| histochemical analysis of rat testicular glycoconjugates. 3. non-reducing terminal residues in seminiferous tubules. | lectins of helix pomatia (hpa), glycine max (sba), vicia villosa (vva), dolichos biflorus (dba), ulex europaeus (uea-1), tetragonolobus purpureus (lta), griffonia simplicifolia (bsa-1b4), maclura pomifera (mpa), sambucus nigra (sna) and maackia amurensis (maa) were used to explore the distribution of saccharides characteristic of non-reducing termini of o- and n-linked glycoprotein glycans in the seminiferous tubules of rat testis. sialyl residues (both alpha 2,3- and alpha 2,6-linked, as shown ... | 1993 | 8282567 |
| effects of extrusion and expelling on the nutritional quality of conventional and kunitz trypsin inhibitor-free soybeans. | two chick growth experiments and a precision-fed cockerel digestibility assay were conducted to evaluate the effect of extrusion and expelling on the nutritive value of conventional (csb) and kunitz trypsin inhibitor-free (kfsb) soybeans. in the first experiment, performance of chicks fed csb or kfsb autoclaved at 121 c was similar to that of chicks fed csb or kfsb extruded at 138 c. the effect of extrusion temperature on protein quality of the soybeans was evaluated in the second experiment. el ... | 1993 | 8309879 |
| lectin histochemistry of normal and neoplastic peripheral nerve sheath. 1. lectin binding pattern of normal peripheral nerve in man. | the binding patterns of lectins to normal peripheral nerves were examined. twelve biotinylated lectins were used in this study; canavalia ensiformis (con a), pisum sativum (psa), lens culinaris (lca), ricinus communis 1 (rca-1), arachis hypogaea (pna), glycine max (sba), sophora japonica (sja), bandeiraea simplicifolia 1 (bsl-1), triticum vulgaris (wga), succinylated wga (s-wga), ulex europaeus 1 (uea-1) and helix pomatia (hpa). cytoplasm of schwann cells and perineurial cells was stained by con ... | 1993 | 8310810 |
| molecular analysis of avirulence gene avrrpt2 and identification of a putative regulatory sequence common to all known pseudomonas syringae avirulence genes. | the avrrpt2 locus from pseudomonas syringae pv. tomato causes virulent strains of p. syringae to be avirulent on some, but not all, lines of arabidopsis thaliana and glycine max (soybean). we determined the dna sequence of the avrrpt2 locus and identified the avrrpt2 gene as a 768-bp open reading frame encoding a putative 28.2-kda protein. deletion analysis and transcription studies provided further evidence that this open reading frame encodes avrrpt2. we found that the avrrpt2 gene also has av ... | 1993 | 8335641 |
| changes of lectin staining pattern of the golgi stack during differentiation of the ameloblast in developing rat molar tooth germs. | changes of lectin staining patterns in the golgi stack during cell differentiation were examined in the ameloblasts of developing rat molar tooth germs, using hrp-labeled lectins: canavalia ensiformis (con a), griffonia simplicifolia i (gs-i), glycine max (sba), ulex europeus i (uea-i), triticum vulgaris (wga), and arachis hypogaea (pna). the golgi stacks of the inner enamel epithelial cells and the presecretory ameloblasts were stained with the lectins, although the staining strength and patter ... | 1993 | 8338238 |
| a high-order structure of plant storage proprotein allows its second conversion by an asparagine-specific cysteine protease, a novel proteolytic enzyme. | during seed embryogenesis, glycinin, the 11-s seed storage protein found in soybeans, undergoes post-translational proteolytic processing, in which a proprotein molecule is cleaved into an acidic and a basic subunit by a one-point cleavage that occurs at the carboxyl side of the asparaginyl residue located at the junction of the subunits. to elucidate the mechanism of this very limited proteolysis, we purified the cysteine endoprotease and used purified proglycinin produced by escherichia coli a ... | 1993 | 8344272 |
| preparation and production of a cancer chemopreventive agent, bowman-birk inhibitor concentrate. | we describe our studies to produce an extract of soybeans with anticarcinogenic activity that we believe will be useful as a human cancer chemopreventive agent for several different organs. the anticarcinogenic activity of the extract is thought to be due to chymotrypsin inhibitor activity, which is due to the bowman-birk protease inhibitor (bbi) present in the extract, termed bbi concentrate (bbic). we describe the contents of bbic, the ability of bbic to inhibit malignant transformation in vit ... | 1993 | 8346077 |
| the structures and biological activities of the lipo-oligosaccharide nodulation signals produced by type i and ii strains of bradyrhizobium japonicum. | bradyrhizobium japonicum produces lipo-oligosaccharide signal molecules that induce deformation of root hairs and meristematic activity on soybeans. b. japonicum usda135 (a type i strain) produces modified chitin pentasaccharide molecules with either a terminal n-c16:0- or n-c18:1-glucosamine with and without an o-acetyl group at c-6 and with 2-o-methylfucose linked to c-6 of the reducing n-acetylglucosamine. an additional molecule has n-c16:1-glucosamine and no o-acetyl group. all of these mole ... | 1993 | 8349712 |
| comparative sequence analysis of cis elements present in glycine max l. leghemoglobin lba and lbc3 genes. | the soybean leghemoglobin lba gene promoter sequence was determined and aligned with the promoter sequence of the soybean lbc3 gene from the same gene family. five highly conserved regions were found. there are two large conserved regions, one of which overlaps the basic promoter while the other defines a minimal enhancer in the upstream positive elements. within the minimal enhancer, an inverted repeat with similarity to the binding site of a yeast transcription factor, gcn4, was found. this pa ... | 1993 | 8358040 |
| the in vitro destruction of rumen fluid carotenoids by plant lipoxygenases. | an ability to destroy carotenoids in the rumen of cattle may be an effective method of limiting their absorption from the small intestine which, in turn, is likely to result in reduced adipose tissue colour. plant lipoxygenases are well known for their ability to bleach beta-carotene. as lutein is the major carotenoid present in most forage grasses, we have investigated the bleaching of lutein as well as beta-carotene by lipoxygenase isolated from soybeans. in vitro studies, using micellar prepa ... | 1993 | 8364403 |
| sensory evaluation and nutrient composition of weaning food from pregelatinized maize-sweet potato mixtures. | pregelatinized maize-sweet potato mix, fortified with soybeans and groundnut flours, was evaluated for its acceptability as a weaning food and for nutrient composition. an acceptable product had 14.3, 6.4, 2.4 and 67.4%, respectively, for its protein, fat, ash and carbohydrate contents. values for calcium, phosphorus and and iron contents were 59.6, 187.5 and 2.4 mg/100 g, respectively, while the most predominant fatty acids were linoleic, oleic and palmitic acids. the product, which had a chemi ... | 1993 | 8378272 |
| comparison of whole, raw soybeans, extruded soybeans, or soybean meal and barley on digestive characteristics and performance of weaned beef steers consuming mature grass hay. | two experiments were conducted to compare whole, raw soybeans (wsb), extruded soybeans (esb), and soybean meal+barley (sbm+bar) as supplemental protein sources for growing beef steers consuming low-quality, mature grass hay (6.5% cp). in exp. 1, a 23-d digestion study, four ruminally cannulated steers were assigned to the following treatments in a 4 x 4 latin square design: 1) control, no supplement; 2) 1.5 kg.animal-1.d-1 of wsb; 3) 1.36 kg.animal-1.d-1 of esb; and 4) 1.48 kg.animal-1.d-1 of 62 ... | 1993 | 8384196 |
