| human parotid size polymorphism (ps): characterization of two allelic products, ps 1 and 2, by limited proteolysis. | the allelically determined human salivary proteins, ps 1 and 2, were purified on sodium dodecyl sulfate gels, eluted, and compared by limited proteolysis with streptomyces griseus protease vi, bacillus subtilis protease vii, and staphylococcus aureus protease v8. prior dansylation of the ps isoproteins facilitated visualization of the peptides. digestion patterns indicate considerable homology between the ps isoproteins and support the conclusion [azen, a. e., and denniston, c. (1980). biochem. ... | 1983 | 6344862 |
| rapid diagnosis of group a streptococcal antigen extracted directly from swabs by an enzymatic procedure and used to detect pharyngitis. | coagglutination after enzymatic digestion is a widely used, rapid procedure for serogrouping isolated colonies of beta-hemolytic streptococci. we tried to determine whether the same procedure could be used for the detection of group a streptococcal antigen directly from swabs used to take throat samples. this was achieved by incubating the swabs immersed in a small quantity of lytic extract obtained from cultures of the maxted strain of streptomyces griseus and testing the supernatant fluid by c ... | 1983 | 6352733 |
| [extracellular serine proteinase a of streptomyces rutgersensis]. | extracellular serine proteinase of streptomyces rutgersensis (proteinase a) has been isolated by affinity chromatography on bacitracin-sepharose in the presence of na2edta. the proteolytic activity of the enzyme is completely suppressed by specific inhibitors of serine proteinases, phenylmethylsulfonylfluoride and diisopropylfluorophosphate, but is insensitive to na2edta and sodium p-hydroxymercuribenzoate. the enzyme activity towards hemoglobin is maximal at ph 9-10, the maximal stability is ob ... | 1983 | 6354278 |
| phospholipase b from the plasma membrane of saccharomyces cerevisiae. separation of two forms with different carbohydrate content. | two forms of phospholipase b could be solubilized from the plasma membrane of saccharomyces cerevisiae, separated by gel filtration with sephacryl s-300 and identified by sds-polyacrylamide gel electrophoresis as glycoproteins of the apparent molecular weights of about 220 000 (phospholipase b1) and 145 000 (phospholipase b2). the enzymes are very similar in respect to their catalytic properties. both forms converted lysophosphatidylcholine to diacylphosphatidylcholine and unesterified fatty aci ... | 1984 | 6380591 |
| phosphoenolpyruvate-dependent phosphorylation site in enzyme iiiglc of the escherichia coli phosphotransferase system. | enzyme-iiiglc is part of the glucose phosphotransferase system of escherichia coli and salmonella typhimurium and is phosphorylated by phosphoenolpyruvate in a reaction requiring enzyme i (phosphoenolpyruvate-protein phosphotransferase), and the histidine-containing phospho-carrier protein hpr. in this paper we report the isolation of iiiglc from e. coli and the characterization of the active center. alkaline hydrolysis of [32p]p-iiiglc and chromatography of the hydrolysate suggested that the ph ... | 1984 | 6383826 |
| [protoplasts of streptomyces griseus and its auxotrophic derivatives]. | | 1984 | 6400803 |
| [growth and antimicrobial activity of an association of an actinomycete and a green alga]. | during joint cultivation of the actinomycetes streptomyces griseus (strains 65 and 744) isolated from the soil and the green algae chlorella vulgaris larger amount of biomass as compared with solitary axenic culture have been shown. the relation of biomass of actinomycetes s. griseus strain 65 and s. griseus strain 744 and algae in the lichen-like experimentally formed thallom make up 42:1 and 40:1 relatively, i. e. the mass of actinomycetes forms 97-98% from the mass of thalloms. actinomycetes ... | 1983 | 6405815 |
| [effect of preservation conditions on the viability and activity of streptomyces griseus--producer of the antibiotic grizin]. | strain aurigenetics- 1552 of str. griseus producing grisin used as a feed additive was obtained in the course of stepped selection. it is deposited at the central collection of industrial microorganisms of the ussr and designated as strain s-248. the strain was stored under varying conditions and their effect on its viability, morphological variation and antibiotic potency was studied. investigation of the strain variation with respect to the cultural and morphological properties and the antibio ... | 1983 | 6407387 |
| [isolation and regeneration of streptomyces griseus protoplasts]. | | 1983 | 6408362 |
| [effect of the a-factor on the adenylate level in streptomyces griseus]. | the adenylate content of various strains of streptomyces griseus was measured. with respect to the atp and adp content the strains capable of spore formation, streptomycin synthesis and a-factor (2-isocapryloyl-3-hydroxymethyl-4-hydroxybutyrolactone) synthesis differed from the a-factor deficient mutants. the addition of the a-factor to the recipient strains decreased the intracellular content of atp and the atp/adp ratio. the strain which is not an a-factor recipient did not modify the atp cont ... | 1983 | 6410372 |
| inhibition of metalloendopeptidases by 2-mercaptoacetyl-dipeptides. | a series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the n-hydroxysuccinimide ester of s-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with naoh in aqueous methanol and acidification with hcl. thus, the 2-mercaptoacetyl derivatives of l-phenylalanyl-l-leucine, l-leucyl-l-phenylalanine and l-leucyl-d-phenylalanine were prepared. the first two compounds inhibit e ... | 1983 | 6413206 |
