| a rapid colorimetric assay of killer toxin activity in yeast. | the pale yellow redox indicator 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (mtt) is reduced to a dark blue end-product, mtt-formazan, by the mitochondrial dehydrogenases of living cells. mtt reduction can be measured spectrophotometrically at a wavelength of 570 nm and a method is described to assay the cidal activity of williopsis mrakii killer toxin against sensitive cells of candida glabrata. the mtt assay is rapid, quantitative and compares favourably with traditional plat ... | 1994 | 8056291 |
| dominance of metallothionein in metal ion buffering in yeast capable of synthesis of (gamma ec)ng isopeptides. | the relationship of yeast metallothionein (mt) and (gamma ec)ng isopeptides (phytochelatins) in metal ion buffering was assessed. the effect of constitutive expression of yeast metallothionein (mt) genes on accumulation of metal-(gamma ec)ng isopeptide (phytochelatin) complexes was analyzed in candida glabrata and schizosaccharomyces pombe cultures incubated in the presence of cadmium salts. constitutive expression of the saccharomyces cerevisiae mt (cup1) gene inhibited the accumulation of meta ... | 1994 | 8063718 |
| rapid presumptive identification of medically relevant yeasts to the species level by polymerase chain reaction and restriction enzyme analysis. | a method for the rapid presumptive differentiation of a panel of 12 clinically relevant yeasts to the species level was developed on the basis of evaluation by the polymerase chain reaction (pcr) of the gene coding for the small ribosomal subunit 18s-rrna. the method involved restriction enzyme analysis of pcr products obtained with primers common to all fungi. using six restriction enzymes, alui, bani, bbsi, draii, eco147i and nhei, characteristic pcr-restriction enzyme patterns were obtained f ... | 1994 | 8064542 |
| mixed cu+ and zn2+ coordination in the dna-binding domain of the amt1 transcription factor from candida glabrata. | amt1 is the transcription factor required for cu-induced expression of metallothionein genes in the yeast candida glabrata. the copper-binding, dna-binding domain of amt1 has been purified after expression of an amt1 synthetic gene in bacteria and was confirmed as active in a gel shift assay. the cu-activated amt1 was shown to contain a cu(+)-thiolate tetracopper center and a single zn2+ site. amt1 is purified as a cu-zn protein from bacterial cultures grown in the presence of cuso4. chemical an ... | 1994 | 8068632 |
| correlation between antifungal susceptibility testing of candida isolates from patients with hiv infection and clinical results after treatment with fluconazole. | in an open-label controlled study 23 hiv-infected patients (cdc iv a-e) with documented oropharyngeal candidosis were treated with 100 mg fluconazole orally over 5 days (53 episodes; 1-6 treatments/patient). efficacy data were compared with a control group of 21 patients who received treatment for 10-21 days with 100 mg fluconazole for candidosis. candida isolates were repeatedly recovered from patients before and after treatment with fluconazole and antifungal susceptibility testing (microbroth ... | 1994 | 8070927 |
| the contribution of electrostatic forces to the process of adherence of candida albicans yeast cells to substrates. | the contribution of electrostatic interactions to the adherence process of candida albicans and other candida species was investigated by mixing cationic or anionic exchange resins possessing free -coo- or -nh+4 groups, respectively, on their surface. the adherence process of yeast cells to the anionic exchange resin is a saturable event that is essentially complete by 60 min. there is no measurable interaction of yeast cells with the cationic exchange resin. all clinical isolates of c. albicans ... | 1994 | 8076801 |
| controlled clinical evaluation of isolator and esp aerobic blood culture systems for detection of bloodstream infections. | a controlled clinical evaluation comparing the isolator system (wampole laboratories, cranbury, n.j.) and the esp 80a blood culture bottle in the automated esp system (difco laboratories, detroit, mich.) was performed with 10,535 blood culture sets from patients with suspected septicemia. of 1,150 positive cultures, 844 positive cultures from 285 patients with 394 septic episodes fulfilled the study criteria for minimum blood sample requirements in each system and clinical significance of isolat ... | 1994 | 8077401 |
| the structure of the small mitochondrial dna of kluyveromyces thermotolerans is likely to reflect the ancestral gene order in fungi. | mapping the 23-kb circular mitochondrial dna from the yeast kluyveromyces thermotolerans has shown that only one change occurs in the gene order in comparison to the 19-kb mtdna of candida (torulopsis) glabrata. sequence analysis of the mitochondrially encoded cytochrome oxidase subunit 2 gene reveals that despite their conserved gene order, the two small genomes are more distantly related than larger mtdna molecules with multiple rearrangements. this result supports a previous observation that ... | 1994 | 8083884 |
| fluconazole susceptibilities of candida species and distribution of species recovered from blood cultures over a 5-year period. | the distribution and fluconazole susceptibilities of candida species isolated over a 5-year period were investigated. susceptibilities were determined by using a new microtiter procedure and the national committee for clinical laboratory standards (nccls) proposed standard. the new method correlated well with the nccls proposed standard and gave very clear end points. results indicate that there are species-related differences in mics as reflected in the mics for 90% of species tested. candida a ... | 1994 | 8092849 |
| oligonucleotide fingerprinting of isolates of candida species other than c. albicans and of atypical candida species from human immunodeficiency virus-positive and aids patients. | oligonucleotide fingerprinting of genomic dna from oral isolates of four different candida species other than c. albicans and atypical chlamydospore-positive isolates from human immunodeficiency virus (hiv)-positive individuals and aids patients was investigated as a means for differentiating between isolates within individual species. oligonucleotides composed of simple repetitive sequence motifs, including (gaca)4, (gata)4, (ggat)4, (gtg)5, and (gt)8, all yielded fingerprints suitable for stra ... | 1993 | 8103773 |
| nucleotide sequence analysis of the 5.8s rdna and adjacent its2 region of candida albicans and related species. | we have determined the nucleotide sequence for the dna encoding the 5.8s rnas and downstream internal transcribed spacer (its2) regions for candida albicans and the taxonomically related species c. parapsilosis, c. tropicalis, c. glabrata and c. krusei. phylogenetic analysis of all known fungal 5.8s rna sequences revealed a close relationship between c. tropicalis and c. parapsilosis, and to a lesser extent c. albicans within the yeast-like fungi. this group can itself be delineated from predomi ... | 1993 | 8109169 |
