| protein patterns of streptomyces griseus strains during development. | protein patterns from mycelial extracts of streptomyces griseus strains no. 45h and no. 52-1 were studied by one dimensional gradient page with 20 cm run. the results are reproducible, the protein patterns are the same for the same developmental stage in a given strain. there are well-defined characteristic changes of the protein patterns during the life cycle. the proteins of spores show conspicuous differences compared to protein patterns of the old (72 h) mycelia in the same culture. there is ... | 1984 | 6428069 |
| pleiotropic effects of a butyrolactone-type autoregulator on mutants of streptomyces griseus blocked in cytodifferentiation. | mutants of streptomyces griseus blocked in cytodifferentiation regained their capacity to form differentiated mycelia and/or anthracycline pigments in the presence of butyrolactone-type autoregulatory effectors such as trans-2-(6'-methylheptanol-1'-yl)-3-hydroxymethyl-4-butanolide+ ++. in the pertinent indicator strains, the effect has been correlated with the increase of lipid synthesis, with changes in the composition of lipid fraction and with the restoration of the production of neutral prot ... | 1984 | 6432945 |
| studies on streptomyces griseus protease. i. separation of dfp-reacting enzymes and purification of one of the enzymes. | | 1968 | 5636371 |
| a piecemeal description of streptomyces griseus (krainsky) waksman and henrici. | | 1968 | 5640225 |
| nocardia dassonvillei, a macroscopic replica of streptomyces griseus. | | 1968 | 5640226 |
| effect of trypsin inhibitors of streptomyces griseus cal. on blood clotting and fibrinolysis. | | 1984 | 6436171 |
| resistance of alkaline phosphatase of human serum to the protease of streptomyces griseus. | | 1968 | 5657464 |
| the metabolism of scyllo-inositol in streptomyces griseus. | | 1968 | 5683534 |
| human parotid size polymorphism (ps): characterization of two allelic products, ps 1 and 2, by limited proteolysis. | the allelically determined human salivary proteins, ps 1 and 2, were purified on sodium dodecyl sulfate gels, eluted, and compared by limited proteolysis with streptomyces griseus protease vi, bacillus subtilis protease vii, and staphylococcus aureus protease v8. prior dansylation of the ps isoproteins facilitated visualization of the peptides. digestion patterns indicate considerable homology between the ps isoproteins and support the conclusion [azen, a. e., and denniston, c. (1980). biochem. ... | 1983 | 6344862 |
| comparison of protein patterns of streptomyces griseus spores from surface and submerged cultures. | protein patterns of conidia produced by a streptomyces griseus strain in submerged cultures and on solid media were compared. cell-free extracts (30 000 x g supernatant) were prepared and analyzed on gradient sds polyacrylamide gels. the protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way. | 1984 | 6441361 |
| [extracellular serine proteinase a of streptomyces rutgersensis]. | extracellular serine proteinase of streptomyces rutgersensis (proteinase a) has been isolated by affinity chromatography on bacitracin-sepharose in the presence of na2edta. the proteolytic activity of the enzyme is completely suppressed by specific inhibitors of serine proteinases, phenylmethylsulfonylfluoride and diisopropylfluorophosphate, but is insensitive to na2edta and sodium p-hydroxymercuribenzoate. the enzyme activity towards hemoglobin is maximal at ph 9-10, the maximal stability is ob ... | 1983 | 6354278 |
| phospholipase b from the plasma membrane of saccharomyces cerevisiae. separation of two forms with different carbohydrate content. | two forms of phospholipase b could be solubilized from the plasma membrane of saccharomyces cerevisiae, separated by gel filtration with sephacryl s-300 and identified by sds-polyacrylamide gel electrophoresis as glycoproteins of the apparent molecular weights of about 220 000 (phospholipase b1) and 145 000 (phospholipase b2). the enzymes are very similar in respect to their catalytic properties. both forms converted lysophosphatidylcholine to diacylphosphatidylcholine and unesterified fatty aci ... | 1984 | 6380591 |
| examination of protein sequence homologies: i. eleven escherichia coli l7/l12-type ribosomal "a" protein sequences from eubacteria and chloroplast. | seven complete and four partial sequences of escherichia coli l7/l12-type ribosomal "a" proteins obtained from various bacteria (e. coli, bacillus subtilis, micrococcus lysodeikticus, rhodopseudomonas spheroides, desulfovibrio vulgaris, streptomyces griseus, bacillus stearothermophilus, clostridium pasteurianum, arthrobacter glacialis, and vibrio costicola) and spinach chloroplast have been reexamined using a computer program that searches for homologous tertiary structures. comparison matrices ... | 1984 | 6443314 |
| phosphoenolpyruvate-dependent phosphorylation site in enzyme iiiglc of the escherichia coli phosphotransferase system. | enzyme-iiiglc is part of the glucose phosphotransferase system of escherichia coli and salmonella typhimurium and is phosphorylated by phosphoenolpyruvate in a reaction requiring enzyme i (phosphoenolpyruvate-protein phosphotransferase), and the histidine-containing phospho-carrier protein hpr. in this paper we report the isolation of iiiglc from e. coli and the characterization of the active center. alkaline hydrolysis of [32p]p-iiiglc and chromatography of the hydrolysate suggested that the ph ... | 1984 | 6383826 |
| [growth and antimicrobial activity of an association of an actinomycete and a green alga]. | during joint cultivation of the actinomycetes streptomyces griseus (strains 65 and 744) isolated from the soil and the green algae chlorella vulgaris larger amount of biomass as compared with solitary axenic culture have been shown. the relation of biomass of actinomycetes s. griseus strain 65 and s. griseus strain 744 and algae in the lichen-like experimentally formed thallom make up 42:1 and 40:1 relatively, i. e. the mass of actinomycetes forms 97-98% from the mass of thalloms. actinomycetes ... | 1983 | 6405815 |