| structure of the complex of streptomyces griseus protease b and the third domain of the turkey ovomucoid inhibitor at 1.8-a resolution. | the structure of the complex between the serine protease streptomyces griseus protease b (sgpb) and the third domain of the kazal-type ovomucoid inhibitor from turkey has been solved at 1.8-a resolution and refined to a conventional r factor of 0.125. as others have reported previously for analogous complexes of proteases and protein inhibitors, the inhibitor binds in a fashion similar to that of a substrate; it is not cleaved, but there is a close approach (2.7 a) of the active site nucleophile ... | 1983 | 6414511 |
| biochemical investigations of streptomycin production by streptomyces griseus. | | 1983 | 6414994 |
| genetic analysis of a-factor synthesis in streptomyces coelicolor a3(2) and streptomyces griseus. | a-factor is a potent pleiotropic effector produced by streptomyces griseus and is essential for streptomycin production and spore formation in this organism. its production is widely distributed among various actinomycetes including streptomyces coelicolor a3(2). genetic analysis of a-factor production was carried out with s. coelicolor a3(2), and two closely linked loci for a-factor mutations (afsa and b) were identified between cysd and leub on the chromosomal linkage map. in contrast, genetic ... | 1983 | 6415235 |
| relation of anabolic-catabolic glucose utilization in growth-limited cultures of streptomyces griseus. | phenotypically different submerged mycelium conserves had been produced from a spore conserve of the hp-strain streptomyces griseus and proofed in a product formation culture as a test system. the phenotypical characters induced on the base of the genotype proved in a cultivation cycle during 30-34 reduplications of the biomass constant. employing the hp phenotype we investigated the possibility of economizing the substrate turnover by utilizing the anabolic potential for the synthesis of second ... | 1983 | 6415934 |
| effect of the autoregulator from streptomyces griseus ja 5142 on surface cultures of blocked mutant zimet 43682. | zero time addition of the autoregulator (l-factor) from s. griseus (lkm+amy+) to surface cultures of its bald mutant zimet 43 682 (amy-lkm-) restored the capacity to form both anthracycline-type antibiotic leukaemomycin and aerial mycelium. the pertinent mycelia displayed the same growth rate and cellular levels of nucleic acids as the asporogeneous phenotype but the composition of fatty acids and phospholipids as well as the ratio of cytochromes b and c were altered. these differences indicate ... | 1983 | 6415935 |
| [use of the protoplast fusion method in the selection of streptomyces griseus--the producer of the streptothricin antibiotic grisin]. | an effective method for protoplast fusion in s. griseus producing grisin was developed. the method requires the use of polyethylene glycol with a molecular weight of 1000. it was demonstrated that protoplasts formed most effectively in this organism, when the mycelium of the streptomycete previously treated with ultrasound in the process of its growth was used for the treatment with lysozyme. the efficacy of protoplast regeneration in the strains with the use of the modified hypertonic medium r2 ... | 1983 | 6419670 |
| cloning and expression of a p-aminobenzoic acid synthetase gene of the candicidin-producing streptomyces griseus. | 4.5-kb bamhi fragments of dna coding for p-aminobenzoic acid (paba) synthetase from the candicidin-producing streptomyces griseus imru 3570 and from a sulphonamide resistant mutant of it were cloned on the plasmid vector pij41 into streptomyces lividans 66. the cloned dna restored prototrophy to a pab auxotroph of s. lividans; when the s. griseus donor was a sulphonamide resistant, paba-overproducing mutant, the s. lividans clone was sulphonamide resistant as well as pab+. sub-cloning the 4.5-kb ... | 1983 | 6420235 |
| isolation and characterization of pock-forming plasmids for streptomyces griseus from soil actinomycetes. | thirty independent actinomycetes strains carrying plasmids were isolated from soil. these plasmids were purified as cccdna by cscl-etbr equilibrium density-gradient centrifugation. plasmids that induce "pocks", namely formation of circular zones of sporulation-inhibition, were selected by protoplast transformation of streptomycin-producing strain, streptomyces griseus atcc10137. six pock-forming plasmids, poa7, poa11, poa15, poa23, poa29 and poa30, were obtained, and their cleavage maps, transfo ... | 1983 | 6420237 |
| studies on ti-2 trypsin inhibitor of streptomyces griseus. | the trypsin inhibitor ti-2 produced by streptomyces griseus cal in a medium, containing beef extract and peptone as the nitrogen source, was purified by ammonium sulfate precipitation and gel filtration. amino acid analysis showed that it contained hydroxyproline and carbohydrate whereas tyrosine, tryptophan, and cystine were absent. the composition of the inhibitor protein thus showed a similarity to collagen. growth of the organism in a medium with gelatin as the sole nitrogen source resulted ... | 1983 | 6421469 |
| metabolic products of microorganisms. 224. bafilomycins, a new group of macrolide antibiotics. production, isolation, chemical structure and biological activity. | the bafilomycins a1, a2, b1, b2, c1 and c2, a new type of macrolide antibiotics with a 16-membered lactone ring, were isolated from the fermentation broth of three streptomyces griseus strains (tu 1922, tu 2437, tu 2599) by ethyl acetate extraction and column chromatography on silica gel. the bafilomycins exhibit activity against gram-positive bacteria and fungi. physico-chemical data, chemical structures and biological activities are reported. | 1984 | 6423597 |