| changes in the spectrum of fungal isolates: results from clinical specimens gathered in 1987/88 compared with those in 1991/92 in the university hospital göttingen, germany. | in the university hospital in göttingen, the spectra of fungal species in clinical specimens of respiratory secretions, bronchial secretions and urine were compared over periods of 15 months (10/87 to 12/88 and 1/91 to 3/92) before and after the introduction of fluconazole. the following changes could be demonstrated: 1. in all specimens analysed the number of candida albicans isolates decreased, while the number of candida tropicalis isolates remained almost unchanged. 2. during the observation ... | 1993 | 8114803 |
| in vitro susceptibility of fungal isolates of clinically important specimens to itraconazole, fluconazole and amphotericin b. | the in vitro activity of itraconazole, fluconazole and amphotericin b was tested against 207 yeast strains and 3 aspergillus fumigatus strains isolated from blood. the other 42 a. fumigatus strains were selected from respiratory tract infections. a microdilution method was employed to determine the inhibitory concentrations to restrain 70% of isolate growth (ic30). the inhibition concentrations of amphotericin b are all around the value 0.25 mg/l with a deviation of two concentration degrees, in ... | 1994 | 8131639 |
| phagocytosis of medically important yeasts by polymorphonuclear leukocytes. | phagocytosis is a critical function of polymorphonuclear leukocytes in the control of mycotic infections. by using a modified fluorescence quenching assay to distinguish between attached and ingested organisms, we determined the percent phagocytosis of several medically important yeasts. the percentages of phagocytosis of serum-opsonized candida albicans, candida tropicalis, candida parapsilosis, and torulopsis glabrata were all comparable at 37 degrees c. by comparison, there was significantly ... | 1994 | 8132358 |
| cytotoxic and antibacterial labdane-type diterpenes from the aerial parts of cistus incanus subsp. creticus. | seven labdane-type diterpenoids were isolated from the leaves of cistus incanus subsp. creticus; their structures were established by spectroscopic means. all compounds were tested in vitro for their cytotoxicity against three cell line systems: kb, p-388; and nsclc-n6. their antibacterial and antifungal activities were tested against staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosae, enterobacter cloacae, escherichia coli, klebsiella pneumonia, torulopsis glabrata, sacc ... | 1994 | 8134413 |
| in vitro antifungal activity of triterpenoid saponins. | the antifungal activity of triterpenoid saponins, with hederagenin or oleanolic acid as aglycone, was investigated in vitro by the agar dilution method. monodesmosidic hederagenin derivatives were shown to exhibit a broad spectrum of activity against yeast as well as dermatophyte species. alpha-hederin was the most active compound, and candida glabrata was the most susceptible strain. the structure-activity relationships are discussed. | 1994 | 8134417 |
| interaction of staphylococcus aureus with human neutrophils and the down-regulation of tnf receptors. | we have shown previously that pre-exposure of neutrophils to tnf significantly enhanced their killing of opsonized staphylococcus aureus. we now demonstrate that the ability of tnf to enhance the bactericidal activity is dependent on preincubation time; enhancement was still evident when tnf and bacteria were added simultaneously to neutrophils but if tnf addition was delayed by 5 min, no enhancement was seen. evidence is presented that suggests that this could be related to a down-regulation of ... | 1994 | 8144967 |
| suppression of fungal growth exhibited by pseudomonas aeruginosa. | three surgery patients were monitored postoperatively, with particular reference to lung infection. in each case there was a clinical impression that pseudomonas aeruginosa suppressed the growth of candida albicans in patients with clinically significant lung infections from whom both of these organisms were isolated from serial sputum samples. regrowth of c. albicans after p. aeruginosa eradication occurred in two patients, despite fluconazole therapy, to which both c. albicans isolates were su ... | 1994 | 8150966 |
| accurate initiation of mrna synthesis in extracts from schizosaccharomyces pombe, kluyveromyces lactis and candida glabrata. | we demonstrate the successful adaptation to other yeast species of a protocol previously described for production of transcriptionally active whole cell extracts from saccharomyces cerevisiae (woontner and jaehning, 1990, j. biol. chem. 265, 8979-8982). extracts prepared from schizosaccharomyces pombe, kluyveromyces lactis and candida glabrata were all capable of initiating transcription from a template containing the s. cerevisiae cyc1 tata box fused to a g-less cassette. transcription in all o ... | 1993 | 8154183 |
| torulopsis glabrata pelvic abscess and fungemia. | serious infections caused by torulopsis glabrata, once rarely encountered, have become common over the last 3 decades. the most frequent manifestations of serious fungal infections include septicemia, endocarditis, hepatosplenic infections, and meningitis. we report a case of fungemia and pelvic abscess caused by t glabrata following gynecologic surgery. | 1994 | 8159387 |
| clinical comparison of the baxter microscan yeast identification panel and the vitek yeast biochemical card. | to determine the reliability of the baxter microscan yeast identification panel, processed by the walkaway-96, and the vitek yeast biochemical card, 150 clinical yeast isolates (30 candida albicans, 67 candida species, not albicans, 26 torulopsis glabrata, 13 cryptococcus neoformans, 4 saccharomyces cerevisiae, 6 trichosporon beigelii, 3 rhodotorula species, and 1 geotrichum species) were tested on both systems. results were compared with those obtained by the api 20c and the appearance of yeast ... | 1994 | 8160633 |
| [autochthonous yeasts isolated in tenerife wines and their influence on ethyl acetate and higher alcohol concentrations analyzed by gas chromatography]. | a taxonomic study of yeasts present on tenerife wines, (tacoronte-acentejo specific denomination) has been carried out. nine species of the genera: saccharomyces, torulaspora, brettanomyces, kluyveromyces, debaryomyces, saccharomycodes, hansenula, pichia and candida have been isolated. parallely we analysed volatile compounds of the wines such as ethyl acetate, methanol, isobutanol and amylic alcohols by gas chromatography. appreciable quantities of ethyl acetate were detected due to the low fer ... | 1993 | 8172687 |
| a system for gene cloning and manipulation in the yeast candida glabrata. | the opportunistic pathogenic yeast, candida (torulopsis) glabrata, is an asexual imperfect fungus that exists largely as a haploid. besides being a clinically important pathogen, this yeast also provides a model system for understanding basic biological mechanisms such as metal-activated metallothionein-encoding gene transcription. to facilitate molecular genetic studies in c. glabrata, we isolated a strain auxotrophic for uracil biosynthesis. the ura- mutation could be functionally complemented ... | 1994 | 8181748 |