| [effect of preservation conditions on the viability and activity of streptomyces griseus--producer of the antibiotic grizin]. | strain aurigenetics- 1552 of str. griseus producing grisin used as a feed additive was obtained in the course of stepped selection. it is deposited at the central collection of industrial microorganisms of the ussr and designated as strain s-248. the strain was stored under varying conditions and their effect on its viability, morphological variation and antibiotic potency was studied. investigation of the strain variation with respect to the cultural and morphological properties and the antibio ... | 1983 | 6407387 |
| inhibition of metalloendopeptidases by 2-mercaptoacetyl-dipeptides. | a series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the n-hydroxysuccinimide ester of s-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with naoh in aqueous methanol and acidification with hcl. thus, the 2-mercaptoacetyl derivatives of l-phenylalanyl-l-leucine, l-leucyl-l-phenylalanine and l-leucyl-d-phenylalanine were prepared. the first two compounds inhibit e ... | 1983 | 6413206 |
| biochemical investigations of streptomycin production by streptomyces griseus. | | 1983 | 6414994 |
| metabolism of cinnamic, p-coumaric, and ferulic acids by streptomyces setonii. | streptomyces setonii strain 75vi2 was grown at 45 degrees c in liquid media containing yeast extract and trans-cinnamic acid, p-coumaric acid, ferulic acid, or vanillin. gas chromatography, thin-layer chromatography, and mass spectrometry showed that cinnamic acid was catabolized via benzaldehyde, benzoic acid, and catechol; p-coumaric acid was catabolized via p-hydroxybenzaldehyde, p-hydroxybenzoic acid, and protocatechuic acid; ferulic acid was catabolized via vanillin, vanillic acid, and prot ... | 1983 | 6661696 |
| relation of anabolic-catabolic glucose utilization in growth-limited cultures of streptomyces griseus. | phenotypically different submerged mycelium conserves had been produced from a spore conserve of the hp-strain streptomyces griseus and proofed in a product formation culture as a test system. the phenotypical characters induced on the base of the genotype proved in a cultivation cycle during 30-34 reduplications of the biomass constant. employing the hp phenotype we investigated the possibility of economizing the substrate turnover by utilizing the anabolic potential for the synthesis of second ... | 1983 | 6415934 |
| characterization of antigens on mosquito midgut stages of plasmodium gallinaceum. i. zygote surface antigens. | we have defined the surface protein antigens on plasmodium gallinaceum zygotes using radioiodination methods and rabbit anti-zygote serum which blocks transmission of the parasite to aedes aegypti mosquitoes. fifteen protein bands (1-15) in the molecular weight range of 40 000-240 000 and one band at the bromophenol blue dye marker were labelled by the lactoperoxidase and iodogen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril) methods. the localization of these radioiodinated components ... | 1983 | 6683783 |
| effect of the autoregulator from streptomyces griseus ja 5142 on surface cultures of blocked mutant zimet 43682. | zero time addition of the autoregulator (l-factor) from s. griseus (lkm+amy+) to surface cultures of its bald mutant zimet 43 682 (amy-lkm-) restored the capacity to form both anthracycline-type antibiotic leukaemomycin and aerial mycelium. the pertinent mycelia displayed the same growth rate and cellular levels of nucleic acids as the asporogeneous phenotype but the composition of fatty acids and phospholipids as well as the ratio of cytochromes b and c were altered. these differences indicate ... | 1983 | 6415935 |
| secondary structure and phylogeny of staphylococcus and micrococcus 5s rrnas. | nucleotide sequences of 5s rrnas from four bacteria, staphylococcus aureus smith (diffuse), staphylococcus epidermidis atcc 14990, micrococcus luteus atcc 9341 and micrococcus luteus atcc 4698, were determined. the secondary structural models of s. aureus and s. epidermidis sequences showed characteristics of the gram-positive bacterial 5s rrna (116-n type [h. hori and s. osawa, proc. natl. acad. sci. u.s.a. 76:381-385, 1979]). those of m. luteus atcc 9341 and m. luteus atcc 4698 together with t ... | 1984 | 6735981 |
| [use of the protoplast fusion method in the selection of streptomyces griseus--the producer of the streptothricin antibiotic grisin]. | an effective method for protoplast fusion in s. griseus producing grisin was developed. the method requires the use of polyethylene glycol with a molecular weight of 1000. it was demonstrated that protoplasts formed most effectively in this organism, when the mycelium of the streptomycete previously treated with ultrasound in the process of its growth was used for the treatment with lysozyme. the efficacy of protoplast regeneration in the strains with the use of the modified hypertonic medium r2 ... | 1983 | 6419670 |
| refined crystal structure of the molecular complex of streptomyces griseus protease b, a serine protease, with the third domain of the ovomucoid inhibitor from turkey. | we have determined the crystal structure of the molecular complex between streptomyces griseus protease b (sgpb), a bacterial serine protease, and the third domain of the ovomucoid inhibitor from turkey. restrained-parameter least-squares refinement of the structure with the 1.8-a intensity data set has resulted in an r factor of 0.125. the carbonyl carbon atom of the reactive bond between leu-18 and glu-19 in the inhibitor lies at a distance of 2.71 a from the o gamma atom of the nucleophilic s ... | 1982 | 6750612 |
| cloning and expression of a p-aminobenzoic acid synthetase gene of the candicidin-producing streptomyces griseus. | 4.5-kb bamhi fragments of dna coding for p-aminobenzoic acid (paba) synthetase from the candicidin-producing streptomyces griseus imru 3570 and from a sulphonamide resistant mutant of it were cloned on the plasmid vector pij41 into streptomyces lividans 66. the cloned dna restored prototrophy to a pab auxotroph of s. lividans; when the s. griseus donor was a sulphonamide resistant, paba-overproducing mutant, the s. lividans clone was sulphonamide resistant as well as pab+. sub-cloning the 4.5-kb ... | 1983 | 6420235 |
