| identification and expression of the kosena radish (raphanus sativus cv. kosena) homologue of the ogura radish cms-associated gene, orf138. | a cms-associated gene, orf125, present in the japanese radish cultivar kosena, has a sequence homologous to that of the ogura cms-associated gene, orf138, except for two amino acid substitutions and a 39 bp deletion in the orf138 coding region. in kosena radish, orf125 is linked with orfb, whereas the orf125 locus differs in a brassica napus cms cybrid derived from protoplast fusion between kosena radish and b. napus. a novel mtdna sequence is present in the 3'-flanking region of orf125 in the b ... | 1999 | 10080721 |
| n-3 enrichment of chicken meat using fish oil: alternative substitution with rapeseed and linseed oils. | two sequential experiments were conducted to assess the effect of replacing a fish oil diet with vegetable oil diets on broiler chicken performance and fatty acid (fa) composition and sensory traits of broiler meat. a diet enriched with 8.2% fish oil (fo) was fed to the birds throughout the 5-wk growth period (t1), the same basal diet being supplemented with 8.2% linseed oil (lo, experiment 1) or rapeseed oil (ro, experiment 2) in three different periods: the last week before slaughtering at 35 ... | 1999 | 10090262 |
| lymphatic fatty acid absorption profile during 24 hours after administration of triglycerides to rats. | in this study we determined in rats the complete 24-h lymphatic fatty acid profile after administration of either rapeseed oil (ro) or rapeseed oil interesterified with 10:0 (ro/c10) with special emphasis on the transition from absorptive to postabsorptive phase. rats were subjected to cannulation of the main mesenteric lymph duct and the next day oils were administered through a gastric feeding tube. lymph was collected in 1-h fractions for the following 24 h. the time for maximum lymphatic tra ... | 1999 | 10102235 |
| characterization of low-molecular-mass trypsin isoinhibitors from oil-rape (brassica napus var. oleifera) seed. | a new low-molecular-mass (6767.8 da) serine proteinase isoinhibitor has been isolated from oil-rape (brassica napus var. oleifera) seed, designated 5-oxopro1-gly62-rti-iii. the 5-oxopro1-gly62-rti-iii isoinhibitor is longer than the asp2-pro61-rti-iii and the ser3-pro61-rti-iii forms, all the other amino acid residues being identical. in rti-iii isoinhibitors, the p1-p1' reactive site bond (where residues forming the reactive site have been identified as pnellipsisp1 and p1'ellipsispn', where p1 ... | 1999 | 10103060 |
| identification of delta12-fatty acid desaturase from arachidonic acid-producing mortierella fungus by heterologous expression in the yeast saccharomyces cerevisiae and the fungus aspergillus oryzae. | based on the sequence information for the omega3-desaturase genes (from brassica napus and caenorhabditis elegans), which are involved in the desaturation of linoleic acid (delta9, delta12-18 : 2) to alpha-linolenic acid (delta9, delta12, delta15-18 : 3), a cdna was cloned from the filamentous fungal strain, mortierella alpina 1s-4, which is used industrially to produce arachidonic acid. homology analysis with protein databases revealed that the amino acid sequence showed 43.7% identity as the h ... | 1999 | 10215899 |
| influence of dietary rapeseed oil, vitamin e, and copper on the performance and the antioxidative and oxidative status of pigs. | we investigated the effects of dietary copper and vitamin e in diets containing 6% rapeseed oil on the performance and the antioxidative and oxidative status of growing pigs. the 10 dietary treatments consisted of a basal diet (9 mg of vitamin e/kg feed, 15 mg of cu/kg feed), the basal diet + 6% rapeseed oil (diet 1; 18 mg of vitamin e/kg feed, 15 mg of cu/kg feed), and diet 1 plus supplements of vitamin e (0, 100, and 200 mg of dl-alpha-tocopheryl acetate/kg feed) and copper (0, 35, and 175 mg ... | 1999 | 10328356 |
| developmental toxicity study of vegetable oil-derived stanol fatty acid esters. | in a standard developmental toxicity study, a mixture of vegetable oil-derived stanol fatty acid esters was administered in the diet to groups of 28 mated female hsdcpb:wu wistar rats at concentrations that provided 0, 1, 2.5, and 5% total stanols (equivalent to 0, 1.75, 4.38, and 8.76% plant stanol esters). test diets were adjusted with rapeseed oil to maintain an equivalent caloric content of fatty acids at each of the treatment levels. the treatment period extended from day 0 to 21 of gestati ... | 1999 | 10341155 |
| inter-organ signaling in plants: regulation of atp sulfurylase and sulfate transporter genes expression in roots mediated by phloem-translocated compound. | sulfate uptake and atp sulfurylase activity in the roots of arabidopsis thaliana and brassica napus were enhanced by s deprivation and reduced following resupply of so4(2-). similar responses occurred in split-root experiments where only a portion of the root system was s-deprived, suggesting that the regulation involves inter-organ signaling. phloem-translocated glutathione (gsh) was identified as the likely transducing molecule responsible for regulating so4(2-) uptake rate and atp sulfurylase ... | 1999 | 10341446 |
| effects of dietary polyunsaturated fatty acids on rat brain plasma membrane fatty acid composition. | the effects of four different diets on phospholipid fatty acid composition of rat brain plasma membranes were evaluated. rats were given a semisynthetic diet in which lipids were supplied by 5% peanut oil (n-3 pufa deficient diet), cod liver oil (n-6 pufa deficient diet), partially hydrogenated palm oil (total pufa deficient diet) or a mixture of peanut and rapeseed oil (control group). animals fed the total pufa deficient diet had significantly lower body and brain weights than the control grou ... | 1998 | 10347691 |
| an example of intron junctional sliding in the gene families encoding squalene monooxygenase homologues in arabidopsis thaliana and brassica napus. | sequences of three arabidopsis thaliana and two brassica napus cdnas encoding squalene monooxygenase homologues (sqp1 and sqp2) are reported. southern analysis confirmed that these cdnas are derived from small gene families in both species. expression analysis indicates that sqp1 genes in b. napus are strongly expressed in leaves but not roots or developing seeds. comparison of cdna and genomic sequences indicate that the 3' splice site of an intron in these genes has undergone junctional slidin ... | 1999 | 10350086 |
| genetically engineered plant allergens with reduced anaphylactic activity. | allergy immunotherapy is based on the administration of increasing amounts of the disease-eliciting allergens in order to yield allergen-specific non-responsiveness. success of this therapy is associated with modulation of the immune response to allergenic molecules at the level of t-helper cells and the induction of blocking antibodies. the extracts used for immunotherapy are highly heterogenous preparations from natural sources and contain additional components, mostly proteins which are not w ... | 1999 | 10394098 |
| lysophosphatidic acid acyltransferase from coconut endosperm mediates the insertion of laurate at the sn-2 position of triacylglycerols in lauric rapeseed oil and can increase total laurate levels | expression of a california bay laurel (umbellularia californica) 12:0-acyl-carrier protein thioesterase, bay thioesterase (bte), in developing seeds of oilseed rape (brassica napus) led to the production of oils containing up to 50% laurate. in these bte oils, laurate is found almost exclusively at the sn-1 and sn-3 positions of the triacylglycerols (t.a. voelker, t.r. hayes, a.c. cranmer, h.m. davies [1996] plant j 9: 229-241). coexpression of a coconut (cocos nucifera) 12:0-coenzyme a-preferri ... | 1999 | 10398708 |
