aureobasidium pullulans var. melanogenium p16 isolated from mangrove system. | after over 100 strains of aureobasidium spp. isolated from mangrove system were screened for their ability to produce exopolysaccharide (eps), it was found that aureobasidium pullulans var. melanogenium p16 strain among them could produce high level of eps. under the optimal conditions, 65.3 g/l eps was produced by the p16 strain within 120 h at flask level. during 10-l batch fermentation, when the medium contained 120.0 g/l sucrose, 67.4 g/l of eps and 23.1 g/l of cell dry weight in the culture ... | 2014 | 24604498 |
| biological activities of organic extracts of four aureobasidium pullulans varieties isolated from extreme marine and terrestrial habitats. | we report on the screening for biological activities of organic extracts from seven strains that represent four varieties of the fungus aureobasidium pullulans, that is a. pullulans var. melanogenum, a. pullulans var. pullulans, a. pullulans var. subglaciale and a. pullulans var. namibiae. we monitored haemolysis, cytotoxicity, antioxidant capacity and growth inhibition against three bacterial species. the haemolytic activity of a. pullulans var. pullulans exf-150 strain was due to five differen ... | 2014 | 24579879 |
| partial optimization of endo-1, 4-β-xylanase production by aureobasidium pullulans using agro-industrial residues. | objective(s) : although bacteria and molds are the pioneering microorganisms for production of many enzymes, yet yeasts provide safe and reliable sources of enzymes with applications in food and feed. | 2013 | 24570830 |
| effect of polyols on thermostability of xylanase from a tropical isolate of aureobasidium pullulans and its application in prebleaching of rice straw pulp. | in an attempt to find a thermostable xylanase enzyme for potential application in the pretreatment prior to h2o2 bleaching of paper pulp for industry, an extracellular xylanase from aureobasidium pullulans cbs 135684 was purified 17.3-fold to apparent homogeneity with a recovery yield of 13.7%. its molecular mass was approximately 72 kda as determined by sds-page. the optimal ph and temperature for activity of the purified enzyme were ph 6.0 and 70°c, respectively. the enzyme was relatively stab ... | 2014 | 24478945 |
| beneficial effects of black yeast derived 1-3, 1-6 beta glucan-nichi glucan in a dyslipidemic individual of indian origin--a case report. | dyslipidemia is a major risk factor for the development of cardiovascular diseases and statins are the common drugs used to correct dyslipidemia. herein, we report a case where the subject was a nondiabetic, dyslipidemia patient on medication with rosuvastatin. after the intake of rosuvastatin, his triglycerides decreased to a minimum of 220 mg/dl. in order to augment the action of rosuvastatin, he was advised to take 1.5 mg of nichi glucan food supplement, which is a 1,3-1,6 beta glucan derived ... | 2014 | 24409973 |
| identification of indigenous yeast flora isolated from the five winegrape varieties harvested in xiangning, china. | inoculated fermentation by selected indigenous yeast strains from a specific location could provide the wine with unique regional sensory characteristics. the identification and differentiation of local yeasts are the first step to understand the function of yeasts and develop a better strain-selection program for winemaking. the indigenous yeasts in five grape varieties, chardonnay, cabernet franc, cabernet sauvignon, marselan, and merlot cultivated in xiangning, shanxi, china were investigated ... | 2014 | 24395034 |
| insertion of a specific fungal 3'-phosphoadenosine-5'-phosphatase motif into a plant homologue improves halotolerance and drought tolerance of plants. | soil salinity and drought are among the most serious agricultural and environmental problems of today. therefore, investigations of plant resistance to abiotic stress have received a lot of attention in recent years. in this study, we identified the complete coding sequence of a 3'-phosphoadenosine-5'-phosphatase protein, aphal2, from the halotolerant yeast aureobasidium pullulans. expression of the aphal2 gene in a saccharomyces cerevisiae hal2 mutant complemented the mutant auxotrophy for meth ... | 2013 | 24349144 |
| evolution of microbiological and chemical parameters during red wine making with extended post-fermentation maceration. | the aim of the present work was to investigate the microbiological, chemical, and sensory characteristics of red wine subjected to post-fermentation maceration that was extended to 90 days. for this purpose, the 'aglianico di taurasi' grape was used as a case study. the total yeast concentration increased until day 40 of maceration and decreased thereafter, whereas the concentration of lactic acid bacteria slightly increased. dekkera/brettanomyces spp. and acetic acid bacteria were not detected. ... | 2014 | 24334093 |
| effect of uracil on pullulan production by aureobasidium pullulans cgmcc1234. | effect of uracil on the pullulan production, biomass and uridine phosphorylase (upase) activity was studied in this research. uracil was found to enhance pullulan accumulation and the addition time of uracil was crucial to pullulan production. pullulan yield of 49.07 g/l was achieved by adding 5mm uracil at 48 h, by comparison to 37.72 g/l obtained with the control. upase activity could not be detected at early growth stage of aureobasidium pullulans, but stimulated by added uracil at logarithmi ... | 2014 | 24299794 |
