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non-gonococcal urethritis in men & its response to therapy. 19846097537
[microbiologic findings in vaginal discharges].microbiological examinations were performed in 247 women complaining of vaginal discharge. an average of 3.9 different organisms was isolated from each patient. gardnerella vaginalis was found in 62.8% of all women. candida spp. were seen in 22.7%. trichomonas vaginalis occurred in 6.5%, neisseria gonorrhoeae in 1.2%, chlamydia trachomatis in 10.1%, mycoplasmata in 13.8%, cytomegalovirus in 2.8%, and herpes simplex type 2-virus in 1.1%. none of the above was found in 17.4% of the patients. stati ...19846098512
corynebacterium vaginale and vaginitis: a controlled trial of treatment.in a study of the diagnosis and treatment of corynebacterium vaginale (haemophilus vaginalis) vaginitis in 30 patients, clinical and microscopical findings were compared with laboratory cultures. the study also included a double-blind randomised trial of treatment regimens including placebo therapy. laboratory cultures of c. vaginale corresponded well with clinical findings, and we suggest that c. vaginale vaginitis can be reliably diagnosed with clinical and microscopical findings. tetracycline ...19806102231
metronidazole for non-specific vaginitis. 19806103353
metronidazole for non-specific vaginitis. 19806103355
tetracycline and corynebacterium vaginale. 19806103471
metronidazole for corynebacterium vaginale vaginitis. 19806105324
metronidazole metabolite and gardnerella vaginalis (corynebacterium vaginale) 19816112482
gardnerella vaginalis and prostatitis. 19816116022
sensitivity of gardnerella vaginalis to metabolites of metronidazole and tinidazole. 19826119542
comparison of bacterial and fungal adherence to vaginal exfoliated epithelial cells and human vaginal epithelial tissue culture cells.the adherence of four bacterial species and candida albicans to a new in vitro tissue culture model of human vaginal stratified squamous epithelium was investigated and compared with in vitro adherence to vaginal exfoliated cells. gardnerella vaginalis, group b streptococci, lactobacillus sp., and c. albicans adhered well to both exfoliated and tissue culture cells. similarly, a piliated fecal isolate of escherichia coli, but not a nonpiliated vaginal isolate of e. coli, adhered well to both cel ...19826120141
gardnerella vaginalis, anaerobes, and vaginal discharge. 19826123676
gardnerella vaginalis: pathogen or commensal? 19836134952
gardnerella vaginalis. 19836135858
anaerobic vaginosis (non-specific vaginitis): clinical, microbiological, and therapeutic findings.the effect of metronidazole on anaerobic vaginosis (non-specific vaginitis) was assessed in a double-blind, placebo-controlled study of 40 women. 19 of 20 women given metronidazole 400 mg twice a day for seven days were clinically and microbiologically cured by the time they completed treatment. all 20 women given a placebo were treatment failures, but when they were given a single 2 g dose of metronidazole, 15 showed clinical and microbiological cure a week later. 14 of the responders showed a ...19836140492
single dose versus seven day metronidazole in gardnerella vaginalis associated non-specific vaginitis. 19846145055
metronidazole in treatment of anaerobic vaginosis. 19846145994
demonstration by electron microscopy of pili on gardnerella vaginalis.eight strains of gardnerella vaginalis were examined by electron microscopy for the presence of pili. narrow pili ranging from 3.0 to 7.5 nm in diameter were seen on bacteria from five of the strains studied.19846151415
haemophilus vaginalis infection. diagnosis and treatment.some 4,263 women from special, family planning and gynecologic clinics were screened at random for the presence of h. vaginalis (c. vaginale) infection. twenty-four percent of the women from the special clinics, 6% from the family planning and 4% from the gynecology clinics were culture positive although microscopic findings were somewhat higher than this. of 582 women attending special clinics who were infected solely with h. vaginalis, only 261 complained of having an offensive discharge. two ...19806153721
diagnosis of bacterial vaginosis by direct gram stain of vaginal fluid.to determine whether bacterial vaginosis (bv), also known as nonspecific vaginitis, could be diagnosed by evaluating a gram stain of vaginal fluid, we examined samples from 60 women of whom 25 had clinical evidence of bv and 35 had candidal vaginitis or normal examinations. an inverse relationship between the quantity of the lactobacillus morphotype (large gram-positive rods) and of the gardnerella morphotype (small gram-variable rods) was noted on gram stain (p less than 0.001). when gram stain ...19836193137
in vitro activity of seventeen antimicrobial agents against gardnerella vaginalis.the in vitro activity of 17 antimicrobial agents was tested against 25 clinical isolates of gardnerella vaginalis. minimum inhibitory concentrations were determined by agar dilution. the isolates were sensitive to penicillin, ampicillin, ticarcillin, piperacillin, cephalothin, cefoxitin, cefotaxime, cefoperazone, n-formimidoyl-thienamycin, chloramphenicol, clindamycin and erythromycin. mic90 for the beta-lactam antibiotics ranged from 0.12 mg/l for penicillin to 2 mg/l for ticarcillin. cefoperaz ...19826223813