| characterization and immunolocalization of a cytosolic calcium-binding protein from brassica napus and arabidopsis pollen. | two low-molecular-weight proteins have been purified from brassica napus pollen and a gene corresponding to one of them has been isolated. the gene encodes an 8.6-kd protein with two ef-hand calcium-binding motifs and is a member of a small gene family in b. napus. the protein is part of a family of pollen allergens recently identified in several evolutionarily distant dicot and monocot plants. homologs have been detected in arabidopsis, from which one gene has been cloned in this study, and in ... | 1999 | 10398714 |
| a plastidial lysophosphatidic acid acyltransferase from oilseed rape. | the biosynthesis of phosphatidic acid, a key intermediate in the biosynthesis of lipids, is controlled by lysophosphatidic acid (lpa, or 1-acyl-glycerol-3-p) acyltransferase (lpaat, ec 2.3.1.51). we have isolated a cdna encoding a novel lpaat by functional complementation of the escherichia coli mutant plsc with an immature embryo cdna library of oilseed rape (brassica napus). transformation of the acyltransferase-deficient e. coli strain jc201 with the cdna sequence bat2 alleviated the temperat ... | 1999 | 10398728 |
| fatty acid anilides: in vivo formation and relevance to toxic oil syndrome. | toxic oil syndrome (tos), a multisystemic epidemic outbreak in 1981 in spain, was caused by the ingestion of a cooking oil mixture containing rapeseed oil denatured with aniline. the mechanisms and causative agents responsible for the tos are still not known. although primary lesions observed in tos patients could not be reproduced experimentally, the levels of fatty acid anilides (faas) and aniline in tos-related cooking oil were considered proximate markers of tos. aniline, available from free ... | 1999 | 10402561 |
| absorption in rats of rapeseed, soybean, and sunflower oils before and following moderate heating. | rapeseed, soybean, and sunflower oil were heated for 15 min in a 5-mm oil layer in a pan at 180 degrees c. the fatty acid composition was almost unaffected by heating, while the polymer content rose slightly and the tocopherol content decreased, except in soybean oil. the absorption of oils before and after heating was investigated in lymph-cannulated rats. oils were administered as emulsions through a gastrostomy tube and lymph was collected during the next 24 h. the highest accumulated lymphat ... | 1999 | 10478931 |
| long-term n-3 fatty acid deficiency induces no substantial change in the rate of protein synthesis in rat brain and liver. | the influence of long-term n-3 fatty acid deficiency on the rate of protein synthesis in rat brain and liver was investigated in relation to learning behavior or a presumed survival time-shortening factor (ssf) in rapeseed oil, using a large-dose [3h]phenylalanine (phe) injection method. when wistar rats were made n-3 fatty acid-deficient by feeding a safflower oil (alpha-linolenate-deficient) diet for 2 generations, conditions under which the safflower oil group had been shown to exhibit altere ... | 1999 | 10480312 |
| factor analysis of digestive cancer mortality and food consumption in 65 chinese counties. | dietary factors were analyzed for the regional difference of gi tract cancer mortality rates in china. sixty-five rural counties were selected among a total of 2,392 counties to represent a range of rates for seven most prevalent cancers. the dietary data in the selected 65 counties were obtained by three-day dietary record of households in 1983. the four digestive cancer mortality rates (annual cases per 100,000 standardized truncated rates for ages 35-64) and per capita food consumption were a ... | 1999 | 10510586 |
| the effect of rapeseed oil uptake on the production of erythromycin and triketide lactone by saccharopolyspora erythraea. | saccharopolyspora erythraea was grown in an oil-based process medium at two different laboratory scales. the initial concentration of rapeseed oil in the medium was shown not to affect the growth, while addition of oil significantly increased erythromycin a production. increasing the agitation speed at the 2 l scale increased the growth of the culture and the production of erythromycin a but had little effect on the level of oil remaining in the fermentation. maximum oil utilization of 50% (w/w) ... | 1999 | 10514261 |
| inhibitor binding studies on enoyl reductase reveal conformational changes related to substrate recognition. | enoyl acyl carrier protein reductase (enr) is involved in fatty acid biosynthesis. in escherichia coli this enzyme is the target for the experimental family of antibacterial agents, the diazaborines, and for triclosan, a broad spectrum antimicrobial agent. biochemical studies have suggested that the mechanism of diazaborine inhibition is dependent on nad(+) and not nadh, and resistance of brassica napus enr to diazaborines is thought to be due to the replacement of a glycine in the active site o ... | 1999 | 10521472 |
| dietary docosahexaenoic acid-enriched phospholipids normalize urinary melatonin excretion in adult (n-3) polyunsaturated fatty acid-deficient rats. | melatonin (mel) plays an essential role in physiologic functions associated with darkness. we examined the effects of docosahexaenoic acid (dha)-enriched phospholipids from pig brains (bpl) or hen eggs (epl), as sources of dha, on lipid fa composition of pineal membranes and daytime and nighttime concentrations of 6-sulfatoxymelatonin (amt6) in adult male control and (n-3)-deficient rats fed bpl and epl diets for 5 wk. in two experiments, at 3 wk of age, rats were divided into subgroups and fed ... | 1999 | 10539787 |
| replacing saturated fat with pufa-rich (sunflower oil) or mufa-rich (rapeseed, olive and high-oleic sunflower oil) fats resulted in comparable hypocholesterolemic effects in cholesterol-fed hamsters. | recent studies have suggested that monounsaturated fatty acid (mufa)-rich dietary fats do not have the same plasma cholesterol-lowering effects whereby rapeseed oil was more effective than olive oil. this phenomenon could be explicable by the content of other fatty acids or plant sterols. to further evaluate the effects of different mufa-rich oils (18:1-rich sunflower oil, rapeseed oil, olive oil) in comparison to polyunsaturated (pufa)-rich oils (18:2-rich sunflower oil) and saturated fat (palm ... | 1999 | 10545672 |
| green fluorescent protein as a marker for expression of a second gene in transgenic plants. | the use of transgenic crops has generated concerns about transgene movement to unintended hosts and the associated ecological consequences. moreover, the in-field monitoring of transgene expression is of practical concern (e.g., the underexpression of an herbicide tolerance gene in crop plants that are due to be sprayed with herbicide). a solution to these potential problems is to monitor the presence and expression of an agronomically important gene by linking it to a marker gene, such as gfp. ... | 1999 | 10545923 |
| poly(beta-hydroxybutyrate) production in oilseed leukoplasts of brassica napus | polyhydroxyalkanoates (phas) comprise a class of biodegradable polymers which offer an environmentally sustainable alternative to petroleum-based plastics. production of phas in plants is attractive since current fermentation technology is prohibitively expensive. the pha homopolymer poly(beta-hydroxybutyrate) (phb) has previously been produced in leaves of arabidopsis thaliana (nawrath et al., 1994, proc natl acad sci usa 91: 12760-12764). however, brassica napus oilseed may provide a better sy ... | 1999 | 10550638 |
| influence of dietary fat and vitamin e on antioxidant status of muscles of turkey. | the aim of this study was to better understand the effects of more or less unsaturated fat source (tallow/soy oil/rapeseed oil) and/or vitamin e dietary supplementation (200 ppm) on the antioxidant status (at day 1 post-mortem) of turkey muscles [pectoralis major (pm) and sartorius (s)]. more particularly, when turkeys were fed tallow, supplementation was sufficient to improve significantly the vitamin e status. feeding rapeseed oil increased the antioxidant enzyme (aoe) activities (catalase, su ... | 1999 | 10563878 |