| glycan localization within the human interphotoreceptor matrix and photoreceptor inner and outer segments. | glycoconjugates are likely to be of fundamental importance in the complex interactions between photoreceptors and the retinal pigment epithelium, but few have been characterized, especially in human tissue. as a preliminary step towards determining their biological functions in health and disease, a lectin-based histochemical study of the glycan expression of human outer retina was performed on glutaraldehyde-fixed, semi-thin, resin-embedded sections. the interphotoreceptor matrix and photorecep ... | 1993 | 8400552 |
| supplemental dietary fat from extruded soybeans and calcium soaps of fatty acids for lactating cows. | holstein cows (n = 33) were assigned to one of three diets containing soybean meal, supplemental fat from extruded soybeans, or supplemental fat from ca soaps of fatty acids. total mixed diets containing (dm basis) 25% corn silage, 25% alfalfa hay, and 50% of the respective concentrate mixtures were fed wk 4 through 15 postpartum. milk production (29.2, 32.4, and 31.8 kg/d) was higher for cows fed supplemental fat diets. milk protein percentages (2.99, 2.93, and 2.81) were higher for cows fed th ... | 1993 | 8436673 |
| isolation and characterization of a beta-tubulin-encoding gene from colletotrichum gloeosporioides f. sp. aeschynomene. | colletotrichum gloeosporioides f. sp. aeschynomene (c.g.a.) is a fungal pathogen of legumes and is used as a commercial mycoherbicide for rice and soybeans. as an initial study to potentially improve the utility of this fungus and develop a gene transfer system, a beta-tubulin (beta tub)-encoding gene (tub1) was isolated, cloned and sequenced. the coding sequence and deduced amino acid sequence of the c.g.a. tub1 gene was highly homologous to the tub1 gene of colletotrichum graminicola. southern ... | 1993 | 8440473 |
| plasma membrane alterations of maturing goat (capra indicus) spermatozoa: lectin-binding and freeze-fracture study. | a qualitative and quantitative analysis of lectin-binding sites has been undertaken on spermatozoa recovered from different regions of the epididymis of the goat (capra indicus) using fluorescein isothiocyanate-linked lectins (bauhinia purpurea bpa, concanavalin a con a, dolichos biflorus dba, maclura pomifera mpa, arachis hypogaea or peanut agglutinin pna, glycine max or soyabean agglutinin sba, ulex europaeus uea, and triticum vulgaris or wheat-germ agglutinin wga), in conjunction with scannin ... | 1993 | 8443831 |
| isolation and characterization of a cdna encoding nadp(+)-specific isocitrate dehydrogenase from soybean (glycine max). | a cdna that encodes an nadp-specific isocitrate dehydrogenase (idh) was cloned from a soybean nodule cdna library by complementation of an escherichia coli mutant that lacked idh. dna sequence analysis showed that the 1583 bp soybean cdna could encode a protein that shares 63.9% amino acid sequence identity with the saccharomyces cerevisiae nadp-idh and long sequences of identity to an idh from pig. southern blot analysis suggests that this gene corresponds to a gene family made up of no more th ... | 1993 | 8467073 |
| serum enzymes as indicators of capture myopathy in mallards (anas platyrhynchos). | serum concentrations of the enzymes creatine kinase (ck) and aspartate aminotransferase (ast) were measured in captive and wild mallards (anas platyrhynchos) as indicators of muscle damage. baseline values for both enzymes were determined from six captive male mallards. during winter 1990 to 1991, six diets (including controls) representative of food available in the mississippi alluvial valley were fed to captive female mallards housed in an outdoor aviary at the white river national wildlife r ... | 1993 | 8487381 |
| [trimethylaminuria: fish-odor syndrome]. | fish-odor syndrome is due to a rare metabolic defect in which there is massive excretion of the volatile tertiary amine, trimethylamine (tma) in the urine. the typical fish-like odor of tma also appears on the breath or in the sweat of affected individuals. in normal man tma is oxidized to its odorless n-oxide derivative. tma is of dietary origin, formed by intestinal bacterial degradation of the choline in egg yolk, liver, soybeans, etc., or by reduction of tma-oxide present in high concentrati ... | 1993 | 8495879 |
| direct end labelling of telomeres. | a novel approach of direct end labelling of telomeres is presented. chromosome-sized, agarose-embedded dna was treated with t4 dna polymerase to remove protruding 3' end of telomeres and to generate single-stranded 5' ends. the dna was then labelled by the same enzyme in the presence of [alpha-32p]dgtp and cold datp and dttp. labelled yeast chromosomes separated by pulsed field gel electrophoresis maintained their integrity. digestion of yeast chromosomes separated in pulsed field gels with a re ... | 1993 | 8514152 |
| capacitated and acrosome reacted spermatozoa of goat (capra indicus): a fluorescence and electron microscopic study. | plasma membrane alterations accompanying in vitro capacitation and acrosome reaction of goat spermatozoa were studied using lectin labelling, scanning electron microscopy, and freeze-fracture methods. fluorescein isothiocyanate linked lectins namely; canavalia ensiformis (cona), maclura pomifera (mpa), arachis hypogaea (pna), glycine max (sba) and triticum vulgaris (wga) agglutinin were used to examine the distribution of surface carbohydrates during these two events. the head and the sperm tail ... | 1993 | 8517552 |
| neurons with perineuronal sulfated proteoglycans in the human visual cortex, with special reference to their reactions to lectins. | the human visual cortex, especially its ganglionic lamina, was found to contain many neurons with perineuronal sulfated proteoglycans which were stained with cationic iron colloid and aldehyde fuchsin. it also contained many neurons with surface glycoproteins labeled with lectin vicia villosa agglutinin (vva) or glycine max agglutinin (sba). double staining frequently showed that the neurons stained with cationic iron colloid were not labeled with lectin vva or sba. hyaluronidase and chondroitin ... | 1995 | 8527242 |
| carbohydrate and peptide antigens in macrophage populations derived from human bone marrow and milk: an immunomorphological and immunochemical analysis. | an immunomorphological and immunochemical study was performed to elucidate the pattern of carbohydrate antigens and their relationships to the cluster differentiation (cd) 68 epitopes on macrophages derived from human bone marrow and milk. core and backbone antigens recognized by lectins from bauhinia purpurea (bpa), helix pomatia (hpa), arachis hypogaea (pna), glycine max. (sba), griffonia simplicifolia (gsa-i-b4), lycopersicon esculentum (lea) and erythrina cristagalli (eca) were expressed by ... | 1995 | 8550384 |
| supplemental niacin and heat-treated whole soybeans for jersey cows during early lactation. | fifty-six multiparous jersey cows were used in a replicated trial to investigate the effect of supplemental niacin and heat-treated whole soybeans on milk production and composition. dietary treatments included 0 or 6 g/d of supplemental niacin beginning 21 d prior to expected parturition and raw or heat-treated whole soybeans fed at 10% of the dietary dm beginning immediately after parturition. data were collected for 18 wk following a 2-wk postpartum period during which cows were adjusted to e ... | 1995 | 8550911 |