[first experience with the diagnosis of gardnerella vaginalis]. 19846230162
in vitro activity of ciprofloxacin and norfloxacin against gardnerella vaginalis. 19846237906
vaginitis in sexually active women: relationship to nine sexually transmitted organisms.women seen for symptoms suggestive of vulvovaginitis were studied for the detection of mycoplasma hominis. ureaplasma urealyticum, yeast, neisseria gonorrhoeae, chlamydia trachomatis, gardnerella vaginalis, herpes simplex virus, group b beta-hemolytic streptococci, aerobes, anaerobes, and trichomonas vaginalis. asymptomatic women who reported to be sexually active and agreed to undergo comprehensive genital cultures were used as controls. there was a significant association of vulvovaginitis wit ...19826280502
polymicrobial nature of vaginitis in young women: a microbiological and therapeutic study.thirty-six young females attending the student health service with vaginitis were investigated by serial semiquantitative aerobic, anaerobic, fungal, mycoplasma and viral cultures over a 10 day period and results were correlated with signs and symptoms. antifungal therapy (econazole pessaries and cream) resulted in clearance of candida from 13 out of 16 patients where there was no increase in the anaerobic flora. in the four subjects where candida was isolated along with gardnerella vaginalis pl ...19826283441
upper urinary tract infection with gardnerella vaginalis in a woman. 19846332020
microbiology of vaginitis associated with the intrauterine contraceptive device.a malodorous, homogeneous, grey, thin and non-purulent discharge, which resembles non-specific vaginitis occurs in 20% of women with an intrauterine contraceptive device (iucd); four times more common than in non-users. the aim of this investigation was to study the bacteriological aetiology of this iucd-associated vaginal discharge, and to assess whether the infection was ascendent. no specific microbiological aetiology was found, but the normal, lactobacillus-dominated microbial vaginal flora ...19846332643
biotypes of gardnerella vaginalis.a simple and reproducible scheme for identifying biotypes of gardnerella vaginalis has been developed, based on reactions for lipase, hippurate hydrolysis, and beta-galactosidase. among a total of 359 strains tested, eight biotypes were observed, the most common ones being types 1 (beta-galactosidase positive, lipase positive, hippurate positive), 2 (beta-galactosidase negative, lipase positive, hippurate positive), and 5 (beta-galactosidase negative, lipase negative, hippurate positive). the di ...19846333436
treatment of gardnerella vaginalis vaginitis. 19846333920
anaerobic vaginosis: therapeutic and epidemiological aspects. 19846334683
use of betadine in the pregnant woman. 19846334771
susceptibility of gardnerella vaginalis to thiamphenicol: clinical experience with nonspecific vaginitis.we compared the in-vitro activity of thiamphenicol against 100 strains of gardnerella vaginalis with the activity of 11 other antimicrobial agents. the mics for thiamphenicol ranged from 0.39 micrograms/ml to 6.25 micrograms/ml. the concentration at which 50% of strains were inhibited (mic50) was 1.96 micrograms/ml, and the concentration at which 90% of strains were inhibited (mic90) was 3.93 micrograms/ml. all strains were very susceptible to erythromycin, chloramphenicol, beta-lactam antibioti ...19846335301
a newly discovered sialidase from gardnerella vaginalis.a sialidase (neuraminidase, acylneuraminosyl hydrolase, ec 3.2.1.18) has been discovered and isolated from gardnerella vaginalis (ex. haemophilus vaginalis), a possibly pathogenic inhabitant of the female genital tract. bacteria were grown in peptone-yeast-extract medium with 2.0 mm n-acetylmannosamine as enzyme inductor under co2 atmosphere. sialidase activity was found in the bacterial sediment and in the culture medium. the enzyme was liberated from the cells by ultrasonic treatment. purifica ...19846335332
experimental infection of mares with haemophilus equigenitalis.inoculation of haemophilus equigenitalis into the uterus of 7 mares caused a disease clinically indistinguishable from contagious equine metritis. the duration of clinical signs varied from 4 to 11 days. the causative organism persisted for a relatively short time (2 to 10 weeks) in 5 mares, but in 2 others it established a carrier status and persisted until they were killed 6 and 10 months after infection. h. equigenitalis was recovered from the vestibule of the vagina and from a combined swab ...19846335966
clinical aspects of gardnerella vaginalis-associated vaginitis. a review of the literature.almost 30 years ago gardner & dukes introduced the term haemophilus vaginalis vaginitis. most authors agree that this disease entity is characterized by a malodorous, homogeneous, greyish-white discharge, displaying clue cells in the wet-mount preparation. the diagnosis can be made in the office, the microscope being the most important diagnostic tool. there still seems to be some controversy regarding cause and clinical manifestations of the condition. a lack of uniformity in case definition an ...19846336130