| age-dependent wound induction of a myrosinase-associated protein from oilseed rape (brassica napus). | in order to study the expression of the induced form of myrosinase-associated protein (imyap), a genomic clone encoding the protein was isolated from brassica napus. the coding portion of the gene was found to consist of five exons separated by one long intron of 938 bp and three shorter introns of ca. 100 bp. a 1.9 kb promoter fragment including the 5'-untranslated region was cloned in front of the coding portion of the escherichia coli iuda gene and transformed into arabidopsis thaliana. expre ... | 1999 | 10579485 |
| [insect-resistant transgenic plants of brassica napus and analysis of resistance in the plants]. | cotyledons, each with a 1-2 mm petiole at its base, were cut from axenic seedlings and infected with agrobacterium tumefaciens. after 2-3 days of cocultivation, the cotyledon explants were transferred to ms selection medium containing 15 mg/l kanamycin and 4.5 mg/l 6-ba to induce shoot differentiation. kanamycin-resistant shoots were subcultured on selection medium with 20-50 mg/l kanamycin for 3-6 months for eliminating escaped non-transformants, and then rooted on ms medium containing 25 mg/l ... | 1999 | 10589167 |
| genes expressed in pinus radiata male cones include homologs to anther-specific and pathogenesis response genes. | we describe the isolation and characterization of 13 cdna clones that are differentially expressed in male cones of pinus radiata (d. don). the transcripts of the 13 genes are expressed at different times between meiosis and microspore mitosis, timing that corresponds to a burst in tapetal activity in the developing anthers. in situ hybridization showed that four of the genes are expressed in the tapetum, while a fifth is expressed in tetrads during a brief developmental window. six of the seven ... | 1999 | 10594098 |
| functional expression and characterization of the myrosinase myr1 from brassica napus in saccharomyces cerevisiae. | myrosinases are thioglucosidases that hydrolyze the natural plant products glucosinolates. we have expressed the myrosinase myr1 from brassica napus in saccharomyces cerevisiae. the recombinant myrosinase was enzymatically active which shows that the myr1, which in the plant is complex bound with myrosinase-binding proteins and myrosinase-associated proteins, is functional in its free form. characterization of the recombinant myr1 with respect to ph optimum, substrate specificity, activation by ... | 1999 | 10600460 |
| dietary docosahexaenoic acid ameliorates, but rapeseed oil and safflower oil accelerate renal injury in stroke-prone spontaneously hypertensive rats as compared with soybean oil, which is associated with expression for renal transforming growth factor-beta, fibronectin and renin. | we have noted that n-3 fatty acid-rich oils, such as fish oil, perilla oil and flaxseed oil as well as ethyl docosahexaenoate (dha) prolonged the survival time of stroke-prone spontaneously hypertensive rats (shrsp) rats by approximately 10% as compared with linoleate (n-6)-rich safflower oil. rapeseed oil with a relatively low n-6/n-3 ratio unusually shortened the survival time by approximately 40%, suggesting the presence of minor components unfavorable to shrsp rats. this study examined the e ... | 2000 | 10601699 |
| crystallographic analysis of triclosan bound to enoyl reductase. | molecular genetic studies with strains of escherichia coli resistant to triclosan, an ingredient of many anti-bacterial household goods, have suggested that this compound works by acting as an inhibitor of enoyl reductase (enr) and thereby blocking lipid biosynthesis. we present structural analyses correlated with inhibition data, on the complexes of e. coli and brassica napus enr with triclosan and nad(+) which reveal how triclosan acts as a site-directed, picomolar inhibitor of the enzyme by m ... | 1999 | 10610777 |
| polyunsaturated fatty acids in the food chain in europe. | intakes of partially hydrogenated fish oil and animal fats have declined and those of palm, soybean, sunflower, and rapeseed oils have increased in northern europe in the past 30 y. soybean and rapeseed oils are currently the most plentiful liquid vegetable oils and both have desirable ratios of n-6 to n-3 fatty acids. however, soybean and rapeseed oils are commonly partially hydrogenated for use in commercial frying to decrease susceptibility to oxidative degradation. this process leads to sele ... | 2000 | 10617968 |
| crystallization of the nadp-dependent beta-keto acyl-carrier protein reductase from brassica napus. | the nadp-dependent beta-keto acyl-carrier protein reductase (bkr) from brassica napus has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol of average molecular weight 1500 as the precipitant. the crystals belong to the hexagonal space group p6(4)22, with unit-cell parameters a = b = 129. 9, c = 93.1 a, alpha = beta = 90, gamma = 120 degrees. calculated values for v(m), the use of rotation and translation functions and consideration of the packing suggest th ... | 2000 | 10666637 |
| incorporation of different polyunsaturated fatty acids into eggs. | an experiment was carried out to examine thoroughly the relationships among different n-3 and n-6 polyunsaturated fatty acids (pufa) in the diet, their deposition into the eggs' fat, and their effect on hens' laying performance. a diet enriched with 4% fish oil (fo) was fed to the birds throughout the 14-wk laying period (treatment 1; t1); this was the same oil source that was replaced in proportions of 25, 50, 75, or 100% with four different fat sources, resulting in 17 isocaloric dietary treat ... | 2000 | 10685889 |
| the effect of dietary trans alpha-linolenic acid on plasma lipids and platelet fatty acid composition: the transline study. | to collect (i) baseline data and (ii) execute a large multicentre study examining the effect of trans alpha-linolenic acid on its incorporation into plasma lipids and on risk factors for coronary heart disease. | 2000 | 10694780 |
| a binary vector-based large insert library for brassica napus and identification of clones linked to a fertility restorer locus for ogura cytoplasmic male sterility (cms). | we constructed and characterized a large dna insert library for brassica napus that would facilitate genome-related research and map-based cloning efforts in brassica species. this library, consisting of 92,160 clones arrayed in 384-well microtiter dishes, was based on a conventional plant transformation vector (binary vector), and was constructed using a single ligation with transformation efficiency of over 5000 recombinants per microliter of ligation mixture. every clone in this library conta ... | 2000 | 10701119 |
| purification of a jojoba embryo fatty acyl-coenzyme a reductase and expression of its cdna in high erucic acid rapeseed. | the jojoba (simmondsia chinensis) plant produces esters of long-chain alcohols and fatty acids (waxes) as a seed lipid energy reserve. this is in contrast to the triglycerides found in seeds of other plants. we purified an alcohol-forming fatty acyl-coenzyme a reductase (far) from developing embryos and cloned the cdna encoding the enzyme. expression of a cdna in escherichia coli confers far activity upon those cells and results in the accumulation of fatty alcohols. the far sequence shows signi ... | 2000 | 10712526 |
| characterization of the brassica napus extraplastidial linoleate desaturase by expression in saccharomyces cerevisiae. | the substrate specificity and regioselectivity of the brassica napus extraplastidial linoleate desaturase (fad3) was investigated in vivo in a heterologous expression system. a strain of the yeast saccharomyces cerevisiae producing the plant enzyme was constructed and cultured in media containing a variety of fatty acids. the products of desaturation of these potential substrates were determined by gas chromatographic and mass spectrometric analysis of the yeast cultures. the results indicate th ... | 2000 | 10712534 |
