| dynamics of calcium during in vitro microspore embryogenesis and in vivo microspore development in brassica napus and solanum melongena. | calcium is widely known to have a role as a signaling molecule in many different processes, including stress response and activation of the embryogenic program. however, there are no direct clues about calcium levels during microspore embryogenesis, an experimental process that combines a developmental switch toward embryogenesis and the simultaneous application of different stressing factors. in this work, we used fluoforte, a calcium-specific fluorescent vital dye, to track by confocal microsc ... | 2017 | 28736567 |
| [changes in weight of fresh and dried vegetables on cooking]. | the present study represents part of a more important work relative to normal food refuse and change of weight of varied food according to different ways of cooking. the study has been realized on vegetables and some fruits of frequent utilization in the population. it was accomplished by 25 voluntary housekeepers from paris and marseille during a whole year according to precise instructions about the method of weighings, every vegetable having to be weighed, three times at different intervals, ... | 1976 | 970838 |
| investigation of orthologous pathogen recognition gene-rich regions in solanaceous species. | pathogen receptor proteins such as receptor-like protein (rlp), receptor-like kinase (rlk), and nucleotide-binding leucine-rich repeat (nlr) play a leading role in plant immunity activation. the genome architecture of such genes has been extensively investigated in several plant species. however, we still know little about their elaborate reorganization that arose during the plant speciation process. using recently released pepper and eggplant genome sequences, we were able to identify 1097 path ... | 2017 | 28742982 |
| in silico analysis reveals widespread presence of three gene families, mapk, mapkk and mapkkk, of the mapk cascade from crop plants of solanaceae in comparison to the distantly-related syntenic species from rubiaceae, coffee. | mitogen-activated protein kinases (mapks) are an important family of genes which play roles in vital plant processes, and they also help in coping against various kinds of environmental stresses including abiotic as well as biotic factors. the advancement of genomics calls for the annotation, identification, and detailed processing of the essential gene families in plants in order to provide insights into the importance of their central roles as well as for providing the basis for making their g ... | 2017 | 28603666 |
| assessing the pathogenic ability of ralstonia pseudosolanacearum (ralstonia solanacearum phylotype i) from ornamental rosa spp. plants. | ralstonia pseudosolanacearum (ralstonia solanacearum phylotype i) isolates found in stunted, yellowing, and wilted ornamental rose (rosa spp.) were assessed for their pathogenic ability in two rose cultivars (cv. "armando" and cv. "red naomi") and in four solanaceous crops: tomato (solanum lycopersicum cv. "money maker"), tobacco (nicotiana tabacum cv. "white burley"), eggplant (solanum melongena cv. "black beauty") and sweet pepper (capsicum annum cv. "yolo wonder"). significant differences wer ... | 2017 | 29163615 |
| the effect of cooking method upon the titratable acidity of a popular vegetarian dish--scope for reducing its erosive potential? | the purpose of this in vitro study was to determine the effect of cooking method on the erosive potential of ratatouille. two cooking methods, stewing and oven roasting, were applied to standardised ingredients taken from the same fruits and vegetables. the resultant dishes were liquidised and diluted with 100 mls of distilled water. five 25 ml samples of each group were titrated to ph 7.0 against 0.1 molar sodium hydroxide. in order to ascertain the relative contribution of each ingredient each ... | 2006 | 16599095 |
| a plant metabolic activation system from persea americana with cytochrome p450-dependent and peroxidase activities. | microsomal fractions from different tissues of various plants (potato, cauliflower, aubergine, avocado pear, courgette, cucumber, banana, kiwi and strawberry) were prepared and their content of cytochrome p-450 (cyt-p450) determined. a s117 fraction from persea americana (avocado pear) presented the highest content of cyt-p450. as a consequence of these data, we have developed and characterized this fraction as a plant metabolic activation system. the p. americana s117, used in this work, contai ... | 1995 | 7770073 |
| bioaccumulation of heavy metals and two organochlorine pesticides (ddt and bhc) in crops irrigated with secondary treated waste water. | four crop plants oryza sativa (rice), solanum melongena (brinjal), spinacea oleracea (spinach) and raphanus sativus (radish) were grown to study the impact of secondary treated municipal waste water irrigation. these plants were grown in three plots each of 0.5 ha, and irrigated with secondary treated waste water from a sewage treatment plant. sludge from the same sewage treatment plant was applied as manure. cultivated plants were analyzed for accumulation of heavy metals and pesticides. result ... | 2009 | 18654831 |
| activation of anthocyanin synthesis genes by white light in eggplant hypocotyl tissues, and identification of an inducible p-450 cdna. | eggplant seedlings (solanum melongena) grown under red light irradiation showed a normal morphology with green, fully expanded cotyledons. when the seedlings grown under red light were irradiated with ultraviolet-containing white light, anthocyanin synthesis was induced in the hypocotyl tissues, especially when a uv light supplement was added. the accumulation of pigments was closely associated with the expression of genes involved in flavonoid synthesis. these genes include chalcone synthase (c ... | 1993 | 8260632 |
| defining cooking salt intakes for patient counselling and policy making. | the role of salt (nacl) in the development of high blood pressure has been a matter of debate, however, the intersalt study showed that sodium (na) intake in various areas of the world is related to the slope of blood pressure with age. accurate amounts of the total salt intake or that coming from a particular source are needed, both, for physicians who need to consider the salt intake of their patients and for public health workers who are in charge of the implementation of public health progra ... | 1995 | 9230039 |
| application of coupled-column liquid chromatography combined with post-column photochemically induced fluorimetry derivatization and fluorescence detection to the determination of pyrethroid insecticides in vegetable samples. | this study reports the first application of coupled-column liquid chromatography-photochemically induced fluorimetry-fluorescence detection (lc-lc-pif-fd), demonstrating its potential for the quantitative and selective detection of seven pyrethroids in vegetable samples such as cucumber, green bean, tomato and aubergine. an internal surface reversed-phase (isrp) column coupled to a c18 column for analyte clean-up and determination were used, respectively. in comparison with a c18 column, the isr ... | 2005 | 15702307 |
| coupled-column liquid chromatography method with photochemically induced derivatization for the direct determination of benzoylureas in vegetables. | the coupled-column (lc-lc) system, consisting of a first column packed with internal surface reversed phase (isrp) (50 x 4.6mm id) and a chrompack c18 (100 x 4.6 mm id) as second column, allowed the simultaneous determination of five benzoylurea insecticides in dichloromethane (ch2cl2) extracts of vegetable samples without any clean-up step. this system was combined with a photochemically induced fluorescence (pif) post-column derivatization in order to provide strongly fluorescent photoproducts ... | 2004 | 15537073 |