| the effect of fluconazole prophylaxis on fungal colonization in neutropenic cancer patients. bone marrow transplantation team. | the impact of prophylaxis with 400 mg/day fluconazole on fungal colonization at different body sites was assessed in a randomized, double-blind, placebo controlled study among patients with leukaemia and those undergoing bone marrow transplantation. the study drug was given throughout the period of neutropenia and samples were obtained at weekly intervals. of the 23 patients in each group, 11 of those given fluconazole and 12 placebo recipients were colonized at entry. the commonest sites were t ... | 1994 | 8182012 |
| role of cds quantum crystallites in cadmium resistance in candida glabrata. | glutathione-related peptides (gamma-glu-cys)ngly, trivially known as phytochelatins (pcs), sequester cd(ii) and other heavy metals in all plants and some yeasts. however, the metal resistance levels may depend on factors such as the pc concentrations, their chain length and ability to incorporate labile sulfide. we show here that a highly cd(ii)-resistant mutant of yeast candida glabrata exhibited cd(ii)-dependent formation of extremely high levels of pc-coated cds quantum crystallites. the cds ... | 1994 | 8185567 |
| vaginal yeast infections in diabetic women. | two hundred and three diabetic women (89 with and 114 without genital symptoms) were examined for the presence of yeasts and trichomonas vaginalis. yeasts were isolated from the vaginas of 35.5% of patients and were more common in the symptomatic group (48.0%) than the asymptomatic group (25.4%; p < 0.05). candida albicans was isolated from 12.8% of all patients and showed a significant association with pruritus vulvae (p < 0.05). a significant association was also shown between the presence of ... | 1993 | 8191325 |
| potential use of bact/alert automated blood culture system for antifungal susceptibility testing. | a simple, rapid susceptibility test is needed to determine the possible resistance of yeasts to commonly used antifungal agents. the bact/alert automated blood culture system was evaluated as one technology for development of such a test. yeast nitrogen base was used as the growth medium, and amphotericin b was the test antifungal agent. isolates of various candida species, torulopsis glabrata, saccharomyces cerevisiae, and cryptococcus neoformans were evaluated. the results suggest that detecti ... | 1994 | 8195407 |
| single-stranded regions in yeast mitochondrial dna revealed by pulsed-field gel electrophoresis. | the klenow fragment of e. coli dna polymerase together with pulsed-field gel electrophoresis (pfge) have been used to investigate the presence of single-stranded dna (ssdna) regions in yeast (torulopsis glabrata) dna. electrophoretic profiles of total dna from rho+ (wild type) and rho0 (no mitochondrial dna) strains demonstrate that this method mediates the incorporation of labelled datp into mitochondrial dna (mtdna), but not into chromosomal dna. the majority of ssdna (> 62%) has been found as ... | 1993 | 8199217 |
| selection of candida glabrata in pediatric bone marrow transplant recipients receiving fluconazole. | we observed an alarmingly high rate (5/16 patients; 31.3%) of orointestinal colonization with candida glabrata--often in high numbers--in pediatric bone marrow transplant recipients receiving fluconazole as antifungal prophylaxis. this selection is probably due to the intrinsically low susceptibility of c. glabrata to fluconazole. | 1994 | 8204447 |
| fluconazole in children: first experience with prophylaxis in chemotherapy-induced neutropenia in pediatric patients with cancer. | in an open multicenter, prospective study fluconazole (flu) was administered to prevent fungal infection in 22 neutropenic, 2- to 14-year-old children. of 4 failures, one was infected with an aspergillus spp., one with candida krusei and one with candida (torulopsis) glabrata. one failure was of bacterial origin. those patients with mycoses who failed on fluconazole were successfully treated with other antifungals. in 4 patients transient elevation of liver enzymes was observed. this is the firs ... | 1993 | 8222873 |
| [in vitro susceptibility to fluconazole of fungal strains freshly isolated from child patients with deep-seated mycoses]. | the susceptibility to fluconazole (flcz) were evaluated for 40 strains of 8 yeast species and 4 strains of 3 aspergillus species, all of which were isolated from clinical specimens during clinical trials of flcz in child patients performed from january 1991 to january 1993 in this country. the in vitro activity of flcz against yeast isolates and aspergillus isolates were measured using the microdilution method with semisolid saamf (synthetic amino acid medium fungal) agar and the macrodilution m ... | 1993 | 8230734 |
| a pseudocyst infected by torulopsis glabrata: a unique problem. | | 1993 | 8237952 |
| natural history of bloodstream infections in a burn patient population: the importance of candidemia. | because of a perceived increase in candida bloodstream infections in our burn unit, we retrospectively reviewed all the microbiologic data and the medical records of 209 patients with burns admitted during a 42-month period. twice weekly burn wound cultures demonstrated that candida species were the tenth most frequently isolated organisms (69/191 patients, 36%). despite the low frequency of isolation from burn wounds, candida species were the most common organisms found in blood cultures and ur ... | 1993 | 8239049 |
| association of torulopsis glabrata infections with fluconazole prophylaxis in neutropenic bone marrow transplant patients. | because the use of fluconazole prophylaxis had been associated with an increased rate of candida krusei infections at the john hopkins oncology center, early empiric amphotericin b plus flucytosine were given to febrile neutropenic patients colonized by c. krusei. by this practice, the proportion of fungemias attributable to c. krusei was low (12.5%) in patients receiving fluconazole over a 6-month interval. however, torulopsis (candida) glabrata assumed a much higher proportion of fungemias (75 ... | 1993 | 8239594 |
| fluconazole resistance in candida glabrata. | we report a case of infection with candida glabrata in which the organism became resistant to fluconazole and in which pre- and posttreatment isolates were available for comparison. the organism was cross-resistant to ketoconazole and itraconazole, in common with other azole-resistant yeasts. fluconazole was a potent inhibitor of cytochrome p-450-dependent 14 alpha-sterol demethylase (p-450dm) in lysates of cells from both susceptible and resistant cultures (50% inhibitory concentration, 0.2 mic ... | 1993 | 8239613 |
| hiv-infected children in the pediatric emergency department. | forty-three nonhemophiliac, confirmed hiv-positive children followed by the children's hospital aids program made 184 visits to the children's emergency department (ed) during 1988 and 1989. the mean age was 30 +/- 28 months with a median of 25 months, a mode of 10 months, and a range from two days to 19 years. cd4 counts from within six months of the visit were available in 87% and were low enough to require pneumocystis carinii pneumonia prophylaxis under current guidelines in 52%. chief compl ... | 1993 | 8247930 |