| genetic engineering of glycinebetaine production toward enhancing stress tolerance in plants: metabolic limitations. | glycinebetaine (betaine) affords osmoprotection in bacteria, plants and animals, and protects cell components against harsh conditions in vitro. this and a compelling body of other evidence have encouraged the engineering of betaine production in plants lacking it. we have installed the metabolic step for oxidation of choline, a ubiquitous substance, to betaine in three diverse species, arabidopsis, brassica napus, and tobacco (nicotiana tabacum), by constitutive expression of a bacterial cholin ... | 2000 | 10712538 |
| digestion of fat does not differ in growing pigs fed diets containing fish oil, rapeseed oil or coconut oil. | we studied the digestion of fat and fatty acids in diets containing oils with different fatty acid composition. four barrows (initial weight 35 kg) were fitted with a simple t-cannula at the terminal ileum. three wheat starch and fish meal-based diets were formulated to contain either 150 g fish oil, rapeseed oil or coconut oil/kg. a basal diet, which did not contain oil, was also prepared. the diets were fed according to a 4 x 4 latin square design. each experimental period comprised 5 d adapta ... | 2000 | 10736341 |
| stimulation of the ionic transport system in brassica napus by a plant growth-promoting rhizobacterium (achromobacter sp.). | a plant growth-promoting rhizobacterium belonging to the genus achromobacter was isolated from the oil-seed-rape (brassica napus) root. growth promotion bioassays were performed with oilseed rape seedlings in a growth chamber in test tubes containing attapulgite and mineral nutrient solution, containing no3- as n source. the presence of this achromobacter strain increased shoot and root dry weight by 22-33% and 6-21%, respectively. inoculation of young seedlings with the achromobacter bacteria i ... | 2000 | 10749536 |
| compartmentation of atp:citrate lyase in plants. | extracts prepared from young leaves of pea (pisum sativum), tobacco (nicotiana tabacum), rape (brassica napus), and spinach (spinacia oleracea) all contained atp:citrate lyase (acl) activity, which was most active in rape leaflets (130 nmol min(-1) g fresh weight). in rape and spinach, acl activity was predominantly localized in the plastids (between about 78% and 90% of the total activity), whereas in pea and tobacco, distribution was mainly cytosolic (about 85% and 78%, respectively, of the to ... | 2000 | 10759519 |
| effects of glucosinolates and flavonoids on colonization of the roots of brassica napus by azorhizobium caulinodans ors571. | plants of brassica napus were assessed quantitatively for their susceptibility to lateral root crack colonization by azorhizobium caulinodans ors571(pxlgd4) (a rhizobial strain carrying the lacz reporter gene) and for the concentration of glucosinolates in their roots by high-pressure liquid chromatography (hplc). high- and low-glucosinolate-seed (hgs and lgs) varieties exhibited a relatively low and high percentage of colonized lateral roots, respectively. hplc showed that roots of hgs plants c ... | 2000 | 10788398 |
| inhibition by long-chain acyl-coas of glucose 6-phosphate metabolism in plastids isolated from developing embryos of oilseed rape (brassica napus l.). | the effects of long-chain acyl-coa (lcacoa) esters (both added exogenously and synthesized de novo) and acyl-coa binding protein (acbp) on plastidial glucose 6-phosphate (glc6p) and pyruvate metabolism were examined using isolated plastids. the binding of lcacoa esters by acbp stimulated the utilization of glc6p for fatty acid synthesis, starch synthesis and reductant supply via the oxidative pentose phosphate (opp) pathway. stimulation occurred at low (1-10 microm) concentrations of acbp. pyruv ... | 2000 | 10794725 |
| cloning, characterization and chromosomal location of three genes encoding host-cell-wall-degrading enzymes in leptosphaeria maculans, a fungal pathogen of brassica spp. | the ascomycete, leptosphaeria maculans, causes blackleg disease of oilseed brassica spp. such as canola (brassica napus). we have cloned a gene encoding endopolygalacturonase, pg1, and two genes encoding cellulases, cel1 and cel2, in l. maculans. these genes are not clustered in the genome, as they are located on different chromosomes. the deduced amino acid sequences of all three genes predict an n-terminal signal sequence, as is common for secreted fungal enzymes that degrade plant cell walls. ... | 2000 | 10806354 |
| dietary intake of rapeseed oil or soybean oil as the only fat nutrient in spontaneously hypertensive rats and wistar kyoto rats - blood pressure and pathophysiology. | spontaneously hypertensive rats (shr) and wistar kyoto (wky) rats were fed a diet containing 10% rapeseed (canola) oil or soybean oil as dietary fat, and given drinking water containing 1% nacl for 26 weeks. from the 10th week and later, systolic blood pressure in the canola oil group became higher than that in the soybean oil group in each strain. the 26-week feeding of canola oil increased plasma lipids and the neutrophil counts, and decreased the platelet counts. in the canola oil group the h ... | 2000 | 10814852 |
| influence of dietary fat and vitamin e supplementation on free radical production and on lipid and protein oxidation in turkey muscle extracts. | the objectives of this study were to investigate the effects of dietary fat (6% soy oil or rapeseed oil or tallow) and alpha-tocopheryl acetate supplementation at two levels (30 or 200 ppm) on radical production, measured by esr spectroscopy, and on lipid and protein oxidation in turkey muscle extracts oxidized by an enzymic system (nadph, adp, feso(4)/cytochrome p450 reductase). two muscles were tested: pectoralis major (glycolytic) and sartorius (oxidative) muscles. radical production measured ... | 2000 | 10820038 |
| pseudomonas brassicacearum sp. nov. and pseudomonas thivervalensis sp. nov., two root-associated bacteria isolated from brassica napus and arabidopsis thaliana. | bacteria isolates phenotypically related to pseudomonas corrugata have frequently been isolated from the rhizosphere of arabidopsis thaliana and brassica napus grown on different soils. 16s rdna (rrs) gene sequencing, dna-dna hybridization, biochemical characterization and siderophore typing showed that these isolates belong to two different species that are distinct from other species of the genus pseudomonas, including p. corrugata. a description of properties of these two new species is given ... | 2000 | 10826782 |
| lymphatic transport in rats of several dietary fats differing in fatty acid profile and triacylglycerol structure. | we examined in rats the intestinal absorption of nine very different dietary fats (two rapeseed oils, corn, olive, palm and menhaden oil, butter, cocoa butter and lard) to investigate the influence of fatty acid profile and triacylglycerol structure on absorption. absorption was followed for 24 h after administration of similar amounts of fats, and the accumulated lymphatic transport and amount of triacylglycerols found in lymph in response to the administered fats were calculated, revealing maj ... | 2000 | 10827219 |
| the methionine chain elongation pathway in the biosynthesis of glucosinolates in eruca sativa (brassicaceae). | glucosinolates are nitrogen- and sulfur-containing plant natural products that have become increasingly important in human affairs as flavor precursors, cancer-prevention agents, and crop protectants. while many glucosinolates are biosynthesized from common amino acids, the major glucosinolates in economically important species of the brassicaceae, such as brassica napus (oilseed rape), are thought to be formed from chain-elongated derivatives of methionine or phenylalanine. we investigated the ... | 2000 | 10860559 |