| an indigenous hyperproductive species of aureobasidium pullulans rylf-10: influence of fermentation conditions on exopolysaccharide (eps) production. | in recent years, a significant interest has been generated in discovering and developing exopolysaccharides (eps) produced by microorganisms, especially fungi due to their multifaceted industrial and pharmacological applications. a number of filamentous and cellular fungi have been explored for this; however, according to the existing literature, the work on exopolysaccharide production by indigenous culture on this aspect is still very less and requires a serious attention. the present work is ... | 2014 | 24293276 |
| bioconversion of industrial solid waste--cassava bagasse for pullulan production in solid state fermentation. | the purpose of the work was to produce commercially important pullulan using industrial solid waste namely cassava bagasse in solid state fermentation and minimize the solid waste disposal problem. first, influence of initial ph on cell morphology and pullulan yield was studied. effect of various factors like fermentation time, moisture ratio, nitrogen sources and particle size on pullulan yield was investigated. various supplementary carbon sources (3%, w/w) namely glucose, sucrose, fructose, m ... | 2014 | 24274475 |
| aureobasidium pullulans infection in a patient with chronic lymphocytic leukemia. | saprophytic fungi are being increasingly recognized as etiologic agents of mycoses in immunosuppressed patients. we report a case of subcutaneous infiltration by aureobasidium pullulans, likely due to traumatic inoculation, in a neutropenic patient during chemotherapy for chronic lymphocytic leukemia. the patient was treated with amphotericin b deoxycholate but was subsequently switched to itraconazole, which improved the lesion. this case highlights the importance of considering unusual fungal ... | 2013 | 24270260 |
| wine aroma improvement using a β-glucosidase preparation from aureobasidium pullulans. | microbial β-glucosidases have been used for the enhancement of wine aroma. nevertheless, few enzymes are active in the conditions of winemaking. in this work, the production of a β-glucosidase by an aureobasidium pullulans strain (ap-β-gl) isolated from grape ecosystems was evaluated. the maximum enzymatic synthesis using submerged fermentation was after 96 h of growth in complex media containing 20 g/l of cellobiose as the sole carbon source. the crude enzyme (ap-β-gl) showed optimal ph at 5.5 ... | 2013 | 23233193 |
| characterization of a newly identified rice chitinase-like protein (osclp) homologous to xylanase inhibitor. | during rice blast fungal attack, plant xylanase inhibitor proteins (xips) that inhibit fungal xylanase activity are believed to act as a defensive barrier against fungal pathogens. to understand the role of xips in rice, a xylanase inhibitor was cloned from rice. the expression of this gene was examined at the transcriptional/translational levels during compatible and incompatible interactions, and the biochemical activity of this protein was also examined. | 2013 | 23331415 |
| structural characterization of novel extracellular liamocins (mannitol oils) produced by aureobasidium pullulans strain nrrl 50380. | aureobasidium pullulans is a common, ubiquitous fungus, which is used industrially to produce the polysaccharide pullulan. we have previously shown that a. pullulans produces various heavier-than-water oils, first named here as liamocins, that accumulate in fermentations. here we report the structural characterization of four liamocins, a1, a2, b1, and b2, produced by a. pullulans strain nrrl 50380 using a combination of maldi-tof/ms, quadrupole-tof/ms, isotopic labeling, nmr, gc/ms, and classic ... | 2013 | 23435167 |
| influence of controlled ph on the activity of udpg-pyrophosphorylase in aureobasidium pullulans. | udpg-pyrophosphorylase is the key enzyme involved in pullulan biosynthesis and pullulan production by aureobasidium pullulans. in this study, effect of controlled ph on fermentation time, pullulan production, biomass, and udpg-pyrophosphorylase activity was investigated. pullulan yield increased to reach a maximum within 4 days, and maximum udpg-pyrophosphorylase activity was observed at day 3, while the biomass continued to increase until the end of the experimental period. the a. pullulans iso ... | 2013 | 23218345 |
| production of polymalic acid and malic acid by aureobasidium pullulans fermentation and acid hydrolysis. | malic acid is a dicarboxylic acid widely used in the food industry and also a potential c4 platform chemical that can be produced from biomass. however, microbial fermentation for direct malic acid production is limited by low product yield, titer, and productivity due to end-product inhibition. in this work, a novel process for malic acid production from polymalic acid (pma) fermentation followed by acid hydrolysis was developed. first, a pma-producing aureobasidium pullulans strain zx-10 was s ... | 2013 | 23436475 |