vaginal colonization with gardnerella vaginalis and anaerobic curved rods.ninety-four women colonized with anaerobic curved rods (cr) and/or gardnerella vaginalis, were studied. of these women, 34 were culture-positive for the long variant of cr. eight of these latter women also harboured the short variant of these bacteria. in women colonized with cr, none had a predominance of lactobacilli in their vaginal flora, which was the case in 26 of the 60 women not harbouring cr. g. vaginalis was isolated from all 94 women. mycoplasma hominis occurred significantly more oft ...19846336131
studies on the mechanism of adhesion of gardnerella vaginalis to human erythrocytes.a quantitative method using radiolabelled bacteria was used to examine the adherence of gardnerella vaginalis to human erythrocytes. adherence was mannose resistant, but inhibited by d-galactose and d-galactosamine. it was independent of temperature, but reduced at ph 3. there was considerable variation in the adherence of different isolates of g. vaginalis to human erythrocytes.19846336132
can gardnerella vaginalis and anaerobic curved rods attach to vaginal epithelial cells in vitro, resulting in clue cells?vaginal epithelial cells were taken from asymptomatic patients and from patients with non-specific vaginosis. four bacterial species-gardnerella vaginalis, wolinella succinogenes and short and long anaerobic curved rods-were tested in vitro regarding attachment to the epithelial cells. in most experiments the curved rods and w. succinogenes attached to the cells. less often, incubation with g. vaginalis resulted in cell attachment. there was no difference in attachment ability between the short ...19846336133
the grivet monkey as a model for study of vaginitis. challenge with anaerobic curved rods and gardnerella vaginalis.the grivet monkey was used as an experimental model for vaginitis. gardnerella vaginalis was used for challenge in one monkey. g. vaginalis plus a long variant (lcr) of a hitherto unclassified anaerobic curved rod (cr) in another 2 monkeys, this lcr alone in 2, a short variant (scr) of cr in 2, and g. vaginalis plus scr in one monkey. all the bacterial strains had been isolated from human vaginal specimens. in the 2 monkeys exposed to g. vaginalis plus lcr, distinct signs of bacterial vaginosis ...19846336134
vaginal colonization of pig-tailed macaques by gardnerella vaginalis.ten pig-tailed macaques inoculated intravaginally with gardnerella vaginalis organisms were colonized for 11-39 days. in contrast, 4 tamarins and 3 chimpanzees inoculated similarly failed to become colonized. examination of gram-stained vaginal smears obtained from infected pig-tailed macaques failed to demonstrate clue cells, a feature which is pathognomonic of non-specific vaginitis in humans. additionally, the ph value, the levels of non-volatile fatty acids and the anaerobic flora of the mac ...19846336135
bacterial vaginosis during pregnancy. an association with prematurity and postpartum complications.although bacterial vaginosis (bv) is a common disorder associated with high concentrations of potentially pathogenic micro-organisms, bv has not yet been linked to infections outside the vagina. to investigate the association of bv with adverse outcomes during or following pregnancy, we analysed the prevalence of bv among women with and without premature labor, and early-onset postpartum endometritis. bacterial vaginosis was identified by gas-liquid chromatographic criteria in 28 (49%) of 57 wom ...19846336136
bacterial vaginosis. an evaluation of treatment.various treatment regimens for bacterial vaginosis are reviewed. orally administered metronidazole and tinidazole have been consistently successful, whereas widely variable results have been published from use of peroral ampicillin or tetracycline and from locally applied sulphonamide cream. the rationale for different treatments is discussed, with particular regard to the pathogenesis of the disease.19846336137
gynatren/solcotrichovac in the treatment of non-specific vaginitides. 19846336151
treatment of non-specific colpitis with gynatren/solcotrichovac. 19846336152
comparison of mid catheter collection and suprapubic aspiration of urine for diagnosing bacteriuria due to fastidious micro-organisms.we examined 40 female patients for the presence of fastidious bacteria in the bladder by culture of mid catheter and suprapubic aspiration urine specimens. gardnerella vaginalis and ureaplasma urealyticum were the most frequently isolated fastidious species. the suprapubic aspirate was positive in 3 patients with more than 10(5) colony-forming units per ml. gardnerella vaginalis and 7 with more than 10(2) colony-forming units per ml. ureaplasma urealyticum in the mid catheter urine specimen, whi ...19836338252
detection of gardnerella vaginalis on vaginal smears by immunofluorescence.an indirect fluorescence antibody (ifa) test was developed for the detection of gardnerella vaginalis. antisera were prepared in rabbits by using five strains of g. vaginalis. a pool of the antisera was tested for specificity with a variety of isolates known to colonize the human vagina and (or) morphologically resemble g. vaginalis. six heterologous bacterial isolates reacted with the pooled antiserum at dilutions of 1:10, but none reacted at the working dilution of 1:200. vaginal swab specimen ...19836339017