| in vitro studies of a new antifungal triazole, d0870, against candida albicans, cryptococcus neoformans, and other pathogenic yeasts. | we investigated the effects of various assay conditions on the activity of d0870 against seven species of fungi in the broth macrodilution testing procedure proposed by the national committee for clinical laboratory standards (nccls). multivariate analysis demonstrated that endpoint definition, starting inoculum size, medium composition, type of buffer, and length of incubation, but not ph or temperature, had significant effects on results. increasing the inoculum from 10(2) to 10(5) yeast cells ... | 1993 | 8257134 |
| mac1, a nuclear regulatory protein related to cu-dependent transcription factors is involved in cu/fe utilization and stress resistance in yeast. | the related transcription factors ace1 of saccharomyces cerevisiae and amt1 of candida glabrata are involved in copper metabolism by activating the transcription of copper metallothionein genes. ace1 and amt1 are 'copper-fist' transcription factors which possess a conserved cysteine-rich copper binding domain required for dna binding. here we report the identification of a nuclear protein from s. cerevisiae, mac1, whose n-terminal region is highly similar to the copper and dna binding domains of ... | 1993 | 8262047 |
| new milk medium for germ tube and chlamydoconidia production by candida albicans. | a new medium consisting of uht milk, tween 80 and agar is described for the development of both germ tube and chlamydoconidia by candida albicans. in total 172 isolates from clinical specimens, including c. albicans (112), c. guilliermondii (4), c. krusei (3), c. parasilopsis (16). c. tropicalis (28), torulopsis glabrata (6) and trichosporon beigellii (3), were examined in this medium by using the standard method. a higher percentage (98.2%) of germ tube production by c. albicans was found in th ... | 1993 | 8264773 |
| prevention of invasive fungal infections in patients with neoplastic disease. | invasive fungal infections are important causes of morbidity and mortality among patients with neoplastic diseases, particularly those with protracted granulocytopenia, those receiving corticosteroids, and those undergoing allogeneic bone marrow transplantation. these mycoses are often difficult to diagnose early, and their treatment is frequently unsuccessful. antifungal compounds have been used in studies of a variety of preventive strategies including prophylaxis, early empirical therapy, emp ... | 1993 | 8274613 |
| use of a monoclonal antibody in a dot immunobinding assay for detection of a circulating mannoprotein of candida spp. in neutropenic patients with invasive candidiasis. | a dot immunobinding assay for the detection of a circulating mannoprotein (mp) antigen of candida species in the sera of neutropenic patients in a hematological setting is described. the technique is based on the use of a monoclonal antibody which recognizes an oligomannoside epitope shared by distinct mp of pathogenic candida species. the sensitivity of the assay for antigen detection in serum was between 2 and 5 ng/ml, and mps from candida albicans, candida tropicalis, torulopsis glabrata, and ... | 1993 | 8308105 |
| molecular epidemiology of recurrent oral candidiasis in human immunodeficiency virus-positive patients: evidence for two patterns of recurrence. | the causative strains in 22 patients with recurrent oral candidiasis were examined using two dna probes (a histoplasma capsulatum dna probe that cross-hybridizes with candida albicans and a c. albicans strain-specific probe derived from repetitive sequence dna). c. albicans was the causative organism in all 22 initial episodes of infection and was also obtained from 17 patients with recurrent oral disease. molecular analysis showed that in 11 cases, the same isolate was identified in each episod ... | 1993 | 8335986 |
| torulopsis glabrata in the neonate: an emerging fungal pathogen. | fungi are becoming increasingly common nosocomial pathogens in the neonatal intensive care patient. the fungus torulopsis glabrata, a common skin inhabitant, is a potential pathogen in the high-risk neonate. in this report we have reviewed the cases of two infants in which systemic t glabrata infection was diagnosed. one patient survived without apparent sequelae; the other died before diagnosis and initiation of therapy. five other cases of systemic infection by t glabrata in neonates have been ... | 1993 | 8351565 |
| rapid transcriptional autoregulation of a yeast metalloregulatory transcription factor is essential for high-level copper detoxification. | copper detoxification in the yeast candida glabrata is carried out in large part by a family of metallothionein (mt) genes: a unique mt-i gene, a tandemly amplified mt-iia gene, and a single unlinked mt-iib gene. in response to elevated environmental copper levels, members of this mt gene family are transcriptionally activated by a copper-dependent, sequence-specific dna-binding transcription factor, amt1. amt1 shares several structural and functional features with the saccharomyces cerevisiae c ... | 1993 | 8370529 |
| [a case of endocarditis caused by candida glabrata]. | a 45-year-old diabetic man had aortic valve replacement for candida glabrata endocarditis. postoperatively he was treated with fluconazole and amphotericin b. despite therapy, c. glabrata was isolated from his blood. he collapsed suddenly and died of heart failure 70 days after operation. autopsy revealed suture line insufficiency and perivalvular leakage. on histologic examination many colonies of fungi were found in the aortic valvular annulus and an embolus to the mesenteric artery. | 1993 | 8377315 |
| management of persistent vulvo vaginal candidosis due to azole-resistant candida glabrata. | case report--subjects--three cases are described of long-standing vaginal candidosis due to candida glabrata. these had failed to respond to local and systemic antifungals. in each case the infecting strain appeared resistant to a range of azole drugs in vitro. clinical course--case one--this patient recovered following prolonged treatment with oral itraconazole in combination with oral and vaginal nystatin. case two. yeasts were eradicated from this patient following cyclical treatment with ora ... | 1993 | 8389723 |
| comparison of restriction enzyme analysis versus pulsed-field gradient gel electrophoresis as a typing system for torulopsis glabrata and candida species other than c. albicans. | candida species have recently emerged as important nosocomial pathogens. because of the lack of a reliable system for detecting differences within the same species, little is known about the epidemiology of infection with candida species. we describe a typing system for torulopsis glabrata and the non-c. albicans candida species that uses contour-clamped homogeneous electric field electrophoresis (chef), a version of pulsed-field gradient gel electrophoresis, and compared it with restriction enz ... | 1993 | 8396585 |