| a survey of insect assemblages responding to volatiles from a ubiquitous fungus in an agricultural landscape. | we report here a first survey of insect orientation to fungal cultures and fungal volatiles from a community ecology perspective. we tested whether volatiles from a ubiquitous yeast-like fungus (aureobasidium pullulans) are broadly attractive to insects in an agricultural landscape. we evaluated insect attraction to fungal cultures and synthetic compounds identified in fungal headspace (2-methyl-1-butanol, 3-methyl-1-butanol, 2-phenylethanol) in a spearmint (mentha spicata l.) plantation. three ... | 2013 | 23564294 |
| antidiabetic activity and molecular docking of fructooligosaccharides produced by aureobasidium pullulans in poloxamer-407-induced t2dm rats. | this study evaluated the beneficial effects of fructooligosaccharide (fos) intake from aureobasidium pullulans using poloxamer-407 (px-407) induced type 2 diabetes mellitus (t2dm) in rat. administration of fos enhanced enzymatic activities of catalase and glutathione reductase in a dose-dependent manner. significant reduction in fasting plasma triacylglycerol and very low-density lipoprotein level coupled with slight increase in fasting plasma insulin level was observed. significant decrease in ... | 2013 | 23122132 |
| induction of stilbene phytoalexins in grapevine (vitis vinifera) and transgenic stilbene synthase-apple plants (malus domestica) by a culture filtrate of aureobasidium pullulans. | products containing the epiphytic yeast aureobasidium pullulans are commercially available and applied by fruit growers to prevent several fungal and bacterial diseases of fruit trees. the proposed beneficial mechanisms relate to limitations of space and nutrients for the pathogens in presence of the rapidly proliferating yeast cells. these explanations ignore the potential of yeasts to elicit the plant's defense. our experiments aim at clarifying if an autoclaved and centrifuged suspension of a ... | 2013 | 23578977 |
| siderophore-mediated iron uptake promotes yeast-bacterial symbiosis. | in the present study, siderophore produced by the marine yeast aureobasidium pullulans was characterized as hydroxamate by chemical and bioassays. the hydroxamate assignment was supported by the appearance of peaks at 1,647.21-1,625.99 cm(-1) and at 1,435.04 cm(-1) in the infrared spectrum. the purified siderophore exhibited specific growth-promoting activity under iron-limited conditions for siderophore auxotrophic probiotic bacteria. cross-utilization of siderophore indicates a symbiotic relat ... | 2012 | 23086281 |
| production of poly(β-l-malic acid) (pma) from agricultural biomass substrates by aureobasidium pullulans. | for the first time the production of poly(β-l -malic acid) (pma) has been achieved using agricultural biomass substrates by the yeast-like fungus aureobasidium pullulans. strains nrrl y-2311-1, nrrl 50382, nrrl 50383, and nrrl 50384, representing diverse isolation sources and phylogenetic clades, produced pma from alkaline h(2)o(2)-pretreated corn fiber and wheat straw as sole carbon sources. pretreated wheat straw was better than pretreated corn fiber, and strain nrrl 50383 gave the highest ove ... | 2013 | 22955679 |
| pullulan fermentation using a prototype rotational reciprocating plate impeller. | a rotational reciprocating plate impeller prototype, designed to improve the mixing homogeneity of viscous non-newtonian fermentation broth, has been tested in pullulan fermentations. with this new impeller, the operating levels of several factors were investigated to improve pullulan production with aureobasidium pullulans atcc 42023 in a 22-l bioreactor using experimental designs. because both high molecular weight (mw) and high concentration of pullulan were desired; the exopolysaccharide (ep ... | 2013 | 22940838 |
| characterization of a glycoside hydrolase family-51 α-l-arabinofuranosidase gene from aureobasidium pullulans atcc 20524 and its encoded product. | the genomic dna and cdna encoding α-l-arabinofuranosidase were cloned from the dimorphic fungus aureobasidium pullulans atcc 20524 and sequenced. the open reading frame (2097 bp) of the α-l-arabinofuranosidase gene abfb was interrupted by five introns of 49, 49, 50, 65, and 49 bp. the gene encoded a presumed signal peptide of 17 residues and a mature protein of 682 residues with a calculated mr of 74,230 da and a theoretical isoelectric point of 4.95. glu-362 and glu-440 residues are likely invo ... | 2013 | 23597920 |
| pullulan: an exopolysaccharide and its various applications. | pullulan is a non-ionic polysaccharide obtained from fermentation of black yeast like aureobasidium pullulans and is currently exploited in food and pharmaceutical industries due to its unique characteristics. due to its properties like non-toxic, non-immunogenic, non-carcinogenic, non-mutagenic, pullulan is being explored for various biomedical applications viz., gene delivery, targeted drug therapy, tissue engineering, wound healing, and also being used in diagnostic applications like, perfusi ... | 2013 | 23618305 |
| enhancement of intestinal immune function in mice by β-d-glucan from aureobasidium pullulans adk-34. | β-glucans, glucose polymers that are the main constituents of the outer cell walls of micro-organisms such as fungi and yeast, are known to play an immunostimulatory role. we prepared β-glucan (β-(1-3),(1-6)-d-glucan) from an edible cultured fungus through fermentation techniques using a strain of aureobasidium pullulans adk-34. the purity of this β-glucan preparation (ap-fbg) was demonstrated to be high through various instrumental analyses. we then examined the effects of ap-fbg on intestinal ... | 2013 | 23672535 |