| a structural model for the mechanisms of elicitor release from fungal cell walls by plant beta-1,3-endoglucanase. | the release of elicitor-active carbohydrates from fungal cell walls by beta-1,3-endoglucanase contained in host tissues has been implicated as one of the earliest processes in the interaction between soybean (glycine max) and the fungal pathogen phytophthora megasperma f. sp. glycinea leading to host defense responses such as phytoalexin production. the present study was conducted to evaluate the primary structure of the glucanase-released elicitor (re). gel-filtration chromatography of carbohyd ... | 1995 | 8552716 |
| purified ferritin and soybean meal can be sources of iron for treating iron deficiency in rats. | ferritin and soybean meal were reevaluated as dietary treatments of iron deficiency in rats. isotopes that had been used in the past were avoided because of contemporary knowledge of the physiological and structural complexity of ferritin protein and the solid iron mineral. rats made anemic by iron-deficient diets were given equivalent amounts of iron as feso4, horse spleen ferritin, baked soybean meal, or soybean meal plus ferritin. full recovery (89-109%) from anemia and increased tissue iron ... | 1996 | 8558296 |
| soybean isoflavones improve cardiovascular risk factors without affecting the reproductive system of peripubertal rhesus monkeys. | although the beneficial effects of dietary soybean protein compared with animal proteins on plasma lipids, lipoproteins and atherosclerosis have been known for about 50 years, it has been uncertain whether these effects are due to its amino acid concentrations or other components in soybeans. to assess the effect of soybean protein's alcohol-extractable components (including the isoflavonic phytoestrogens genistein and daidzein) on plasma lipid and lipoprotein concentrations and to establish its ... | 1996 | 8558324 |
| degradation of stachyose, raffinose, melibiose and sucrose by different tempe-producing rhizopus fungi. | forty-six strains of tempe-forming rhizopus species were screened for their ability to grow on raffinose as the sole carbon source. six of the strains showed good growth and sporulation. these isolates were one rhizopus oligosporus, one rhizopus microsporus var. chinensis, three rhizopus oryzae and one rhizopus stolonifer. these six moulds and r. oligosporus strain nrrl 2710 were investigated for their metabolism of the raffinose family of alpha-galactoside carbohydrates. degradation experiments ... | 1995 | 8579835 |
| the alternative oxidase is encoded in a multigene family in soybean. | the copy number of the alternative oxidase gene, aox, was investigated in soybean (glycine max l.) using a polymerase chain reaction (pcr) approach to amplify fragments from soybean genomic dna. the primers used were based on absolutely conserved regions of aox cdna clones from a variety of plant species and the yeast hansenula anomala. after subcloning of the 170-bp pcr products, 12 individual colonies were sequenced. eleven plasmids yielded inserts representing three sequences in the ratio 4:3 ... | 1996 | 8580775 |
| developmental and growth temperature regulation of two different microsomal omega-6 desaturase genes in soybeans. | the polyunsaturated fatty acid content is one of the major factors influencing the quality of vegetable oils. edible oils rich in monounsaturated fatty acid provide improved oil stability, flavor, and nutrition for human and animal consumption. in plants, the microsomal omega-6 desaturase-catalyzed pathway is the primary route of production of polyunsaturated lipids. we report the isolation of two different cdna sequences, fad2-1 and fad2-2, encoding microsomal omega-6 desaturase in soybeans and ... | 1996 | 8587990 |
| site of digestion and milk production by cows fed fats differing in saturation, esterification, and chain length. | four cannulated cows in a 4 x 4 latin square design were used to study the effects of supplemental dietary fatty acids from roasted soybeans combined with tallow or partially hydrogenated fats, varying in esterification and fatty acid chain length, on nutrient digestion and lactation performance. diets were formulated to contain (dm basis) 48% forage in addition to 1.5% fatty acids from roasted soybeans and 2.5% fatty acids from tallow, partially hydrogenated tallow triglycerides, partially hydr ... | 1995 | 8598409 |
| the feeding value of soybeans fed to rats, chickens, catfish and dairy cattle is not altered by genetic incorporation of glyphosate tolerance. | animal feeding studies were conducted with rats, broiler chickens, catfish and dairy cows as part of a safety assessment program for a soybean variety genetically modified to tolerate in-season application of glyphosate. these studies were designed to compare the feeding value (wholesomeness) of two lines of glyphosate-tolerant soybeans (gts) to the feeding value of the parental cultivar from which they were derived. processed gts meal was incorporated into the diets at the same concentrations a ... | 1996 | 8598557 |
| the expressed protein in glyphosate-tolerant soybean, 5-enolpyruvylshikimate-3-phosphate synthase from agrobacterium sp. strain cp4, is rapidly digested in vitro and is not toxic to acutely gavaged mice. | the safety of 5-enolpyruvylshikimate-3-phosphate synthase enzyme derived from agrobacterium sp. strain cp4 (cp4 epsps) was assessed. cp4 epsps is the only protein introduced by genetic manipulation that is expressed in glyphosate-tolerant soybeans, which are being developed to provide new weed-control options for farmers. expression of this protein in plants imparts high levels of glyphosate tolerance. the safety of cp4 epsps was ascertained by evaluating both physical and functional characteris ... | 1996 | 8598558 |
| one-step purification of the beta-glucan elicitor-binding protein from soybean (glycine max l.) roots and characterization of an anti-peptide antiserum. | a low abundance beta-glucan elicitor-binding protein from soybean was isolated by a rapid, simple and one-step purification method yielding about 9000-fold enrichment. the affinity-based purification technique was more efficient than a procedure that uses conventional methods and preserved the binding activity to a much larger extent. the final preparation consisted of one major protein with an apparent molecular mass of about 75 kda. electrophoretic analyses of the purified and photoaffinity-la ... | 1996 | 8601456 |
| interaction of a human blood group sd(a-) tamm-horsfall glycoprotein with applied lectins. | unlike the human blood group sd(a+) tamm-horsfall glycoprotein (thgp), the sd(a-) one lacks terminal galnacbeta1--> residues at the nonreducing ends. the binding properties of this glycoprotein and its asialo product with lectins were characterized by quantitative precipitin (qpa) and precipitin inhibition assays. among 20 lectins tested by qpa, both native and asialo sd(a-) thgp reacted best with abrus precatorius and ricinus communis and completely precipitated the lectin added. they also prec ... | 1996 | 8617360 |
| comparison of the profiles of seedborne fungi and the occurrence of aflatoxins in mould-damaged beans and soybeans. | the average percentage of fungal infection in twenty bean and soybean lots was determined by visual inspection (24.5 and 16.8%, respectively), and by laboratory isolation (58.5 and 33.7%), respectively. the mycoflora were more diverse in bean than soybean. bean was more susceptible to fungal infection than soybean under similar environmental and cultural conditions. the most frequently isolated fungal genera for bean and soybean were aspergillus, penicillium, rhizopus, eurotium and curvularia. a ... | 1995 | 8628121 |
| early detection of specific ige antibody against house dust mite in children at risk of allergic disease. | house dust mite (hdm) is a representative inhalant allergen that triggers allergic disease in childhood. the aim of this study is early detection of hdm-specific ige antibody and prediction of the risk of a positive reaction to this antibody by in vitro parameters in infants with allergic manifestations. | 1996 | 8648544 |