| isolation and characterization of pock-forming plasmids for streptomyces griseus from soil actinomycetes. | thirty independent actinomycetes strains carrying plasmids were isolated from soil. these plasmids were purified as cccdna by cscl-etbr equilibrium density-gradient centrifugation. plasmids that induce "pocks", namely formation of circular zones of sporulation-inhibition, were selected by protoplast transformation of streptomycin-producing strain, streptomyces griseus atcc10137. six pock-forming plasmids, poa7, poa11, poa15, poa23, poa29 and poa30, were obtained, and their cleavage maps, transfo ... | 1983 | 6420237 |
| studies on ti-2 trypsin inhibitor of streptomyces griseus. | the trypsin inhibitor ti-2 produced by streptomyces griseus cal in a medium, containing beef extract and peptone as the nitrogen source, was purified by ammonium sulfate precipitation and gel filtration. amino acid analysis showed that it contained hydroxyproline and carbohydrate whereas tyrosine, tryptophan, and cystine were absent. the composition of the inhibitor protein thus showed a similarity to collagen. growth of the organism in a medium with gelatin as the sole nitrogen source resulted ... | 1983 | 6421469 |
| [carboxypeptidase t--intracellular carboxypeptidase of thermoactinomycetes--a distant analog of animal carboxypeptidase]. | carboxypeptidase t, an extracellular carboxypeptidase from thermoactinomyces sp. was isolated and purified by affinity chromatography on bacitracin adsorbents. the enzyme homogeneity was established by sds electrophoresis (mr = 38 000) and isoelectrofocusing in paag (pi 5.3). carboxypeptidase t reveals a mixed specificity in comparison with pancreatic carboxypeptidases a and b and cleaves with nearly the same efficiency the peptide bonds formed by the c-terminal residues of basic and neutral hyd ... | 1984 | 6424730 |
| partial characterization of thymocyte-activating factor derived from mdp-stimulated guinea pig fibroblasts. | the activity of fibroblast-derived thymocyte activating factor (ftaf) of the guinea pig was measured, and the factor was partially characterized. the ftaf activity was heat labile, and destroyed by treatment with trypsin, chymotrypsin, and streptomyces griseus protease, suggesting the protein nature of ftaf. ftaf bound to deae-sepharose cl-6b in tris-hcl buffer at ph 8.0, and was eluted with 0.1-0.2 m nacl. ftaf was absorbed with blue sepharose cl-6b. the factor bound to a hydroxylapatite column ... | 1984 | 6335219 |
| a triglobular model for the polypeptide chain of aspartokinase i-homoserine dehydrogenase i of escherichia coli. | limited proteolysis of aspartokinase i-homoserine dehydrogenase i from escherichia coli by type vi protease from streptomyces griseus yields five proteolytic fragments, three of which are dimeric, the other two being monomeric. one of the monomeric fragments (27 kilodaltons) exhibits residual aspartokinase activity, while the second one (33 kilodaltons) possesses residual homoserine dehydrogenase activity. the smallest of the dimeric species (2 x 25 kilodaltons) is inactive; the two other dimers ... | 1983 | 6338915 |
| [changes in the actinophage sensitivity and resistance of a culture of actinomyces griseus and its variants]. | susceptibility and resistance of the culture of actinomyces griseus 15 and its variants to actinophages was studied. variants different from the parent culture in their susceptibility or resistance to certain phages can arise as the result of spontaneous variability and lysogenization. the culture of actinomyces griseus 15 resistant to nine polyphates was found to be able to adsorb them. the mycelium of act. griseus 15 was shown to contain receptors for a page to which it was susceptible differe ... | 1980 | 6771500 |
| inducers of both cytodifferentiation and anthracycline biosynthesis of streptomyces griseus and their occurrence in actinomycetes and other microorganisms. | many taxonomically different strains of actinomycetes as well as several other procaryotic and eucaryotic microorganisms were shown to produce inducers of cytodifferentiation and anthracycline biosynthesis being active towards blocked mutant zimet 43682 of streptomyces griseus. 37 out of 40 strains of the species of s. griseus (92.5%) yielding different kinds of antibiotics were able to induce both the formation of aerial mycelium in the indicator strain zimet 43682 concomitant with the biosynth ... | 1984 | 6426178 |
| unstable genetic determinant of a-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. | we cloned a dna fragment directing synthesis of a-factor from the total cellular dna of streptomycin-producing streptomyces bikiniensis on the plasmid vector pij385 . introduction of the recombinant plasmid ( pafb1 ) into a-factor-deficient s. bikiniensis and streptomyces griseus mutants led to a-factor production in the host cells, as a result of which streptomycin production, streptomycin resistance, and spore formation of these mutants were simultaneously restored. the plasmid pafb1 also comp ... | 1984 | 6427181 |
| regulation by aromatic amino acids of the biosynthesis of candicidin by streptomyces griseus. | the biosynthesis by streptomyces griseus of candicidin, an aromatic polyene macrolide antibiotic, was inhibited by l-tryptophan, l-phenylalanine and, to a lesser degree, by l-tyrosine. a mixture of the three aromatic amino acids inhibited candicidin biosynthesis to a greater extent than did each amino acid separately. l-tryptophan strongly inhibited the incorporation of the labelled precursors propionate or 4-aminobenzoic acid into candicidin. incorporation of propionate into candicidin was 50% ... | 1980 | 6775046 |
| critical evaluation of comparative model building of streptomyces griseus trypsin. | the x-ray crystal structure of streptomyces griseus trypsin has been solved and refined at 1.7-a resolution. the structure of this protein had been predicted in two models on the basis of its expected homology to structures of bovine trypsin and other pancreatic serine proteases [jurásek, l., olafson, r.w., johnson, p., & smillie, l.b. (1976) miami winter symp. 11, 93-123; greer, j. (1981) j. mol. biol. 153, 1027-1042]. an evaluation of these models in light of the known structure demonstrates t ... | 1984 | 6442164 |