| laccases from aureobasidium pullulans. | laccases are polyphenol oxidases (ec 1.10.3.2) that have numerous industrial and bioremediation applications. laccases are well known as lignin-degrading enzymes, but these enzymes can play numerous other roles in fungi. in this study, 41 strains of the fungus aureobasidium pullulans were examined for laccase production. enzymes from a. pullulans were distinct from those from lignin-degrading fungi and associated with pigment production. laccases from strains in phylogenetic clade 5, which produ ... | 2013 | 23683702 |
| β-glucan derived from aureobasidium pullulans is effective for the prevention of influenza in mice. | β-(1→3)-d-glucans with β-(1→6)-glycosidic linked branches produced by mushrooms, yeast and fungi are known to be an immune activation agent, and are used in anti-cancer drugs or health-promoting foods. in this report, we demonstrate that oral administration of aureobasidium pullulans-cultured fluid (ap-cf) enriched with the β-(1→3),(1→6)-d-glucan exhibits efficacy to protect mice infected with a lethal titer of the a/puerto rico/8/34 (pr8; h1n1) strain of influenza virus. the survival rate of th ... | 2012 | 22844473 |
| media optimization for elevated molecular weight and mass production of pigment-free pullulan. | in this study, an aureobasidium pullulans szu 1001 mutant, deficient in pigment production, was screened by complex uv and γ-ray mutagenesis. medium composition optimization for increased pullulan molecular weight and production was conducted using this mutant. six nutrients: yeast extract, (nh4)2so4, k2hpo4, nacl, mgso4·7h2o and cacl2 were detected within pullulan production in flasks. it is shown that nacl and k2hpo4 have significant influences on molecular weight of pullulan, while yeast extr ... | 2012 | 24750882 |
| pectinolytic yeasts from viticultural and enological environments: novel finding of filobasidium capsuligenum producing pectinases. | in this study indigenous yeasts associated with wineries, grapes and malbec fermented must from san rafael viticulture region (argentina) were isolated to select pectinolytic strains for their potential use in enology. pectinolytic yeasts were identified by physiological and molecular methods. among 78 isolates, only nine were able to produce extracellular pectinases. six isolated from berry surface were identified as aureobasidium pullulans and the remaining isolates, recovered from wineries, b ... | 2014 | 23686851 |
| extracellular enzymatic activities and physiological profiles of yeasts colonizing fruit trees. | yeasts form a significant and diverse part of the phyllosphere microbiota. some yeasts that inhabit plants have been found to exhibit extracellular enzymatic activities. the aim of the present study was to investigate the ability of yeasts isolated from leaves, fruits, and blossoms of fruit trees cultivated in southwest slovakia to produce extracellular enzymes, and to discover whether the yeasts originating from these plant organs differ from each other in their physiological properties. in tot ... | 2014 | 23744750 |
| a small scale study on the effects of oral administration of the β-glucan produced by aureobasidium pullulans on milk quality and cytokine expressions of holstein cows, and on bacterial flora in the intestines of japanese black calves. | the β-(1 → 3),(1 → 6)-d-glucan extracellularly produced by aureobasidium pullulans exhibits immunomodulatory activity, and is used for health supplements. to examine the effects of oral administration of the β-(1 → 3),(1 → 6)-d-glucan to domestic animals, a small scale study was conducted using holstein cows and newborn japanese black calves. | 2012 | 22534338 |
| mechanism study of tween 80 enhancing the pullulan production by aureobasidium pullulans. | the influences of tween 80 on the pullulan production, cell growth, glucosyltransferase activity and fatty acid composition of the cells were studied. the addition of tween 80 to the culture medium significantly enhanced the pullulan production, but had no impact to biomass accumulation. glucosyltransferase activity involved in pullulan synthesis was remarkable promoted with an increase of tween 80 content. the ratio of unsaturated/saturated fatty acids contained in the cells was significantly h ... | 2013 | 23769526 |
| hypothyroidism-related onycholysis with aureobasidium pullulans colonization successfully treated with antifungal therapy. | this is a report, the first to my knowledge, of secondary nail-apparatus involvement by aureobasidium pullulans in a patient with onycholysis related to hypothyroidism. complete cure of the lesions was seen after 2 weeks of itraconazole and 2 months of local bifonazole therapy. this case raises concern about the extent of involvement of this yeast in onycholysis of diverse clinical aetiologies. | 2012 | 22439886 |