| sequential injection analysis with lab-at-valve (si-lav) for the determination of solasodine in solanum species. | the development of sequential injection analysis with lab-at-valve (lav) semi-automated system on-line liquid-liquid extraction is demonstrated for spectrophotometric determination of solasodine in various solanum species fruits. the main proposed is semi-automated extractive determination of solasodine using methyl orange as colorimetric reagent. after optimization of the system, sample, reagent and organic solvent were sequentially aspirated into an extraction coil connected to the center of a ... | 2010 | 20188963 |
| world vegetable center eggplant collection: origin, composition, seed dissemination and utilization in breeding. | eggplant is the fifth most economically important solanaceous crop after potato, tomato, pepper, and tobacco. apart from the well-known brinjal eggplant (solanum melongena l.), two other under-utilized eggplant species, the scarlet eggplant (s. aethiopicum l.) and the gboma eggplant (s. macrocarpon l.) are also cultivated. the taxonomy and identification of eggplant wild relatives is challenging for breeders due to the large number of related species, but recent phenotypic and genetic data and c ... | 2017 | 28970840 |
| multivariate analysis as a key tool in chemotaxonomy of brinjal eggplant, african eggplants and wild related species. | the brinjal eggplant (solanum melongena l.) is an important vegetable species worldwide, while african eggplants (s. aethiopicum l., s. macrocarpon l.) are indigenous vegetable species of local significance. taxonomy of eggplants and their wild relatives is complicated and still unclear. hence, the objective of the study was to clarify taxonomic position of cultivars and landraces of brinjal, its wild relatives and african eggplant species and their wild ancestors using chemotaxonomic markers an ... | 2017 | 28910606 |
| variations in the structure and transcription of the mitochondrial atp and cox genes in wild solanum species that induce male sterility in eggplant (s. melongena). | in order to determine the molecular basis of cytoplasmic male sterility (cms) in alloplasmic lines of eggplant, the genomic structures and transcription patterns of mitochondrial atp synthase subunit (atp) and cytochrome oxidase subunit (cox) genes were studied for wild and cultivated eggplants. alloplasmic eggplant lines with cytoplasms of wild solanum species showing either anther indehiscent type of cms or non-pollen production type of cms were studied with the cultivated eggplant solanum mel ... | 2013 | 23604528 |
| issr and isozyme characterization of androgenetic dihaploids reveals tetrasomic inheritance in tetraploid somatic hybrids between solanum melongena and solanum aethiopicum group gilo. | gene exchanges between solanum melongena and its allied relative solanum aethiopicum are a crucial prerequisite for introgression of useful traits from the allied species into the cultivated eggplant. in order to evaluate the extent of genetic recombination between the 2 species, biochemical and molecular markers were employed. a dihaploid population obtained through anther culture of the corresponding tetraploid somatic hybrids was genetically analyzed. the extent of disomic/tetrasomic inherita ... | 2008 | 18245797 |
| proximate and mineral compositions of cooked and uncooked solanum melongena. | the proximate and mineral compositions of four different varieties of cooked and uncooked solanum melongena were determined. except for the percentage moisture, the levels of crude protein, crude fat, total ash and carbohydrate content were generally higher in uncooked samples than in cooked samples. solanum macrocarpum (uncooked) has the highest moisture content with an average value of 73.9% and 84.5% for coat and flesh respectively, while the coat of solanum sessiliflorum and flesh of solanum ... | 2002 | 11939104 |
| production and characterization of fertile somatic hybrids of eggplant (solanum melongena l.) with solanum aethiopicum l. | in order to produce fertile somatic hybrids, mesophyll protoplasts from eggplant were electrofused with those from one of its close related species, solanum aethiopicum l. aculeatum group. on the basis of differences in the cultural behavior of the parental and hybrid protoplasts, 35 somatic hybrid plants were recovered from 85 selected calli. when taken to maturity either in the greenhouse or in the field, the hybrid plants were vigorous, all rapidly overtopping parental individuals. the putati ... | 1993 | 24196058 |
| a putative r3 myb repressor is the candidate gene underlying atroviolacium, a locus for anthocyanin pigmentation in tomato fruit. | anthocyanins are potential health-promoting compounds in the human diet. the atv (atroviolacium) locus, derived from the wild tomato species solanum cheesmaniae, has been shown to enhance anthocyanin pigmentation in tomato fruit when it co-exists with either the aft (anthocyanin fruit) or the abg (aubergine) locus. in the present study, the atv locus was fine-mapped to an approximately 5.0-kb interval on chromosome 7. a putative r3 myb repressor was identified in this interval and is hereby desi ... | 2017 | 29186488 |
| performance of pseudapanteles dignus (hymenoptera: braconidae), a natural enemy of tuta absoluta (lepidoptera: gelechiidae) in eggplant. | pseudapanteles dignus (muesebeck; hymenoptera: braconidae) is an american endoparasitoid that attacks the south american tomato leafminer tuta absoluta (meyrick; lepidoptera: gelechiidae). the interaction between p. dignus and t. absoluta in tomato (solanum lycopersicum l.; solanales: solanaceae) crops has demonstrated that this enemy exhibits some desirable ecological traits as an effective biological control agent of this pest. with the aim of extending the use of p. dignus to other solanaceou ... | 2017 | 29294007 |
| volatile-mediated attraction of greenhouse whitefly trialeurodes vaporariorum to tomato and eggplant. | the behavior of the greenhouse whitefly, trialeurodes vaporariorum westwood (hemiptera: aleyrodidae), is known to be affected by plant volatile cues, but its attraction or repellent to specific volatile cues has not been deeply studied yet. therefore, the aim of our study was to identify the most attractive plant among cultivars of tomato (solanum lycopersicum) and eggplant (solanum melongena) to evaluate the volatiles of plants to identify the chemical compound(s) that attract t. vaporariorum. ... | 2017 | 28775733 |
| sulphur alters chromium (vi) toxicity in solanum melongena seedlings: role of sulphur assimilation and sulphur-containing antioxidants. | the present study investigates modulation in hexavalent chromium [cr(vi) 25 μm] toxicity by sulphur (s; 0.5, 1.0 and 1.5 mm s as low (ls), medium (ms) and high sulphur (hs), respectively) in solanum melongena (eggplant) seedlings. biomass accumulation (fresh and dry weights), photosynthetic pigments, photosynthetic oxygen evolution and s content were declined by cr(vi) toxicity. furthermore, fluorescence characteristics (jip-test) were also affected by cr(vi), but cr(vi) toxicity on photosystem ... | 2017 | 28088020 |
| transcriptome analysis of solanum melongena l. (eggplant) fruit to identify putative allergens and their epitopes. | eggplant is the third most important solanaceae crop after tomato and potato, particularly in india and china. a transcriptome analysis of eggplant's fruit was performed to study genes involved in medicinal importance and allergies. illumina hiseq 2000 system generated 89,763,638 raw reads (~18 gb) from eggplant. high quality reads (59,039,694) obtained after trimming process, were assembled into a total of 149,224 non redundant set of transcripts. out of 80,482 annotated sequences of eggplant f ... | 2016 | 26424595 |