| [carboxypeptidase t--intracellular carboxypeptidase of thermoactinomycetes--a distant analog of animal carboxypeptidase]. | carboxypeptidase t, an extracellular carboxypeptidase from thermoactinomyces sp. was isolated and purified by affinity chromatography on bacitracin adsorbents. the enzyme homogeneity was established by sds electrophoresis (mr = 38 000) and isoelectrofocusing in paag (pi 5.3). carboxypeptidase t reveals a mixed specificity in comparison with pancreatic carboxypeptidases a and b and cleaves with nearly the same efficiency the peptide bonds formed by the c-terminal residues of basic and neutral hyd ... | 1984 | 6424730 |
| inducers of both cytodifferentiation and anthracycline biosynthesis of streptomyces griseus and their occurrence in actinomycetes and other microorganisms. | many taxonomically different strains of actinomycetes as well as several other procaryotic and eucaryotic microorganisms were shown to produce inducers of cytodifferentiation and anthracycline biosynthesis being active towards blocked mutant zimet 43682 of streptomyces griseus. 37 out of 40 strains of the species of s. griseus (92.5%) yielding different kinds of antibiotics were able to induce both the formation of aerial mycelium in the indicator strain zimet 43682 concomitant with the biosynth ... | 1984 | 6426178 |
| unstable genetic determinant of a-factor biosynthesis in streptomycin-producing organisms: cloning and characterization. | we cloned a dna fragment directing synthesis of a-factor from the total cellular dna of streptomycin-producing streptomyces bikiniensis on the plasmid vector pij385 . introduction of the recombinant plasmid ( pafb1 ) into a-factor-deficient s. bikiniensis and streptomyces griseus mutants led to a-factor production in the host cells, as a result of which streptomycin production, streptomycin resistance, and spore formation of these mutants were simultaneously restored. the plasmid pafb1 also comp ... | 1984 | 6427181 |
| protein patterns of streptomyces griseus strains during development. | protein patterns from mycelial extracts of streptomyces griseus strains no. 45h and no. 52-1 were studied by one dimensional gradient page with 20 cm run. the results are reproducible, the protein patterns are the same for the same developmental stage in a given strain. there are well-defined characteristic changes of the protein patterns during the life cycle. the proteins of spores show conspicuous differences compared to protein patterns of the old (72 h) mycelia in the same culture. there is ... | 1984 | 6428069 |
| pleiotropic effects of a butyrolactone-type autoregulator on mutants of streptomyces griseus blocked in cytodifferentiation. | mutants of streptomyces griseus blocked in cytodifferentiation regained their capacity to form differentiated mycelia and/or anthracycline pigments in the presence of butyrolactone-type autoregulatory effectors such as trans-2-(6'-methylheptanol-1'-yl)-3-hydroxymethyl-4-butanolide+ ++. in the pertinent indicator strains, the effect has been correlated with the increase of lipid synthesis, with changes in the composition of lipid fraction and with the restoration of the production of neutral prot ... | 1984 | 6432945 |
| proline transport increases growth efficiency in salt-stressed streptomyces griseus. | streptomyces griseus synthesizes proline for osmoregulation under salt stress. uptake of exogenous [14c]proline and internal synthesis of proline were quantified in cells growing at salt concentrations from 0 to 1 m nacl. externally supplied proline accounted for an increased proportion of the intracellular pool of free proline as salt concentration was increased, but neither the concentration nor the composition of the internal amino acid pool was substantially altered by supply of exogenous pr ... | 1984 | 6433794 |
| structure of the oligosaccharides sensitive to endo-beta-n-acetylglucosaminidase h in the glycoprotein of friend murine leukemia virus. | the surface glycoprotein (mixture of isoglycoproteins with mr 69 000 and 71 000) was isolated from the particles of friend murine leukemia virus, and was successively digested with protease and with endo-beta-n-acetylglucosaminidase from streptomyces griseus. roughly 20% (w/w) of the carbohydrates in this glycoprotein were thus released, and they were fractionated by high-performance liquid chromatography after reduction with kb3h4/nabh4. the radioactive oligosaccharitol fractions obtained were ... | 1984 | 6434306 |
| griseochelin, a novel carboxylic acid antibiotic from streptomyces griseus. | griseochelin, c33h60o7, isolated from an asporogenous strain of streptomyces griseus represents a novel carboxylic acid antibiotic. the metabolite, which is active against gram-positive bacteria, forms water-insoluble salts with mono- and divalent cations and binds alkaline-earth metal ions specifically in 2:1 (x2m) stoichiometry. detailed spectral (ir, ms and nmr) studies provide full characterization of its constitution featuring a carboxylic acid function, a substituted tetrahydropyran ring, ... | 1984 | 6434502 |
| effect of trypsin inhibitors of streptomyces griseus cal. on blood clotting and fibrinolysis. | | 1984 | 6436171 |
