| is the grass greener on the other side? encouraging the development of synthetic biology in europe. | | 2008 | 18762774 |
| [mycoplasma genitalium detection and correlation with clinical manifestations in population of the zulia state, venezuela]. | diverse studies demonstrate an association between mycoplasma genitalium and urogenital pathologies. the aim of this study was to investigate the prevalence of m. genitalium in patients attending gynecological evaluation in private clinics (n = 172). dna amplification assays of the genes 16s rrna and mgpa were utilized. the prevalence of m. genitalium in the study population was 7.5%. m. genitalium was detected in 12.1% and 4.1% of the symptomatic and asymptomatic patients, respectively (p = 0.0 ... | 2008 | 18769771 |
| azithromycin and moxifloxacin for microbiological cure of mycoplasma genitalium infection: an open study. | there are no evidence-based guidelines for the treatment of mycoplasma genitalium-positive genital infection. in a retrospective survey, we analysed the treatment results of patients tested for m. genitalium at olafia, unit for sexual transmitted diseases in oslo. out of 10,109 patients, 452 had a positive polymerase chain reaction. between 72% and 100% of patients in the different treatment groups returned for test of cure after four to five weeks. first-line treatment with 1 g single dose azit ... | 2008 | 18824619 |
| the mycoplasma pneumoniae mpn229 gene encodes a protein that selectively binds single-stranded dna and stimulates recombinase a-mediated dna strand exchange. | mycoplasma pneumoniae has previously been characterized as a micro-organism that is genetically highly stable. in spite of this genetic stability, homologous dna recombination has been hypothesized to lie at the basis of antigenic variation of the major surface protein, p1, of m. pneumoniae. in order to identify the proteins that may be involved in homologous dna recombination in m. pneumoniae, we set out to characterize the mpn229 open reading frame (orf), which bears sequence similarity to the ... | 2008 | 18831760 |
| interaction of mycoplasma genitalium with host cells: evidence for nuclear localization. | mycoplasma genitalium (mg) is a mollicute that causes a range of human urogenital infections. a hallmark of these bacteria is their ability to establish chronic infections that can persist despite completion of appropriate antibiotic therapies and intact and functional immune systems. intimate adherence and surface colonization of mycoplasmas to host cells are important pathogenic features. however, their facultative intracellular nature is poorly understood, partly due to difficulties in develo ... | 2008 | 18832309 |
| role of mycoplasma genitalium mg218 and mg317 cytoskeletal proteins in terminal organelle organization, gliding motility and cytadherence. | the terminal organelle is a differentiated structure that plays a key role in mycoplasma cytadherence and locomotion. for this reason, the analysis of mycoplasma genitalium mutants displaying anomalous terminal organelles could improve our knowledge regarding the structural elements required for proper locomotion. in this study, we isolated several m. genitalium mutants having transposon insertions within the mg218 or mg317 genes, which encode the orthologues of mycoplasma pneumoniae hmw2 and hm ... | 2008 | 18832324 |
| mycoplasma genitalium in women with lower genital tract inflammation. | to examine the prevalence of mycoplasma genitalium in a large number of female patients attending a sexually transmitted infections (sti) clinic and to determine if there is an association with signs or symptoms of lower genital tract inflammation (lgti). | 2009 | 18842689 |
| mycoplasma genitalium is associated with symptomatic and asymptomatic non-gonococcal urethritis in men. | to examine the prevalence of mycoplasma genitalium in a large number of male patients attending a sexually transmitted infections (sti) clinic and to determine if there is an association with objective non-gonococcal urethritis (ngu) in patients with and without clinical symptoms. | 2009 | 18842690 |
| [diagnosis of mycoplasma genitalium by mgpa and rrna 16s gene amplification]. | mycoplasma genitalium has been associated with nongonococcal urethritis (ngu). diagnosis by pcr has become the primary detection method for this organism. thus, diagnosis by dna amplification using the pcr technique should be utilized. | 2008 | 18852931 |
| prevalence of mycoplasma genitalium in health clinic attendees complaining of vaginal discharge in bangladesh. | the objective of the study was to determine the prevalence of mycoplasma genitalium in a sample of health clinic attendees complaining of vaginal discharge. a subsample of 399 vaginal and cervical swabs was randomly selected from 2579 samples collected during a study to determine the causes of vaginal discharge in women attending primary health-care clinics in dhaka, bangladesh. cervical samples were tested for m. genitalium by polymerase chain reaction. in addition, the samples were tested for ... | 2008 | 18931272 |
| persistence of mycoplasma genitalium following azithromycin therapy. | to determine clinical outcomes and cure rates for m.genitalium genital infection in men and women following azithromycin 1 g. | 2008 | 18978939 |
| azithromycin treatment failure in mycoplasma genitalium-positive patients with nongonococcal urethritis is associated with induced macrolide resistance. | mycoplasma genitalium is a common cause of nongonococcal urethritis. treatment trials have shown that doxycycline is inefficient, whereas a 5-day course of azithromycin eradicates the bacterium from 95% of infected men. the aim of the study was to establish the reason for the occasional treatment failures. | 2008 | 18990060 |
| nongonococcal urethritis and antibiotic-resistant mycoplasma genitalium infection. | | 2008 | 18990063 |
| clinical presentation of mycoplasma genitalium infection versus neisseria gonorrhoeae infection among women with pelvic inflammatory disease. | women with pelvic inflammatory disease (pid) often present with a spectrum of symptoms. the characteristics of nongonococcal, nonchlamydial pid have not been well described. our objective was to examine the characteristics of mycoplasma genitalium infection among women with clinically suspected pid. | 2009 | 19025498 |