| multicenter evaluation of a broth macrodilution antifungal susceptibility test for yeasts. | thirteen laboratories collaborated to optimize interlaboratory agreement of results of a broth macrodilution procedure for testing three classes of antifungal drugs against pathogenic yeasts. the activities of amphotericin b, flucytosine, and ketoconazole were tested against 100 coded isolates of candida albicans, candida tropicalis, candida parapsilosis, candida lusitaniae, torulopsis (candida) glabrata, and cryptococcus neoformans. two starting yeast inoculum sizes (5 x 10(4) and 2.5 x 10(3) c ... | 1993 | 8431016 |
| azelaic acid has antimycotic properties in vitro. | azelaic acid is a therapeutic agent with well-known antibacterial properties, but its antimycotic effect has not yet been investigated systematically. in this study we have used an agar dilution technique to test the inhibitory effect of azelaic acid upon common dermatophytes, scopulariopsis brevicaulis, candida albicans, candida glabrata and pityrosporum ovale. as a result, the growth of dermatophytes and s. brevicaulis was suppressed by 0.56% azelaic acid and that of p. ovale by 1%. by 4% azel ... | 1993 | 8435518 |
| vaginitis due to saccharomyces cerevisiae: epidemiology, clinical aspects, and therapy. | vaginitis due to saccharomyces species is extremely rare. nine patients with 20 vaginal isolates of saccharomyces cerevisiae who presented with either asymptomatic vaginal colonization or symptomatic vaginitis indistinguishable from that caused by candida albicans are described. all patients had a history of chronic or recurrent vaginitis, and all but two had systemic or local predisposing factors. in vitro tests of antimycotic sensitivity revealed reduced susceptibility of s. cerevisiae to the ... | 1993 | 8448324 |
| fungemia due to torulopsis glabrata. | torulopsis glabrata is a yeast ordinarily considered nonpathogenic. systemic infection with this yeast occurs in patients who are debilitated, immunosuppressed, diabetic, or receiving multiple antibiotics. we have presented a case of fungemia due to t glabrata in a previously healthy person. the predisposing condition resulting in debility and predisposition to fungemia was major vascular surgery. treatment with amphotericin b eradicated the fungemia. | 1993 | 8451678 |
| diagnosis of invasive candidiasis by a dot immunobinding assay for candida antigen detection. | a dot immunobinding assay which uses a polyclonal rabbit anti-candida immunoglobulin g as the primary antibody and colloidal gold coated with goat anti-rabbit immunoglobulin g as the secondary antibody for the detection of candida cytoplasmic antigens is described. it was able to detect as little as 1 ng of total candida protein per ml when a cytoplasmic extract of candida albicans was seeded into buffer and 10 ng/ml when the same extract was seeded into pooled human serum. serial serum samples ... | 1993 | 8458945 |
| thrombin-induced rabbit platelet microbicidal protein is fungicidal in vitro. | platelet microbicidal protein (pmp) is released from platelets in response to thrombin stimulation. pmp is known to possess in vitro bactericidal activity against staphylococcus aureus and viridans group streptococci. to determine whether pmp is active against other intravascular pathogens, we evaluated its potential fungicidal activity against strains of candida species and cryptococcus neoformans. anionic resin adsorption and gel electrophoresis confirmed that the fungicidal activity of pmp re ... | 1993 | 8460923 |
| controlled comparison of the bactec high-blood-volume fungal medium, bactec plus 26 aerobic blood culture bottle, and 10-milliliter isolator blood culture system for detection of fungemia and bacteremia. | the bactec high-blood-volume fungal medium (hbv-fm) (becton dickinson diagnostic instrument systems, sparks, md.) was compared with the isolator (is) tube and the bactec plus 26 (bp26) blood culture bottle for the ability to recover fungi from the blood of adult patients suspected of having fungemia. a total of 6,836 blood culture sets that fulfilled criteria for inclusion in the study were received. three separate comparisons were performed: 4,907 hbv-fm versus is, 4,886 bp26 versus hbv-fm, and ... | 1993 | 8463399 |
| rapid, polymerase chain reaction-based identification assays for candida species. | polymerase chain reaction (pcr) amplification of specific regions in the genomes of a variety of lower eukaryotes permits rapid identification of these microorganisms. first, on the basis of the presence of both constant and variable regions in the small subunit (ssu) rrna, a nested pcr for direct identification of various candida species can be designed. amplification of the entire ssu rrna gene and subsequent reamplification of variable sequences within the v4 domains of these pcr products wer ... | 1993 | 8463403 |
| investigation of a killer strain of zygosaccharomyces bailii. | the yeast zygosaccharomyces bailii strain 412 was found to liberate a killer toxin (kt412) lethal to sensitive strains of saccharomyces cerevisiae and candida glabrata. culture supernatants of the killer strain were concentrated by ultrafiltration and the extracellular protein was purified by gel filtration and ion-exchange chromatography. gel filtration and sds-page of the electrophoretically homogeneous killer protein indicated an apparent molecular mass of 10 kda. the killer toxin kt412 is pr ... | 1993 | 8473858 |
| percutaneous nephrostomy for nonoperative management of fungal urinary tract infections. | percutaneous nephrostomy has proved to be an important modality in the nonoperative treatment of bacterial pyonephrosis. the role of this technique in the management of fungal pyonephrosis continues to evolve. the authors retrospectively reviewed their experience with percutaneous nephrostomy in the management of fungal pyonephrosis. | 1993 | 8481584 |
| characterization of two monoclonal antibodies against secretory proteinase of candida tropicalis dsm 4238. | two murine igm monoclonal antibodies (mab; mt1 and mt2), which were produced against the secretory aspartic proteinase of candida tropicalis dsm 4238, are described. both antibodies reacted with the native and denatured conformations of the homologous proteinase antigen but showed different patterns of reactivity with other related proteinases (candida albicans cbs 2730, serotype a; c. albicans atcc 48867, serotype b; candida parapsilosis dsm 4237) and with porcine pepsin. neither of the antibod ... | 1993 | 8483055 |
| [evaluation of a new automated system for the determination of antifungal sensitivity]. | to evaluate a new semi-automatized system (atb-fungus, bio mérieux, spain) for antifungal in vitro susceptibility testing in yeast. | 1993 | 8499512 |
| regulation of metallothionein genes by the ace1 and amt1 transcription factors. | the amt1 metalloregulatory trans-acting factor from candida glabrata was found to functionally mimic the ace1 metalloregulatory trans-acting factor from saccharomyces cerevisiae in the copper-induced expression of the chromosomal s. cerevisiae metallothionein gene. plasmid constructs with promoters of various metal-inducible genes fused to the bacterial beta-galactosidase (lacz) reporter gene were used in s. cerevisiae to evaluate the roles of ace1 and amt1 in mediating metal-stimulated expressi ... | 1993 | 8509391 |