single oral dose metronidazole therapy for gardnerella vaginalis vaginitis in adolescent females.this study briefly reviews gardnerella vaginalis as a primary vaginal pathogen and assesses the efficacy and safety of therapy with a single, 2-g oral dose of metronidazole. over a period of 20 months, 882 symptomatic adolescent girls had positive cultures for g. vaginalis. all were post-menarcheal. no other vaginal pathogen was isolated in 609. the total group had a second culture 5-7 days after treatment. cultures taken after treatment were negative in 95% of those treated with a single 2-g do ...19836345494
gardnerella vaginalis-associated vaginitis--a 'new' sexually transmitted disease. 19836345982
[sensitivity of gardnerella vaginalis to antibiotics. study of 40 strains isolated at the purpan university hospital center of toulouse].40 strains of gardnerella vaginalis were tested for their in vitro susceptibility to 10 antibiotics, by an agar dilution method. all strains were inhibited by 0,5 microgram or less of penicillin and ampicillin. erythromycin was the most active of the antibiotics tested; for all strains the minimal inhibitory concentrations were less than or equal to 0.06 microgram/ml. all strains were inhibited by 8 micrograms of streptomycin per ml and 4 micrograms of chloramphenicol per ml. 50% of the strains ...19836348664
a placebo-controlled, double-blind comparison of tinidazole and triple sulfonamide cream for the treatment of nonspecific vaginitis.oral tinidazole, 500 mg twice daily for 5 days, was compared to triple sulfonamide cream inserted into the vagina twice daily for 7 days for the treatment of nonspecific vaginitis in a placebo-controlled, double-blind trial. at 1 and 3 weeks after treatment, 29 of 30 and 18 of 19 patients, respectively, who had been given tinidazole were cured, as compared to 16 of 27 and 6 of 14 patients, respectively, who had been given oral placebo together with a vaginal cream with or without sulfonamides (p ...19836351619
anaerobic bacteria as cause of infections in female genital organs.anaerobic bacteria constitute a substantial component of the normal vaginal flora and of the outer cervical canal. consequently, one would expect infections emanating from the vaginal flora to be caused to a substantial degree by anaerobic bacteria. the anaerobes may contribute in colpitis, but their role is difficult to prove in this situation, since sampling only yields normal flora components. one clue that anaerobes may contribute to colpitis is the circumstance that the flora under those co ...19836353553
nonspecific vaginitis in an outpatient clinic. comparison of three dosage regimens of metronidazole.nonspecific vaginitis causes diagnostic as well as therapeutic difficulties in gynecological private practice. in a study in 63 patients with "nonspecific" vaginitis, clinical parameters were related to the bacteriological isolation of g. vaginalis in order to try to find criteria which can help to make a correct diagnosis of "nonspecific vaginitis". these patients were treated with three different regimens of metronidazole, either 2000 mg as a single dose, or two single doses of 2000 mg each ta ...19836364329
a dose-duration study of metronidazole for the treatment of nonspecific vaginosis. 19836364332
treatment of non-specific vaginitis with a single dose of tinidazole.in a double-blind, randomized trial twenty-six patients with non-specific vaginitis were treated with tinidazole/placebo (4 x 500 mg in one single dose). thirteen patients received placebo, of which one was cured. thirteen patients received tinidazole, of which six were cured. the nineteen non-responders received tinidazole (4 x 500 mg in one single dose), which resulted in the cure of eight patients (seven from the original placebo-group and one from the original tinidazole-group). of the eleve ...19836364333
comparison of two different regimens of metronidazole in the treatment of non-specific vaginitis.25 patients with symptoms of non-specific vaginitis have been treated with metronidazole either 2000 mg in a single dose or 400 mg three times daily for 5 days. diagnosis was settled by 1) identification of g. vaginalis in culture of vaginal secretion, 2) the observation of clue cells in wet smear, 3) a positive amine test on application of potassium hydroxide, and 4) increased ph in the vaginal secretion. most patients' symptoms and complaints disappeared and at the same time most of the tests ...19836364334
sexually transmitted vaginitis.urologic complaints in both men and women may be related to sexually transmissible infectious agents that frequently cause vaginitis in women. the author reviews the etiology, epidemiology, diagnosis, treatment, and clinical presentations of the various types of vaginitis. vaginal infections in women often have their counterparts in men. control of these communicable infections requires a better understanding of the vaginitis syndromes.19846369701