| the gene mpn310 (hmw2) from mycoplasma pneumoniae encodes two proteins, hmw2 and hmw2-s, which differ in size but use the same reading frame. | the gene mpn310 from mycoplasma pneumoniae encodes the proteins hmw2 with a molecular weight of 215 621 and the smaller p28, here called hmw2-s. because hmw2-s is not well defined, it was isolated from protein extracts of m. pneumoniae cells and its n-terminal end was determined by ms. hmw2-s starts with the methionine at the amino acid position 1620 of hmw2 and its residual sequence is identical to the last 198 amino acids of hmw2, predicting a molecular weight of 23 204. these results were con ... | 2009 | 19025563 |
| monitoring of mycoplasma genitalium growth and evaluation of antibacterial activity of antibiotics tetracycline and levofloxacin using a wireless magnetoelastic sensor. | mycoplasma genitalium (mg) is the smallest and simplest self-replicating bacteria lacking of cell wall and is a human pathogen causing various diseases. this paper describes the real-time, long-term and in situ monitoring of the growth of mg and evaluation of the effect of the antibiotics tetracycline and levofloxacin on the growth using a wireless magnetoelastic sensor. the sensor is fabricated by coating a magnetoelastic strip with a polyurethane protecting film. in response to a time-varying ... | 2009 | 19041236 |
| alcohol abuse, sexual risk behaviors, and sexually transmitted infections in women in moshi urban district, northern tanzania. | to assess the covariates of alcohol abuse and the association between alcohol abuse, high-risk sexual behaviors and sexually transmitted infections (stis). | 2009 | 19060779 |
| one-step assembly in yeast of 25 overlapping dna fragments to form a complete synthetic mycoplasma genitalium genome. | we previously reported assembly and cloning of the synthetic mycoplasma genitalium jcvi-1.0 genome in the yeast saccharomyces cerevisiae by recombination of six overlapping dna fragments to produce a 592-kb circle. here we extend this approach by demonstrating assembly of the synthetic genome from 25 overlapping fragments in a single step. the use of yeast recombination greatly simplifies the assembly of large dna molecules from both synthetic and natural fragments. | 2008 | 19073939 |
| endocervical swabs transported in first void urine as combined specimens in the detection of mycoplasma genitalium by real-time pcr. | the aim of this study was to determine whether a patient's endocervical swab specimen can be transported in first void urine (fvu) as combined specimens for the detection of mycoplasma genitalium by real-time pcr. the study also compared two different dna extraction methods for observation of possible pcr inhibition. three specimens, one endocervical swab specimen transported in 2-sp medium, one endocervical swab specimen transported in fvu and a fvu specimen, were collected from 329 women. all ... | 2009 | 19074662 |
| [mycoplasma genitalium in men with non-gonococcal urethritis]. | c. trachomatis is the underlying cause of 20?-?50 % of sexually transmitted urethritis cases. data from the last 10?-?15 years indicate that m. genitalium may be a cause, but the prevalence of m. genitalium in norwegian patients has not previously been assessed or published. | 2008 | 19079416 |
| buffering by gene duplicates: an analysis of molecular correlates and evolutionary conservation. | one mechanism to account for robustness against gene knockouts or knockdowns is through buffering by gene duplicates, but the extent and general correlates of this process in organisms is still a matter of debate. to reveal general trends of this process, we provide a comprehensive comparison of gene essentiality, duplication and buffering by duplicates across seven bacteria (mycoplasma genitalium, bacillus subtilis, helicobacter pylori, haemophilus influenzae, mycobacterium tuberculosis, pseudo ... | 2008 | 19087332 |
| clinical efficacy of azithromycin for male nongonococcal urethritis. | the aim of this study was to confirm the clinical efficacy of a single-dose azithromycin (azm) regimen (1000 mg) for patients with nongonococcal urethritis in real-life practice. the study finally evaluated 55 patients, 42 who were symptomatic and 13 who were asymptomatic, after excluding 40 who visited clinics only once. sixteen of the symptomatic patients were diagnosed as having nongonococcal chlamydial urethritis, 7 as having nongonococcal nonchlamydial urethritis, and 19 as having urethriti ... | 2008 | 19089553 |
| mycoplasma genitalium-encoded mg309 activates nf-kappab via toll-like receptors 2 and 6 to elicit proinflammatory cytokine secretion from human genital epithelial cells. | mycoplasma genitalium has been implicated in several important reproductive tract syndromes in women, including pelvic inflammatory disease, cervicitis, and tubal factor infertility. the mechanisms of immune activation are unclear, and we sought to determine whether m. genitalium was capable of activating innate immune responses through ligation of highly expressed toll-like receptors (tlr) of the genital tract. using hek293 cells expressing specific human tlr, viable m. genitalium and the recom ... | 2009 | 19103762 |
| [non-chlamydial non-gonococcal urethritis]. | besides chlamydia trachomatis, various microorganisms could cause non-gonococcal urethritis (ngu). recently, mycoplasma genitalium and ureaplasma urealyticum (biovar 2) have been suggested to be other pathogens of ngu independent of c. trachomatis. clinical findings of non-chlamydial ngu, including m. genitalium--or u. urealyticum-postive ngu, are not different from those of chlamydial ngu. m. genitalium and u. urealyticum (biovar 2) are susceptible to tetracyclines, macrolides, and fluoroquinol ... | 2009 | 19177768 |
| association of mycoplasma genitalium and hiv infection: a systematic review and meta-analysis. | to systematically review studies of mycoplasma genitalium and the association with hiv infection in adults and to summarize the findings in a meta-analysis. | 2009 | 19194271 |