| cloning of the candida glabrata trp1 and his3 genes, and construction of their disruptant strains by sequential integrative transformation. | the candida glabrata (cg) trp1 and his3 genes have been isolated by complementation of the saccharomyces cerevisiae (sc) trp1 and his3 mutants, respectively. cg trp1 encodes a polypeptide of 217 amino acids (aa), whose aa sequence is 58% identical to that of sc trp1. cg his3 encodes a polypeptide of 210 aa, whose aa sequence is 73% identical to that of the sc his3. both cg trp1 and his3 were disrupted by sequential integrative transformation where the sc ura3 was used as a selection marker for t ... | 1995 | 8522176 |
| candida glabrata epididymo-orchitis: an unusual infection rapidly cured with surgical and antifungal treatment. | we report a case of epididymo-orchitis and secondary fungaemia caused by candida (previously torulopsis) glabrata in an 83-year-old male patient who had diabetes mellitus, urinary outflow obstruction and previous bladder malignancy. we believe this to be a previously unreported site of infection with c. glabrata and we describe its rapid and successful treatment with combination anti-fungal therapy. | 1995 | 8522840 |
| catheter-related intracardiac thrombosis: a rare complication of candida glabrata sepsis. | a 58-year-old patient suffered from persistent candida glabrata fungaemia. transoesophageal echocardiography detected a central venous catheter-related intracardiac thrombosis. cardiotomy permitted the removal of the catheter and its adherent clot. candida glabrata was cultured from the thrombus. | 1995 | 8533535 |
| [intestinal obstruction and sepsis caused by torulopsis glabrata]. | a 27-year-old woman previously diagnosed of aplastic anemia secondary to treatment with gold salts for rheumatoid arthritis, presented with an episode of intestinal occlusion with acute renal failure. a ct scan revealed dilated intestinal loops, thickening of the ileum wall without cecal involvement, and multiple punctuate lesions (micro-abcesses) of liver, spleen and kidneys. at laparotomy, one meter of proximal jejunum was resected. the cultures of jejunal biopsy specimens yielded torulopsis g ... | 1995 | 8534539 |
| antifungal susceptibility testing of yeasts: evaluation of technical variables for test automation. | the technical parameters for antifungal susceptibility testing with candida species were reexamined to determine the optimal conditions for testing with semiautomated preparations of broth microdilution cultures, automated spectrophotometric readings of the cultures, and dose-response and endpoint determinations by means of a computer spreadsheet. tests were based on proposed standard method m27p of the national committee for clinical laboratory standards for antifungal agents. rpmi 1640 broth w ... | 1995 | 8540715 |
| improved detection of medically important fungi by immunoperoxidase staining with polyclonal antibodies. | this study was performed to identify pathological fungi of eight species [aspergillus fumigatus, candida albicans, torulopsis (candida) glabrata, cryptococcus neoformans, fusarium anthophilum, rhizopus oryzae, sporothrix schenckii and trichosporon beigelii] in formalin-fixed, paraffin-embedded tissue sections by indirect immunoperoxidase staining. mature albino rabbits were immunized with formalin-killed organisms. antibodies were prepared by precipitation. immunoperoxidase staining was applied ... | 1995 | 8548126 |
| autoactivation by a candida glabrata copper metalloregulatory transcription factor requires critical minor groove interactions. | rapid transcriptional autoactivation of the candida glabrata amt1 copper metalloregulatory transcription factor gene is essential for survival in the presence of high extracellular copper concentrations. analysis of the interactions between purified recombinant amt1 protein and the amt1 promoter metal regulatory element was carried out by a combination of missing-nucleoside analysis, ethylation interference, site-directed mutagenesis, and quantitative in vitro dna binding studies. the results of ... | 1996 | 8552101 |
| in vitro antifungal activity of polyporaceae against yeasts and dermatophytes. | the antifungal activity of 38 polyporaceae sensu lato against yeasts and dermatophytes was tested in vitro by the agar dilution method. strains were typed organisms and clinical isolates. in this first report, pycnoporellus fulgens (fr.) donk was found to be the most active species against pathological fungi and showed broad-spectrum antifungal activity against yeasts (candida albicans, candida glabrata) and dermatophytes (trichophyton mentagrophytes, trichophyton rubrum, microsporum canis, micr ... | 1995 | 8559195 |
| dna amplification for the in vitro detection of candida albicans in head and neck squamous cell carcinomas. | dna was extracted from whole cells of candida albicans and digested with hindiii restriction enzyme. after electrophoresis in a segment of the lane containing between 800 and 1200 base pairs (bp) of dna fragments, a 1.1-kilobase (kb) fragment was found that hybridizes to biopsied tumor cells from head and neck squamous cell carcinomas (scc). from the nucleotide sequence of the putative gene locus, primers were synthesized for use in a polymerase chain reaction (pcr) with dna extracted from 18 sc ... | 1995 | 8562037 |
| torulopsis glabrata: azole susceptibilities by microdilution colorimetric and macrodilution broth assays. | fluconazole and itraconazole mics were determined by both the standard macrodilution method of the national committee for clinical laboratory standards and a colorimetric broth microdilution method for 140 isolates of torulopsis (candida) glabrata obtained over a 15-year period. using the method of the national committee for clinical laboratory standards the mics at which 90% of isolates are inhibited (mic50) for all isolates were 32 and 1.6 micrograms/ml for fluconazole and itraconazole, respec ... | 1995 | 8567892 |
| comparative evaluation of macrodilution and alamar colorimetric microdilution broth methods for antifungal susceptibility testing of yeast isolates. | a comparative evaluation of the macrodilution method and the alamar colorimetric method for the susceptibility testing of amphotericin b, fluconazole, and flucytosine was conducted with 134 pathogenic yeasts. the clinical isolates included 28 candida albicans, 17 candida tropicalis, 15 candida parapsilosis, 12 candida krusei, 10 candida lusitaniae, 9 candida guilliermondii, 18 torulopsis glabrata, and 25 cryptococcus neoformans isolates. the macrodilution method was performed and interpreted acc ... | 1995 | 8567901 |
| a murine model of candida glabrata vaginitis. | vaginal candida glabrata infections have increased significantly in recent years and are particularly common in women with uncontrolled diabetes mellitus. efforts to understand the pathogenesis and treatment of this infection have been hindered by the lack of experimental animal models. before onset of hyperglycemia, nonobese diabetic (nod) mice inoculated intravaginally with clinical c. glabrata isolates were shown to support high vaginal titers of c. glabrata for > 14 days with evidence for su ... | 1996 | 8568305 |