| modification by genetic changes of the pleiotropic interference of butyrolactone-type autoregulators with differentiation of streptomyces griseus. | two series of aerial-mycelium-negative (amy-), anthracycline-nonproducing (ant-) mutants were obtained from ancestral amy+ant+ strains of s. griseus: a) derivatives represented by the met- strain 39 which could not differentiate although they were still producing both the butyrolactone-type autoregulator 1 and nadp-glycohydrolase, and b) mutants whose incapability to form spores and anthracycline pigments was apparently caused by the loss of autoregulator production. these latter mutants respond ... | 1984 | 6438925 |
| [leukemomycin-blocked mutants of streptomyces griseus and their pigments. ii. new 7-hydroxy-bisnahydro-rhodomycinones from the mutant zimet 41707/1p]. | various blocked mutants were isolated from three leukaemomycin-(daunomycin-)producing strains imet ja 3933, imet ja 5142 and imet ja 5570 of streptomyces griseus by ntg and uv treatments. among them, one class of four mutants zimet 43707/1p, imet ja 5570/3p, imet ja 5570/10p and imet ja 5142/01p1 produced new blue and red pigments. two red compounds designated 1pi and 1pii are the main components of the pigment complex produced by culture of the blocked mutant zimet 43707/1p. this paper describe ... | 1984 | 6438926 |
| substructural study of sporogenesis in streptomyces griseus. | a streptomyces griseus strain deficient in the formation of aerial mycelium and arthrospore development (amy-) was studied by electron microscopy and compared with the sporulating parental strain (amy+). the investigations were performed with colonies grown on solid medium. the substructural characteristics of the essential events of sporogenesis could clearly been demonstrated in the aerial mycelium of the amy+ colonies. the mycelium of the surface region of the amy- colonies showed altered fea ... | 1984 | 6438927 |
| comparison of protein patterns of streptomyces griseus spores from surface and submerged cultures. | protein patterns of conidia produced by a streptomyces griseus strain in submerged cultures and on solid media were compared. cell-free extracts (30 000 x g supernatant) were prepared and analyzed on gradient sds polyacrylamide gels. the protein patterns of both kinds of conidia were found to be practically identical, and they differed from protein patterns of the old vegetative hyphae in a characteristic way. | 1984 | 6441361 |
| critical evaluation of comparative model building of streptomyces griseus trypsin. | the x-ray crystal structure of streptomyces griseus trypsin has been solved and refined at 1.7-a resolution. the structure of this protein had been predicted in two models on the basis of its expected homology to structures of bovine trypsin and other pancreatic serine proteases [jurásek, l., olafson, r.w., johnson, p., & smillie, l.b. (1976) miami winter symp. 11, 93-123; greer, j. (1981) j. mol. biol. 153, 1027-1042]. an evaluation of these models in light of the known structure demonstrates t ... | 1984 | 6442164 |
| [regulators of differentiation in streptomyces cyaneofuscatus]. | streptomyces cyaneofuscatus prl 1642 producing valinomycin was shown to synthesize bioregulators of differentiation similar to a factor. the regulators stimulate spore formation and streptomycin synthesis in a streptomyces griseus 1439 mutant deficient in a factor. some s. cyaneofuscatus mutants respond to a factor addition into the medium by an increase in valinomycin synthesis and a change in morphological properties. the regulators from s. cyaneofuscatus are more effective toward mutants of t ... | 1984 | 6442388 |
| examination of protein sequence homologies: i. eleven escherichia coli l7/l12-type ribosomal "a" protein sequences from eubacteria and chloroplast. | seven complete and four partial sequences of escherichia coli l7/l12-type ribosomal "a" proteins obtained from various bacteria (e. coli, bacillus subtilis, micrococcus lysodeikticus, rhodopseudomonas spheroides, desulfovibrio vulgaris, streptomyces griseus, bacillus stearothermophilus, clostridium pasteurianum, arthrobacter glacialis, and vibrio costicola) and spinach chloroplast have been reexamined using a computer program that searches for homologous tertiary structures. comparison matrices ... | 1984 | 6443314 |
| biosynthetic studies of amphotericins, candicidin and nystatin by means of mutation. | a novel mutant strain designated as streptomyces nodosus, ndmc-034 was selected from the mutation of streptomyces nodosus, imru 3694. this mutant was characterized by delayed sporulation, the incapability of producing the melanoid pigment either in the production medium or on yd agar containing 1% yeast extract and 1% dextrose, and the increased yield of amphotericin a as compared to its parent strain. in addition, the biosynthesis of amphotericin a by this mutant seems to be sensitive to temper ... | 1984 | 6443786 |
| [isolation of streptomyces griseus mutants by blocking streptomycin synthesis and research on their proteolytic activity]. | | 1983 | 6443858 |
| [genetic recombination and chromosome mapping of streptomyces griseus 773]. | | 1983 | 6443859 |
| [comparison of different methods for the identification of groups of streptococci]. | the streptococci, isolated from 500 mucus-pharyngeal tampons, have been tested, for a group identification, by means of four different techniques in order to value the specificity and reliability in comparison with more traditional and, sometimes, more complex tests; such as maxted and lancefield. the most suitable method for routine researchers of microbiology laboratories is the one based on the extraction, by means of enzyme obtained from streptomyces griseus, of streptococci antigens before ... | 1983 | 6626338 |