| [utility of molecular biology techniques in the diagnosis of sexually transmitted diseases and genital infections]. | historically, the diagnosis of sexually transmitted diseases (stds) has been difficult. the introduction of molecular biology techniques in microbiological diagnosis and their application to non-invasive samples has produced significant advances in the diagnosis of these diseases. overall, detection of neisseria gonorrhoeae by molecular biology techniques provides a presumptive diagnosis and requires confirmation by culture in areas with a low prevalence. for chlamydia trachomatis infections, th ... | 2008 | 19195446 |
| a genome-scale metabolic reconstruction of mycoplasma genitalium, ips189. | with a genome size of approximately 580 kb and approximately 480 protein coding regions, mycoplasma genitalium is one of the smallest known self-replicating organisms and, additionally, has extremely fastidious nutrient requirements. the reduced genomic content of m. genitalium has led researchers to suggest that the molecular assembly contained in this organism may be a close approximation to the minimal set of genes required for bacterial growth. here, we introduce a systematic approach for th ... | 2009 | 19214212 |
| population-based assessment of mycoplasma genitalium in vietnam--low prevalence among married women of reproductive age in a rural area. | to analyse the prevalence of mycoplasma genitalium infection in a population-based study among married women from a demographic surveillance site in a rural geographical area of vietnam. | 2009 | 19220645 |
| azithromycin treatment for nongonococcal urethritis negative for chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum. | some patients with nongonococcal urethritis (ngu) are negative for chlamydia trachomatis, mycoplasmas, and ureaplasmas. the optimal antimicrobial chemotherapy for such ngu has not fully been clarified. we assessed the efficacy of azithromycin for treatment of nonmycoplasmal, nonureaplasmal, nonchlamydial ngu (nmnuncngu). thirty-eight men whose first-pass urine was negative for chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum were tre ... | 2009 | 19228227 |
| mycoplasma genitalium in men who have sex with men at male-only saunas. | to determine the prevalence and clinical associations of mycoplasma genitalium in urethral, rectal and pharyngeal specimens collected from men who have sex with men (msm) attending male-only saunas. | 2009 | 19254902 |
| comparative in vitro susceptibilities of human mycoplasmas and ureaplasmas to a new investigational ketolide, cem-101. | mics were determined for an investigational ketolide, cem-101, and azithromycin, telithromycin, doxycycline, levofloxacin, clindamycin, and linezolid against 36 mycoplasma pneumoniae, 5 mycoplasma genitalium, 13 mycoplasma hominis, 15 mycoplasma fermentans, and 20 ureaplasma isolates. all isolates, including two macrolide-resistant m. pneumoniae isolates, were inhibited by cem-101 at < or = 0.5 microg/ml, making cem-101 the most potent compound tested. | 2009 | 19258276 |
| multiplex pcr testing detection of higher-than-expected rates of cervical mycoplasma, ureaplasma, and trichomonas and viral agent infections in sexually active australian women. | knowing the prevalence of potential etiologic agents of nongonococcal and nonchlamydial cervicitis is important for improving the efficacy of empirical treatments for this commonly encountered condition. we describe four multiplex pcrs (mpcrs), designated vdl05, vdl06, vdl07, and vdl09, which facilitate the detection of a wide range of agents either known to be or putatively associated with cervicitis, including cytomegalovirus (cmv), enterovirus (ev), epstein-barr virus (ebv), varicella-zoster ... | 2009 | 19261782 |
| analysis identifying common and distinct sequences among texas clinical strains of mycoplasma genitalium. | mycoplasma genitalium is a human bacterial pathogen linked to urethritis and other sexually transmitted diseases. here, we assessed the incidence of m. genitalium infection in patients attending a sexually transmitted disease clinic in san antonio, tx, by use of diagnostic real-time pcr. overall, 16.8% of women and 15.1% of men were found m. genitalium positive. regions of the mgpb gene, which encodes the mgpa adhesin, were amplified from positive clinical specimens and evaluated for sequence va ... | 2009 | 19261784 |
| anal intercourse among young heterosexuals in three sexually transmitted disease clinics in the united states. | to examine factors associated with heterosexual anal intercourse (ai). | 2009 | 19265740 |
| prevalence of urogenital mycoplasmas and ureaplasmas in women after kidney transplantation. | the prevalence of urogenital mycoplasmas and ureaplasmas in kidney transplant and hemodialyzed patients was studied. | 2009 | 19300187 |
| association of mycoplasma genitalium with acute non-gonococcal urethritis in russian men: a comparison with gonococcal and chlamydial urethritis. | urethral specimens from 172 men who attended sexually transmitted disease clinics in the moscow oblast were examined for neisseria gonorrhoeae, chlamydia trachomatis and mycoplasma genitalium by nucleic acid amplification tests. n. gonorrhoeae was detected in the urethra of 41 (24%) of the 172 men and c. trachomatis in 57 (33%). the latter occurred in 15 (36%) of the 41 men who were infected by n. gonorrhoeae and in 42 (32%) of 131 uninfected by gonococci. of the 42 men uninfected by gonococci b ... | 2009 | 19304966 |
| mycoplasma genitalium: a significant urogenital pathogen ? | detection of mycoplasma infections, especially those caused by mycoplasma genitalium (m. genitalium) is not a part of routine microbiological diagnostics, as commercial strip tests based on microculture in test wells do not include m. genitalium in their identification spectrum. also classical methods of culture are especially demanding in the case of m. genitalium strains. pcr technique with application of specific starter oligonucleotides is currently used in the diagnostics of m. genitalium. ... | 2009 | 19333215 |