biology of sexually transmitted diseases.the clinical concept of sexually transmitted diseases describes a common mode of transmission of pathogens spanning the full spectrum of medical microbiology. these organisms have few common biologic characteristics aside from their ability to colonize or invade particular anatomic sites. new information on the biology of sexually transmitted pathogens has led to improved diagnostic and therapeutic strategies. understanding that patients with sexually transmitted pathogens may be asymptomatic or ...19846369702
[role of gardnerella vaginalis and anaerobes. etiology of nonspecific colpitis].nonspecific vaginitis is a very common clinical syndrome. in patients with nonspecific vaginitis the normal, lactobacillus-dominated microbial flora is replaced by gardnerella vaginalis and certain anaerobes, preferably bacteroides spp. the mechanisms by which anaerobes or gardnerella vaginalis or a combination of these organisms produce the disease condition are still unknown. treatment with ampicillin and certain other drugs is often effective, but metronidazole appears to be the most effectiv ...19846370810
[diagnosis, clinical aspects and therapy of vulvovaginitis in daily medical practice]. 19846371989
isolation of gardnerella vaginalis from routine genito-urinary tract specimens.routine screening of 2374 high vaginal swabs yielded 132 cultures (5.6 per cent) which were positive for gardnerella vaginalis. thirty-four of the strains were isolated together with candida albicans and 46 with trichomonas vaginalis. one hundred and twenty (90.9 per cent) of the isolates were from females between the ages of 16 and 40 years. one hundred and nineteen (90 per cent) patients sought medical advice because of a history of discharge (88) or inflammation (31). the other 13 (10 per cen ...19846373950
gardnerella vaginalis vaginitis. the current opinion.gardnerella vaginalis, a gram-negative bacillus, was first described in 1954. the associated clinical entity, vaginitis, first described in 1955, is controversial. the disease has specific symptoms, signs and diagnostic criteria. it has a symbiotic relationship with anaerobic bacteria. whether g. vaginalis is a pathogen or normal inhabitant of the vagina is a subject of debate. the term nonspecific vaginitis should not be used for g. vaginalis vaginitis. g. vaginalis is sexually transmitted.19846374137
metronidazole in the treatment of non-specific vaginitis (nsv).in a large multicentre study of 429 patients with the usual signs and symptoms of non-specific vaginitis (nsv), we studied the effect of different doses of metronidazole. the patients were divided into five treatment groups as follows: group a was given 400 mg metronidazole three times daily for seven days, group b 2000 mg as a single dose, group c 2000 mg on days 1 and 2, group d 2000 mg on days 1 and 3, and group e was given 1200 mg metronidazole once daily for five days. at follow up examinat ...19846375804
an evaluation of single-dose metronidazole treatment for gardnerella vaginalis vaginitis.a randomized, nonblinded study was carried out to evaluate the efficacy of a single 2-g oral dose of metronidazole (flagyl) versus a seven-day regimen in the treatment of "nonspecific" vaginitis. using cultures for gardnerella vaginalis, 14 (67%) of 21 women treated with the single 2-g dose and 19 (86%) of 21 women receiving the seven-day course were considered cured seven to ten days after treatment (p greater than .10). no difference in compliance was noted between the two groups, and the inci ...19846377153
[adherence of staphylococcus epidermidis, escherichia coli and corynebacterium vaginale to vaginal epithelial cells of healthy women and patients with chronic inflammation of the adnexa]. 19846380769
recovery of gardnerella vaginalis from blood by the quantitative direct plating method.this report describes a case of gardnerella vaginalis sepsis in an obstetric patient whose blood cultures were negative by the conventional bactec system but positive by the quantitative direct plating method, which involves the direct inoculation of blood samples on chocolate agar and blood agar plates.19846386869
[gardnerella vaginalis infection--another sexually transmitted disease].the gardnerella vaginalis infection of the urogenital tract, an std, is of clinical importance in females and of epidemiological importance in males. females suffer from vulvovaginitis amine colpitis, with a bad-smelling grey vaginal discharge with a ph of 5.0-5.5, which contains "clue cells". the urethra of males is often asymptomatically infected. the identification of g. vaginalis is time-consuming and requires a lot of material. isolation and identification of g. vaginalis can not yet be mad ...19846389437
the role of gardnerella vaginalis in 'non-specific' vaginitis. 19846389712
the prevalence, six-month persistence, and predictive values of laboratory indicators of bacterial vaginosis (nonspecific vaginitis) in asymptomatic women.the natural course of signs and laboratory test findings indicative of bacterial vaginosis was followed in an observational noninterventive 6-month longitudinal study of 270 asymptomatic women. only the minority of positive gardnerella vaginalis cultures (5 of 33), wet mount clue cells (5 of 14), sniff tests (3 of 11), papanicolaou smear clue cells (0 of 5), and discharge consistent with bacterial vaginosis (11 of 49) persisted in the absence of therapy. while these four laboratory parameters as ...19846391177