| host plant choice experiments of colorado potato beetle (coleoptera: chrysomelidae) in virginia. | field and laboratory-choice experiments were conducted to understand aspects of host plant orientation by the colorado potato beetle, leptinotarsa decemlineata say (coleoptera: chrysomelidae), in virginia. in laboratory bioassays, l. decemlineata oriented to volatiles emitted by potato, solanum tuberosum l., foliage over both tomato, lycopersicon esculentum l., and eggplant, solanum melongena l., foliage, and eggplant over tomato foliage, all of which had been mechanically damaged. field choice ... | 2008 | 18613587 |
| macrolophus pygmaeus (hemiptera: miridae) population parameters and biological characteristics when feeding on eggplant and tomato without prey. | the population parameters and biological characteristics of the predator macrolophus pygmaeus rambur when feeding on eggplant, solanum melongena l., and tomato, lycopersicon esculentum (miller) (both solanaceae), without access to prey, were investigated at several temperatures. the experiments were conducted at 15, 20, 25, and 30 degrees c, 65 +/- 5% rh, and a photoperiod of 16:8 (l:d) h. females oviposited on both plant species at all temperatures, with the exception of tomato at 30 degrees c. ... | 2004 | 15384340 |
| jasmonic acid inducible aspartic proteinase inhibitors from potato. | a new cdna clone coding for an aspartic proteinase inhibitor homologue was isolated from a potato tuber cdna library. southern blot analysis was used to study the structural diversity of the aspartic proteinase inhibitor gene family in several species of the solanaceae. the existence of sequence-homologous genes was confirmed in the genomic dna of different potato cultivars (solanum tuberosum l. cv. désirée, pentland squire and igor), tomato (lycopersicon esculentum mill.), aubergine (s. melonge ... | 1997 | 9055446 |
| the role of irradiation dose and dna content of somatic hybrid calli in producing asymmetric plants between an interspecific tomato hybrid and eggplant. | highly asymmetric somatic hybrid plants were obtained by peg/dmso fusion of gamma-irradiated mesophyll protoplasts of the kanamycin-resistant (kmr(+)) interspecific hybrid lycopersicon esculentum x l. pennellii (ep) with mesophyll protoplasts of solanum melongena (eggplant, e). elimination of the ep chromosomes was obtained by irradiating the donor genome with different doses of gamma rays (100, 250, 500, 750 and 1000 gy). the selection of somatic hybrid calli was based on kanamycin resistance; ... | 1996 | 24166550 |
| potential of delphinidin-3-rutinoside extracted from solanum melongena l. as promoter of osteoblastic mc3t3-e1 function and antagonist of oxidative damage. | increasing evidence suggests the potential use of natural antioxidant compounds in the prevention/treatment of osteoporosis. this study was undertaken to investigate the effects of purified delphinidin-3-rutinoside (d3r), isolated from solanum melongena l., on osteoblast viability and differentiation in basal conditions and its ability to protect mc3t3-e1 cells against oxidative damage induced by tert-butyl hydroperoxide (t-bhp). | 2018 | 29372310 |
| genotyping by sequencing highlights a polygenic resistance to ralstonia pseudosolanacearum in eggplant (solanum melongena l.). | eggplant cultivation is limited by numerous diseases, including the devastating bacterial wilt (bw) caused by the ralstonia solanacearum species complex (rssc). within the rssc, ralstonia pseudosolanacearum (including phylotypes i and iii) causes severe damage to all solanaceous crops, including eggplant. therefore, the creation of cultivars resistant to r. pseudosolanacearum strains is a major goal for breeders. an intraspecific eggplant population, segregating for resistance, was created from ... | 2018 | 29370090 |
| disentangling pectic homogalacturonan and rhamnogalacturonan-i polysaccharides: evidence for sub-populations in fruit parenchyma systems. | the matrix polysaccharides of plant cell walls are diverse and variable sets of polymers influencing cell wall, tissue and organ properties. focusing on the relatively simple parenchyma tissues of four fruits - tomato, aubergine, strawberry and apple - we have dissected cell wall matrix polysaccharide contents using sequential solubilisation and antibody-based approaches with a focus on pectic homogalacturonan (hg) and rhamnogalacturonan-i (rg-i). epitope detection in association with anion-exch ... | 2018 | 29291850 |
| the pax protein eyegone (eyg) interacts with the pi-rna component aubergine (aub) and controls egg chamber development in drosophila. | the eyegone (eyg) gene encodes eyg, a transcription factor of the pax family with multiple roles during drosophila development. eyg has been shown to be nuclear in the cells where it functions. in this report we describe a new functional cytoplasmic distribution of eyg during egg chamber development in the female ovarioles. the protein is present from the germarium until stage 10 of cyst development. the majority of egg chambers that develop in the absence of eyg arrest their development before ... | 2017 | 29278721 |
| leaf chemical compositions of different eggplant varieties affect performance of bemisia tabaci (hemiptera: aleyrodidae) nymphs and adults. | the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae) meam1 is a serious pest of many crops worldwide, and its control mostly depends on insecticides. one of the most preferred host plants of b. tabaci is eggplant, solanum melongena, although preferences among different cultivars of the whiteflies vary. we hypothesized that certain nutritional and defensive chemicals of plant leaves, such as nitrogen, glucose, fructose, sucrose, amino acids, total phenolic components, and ... | 2017 | 29272524 |
| distinct fitness costs associated with the knockdown of rnai pathway genes in western corn rootworm adults. | rna interference (rnai) based approaches can potentially be used to control insect pests. these approaches may depend on the usage of microrna (mirna) or double stranded rna (dsrna) mediated gene knockdown, which likely involves proteins that regulate these pathways, such as argonaute 1 (ago1), argonaute 2 (ago2), dicer 1 (dcr1), dicer 2 (dcr2), and drosha in insects. we previously performed functional characterization of ago2 and dcr2 of western corn rootworm (wcr), diabrotica virgifera virgife ... | 2017 | 29267401 |
| iclip of the piwi protein aubergine in drosophila embryos. | piwi-interacting rnas (pirnas) are a class of small noncoding rnas bound to specific argonaute proteins, the piwi proteins. pirnas target mrnas by complementarity to silence them; they play an important role in the repression of transposable elements in the germ line of many species. pirnas and piwi proteins are also involved in diverse biological processes through their role in the regulation of cellular mrnas. in the drosophila embryo, they contribute to the maternal mrna decay occurring durin ... | 2018 | 29236253 |
| phenolics content, fruit flesh colour and browning in cultivated eggplant, wild relatives and interspecific hybrids and implications for fruit quality breeding. | increasing the content in bioactive phenolics in the eggplant (solanum melongena) fruit is of interest, but may result in enhanced browning. we evaluated six varieties of s. melongena, 22 accessions of wild related species and 42 interspecific hybrids between cultivated eggplant and wild relatives for phenolics content, fruit flesh colour, polyphenol oxidase (ppo) activity, and fruit flesh browning. wild relatives generally had a higher content in phenolics and a broader range of variation than ... | 2017 | 29195964 |
| rna-seq-derived identification of differential transcription in the eggplant (solanum melongena) following inoculation with bacterial wilt. | eggplant (solanum melongena) is a major vegetable crop worldwide. however, it is susceptible to bacterial wilt (bw) caused by ralstonia solanacearum, which has become an important factor limiting eggplant yield and quality. the underlying mechanism of bw remains unknown. here, rna-sequencing was used to characterize the transcriptomes of resistant (r) and susceptible (s) strains before (r0, s0) and after (r1, s1) r. solanacearum inoculation. after the removal of low-quality sequences and assembl ... | 2018 | 29104166 |