| intensification of β-poly(l: -malic acid) production by aureobasidium pullulans ipe-1 in the late exponential growth phase. | β-poly(malic acid) (pmla) has attracted industrial interest because this polyester can be used as a prodrug or for drug delivery systems. in pmla production by aureobasidium pullulans ipe-1, it was found that plma production was associated with cell growth in the early exponential growth phase and dissociated from cell growth in the late exponential growth phase. to enhance pmla production in the late phase, different fermentation modes and strategies for controlling culture redox potential (crp ... | 2012 | 22395899 |
| aureobasidium pullulans culture supernatant significantly stimulates r-848-activated phagocytosis of pma-induced thp-1 macrophages. | toll-like receptors (tlrs), which recognize a wide range of microbial pathogens and pathogen-related products, play important roles in innate immunology. macrophages have a variety of tlrs, and pathogen binding to tlr resulted in the activation of macrophages. r-848, an immune response modifier, is an analog of imidazoquinoline derivative and binds to an endosome-localized tlr to exert an anti-viral response on leukocytes. in the present study, we verified that co-treatment of r-848 with other t ... | 2013 | 23786444 |
| pullulan production by aureobasidium pullulans atcc 201253 cells adsorbed onto cellulose anion and cation exchangers. | the anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus aureobasidium pullulans atcc 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. the cells adsorbed on the triethylaminoethyl cellulose at ph 7.5 produced higher pullulan levels than those cells immobilized on phosphocellulose at ph 4.0 for 2 cycles of 168 h at 30 °c. rela ... | 2012 | 23762749 |
| lipase production by diverse phylogenetic clades of aureobasidium pullulans. | thirty-nine strains representing 12 diverse phylogenetic clades of aureobasidium pullulans were surveyed for lipase production using a quantitative assay. strains in clades 4 and 10 produced 0.2-0.3 u lipase/ml, while color variant strain nrrl y-2311-1 in clade 8 produced 0.54 u lipase/ml. strains in clade 9, which exhibit a dark olivaceous pigment, produced the highest levels of lipase, with strain nrrl 62034 yielding 0.57 u lipase/ml. by comparison, candida cylindracea strain nrrl y-17506 prod ... | 2013 | 23801121 |
| pullulan: microbial sources, production and applications. | pullulan is a water-soluble glucan gum produced aerobically by growing a yeast like fungus aureobasidium pullulans. it is a regularly repeating copolymer with the chemical structure {→6)-α-d-glucopyranosyl-(1→4)-α-d-glucopyranosyl-(1→4)-α-d-glucopyranosyl-(1→}n. thus the polysaccharide is viewed as a succession of α-(1→6)-linked (1→4)-α-d-triglucosides i.e. maltotriose (g3). pullulan have a wide range of commercial and industrial applications in many fields like food science, health care, pharma ... | 2008 | 26048217 |
| polysaccharide production by aureobasidium pullulans: factors affecting polysaccharide formation. | aureobasidium pullulans nrrl 6220 synthesized polysaccharide most actively in media containing sucrose, fructose or maltose with (nh4)2so4 (0.6 g/l) or ammonium acetate giving greatest yields of the polysaccharide. with (nh4)2so4 at ≥1.2 g/l, production of polysaccharide was decreased considerably. polysaccharide production was highest with an initial ph of 6.5 while biomass formation was better below an initial ph of 5.5. optimum phosphate concentration for polysaccharide production was 0.03 m. | 1994 | 24421090 |
| β-xylanase production by aureobasidium pullulans grown on sugars agricultural residues. | aureobasidium pullulans grew well in media containing glucose, fructose, xylan or xylose but β-xylanase was only produced with xylan or xylose. lactose and maltose were poor substrates for growth. β-xylanase production was repressed in media containing glucose or fructose along with xylose. agricultural residues, such as wheat bran, paddy husk and rice straw, could be used as carbon sources for growth and β-xylanase production of aureobasidium pullulans. tween 80 at 0.5% (v/v) increased the yiel ... | 1993 | 24420115 |
| inactivation of microorganisms by low-frequency high-power ultrasound: 2. a simple model for the inactivation mechanism. | a simple theoretical model based on shear forces generated by the collapse of the ultrasound cavities near the surface of a microorganism is proposed. this model requires two parameters which take into account the number of acoustic cavitation bubbles, and the resistance of the cell wall of the microorganism to the shear forces generated by bubble collapse. to validate the model, high-power low frequency (20 khz) ultrasound was used to inactivate two microorganisms with very different sizes, viz ... | 2014 | 23845410 |
| pullulan production and physiological characteristics of aureobasidium pullulans under acid stress. | in this study, batch processes of pullulan production by aureobasidium pullulans cctcc m 2012259 under different ph environments were evaluated. the ph of the medium decreased quickly to an acid stress condition under batch fermentation without ph control. a higher pullulan production was always obtained with a lower biomass under a given glucose concentration with constant ph control, and vice versa. based on the nonlinear regression analysis of the results obtained from diverse ph control mode ... | 2013 | 23868298 |