| molecular probes for diagnosis of fungal infections. | we have developed 21 specific nucleic acid probes which target the large subunit rrna genes from aspergillus flavus, aspergillus fumigatus, aspergillus glaucus, aspergillus niger, aspergillus terreus, blastomyces dermatitidis, candida albicans, candida (torulopsis) glabrata, candida guilliermondii, candida kefyr, candida krusei, candida lusitaniae, candida parapsilosis, candida tropicalis, coccidioides immitis, cryptococcus neoformans var. gattii, cryptococcus neoformans var. neoformans, filobas ... | 1995 | 8576345 |
| use of chromagar candida medium for isolation of yeasts from dental samples. | a new differential medium, chromagar candida, for the isolation of clinically important yeasts was investigated to determine its usefulness in facilitating the study of oral yeasts. the recovery of yeasts on the medium was not significantly different from the recovery on sabouraud dextrose agar. the identities of 450 green colonies on chromagar candida, presumptively identified as candida albicans on the basis of the manufacturer's instructions, were confirmed by testing for beta-n-acetylgalacto ... | 1995 | 8576366 |
| [rational use of antimycotics against yeast infections]. | in clinical medicine mere colonization with yeasts is often hardly to be discriminated from true infection. thus, a clear-cut separation of preventive from therapeutic use of antimycotics is not possible in practical medicine. the problem is that on the one hand one has no exact diagnosis of yeast infection, but on the other hand best therapeutic results are obtained when the drugs are given as early as possible. in comparison to the huge number of antibacterial compounds, the members of antimic ... | 1995 | 8582736 |
| parotitis due to torulopsis glabrata. | | 1995 | 8589177 |
| deletion of the candida glabrata erg3 and erg11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility. | we have cloned and sequenced the structural genes encoding the delta 5,6 sterol desaturase (erg3 gene) and the 14 alpha-methyl sterol demethylase (erg11 gene) from candida glabrata l5 (leu2). single and double mutants of these genes were created by gene deletion. the phenotypes of these mutants, including sterol profiles, aerobic viabilities, antifungal susceptibilities, and generation times, were studied. strain l5d (erg3 delta::leu2) accumulated mainly ergosta-7,22-dien-3 beta-ol, was aerobica ... | 1995 | 8593007 |
| in vitro activity of clotrimazole for candida strains isolated from recent patient samples. | minimum inhibitory concentrations (mics) of clotrimazole (cas 23593-75-1, bay 5097, clo) were determined for 142 clinical candida isolates obtained between 1992 and 1994, including the species candida albicans (96 strains), candida glabrata (12 strains), candida krusei (12 strains), and candida tropicalis (12 strains). for some of the candida isolates of all four species, the mics of amphotericin b and fluconazole were also determined. no mics of clo of > 4 micrograms/ml were found for all four ... | 1995 | 8595097 |
| effect of saliva composition on growth of candida albicans and torulopsis glabrata. | candida albicans and torulopsis glabrata are the most prevalent yeasts in humans. the majority harbor c. albicans in the oral cavity, but only a few develop oral candidiasis. we have sought a possible relationship between indigenous salivary constituents, including antimicrobial and nutritive factors, and the growth rate and/or viability of inoculated fungi in glucose-supplemented sterilized saliva. stimulated whole saliva was collected from 30 healthy donors. saliva samples were sterilized, sup ... | 1995 | 8602336 |
| torulopsis glabrata cystitis--a report of three cases. | | 1994 | 8613165 |
| itraconazole susceptibilities of fluconazole susceptible and resistant isolates of five candida species. | the in-vitro susceptibilities of 1380 isolates of five candida species were determined in order to establish whether isolates resistant to fluconazole were cross-resistant to itraconazole. ic50 values were determined by a broth microdilution method. 690 candida albicans isolates, seven candida glabrata isolates, seven candida krusei isolates, 120 candida parapsilosis isolates and 37 candida tropicalis isolates were susceptible to both fluconazole (ic50 < or = 32 mg/l) and itraconazole (ic50 < or ... | 1995 | 8626259 |
| oral fluconazole versus amphotericin b bladder irrigation for treatment of candidal funguria. | a randomized trial was conducted to compare amphotericin b bladder irrigation (ambbi) with oral fluconazole in terms of efficacy and safety in the treatment of candidal funguria. fifty-three patients with two consecutive positive funal cultures of urine were randomized to undergo ambbi (50 mg/l over 24 hours or 50 mg/l for 7 days) or to receive fluconazole (200 mg/d for 7 days). urinary catheters were changed upon entry into the study and following therapy. blood and urine specimens were obtaine ... | 1995 | 8645847 |
| torulopsis glabrata-infected pancreatic pseudocysts. diagnosis and treatment. | torulopsis glabrata, a fungus commensal with the human gastrointestinal tract, so far has not been recognized as a cause of pancreatic sepsis. we report the cases of two patients with pancreatic pseudocysts that became infected with t. glabrata. a 20-year-old woman 6 weeks postpartum had acute gallstone pancreatitis complicated by pseudocyst formation and pancreatic sepsis. pseudocyst fluid obtained at cystogastrostomy showed a pure culture of t. glabrata. a 52-year-old man with multiple medical ... | 1995 | 8648059 |
| new and emerging yeast pathogens. | the most common yeast species that act as agents of human disease are candida albicans, candida tropicalis, candida glabrata, candida parapsilosis, and cryptococcus neoformans. the incidence of infections by other yeasts has increased during the past decade. the most evident emerging pathogens are malassezia furfur, trichosporon beigelii, rhodotorula species, hansenula anomala, candida lusitaniae, and candida krusei. organisms once considered environmental contaminants or only industrially impor ... | 1995 | 8665465 |
| the changing face of candidemia: emergence of non-candida albicans species and antifungal resistance. | to assess the changing epidemiology of candidemia in the 1990s, to evaluate the clinical implications for the presence of non-candida albicans in blood, and to evaluate the presence of antifungal resistance in relation to prior antifungal administration. | 1996 | 8678081 |
| candida glabrata: in vitro susceptibility of 84 isolates to eight antifungal agents. | the in vitro susceptibility of 84 isolates of candida glabrata from patients treated at the university hospital of the canary islands to eight antifungal agents (amphotericin b, itraconazole, fluconazole, miconazole, clotrimazole, tioconazole and econazole) has been studied using the broth dilution micro-method. among the eight antifungal agents tested, the smaller geometric mean corresponded to tioconazole, econazole, clotrimazole and miconazole. in contrast with fluconazole, a greater geometri ... | 1996 | 8697885 |