| salt adaptation in pseudomonads: characterization of glucosylglycerol-synthesizing isolates from brackish coastal waters and the rhizosphere. | the compatible solute glucosylglycerol (gg) is widespread among cyanobacteria, but, until now, has been reported for only two species of heterotrophic bacteria. about 120 bacterial isolates from coastal regions of the baltic sea were screened by hplc for their ability to synthesize gg. positive isolates (26) were grouped by sds-page of whole-cell proteins and representative strains of each group were investigated by sequencing their 16s rrna genes and phenotypic characterization. all gg-synthesi ... | 2000 | 10879976 |
| absorption and distribution of selenium in animals consuming canola grown for selenium phytoremediation. | canola (brassica napus) grown as a selected plant species for field phytoremediation of selenium (se) may be harvested and utilized as se-enriched forage for marginally se-deficient lambs and cows. two field studies were conducted under controlled conditions to evaluate the accumulation of se into different animal tissues, including blood, excreta, and milk. in study 1, treatments consisted of feeding lambs freshly cut se-enriched canola (containing approximately 4 mg se kg(-1) dm) or control ca ... | 2000 | 10903829 |
| accumulation and nuclear targeting of bnc24, a brassica napus ribosomal protein corresponding to a mrna accumulating in response to cold treatment. | previously, we described two cdna clones corresponding to a new family of cold regulated genes, pbnc24a and pbnc24b homologous to the human bbc1 (breast basic conserved) gene. in order to further analyze the function and regulation of these two genes we have prepared antibodies against a recombinant fusion protein, mbp-bnc24a and used them to study expression at the protein level. in contrast to the increased mrna accumulation induced by cold, immuno-blot analysis showed that the quantity of the ... | 2000 | 10908803 |
| different functions of vicilin and legumin are reflected in the histopattern of globulin mobilization during germination of vetch (vicia sativa l.). | the temporal and spatial patterns of storage-globulin mobilization were immunohistochemically pursued in the embryonic axis and cotyledons of vetch seed (vicia sativa l.) during germination and early seedling growth. embryonic axes as well as cotyledons of mature seeds contain protein bodies with stored globulins. prevascular strands of axes and cotyledons, the radicle and epidermal layers of axis organs were nearly exclusively stained by vicilin antibodies whereas the cotyledonous storage mesop ... | 2000 | 10923698 |
| importance of sequences adjacent to the terminal tripeptide in the import of a peroxisomal candida tropicalis protein in plant peroxisomes. | the peroxisome targeting signal (pts) required for import of the rat acyl-coa oxidase (aox; ec 1.3.3.6) and the candida tropicalis multifunctional protein (mfp) in plant peroxisomes was assessed in transgenic arabidopsis thaliana (l.) heynh. the native rat aox accumulated in peroxisomes in a. thaliana cotyledons and targeting was dependent on the presence of the c-terminal tripeptide s-k-l. in contrast, the native c. tropicalis mfp, containing the consensus pts sequence a-k-i was not targeted to ... | 2000 | 10923716 |
| thirteen-week dietary intake of rapeseed oil or soybean oil as the only dietary fat in wistar kyoto rats-change in blood pressure. | wistar kyoto (wky) rats were fed a diet containing 10% rapeseed (canola) oil or soybean oil as the only dietary fat for 13 weeks. from week 5 of feeding, systolic blood pressure of the canola oil group became higher than that of the soybean oil group. the 13-week canola oil intake increased plasma levels of na(+) and lipids, and decreased the level of k(+) compared to those in the soybean oil group. the canola oil group also showed a high density of neutrophils and a low density of platelets com ... | 2000 | 10930702 |
| influence of high light and uv-b radiation on photosynthesis and d1 turnover in atrazine-tolerant and -sensitive cultivars of brassica napus. | an atrazine-tolerant mutant and an atrazine-sensitive cultivar of brassica napus l. were grown under visible radiation (400 mumol m-2 s-1, photosynthetically active radiation, par) and then subjected to treatment conditions. these included short-term high par (1600 mumol m-2 s-1) which was given for 4 h either alone or in combination with an enhanced level of uv-bbe radiation (4.6 kj m-2 h-1 biologically effective uv-b, 280-320 nm). recovery from the radiation treatment was studied for 4 h under ... | 2000 | 10938832 |
| hplc analyses of cultures of phoma spp.: differentiation among groups and species through secondary metabolite profiles. | the metabolite profiles of 26 isolates of the blackleg fungus (leptosphaeria maculans (desm.) ces. et de not., asexual stage phoma lingam (tode ex fr.) desm.), obtained from diverse parts of the world (part of the international blackleg crucifer network collection), were studied utilizing specific culture conditions, hplc analysis, and a set of chemical markers. this fungus is the causative agent of blackleg disease of brassica oilseeds; a virulent strain of the pathogen has caused significant r ... | 2000 | 10941513 |
| intercodon dinucleotides affect codon choice in plant genes. | in this work, 710 cdss corresponding to over 290 000 codons equally distributed between brassica napus, arabidopsis thaliana, lycopersicon esculentum, nicotiana tabacum, pisum sativum, glycine max, oryza sativa, triticum aestivum, hordeum vulgare and zea mays were considered. for each amino acid, synonymous codon choice was determined in the presence of a, g, c or t as the initial nucleotide of the subsequent triplet; data were statistically analysed under the hypothesis of an independent assort ... | 2000 | 10954603 |
| mutagenicity of diesel exhaust particles from two fossil and two plant oil fuels. | particulate matter of diesel engine exhaust from four different fuels was studied for content of polynuclear aromatic compounds and mutagenic effects. two so-called biodiesel fuels, rapeseed oil methylesters (rme) and soybean oil methylesters (sme), were compared directly with two fossil diesel fuels with the normal (df) and a low sulfur content (ls-df). diesel exhaust particles were sampled on filters from the diluted and cooled exhaust of a test engine at five different speeds and loads. filte ... | 2000 | 10970444 |
| plants rendered herbicide-susceptible by cauliflower mosaic virus-elicited suppression of a 35s promoter-regulated transgene. | crop plants genetically modified for herbicide tolerance were some of the first to be released into the environment. frequently, the cauliflower mosaic virus (camv) 35s promoter is used to drive expression of the herbicide tolerance transgene. we analyzed the response to camv infection of a transgenic oilseed rape line containing the bialaphos tolerance gene (bar) from streptomyces hygroscopicus, regulated by the 35s promoter. oilseed rape is susceptible to camv, but plants recover from infectio ... | 2000 | 10973223 |
| a chimeric mini-trypsin inhibitor derived from the oil rape proteinase inhibitor type iii. | the design of chimeric proteins is a major field of interest in structural biology and biotechnology. the successful design of the chimeric protein composed by the minimized reactive site domain of the low-molecular-mass trypsin inhibitor from brassica napus (var. oleifera) seed (ser3-lys35; mini-rti-iii) and murine dihydrofolate reductase (dhfr) is reported here. the dhfr-mini-rti-iii chimeric protein was expressed in escherichia coli, purified by metal-chelate affinity chromatography and oxida ... | 2000 | 10973804 |