| pirnas and aubergine cooperate with wispy poly(a) polymerase to stabilize mrnas in the germ plasm. | piwi-interacting rnas (pirnas) and piwi proteins play a crucial role in germ cells by repressing transposable elements and regulating gene expression. in drosophila, maternal pirnas are loaded into the embryo mostly bound to the piwi protein aubergine (aub). aub targets maternal mrnas through incomplete base-pairing with pirnas and can induce their destabilization in the somatic part of the embryo. paradoxically, these aub-dependent unstable mrnas encode germ cell determinants that are selective ... | 2017 | 29101389 |
| assessment of edibility and effect of arbuscular mycorrhizal fungi on solanum melongena l. grown under heavy metal(loid) contaminated soil. | arbuscular mycorrhizal fungi (amf) aids in plant establishment at heavy metal(loid) (hm) contaminated soils, strengthening plant defense system along with promoting growth. a pot experiment was carried out to evaluate the effect of amf on eggplants grown under hm stress. further, the potential health risks of hm exposure to the humans via dietary intake of eggplant were also estimated. results showed that amf application improved growth, biomass and antioxidative defense response of plants again ... | 2018 | 29091834 |
| influence of root exudates and soil on attachment of pasteuria penetrans to meloidogyne arenaria. | the bacterium pasteuria penetrans is a parasite of root-knot nematodes (meloidogyne spp.). endospores of p. penetrans attach to the cuticle of second-stage juveniles (j2) and subsequently sterilize infected females. when encumbered by large numbers of spores, juveniles are less mobile and their ability to infect roots is reduced. this study looked at different factors that influence spore attachment of p. penetrans to the root-knot nematode meloidogyne arenaria. pretreatment of j2 with root exud ... | 2017 | 29062153 |
| cclip-seq: retrieval of chimeric reads from hits-clip (clip-seq) libraries. | hits-clip (high-throughput sequencing after in vivo crosslinking and immunoprecipitation, clip-seq) libraries contain fragments of the rna sequences bound in vivo by an rna binding protein (rbp). such fragments, especially if they represent rna duplexes bound in vivo by the rbp, can occasionally be ligated together to form chimeric clip tags. chimeric clip tags from argonaute clip libraries can provide the exact base pairing profiles of small rnas with their target rna sequences, thus solving a ... | 2018 | 29030843 |
| aubergine and pirnas promote germline stem cell self-renewal by repressing the proto-oncogene cbl. | piwi proteins play essential roles in germ cells and stem cell lineages. in drosophila, piwi is required in somatic niche cells and germline stem cells (gscs) to support gsc self-renewal and differentiation. whether and how other piwi proteins are involved in gsc biology remains unknown. here, we show that aubergine (aub), another piwi protein, is intrinsically required in gscs for their self-renewal and differentiation. aub needs to be loaded with pirnas to control gsc self-renewal and acts thr ... | 2017 | 29030484 |
| zucchini-dependent pirna processing is triggered by recruitment to the cytoplasmic processing machinery. | the pirna pathway represses transposable elements in the gonads and thereby plays a vital role in protecting the integrity of germline genomes of animals. mature pirnas are processed from longer transcripts, pirna precursors (pre-pirnas). in drosophila, processing of pre-pirnas is initiated by pirna-guided slicer cleavage or the endonuclease zucchini (zuc). as zuc does not have any sequence or structure preferences in vitro, it is not known how pirna precursors are selected and channeled into th ... | 2017 | 29021243 |
| demographic comparison of henosepilachna vigintioctopunctata (f.) (coleoptera: coccinellidae) reared on three cultivars of solanum melongena l. and a wild hostplant solanum nigrum l. | henosepilachna vigintioctopunctata (f.) is a widespread pest found on many solanaceous vegetables. the development and fecundity of h. vigintioctopunctata were studied on three cultivars ('zihong', 'eyou', and 'baijiao') of eggplant, solanum melongena l., using the two-sex life table theory. these results were then compared with similar parameters from a wild alternate host, solanum nigrum. our results showed that there were no significant differences between the developmental times of the larva ... | 2017 | 28961786 |
| green nanoparticles engineering on root-knot nematode infecting eggplants and their effect on plant dna modification. | root-knot nematodes are known to cause significant damage to eggplants. new approaches by green silver nanoparticles (gsn) are used to control plant-parasitic nematode to avoid chemical nematicide hazards. | 2016 | 28959343 |
| an in vivo proteomic analysis of the me31b interactome in drosophila germ granules. | drosophila me31b is a conserved protein of germ granules, ribonucleoprotein complexes essential for germ cell development. me31b post-transcriptionally regulates mrnas by interacting with other germ granule proteins. however, a me31b interactome is lacking. here, we use an in vivo proteomics approach to show that the me31b interactome contains polypeptides from four functional groups: rna regulatory proteins, glycolytic enzymes, cytoskeleton/motor proteins, and germ plasm components. we further ... | 2017 | 28945271 |
| combined transcriptomic and proteomic analysis constructs a new model for light-induced anthocyanin biosynthesis in eggplant (solanum melongena l.). | light is a key environmental factor affecting anthocyanin biosynthesis. our previous study demonstrated that "lanshan hexian" is a light-sensitive eggplant cultivar, but its regulatory mechanism is unknown. here, delphinidin-3-[4-(cis-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside and delphinidin-3-[4-(trans-p-coumaroyl)-rhamnosyl-glucopyranoside]-5-glucopyranoside were identified as the main anthocyanin components in lanshan hexian by ultra-performance liquid chromatography-tandem ma ... | 2017 | 28940206 |
| development and genetic characterization of advanced backcross materials and an introgression line population of solanum incanum in a s. melongena background. | advanced backcrosses (abs) and introgression lines (ils) of eggplant (solanum melongena) can speed up genetics and genomics studies and breeding in this crop. we have developed the first full set of abs and ils in eggplant using solanum incanum, a wild eggplant that has a relatively high tolerance to drought, as a donor parent. the development of these abs and il eggplant populations had a low efficiency in the early stages, because of the lack of molecular markers and genomic tools. however, th ... | 2017 | 28912788 |
| characterization and synergistic effect of antifungal volatile organic compounds emitted by the geotrichum candidum pf005, an endophytic fungus from the eggplant. | plant-associated endophytes are recognized as sources of novel bioactive molecules having diverse applications. in this study, an endophytic yeast-like fungal strain was isolated from the fruit of eggplant (solanum melongena) and identified as geotrichum candidum through phenotypic and genotypic characterizations. this endophytic g. candidum isolate pf005 was found to emit fruity scented volatiles. the compositional profiling of volatile organic compounds (vocs) revealed the presence of 3-methyl ... | 2017 | 28894891 |
| evaluation of solasonine content and expression patterns