| treatment strategies for pelvic inflammatory disease. | pelvic inflammatory disease (pid) is one of the most common infections seen in nonpregnant reproductive-age women. it is a major public health problem associated with substantial medical complications (e.g., infertility, ectopic pregnancy, and chronic pelvic pain) and healthcare costs. prevention of these long-term sequelae requires treatment strategies that are based on the microbiologic etiology of acute pid. objective: to determine appropriate antimicrobial regimens for the treatment of acute ... | 2009 | 19351231 |
| enzymatic assembly of dna molecules up to several hundred kilobases. | we describe an isothermal, single-reaction method for assembling multiple overlapping dna molecules by the concerted action of a 5' exonuclease, a dna polymerase and a dna ligase. first we recessed dna fragments, yielding single-stranded dna overhangs that specifically annealed, and then covalently joined them. this assembly method can be used to seamlessly construct synthetic and natural genes, genetic pathways and entire genomes, and could be a useful molecular engineering tool. | 2009 | 19363495 |
| mycoplasma genitalium compared to chlamydia, gonorrhoea and trichomonas as an aetiological agent of urethritis in men attending std clinics. | to investigate prevalence of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae and trichomonas vaginalis in men, frequency of co-infections, and association of organisms with urethritis in men. | 2009 | 19383597 |
| high rates of std and sexual risk behaviors among garífunas in honduras. | honduras has the highest concentration of hiv and aids cases in central america, with an estimated adult hiv prevalence of 1.5%. prevalence is higher among certain ethnic groups such as the garífuna with a reported hiv prevalence of 8%. | 2009 | 19384098 |
| a novel heuristic for local multiple alignment of interspersed dna repeats. | pairwise local sequence alignment methods have been the prevailing technique to identify homologous nucleotides between related species. however, existing methods that identify and align all homologous nucleotides in one or more genomes have suffered from poor scalability and limited accuracy. we propose a novel method that couples a gapped extension heuristic with an efficient filtration method for identifying interspersed repeats in genome sequences. during gapped extension, we use the muscle ... | 2009 | 19407343 |
| use of a dual priming oligonucleotide system to detect multiple sexually transmitted pathogens in clinical specimens. | to evaluate a new dual priming oligonucleotide (dpo)-based multiplex polymerase chain reaction (pcr) assay for detection of six sexually transmitted pathogens, including chlamydia trachomatis, neisseria gonorrhoeae, mycoplasma genitalium, mycoplasma hominis, ureaplasma urealyticum and trichomonas vaginalis. | 2009 | 19413770 |
| a randomized comparison of azithromycin and doxycycline for the treatment of mycoplasma genitalium-positive urethritis in men. | several uncontrolled observational studies have suggested that the tetracycline class of antibiotics may not be effective in treating mycoplasma genitalium infection. the present study compared the efficacy of 1 g of azithromycin given as a single dose with that of 100 mg of doxycycline given twice a day for 7 days in eliminating m. genitalium infection. | 2009 | 19438399 |
| single-dose azithromycin treatment for mycoplasma genitalium-positive urethritis: best but not good enough. | | 2009 | 19438400 |
| first evaluation of polymerase chain reaction assays used for diagnosis of mycoplasma genitalium in russia. | diagnosis of mycoplasma genitalium is entirely based on nucleic acid amplification tests (naats). in russia, several m. genitalium polymerase chain reaction (pcr) assays have been developed; however, any evaluation of their performance has never been performed. | 2009 | 19453801 |
| levofloxacin plus metronidazole in uncomplicated pelvic inflammatory disease: a preliminary study. | uncomplicated pelvic inflammatory disease (pid) is a common disease caused by numerous pathogens: sexually transmitted infections (such as chlamydia trachomatis, neisseria gonorrhoeae and mycoplasma genitalium), anaerobes, and other organisms from the vaginal flora. it is currently treated by oral route and most present protocols recommend ofloxacin plus metronidazole (met). the cdc 2006 guidelines suggested that levofloxacin (lev) can be a substitute for ofloxacin. this study aimed to evaluate ... | 2009 | 19467767 |
| association of genital mycoplasmas including mycoplasma genitalium in hiv infected men with nongonococcal urethritis attending std & hiv clinics. | acute nongonococcal urethritis (ngu) is one of the commonest sexually transmitted infections affecting men. the role of genital mycoplasmas including mycoplasma genitalium in hiv infected men with ngu is still not known. the aim of this study was to determine the isolation pattern/detection of genital mycoplasma including m. genitalium in hiv infected men with ngu and to compare it with non hiv infected individuals. | 2009 | 19491424 |
| genital mycoplasmas. | the first described pathogenic organisms that caused urethritis were neisseria gonorrhoeae and chlamydia trachomatis. the significance of detecting mycoplasma with genital swabs remained unclear for a long time. culture can differentiate between ureaplasma urealyticum and mycoplasma hominis. after introduction of nuclear acid amplification, mycoplasma genitalium was additionally detected, while gene analysis differentiates between ureaplasma urealyticum and ureaplasma parvum. mycoplasma genitali ... | 2009 | 19500195 |
| real-time pcr detection of the mg219 gene of unknown function of mycoplasma genitalium in men with and without non-gonococcal urethritis and their female partners in england. | real-time pcr was employed to detect a region of the mycoplasma genitalium mg219 gene, a gene of unknown function, in clinical samples. amplification of dna and signal production from 15 other species of human mycoplasmas and 14 other bacteria and viruses did not occur. using a panel of 208 genital and rectal samples, the sensitivity when compared to the modified mgpa gene (encoding the major surface protein mgpa) real-time pcr assay was found to be 100% and the specificity of the assay 99.5% wi ... | 2009 | 19502372 |