| stimulation of rat peritoneal mast cell migration by tumor-derived peptides. | the accumulation of mast cells is characteristic of a number of pathological states. we demonstrate here the directional motility of mast cells in vitro in response to tumor-derived peptides. rat peritoneal mast cells were isolated on percoll gradients and maintained in serum-free medium containing transferrin, albumin, soybean lipid, and cholesterol. the isolated mast cells migrated under agarose in response to medium conditioned by any of eight tumor cell lines but not to medium conditioned by ... | 1983 | 6640537 |
| metabolism of cinnamic, p-coumaric, and ferulic acids by streptomyces setonii. | streptomyces setonii strain 75vi2 was grown at 45 degrees c in liquid media containing yeast extract and trans-cinnamic acid, p-coumaric acid, ferulic acid, or vanillin. gas chromatography, thin-layer chromatography, and mass spectrometry showed that cinnamic acid was catabolized via benzaldehyde, benzoic acid, and catechol; p-coumaric acid was catabolized via p-hydroxybenzaldehyde, p-hydroxybenzoic acid, and protocatechuic acid; ferulic acid was catabolized via vanillin, vanillic acid, and prot ... | 1983 | 6661696 |
| a new polyene with antimycotic activity produced by a streptomycete from the rhizosphere of an indoor plant. | from the rhizosphere of a potted sanseviera trifasciata, a streptomyces griseus strain with a broad antifungal activity was isolated; in the soil of this plant, a sole hyphomycete, aspergillus niger, was found in pure culture at 37 degrees c. this streptomycete produced a polyene, belonging to the pentaene group. it was found to inhibit, in vitro, the growth of all the 123 strains tested; the strains belonging to the genera aspergillus, cryptococcus, candida, trichophyton, epidermophyton, micros ... | 1983 | 6681974 |
| characterization of antigens on mosquito midgut stages of plasmodium gallinaceum. i. zygote surface antigens. | we have defined the surface protein antigens on plasmodium gallinaceum zygotes using radioiodination methods and rabbit anti-zygote serum which blocks transmission of the parasite to aedes aegypti mosquitoes. fifteen protein bands (1-15) in the molecular weight range of 40 000-240 000 and one band at the bromophenol blue dye marker were labelled by the lactoperoxidase and iodogen (1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenylglycoluril) methods. the localization of these radioiodinated components ... | 1983 | 6683783 |
| structure of the major oligosaccharides in the fusion glycoprotein of newcastle disease virus. | the fusion glycoprotein (f0) was isolated from newcastle disease virus (ndv) particles metabolically labelled with [2-3h]mannose; it was successively digested with protease and with endo-beta-n-acetylglucosaminidase from streptomyces griseus. in this manner, the majority of the oligosaccharides in ndv f0 could be liberated. after reduction with nabh4, they were separated by high-performance liquid chromatography, and were subjected to structural analysis. using micromethylation/capillary gas chr ... | 1984 | 6698016 |
| secondary structure and phylogeny of staphylococcus and micrococcus 5s rrnas. | nucleotide sequences of 5s rrnas from four bacteria, staphylococcus aureus smith (diffuse), staphylococcus epidermidis atcc 14990, micrococcus luteus atcc 9341 and micrococcus luteus atcc 4698, were determined. the secondary structural models of s. aureus and s. epidermidis sequences showed characteristics of the gram-positive bacterial 5s rrna (116-n type [h. hori and s. osawa, proc. natl. acad. sci. u.s.a. 76:381-385, 1979]). those of m. luteus atcc 9341 and m. luteus atcc 4698 together with t ... | 1984 | 6735981 |
| formation of a reactive iminium derivative by enzymatic and chemical oxidations of 16-o-acetylvindoline. | 16-o- acetylvindoline (1a) was oxidatively transformed into an iminium derivative (2a) by copper oxidases (laccase and human ceruloplasmin), an unknown enzyme system(s) of streptomyces griseus, and the chemical oxidizing agent 2,3-dichloro-5,6- dicyano -1,4-benzoquinone ( ddq ). the iminium derivative (2a) was isolated from enzymatic and chemical oxidation mixtures and was identified by spectral and chemical techniques. reduction of the iminium compound with sodium borodeuteride provided monodeu ... | 1984 | 6737416 |
| refined crystal structure of the molecular complex of streptomyces griseus protease b, a serine protease, with the third domain of the ovomucoid inhibitor from turkey. | we have determined the crystal structure of the molecular complex between streptomyces griseus protease b (sgpb), a bacterial serine protease, and the third domain of the ovomucoid inhibitor from turkey. restrained-parameter least-squares refinement of the structure with the 1.8-a intensity data set has resulted in an r factor of 0.125. the carbonyl carbon atom of the reactive bond between leu-18 and glu-19 in the inhibitor lies at a distance of 2.71 a from the o gamma atom of the nucleophilic s ... | 1982 | 6750612 |
| complete amino acid sequence of streptokinase and its homology with serine proteases. | the complete amino acid sequence of streptokinase has been determined by automated edman degradation of its cyanogen bromide and proteolytic fragments. the protein consists of 415 amino acid residues. sequence microheterogeneity was found at two positions. the nh2-terminal 245 residues of streptokinase are homologous to the sequences of several serine proteases including bovine trypsin and streptomyces griseus proteases a and b. the sequence alignment suggests that the active-site histidine-57 h ... | 1982 | 6760891 |