infections of the genitourinary tract in women: selected aspects. 19846397045
anaerobic curved rods in genital samples of women. performance of different selective media, comparison of detection by microscopy and culture studies, and recovery from different sampling sites.anaerobic curved rods (cr) frequently occur in the vaginal flora of women with non-specific vaginitis, more recently referred to as bacterial vaginosis (bv). the reasons for difficulties in culturing cr include their anaerobic nature, slow rate of growth and presence in a highly mixed flora. the present study concerns the efficiency of three culture media--blood agar, a gonococcal medium, and columbia agar--for recovery of cr. the possibility of improving selectivity by adding various antibiotic ...19846399403
bacteriology of the vagina.interest in the microflora of the vagina and cervix has stemmed from the recognition of its significant association with vaginal and upper genital tract infections. yet a clear understanding of the range of microbial types which are consistent with a normal genital tract and the factors which control the flora are elusive. different methods and study populations in the various studies of the vaginal flora often have produced divergent conclusions. studies using improved bacteriologic culture tec ...19846399406
the bacteriology of gardnerella vaginalis.even 70 years ago gram-negative coccobacilli had been recognized in vaginal discharge and were cultured 30 years ago. the need to have blood in agar medium for cultivation suggested that the organisms might be a haemophilus species. later, however, growth characteristics and other features resulted in their being placed in the genus corynebacterium, before it was realized that this was inappropriate and they were transferred to a new genus and species gardnerella vaginalis. the organisms are gra ...19846399409
microbiology of vaginal discharge in nairobi, kenya.among women attending a sexually transmitted disease (std) clinic in nairobi with vaginal discharge, neisseria gonorrhoeae and chlamydia trachomatis were isolated from the cervix in 32 (26%) of 122 and four (7%) of 58 women respectively. infection with trichomonas vaginalis, candida albicans, gardnerella vaginalis, and mycoplasma spp were diagnosed in 42 of 122 (34%), 26 of 110 (24%), 75 of 100 (75%), and 42 of 89 (47%) women respectively. mixed infections with at least two pathogens were found ...19836405973
abc of sexually transmitted diseases. vaginal discharge: diagnosis. 19836416487
vaginal discharge. 19846418324
measurement of microbial alpha-amylases with p-nitrophenyl glycosides as the substrate complex.the detection of alpha-amylase is commonly used in clinical microbiology laboratories to aid in differentiating streptococcus bovis from other streptococci. it is also useful in identifying eikenella corrodens and the gravis subspecies of corynebacterium diphtheriae and in separating species of the genera bacteroides, clostridium, actinomyces, and bacillus. currently, the most frequently used procedure utilizes starch as the substrate and iodine as the indicator. starch is incorporated into a ag ...19846418764
isolation and identification of gardnerella vaginalis. 19836420883
activity of ciprofloxacin against genital tract pathogens.the in vitro activity of the quinolone carboxylic acid, ciprofloxacin, against a variety of genital tract pathogens was examined. each of 35 isolates of neisseria gonorrhoeae, including some beta-lactamase producing strains and strains resistant to tetracycline, was inhibited at a concentration of 0.01 mg/l. most (13 of 20) strains of gardnerella vaginalis were inhibited at 1 mg/l but three isolates had minimum inhibitory concentrations (mics) of 8 mg/l or more. each of seven strains of chlamydi ...19846435814
motile curved rods in women attending a std clinic.motile anaerobic curved rods were cultured from vaginal discharge of 19 of 262 women attending an std clinic. in 26 women, motile rods were observed by examination of wet smears. in all 5 specimens culture-positive for motile anaerobic curved rods of the long type, motile rods were observed in the wet smears, while in only 6 of the 14 specimens culture-positive for motile anaerobic curved rods of the short type, were motile rods seen in wet smears.19846443163
recovery of anaerobic curved rods and gardnerella vaginalis from the urethra of men, including male heterosexual consorts of female carriers.three hundred and nine men attending a clinic for sexually transmitted diseases were studied regarding urethral colonization with anaerobic curved rods (cr), gardnerella vaginalis, bacteroides ureolyticus, as well as for chlamydiae and gonococci. ten (3.2%) of the men harboured cr. all strains of cr were of the short variant (approximately 1 micron long). fourteen (4.5%) men were culture-positive for g. vaginalis and 58 (18.7%) for b. ureolyticus. of the four men who were culture-positive for cr ...19846443164