| field desorption mass spectrometry in structural studies of polyene macrolide antibiotics: isolation and early identification of a pentaene macrolide antibiotic. | isolation of a pentaene macrolide antibiotic (nsc-277813) from streptomyces griseus (fcrc-21) fermentation broth is described. using field desorption mass spectrometry and high resolution field desorption mass spectrometry on the intact and derivatized antibiotic and degradation products, the antibiotic was identified as fungichromin. the application of field desorption mass spectrometry in the identification of polyene antibiotics is discussed. | 1980 | 6775705 |
| [regulators of differentiation in streptomyces cyaneofuscatus]. | streptomyces cyaneofuscatus prl 1642 producing valinomycin was shown to synthesize bioregulators of differentiation similar to a factor. the regulators stimulate spore formation and streptomycin synthesis in a streptomyces griseus 1439 mutant deficient in a factor. some s. cyaneofuscatus mutants respond to a factor addition into the medium by an increase in valinomycin synthesis and a change in morphological properties. the regulators from s. cyaneofuscatus are more effective toward mutants of t ... | 1984 | 6442388 |
| plasmid deoxyribonucleic acid replication in streptomyces griseus. | a series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from streptomyces griseus was obtained. based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in s. griseus is proposed. | 1981 | 6783620 |
| biosynthetic studies of amphotericins, candicidin and nystatin by means of mutation. | a novel mutant strain designated as streptomyces nodosus, ndmc-034 was selected from the mutation of streptomyces nodosus, imru 3694. this mutant was characterized by delayed sporulation, the incapability of producing the melanoid pigment either in the production medium or on yd agar containing 1% yeast extract and 1% dextrose, and the increased yield of amphotericin a as compared to its parent strain. in addition, the biosynthesis of amphotericin a by this mutant seems to be sensitive to temper ... | 1984 | 6443786 |
| [isolation of streptomyces griseus mutants by blocking streptomycin synthesis and research on their proteolytic activity]. | | 1983 | 6443858 |
| control by phospho-adenosinediphospho-ribose of nadp-dependent isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in streptomyces griseus. | the metabolic function of nad(p)-glycohydrolase in the streptomycin-producing streptomyces griseus was investigated. phospho-adenosinediphospho-ribose, the product of nad(p)-glycohydrolase reaction was shown to interfere as a competitive inhibitor not only with the glucose-6-phosphate dehydrogenase (voronina et al. 1978) but also with the nadp-dependent isocitrate and 6-phospho-gluconate dehydrogenases. inhibition kinetics were studied with isocitrate dehydrogenase from pig heart and 6-phosphogl ... | 1980 | 6784352 |
| characterization of phospholipase a in streptomyces griseus. | | 1980 | 6785216 |
| [isolation of protease complexes from streptomyces griseus by ultrafiltration]. | the possibility of concentrating proteases from streptomyces griseus by the ultrafiltration technique using soviet membranes of the mb-i-iv type was explored. this method allowed a 3-10 fold reduction of the native solution of enzymes and their simultaneous purification from by-products. | 1981 | 6787580 |
| [genetic recombination and chromosome mapping of streptomyces griseus 773]. | | 1983 | 6443859 |
| microbial transformation of n-methylcolchiceinamide. | seventy-seven microorganisms were examined for their ability to metabolize the antineoplastic agent n-methylcolchiceinamide, an analog of colchicine. five streptomycetes exhibited significant metabolism, and streptomyces griseus nrrl b-599 completely converted the substrate to three metabolites. in preparative-scale studies, n-dealkylation resulted in the production of colchiceinamide, the major metabolite (65%), which was characterized by chemical, spectroscopic, and chromatographic comparisons ... | 1981 | 6787979 |
| [comparison of different methods for the identification of groups of streptococci]. | the streptococci, isolated from 500 mucus-pharyngeal tampons, have been tested, for a group identification, by means of four different techniques in order to value the specificity and reliability in comparison with more traditional and, sometimes, more complex tests; such as maxted and lancefield. the most suitable method for routine researchers of microbiology laboratories is the one based on the extraction, by means of enzyme obtained from streptomyces griseus, of streptococci antigens before ... | 1983 | 6626338 |
| stimulation of rat peritoneal mast cell migration by tumor-derived peptides. | the accumulation of mast cells is characteristic of a number of pathological states. we demonstrate here the directional motility of mast cells in vitro in response to tumor-derived peptides. rat peritoneal mast cells were isolated on percoll gradients and maintained in serum-free medium containing transferrin, albumin, soybean lipid, and cholesterol. the isolated mast cells migrated under agarose in response to medium conditioned by any of eight tumor cell lines but not to medium conditioned by ... | 1983 | 6640537 |
| c-19393 s2 and h2, new carbapenem antibiotics. ii. isolation and structures. | two new beta-lactam antibiotics, c-19393 s2 (1) and h2 (2), were isolated from the culture filtrate of streptomyces griseus subsp. cryophilus nov. subsp. the structures were determined by spectral analysis as shown in fig. 2. the antibiotics have broad antimicrobial activity and strongly inhibit beta-lactamases. the minor product (2) is more stable than cephalosporin c in aqueous solution. | 1980 | 6788740 |
| a new polyene with antimycotic activity produced by a streptomycete from the rhizosphere of an indoor plant. | from the rhizosphere of a potted sanseviera trifasciata, a streptomyces griseus strain with a broad antifungal activity was isolated; in the soil of this plant, a sole hyphomycete, aspergillus niger, was found in pure culture at 37 degrees c. this streptomycete produced a polyene, belonging to the pentaene group. it was found to inhibit, in vitro, the growth of all the 123 strains tested; the strains belonging to the genera aspergillus, cryptococcus, candida, trichophyton, epidermophyton, micros ... | 1983 | 6681974 |