| candida albicans stimulates cytokine production and leukocyte adhesion molecule expression by endothelial cells. | endothelial cells have the potential to influence significantly the host immune response to blood-borne microbial pathogens, such as candida albicans. we investigated the ability (of this organism to stimulate endothelial cell responses relevant to host defense in vitro. infection with c. albicans induced endothelial cells to express mrnas encoding e-selectin, intercellular adhesion molecule 1, vascular cell adhesion molecule 1, interleukin 6, interleukin 8, monocyte chemoattractant protein 1, a ... | 1996 | 8698486 |
| enhanced effectiveness of copper ion buffering by cup1 metallothionein compared with crs5 metallothionein in saccharomyces cerevisiae. | the bakers' yeast saccharomyces cerevisiae contains a metallothionein (mt) gene family comprised of the amplified cup1 locus and the single copy crs5 gene. we demonstrate that cup1 plays the dominant role in copper detoxification. a single copy of cup1 was far more effective in conferring copper resistance than was crs5. the cup1 promoter contributes to this resistance; in a promoter exchange experiment, the crs5 mt conferred strong copper resistance when its expression was driven by the cup1 pr ... | 1996 | 8702498 |
| vesicular eruption on the scalp. varicella-zoster virus with torulopsis glabrata colonization. | | 1996 | 8712849 |
| in-vitro antifungal activity of sertaconazole, bifonazole, ketoconazole, and miconazole against yeasts of the candida genus. | the in-vitro antifungal activity of sertaconazole against 110 strains of candida yeasts (50 candida albicans, 15 candida glabrata, 2 candida guilliermodii, 8 candida krusei, 1 candida kefyr, 8 candida parapsilosis and 26 candida tropicalis) was assessed in comparison with bifonazole, ketoconazole, econazole and miconazole. the majority of the strains were clinical isolates; some reference strains were included. a commercial agar diffusion method (neosensitabs, rosco, taastrup, denmark) in shadom ... | 1996 | 8722548 |
| extracellular addition of a domain of hiv-1 vpr containing the amino acid sequence motif h(s/f)rig causes cell membrane permeabilization and death. | vpr is a virion-associated protein of human immunodeficiency virus type 1 (hiv-1) whose function in acquired immune deficiency syndrome (aids) has been uncertain. we previously employed yeast as a model to examine the effects of vpr on basic cellular functions; intracellular vpr was shown to cause cell-growth arrest and structural defects, and these effects were caused by a region of vpr containing the sequence hfrigcrhsrig. here we show that peptides containing the h(s/f)rig amino acid sequence ... | 1996 | 8730861 |
| [fluconazole in treatment of urinary candidiasis. experience with 24 patients]. | since fluconazole achieves high urine concentrations, we assessed its usefulness in the treatment of urinary candidiasis. we studied 24 patients (8 male) aged 23 to 97 years old, that presented pyuria with a negative urine culture for bacteria and fungal colony counts in urine of 10(4) cfu/ml or more. isolated strains were candida albicans in 20 cases, candida kefyr in one case, candida glabrata in one case and candida spp in two cases. all patients were treated with fluconazole in doses of 50 t ... | 1995 | 8733268 |
| fluconazole treatment of candidal infections caused by non-albicans candida species. | fluconazole is an effective alternative to amphotericin b for the treatment of serious infections caused by candida albicans. through a literature survey of candidal infections caused by non-albicans candida spp., 43 cases treated with fluconazole were found. the most common causative organisms were candida parapsilosis (14 patients), candida glabrata (12 patients), and candida tropicalis (11 patients). the dose of fluconazole varied from 50 to 400 mg daily. the median duration of treatment was ... | 1996 | 8740860 |
| application of chromagar candida for rapid screening of clinical specimens for candida albicans, candida tropicalis, candida krusei, and candida (torulopsis) glabrata. | chromagar candida is a new differential culture medium that allows selective isolation of yeasts and simultaneously identifies colonies of candida albicans, c. tropicalis, and c. krusei. we evaluated the use of this medium with 316 yeast isolates including 247 isolated directly on chromagar from clinical material. over 95% of stock and clinical isolates of c. albicans, c. tropicalis, and c. krusei were correctly identified on the basis of colony morphology and pigmentation on chromagar. addition ... | 1996 | 8748273 |
| analysis of copper-induced metallothionein expression using autonomously replicating plasmids in candida glabrata. | candida glabrata strains and a stable plasmid were developed that were suitable for analysis of copper-inducible expression from promoters of the three metallothionein (mt) genes. the two homologous mtii genes, mtiia and mtiib, encode the same polypeptide but are differentially induced by copper salts. mtiib is more highly inducible than mtiia and cells harboring a single mtiib exhibit a greater resistance to copper salts compared to cells harboring a single mtiia. the differential copper induci ... | 1995 | 8750238 |
| use of terbinafine in hiv-positive subjects: pilot studies in onychomycosis and oral candidiasis. | study 1. eighteen hiv-positive caucasian homosexual men with initial positive fungal microscopy were recruited into this prospective, dual-centre, open-label study. they received a once-daily oral dose of 250 mg terbinafine for 12 weeks. eight were subsequently excluded after screening cultures proved negative. the mean cd4 count of the 10 evaluable subjects was 302/mm3. all 10 positive fungal cultures were confirmed as trichophyton rubrum. using an intention-to-treat analysis, healthy unaffecte ... | 1996 | 8763464 |
| torulopsis glabrata spinal osteomyelitis involving two contiguous vertebrae. a case report. | a case report and literature review of the five previous case reports. | 1996 | 8779020 |
| species-specific identification of candida krusei by hybridization with the ckf1,2 dna probe. | the species specificity of the candida krusei dna fingerprinting probe ckf1,2 has been investigated. a total of 149 pathogenic and nonpathogenic fungal and bacterial dnas were screened with ckf1,2. the probe was cold labeled with peroxidase, and its specificity was assessed by using southern blot, dot blot, and colony blot hybridization. its sensitivity was determined by dot blot hybridization. the ckf1,2 probe proved to be species specific. it hybridized with dna for the 112 c. krusei strains s ... | 1996 | 8784578 |
| torulopsis pneumonia. a case report and review of the literature. | torulopsis glabrata is a rare cause of pneumonia in immunocompromised patients. we herein describe the case of an elderly man who presented with fulminant torulopsis pneumonia and septic shock leading to death. we then review the literature, describe the clinical syndrome, and delineate an approach to diagnosis and treatment of torulopsis pneumonia. | 1996 | 8797445 |
| evaluation of a commercial medium for identification of candida species. | chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ... | 1996 | 8801088 |