of sgt1 gene in different tissues of two iranian eggplant (solanum melongena l.) genotypes. | eggplant (solanum melongena l.) is one of the most consumed vegetables in the world. the eggplant glycoalkaloids (gas) are toxic secondary metabolites that may have detrimental effects on human health, particularly if the magnitudes of gas are higher than the recommended food safety level (200 mg per kg of fresh mass). in this study, the content of solasonine compound and the expression patterns of solasodine galactosyltransferase (sgt1) gene were assessed in different tissues (mature leaves, fl ... | 2017 | 28867954 |
| in vitro culture responses, callus growth and organogenetic potential of brinjal (solanum melongena l.) to he-ne laser irradiation. | the present investigation was designed to analyze the influence of helium-neon (he-ne 632.8nm) laser irradiation on defense enzymes, proline content and in vitro responses of callus induction, shoot initiation and on plantlet regeneration potential of brinjal. the seeds of mattu gulla (solanum melongena l.) were irradiated with 20, 25 and 30j/cm2 of he-ne laser followed by surface sterilization and sprouted on murashige and skoog medium without plant growth regulators. the activity of defense en ... | 2017 | 28818779 |
| profiling and quantification of regioisomeric caffeoyl glucoses in solanaceae vegetables. | based on the recently developed tandem ms based hierarchical scheme for the identification of regioisomeric caffeoyl glucoses, selected vegetables were profiled with respect to their caffeoyl glucose content. the dietary plants profiled were tomato, pepper, chilli and aubergine, all members of the solanaceae family. 6-o-caffeoyl glucose was found to be the predominant isomer. in processed food such as tomato puree and ketchup a larger number of caffeoyl-glucose isomers formed through acyl migrat ... | 2017 | 28764050 |
| coding snps analysis highlights genetic relationships and evolution pattern in eggplant complexes. | brinjal (solanum melongena), scarlet (s. aethiopicum) and gboma (s. macrocarpon) eggplants are three old world domesticates. the genomic dna of a collection of accessions belonging to the three cultivated species, along with a representation of various wild relatives, was characterized for the presence of single nucleotide polymorphisms (snps) using a genotype-by-sequencing approach. a total of 210 million useful reads were produced and were successfully aligned to the reference eggplant genome ... | 2017 | 28686642 |
| crystal structure of nonspecific lipid transfer protein from solanum melongena. | lipids are considered to protect protein allergens from proteolysis and are generally seen to exist in a bound form. one of the well-known plant protein families with bound lipids is non-specific lipid transfer proteins (nsltps). structure-function relationships in the case of the members of non-specific lipid transfer protein family are not clearly understood. as part of exploring the seed proteome, we have analyzed the proteome of a member of solanaceae family, solanum melongena (eggplant) and ... | 2017 | 28612368 |
| comparative transcriptome analysis provides insights into molecular mechanisms for parthenocarpic fruit development in eggplant (solanum melongena l.). | genetic control of parthenocarpy, a desirable trait in edible fruit with hard seeds, has been extensively studied. however, the molecular mechanism of parthenocarpic fruit development in eggplant (solanum melongena l.) is still unclear. to provide insights into eggplant parthenocarpy, the transcriptomic profiles of a natural parthenocarpic (pp05) and two non-parthenocarpic (pnp05 and gnp05) eggplant lines were analyzed using rna-sequencing (rna-seq) technology. these sequences were assembled int ... | 2017 | 28604820 |
| comparative proteomic analysis of eggplant (solanum melongena l.) heterostylous pistil development. | heterostyly is a common floral polymorphism, but the proteomic basis of this trait is still largely unexplored. in this study, self- and cross-pollination of l-morph and s-morph flowers and comparison of embryo sac development in eggplant (solanum melongena l.) suggested that lower fruit set from s-morph flowers results from stigma-pollen incompatibility. to explore the molecular mechanism underlying heterostyly development, we conducted isobaric tags for relative and absolute quantification (it ... | 2017 | 28586360 |
| oxylipin mediated stress response of a miraculin-like protease inhibitor in hexanoic acid primed eggplant plants infested by colorado potato beetle. | insect-plant interactions are governed by a complex equilibrium between the mechanisms through which plant recognize insect attack and orchestrate downstream signaling events that trigger plant defense responses, and the mechanisms by which insects overcome plant defenses. due to this tight and dynamic interplay, insight into the nature of the plant defense response can be gained by analyzing changes in the insect herbivores digestive system upon plant feeding. in this work we have identified a ... | 2017 | 28578135 |
| acizzia solanicola (hemiptera: psyllidae) probing behaviour on two solanum spp. and implications for possible pathogen spread. | piercing-sucking insects are vectors of plant pathogens, and an understanding of their feeding behaviour is crucial for studies on insect population dynamics and pathogen spread. this study examines probing behaviour of the eggplant psyllid, acizzia solanicola (hemiptera: psyllidae), using the electrical penetration graph (epg) technique, on two widespread and common hosts: eggplant (solanum melongena) and tobacco bush (s. mauritianum). six epg waveforms were observed: waveform np (non-probing p ... | 2017 | 28575085 |
| aubergine controls germline stem cell self-renewal and progeny differentiation via distinct mechanisms. | piwi family protein aubergine (aub) maintains genome integrity in late germ cells of the drosophila ovary through piwi-associated rna-mediated repression of transposon activities. although it is highly expressed in germline stem cells (gscs) and early progeny, it remains unclear whether it plays any roles in early gsc lineage development. here we report that aub promotes gsc self-renewal and gsc progeny differentiation. rna-iclip results show that aub binds the mrnas encoding self-renewal and di ... | 2017 | 28441530 |
| a spiking strategy facilitates housekeeping selection for rt-qpcr analysis under different biotic stresses in eggplant. | endogenous housekeeping genes are traditionally employed to normalize the expression of target genes in rt-qpcr studies. assuming that a perfect housekeeping suitable for every condition does not exist, expression stability of the chosen reference gene should be evaluated at every new experiment. the housekeeping selection process reveals furthermore complicated and time-consuming when different conditions have to be compared in the same experimental dataset. as an alternative strategy, we spike ... | 2017 | 28429149 |
| solasodine inhibits invasion of human lung cancer cell through downregulation of mir-21 and mmps expression. | solasodine, a naturally occurring aglycone of glycoalkaloid in eggplant (solanum melongena), was found to inhibit proliferation in various tumor cells. however, the effect of solasodine on cancer cell metastasis remains unclear. this study investigates the suppression mechanism of solasodine on motility of human lung cancer cell a549 in vitro. results show that solasodine reduces viability of a549 cells. treatment with non-toxic doses of solasodine suppresses markedly cell invasion. solasodine r ... | 2017 | 28283413 |
| in vivo mapping of a dynamic ribonucleoprotein granule interactome in early drosophila embryos. | macromolecular complexes and organelles play crucial roles within cells, but their native architectures are often unknown. here, we use an evolutionarily conserved germline organelle, the germ granule, as a paradigm. in drosophila embryos, we map one of its interactomes using a novel in vivo crosslinking approach that employs two interacting granule proteins and determines their common neighbor molecules. we identified an in vivo granule assembly of tudor, aubergine, motor and metabolic proteins ... | 2016 | 28203524 |