| structure of the major oligosaccharides in the fusion glycoprotein of newcastle disease virus. | the fusion glycoprotein (f0) was isolated from newcastle disease virus (ndv) particles metabolically labelled with [2-3h]mannose; it was successively digested with protease and with endo-beta-n-acetylglucosaminidase from streptomyces griseus. in this manner, the majority of the oligosaccharides in ndv f0 could be liberated. after reduction with nabh4, they were separated by high-performance liquid chromatography, and were subjected to structural analysis. using micromethylation/capillary gas chr ... | 1984 | 6698016 |
| directed biosynthesis of new indolmycins. | tryptophan in a concentration of 0.4 microgram/ml increased the production of indolmycin by 37%. the lipophilic character of indolmycin was reduced via directed biosynthesis by substituting the aromatic ring system with a methoxy or hydroxy group in the 5-position of the antibiotic. this substitution was achieved by the addition of the corresponding tryptophan and indole precursors to a growing culture of streptomyces griseus atcc 12648. the more hydrophilic indolmycin derivatives displayed a mo ... | 1981 | 6792175 |
| [new type of sporulation regulation and streptomycin biosynthesis in secondary streptomyces griseus mutants]. | | 1981 | 6792249 |
| formation of a reactive iminium derivative by enzymatic and chemical oxidations of 16-o-acetylvindoline. | 16-o- acetylvindoline (1a) was oxidatively transformed into an iminium derivative (2a) by copper oxidases (laccase and human ceruloplasmin), an unknown enzyme system(s) of streptomyces griseus, and the chemical oxidizing agent 2,3-dichloro-5,6- dicyano -1,4-benzoquinone ( ddq ). the iminium derivative (2a) was isolated from enzymatic and chemical oxidation mixtures and was identified by spectral and chemical techniques. reduction of the iminium compound with sodium borodeuteride provided monodeu ... | 1984 | 6737416 |
| complete amino acid sequence of streptokinase and its homology with serine proteases. | the complete amino acid sequence of streptokinase has been determined by automated edman degradation of its cyanogen bromide and proteolytic fragments. the protein consists of 415 amino acid residues. sequence microheterogeneity was found at two positions. the nh2-terminal 245 residues of streptokinase are homologous to the sequences of several serine proteases including bovine trypsin and streptomyces griseus proteases a and b. the sequence alignment suggests that the active-site histidine-57 h ... | 1982 | 6760891 |
| studies on prekallikrein of bovine plasma. ii. activation of prekallikrein with proteinases and properties of kallikrein activated by bovine hageman factor. | activation of bovine plasma prekallikrein was investigated with several proteinases. highly purified bovine plasma prekallikrein was rapidly activated to kallikrein [ec 3.4.21.8] by bovine activated hageman factor, trypsin [ec 3.4.21.4] and pronase p (proteinases from streptomyces griseus) and more gradually by papain [ec 3.4.22.2] and ficin [ec 3.4.22.3]. activation of prekallikrein was also observed with bovine plasmin [ec 3.4.21.7], but not with bovine clotting factors xa (stuart factor) [ec ... | 1980 | 6766924 |
| studies on trypsin inhibitors of streptomyces griseus cal. | | 1981 | 6800933 |
| proline transport increases growth efficiency in salt-stressed streptomyces griseus. | streptomyces griseus synthesizes proline for osmoregulation under salt stress. uptake of exogenous [14c]proline and internal synthesis of proline were quantified in cells growing at salt concentrations from 0 to 1 m nacl. externally supplied proline accounted for an increased proportion of the intracellular pool of free proline as salt concentration was increased, but neither the concentration nor the composition of the internal amino acid pool was substantially altered by supply of exogenous pr ... | 1984 | 6433794 |
| structure of the oligosaccharides sensitive to endo-beta-n-acetylglucosaminidase h in the glycoprotein of friend murine leukemia virus. | the surface glycoprotein (mixture of isoglycoproteins with mr 69 000 and 71 000) was isolated from the particles of friend murine leukemia virus, and was successively digested with protease and with endo-beta-n-acetylglucosaminidase from streptomyces griseus. roughly 20% (w/w) of the carbohydrates in this glycoprotein were thus released, and they were fractionated by high-performance liquid chromatography after reduction with kb3h4/nabh4. the radioactive oligosaccharitol fractions obtained were ... | 1984 | 6434306 |
| [interspecies recombination in streptomyces griseus, streptomyces olivaceus and streptomyces coelicolor]. | | 1982 | 6803104 |
| [circular permutation of dna molecules of streptomyces griseus kr actinophage pg2]. | dna molecules of phage pg2 have terminal repetitions and are partially circularly permuted. this was established by denaturation self-reannealing experiments and analysis of the distribution of denaturation loops in partially denatured pg2 dna molecules. the genome size is 40.8 kb, terminal repetitions are of about 2.2%, the degree of permutation is 35%. on the basis of the data on dna melting temperature the content of gc pairs was found equal to 65%. | 1982 | 6803124 |
| griseochelin, a novel carboxylic acid antibiotic from streptomyces griseus. | griseochelin, c33h60o7, isolated from an asporogenous strain of streptomyces griseus represents a novel carboxylic acid antibiotic. the metabolite, which is active against gram-positive bacteria, forms water-insoluble salts with mono- and divalent cations and binds alkaline-earth metal ions specifically in 2:1 (x2m) stoichiometry. detailed spectral (ir, ms and nmr) studies provide full characterization of its constitution featuring a carboxylic acid function, a substituted tetrahydropyran ring, ... | 1984 | 6434502 |