| 2009 european guideline on the management of male non-gonococcal urethritis. | | 2009 | 19541886 |
| amino acid changes in elongation factor tu of mycoplasma pneumoniae and mycoplasma genitalium influence fibronectin binding. | mycoplasma pneumoniae and mycoplasma genitalium are closely related organisms that cause distinct clinical manifestations and possess different tissue predilections despite their high degree of genome homology. we reported earlier that surface-localized m. pneumoniae elongation factor tu (ef-tu(mp)) mediates binding to the extracellular matrix component fibronectin (fn) through the carboxyl region of ef-tu. in this study, we demonstrate that surface-associated m. genitalium ef-tu (ef-tu(mg)), in ... | 2009 | 19546194 |
| mycoplasma genitalium in asymptomatic patients: implications for screening. | mycoplasma genitalium is well established as a cause of urethritis and has also been associated with cervicitis, endometritis and pelvic infection. low rates of infection suggest screening may be inappropriate in the general population, but it remains unclear whether asymptomatic patients attending a sexual-health clinic should be tested routinely. the objective of this study was to measure the positivity rate of m genitalium infection in asymptomatic individuals presenting to a sexual-health cl ... | 2009 | 19587392 |
| intracellular mycoplasma genitalium infection of human vaginal and cervical epithelial cells elicits distinct patterns of inflammatory cytokine secretion and provides a possible survival niche against macrophage-mediated killing. | mycoplasma genitalium is an emerging sexually transmitted pathogen that has been associated with significant reproductive tract inflammatory syndromes in women. in addition, the strong association between severity of m. genitalium infection and human immunodeficiency virus type 1 (hiv-1) shedding from the cervix suggests that innate responses to m. genitalium may influence pathogenesis of other sexually transmitted infections. epithelial cells (ecs) of the reproductive mucosa are the first cells ... | 2009 | 19602269 |
| mycoplasma genitalium: more prevalent than you think. | | 2009 | 19638896 |
| the demographic, sexual health and behavioural correlates of mycoplasma genitalium infection among women with clinically suspected pelvic inflammatory disease. | mycoplasma genitalium has been identified as a cause of pelvic inflammatory disease (pid), a clinical syndrome associated with inflammation of the female upper genital tract and serious reproductive sequelae. as the demographic, behavioural and sexual risk profile of women with m genitalium-associated pid is not well understood, the characteristics of m genitalium-infected women presenting with clinically suspected pid were investigated. | 2010 | 19703841 |
| legislation requiring monthly testing of sex workers with low rates of sexually transmitted infections restricts access to services for higher-risk individuals. | in victoria, australia, legislation requires sex workers to undergo monthly testing for gonorrhoea, chlamydia and trichomonas, and 3-monthly for hiv and syphilis, despite extremely low rates of sexually transmitted infections (sti) in female sex workers (fsw). the aim of this study was to quantify the resources and opportunities lost from this screening. | 2009 | 19703842 |
| mycoplasma genitalium as a contributor to the multiple etiologies of cervicitis in women attending sexually transmitted disease clinics. | the purpose of this study was to investigate the prevalence of chlamydia trachomatis, neisseria gonorrhoeae, trichomonas vaginalis, and mycoplasma genitalium, in women attending a sexually transmitted disease (std) clinic, as well as the frequency of coinfections, and relationship of each organism to cervicitis. | 2009 | 19704398 |
| the mycoplasma genitalium mg_454 gene product resists killing by organic hydroperoxides. | mycoplasma genitalium is the smallest self-replicating organism and a successful human pathogen associated with a range of genitourinary maladies. as a consequence of its restricted genome size, genes that are highly conserved in other bacteria are absent in m. genitalium. significantly, genes that encode antioxidants like superoxide dismutase and catalase-peroxidase are lacking. nevertheless, comparative genomics has revealed that mg_454 of m. genitalium encodes a protein with putative function ... | 2009 | 19717589 |
| persistent exposure to mycoplasma induces malignant transformation of human prostate cells. | recent epidemiologic, genetic, and molecular studies suggest infection and inflammation initiate certain cancers, including those of the prostate. the american cancer society, estimates that approximately 20% of all worldwide cancers are caused by infection. mycoplasma, a genus of bacteria that lack a cell wall, are among the few prokaryotes that can grow in close relationship with mammalian cells, often without any apparent pathology, for extended periods of time. in this study, the capacity of ... | 2009 | 19721714 |
| cloning, expression, purification, crystallization and preliminary x-ray analysis of mycoplasma genitalium protein mg289. | mycoplasma genitalium is a human pathogen that is associated with nongonococcal urethritis in men and cervicitis in women. the cloning, expression, purification and crystallization of the protein mg289 from m. genitalium strain g37 are reported here. crystals of mg289 diffracted x-rays to 2.8 a resolution. the crystals belonged to the orthorhombic space group p2(1)2(1)2(1), with unit-cell parameters a = 49.7, b = 90.9, c = 176.1 a. the diffraction data after processing had an overall r(merge) of ... | 2009 | 19724130 |
| has the time come to systematically test for mycoplasma genitalium? | | 2009 | 19734818 |