| lectin histochemistry of pregnant rat uterine tissues. | glycoconjugate residues were examined in the rat uterus at days 10, 12 and 15 of pregnancy using 17 biotinylated lectins with specificities for a variety of carbohydrate moieties. a wide variety of glycoconjugate residues were detected in the cytoplasm of some antimesometrial and mesometrial decidual cells with lectins from canavalia ensiformis (con a), lens culinaris (lch) culinaris (lch), pisum sativum (psa), griffonia simplicifolia ii (gs-ii), triticum vulgaris (wga), griffonia simplicifolia ... | 1996 | 8655407 |
| daidzein and genistein concentrations in human milk after soy consumption. | soy isoflavones were quantified from human milk by a fast, precise, and selective hplc method after enzymatic hydrolysis of conjugated isoflavones and extraction with ethyl acetate. isoflavone aglycones and their mammalian metabolites equol and o-desmethylangolensin were separated selectively and identified by absorbance patterns, fluorometric and electrochemical detection, gas chromatography-mass spectrometric analysis after trimethylsilylation, and with internal and external authentic standard ... | 1996 | 8665689 |
| analysis of lectin- and snail plasma-binding glycopeptides associated with the tegumental surface of the primary sporocysts of schistosoma mansoni. | carbohydrates associated with the tegumental surface of schistosoma mansoni primary sporocyst may serve as potential receptors for mediating recognition by the internal defence system of the molluscan host, biomphalaria glabrata. therefore, a combination of sds-page and lectin probe analyses were carried out on biotin-labelled tegumental glycopeptides as a first step to defining the carbohydrates expressed at the sporocyst surface. the majority of surface polypeptides, ranging in relative molecu ... | 1996 | 8677136 |
| selenite incubated with nadph and mammalian thioredoxin reductase yields selenide, which inhibits lipoxygenase and changes the electron spin resonance spectrum of the active site iron. | selenite and selenodiglutathione (gs-se-sg) efficiently inhibited 5-lipoxygenase activity in sonicates of human monoclonal b-lymphocytes. the apparent ic50 of gs-se-sg was 0.5 microm. the inhibitory effect of these compounds was observed within 10 min of incubation. in order to elucidate if the mechanism of inhibition by these compounds was result of direct interference with lipoxygenase or indirectly mediated by cellular factors, pure 15-lipoxygenase from soybeans was used as a model system for ... | 1996 | 8679612 |
| an acidic amino acid-specific protease from germinating soybeans. | the degradation of the beta-conglycinin protein reserves in soybean seeds during germination and early growth begins with the proteolysis of its alpha and alpha' subunits by an enzyme called protease c1. in the pathway, a number of proteolytic intermediates are produced and subsequently degraded. determination of the n-terminal sequences of these intermediates provides insight regarding the requirements of the cleavage sites. the n-terminal sequence of three such proteolytic intermediates has be ... | 1996 | 8688170 |
| ruminal availabilities of protein and starch: effects on growth and ruminal and plasma metabolites of dairy calves. | newborn holstein calves (n = 75) were blocked by date of birth and sex and assigned randomly to one of eight isonitrogenous starters that contained protein and starch sources of different ruminal availabilities. soybean meal or soybeans roasted to an exit temperature of 146 degrees c, raw or conglomerated corn, and urea at 1% of dm or no urea were used in a 2 x 2 x 2 factorial arrangement. the conglomeration process consisted of grinding the grain, adding water, pelleting the mixture, and roasti ... | 1996 | 8708089 |
| effect of roasted soybeans and corn on performance and ruminal and blood metabolites of dairy calves. | newborn holstein calves (n = 132) were used to evaluate three protein sources (soybean meal and soybeans roasted to an exit temperature of 138 or 146 degrees c) that contained 30, 45, or 52% rup, respectively. each was used with either raw corn or corn roasted to an exit temperature of 135 degrees c. roasting of corn increased starch gelatinization from 34.8 to 118.6 mg of maltose equivalents/g of sample. six pelleted isonitrogenous calf starters (18% cp) were fed for ad libitum consumption from ... | 1996 | 8708109 |
| biochemical features of grain legume allergens in humans and animals. | peanuts and soybeans are the major legumes involved in human food allergy, although some data exist on adverse reactions to temperate legumes including pea, green bean, sweet lupin, and lentil. an increasing number of legume proteins or glycoproteins have been characterized as food allergens. limited data tend to indicate that they are usually different from legume inhalent allergens. cross-recognition among legume allergens is immunochemically frequent but clinically less common. a common featu ... | 1996 | 8710238 |
| plant clathrin heavy chain: sequence analysis and restricted localisation in growing pollen tubes. | clathrin-coated vesicles were isolated from soybean (glycine max l.) cells in suspension culture and their purity was assessed using sds-page, peptide sequencing and electron microscopy. antibodies raised to these coated vesicles were used to immunoscreen a soybean cdna library in lambda gt11 and isolate a partial clone of the clathrin heavy chain (hc) gene. full-length cdna for soybean clathrin hc was deduced by 5' and 3' cdna amplification. the cdna encodes an amino acid sequence of 1,700 resi ... | 1996 | 8718669 |
| effects of dehulling, cooking and storage conditions on protein quality and digestibility of soybeans. | soybeans were dehulled, stored under two environmental conditions [25 degrees c/75% r.h. (env. 1) and 38 degrees c/90% r.h. (env.2)], optimally cooked and assayed for trypsin inhibitor and protein quality with laboratory rats. dehulling did not significantly affect protein quality (per and npr) and protein digestibility of raw and cooked soybeans. raw soybeans diets were significantly poorer in protein quality and digestibility when compared with cooked counterparts. per values of dehulled-cooke ... | 1995 | 8729251 |
| beta-glucan synthesis in bradyrhizobium japonicum: characterization of a new locus (ndvc) influencing beta-(1-->6) linkages. | bradyrhizobium japonicum synthesizes periplasmic cyclic beta-(1-->3),beta-(1-->6)-d-glucans during growth in hypoosmotic environments, and evidence is growing that these molecules may have a specific function during plant-microbe interactions in addition to osmoregulation. site-directed tn5 mutagenesis of the dna region upstream of ndvb resulted in identification of a new gene (ndvc) involved in beta-(1--> 3), beta-(1-->6)-glucan synthesis and in nodule development. the predicted translation pro ... | 1996 | 8755895 |
| desmutagenicity of soybean after heating. | the mutagenicity and desmutagenicity of extracts of soybeans heated at 225 +/- 5 degrees c were investigated by the ames test. the soybeans were refluxed in water, methanol, or diethylether for 2 h. the aqueous and methanol extracts (2-4 mg/plate) of the heated soybeans exhibited strong desmutagenic activity of 43-92% against heterocyclic amines (trp-p-1. glu-p-2, iq, meiqx. phip), while no mutagenicity was observed. the desmutagenicity of the heated soybean extracts remained even after denatura ... | 1996 | 8782410 |