| emerging food allergens: identification of polyphenol oxidase as an important allergen in eggplant (solanum melongena l.). | although many allergens have been detected in eggplant (solanum melongena l.), their identity have not been elucidated. the aim of this study was to investigate whether polyphenol oxidase (ppo), an important eggplant enzyme, acts as an allergen. the proteins of eggplant peel extract were separated on phenyl-sepharose (ps), and analyzed by skin prick test (spt), elisa and ige-immunoblotting; the components were analyzed for ppo activity, presence of protein-bound copper, and recognition by rabbit ... | 2017 | 28164808 |
| the effect of organic fertilizers and flowering plants on sheet-web and wolf spider populations (araneae: lycosidae and linyphiidae) and its importance for pest control. | the main goal of this study was to identify the treatment that increases the populations of spiders, which are effective predators in agroecosystems. in 2013 and 2014 the experimental eggplant (solanum melongena l.) field was two different treatments, organic fertilizers and chemical fertilizer treatment, and in 2014 we surrounded organic fertilizer plots with the flowering plants mealy cup sage (salvia farinacea benth.), spearmint (mentha spicata l.), and basil (ocimum basilicum l.). analysis u ... | 2016 | 28076280 |
| phylogenetic origin and diversification of rnai pathway genes in insects. | rna interference (rnai) refers to the set of molecular processes found in eukaryotic organisms in which small rna molecules mediate the silencing or down-regulation of target genes. in insects, rnai serves a number of functions, including regulation of endogenous genes, anti-viral defense, and defense against transposable elements. despite being well studied in model organisms, such as drosophila, the distribution of core rnai pathway genes and their evolution in insects is not well understood. ... | 2016 | 28062756 |
| effects of grilling on total polyphenol content and antioxidant capacity of eggplant (solanum melongena l.). | cooking can change the polyphenol contents of eggplant. this study elucidated the effects of grilling on total polyphenol content (tpc), antioxidant capacity, and the inner structures of eggplant. after identical hollowing, cylindrical eggplant samples were prepared and were then grilled until their center temperatures (ct) respectively reached 50, 65, 75, 85, and 95 °c. chemical assays and observations of the inner structures clarified that tpc and 1, 1-diphenyl-2-picrylhydrazyl (dpph) radical ... | 2017 | 27973738 |
| rna interference in the colorado potato beetle, leptinotarsa decemlineata: identification of key contributors. | rna interference (rnai) is a useful reverse genetics tool for investigation of gene function as well as for practical applications in many fields including medicine and agriculture. rnai works very well in coleopteran insects including the colorado potato beetle (cpb), leptinotarsa decemlineata. we used a cell line (lepd-sl1) developed from cpb to identify genes that play key roles in rnai. we screened 50 genes with potential functions in rnai by exposing lepd-sl1 cells to dsrna targeting one of ... | 2016 | 27687845 |
| violet led light enhances the recruitment of a thrip predator in open fields. | the predatory bug orius sauteri is an indigenous natural enemy of thrips and whiteflies in asian countries. to put these bugs to practical use in pest management, methods to attract and retain the bugs in agricultural fields are needed. we previously showed that violet light (405 nm) attracts o. sauteri selectively. many thrips and whiteflies are attracted to uv or green light. in this study, we examined the effect of violet-led illumination on o. sauteri in pesticide-free eggplant (solanum melo ... | 2016 | 27604315 |
| rna helicase spn-e is required to maintain aub and ago3 protein levels for pirna silencing in the germline of drosophila. | germline-specific rna helicase spindle-e (spn-e) is known to be essential for pirna silencing in drosophila that takes place mainly in the perinuclear nuage granules. loss-of-function spn-e mutations lead to tandem stellate genes derepression in the testes and retrotransposon mobilization in the ovaries. however, spn-e functions in the pirna pathway are still obscure. analysis of total library of short rnas from the testes of spn-e heterozygous flies revealed the presence of abundant pirna ping- ... | 2016 | 27320195 |
| phenotyping of eggplant wild relatives and interspecific hybrids with conventional and phenomics descriptors provides insight for their potential utilization in breeding. | eggplant (solanum melongena) is related to a large number of wild species that are a source of variation for breeding programmes, in particular for traits related to adaptation to climate change. however, wild species remain largely unexploited for eggplant breeding. detailed phenotypic characterization of wild species and their hybrids with eggplant may allow identifying promising wild species and information on the genetic control and heterosis of relevant traits. we characterizated six eggpla ... | 2016 | 27242876 |
| short-term water deficit changes cuticular sterol profile in the eggplant (solanum melongena). | crop irrigation uses a majority of a total world water supply, at the same time displaying low efficiency. as the expected, future water requirements are higher than the current ones; there is a risk of a growing deficit of water for the agricultural use. hence, there is an arising need for better understanding the effects of water deprivation on the crop plants. eggplant (solanum melongena l.) is a vegetable crop cultivated in arid and semi-arid parts of the world. because of its high water dem ... | 2016 | 27127890 |
| spindle-e cycling between nuage and cytoplasm is controlled by qin and piwi proteins. | transposable elements (tes) are silenced in germ cells by a mechanism in which piwi proteins generate and use piwi-interacting ribonucleic acid (pirna) to repress expression of te genes. pirna biogenesis occurs by an amplification cycle in microscopic organelles called nuage granules, which are localized to the outer face of the nuclear envelope. one cofactor required for amplification is the helicase spindle-e (spn-e). we found that the spn-e protein physically associates with the tudor domain ... | 2016 | 27091448 |
| novel mutants of the aubergine gene. | aubergine is an rna-binding protein of the piwi clade, functioning in germline in the pirna pathway that silences transposons and repetitive sequences. several mutations of this gene exist, but they mostly result in truncated proteins or correspond to mutations that also affect neighboring genes. we have generated complete aubergine knock-out mutants that do not disrupt the neighboring genes. these novel mutants are characterized by pcr and sequencing. their nature is confirmed by female sterili ... | 2016 | 27064345 |
| mrnas hit a sticky wicket. | drosophila germ cell specification depends on localization of mrnas required for patterning to the posterior of the oocyte during oogenesis. in a recent issue of nature, vourekas et al. (2016) suggest that aubergine in complex with pirnas may provide a low-specificity anchoring mechanism for posterior mrnas. | 2016 | 27046827 |
| crop wild relatives of the brinjal eggplant (solanum melongena): poorly represented in genebanks and many species at risk of extinction. | crop wild relatives (cwr) provide important traits for plant breeding, including pest, pathogen, and abiotic stress resistance. therefore, their conservation and future availability are essential for food security. despite this need, the world's genebanks are currently thought to conserve only a small fraction of the total diversity of cwr. | 2016 | 27026215 |