| [studies on transgenic oilseed rape (brassica napus) plants transformed with beta-1,3-glucanase and chitinase genes and its resistance to sclerotinia sclerotiorium]. | by agrobacterium-mediated method, the cotyledonary petiole of good quality rape variety h165 was transformed with plant expression vector pblgc which constitutively express beta-1,3-glucanase and chitinase genes. we obtained some kanamycin(kan)-resistant regenerational green shoots, with these shoots, pcr identification was conducted. results showed that 30% green shoots which grew in medium of kan 15 mg/l and 53% green shoots in kan 25 mg/l had positive reaction. we also made dot blot analysis ... | 2000 | 10976313 |
| [production of transgenic rape plants (brassica napus l.) using agrobacterium tumefaciens]. | the procedure for genetic transformation of two spring and one winter rapeseed cultivars was developed. no-paline strains of agrobacterium tumefaciens gv3101 and eha105 were shown to be preferable for gene transfer, as compared to the octopine strain gv2260. with two types of plant explants, the segments of hypocotyls and cotyledons, transformation was successful; however, its efficiency was somewhat higher with the fragments of hypocotyls. analysis of regenerated plants by pcr and southern blot ... | 2000 | 10994497 |
| human acylation stimulating protein enhances triacylglycerol biosynthesis in plant microsomes. | diacylglycerol acyltransferase has a universal role in catalyzing the acyl-coa-dependent formation of triacylglycerol in microorganisms, animals and plants. acylation stimulating protein, from human blood, is known to enhance diacylglycerol acyltransferase activity and triacylglycerol biosynthesis in human adipocytes. in the current study, acylation stimulating protein was also shown to enhance diacylglycerol acyltransferase activity in microsomes from cell suspension cultures of oilseed rape. e ... | 2000 | 10996321 |
| blood coagulation and osmolar tolerance of erythrocytes in stroke-prone spontaneously hypertensive rats given rapeseed oil or soybean oil as the only dietary fat. | the life-span of stroke-prone spontaneously hypertensive rats (shrsp) has been reported to become shorter by ingestion of some vegetable oils, including rapeseed oil, when given as the sole dietary fat. the present study was undertaken to examine if the ingestion of rapeseed (canola) oil affects blood coagulating time and erythrocyte membranes. namely, shrsp were orally given canola oil or soybean oil as the only dietary fat (10% of diet) for 4 weeks. after the 4-week feeding, activated partial ... | 2000 | 10996482 |
| novel bacterial diversity recovered from the rhizosphere of oilseed rape (brassica napus) determined by the analysis of 16s ribosomal dna. | soil was sampled to a distance of 2.5 mm beneath a root mat of oilseed rape (brassica napus) in a model rhizosphere system. dna was extracted and the 16s rdna amplified, cloned and sequenced. phylogenetic analysis of these sequences with those held on-line, revealed that 37% of the clones fell within the holophaga /acidobacterium phylum, 17% were within the proteobacteria, 14% of the clones were close relatives of bacillus megaterium and 5% were related to verrucomicrobium spinosum. an additiona ... | 2000 | 11016691 |
| lung cancer and indoor air pollution in rural china. | purpose: indoor air pollution has been linked with lung cancer in china. in contrast to previous studies conducted in urban areas with high levels of industrial pollution, we undertook a lung cancer case-control study in a rural area of china, where residents live in underground dwellings. we evaluated the effects of radon, wood and coal combustion, cooking oil fumes, and environmental tobacco smoke on lung cancer risk.methods: we enrolled 886 lung cancer cases (656 males, 230 females) diagnosed ... | 2000 | 11018397 |
| characterization of five new low-molecular-mass trypsin inhibitors from white mustard (sinapis alba l.) seed. | five new low-molecular-mass trypsin inhibitors belonging to the rti/mti-2 family were identified from white mustard (sinapis alba l. ; mti-2) seed. purified mti-2 consisted of a peptide mixture, displaying ile or arg at position 43, trp or kynurenine (kyn) at position 44, and c-terminal ragged ends. the occurrence of ile or arg at position 43 suggested that mti-2 inhibitors originated from different genes. the presence of 5-oxo-proline (pyroglutamic acid; 5-oxopro1) and kyn44 reflected post-tran ... | 2000 | 11029593 |
| transactivation of the brassica napus napin promoter by abi3 requires interaction of the conserved b2 and b3 domains of abi3 with different cis-elements: b2 mediates activation through an abre, whereas b3 interacts with an ry/g-box. | the transcriptional activator abi3 is a key regulator of gene expression during embryo maturation in crucifers. in monocots, the related vp1 protein regulates the em promoter synergistically with abscisic acid (aba). we identified cis-elements in the brassica napus napin napa promoter mediating regulation by abi3 and aba, by analyzing substitution mutation constructs of napa in transgenic tobacco plantlets ectopically expressing abi3. in transient analysis using particle bombardment of tobacco l ... | 2000 | 11029704 |
| cholesterol-lowering potential in human subjects of fat from pigs fed rapeseed oil. | the possibility of achieving blood-lipid-lowering characteristics of pig fat by increasing the content of unsaturated fat in pig feed was evaluated. three pig feeding regimens were applied: basal feed (no added fat or vitamin e), basal feed + rapeseed oil (60 g/kg feed), and basal feed + rapeseed oil (60 g/kg) + vitamin e (200 mg/kg). meat and meat products from the three pig groups were incorporated into diets providing 86 g pig fat/10 mj. the diets were served to twelve healthy human male subj ... | 2000 | 11029964 |
| the cylindrical inclusion gene of turnip mosaic virus encodes a pathogenic determinant to the brassica resistance gene turb01. | the viral component of turnip mosaic virus (tumv) determining virulence to the brassica napus turb01 dominant resistance allele has been identified. sequence comparisons of an infectious cdna clone of the uk 1 isolate of tumv (avirulent on turb01) and a spontaneous mutant capable of infecting plants possessing turb01 suggested that a single nucleotide change in the cylindrical inclusion (ci) protein coding region (gene) of the virus was responsible for the altered phenotype. a second spontaneous ... | 2000 | 11043471 |
| structured lipids improve fat absorption in normal and malabsorbing rats. | the presence of medium-chain fatty acids in dietary fatty acid as well as the triacylglycerol structure may influence the absorption and lymphatic transport of fatty acids. we compared the lymphatic transport and recovery of fatty acids from four intragastrically administered fats based on rapeseed oil and decanoic acid in two rat models of normal absorption and malabsorption, respectively. the fats were: 1) a fat with a regiospecific structure, 2) a similar fat but with a random distribution of ... | 2000 | 11053524 |
| increase in blood pressure with enhanced na+, k+ -atpase activity in stroke-prone spontaneously hypertensive rats after 4-weeks intake of rapeseed oil as the sole dietary fat. | twenty stroke-prone spontaneously hypertensive rats were divided into 2 groups of 10 animals each, and fed a defatted diet and orally administered rapeseed (canola) oil or soybean oil at 10 (w/w)% of the consumed diet once a day for 4 weeks. at the 4th week of administration, the systolic blood pressure in the canola oil group was higher (235 +/- 2 mmhg, mean +/- s.e.m., n=10) than that in the soybean oil group (225 +/- 4 mmhg, n=10, p<0.05). in isolated, perfused mesenteric bed from these rats, ... | 2000 | 11068856 |
| cytotoxic and mutagenic effects, particle size and concentration analysis of diesel engine emissions using biodiesel and petrol diesel as fuel. | diesel engine exhaust particles (dep) contribute substantially to ambient air pollution. they cause acute and chronic adverse health effects in humans. biodiesel (rapeseed oil methyl ester. rme) is used as a "green fuel" in several countries. for a preliminary assessment of environmental and health effects of rme, the particulate-associated emissions from the dep of rme and common fossil diesel fuel (df) and their in vitro cytotoxic and mutagenic effects were compared. a test tractor was fuelled ... | 2000 | 11097388 |