| effects of processing method on degradation characteristics of protein and carbohydrate sources in situ. | two ruminally cannulated holstein cows in early lactation were used to determine the effects of heat treatment and particle size on fractional degradation rates and ruminal degradabilities of dm, cp and total nonstructural carbohydrate of dry shelled corn and soybeans. a randomized complete block design with cows as blocks was used. carbohydrate sources were cracked corn, chick cracked corn, finely ground corn, and steam-flaked corn. protein sources were soybeans that were roasted at 144 degrees ... | 1995 | 8786263 |
| control of ammonia formation during bacillus subtilis fermentation of legumes. | the control of ammonia formation during the bacillus subtilis fermentation of autoclaved, roasted soybean cotyledons (glycine max) and of autoclaved african locust bean cotyledons (parkia spp.) was investigated. addition of nacl, 1.5 mol (kg wet cotyledons)-1, part way through the fermentation inhibited ammonia formation and softening of the cotyledons. addition of glycerol, 1.7 mol (kg wet cotyledons)-1 part way through the fermentation inhibited alkalinisation and ammonia formation while allow ... | 1996 | 8796432 |
| aging of the human endometrium: peri-implantation phase endometrium does not show any age-dependent variation in lectin binding. | to evaluate if the peri-implantation endometrium shows age variations in lectin patterns, which suggest possible age variations in embryo-maternal recognition. | 1996 | 8801134 |
| alkaline-fermented foods: a review with emphasis on pidan fermentation. | alkaline-fermented foods constitute a group of less-known food products that are widely consumed in southeast asia and african countries. they can be made from different raw ingredients. for instance, japanese natto, thai thua-nao, and kinema are made from cooked soybeans, dawadawa from african locust beans, ogiri from melon seeds, ugba from african oil beans, kawal from fresh legale leaves, owoh from cotton seeds, and pidan from fresh poultry eggs. in alkaline-fermented foods, the protein of th ... | 1996 | 8817079 |
| genetic transformation, recovery, and characterization of fertile soybean transgenic for a synthetic bacillus thuringiensis cryiac gene. | somatic embryos of jack, a glycine max (l.) merrill cultivar, were transformed using microprojectile bombardment with a synthetic bacillus thuringiensis insecticidal crystal protein gene (bt cryiac) driven by the 35s promoter and linked to the hph gene. approximately 10 g of tissue was bombarded, and three transgenic lines were selected on hygromycin-containing media and converted into plants. the recovered lines contained the hph gene, but the bt gene was lost in one line. the plasmid was rearr ... | 1996 | 8819322 |
| a tnphoa insertion within the bradyrhizobium japonicum sips gene, homologous to prokaryotic signal peptidases, results in extensive changes in the expression of pbm-specific nodulins of infected soybean (glycine max) cells. | bradyrhizobium japonicum mutant 132 was obtained by random tnphoa mutagenesis of strain 110spc4. a 6.5 kb bamhi kanamycin-resistance-coding dna fragment of mutant 132 was used as a hybridization probe to clone the corresponding wild-type fragment. dna sequence analysis of a 3213 bp bamhi-clai fragment revealed that three open reading frames (orfs) were encoded in the same orientation. based on sequence similarities to other proteins in the database, the second orf was called sips (signal peptida ... | 1995 | 8825087 |
| cloning, expression and characterization of a blood group b active recombinant alpha-d-galactosidase from soybean (glycine max). | a cdna encoding soybean alpha-d-galactosidase [e.c. 3.2.1.22] was obtained by screening a soybean library with phaseolus alpha-d-galactosidase cdna. the glycine max alpha-d-galactosidase cdna is 1.75 kb long and contains untranslated 5' and 3' sequences. the deduced amino acid sequence of the soybean gene has a high degree of homology with other eucaryotic alpha-d-galactosidases. recombinant alpha-d-galactosidase (rgal) was expressed in pichia pastoris and purified by affinity chromatography. pu ... | 1996 | 8828798 |
| effect of feeding raw soybeans on polyamine metabolism in chicks and the therapeutic effect of exogenous putrescine. | the inclusion of isolated soy protein in milk replacer diets for calves and neonatal pigs inhibits development of intestinal mucosal cells. simultaneous administration of putrescine partially overcomes this effect. we therefore conducted experiments to determine the potential for dietary putrescine to overcome the toxicity of raw soybeans in chicks. in the first experiment, week-old chicks were fed either an isolated soy protein-based control diet or an isoenergetic and isonitrogenous diet conta ... | 1996 | 8856444 |
| characteristics of milk and reduced fat cheddar cheese from cows fed extruded soybeans and niacin. | a control diet and three experimental diets were fed to midlactation holstein cows to evaluate the effects of dietary unsaturated fatty acids and niacin on milk and reduced fat cheddar cheese. the diet consisted mainly of rolled corn and soybean meal in the concentrate mixture. experimental diets consisted of the control diet with added niacin and the control diet but with extruded soybeans substituted for part of the corn and soybean meal, with and without added niacin. milks from cows fed diet ... | 1996 | 8872710 |
| lactational responses of dairy cows fed unsaturated fat from extruded soybeans or sunflower seeds. | nine multiparous holstein cows were used in a replicated 3 x 3 latin square to evaluate the response to diets containing equal amounts of supplemental fat from extruded soybeans or sunflower seeds. both fat sources contained about 66% polyunsaturated fatty acids; for soybeans, about 8% was linolenic acid, but sunflower seeds contained mostly linoleic acid. periods were 5 wk, and data were collected the last 3 wk of each period. total mixed diets were (dm basis) 25% corn silage, 25% alfalfa hay, ... | 1996 | 8872718 |
| resistance gene analogs are conserved and clustered in soybean. | sequences of cloned resistance genes from a wide range of plant taxa reveal significant similarities in sequence homology and structural motifs. this is observed among genes conferring resistance to viral, bacterial, and fungal pathogens. in this study, oligonucleotide primers designed for conserved sequences from coding regions of disease resistance genes n (tobacco), rps2 (arabidopsis) and l6 (flax) were used to amplify related sequences from soybean [glycine max (l.) merr.]. sequencing of amp ... | 1996 | 8876208 |
| the effect of dietary fat inclusion on growth, carcass characteristics, and chemical composition of rabbits. | two hundred ten californian x new zealand white rabbits were used to study the influence of diet and slaughter weight (2.0, 2.25, and 2.5 kg) on feed intake, growth, feed efficiency, carcass characteristics, and body chemical composition. seven diets were formulated to contain 24% adf: diet c (control) had no added fat; diets t, o, and s contained 3% of added beef tallow, oleins, and soybean oil, respectively; diets tws, ows, and sws contained the same level and types of fat plus 18% heated whol ... | 1996 | 8880410 |
| effects of dietary fat with or without nicotinic acid on nutrient flow to the duodenum of dairy cows. | four holstein cows, fitted with ruminal and duodenal cannulas, were utilized in a 4 x 4 latin square design to investigate the effects of supplementing nicotinic acid to diets that contained 35% alfalfa haylage, 15% corn silage, and either 50% of a low fat concentrate or 10% whole raw soybeans and 40% of a high fat concentrate containing tallow. treatments in a 2 x 2 arrangement were 1) low fat, no supplemental nicotinic acid; 2) low fat, 12 g/d of nicotinic acid; 3) high fat, no supplemental ni ... | 1996 | 8880465 |