| acyl chains of phospholipids and their variation with age in streptomyces griseus. | | 1981 | 6805427 |
| a screening method for autoregulators of anthracycline-producing streptomycetes. | a novel method was proposed for screening of autoregulators by anthracycline-producing strains of streptomyces griseus. by means of a modified cosynthesis procedure, an indicator strain was detected capable of producing both leukaemomycin and aerial mycelium only in the presence of specific inducing factors (autoregulators) which were produced not only by anthracycline-yielding organisms and their mutants, but also by a streptomycin-producing strain of streptomyces griseus as well as by its non- ... | 1982 | 6806999 |
| biochemical characteristics of non-streptomycin-producing mutants of streptomyces griseus. ii. lipids and fatty acid composition of vegetative mycelia. | five non-streptomycin-producing non-aerial-mycelium-forming mutants (str-amy-) of streptomyces griseus obtained either by spontaneous degeneration or during continuous cultivation of the high-producing aerial-mycelium-forming parent strain hp (str+amy+) were checked with regard to the composition of mycelial lipid material. all the str-amy- derivatives differed from their ancestor strain hp by an increased ration of 12-methyltetradecanoic acid (ac15:0) to isopalmitic acid (ic16:0) during growth ... | 1982 | 6807000 |
| modification by genetic changes of the pleiotropic interference of butyrolactone-type autoregulators with differentiation of streptomyces griseus. | two series of aerial-mycelium-negative (amy-), anthracycline-nonproducing (ant-) mutants were obtained from ancestral amy+ant+ strains of s. griseus: a) derivatives represented by the met- strain 39 which could not differentiate although they were still producing both the butyrolactone-type autoregulator 1 and nadp-glycohydrolase, and b) mutants whose incapability to form spores and anthracycline pigments was apparently caused by the loss of autoregulator production. these latter mutants respond ... | 1984 | 6438925 |
| the effect of bacterial peptidoglycans on the immune response of hamsters to influenza virus vaccines. | the immune response of hamsters to influenza virus vaccine incorporating peptidoglycans derived from streptomyces griseus was investigated. the results showed that although inoculation of a subunit influenza virus vaccine together with a peptidoglycan elicited a markedly increased serum hi antibody response compared to that induced by the subunit vaccine alone, pre-treatment of animals with peptidoglycans resulted in a suppression of the antibody response to the vaccine haemagglutinin antigen. t ... | 1982 | 6811173 |
| [leukemomycin-blocked mutants of streptomyces griseus and their pigments. ii. new 7-hydroxy-bisnahydro-rhodomycinones from the mutant zimet 41707/1p]. | various blocked mutants were isolated from three leukaemomycin-(daunomycin-)producing strains imet ja 3933, imet ja 5142 and imet ja 5570 of streptomyces griseus by ntg and uv treatments. among them, one class of four mutants zimet 43707/1p, imet ja 5570/3p, imet ja 5570/10p and imet ja 5142/01p1 produced new blue and red pigments. two red compounds designated 1pi and 1pii are the main components of the pigment complex produced by culture of the blocked mutant zimet 43707/1p. this paper describe ... | 1984 | 6438926 |
| [detection of plasmid dna in different strains of streptomyces griseus]. | | 1982 | 6813654 |
| substructural study of sporogenesis in streptomyces griseus. | a streptomyces griseus strain deficient in the formation of aerial mycelium and arthrospore development (amy-) was studied by electron microscopy and compared with the sporulating parental strain (amy+). the investigations were performed with colonies grown on solid medium. the substructural characteristics of the essential events of sporogenesis could clearly been demonstrated in the aerial mycelium of the amy+ colonies. the mycelium of the surface region of the amy- colonies showed altered fea ... | 1984 | 6438927 |
| [chromosome mapping of streptomyces griseus 773 using interspecies recombination]. | | 1982 | 6815431 |
| pathways of phosphatidylethanolamine catabolism in streptomyces griseus. | | 1982 | 6816719 |
| nucleotide sequence of streptomyces griseus initiator trna. | the primary structure of initiator trna from streptomyces griseus was determined by post-labeling procedures. the nucleotide sequence is pc-g-c-g-g-g-g-u-g-g-a-g-c-a-g-c-u-c-g-g-d-a-g-c-u-c-g-c-u-g-g-g-c-u-c-a-u-a-a-c-c- c-a-g-a-g-g-u-c-g-c-a-g-g-u-psi-c-a-m1a-a-u-c-c-u-g-u-c-c-c-c-g-c-u-a-c-c-a0h. the unique feature of the sequence of this trna is that residue 54 is occupied by unmodified u, while ribothymidine is located in that position in most initiator trnas from eubacteria. | 1982 | 6817303 |
| crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with streptomyces griseus protease b. | | 1980 | 6777499 |
| structures of product and inhibitor complexes of streptomyces griseus protease a at 1.8 a resolution. a model for serine protease catalysis. | | 1980 | 6783761 |
| photoreactivating enzyme from streptomyces griseus--ii. evidence for the presence of an intrinsic chromophore. | | 1980 | 6784130 |
| photoreactivating enzyme from streptomyces griseus-iv. on the nature of the chromophoric cofactor in streptomyces griseus photoreactivating enzyme. | | 1981 | 6787622 |
| [canine fibrinogen glycopeptides]. | canine fibrinogen was digested by a complex of proteases from streptomyces griseus. the degradation products were purified by gel-filtration, deae-cellulose chromatography and electrophoresis, resulting in nine glycopeptides, eight of which contained aspartic acid and one--serine. the other amino acids were found only in trace amounts. the glycopeptides were shown to contain hexoamines, mannose, galactose and sialic acid. the oligosaccharide chains form a sequence of structurally similar variant ... | 1980 | 6788096 |
| [genetic recombination in mutants of the anthracycline-producer streptomyces griseus]. | it was found that genetic recombination occurs if two marked strains of streptomyces griseus (leukaemomycin-producing strains imet ja 3933 and imet ja 5142) are grown together in mixed cultures on semisolid media. the crossing techniques used and the method for carrying out selective analysis were essentially the same as those described by hopwood (1967, 1972). the parent strains used for crosses were marked with single or double nutritional requirements and with mutations for drug resistance. t ... | 1982 | 6819726 |