| studies on prekallikrein of bovine plasma. ii. activation of prekallikrein with proteinases and properties of kallikrein activated by bovine hageman factor. | activation of bovine plasma prekallikrein was investigated with several proteinases. highly purified bovine plasma prekallikrein was rapidly activated to kallikrein [ec 3.4.21.8] by bovine activated hageman factor, trypsin [ec 3.4.21.4] and pronase p (proteinases from streptomyces griseus) and more gradually by papain [ec 3.4.22.2] and ficin [ec 3.4.22.3]. activation of prekallikrein was also observed with bovine plasmin [ec 3.4.21.7], but not with bovine clotting factors xa (stuart factor) [ec ... | 1980 | 6766924 |
| a substance effecting differentiation in streptomyces griseus. purification and properties. | a conidium-producing variant of streptomyces griseus, strain 45-h, produces a substance, factor c, which is capable of inducing conidium formation in the hyphae of a conidium-non-producing mutant, strain 52-1. factor c can be determined quantitatively on the basis of this biological effect. the biologically active substance can be purified by ion-exchange chromatography on cellulose phosphate combined with affinity chromatography on dna-agarose. the purified substance is concentrated at least 17 ... | 1980 | 6767606 |
| biosynthesis of streptomycin. purification and properties of a dtdp-l-dihydrostreptose: streptidine-6-phosphate dihydrostreptosyltransferase from streptomyces griseus. | dtdp-l-dihydrostreptose: streptidine-6-phosphate dihydrostreptosyltransferase, an enzyme involved in the biosynthesis of streptomycin, has been purified from streptomyces griseus to near homogeneity by a six-step procedure involving chromatography on streptidine-6-phosphate-sepharose. by gel filtration the apparent mr of the enzyme was found to be about 63 000. the subunit mr found on sodium dodecylsulfate gels is about 35 000. the transferase is dependent on mn2+ or mg2+ ions. co2+ is as effect ... | 1980 | 6768553 |
| active centers of alpha-chymotrypsin and of streptomyces griseus proteases 1 and 3. s2-p2 enzyme-substrate interactions. | a number of peptides of the general formula ac-pro-ala-x-phe-nh2, where x = gly, ala, leu, phe or pro, have been synthesized for the study of s2-p2 enzyme-substrate interactions in three serine proteases: alpha-chymotrypsin and streptomyces griseus proteases 1 and 3. all three enzymes interacted favorably with those peptides with hydrophobic, non-aromatic, p2 amino acid residues, leucine being optimal. the increase in kcat/km on going from the p2 glycine to the p2 leucine peptide was 30-fold in ... | 1980 | 6768556 |
| interaction between aspergilli and streptomycetes in the soil of potted indoor plants: a preliminary report (contribution to the epidemiology of human aspergillosis). | the soil of potted ornamental plants as a reservoir for aspergillus species pathogenic for man is of epidemiological and ecological interest. isolation of a. niger as the sole hyphomycete from the soil of potted african violets (saintpaulia ionantha, gesneriaceae), prompted us to look for a. inger on the surface of the roots of this plant. small pieces of the roots were inoculated in the nutrient-free agar-gel with and without antibiotics. on the antibiotic-free gel, a dense growth of streptomyc ... | 1980 | 6769046 |
| [changes in the actinophage sensitivity and resistance of a culture of actinomyces griseus and its variants]. | susceptibility and resistance of the culture of actinomyces griseus 15 and its variants to actinophages was studied. variants different from the parent culture in their susceptibility or resistance to certain phages can arise as the result of spontaneous variability and lysogenization. the culture of actinomyces griseus 15 resistant to nine polyphates was found to be able to adsorb them. the mycelium of act. griseus 15 was shown to contain receptors for a page to which it was susceptible differe ... | 1980 | 6771500 |
| partial purification, characterization and localization of the membrane-associated invertase of yeast. | the membrane-associated isozyme of invertase (beta-d-fructofuranoside fructo-hydrolase, ec 3.2.1.26) -- precursor of the external glycoprotein invertase (babczinski, p. and tanner, w. (1978) biochim. biophys. acta 538, 426-434) - has been purified 60-fold from deoxycholate extracts of derepressed yeast cells. the partially purified enzyme exhibits considerable stability as a salt-free lyophilized powder. its molecular weight, in this precursor form, has been determined by by sodium dodecyl sulph ... | 1980 | 6772225 |
| the effects of n-substitution of chitosan and the physical form of the products on the rate of hydrolysis by chitinase from streptomyces griseus. | n-formyl, n-chloroacetyl, n-glycyl, n-isobutyryl, and n-pentanoyl derivatives of chitosan have been prepared. n-acetylchitosan was the derivative most susceptible to chitinase from streptomyces griseus and lysozyme from chicken egg-white, but with respect to r in the rcohn group were ch3 > ch3ch2 > h > ch3ch2ch2 > (ch3)2ch > nh2ch2 > cich2. neither enzyme hydrolysed chitosan or its n-methylene. n-benzylidene, n-benzoyl, n-nicotinyl, and n-fatty acyl (c5-c18) derivatives, and lysozyme did not hyd ... | 1980 | 6773658 |