| combined effects of brassica napus seed meal and trichoderma harzianum on two soilborne plant pathogens. | the effects of soil amendment with rapeseed meal from brassica napus cv. 'dwarf essex' (high glucosinolate concentrations) and 'stonewall' (low glucosinolate concentrations) on the biological control activity of trichoderma harzianum towards sclerotinia sclerotiorum and aphanomyces euteiches were evaluated. trichoderma harzianum added to soil reduced myceliogenic germination of s. sclerotiorum by 94%, but did not affect carpogenic germination. in contrast, 100% reduction in carpogenic germinatio ... | 2000 | 11109495 |
| milk fat conjugated linoleic acid (cla) inhibits growth of human mammary mcf-7 cancer cells. | the relationship between growth and the antioxidant enzyme defence system in human mcf-7 (breast) cancer cells treated with bovine milk fat enriched with conjugated linoleic acid (cla) was studied. milk enriched in cla was obtained from cows on pasture supplemented with full fat rapeseeds and full fat soyabeans (1). cell number decreased up to 90% (p < 0.05) and lipid peroxidation increased 15-fold (p < 0.05) following incubation of mcf-7 cells for 8 days with increasing levels of milk fat yield ... | 2000 | 11131667 |
| effects of season and processing on oil content and fatty acids of baltic herring (clupea harengus membras). | fatty acid composition, oil content, free fatty acid content, and peroxide value of baltic herring (clupea harengus membras) and two processed products (fried fillets and fish burgers) were investigated. the highest oil content of the fillets was found in autumn (10%), at the time when the free fatty acids had their minimum (1.4%). the main fatty acids were oleic (18-23%), palmitic (17%), palmitoleic (8-12%), and docosahexaeneoic (8-10%) acids. the proportion of saturated fatty acids was a const ... | 2000 | 11141273 |
| cloning and heterologous expression of a rape cdna encoding udp-glucose:sinapate glucosyltransferase. | a cdna encoding a udp-glucose:sinapate glucosyltransferase (sgt) that catalyzes the formation of 1-o-sinapoylglucose, was isolated from cdna libraries constructed from immature seeds and young seedlings of rape (brassica napus l.). the open reading frame encoded a protein of 497 amino acids with a calculated molecular mass of 55,970 da and an isoelectric point of 6.36. the enzyme, functionally expressed in escherichia coli, exhibited broad substrate specificity, glucosylating sinapate, cinnamate ... | 2000 | 11144274 |
| in planta sequential hydroxylation and glycosylation of a fungal phytotoxin: avoiding cell death and overcoming the fungal invader. | to facilitate plant colonization, some pathogenic fungi produce phytotoxic metabolites that damage tissues; plants may be resistant to a particular pathogen if they produce an enzyme(s) that catalyzes detoxification of this metabolite(s). alternaria blackspot is one of the most damaging and significant fungal diseases of brassica crops, with no source of resistance known within the brassica species. destruxin b is the major phytotoxin produced by the blackspot-causing fungus, alternaria brassica ... | 2001 | 11149945 |
| maintaining methylation activities during salt stress. the involvement of adenosine kinase. | synthesis of the compatible osmolyte gly betaine is increased in salt-stressed spinach (spinacia oleracea). gly betaine arises by oxidation of choline from phosphocholine. phosphocholine is synthesized in the cytosol by three successive s-adenosyl-met-dependent n-methylations of phosphoethanolamine. with each transmethylation, a molecule of s-adenosylhomo-cys (sah) is produced, a potent inhibitor of s-adenosyl-met-dependent methyltransferases. we examined two enzymes involved in sah metabolism: ... | 2001 | 11161043 |
| overexpression of 3-ketoacyl-acyl-carrier protein synthase iiis in plants reduces the rate of lipid synthesis. | a cdna coding for 3-ketoacyl-acyl-carrier protein (acp) synthase iii (kas iii) from spinach (spinacia oleracea; so kas iii) was used to isolate two closely related kas iii clones (ch kas iii-1 and ch kas iii-2) from cuphea hookeriana. both ch kas iiis are expressed constitutively in all tissues examined. an increase in the levels of 16:0 was observed in tobacco (nicotiana tabacum, wt-sr) leaves overexpressing so kas iii when under the control of the cauliflower mosaic virus-35s promoter and in a ... | 2001 | 11161065 |
| substrate specificity, regioselectivity and cryptoregiochemistry of plant and animal omega-3 fatty acid desaturases. | in order to define the substrate requirements, regiochemistry and cryptoregiochemistry of the omega-3 fatty acid desaturases involved in polyunsaturated fatty acid formation, the genes fad3 and fat-1 from brassica napus and the nematode caenorhabditis elegans respectively were expressed in baker's yeast (saccharomyces cerevisiae). various fatty acids, including deuterium-labelled thia-fatty acids, were supplied to growing cultures of transformed yeast. the results from gc-ms analysis of the desa ... | 2000 | 11171151 |
| further characterization of delta(8)-sphingolipid desaturases from higher plants. | a previously cloned cdna from helianthus annuus codes for a fusion protein composed of an n-terminal cytochrome b(5) and a c-terminal desaturase domain. for a functional identification, this cdna was expressed in saccharomyces cerevisiae and the structures of sphingolipid long-chain bases were analysed. the expression of this sunflower enzyme resulted in the formation of new delta(8)-trans/cis-phytosphingenine from c(18)- and c(20)-phytosphinganine present in wild-type yeast cells. to elucidate ... | 2000 | 11171153 |
| utility of the arabidopsis fae1 and yeast slc1-1 genes for improvements in erucic acid and oil content in rapeseed. | high-erucic acid (hea) brassica napus cultivars are regaining interest in industrial contexts. erucic acid and its derivatives are important renewable raw materials utilized in the manufacture of plastic films, in the synthesis of nylon 13,13, and in the lubricant and emollient industries. theoretically, the highest level of erucic acid that can be achieved by means of classical breeding is 66 mol%; however, using new approaches on the basis of genetic engineering, it might be possible to develo ... | 2000 | 11171262 |
| high-oleic acid australian brassica napus and b. juncea varieties produced by co-suppression of endogenous delta12-desaturases. | genetic engineering methods have been used successfully to modify the fatty acid profile of elite australian germplasm of brassica napus and b. juncea. co-suppression plasmids carrying oleate desaturase genes from each species have been constructed and transferred into australian elite breeding lines of b. napus and b. juncea using agrobacterium tumifaciens plant-transformation techniques. modifications to existing brassica transformation protocols and the use of an intron-interrupted hygromycin ... | 2000 | 11171263 |
| evidence of cross-reactivity between porcine pancreatic and rapeseed (brassica napus l.) lipases. | a cross-reactivity between animal and plant lipases was determined, using immunological techniques. it was shown by elisa and dot-blotting that these antibodies react with lipases in the rapeseed crude extract and in the different cellular fractions obtained by differential centrifugation. pre-incubation of the antiserum with the rapeseed crude extract affects the amount of antibodies binding to the porcine pancreatic lipase. antibodies were able to precipitate lipase activity from 3-day-old rap ... | 2000 | 11171277 |