| combining mutualistic yeast and pathogenic virus--a novel method for codling moth control. | the combination of a pathogenic virus and mutualistic yeasts isolated from larvae of codling moth cydia pomonella is proposed as a novel insect control technique. apples were treated with codling moth granulovirus (cpgv) and either one of three yeasts, metschnikowia pulcherrima, cryptococcus tephrensis, or aureobasidium pullulans. the combination of yeasts with cpgv significantly increased mortality of neonate codling moth larvae, compared with cpgv alone. the three yeasts were equally efficient ... | 2013 | 23881444 |
| statistical studies on high molecular weight pullulan production in solid state fermentation using jack fruit seed. | the purpose of the work was to optimize the medium variables for maximizing pullulan production using jack fruit seed as a low cost substrate by aureobasidium pullulans in solid state fermentation. effects of k2hpo4, kh2po4, znso4·5h2o, mgso4·7h2o, nacl, (nh4)2so4·5h2o, yeast extract, moisture content (%, w/w) in the production medium on pullulan production were studied using plackett-burman design. production of pullulan was significantly affected by the medium variables namely kh2po4, znso4·5h ... | 2013 | 23987421 |
| role of a gata-type transcriptional repressor sre1 in regulation of siderophore biosynthesis in the marine-derived aureobasidium pullulans hn6.2. | the gata-type transcriptional repressor structural gene sre1 was isolated from both the genomic dna and mrna of the marine yeast aureobasidium pullulans hn6.2 by inverse pcr and race. an open reading frame (orf) of 1,002 bp encoding a 334 amino acid protein (a calculated isoelectric point: 8.6) with a calculated molecular weight of 35.1 kda was characterized. the corresponding gene had one single intron of 51 bp, and in its promoter two putative 5'-hgatar-3' sequences could be recognized. the de ... | 2013 | 23990279 |
| biodiversity and safety aspects of yeast strains characterized from vineyards and spontaneous fermentations in the apulia region, italy. | this work is the first large-scale study on vineyard-associated yeast strains from apulia (southern italy). yeasts were identified by internal transcribed spacer (its) ribotyping and bioinformatic analysis. the polymorphism of interdelta elements was used to differentiate saccharomyces cerevisiae strains. twenty different species belonging to 9 genera were identified. predominant on the grape surface were metschnikowia pulcherrima, hanseniaspora uvarum and aureobasidium pullulans, whereas m. pul ... | 2013 | 24010615 |
| water quality and diversity of yeasts from tropical lakes and rivers from the rio doce basin in southeastern brazil. | yeast communities were assessed in 14 rivers and four lakes from the doce river basin in brazil, during the rainy and dry seasons of the years 2000 and 2001. water samples were collected at the subsurface in all sites. the following physical and chemical parameters were measured: temperature, dissolved oxygen, ph, electrical conductivity, total phosphorus, ortho-phosphate, ammonium, nitrate, nitrite and total nitrogen and the counts of faecal coliforms and heterotrophic bacteria were carried out ... | 2012 | 24031990 |
| direct production of feruloyl oligosaccharides and hemicellulase inducement and distribution in a newly isolated aureobasidium pullulans strain. | studies were carried out to screen and identify strains that are able to directly produce ferulic oligosaccharides (fos) from wheat bran (wb). the inducement and distribution of hemicellulases from strain 2012, which was identified as a non-melanin secreting strain of aureobasidium pullulans (a. pullulans), were also determined. in a 60 g/l wb solution, a. pullulans could produce 545 nmol/l fos, 64.12 iu/ml xylanase and 0.14 iu/ml ferulic acid esterase (fae). a. pullulans was cultivated in media ... | 2014 | 24078110 |
| multiple rare opportunistic and pathogenic fungi in persistent foot skin infection. | persistent superficial skin infection caused by multiple fungi is rarely reported. recently, a number of fungi, both opportunistic and persistent in nature were isolated from the foot skin of a 24-year old male in malaysia. the fungi were identified as candida parapsilosis, rhodotorula mucilaginosa, phoma spp., debaryomyces hansenii, acremonium spp., aureobasidium pullulans and aspergillus spp., this is the first report on these opportunistic strains were co-isolated from a healthy individual wh ... | 2013 | 24175430 |
| natural antifouling compounds produced by a novel fungus aureobasidium pullulans hn isolated from marine biofilm. | a fungus, aureobasidium pullulans, was isolated from marine biofilm and identified. a bioassay-guided fractionation procedure was developed to isolate and purify antifouling compounds from a. pullulans hn. the procedure was: fermentation broth-aeration and addition of sodium thiosulfate-graduated ph and liquid-liquid extraction-spe purification-gc-ms analysis. firstly, the fermentation broth was tested for its toxicity. then it was treated with aeration and addition of sodium thiosulfate, and it ... | 2013 | 24210009 |