| molecular cloning of a human cdna encoding putative cysteine protease (prsc1) and its chromosome assignment to 14q32.1. | we have isolated a novel human cdna encoding a protein of 433 amino acids which shows 40% sequence identity to a hemoglobinase of schistosoma japonicum, one of the cysteine proteases in the pathway by which trematodes degrade host-cell globin. it also has 36% identity to a cysteine protease of the soybean glycine max that processes proproteins of various vacuolar proteins into mature form after translation. considering the sequence similarities among these proteins in such widely divergent speci ... | 1996 | 8893817 |
| roasted soybeans, blood meal, and tallow as sources of fat and ruminally undegradable protein in the diets of lactating cows. | the effect of roasted soybeans, blood meal, and tallow as sources of fat and rup for lactating dairy cows was studied. forty-five cows, blocked by age, calving date, and milk yield during the previous lactation, were assigned randomly to the following treatments (ingredient in the dm, rup as a percentage of cp, and fat in the dm, respectively): 1) soybean meal (16, 35, and 3.2%), 2) whole roasted soybeans (18, 40, and 6.2%), 3) ground roasted soybeans (18, 40, and 6.2%), 4) blood meal (2.7, 40, ... | 1996 | 8899527 |
| seasonal effects of supplemental fat or undegradable protein on the growth and metabolism of holstein calves. | one summer (heat stress) and one winter (neutral conditions) trial were conducted to assess the effects of varied amounts of rup and fat on the growth and metabolic responses of calves. in each trial, 190-kg holstein calves (n = 45) were fed a basal diet of corn and cottonseed hulls supplemented with soybean meal (control) or supplemented with raw or roasted whole soybeans or the basal ration plus prilled hydrogenated tallow either with or without a mixture of feather meal and blood meal. prille ... | 1996 | 8899528 |
| in vitro determination of ruminal protein degradation using freeze-stored ruminal microorganisms. | mixed ruminal microorganisms were harvested from a lactating dairy cow and preserved frozen or lyophilized. fermentation characteristics of freshly strained ruminal fluid, frozen microorganisms, or lyophilized microorganisms were evaluated during a 24-h pre-incubation and a 4-h incubation with test proteins. differences observed during the first 4 to 6 h in total amino acid concentration, optical density, ph and vfa concentrations, acetate:propionate ratio, and lactate concentration largely disa ... | 1996 | 8904719 |
| post-fertilization changes in the zona pellucida glycoproteins of rat eggs. | the zona pellucida (zp) is the extracellular coat surrounding the mammalian egg. numerous evidence supports the role of zp carbohydrate residues as the specific sperm receptors. in this study we used lectins to study different distribution patterns of carbohydrate residues in the rat zp, and to follow changes at fertilization. zp were collected from follicular, ovulated, and fertilized eggs, incubated with one of 11 different biotin-labeled lectins, followed by avidin-fluorescein isothiocyanate ... | 1996 | 8911967 |
| purification and molecular analysis of an extracellular gamma-glutamyl hydrolase present in young tissues of the soybean plant. | a polypeptide present in intercellular wash fluids of young leaves of glycine max has been purified to electrophoretic homogeneity. the protein has been identified as gamma-glutamyl hydrolase (ggh) based on the shared homology with a recently cloned cdna from rat. the enzyme is present within the extracellular space of young leaves and a portion is bound to the cell wall. northern and western analysis confirm that this polypeptide is expressed only in young (1-15 d old) leaf, stem and root tissu ... | 1996 | 8912628 |
| roasted soybeans and an estrogenic growth promoter affect growth hormone status and performance of beef steers. | this study investigated the effects of a roasted soybean (rsb)-supplemented diet and an estrogen implant (syn; synovex-s ear implant, 20 mg estradiol benzoate plus 200 mg progesterone) in young crossbred beef steers on their performance and plasma growth hormone (gh) response to challenge injections of thyrotropin-releasing hormone (trh) + gh-releasing hormone (ghrh). twenty individually fed steers (body weight 255 +/- 5 kg) were assigned to the following treatments: 1) no syn and fed a soybean ... | 1996 | 8914961 |
| soybeans versus animal sources of rumen-undergradable protein and fat for early lactation dairy cows. | fifteen multiparous holstein cows averaging 39 dim were used in a replicated 5 x 5 latin square design to compare roasted soybeans and animal by-products as supplements for increasing the rup content of lactation diets based on alfalfa. the control diet contained roasted soybeans and was formulated to meet requirements for rup. rumen-undegradable protein in the diet was increased by including additional roasted soybeans or animal by-product proteins. to achieve diets with similar amounts of fat, ... | 1996 | 8923252 |
| distribution of lectin binding sites in human bone marrow. identification by use of an ultrastructural postembedding technique. | the purpose of this ultrastructural study was to detect various carbohydrate residues on mature elements of the major human haematopoietic cell lines (granulopoiesis, erythropoiesis and megakaryopoiesis), sinus endothelium and plasma cells under comparable experimental conditions. marrow specimens were processed according to a modified postembedding technique with unicryl as embedding resin. a broad panel of 10 digoxigenin (dig)-conjugated lectins was applied for staining and specificity was eva ... | 1996 | 8933737 |
| characterization of soybean choline kinase cdnas and their expression in yeast and escherichia coli. | an expressed sequence tag from arabidopsis that displayed sequence homology to mammalian and yeast choline kinases was used to isolate choline kinase-like cdnas from soybean (glycine max l.). two distinct cdnas, designated gmck1 and gmck2, were recovered that possessed full-length reading frames, each sharing approximately 32% identity at the predicted amino acid level with the rat choline kinase. a third unique choline kinase-like cdna, gmck3, was also identified but was not full length. hetero ... | 1996 | 8934624 |
| cloning of an enzyme that synthesizes a key nucleotide-sugar precursor of hemicellulose biosynthesis from soybean: udp-glucose dehydrogenase. | hemicellulose is a major component of primary plant cell walls. many of the glycosyl residues found in hemicellulose are derived from the sugar precursor udp-glucuronic acid, which can be converted into udp-arabinose, udp-apiose, udp-galacturonic acid, and udp-xylose. the enzyme controlling the biosynthesis of udp-glucuronic acid, udp-glucose dehydrogenase (ec 1.1.1.22), was cloned from soybean (glycine max [l.] merr.) by an antibody screening procedure. this enzyme is surprisingly homologous to ... | 1996 | 8938413 |
| differential expression of soybean cysteine proteinase inhibitor genes during development and in response to wounding and methyl jasmonate. | three cysteine proteinase inhibitor cdna clones (pl1, pr1, and pn2) have been isolated from a soybean (glycine max l. merr.) embryo library. the proteins encoded by the clones are between 60 and 70% identical and contain the consensus qxvxg motif and w residue in the appropriate spatial context for interaction with the cysteine proteinase papain. l1, r1, and n2 mrnas were differentially expressed in different organs of plants (juvenile and mature) and seedlings, although n2 mrna was constitutive ... | 1996 | 8938418 |