| contribution of blotting techniques to the study of rapeseeds (brassica napus l.) lipases. | a recent advance in the study of plant lipases involving immunological techniques is presented. in an attempt to characterize lipases of cotyledons from germinating rapeseed seedlings and to investigate an eventual cross-reactivity with animal lipases, we have prepared anti-porcine pancreatic lipase antibodies raised in rabbit. it is shown by enzyme-linked immunosorbent assay and dot-blotting that these antibodies react with lipases in the rapeseed crude extract and in the different cellular fra ... | 2001 | 11197170 |
| influence of dietary fat source and copper supplementation on broiler performance, fatty acid profile of meat and depot fat, and on cholesterol content in meat. | 1. three hundred and twenty d-old chickens were fed on a wheat/maize-soyabean meal diet supplemented with (i) 50 g/kg lard, (ii) 25 g/kg lard and 25 g/kg rapeseed oil, (iii) 50 g/kg rapeseed oil, and (iv) 50 g/kg rapeseed oil and 200 mg copper per kg as copper sulphate pentahydrate. 2. final weights at 39 d of age in chickens receiving rapeseed oil were lower by 9% than in those fed on the diet containing only lard (p<0.05). the fatty acids profiles of lipids extracted from the tissues of 10 chi ... | 2000 | 11201441 |
| triclosan inhibits the growth of plasmodium falciparum and toxoplasma gondii by inhibition of apicomplexan fab i. | fab i, enoyl acyl carrier protein reductase (enr), is an enzyme used in fatty acid synthesis. it is a single chain polypeptide in plants, bacteria, and mycobacteria, but is part of a complex polypeptide in animals and fungi. certain other enzymes in fatty acid synthesis in apicomplexan parasites appear to have multiple forms, homologous to either a plastid, plant-like single chain enzyme or more like the animal complex polypeptide chain. we identified a plant-like fab i in plasmodium falciparum ... | 2001 | 11239932 |
| effects of preterm infant formula supplementation with alpha-linolenic acid with a linoleate/alpha-linolenate ratio of 6: a multicentric study. | to investigate the effects of a milk formula supplemented with a alpha-linolenic acid (ala) (18:2 n-6/18:3 n-3 ratio near 6/1) on plasma and red blood cell (rbc) fatty acids (fas) in premature infants and compare with a non supplemented formula (18:2 n-6/18:3 n-3 = 22/1). | 1997 | 11248877 |
| storage time and deodorization temperature influence the formation of aniline-derived compounds in denatured rapeseed oils. | in 1981 an epidemic, named toxic oil syndrome, occurred in spain as a result of ingestion of rapeseed oil denatured with 2% aniline, which had been imported for industrial use but was fraudulently diverted and processed for human consumption. two groups of chemical compounds have been identified in the ingested toxic oil: fatty acid anilides and amino-propanediol derivatives. the objective of this work was to assess the effect of several refining process variables on the formation of 3-(n-phenyl ... | 2001 | 11259855 |
| effects of a mustard trypsin inhibitor expressed in different plants on three lepidopteran pests. | the effects of mustard trypsin inhibitor mti-2 expressed at different levels in transgenic tobacco, arabidopsis and oilseed rape lines have been evaluated against three different lepidopteran insect pests. 1. plutella xylostella (l.) larvae were the most sensitive to the ingestion of mti-2. the inhibitor expressed at high levels in arabidopsis plants caused rapid and complete mortality. high mortality and significantly delayed larval development were also detectable in oilseed rape expressing mt ... | 2001 | 11267898 |
| molecular characterization of the host-adapted pathogen verticillium longisporum on the basis of a group-i intron found in the nuclear ssu-rrna gene. | verticillium wilt of oilseed rape is caused by the host-adapted pathogen verticillium longisporum comb. nov. with one set of nuclear ssu-rrna gene primers, a pcr amplification product of ca. 2.5 kb was generated from all isolates of v. longisporum tested (36 from europe, japan, and usa), with the exception of two recombinant isolates. on the contrary, all the other phytopathogenic and nonphytopathogenic species of verticillium tested (18 species, 46 isolates), with the exception of one isolate o ... | 2001 | 11277099 |
| brassicaceae express multiple isoforms of biotin carboxyl carrier protein in a tissue-specific manner. | plastidial acetyl-coenzyme a carboxylase from most plants is a multi-enzyme complex comprised of four different subunits. one of these subunits, the biotin carboxyl carrier protein (bccp), was previously proposed to be encoded by a single gene in arabidopsis. we report and characterize here a second arabidopsis bccp (atbccp2) cdna with 42% amino acid identity to atbccp1 and 75% identity to a class of oilseed rape (brassica napus) bccps. both arabidopsis bccp isoforms were expressed in escherichi ... | 2001 | 11299381 |
| effect of randomization of mixtures of butter oil and vegetable oil on absorption and lipid metabolism in rats. | the nutritional effect of the regiospecific distribution of fatty acids in edible fats is currently discussed due to an increased use of interesterification of fats for human consumption. however, disagreeing results have been reported which may be due to the varying composition of the dietary fats compared. data on the fate of such lipids beyond the bloodstream is rather scarce and animal model studies are needed. | 2001 | 11315499 |
| major human plasma lipid classes determined by quantitative high-performance liquid chromatography, their variation and associations with phospholipid fatty acids. | an hplc method with evaporative light-scattering detection (elsd) was optimized and validated for the simultaneous quantitation of cholesteryl esters (ces), triacylglycerols (tgs), free cholesterol (fc) and phosphatidylcholine (pc) in human plasma. the separation of ces from tgs, the most variable plasma lipid class, was improved by speeding up the gradient steps and by increasing the re-equilibration time between runs. the calibrations were made at levels of 0.14-14 microg lipid/injection. the ... | 2001 | 11339287 |
| replacement of fish oil with rapeseed oil in diets of atlantic salmon (salmo salar) affects tissue lipid compositions and hepatocyte fatty acid metabolism. | duplicate groups of atlantic salmon post-smolts were fed five practical-type diets in which the added lipid was 100% fish oil [fo; 0% rapeseed oil (0% ro)], 90% fo + 10% ro (10% ro), 75% fo + 25% ro (25% ro), 50% fo + 50% ro (50% ro) or 100% ro, for a period of 17 wk. there were no effects of diet on growth rate or feed conversion nor were any histopathological lesions found in liver, heart, muscle or kidney. the greatest accumulation of muscle lipid was in fish fed 0% ro, which corresponded to ... | 2001 | 11340112 |
| overexpression of the tobacco tsi1 gene encoding an erebp/ap2-type transcription factor enhances resistance against pathogen attack and osmotic stress in tobacco. | using mrna differential display analysis, we isolated a salt-induced transcript that showed a significant sequence homology with an erebp/ap2 dna binding motif from oilseed rape plants. with this cdna fragment as a probe, cdna clone tsi1 (for tobacco stress-induced gene1) was isolated from a tobacco cdna library. rna gel blot analysis indicated that transcripts homologous with tsi1 were induced not only in nacl-treated leaves but also in leaves treated with ethephon or salicylic acid. transient ... | 2001 | 11340180 |
| isolation and characterization of a d-7 lea protein from pollen that stabilizes glasses in vitro. | a heat-soluble protein present in substantial quantities in typha latifolia pollen was purified to homogeneity. the protein was subjected to cyanogen bromide cleavage, and the peptides produced were separated by hplc chromatography and sequenced. the two sequences determined were found to be related to the putative d76 lea protein from brassica napus seeds and one of them to the d-7 lea protein from upland cotton. this suggests the pollen protein to be a member of the lea group iii family of pro ... | 2001 | 11341929 |