| cobalt oxide nanoparticles aggravate dna damage and cell death in eggplant via mitochondrial swelling and no signaling pathway. | despite manifold benefits of nanoparticles (nps), less information on the risks of nps to human health and environment has been studied. cobalt oxide nanoparticles (co3o4-nps) have been reported to cause toxicity in several organisms. in this study, we have investigated the role of co3o4-nps in inducing phytotoxicity, cellular dna damage and apoptosis in eggplant (solanum melongena l. cv. violetta lunga 2). to the best of our knowledge, this is the first report on co3o4-nps showing phytotoxicity ... | 2016 | 26988690 |
| mapping quantitative trait loci affecting biochemical and morphological fruit properties in eggplant (solanum melongena l.). | eggplant berries are a source of health-promoting metabolites including antioxidant and nutraceutical compounds, mainly anthocyanins and chlorogenic acid; however, they also contain some anti-nutritional compounds such as steroidal glycoalkaloids (sga) and saponins, which are responsible for the bitter taste of the flesh and with potential toxic effects on humans. up to now, quantitative trait loci (qtl) for the metabolic content are far from being characterized in eggplant, thus hampering the a ... | 2016 | 26973692 |
| solanum melongena polyphenol oxidase biosensor for the electrochemical analysis of paracetamol. | a new strategy for the construction of a polyphenol oxidase carbon paste biosensor for paracetamol detection is reported. the eggplant (solanum melongena) was processed to collect the polyphenol oxidase as an enzyme that was incorporated in the carbon paste sensor construction. the constructed sensor displayed high sensitivity and good selection for paracetamol detection and recognition. optimized conditions included ph 6.0 (highest activity), ph 7.0 (highest stability), pulse amplitude of 50 mv ... | 2016 | 26930128 |
| solanidine and tomatidine trigger scar pruritus. | scar pruritus is frequently encountered in clinical practice (particularly in burn patients) owing to its poorly known pathogenesis and difficult treatment. in previous work, we demonstrated the usefulness of a diet excluding edible solanaceae (viz., potatoes, tomatoes, peppers and aubergines) in patients with antihistamine-resistant scar pruritus. we hypothesized that alkaloids in solanaceae (particularly their secondary metabolites or aglycones) might be the actual pruritogens. in order to tes ... | 2016 | 26777454 |
| optimization of ultrasound-assisted extraction of total monomeric anthocyanin (tma) and total phenolic content (tpc) from eggplant (solanum melongena l.) peel. | the present study describes the extraction of total monomeric anthocyanin (tma) and total phenolic content (tpc) from eggplant peel using ultrasonic treatments and methanol and 2-propanol as extraction solvents. the extraction yields were optimized by varying the solvent concentration, ultrasonic frequency, temperature and time of ultrasonic treatment. box-behnken design was used to investigate the effect of process variables on the ultrasound-assisted extraction. the results showed that for tpc ... | 2016 | 26701808 |
| anther culture in eggplant (solanum melongena l.). | the technique of in vitro anther culture is the most favorite to incite the production of plants from microspore through direct embryogenesis or regeneration from callus. anther culture has been employed since 1980s in eggplant to obtain double-haploid plants from microspore derived embryos. from that time it has been refined and widely applied both at commercial level for a fast generation double-haploid parental lines of f1 hybrids, as well as for experimental studies as the complete homozygos ... | 2016 | 26619880 |
| roles of catalase (cat) and ascorbate peroxidase (apx) genes in stress response of eggplant (solanum melongena l.) against cu(+2) and zn(+2) heavy metal stresses. | eggplant (solanum melongena l.) is a good source of minerals and vitamins and this feature makes its value comparable with tomato which is economically the most important vegetable worldwide. due to its common usage as food and in medicines, eggplant cultivation has a growing reputation worldwide. but genetic yield potential of an eggplant variety is not always attained, and it is limited by some factors such as heavy metal contaminated soils in today's world. today, one of the main objectives o ... | 2015 | 26530238 |
| evaluation of suitable reference genes for normalization of qpcr gene expression studies in brinjal (solanum melongena l.) during fruit developmental stages. | brinjal/eggplant/aubergine is one of the major solanaceous vegetable crops. recent availability of genome information greatly facilitates the fundamental research on brinjal. gene expression patterns during different stages of fruit development can provide clues towards the understanding of its biological functions. quantitative real-time pcr (qpcr) has become one of the most widely used methods for rapid and accurate quantification of gene expression. however, its success depends on the use of ... | 2016 | 26472671 |
| cooking influence on physico-chemical fruit characteristics of eggplant (solanum melongena l.). | physico-chemical traits of three eggplant genotypes ("tunisina", "buia" and "l 305") were evaluated before and after two cooking treatments (grilling and boiling). different genotypes revealed different changes after cooking, with "tunisina" showing a better retention of phytochemicals with respect to other two genotypes. the main physical phenomena were water loss during grilling, and dry matter loss after boiling. chlorogenic acid, the main phenolic in eggplant, resulted higher in grilled samp ... | 2016 | 26471625 |
| ige response to two new allergen proteins of solanum melongena l. (eggplant). | a number of allergens from eggplant (solanum melongena l.) have been previously identified. in this study, we could detect ige reactivity of two allergic subjects' sera towards two protein bands of molecular mass of about 35 and 15 kda. as ige were reactive to both raw and cooked eggplant extracts, a heat-stable nature of these novel allergens is apparent. | 2015 | 26455782 |
| signs, symptoms and treatment of penile fracture. | penile fracture is an uncommon injury and requires urgent treatment, therefore emergency nurses should be aware of the signs and symptoms and understand the importance of immediate surgical referral. this article describes the anatomy and physiology of penile erection and the ways in which penile fracture can occur. it also outlines the management of patients and includes a case study of a fracture caused by vigorous masturbation. | 2015 | 26451939 |
| a new γ-alkylated-γ-butyrolactone from the roots of solanum melongena. | a new γ-alkylated-γ-butyrolactone, named melongenolide a (1), along with nine known compounds were obtained from the roots of solanum melongena, and their structures were identified as melongenolide a (1), (+)-syringaresinol (2), (+)-lyoniresinol (3), 5,5'-dimethoxy lariciresinol (4), (+)-(7r,8r)-4-hydroxy-3,3',5'-trimethoxy-8',9'-dinor-8,4'-oxyneoligna-7, 9-diol-7'-aldehyde (5), kaempferol-3-o-(2″,6″-di-o-p-trans-coumaroyl)-β-glucoside (6), arjunolic acid (7), vanillic acid (8), scoparone (9), ... | 2015 | 26412430 |
| long-distance transport of cadmium from roots to leaves of solanum melongena. | in this study, the characteristics of cadmium (cd) uptake by roots and translocation from roots to leaves of two eggplant species (solanum melongena and solanum torvum) under relatively low cd concentrations were investigated using stable (108)cd isotope through a number of hydroponic experiments. the uptake and translocation of (108)cd was compared with those of (70)zn and (15)n. the results showed more (108)cd was loaded to the vascular channels and translocated upward to the leaves in s. melo ... | 2015 | 26407708 |