| the mycoplasma pneumoniae mpn490 and mycoplasma genitalium mg339 genes encode reca homologs that promote homologous dna strand exchange. | the p1, p40, and p90 proteins of mycoplasma pneumoniae and the mgpa and p110 proteins of mycoplasma genitalium are immunogenic adhesion proteins that display sequence variation. consequently, these proteins are thought to play eminent roles in immune evasive strategies. for each of the five proteins, a similar underlying molecular mechanism for sequence variation was hypothesized, i.e., modification of the dna sequences of their respective genes. this modification is thought to result from homol ... | 2009 | 19737894 |
| antimicrobial susceptibilities of mycoplasma genitalium strains examined by broth dilution and quantitative pcr. | only limited information regarding the antimicrobial susceptibilities of mycoplasma genitalium is available because of difficulties in isolating m. genitalium strains from clinical specimens. antimicrobial susceptibilities of 15 clinical isolates, 7 atcc strains, and an early passage of the m30 strain were examined by the broth dilution method. azithromycin, clarithromycin, sitafloxacin, and moxifloxacin were the most active drugs against m. genitalium, and their mic(90)s were 0.002, 0.008, 0.12 ... | 2009 | 19738019 |
| chlamydia trachomatis, mycoplasma genitalium, neisseria gonorrhoeae, human papillomavirus, and polyomavirus are not detectable in human tissue with epithelial ovarian cancer, borderline tumor, or benign conditions. | we sought to analyze the presence of the microorganisms chlamydia trachomatis, mycoplasma genitalium, neisseria gonorrhoeae, human papillomavirus (hpv), and the polyomaviruses bk virus (bkv) and jc virus (jcv) in ovarian tissues of women with ovarian carcinomas, borderline tumors, and benign conditions. | 2010 | 19766974 |
| mycoplasma genitalium-derived lipid-associated membrane proteins activate nf-kappab through toll-like receptors 1, 2, and 6 and cd14 in a myd88-dependent pathway. | mycoplasma genitalium is a leading pathogen of nongonoccocal chlamydia-negative urethritis, which has been implicated directly in numerous other genitourinary and extragenitourinary tract pathologies. the pathogenesis of infection is attributed in part to excessive immune responses. m. genitalium-derived lipid-associated membrane proteins (lamps) are a mixture of bacterial lipoproteins, exposed at the surface of mycoplasma, that are potent inducers of the host innate immune system. however, the ... | 2009 | 19793902 |
| life on arginine for mycoplasma hominis: clues from its minimal genome and comparison with other human urogenital mycoplasmas. | mycoplasma hominis is an opportunistic human mycoplasma. two other pathogenic human species, m. genitalium and ureaplasma parvum, reside within the same natural niche as m. hominis: the urogenital tract. these three species have overlapping, but distinct, pathogenic roles. they have minimal genomes and, thus, reduced metabolic capabilities characterized by distinct energy-generating pathways. analysis of the m. hominis pg21 genome sequence revealed that it is the second smallest genome among sel ... | 2009 | 19816563 |
| p110 and p140 cytadherence-related proteins are negative effectors of terminal organelle duplication in mycoplasma genitalium. | the terminal organelle is a complex structure involved in many aspects of the biology of mycoplasmas such as cell adherence, motility or cell division. mycoplasma genitalium cells display a single terminal organelle and duplicate this structure prior to cytokinesis in a coordinated manner with the cell division process. despite the significance of the terminal organelle in mycoplasma virulence, little is known about the mechanisms governing its duplication. | 2009 | 19829712 |
| idiopathic urethritis in young men in the united states: prevalence and comparison to infections with known sexually transmitted pathogens. | urethritis is the most common male reproductive tract disease syndrome; yet 20-50% of diagnosed cases have no defined etiology, and few population-level data exist on the prevalence or etiology of the syndrome. we estimated the prevalence of urethritis among young men in the united states and compared correlates of idiopathic cases to correlates of detected infections with sexually transmitted pathogens. | 2009 | 19837352 |
| the prevalence of urethral and rectal mycoplasma genitalium and its associations in men who have sex with men attending a genitourinary medicine clinic. | to determine the prevalence of rectal and urethral mycoplasma genitalium (mg) in men who have sex with men (msm) attending a genitourinary medicine clinic and to measure its associations with symptoms, clinical signs, sexual behaviour and concomitant sexually transmitted infections (sti). | 2010 | 19843536 |
| comparison of polymerase chain reaction and culture for detection of genital mycoplasma in clinical samples from patients with genital infections. | comparison of polymerase chain reaction (pcr) and culture for detection of genital mycoplasma (mycoplasma hominis, mycoplasma genitalium, and ureaplasma urealyticum) in clinical samples from patients with genital infections. | 2009 | 19882050 |
| an example of competitive inhibition in a monoplex real-time pcr as a cause of reduced fluorescent signal response. | | 2009 | 19900120 |
| current concepts in managing pelvic inflammatory disease. | the management of pelvic inflammatory disease (pid) has significantly changed during the last two decades. moreover, some recent bacterial findings have led to recent changes in this management. | 2010 | 19935421 |
| mycoplasma genitalium rapidly disseminates to the upper reproductive tracts and knees of female mice following vaginal inoculation. | mycoplasma genitalium is an emerging sexually transmitted infection and in women is associated with notable reproductive tract syndromes such as cervicitis, pelvic inflammatory disease, and infertility. investigations into the causal relationships of m. genitalium infections and clinical disease have been hindered largely by the lack of a well-established small-animal model of genital tract infection. to establish a murine model, female swiss webster mice were conditioned with either progesteron ... | 2010 | 19995897 |