| regulation by aromatic amino acids of the biosynthesis of candicidin by streptomyces griseus. | the biosynthesis by streptomyces griseus of candicidin, an aromatic polyene macrolide antibiotic, was inhibited by l-tryptophan, l-phenylalanine and, to a lesser degree, by l-tyrosine. a mixture of the three aromatic amino acids inhibited candicidin biosynthesis to a greater extent than did each amino acid separately. l-tryptophan strongly inhibited the incorporation of the labelled precursors propionate or 4-aminobenzoic acid into candicidin. incorporation of propionate into candicidin was 50% ... | 1980 | 6775046 |
| anabolic-catabolic glucose utilization and product formation of streptomyces griseus. | if in stationary growth phases appearing in the submerged cultivation of streptomyces griseus several times the consumption rates of glucose and oxygen and the rates of streptomycin formation are put in relation, the following results are obtained: the yield coefficient ysp of glucose was below 0.1. since from the stoichiometric equation of turnover for the biosynthesis of the streptomycin follows that the substrate and the product are in the same weight relation, it was possible to check whethe ... | 1980 | 6775425 |
| field desorption mass spectrometry in structural studies of polyene macrolide antibiotics: isolation and early identification of a pentaene macrolide antibiotic. | isolation of a pentaene macrolide antibiotic (nsc-277813) from streptomyces griseus (fcrc-21) fermentation broth is described. using field desorption mass spectrometry and high resolution field desorption mass spectrometry on the intact and derivatized antibiotic and degradation products, the antibiotic was identified as fungichromin. the application of field desorption mass spectrometry in the identification of polyene antibiotics is discussed. | 1980 | 6775705 |
| crystal structure studies and inhibition kinetics of tripeptide chloromethyl ketone inhibitors with streptomyces griseus protease b. | | 1980 | 6777499 |
| plasmid deoxyribonucleic acid replication in streptomyces griseus. | a series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from streptomyces griseus was obtained. based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in s. griseus is proposed. | 1981 | 6783620 |
| structures of product and inhibitor complexes of streptomyces griseus protease a at 1.8 a resolution. a model for serine protease catalysis. | | 1980 | 6783761 |
| photoreactivating enzyme from streptomyces griseus--ii. evidence for the presence of an intrinsic chromophore. | | 1980 | 6784130 |
| control by phospho-adenosinediphospho-ribose of nadp-dependent isocitrate dehydrogenase and 6-phosphogluconate dehydrogenase in streptomyces griseus. | the metabolic function of nad(p)-glycohydrolase in the streptomycin-producing streptomyces griseus was investigated. phospho-adenosinediphospho-ribose, the product of nad(p)-glycohydrolase reaction was shown to interfere as a competitive inhibitor not only with the glucose-6-phosphate dehydrogenase (voronina et al. 1978) but also with the nadp-dependent isocitrate and 6-phospho-gluconate dehydrogenases. inhibition kinetics were studied with isocitrate dehydrogenase from pig heart and 6-phosphogl ... | 1980 | 6784352 |
| characterization of phospholipase a in streptomyces griseus. | | 1980 | 6785216 |
| [isolation of protease complexes from streptomyces griseus by ultrafiltration]. | the possibility of concentrating proteases from streptomyces griseus by the ultrafiltration technique using soviet membranes of the mb-i-iv type was explored. this method allowed a 3-10 fold reduction of the native solution of enzymes and their simultaneous purification from by-products. | 1981 | 6787580 |
| photoreactivating enzyme from streptomyces griseus-iv. on the nature of the chromophoric cofactor in streptomyces griseus photoreactivating enzyme. | | 1981 | 6787622 |
| microbial transformation of n-methylcolchiceinamide. | seventy-seven microorganisms were examined for their ability to metabolize the antineoplastic agent n-methylcolchiceinamide, an analog of colchicine. five streptomycetes exhibited significant metabolism, and streptomyces griseus nrrl b-599 completely converted the substrate to three metabolites. in preparative-scale studies, n-dealkylation resulted in the production of colchiceinamide, the major metabolite (65%), which was characterized by chemical, spectroscopic, and chromatographic comparisons ... | 1981 | 6787979 |
| [canine fibrinogen glycopeptides]. | canine fibrinogen was digested by a complex of proteases from streptomyces griseus. the degradation products were purified by gel-filtration, deae-cellulose chromatography and electrophoresis, resulting in nine glycopeptides, eight of which contained aspartic acid and one--serine. the other amino acids were found only in trace amounts. the glycopeptides were shown to contain hexoamines, mannose, galactose and sialic acid. the oligosaccharide chains form a sequence of structurally similar variant ... | 1980 | 6788096 |
| c-19393 s2 and h2, new carbapenem antibiotics. i. taxonomy of the producing strain, fermentation and antibacterial properties. | c-19393 s2 and h2 are new carbapenem antibiotics produced by a streptomycete. the producing strain was taxonomically studied and named streptomyces griseus subsp. cryophilus. cobaltous compounds were necessary for production of the antibiotics. c-19393 s2 and h2 showed a broad spectrum of antibacterial activities with c-19393 h2 being 8 approximately 120 times more active than c-19393 s2. they also exhibited beta-lactamase-inhibiting activities and acted synergistically with ampicillin and cefot ... | 1980 | 6788739 |