| c-19393 s2 and h2, new carbapenem antibiotics. i. taxonomy of the producing strain, fermentation and antibacterial properties. | c-19393 s2 and h2 are new carbapenem antibiotics produced by a streptomycete. the producing strain was taxonomically studied and named streptomyces griseus subsp. cryophilus. cobaltous compounds were necessary for production of the antibiotics. c-19393 s2 and h2 showed a broad spectrum of antibacterial activities with c-19393 h2 being 8 approximately 120 times more active than c-19393 s2. they also exhibited beta-lactamase-inhibiting activities and acted synergistically with ampicillin and cefot ... | 1980 | 6788739 |
| [properties of streptomyces griseus aminopeptidase]. | the paper deals with studying the properties of aminopeptidase isolated from str. griseus culture fluid. the preparation is characterized by a high specific activity and heat stability, it has no admixtures of carboxypeptidases and proteinases. the enzyme is easily inhibited by edta, but the addition of ca2+ evokes its complete reactivation. a partial recovery of the activity may be also reached under the influence of some other bivalent metals. in hydrolysis of di- and tripeptides it is shown t ... | 1980 | 6789520 |
| structure of the complex formed between the bacterial-produced inhibitor chymostatin and the serine enzyme streptomyces griseus protease a. | | 1980 | 6790710 |
| self-resistance of a streptomyces which produces istamycins. | streptomyces tenjimariensis ss-939, a producer of istamycins, is highly resistant to its own antibiotics and grows in tryptic soy broth containing istamycin a or b at 3,000 microgram/ml. no istamycin-inactivating enzyme was detected in extracts of strain ss-939. polyphenylalanine synthesis in an in vitro system, consisting of the s-150 fraction and ribosomes prepared from strain ss-939, was not inhibited by 200 microgram/ml of istamycins. using reciprocally reconstituted systems consisting of s- ... | 1981 | 7026523 |
| active site of alpha-lytic protease: enzyme-substrate interactions. | the active site of alpha-lytic protease has been studied with a number of synthetic peptides and compared with similar data published for elastase. the kinetic data indicate that the active site of alpha-lytic protease extends over at least six subsites (s4--s2'). this extended active site has the effect of increasing kcat/km by more than 10(6)-fold on going from an n-acetylated amino acid amide to a hexapeptide, due mainly to increases in kcat. there are major differences between alpha-lytic pr ... | 1981 | 7032913 |
| a sepharose derivative coupled with a leupeptin-like peptide aldehyde, glycylglycyl-l-argininal, and its use as an affinity adsorbent for trypsin. | a sepharose derivative containing a peptide aldehyde, glycylglycyl-l-argininal, the structure of which resembles that of leupeptin was prepared. it was a strong affinity adsorbent for trypsin (ec 3.4.21.4). bovine trypsin showed higher affinity for this adsorbent at the optimum ph of catalysis (8.2) than at lower ph (5.0). this observation was in good agreement with the ph dependence of the interaction of leupeptin and trypsin (kuramochi, h., nakata, h. and ishii, s. (1979) j. biochem. 86, 1403- ... | 1981 | 6793074 |
| evidence against a general role of nadp-glycohydrolase in differentiation of streptomyces griseus. | | 1981 | 6796557 |
| fredericamycin a, a new antitumor antibiotic. i. production, isolation and physicochemical properties. | a new antitumor antibiotic, fredericamycin a (fcrc-a48, nsc-305263), has been isolated from a strain of streptomyces griseus (fcrc-48). based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound. its production, isolation and physicochemical properties are discussed. the isolation, ultraviolet-visible spectrum and some biological properties of two mi ... | 1981 | 6798004 |
| [leukaemomycin-blocked mutants of streptomyces griseus and their pigments]. | in a continued search for leukaemomycin-blocked mutants of three leukaemomycin-producing strains imet ja 3933, imet ja 5142 and imet ja 5570 of streptomyces griseus, 32 mutants producing aerial mycelium and spores were detected. furthermore, in all mutants cosynthetic capability has been observed. this report describes characterization of leukaemomycin-blocked mutants obtained by mutagenic treatment experiments using ntg and combined uv-/x-rays. according to the biosynthetic capability for anthr ... | 1981 | 6801872 |
| trypsin inhibitor of streptomyces griseus grown in a carbon nutrition medium. | streptomyces griseus grown in beef extractpeptone medium produces trypsin inhibitors which are both peptide and protein in nature. when the strain was grown in carbon nutrition medium, only one trypsin inhibitor was obtained. similar elution patterns of this inhibitor and the ti-3a inhibitor obtained from the beef extract-peptone medium on sephadex g-15 and ion-exchange columns and also the rf values indicate that the inhibitors from both the sources are the same. so, ti-3a is not the breakdown ... | 1982 | 7173749 |
| a substance effecting differentiation in streptomyces griseus. purification and properties. | a conidium-producing variant of streptomyces griseus, strain 45-h, produces a substance, factor c, which is capable of inducing conidium formation in the hyphae of a conidium-non-producing mutant, strain 52-1. factor c can be determined quantitatively on the basis of this biological effect. the biologically active substance can be purified by ion-exchange chromatography on cellulose phosphate combined with affinity chromatography on dna-agarose. the purified substance is concentrated at least 17 ... | 1980 | 6767606 |