| electrochromic properties of acid dye doped poly(3,4-ethylenedioxythiophene) by electropolymerization. | the electrochromic properties of an electrochemical polymerized composite consisted of poly(3,4-ethylenedioxythiophene) doped with acid red g (pedot-arg) are reported. the structures of pedot-arg were characterized via cycle voltammograms, spectroelectrochemistry and colorimetric analysis. film of the pedot-arg composites switches from aubergine in the reduction state to light blue in the oxidized state, which is different from the pure pedot of dark blue (-0.8 v) and light blue (1.0 v). because ... | 2015 | 26353549 |
| dioscin derived from solanum melongena l. "usukawamarunasu" attenuates α-msh-induced melanogenesis in b16 murine melanoma cells via downregulation of phospho-creb and mitf. | this study aimed to chemically isolate and explore an antimelanogenesis inducer in extracts of solanum melongena l. "usukawamarunasu" eggplant. we successfully identified dioscin ([25r]-spirost-5-en-3β-yl) 2-o-(6-deoxy-α-l-mannopyranosyl) - 4-o-(6-deoxy-α-l-mannopyranosyl)-β-d-glucopyranoside] in the plant, and examined the effects of α-melanocyte-stimulating hormone (msh)-induced melanogenesis in b16 murine melanoma cells by this plant-derived dioscin. immunoblot analysis suggested that dioscin ... | 2015 | 26352003 |
| aub and ago3 are recruited to nuage through two mechanisms to form a ping-pong complex assembled by krimper. | in drosophila, two piwi proteins, aubergine (aub) and argonaute-3 (ago3), localize to perinuclear "nuage" granules and use guide pirnas to target and destroy transposable element transcripts. we find that aub and ago3 are recruited to nuage by two different mechanisms. aub requires a pirna guide for nuage recruitment, indicating that its localization depends on recognition of rna targets. ago3 is recruited to nuage independently of a pirna cargo and relies on interaction with krimper, a stable c ... | 2015 | 26295961 |
| association mapping for fruit, plant and leaf morphology traits in eggplant. | an eggplant (solanum melongena) association panel of 191 accessions, comprising a mixture of breeding lines, old varieties and landrace selections was snp genotyped and phenotyped for key breeding fruit and plant traits at two locations over two seasons. a genome-wide association (gwa) analysis was performed using the mixed linear model, which takes into account both a kinship matrix and the sub-population membership of the accessions. overall, 194 phenotype/genotype associations were uncovered, ... | 2015 | 26284782 |
| aubergine iclip reveals pirna-dependent decay of mrnas involved in germ cell development in the early embryo. | the piwi-interacting rna (pirna) pathway plays an essential role in the repression of transposons in the germline. other functions of pirnas such as post-transcriptional regulation of mrnas are now emerging. here, we perform iclip with the piwi protein aubergine (aub) and identify hundreds of maternal mrnas interacting with aub in the early drosophila embryo. gene expression profiling reveals that a proportion of these mrnas undergo aub-dependent destabilization during the maternal-to-zygotic tr ... | 2015 | 26257181 |
| krimper enforces an antisense bias on pirna pools by binding ago3 in the drosophila germline. | piwi-interacting rnas (pirnas) suppress transposon activity in animal germ cells. in the drosophila ovary, primary aubergine (aub)-bound antisense pirnas initiate the ping-pong cycle to produce secondary ago3-bound sense pirnas. this increases the number of secondary aub-bound antisense pirnas that can act to destroy transposon mrnas. here we show that krimper (krimp), a tudor-domain protein, directly interacts with pirna-free ago3 to promote symmetrical dimethylarginine (sdma) modification, ens ... | 2015 | 26212455 |
| top-down expectancy versus bottom-up guidance in search for known color-form conjunctions. | we assessed the effects of pairing a target object with its familiar color on eye movements in visual search, under conditions where the familiar color could or could not be predicted. in experiment 1 participants searched for a yellow- or purple-colored corn target amongst aubergine distractors, half of which were yellow and half purple. search was more efficient when the color of the target was familiar and early eye movements more likely to be directed to targets carrying a familiar color tha ... | 2015 | 26198709 |
| isolation and immobilization of alkaline protease on mesoporous silica and mesoporous zsm-5 zeolite materials for improved catalytic properties. | alkaline protease from brinjal leaf (solanum melongena) having milk clotting activity has been purified to 9.44 fold to a final specific activity of 45.71 u/mg. sds-page of the final preparation revealed a single protein band of approx 14 kda. purified enzyme was characterized and was successfully immobilized into the amorphous mesoporous silica (sba-15) and crystalline mesoporous zeolite (nano-zsm-5) using entrapment method. maximum immobilization of 63.5% and 79.77% was obtained with sba-15 an ... | 2015 | 29124151 |
| the drosophila fragile x mental retardation protein participates in the pirna pathway. | rna metabolism controls multiple biological processes, and a specific class of small rnas, called pirnas, act as genome guardians by silencing the expression of transposons and repetitive sequences in the gonads. defects in the pirna pathway affect genome integrity and fertility. the possible implications in physiopathological mechanisms of human diseases have made the pirna pathway the object of intense investigation, and recent work suggests that there is a role for this pathway in somatic pro ... | 2015 | 25908854 |
| exogenous proline application ameliorates toxic effects of arsenate in solanum melongena l. seedlings. | hydroponic experiments were conducted to investigate an effect of exogenous application of proline (pro; 25 µm) in alleviating arsenate (as(v); 5 and 25 µm) toxicity in solanum melongena l. (eggplant) seedlings. exposure of as(v) declined growth of eggplant, which was coincided with an enhanced accumulation of as. however, exogenous pro application alleviated as(v) toxicity in eggplant seedlings by reducing the accumulation of as. the fluorescence characteristics (jip-test): φp0, ψ0, φe0, piabs, ... | 2015 | 25881134 |
| parallel reductions in phenolic constituents resulting from the domestication of eggplant. | crop domestication is often accompanied by changes in metabolite compositions that alter traits such as flavor, color, or other beneficial properties. fruits of eggplants (solanum melongena l.) and related species are abundant and diverse in pharmacologically interesting phenolic compounds, particularly hydroxycinnamic acid (hca) conjugates such as the antioxidant caffeoylquinic acids (cqa) and hca-polyamine amides (hcaa). to understand metabolite variability through the lens of natural and arti ... | 2015 | 25813879 |
| characterization and quantitative analysis of phenylpropanoid amides in eggplant (solanum melongena l.) by high performance liquid chromatography coupled with diode array detection and hybrid ion trap time-of-flight mass spectrometry. | eggplant (solanum melongena l.) is a famous edible and medicinal plant. despite being widely cultivated and used, data on certain parts other than the fruit are limited. the present study focused on the qualitative and quantitative analysis of the chemical constituents, particularly phenylpropanoid amides (pas), in eggplant. the mass fragmentation patterns of pas were proposed using seven authentic compounds with the assistance of a hybrid ion trap time-of-flight mass spectrometer. thirty-seven ... | 2015 | 25796999 |