quantitative bacteriology of the vaginal flora in genital disease.samples from the posterior vaginal fornix of 102 women with various clinical conditions were analysed by a quantitative method. aerobes were isolated from all but one of the specimens at a mean concentration of 7.2 log10 cfu/g and anaerobes from 92 specimens at a mean concentration of 8.1 log10 cfu/g. in most clinical conditions and in a control group of asymptomatic women, anaerobes outnumbered aerobes by about ten to one (one log10 unit). the most common organisms were aerobic and anaerobic la ...19846492119
sexually transmittable diseases and other genital infections during adolescence.sexually transmitted (std), diseases in which sexual contact is epidemiologically important but not the only mode of acquisition, will continueto increase in incidence unless effective control strategies can be applied. the recent increase in incidence has been due, in part, to increased levels of sexual activity among young people. focus in this discussion is on the epidemiology of std, the importance of an accurate sexual history in the diagnosis of std, gonorrhea, syphilis, nongonococcal ur ...19846547744
unsuspected chlamydia trachomatis in females attending a sexually transmitted diseases clinic.one hundred and five women attending auckland sexually transmitted diseases clinic were reviewed because of positive cervical cultures for chlamydia trachomatis. their average age was 19 years, 70% were european; 28% were maori. in half the patients the positive culture was a chance finding and therefore treatment was delayed resulting in six patients (12%) developing pelvic inflammatory disease. eight out of nine male contacts, subsequently traced, had asymptomatic urethritis, one having epidid ...19836577348
diagnosis and therapy of nonspecific vaginitis. correlation between koh-test, clue cells and microbiology.a clinical and quantitative microbiological study was performed on 32 women with fishy odour of the vaginal fluid. this odour could be intensified by the addition of one drop of 10% potassium hydroxide. clue cells were found to be present in all cases. gardnerella vaginalis was isolated from the vaginal fluid in 97%, and large quantities of mixed anaerobes in 100% of the patients. the therapy with metronidazole, 400 mg twice daily for 5 days, was successful in 22 out of 24 patients. after therap ...19836582591
nonspecific vaginitis. diagnostic criteria and microbial and epidemiologic associations.numerous previous studies of nonspecific vaginitis have yielded contradictory results regarding its cause and clinical manifestations, due to a lack of uniform case definition and laboratory methods. we studied 397 consecutive unselected female university students and applied sets of well defined criteria to distinguish nonspecific vaginitis from other forms of vaginitis and from normal findings. using such criteria, we diagnosed nonspecific vaginitis in up to 25 percent of our study population; ...19836600371
the identification of gardnerella vaginalis.a collection of 72 strains of catalase-negative gram-positive, -negative and -variable cocco-bacilli isolated from samples of vaginal discharge from women with non-specific vaginal infection was examined in an attempt to develop an identification system for gardnerella vaginalis that could be used in a diagnostic laboratory. carbohydrate fermentation tests were found to be poorly reproducible and of little differentiating value. enzyme tests were found similarly unhelpful, as were many antibioti ...19836600500
gardnerella vaginalis and nonspecific vaginitis. 19836600636
problem-orientated categorisation of "other conditions" seen in a genitourinary medicine clinic.the case sheets of patients coded as d2 (other conditions requiring treatment) and d3 (other conditions not requiring treatment) in 1981 were reclassified on a problem-orientated basis. ten discreet categories were delineated with ease. women with non-specific vaginitis and men with gardnerella vaginalis urogenital infection, usually coded d2 or c4, should be reclassified under the c group of conditions as, for example, "gardnerella or other bacterial genital infections." it is suggested that co ...19836600641
metronidazole.metronidazole, a nitroimidazole derivative, is a unique antimicrobial agent that is active against both bacterial and parasitic organisms, although only the anaerobic members of these groups are susceptible. it has been used for the treatment of trichomoniasis for about 20 years and is also effective against amebiasis and giardiasis. more recently, metronidazole has emerged as a principal agent for the treatment of anaerobic bacterial infections. it is highly effective against all species of ana ...19836600804
gardnerella vaginalis and anaerobic bacteria in genital disease.in a study of gardnerella vaginalis and anaerobic bacteria in non-specific vaginitis (nsv) and other genital disease 89 patients attending a genital medicine clinic had vaginal samples examined for conventional pathogens and for quantitative analysis of g vaginalis and aerobic and anaerobic bacterial flora. the overall incidence of g vaginalis was 20%; g vaginalis (mean concentration 7.0 log10/g of secretion) occurred predominantly in patients with nsv (57%) but also in sexual contacts of non-sp ...19836600955