| the microbiology of spent mushroom compost and its dust. | microorganisms in spent steamed mushroom compost and its dust were enumerated, and identified. some phase ii (indoor composting) compost samples were also examined. steaming of spent compost resulted in a 70-76% reduction in microbial numbers. total counts made with compost fusion agar were approximately two logs greater than those for nutrient agar. the most common bacterial isolate was bacillus licheniformis. the most common actinomycete isolates were streptomyces diastaticus and thermoactinom ... | 1981 | 7197578 |
| streptomyces setonii: catabolism of vanillic acid via guaiacol and catechol. | streptomyces setonii (strain 75vi2) was grown at 45 degrees c in liquid media containing simple aromatic compounds as principal carbon sources. thin-layer chromatography, uv spectrophotometry, and gas chromatography were used to show that s. setonii converted benzoic acid, guaiacol, and vanillic acid to catechol; p-hydroxybenzoic acid to protocatechuic acid; and m-hydroxybenzoic acid to gentisic acid. presence of the ring-cleavage enzymes catechol 1,2-dioxygenase, protocatechuate 3,4-dioxygenase ... | 1981 | 7260738 |
| biosynthesis of streptomycin. purification and properties of a dtdp-l-dihydrostreptose: streptidine-6-phosphate dihydrostreptosyltransferase from streptomyces griseus. | dtdp-l-dihydrostreptose: streptidine-6-phosphate dihydrostreptosyltransferase, an enzyme involved in the biosynthesis of streptomycin, has been purified from streptomyces griseus to near homogeneity by a six-step procedure involving chromatography on streptidine-6-phosphate-sepharose. by gel filtration the apparent mr of the enzyme was found to be about 63 000. the subunit mr found on sodium dodecylsulfate gels is about 35 000. the transferase is dependent on mn2+ or mg2+ ions. co2+ is as effect ... | 1980 | 6768553 |
| active centers of alpha-chymotrypsin and of streptomyces griseus proteases 1 and 3. s2-p2 enzyme-substrate interactions. | a number of peptides of the general formula ac-pro-ala-x-phe-nh2, where x = gly, ala, leu, phe or pro, have been synthesized for the study of s2-p2 enzyme-substrate interactions in three serine proteases: alpha-chymotrypsin and streptomyces griseus proteases 1 and 3. all three enzymes interacted favorably with those peptides with hydrophobic, non-aromatic, p2 amino acid residues, leucine being optimal. the increase in kcat/km on going from the p2 glycine to the p2 leucine peptide was 30-fold in ... | 1980 | 6768556 |
| ionizable p1 residues in serine proteinase inhibitors undergo large pk shifts on complex formation. | the burial of charged residues in proteins is rare as it is thermodynamically strongly disfavored. however, in "standard mechanism" protein inhibitors of serine proteinases, the p1 residue, which is highly exposed, becomes buried in the s1 specificity pocket of the enzyme. in many enzymes, such as streptomyces griseus proteinase b (sgpb) the s1 pocket is hydrophobic. we measured the ph dependence of the association equilibrium constant for the interaction of sgpb with turkey ovomucoid third doma ... | 1995 | 7499197 |
| transglycosylation reaction of maltotriose-forming amylase from streptomyces griseus. | a maltotriose-forming amylase from streptomyces griseus produced predominantly p-nitrophenyl alpha-maltotetraoside through a transglycosylation reaction from maltotetraose as a donor and p-nitrophenyl alpha-d-glucopyranoside as an acceptor in an organic co-solvent. with p-nitrophenyl beta-d-glucopyranoside acceptor, the enzyme catalyzed the formation of an alpha-(1-->3)-linked tetrasaccharide (p-nitrophenyl 3(1)-o-alpha-maltotriosyl-beta-d-glucopyranoside) in a yield of 16%, based on the accepto ... | 1993 | 7511484 |
| giant linear plasmids of beta-lactam antibiotic producing streptomyces. | a survey of the total cellular dna from five beta-lactam antibiotic-producing streptomyces spp. by pulsed field gel electrophoresis was conducted to investigate the presence of linear plasmids. streptomyces clavuligerus nrrl 3585 contained two giant linear plasmids of 120 and 430 kb, in addition to the well-characterized 11.7 kb linear plasmid. streptomyces griseus nrrl 3851 contained a single giant linear plasmid of 120 kb, and streptomyces jumonjinensis nrrl 5741 contained two giant linear pla ... | 1995 | 7557307 |
| characterization of the cmch genes of nocardia lactamdurans and streptomyces clavuligerus encoding a functional 3'-hydroxymethylcephem o-carbamoyltransferase for cephamycin biosynthesis. | sequencing of orf10 (gene cmch) of the nocardia lactamdurans cephamycin gene cluster proved that it encodes a protein with a deduced molecular mass of 57,149 da. this protein showed significant similarity to the putative o-carbamoyltransferases (o-cases) encoded by the nodu genes of rhizobium fredii and bradyrhizobium japonicum, involved in the synthesis of nodulation factors. the carbamoyl-phosphate (cp)-binding amino-acid sequence of human otcase is conserved in the cmch product. a similar cmc ... | 1995 | 7557411 |
| interaction between aspergilli and streptomycetes in the soil of potted indoor plants: a preliminary report (contribution to the epidemiology of human aspergillosis). | the soil of potted ornamental plants as a reservoir for aspergillus species pathogenic for man is of epidemiological and ecological interest. isolation of a. niger as the sole hyphomycete from the soil of potted african violets (saintpaulia ionantha, gesneriaceae), prompted us to look for a. inger on the surface of the roots of this plant. small pieces of the roots were inoculated in the nutrient-free agar-gel with and without antibiotics. on the antibiotic-free gel, a dense growth of streptomyc ... | 1980 | 6769046 |
| antimicrobial activities of indolocarbazole and bis-indole protein kinase c inhibitors. ii. substitution on maleimide nitrogen with functional groups bearing a labile hydrogen. | new compounds, structurally related to the potent protein kinase c inhibitor staurosporine, and substituted on the imide nitrogen with a functional group bearing a labile hydrogen (hydroxymethyl, amino, hydroxy), were synthesized. their in vitro inhibitory potencies towards protein kinase c and protein kinase a showed that n-hydroxymethyl and n-hydroxy substitution, unlike alkyl substitution, can provide efficient protein kinase c inhibitors. the antimicrobial activities of these new compounds a ... | 1995 | 7592032 |