| synthetic viruses: a new opportunity to understand and prevent viral disease. | rapid progress in dna synthesis and sequencing is spearheading the deliberate, large-scale genetic alteration of organisms. these new advances in dna manipulation have been extended to the level of whole-genome synthesis, as evident from the synthesis of poliovirus, from the resurrection of the extinct 1918 strain of influenza virus and of human endogenous retroviruses and from the restructuring of the phage t7 genome. the largest dna synthesized so far is the 582,970 base pair genome of mycopla ... | 2009 | 20010599 |
| failure to detect mycoplasma genitalium in the pharynges of female sex workers in japan. | to determine the prevalence of genital mycoplasmas and ureaplasmas in the pharynges of japanese female sex workers practicing fellatio on their clients, vaginal swabs and throat washings were collected from 403 female sex workers attending a clinic in kyoto, japan, for regular screening of gonococcal and chlamydial infections. neisseria gonorrhoeae, chlamydia trachomatis, mycoplasma genitalium, mycoplasma hominis, ureaplasma parvum, and ureaplasma urealyticum in vaginal and throat specimens were ... | 2009 | 20012733 |
| the association between mycoplasma genitalium and pelvic inflammatory disease after termination of pregnancy. | the prevalence and complications of mycoplasma genitalium and chlamydia trachomatis infections among women undergoing termination of pregnancy were studied in this nested case-control study at malmo university hospital, sweden, during 2003 to 2007. the study comprised 2079 women presenting for termination of pregnancy. forty-nine women with m. genitalium infection and 51 women with c. trachomatis infection, together with 168 negative control women, were evaluated. the prevalences of m. genitaliu ... | 2010 | 20015303 |
| prevalence of mycoplasma genitalium among hiv-infected men in são paulo city detected by realtime polymerase chain reaction. | genital mycoplasmas are natural inhabitants of the male urethra and are potentially pathogenic species playing an aetiological role in both genital infections and male infertility. this study aims to determine the presence of mycoplasma genitalium dna in urine samples of hiv-1-infected men in são paulo city. realtime polymerase chain reaction (pcr) was performed using the primers my-ins and mgso-2 and the taqman probe mgen-p1 as described previously. a total of 223 hiv-1-infected men were tested ... | 2010 | 20029063 |
| the bacterial essence of tiny symbiont genomes. | bacterial genomes vary in size over two orders of magnitude. the mycoplasma genitalium genome has historically defined the extreme small end of this spectrum, and has therefore heavily informed theoretical and experimental work aimed at determining the minimal gene content necessary to support cellular life. recent genomic data from insect symbionts have revealed bacterial genomes that are incredibly small-two to four times smaller than m. genitalium-and these tiny genomes have raised questions ... | 2010 | 20044299 |
| correlates of cervical mycoplasma genitalium and risk of preterm birth among peruvian women. | mycoplasma genitalium is associated with cervicitis and pelvic inflammatory disease in nonpregnant women. we investigated associations between cervical m genitalium, demographic and behavioral risk factors for sexually transmitted infection and preterm birth among low-income peruvian women. | 2010 | 20051932 |
| a case-control study of men with non-gonococcal urethritis at auckland sexual health service: rates of detection of mycoplasma genitalium. | previous studies have identified mycoplasma genitalium as a cause of urethritis in men. as there is no new zealand data, a case-control study was conducted to determine whether this organism is a significant cause of urethritis in men presenting to auckland sexual health service. | 2010 | 20152101 |
| [vaginal ectopia of urethra as a cause of chronic recurrent cystitis in women: diagnosis and treatment]. | ectopia of the urethral opening (female hypospadia) often causes chronic recurrent cystitis in women because of a retrograde delivery of urogenital infection from the introitus and vagina to the short wide urethra and urinary bladder. etiologically, cystitis develops due to pathogenic vaginal microflora: chlamydia trachomatis, mycoplasma genitalium, ureaplasma, urealyticum, gardnerella vaginalis, candida, e. coli and other gram-negative bacteria. in 64 female patients aged 16-57 years with femal ... | 2009 | 20209864 |
| cloning whole bacterial genomes in yeast. | most microbes have not been cultured, and many of those that are cultivatable are difficult, dangerous or expensive to propagate or are genetically intractable. routine cloning of large genome fractions or whole genomes from these organisms would significantly enhance their discovery and genetic and functional characterization. here we report the cloning of whole bacterial genomes in the yeast saccharomyces cerevisiae as single-dna molecules. we cloned the genomes of mycoplasma genitalium (0.6 m ... | 2010 | 20211840 |
| mycoplasma genitalium symptoms, concordance and treatment in high-risk sexual dyads. | the objective of this study was to determine the prevalence and concordance of mycoplasma genitalium (mg) among mexican american and african american women and their male sexual partners. secondary objectives were to determine symptoms of mg infection and persistence of mg after antibiotic therapy. heterosexual couples were tested for mg and interviewed separately regarding symptoms and behavioural/epidemiologic variables at baseline, six and 12 months. the overall prevalence of mg among women a ... | 2010 | 20215621 |
| viral and bacterial aetiologies of male urethritis: findings of a high prevalence of epstein-barr virus. | male urethritis is one of the most common sexually transmitted infections (stis). however, the aetiology is still unclear in many cases. in this study the prevalences of epstein-barr virus (ebv), herpes simplex virus type 1 (hsv-1), hsv-2, cytomegalovirus (cmv), adenovirus, chlamydia trachomatis, mycoplasma genitalium and ureaplasma urealyticum (including subtyping) were investigated. samples from 112 male sti attendants with microscopically verified urethritis and from a control group of 103 me ... | 2010 | 20215624 |