minimal criteria for the identification of gardnerella vaginalis isolated from the vagina.vaginal swabs were examined for the presence of gardnerella vaginalis. of 294 isolates with appropriate colonial and cellular morphology subjected to an identification procedure, 203 (69%) were identified as g vaginalis. the 91 isolates not identified as g vaginalis were differentiated by their inability to ferment starch, cause diffuse beta haemolysis on human blood agar or hydrolyse hippurate. other tests, often used in the identification of g vaginalis, were found to be insufficiently specifi ...19836601114
vaginal disease. venereal and nonvenereal types.vaginal diseases are, in many cases, sexually transmitted. however, most can also be non-venereal and can occur in any age-group. special care should be taken in examining and treating young females with vaginal disease, and the possibility of sexual abuse should be considered in this age-group. finally, special attention should be paid to girls and women exposed to diethylstilbestrol in utero, as these patients have an increased risk of anatomic and reproductive anomalies.19836601795
isolation and identification of anaerobic organisms from the male and female urogenital tracts.since bacteroides spp may play an important role together with gardnerella vaginalis in the pathogenesis of non-specific vaginitis and balanoposthitis, anaerobic organisms were isolated and identified from 34 female and 20 male patients attending a department of genitourinary medicine, and the results compared with the clinical findings and the presence of other routinely isolated pathogens. twenty-three different organisms were recovered from 13 of the 20 men; of these organisms, 20 were of the ...19836601970
unconventional bacteria in urinary tract disease: gardnerella vaginalis.bladder aspirate urine samples (n = 190) were cultured for the presence of fastidious microorganisms. these samples were obtained from patients with urinary tract disease in whom standard bacteriologic investigation had failed to indicate infection. gardnerella vaginalis was recovered alone, or in association with ureaplasma urealyticum from the bladder urine of 33% of patients with reflux scarring. g. vaginalis was localized to the upper urinary tract in 75% of these patients with bladder count ...19836604191
amine content of vaginal fluid from patients with trichomoniasis and gardnerella associated non-specific vaginitis.amounts of putrescine, cadaverine, and tyramine were measured in vaginal washings from five patients with non-specific vaginitis (nsv) associated with gardnerella vaginalis, five patients with trichomoniasis, and five healthy controls. putrescine and cadaverine were present in all but one sample from the infected patients; tyramine was found in four of five samples from the women with nsv and in two of five samples from those with trichomoniasis. the mean cadaverine to putrescine ratio was signi ...19836604557
semiquantitative culture of gardnerella vaginalis in laboratory determination of nonspecific vaginitis.to evaluate the usefulness of quantitative cultures of gardnerella vaginalis in the laboratory determination of nonspecific vaginitis, the actual and relative numbers of g. vaginalis in genital cultures of a general patient population were assessed semiquantitatively, and the laboratory results were then correlated with the clinical findings. of the 1,585 women studied, 417 (26.3%) yielded g. vaginalis in culture. of these, only 113 (27.1%) were found to have symptoms and signs consistent with n ...19836604735
bacteriuria due to ureaplasma urealyticum and gardnerella vaginalis in women with preeclampsia.certain fastidious organisms such as u urealyticum and g vaginalis can be isolated from the aspirated bladder urine of pregnant women more frequently than conventional urinary pathogens such as escherichia coli [1]. they can be isolated even more often from the aspirated bladder urine of patients with renal disease, but rarely from that of healthy men or nonpregnant women [2]. we investigated the incidence of bacteriuria due to these two organisms--particularly u urealyticum--in patients with pr ...19836604763
vaginitis. 19836605671
susceptibility of gardnerella vaginalis to cephradine.the activity of cephradine and the influence of ph on its activity against 70 gardnerella vaginalis strains were determined. serial dilutions of cephradine (0.062 to 256 micrograms/ml) were incorporated into dunkelberg agar, inoculated with a steers replicator, incubated in 5% co2 for 48 h, and examined. the minimal inhibitory concentrations for 90% of the isolates were 16 and 8.0 micrograms/ml at ph 7.0 to 6.0 and 5.5, respectively.19836605720
non-volatile fatty acids in the diagnosis of non-specific vaginitis.in the vaginal washings of 100 women with symptomatic non-specific vaginitis a succinate/lactate ratio of greater than or equal to 0.4 had a diagnostic sensitivity of 80%, a specificity of 83% for this condition. the predictive value of a positive test was 94%, but that of a negative test was only 55%. a strong association between the presence of gardnerella vaginalis, anaerobes, a vaginal ph of above 4.5, and amines was found not only in non-specific vaginitis, but also in trichomonal and gonoc ...19836606653
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