| an in vitro study of lactobacillus plantarum strains for the presence of plantaricin genes and their potential control of the table olive microbiota. | sixteen lactobacillus plantarum strains, isolated from fermented table olives, were studied for the presence and expression of genes involved in the production of bacteriocins, pheromones and other peptides. the presence of 13 genes that belong to pln locus was monitored, while for the study of gene expression, producer strains were cultured in growth medium with variant salinity (0, 4, 6, and 8 % nacl) and ph (3.5, 4.0, 4.5, and 6.4). the effect of producer strain on the growth of indicator mic ... | 2013 | 23224439 |
| production of pullulan by aureobasidium pullulans from asian palm kernel: a novel substrate. | production of a commercially important biodegradable polymer, pullulan, by aureobasidium pullulans from four agricultural wastes namely wheat bran, rice bran, coconut kernel and palm kernel was evaluated in solid state fermentation. under the experimental conditions, palm kernel resulted in highest concentration of pullulan (16 g/l) among the four solid substrates. optimum initial ph and moisture content for pullulan production were found out to be 6.5 and 50% respectively. 18.43 g/l of pullulan ... | 2013 | 23218356 |
| yeasts and yeast-like organisms associated with fruits and blossoms of different fruit trees. | yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various plant organs is still limited. this study focused on the diversity of yeasts and yeast-like organisms associated with matured fruits and fully open blossoms of apple, plum, and pear trees, during 2 consecutive years at 3 localities in southwest slovakia. the occurrence of yeasts and yeast-like organisms in fruit samples was 2½ times higher and the yeast community more diverse than that in blossom s ... | 2012 | 23210991 |
| aureobasidium thailandense sp. nov. isolated from leaves and wooden surfaces. | aureobasidium thailandense sp. nov. is described from cultures of material collected on leaves and wooden surfaces in thailand and the type isolate is nrrl 58539(t). phylogenetically it is distinct from other species of the genus aureobasidium. phenotypically it is distinguished by its cardinal growth temperatures, salt tolerance and production of reddish brown hyphal pigmentation in pda cultures, but micro-morphologically it is not clearly distinguishable from aureobasidium pullulans. unlike a. ... | 2013 | 23178722 |
| genome sequence of aureobasidium pullulans ay4, an emerging opportunistic fungal pathogen with diverse biotechnological potential. | aureobasidium pullulans ay4 is an opportunistic pathogen that was isolated from the skin of an immunocompromised patient. we present here the draft genome of strain ay4, which reveals an abundance of genes relevant to bioindustrial applications, including biocontrol and biodegradation. putative genes responsible for the pathogenicity of strain ay4 were also identified. | 2012 | 23104371 |
| [screening and identification of high-yield poly(β-malic acid) bacterial strain]. | to isolate and identify the high-yield poly-malic acid (pmla) bacterial strains from the nature. | 2012 | 22927080 |
| yeasts from phylloplane and their capability to produce indole-3-acetic acid. | yeasts were isolated from the phylloplane of various plant species collected from seven provinces in thailand. a total of 114 yeast strains and 10 strains of a yeast-like fungus were obtained by enrichment isolation from 91 out of 97 leaf samples (93.8 %). on the basis of the d1/d2 domain of the large subunit rrna gene sequence similarity, 98 strains were identified to be of 36 yeast species in 18 genera belonging to ascomycota viz. candida, clavispora, cyberlindnera, debaryomyces, hanseniaspora ... | 2012 | 22886557 |
| effect of β-glucan originated from aureobasidium pullulans on asthma induced by ovalbumin in mouse. | the objective of this study is to detect the effect of beta-glucan derived from aureobasidium pullulans sm-2001, a uv induced mutant of a. pullulans on the ovalbumin (ova) induced allergic asthma. the test articles were orally administered to ova-inducing asthmatic mice 4 days after sensitization for 13 days at 31.25, 62.5 or 125 mg/kg levels. three days after the ova sensitization, ten mice were selected per group based on body weight and were sacrificed three days after the ova aerosol challen ... | 2012 | 22870817 |
| new improved method for fructooligosaccharides production by aureobasidium pullulans. | fructooligosaccharides are prebiotics with numerous health benefits within which the improvement of gut microbiota balance can be highlighted, playing a key role in individual health. in this study, an integrated one-stage method for fos production via sucrose fermentation by aureobasidium pullulans was developed and optimized using experimental design tools. optimization of temperature and agitation speed for maximizing the fos production was performed using response surface methodology. temper ... | 2012 | 24750929 |
| production of pullulan by a thermotolerant aureobasidium pullulans strain in non-stirred fed batch fermentation process. | total 95 isolates of aureobasidium pullulans were isolated from different flowers and leaves samples, out of which 11 thermotolerant strains produced pullulan. one thermotolerant non-melanin pullulan producing strain, designated as rg-5, produced highest pullulan (37.1±1.0 g/l) at 42(o)c, ph 5.5 in 48h of incubation with 3% sucrose and 0.5% ammonium sulphate in a non-stirred fed batch fermentor of 6 liters capacity. the two liters of initial volume of fermentation medium was further fed with the ... | 2012 | 24031927 |