| tandem repeat coupled with endonuclease cleavage (trec): a seamless modification tool for genome engineering in yeast. | the complete synthetic mycoplasma genitalium genome ( approximately 583 kb) has been assembled and cloned as a circular plasmid in the yeast saccharomyces cerevisiae. attempts to engineer the cloned genome by standard genetic methods involving the ura3/5-fluoroorotic acid (5-foa) counter-selection have shown a high background of 5-foa resistant clones derived from spontaneous deletions of the bacterial genome maintained in yeast. here, we report a method that can seamlessly modify the bacterial ... | 2010 | 20228123 |
| [chlamydia a common cause of miction problems]. | | 2010 | 20297580 |
| [chlamydia and genital mycoplasma: epidemiology and risks]. | | 2010 | 20297583 |
| scientists playing god! | | 2009 | 20329365 |
| diagnosis and treatment of urethritis in men. | symptoms of urethritis in men typically include urethral discharge, penile itching or tingling, and dysuria. a diagnosis can be made if at least one of the following is present: discharge, a positive result on a leukocyte esterase test in first-void urine, or at least 10 white blood cells per high-power field in urine sediment. the primary pathogens associated with urethritis are chlamydia trachomatis and neisseria gonorrhoeae. racial disparities in the prevalence of sexually transmitted infecti ... | 2010 | 20353145 |
| [a case of fitz-hugh-curtis syndrome in a male]. | fitz-hugh-curtis syndrome has been described as focal perihepatitis accompanying pelvic inflammatory disease caused by neisseria gonorrhea and chlamydia trachomatis. the highest incidence occurs in young, sexually active females. however, the syndrome has been reported to occur infrequently in males, according to the foreign literature. the predominant symptoms are right upper quadrant pain and tenderness, and pleuritic right sided chest pain. the clinical presentation is similar in men and wome ... | 2010 | 20357533 |
| mycoplasma genitalium among young, urban pregnant women. | as the consequences of mycoplasma genitalium in pregnant women are unknown, we examined the relationship between prenatal m. genitalium infection and sab. | 2010 | 20379360 |
| differential association of ureaplasma species with non-gonococcal urethritis in heterosexual men. | to assess the role of ureaplasma urealyticum and ureaplasma parvum in patients with non-gonococcal urethritis (ngu) using specimens from a previously reported study of ngu. | 2010 | 20460265 |
| genomics. synthetic genome brings new life to bacterium. | | 2010 | 20488994 |
| multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens. | the objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mpcr) based reverse line blot (rlb) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (neisseria gonorrhoeae, chlamydia trachomatis, ureaplasma urealyticum, ureaplasma parvum, mycoplasma genitalium, mycoplasma hominis and trichomonas vaginalis). species-specific biotin-labelled primer pairs were used in a single mpcr ... | 2010 | 20498099 |
| ureaplasma urealyticum is significantly associated with non-gonococcal urethritis in heterosexual sydney men. | we investigated the prevalence of various genital organisms in 268 men with (cases) and 237 men without (controls) urethral symptoms/signs (urethral discharge, dysuria and/or urethral irritation) from two sexual health clinics in sydney between april 2006 and november 2007. the presence of urethral symptoms/signs was defined as non-gonococcal urethritis (ngu) for this study. specific aims were to investigate the role of ureaplasma urealyticum in ngu and the prevalence of mycoplasma genitalium in ... | 2010 | 20498103 |
| etiology and sti/hiv coinfections among patients with urethral and vaginal discharge syndromes in south africa. | this study was undertaken to establish the etiology of the male urethral discharge (muds) and vaginal discharge (vds) syndromes, to determine the prevalence of other sexually transmitted infections (sti) and human immunodeficiency virus (hiv) coinfections, and to examine associations between stis and hiv serostatus among sti patients in south africa. | 2010 | 20502394 |
| emergence of clinical strains of mycoplasma genitalium harbouring alterations in parc associated with fluoroquinolone resistance. | surveillance for antimicrobial resistance in mycoplasma genitalium clinical strains is extremely limited as culturing of strains from clinical specimens is still difficult. we therefore conducted a non-cultural assessment of fluoroquinolone resistance of m. genitalium clinical strains by analysing the quinolone-resistance determining regions (qrdrs) of the gyra and parc genes. the qrdrs amplified from m. genitalium dna taken from urine specimens of 28 men with non-gonococcal urethritis positive ... | 2010 | 20580532 |
| molecular cloning, expression, and characterization of a ca2+-dependent, membrane-associated nuclease of mycoplasma genitalium. | in this study, we identified and characterized the enzymatic properties of mg_186, a calcium-dependent mycoplasma genitalium nuclease. mg_186 displays the hallmarks of nucleases, as indicated by its amino acid sequence similarity to other nucleases. we cloned, uga corrected, expressed, purified, and demonstrated that recombinant mg_186 (rmg_186) exhibits nuclease activity similar to that of typical sugar-nonspecific endonucleases and exonucleases. biochemical characterization indicated that ca2+ ... | 2010 | 20639320 |
| pelvic inflammatory disease. | pelvic inflammatory disease (pid) is an infection-caused inflammatory continuum from the cervix to the peritoneal cavity. most importantly, it is associated with fallopian tube inflammation, which can lead to infertility, ectopic pregnancy, and chronic pelvic pain. the microbial etiology is linked to sexually transmitted microorganisms, including chlamydia trachomatis, neisseria gonorrheae, mycoplasma genitalium, and bacterial vaginosis-associated microorganisms, predominantly anaerobes. pelvic ... | 2010 | 20664404 |