| an extract of soy flour influences serum cholesterol and thyroid hormones in rats and hamsters. | the effects of an ethanol-acetone extract from soy flour on serum lipids and thyroid hormones were studied in rats and hamsters. in study 1, male sprague-dawley rats were fed diets containing protein from ethanol-acetone extracted isolated soy protein (isp-), nonextracted isp (isp), casein (casein-), or casein to which 0.36 mg ethanol-acetone extract/g protein was added (casein+). rats fed either isp-containing diet had lower serum total cholesterol concentrations compared with those fed either ... | 1996 | 9001373 |
| roasted soybeans and an estrogenic growth promoter affect the thyroid status of beef steers. | we investigated the interactive effects of a roasted soybean (rsb)-supplemented diet and an estrogen ear implant [synovex-s (syn), 20 mg estradiol benzoate + 200 mg progesterone] in young beef steers on measures of thyroid status before and after challenge injections of thyrotropin-releasing hormone (trh) + growth hormone-releasing hormone (ghrh). twenty steers (body weight 255 +/- 5 kg) were assigned to the following treatments: 1) no syn and a soybean meal-supplemented diet, 2) no syn and a rs ... | 1997 | 9039839 |
| performance and profiles of milk fatty acids of cows fed full fat, heat-treated soybeans using various processing methods. | twenty-four multiparous holstein cows (48 d in lactation) were used in an 8-wk trial employing a completely randomized block design to determine the effect of heat treatment of full fat soybeans on the fatty acid composition of milk fat. treatments consisted of a total mixed ration supplemented with 1) ground raw, 2) extruded, 3) micronized, or 4) roasted soybeans included at 17.5% of the dry matter of the total mixed ration. dietary treatment had no effect on body weight, yields of milk or soli ... | 1997 | 9058276 |
| a novel radioimmunoassay for daidzein. | a radioimmunoassay for daidzein was established, based on polyclonal antibodies against daidzein-4'-o-(carboxymethyl)ether-bsa. the sensitivity of the assay was 0.4 pg/tube; the intra- and interassay coefficients of variation varied from 4.1 to 11.5% and from 5.6 to 21.7%, respectively, depending upon the method (direct or extraction) and concentration of daidzein in the sample. the cross reactivities with other chemically related compounds, with the exception of 4'-derivatives of daidzein, were ... | 1997 | 9071741 |
| nutritional value for swine of soybean meal produced by isopropyl alcohol extraction. | three experiments were conducted to compare the nutritional value of soybean meal produced by extraction with 95% isopropyl alcohol (ipa) or hexane (hex) for swine. the same batch of soybeans and the same processing equipment were used to produce both soybean meals. analyzed contents of the ipa and hex meals were, respectively: cp, 48.7, 47.0%; lysine, 3.11, 3.06%; urease, .24, .13 delta ph. in exp. 1, two soybean meals and dietary lysine at .90 or 1.25% were used in a 2 x 2 factorial arrangemen ... | 1997 | 9078488 |
| pathogenesis and treatment of liver fibrosis in alcoholics: 1996 update. | fibrosis is a common end stage for most chronic liver diseases. it results from an imbalance between collagen production and degradation. one promising approach for prevention and treatment is the stimulation of collagenolytic processes. in nonhuman primates it was found that polyenylphosphatidylcholine (ppc), extracted from soybeans, protects against alcohol-induced fibrosis and cirrhosis and prevents the associated hepatic phosphatidylcholine (pc) depletion by increasing 18:2-containing pc spe ... | 1997 | 9101129 |
| soybean dapa mutations encoding lysine-insensitive dihydrodipicolinate synthase. | in plants, the rate-limiting step in the pathway for lysine synthesis is catalyzed by the enzyme dihydrodipicolinate synthase (ds), which is encoded by the dapa gene. we previously cloned the soybean (glycine max cv. century) dapa gene in escherichia coli to express functional soybean ds protein. like the wild-type soybean ds enzyme, the ds activity encoded by the cloned gene was extremely sensitive to feedback inhibition by micromolar concentrations of lysine. three mutants of the soybean dapa ... | 1997 | 9106516 |
| map order and linkage distances of molecular markers close to the supernodulation (nts-1) locus of soybean. | the molecular characteristics of markers in the chromosome region surrounding the supernodulation gene (nts-1) of soybean (glycine max l. merr.) were investigated in 187 f2 plants from a cross of g. max cv. bragg (nts) and g. soja pi468.397 (wild-type nodulation). rflp marker putg-132a, linked tightly (0.7+/-0.5 cm) to nts-1, was converted to a pcr marker. the polymorphism resides within a 1.72 kb psti fragment and consists of an 832 bp insertion in g. max relative to the wild progenitor g. soja ... | 1997 | 9108287 |
| novel, highly expressed late nodulin gene (ljnod16) from lotus japonicus. | we have isolated a lotus japonicus cdna corresponding to a highly abundant, late nodule-specific rna species that encodes a polypeptide with a predicted molecular mass of 15.6 kd. the protein and its corresponding gene were designated nlj16 and ljnod16, respectively. ljnod16 was found to be expressed only in the infected cells of l. japonicus nodules. related dna sequences could be identified in the genomes of both glycine max and medicago sativa. in the latter, a homologous mrna species was det ... | 1997 | 9112769 |
| a blend of animal and cereal protein or fish meal as partial replacement for soybean meal in the diets of lactating holstein cows. | six replications in experiment 1 and four replications in experiment 2 of a 3 x 3 latin square arrangement of treatments were used to compare soybean meal or soybean meal partially replaced with fish meal or a protein blend for response in intake, milk yield and composition, ruminal nh3 n, blood urea, and ruminal fermentation in lactating holstein cows. the blend contained 30% corn gluten meal, 30% poultry by-products, 30% blood meal, and 10% feather meal. periods were 28 d, and the first 7 d we ... | 1997 | 9120086 |
| polymerase chain reaction (pcr) in the quality and safety assurance of food: detection of soya in processed meat products. | a new method for the specific and sensitive detection of soya (glycine max) in processed meat products has been developed using polymerase chain reaction (pcr) technology. the presence of soya deoxyribonucleic acid (dna) from several soya protein concentrates was determined with two pairs of specific oligonucleotides yielding a 414-bp (bp = base pair) fragment and an internal 118-bp fragment amplified from the soya lectin le1 gene. the test detected dna from textured soya protein concentrates in ... | 1996 | 9123971 |
| kinetic mechanism and cofactor content of soybean root nodule urate oxidase. | the kinetic mechanism of urate oxidase isolated from soybean root nodules has been determined by initial velocity kinetic studies monitoring oxygen uptake, in order to avoid potential artifacts in the spectrophotometric assay which arise from absorbance due to unidentified products of the enzymatic reaction. urate and o2 bind to the enzyme sequentially; xanthine is a competitive inhibitor versus urate and a noncompetitive inhibitor versus o2, which suggests that urate binds to the enzyme before ... | 1997 | 9125493 |
| expression of glycine max (soybean agglutinin) binding molecule in injured motoneurons and its specific localization in the extracellular matrix between injured neurons and microglia. | in order to identify expression of molecules that are secreted or translocated into extracellular regions after nerve injury, lectin histochemistry using 16 lectins was carried out. after unilateral hypoglossal nerve transection, only glycin max (soybean agglutinin; sba) staining was apparent in the injured hypoglossal nucleus. the sba staining was observed clearly from 3 days after the operation, and the most intense staining was observed around postoperative day 5-7. thereafter sba staining gr ... | 1997 | 9129185 |