| volatile emissions from an epiphytic fungus are semiochemicals for eusocial wasps. | microbes are ubiquitous on plant surfaces. however, interactions between epiphytic microbes and arthropods are rarely considered as a factor that affects arthropod behaviors. here, volatile emissions from an epiphytic fungus were investigated as semiochemical attractants for two eusocial wasps. the fungus aureobasidium pullulans was isolated from apples, and the volatile compounds emitted by fungal colonies were quantified. the attractiveness of fungal colonies and fungal volatiles to social was ... | 2012 | 22644482 |
| [effect of oral administration of β-d-glucan from aureobasidium pullulans adk-34 on candida and mrsa infections in immunosuppressed mice]. | we examined the effect of the oral administration of β-d-glucan derived from aureobasidium pullulans adk-34 (ap-fbg) on candida albicans or methicillin-resistant staphylococcus aureus (mrsa) infection in immunosuppressed mice. mice pretreated with cyclophosphamide (cy) were intraperitoneally administered ap-fbg for 4 days and then infected with 6×10(4) c. albicans cells. in a preliminary experiment, the survival time of the candida-infected mice treated with ap-fbg was clearly prolonged. similar ... | 2012 | 22467130 |
| efficacy test of polycan, a beta-glucan originated from aureobasidium pullulans sm-2001, on anterior cruciate ligament transection and partial medial meniscectomy-induced-osteoarthritis rats. | the object of this study was to assess the efficacy of polycan from aureobasidium pullulans sm-2001, which is composed mostly of beta-1,3-1,6-glucan, on osteoarthritis (oa)-induced by anterior cruciate ligament transection and partial medial meniscectomy (aclt&pmm). three different dosages of polycan (85, 42.5, and 21.25 mg/kg) were orally administered once a day for 84 days to male rats a week after aclt&pmm surgery. changes in the circumference and maximum extension angle of each knee, and in ... | 2012 | 22370362 |
| influence of different sugars on pullulan production and activities of α-phosphoglucose mutase, udpg-pyrophosphorylase and glucosyltransferase involved in pullulan synthesis in aureobasidium pullulans y68. | effects of different sugars on pullulan production, udp-glucose level, and activities of α-phosphoglucose mutase, udpg-pyrophosphorylase and glucosyltransferase in aureobasidium pullulans y68 were examined. it was found that more pullulan was produced when the yeast strain was grown in the medium containing glucose than when it was cultivated in the medium supplementing other sugars. our results demonstrate that when more pullulan was synthesized, less udp-glucose was left in the cells of a. pul ... | 2008 | 26048225 |
| the influence of pre-treatment, temperature and calcium ions on trace element uptake by an alga (scenedesmus pannonicus subsp. berlin) and fungus (aureobasidium pullulans). | experiments were carried out in order to obtain information on the uptake characteristics of metals in the algal and fungal components of lichens. the uptake of (115)cd, (65)zn, (64)cu, (140)la, (187)w and (76)as in the alga scenedesmus pannonicus, subsp. berlin and the fungus aureobasidium pullulans was determined at ph 7 and 5 respectively, in 4 h experiments varying with respect to temperature and ca(2+) solution concentrations. furthermore, the effects of pre-rinsing the cell suspensions on ... | 1989 | 24243108 |
| humidifier lung and humidifier fever. | the aim of our study was to evaluate clinical diagnoses in symptomatic persons exposed to aerosols from humidifiers or air conditioners. in addition, we tried to identify the causative antigens. results of clinical investigations, including inhalation challenge tests, demonstrated a typical hypersensitivity pneumonitis (humidifier lung) in 9 persons and isolated systemic symptoms without significant changes in lung function and chest x-rays (humidifier fever) in 3 persons. microbiological studie ... | 1988 | 27519372 |
| the influence of ph on trace element uptake by an alga (scenedesmus pannonicus subsp. berlin) and fungus (aureobasidium pullulans). | experiments were carried oud in order to obtain information on the uptake characteristics of metals in the algal and fungus components of lichens. the uptake of (115)cd, (65)zn, (64)cu, (140)la, (187)w, (75)se, and (76)as in the alga scenedesmus pannonicus, subsp. berlin and the fungus aureobasidium pullulans was determined during 11 days of incubation at ph 5 and ph 7. the results indicate different acid-sensitivity of the alga and the fungus. the metal uptake in the alga was generally faster a ... | 1988 | 24248629 |
| influence of zinc, lead, and cadmium pollutants on the microflora of hawthorn leaves. | transect studies were conducted to determine the relative effects of zinc, lead, and cadmium pollution on microorganisms occurring on hawthorn leaves at varying distances from a smelting complex.sporobolomyces roseus was absent from the most heavily contaminated leaves but, although lead was inhibitory, other environmental factors were also important in determining its overall population level. conversely,aureobasidium pullulans and nonpigmented yeasts showed a significant partial positive corre ... | 1980 | 24227130 |