| [immunologic analysis of milk, hen egg, and soybean proteins in butter and margarine, and clinical assessment for availability of hypoallergenic margarine (ham)]. | to determine the allergenic proteins in commercially available butter and margarine, protein fractions were extracted and immunologically analyzed against milk, hen egg, and soybean antigens. butter and 10 kinds of margarine were proved to contain these proteins in various concentrations by elisa and immunoblotting methods by use of the rabbit antisera developed against each food proteins. however, hypoallergenic margarine was found to contain no such proteins at all. using sera obtained from at ... | 1996 | 9133334 |
| interaction of proteases with legume seed inhibitors. molecular features. | after having found that raw black beans (phaseolus vulgaris) were toxic, while the cooked ones constitute the basic diet of the underdeveloped peoples of the world, in the sixties, our research directed by dr. jaffé, concentrated mainly around the detection and identification of the heat labile toxic factors in legume seeds. a micromethod for the detection of protease inhibitors (pi) in individual seeds was developed, for the purpose of establishing that the multiple trypsin inhibitors (ti) foun ... | 1996 | 9137634 |
| protein proteinase inhibitors in legume seeds--overview. | protein proteinase inhibitors are widely distributed in plant seeds, particularly in legumes. the specificity and potency of inhibition depend on defined inhibitory sites and on the animal species of the target proteinase. feeding experiments on diets containing isolated soybean trypsin inhibitors (the kunitz soybean trypsin inhibitor sti and the bowman-birk trypsinchymotrypsin inhibitor bbi) caused insignificant growth depression in rats and chicks, but induced enlargement of the pancreas in ra ... | 1996 | 9137635 |
| eating to beat breast cancer: potential role for soy supplements. | the study reviews the anticancer properties of natural isoflavones which occur in especially high concentration in soybeans. it considers the suitability of soybean products for clinical trials aiming to reduce the progression of breast cancer. | 1997 | 9137789 |
| inhibitory activity of soyasaponin ii on virus replication in vitro. | the antiviral activities of two saponins, soyasaponin i and ii, isolated from soybean (glycine max merrill) were studied in vitro against herpes simplex virus type 1 (hsv-1). soyasaponin ii was more potent than soyasaponin i as shown by reduction of hsv-1 production. soyasaponin ii was also found to inhibit the replication of human cytomegalovirus, influenza virus, and human immunodeficiency virus type 1. the action was not due to inhibition of virus penetration and protein synthesis, but might ... | 1997 | 9140220 |
| purification and characterization of a soybean hull allergen responsible for the barcelona asthma outbreaks. ii. purification and sequencing of the gly m 2 allergen. | a low mw allergen from soybean hull, gly m 1, with two isoallergens, gly m 1 a and gly m 1 b, was associated with the asthma outbreaks that occurred in cartagena, spain. using sera of asthmatic epidemic patients (aep) from barcelona, three main soybean hull allergens, two of them with mws and pis identical to those reported for gly m 1 a and gly m 1 b, were identified. | 1997 | 9146936 |
| [development of an intermediate moisture product for the elderly based on texturized soybeans]. | in accordance with the fact the aging process is closely related to the individual nutrition, it is possible to infer that the daily diet could help and improve, to a certain degree, to the elderly well-being. based on this and making use of the food technology expertise, the development of a low cost, easily prepared and preserved product, in accordance with the taste and needs of the elderly people was considered. the objective of this project was to elaborate an intermediate moisture food (im ... | 1995 | 9161450 |
| curcumin and genistein, plant natural products, show synergistic inhibitory effects on the growth of human breast cancer mcf-7 cells induced by estrogenic pesticides. | curcumin and genistein are two natural products of plants obtained from curcuma longa linn (turmeric) and soybeans, respectively. both compounds when present at micromolar concentrations are able to inhibit the growth of estrogen-positive human breast mcf-7 cells induced individually or by a mixture of the pesticides endosulfane, ddt and chlordane or 17-beta estradiol. when curcumin and genistein were added together to mcf-7 cells, a synergistic effect resulting in a total inhibition of the indu ... | 1997 | 9168916 |
| digestible sulfur amino acid requirement of starting turkeys. | three experiments (a total of 1,020 poults) were conducted to determine the digestible sulfur amino acid (saa) requirement for female turkey poults during the starter period. poults were fed a standard corn-soybean meal diet (pc) that met or exceeded nrc recommendations (28% cp, 3,172 kcal men/kg) for 1 wk and were then randomly assigned to treatments until 22 d (experiment 2 and 3) or 23 d (experiment 1) of age. dietary treatments included the pc diet and seven or nine titrated levels of methio ... | 1997 | 9181621 |
| soybean (glycine max) agglutinin binds to corneal endothelial cells during wound repair and alters their microfilament pattern. | in the present study we have examined soybean (glycine max) agglutinin (sba) binding to cells of the rat corneal endothelium during wound repair. circular transcorneal freeze injuries were given to the endothelia and the tissues were organ cultured at 37 degrees c in basal media eagle with 10% serum for up to 72 hrs. sba failed to bind to the surface of non-injured corneal endothelium, but strongly bound to cells involved in the wound repair process. punctate surface binding was detected 24 hrs. ... | 1997 | 9193787 |
| response of lactating cows to methionine or methionine plus lysine added to high protein diets based on alfalfa and heated soybeans. | lactation diets based on wilted alfalfa silage and heated whole soybeans are common in the midwestern us. we examined the milk production response of multiparous holstein cows to the addition of ruminally protected methionine at two percentages to a basal total mixed ration. an additional total mixed ration included both methionine and lysine supplementation. sixteen holstein cows in early lactation were used in a replicated 4 x 4 latin square design with 21-d periods. milk production, milk comp ... | 1997 | 9201591 |
| amino acid composition, available lysine content and in vitro protein digestibility of selected tropical crop seeds. | as the search for alternative sources of food to alleviate hunger continues, this study was undertaken to determine nitrogen and amino acid content, chemical score, protein digestibility corrected amino acid score, available lysine and in vitro digestibility of 8 lesser known, wild tropical seeds, gathered in nigeria. results were contrasted with a tropical soybean variety (glycine max, tgx 1660-15f). the investigated seeds were millettia thonningii, gliricidia sepium, lonchocarpus sericeus, alb ... | 1997 | 9201749 |
| multi-year monitoring of canadian grains and grain-based foods for trichothecenes and zearalenone. | monitoring of canadian grain crops and foods by the health protection branch for deoxynivalenol (don, vomitoxin) has been undertaken every year since 1980, when it was found in ontario soft wheat for the first time (in the 1979 and 1980 crops). contamination of this wheat crop has varied, with 22-100% incidences in all but 1 year and up to 0.75 micrograms/g for the annual means of positive samples. the canadian guideline for don is 2 micrograms/g in uncleaned soft wheat. western canadian hard wh ... | 1997 | 9205561 |