| c-19393 s2 and h2, new carbapenem antibiotics. ii. isolation and structures. | two new beta-lactam antibiotics, c-19393 s2 (1) and h2 (2), were isolated from the culture filtrate of streptomyces griseus subsp. cryophilus nov. subsp. the structures were determined by spectral analysis as shown in fig. 2. the antibiotics have broad antimicrobial activity and strongly inhibit beta-lactamases. the minor product (2) is more stable than cephalosporin c in aqueous solution. | 1980 | 6788740 |
| [properties of streptomyces griseus aminopeptidase]. | the paper deals with studying the properties of aminopeptidase isolated from str. griseus culture fluid. the preparation is characterized by a high specific activity and heat stability, it has no admixtures of carboxypeptidases and proteinases. the enzyme is easily inhibited by edta, but the addition of ca2+ evokes its complete reactivation. a partial recovery of the activity may be also reached under the influence of some other bivalent metals. in hydrolysis of di- and tripeptides it is shown t ... | 1980 | 6789520 |
| structure of the complex formed between the bacterial-produced inhibitor chymostatin and the serine enzyme streptomyces griseus protease a. | | 1980 | 6790710 |
| directed biosynthesis of new indolmycins. | tryptophan in a concentration of 0.4 microgram/ml increased the production of indolmycin by 37%. the lipophilic character of indolmycin was reduced via directed biosynthesis by substituting the aromatic ring system with a methoxy or hydroxy group in the 5-position of the antibiotic. this substitution was achieved by the addition of the corresponding tryptophan and indole precursors to a growing culture of streptomyces griseus atcc 12648. the more hydrophilic indolmycin derivatives displayed a mo ... | 1981 | 6792175 |
| [new type of sporulation regulation and streptomycin biosynthesis in secondary streptomyces griseus mutants]. | | 1981 | 6792249 |
| a sepharose derivative coupled with a leupeptin-like peptide aldehyde, glycylglycyl-l-argininal, and its use as an affinity adsorbent for trypsin. | a sepharose derivative containing a peptide aldehyde, glycylglycyl-l-argininal, the structure of which resembles that of leupeptin was prepared. it was a strong affinity adsorbent for trypsin (ec 3.4.21.4). bovine trypsin showed higher affinity for this adsorbent at the optimum ph of catalysis (8.2) than at lower ph (5.0). this observation was in good agreement with the ph dependence of the interaction of leupeptin and trypsin (kuramochi, h., nakata, h. and ishii, s. (1979) j. biochem. 86, 1403- ... | 1981 | 6793074 |
| evidence against a general role of nadp-glycohydrolase in differentiation of streptomyces griseus. | | 1981 | 6796557 |
| fredericamycin a, a new antitumor antibiotic. i. production, isolation and physicochemical properties. | a new antitumor antibiotic, fredericamycin a (fcrc-a48, nsc-305263), has been isolated from a strain of streptomyces griseus (fcrc-48). based on its unique ultraviolet-visible spectrum, infrared spectrum, proton and carbon-13 nuclear magnetic resonance spectra and mass spectra, it is judged to be a novel acid-base indicator type of compound. its production, isolation and physicochemical properties are discussed. the isolation, ultraviolet-visible spectrum and some biological properties of two mi ... | 1981 | 6798004 |
| studies on trypsin inhibitors of streptomyces griseus cal. | | 1981 | 6800933 |
| [leukaemomycin-blocked mutants of streptomyces griseus and their pigments]. | in a continued search for leukaemomycin-blocked mutants of three leukaemomycin-producing strains imet ja 3933, imet ja 5142 and imet ja 5570 of streptomyces griseus, 32 mutants producing aerial mycelium and spores were detected. furthermore, in all mutants cosynthetic capability has been observed. this report describes characterization of leukaemomycin-blocked mutants obtained by mutagenic treatment experiments using ntg and combined uv-/x-rays. according to the biosynthetic capability for anthr ... | 1981 | 6801872 |
| biochemical characteristics of non-streptomycin-producing mutants of streptomyces griseus. i. role of nad (p)-glycohydrolase in cell differentiation. | five non-streptomycin-producing mutants of an industrial strain of streptomyces griseus lacking aerial mycelium formation were compared with their genetic ancestor and another producing mutant with regard to the nad(p)-glycohydrolase activity during cultivation on different media. by contrast to producing strains, all the str- amy- mutants displayed much lower mycelial and extracellular levels of enzyme, thus confirming earlier contentions concerning its involvement in control by phospho-adenosi ... | 1981 | 6801874 |
| [interspecies recombination in streptomyces griseus, streptomyces olivaceus and streptomyces coelicolor]. | | 1982 | 6803104 |
| [circular permutation of dna molecules of streptomyces griseus kr actinophage pg2]. | dna molecules of phage pg2 have terminal repetitions and are partially circularly permuted. this was established by denaturation self-reannealing experiments and analysis of the distribution of denaturation loops in partially denatured pg2 dna molecules. the genome size is 40.8 kb, terminal repetitions are of about 2.2%, the degree of permutation is 35%. on the basis of the data on dna melting temperature the content of gc pairs was found equal to 65%. | 1982 | 6803124 |
| mutants blocked in streptomycin production in streptomyces griseus - the role of a-factor. | ninety-five streptomycin-nonproducing mutants derived from streptomyces griseus ft-1 by uv-irradiation could be classified into major two classes by cosynthesis tests. class i mutants (42 strains) were mutants blocked in the pathway of streptomycin biosynthesis while class ii mutants (49 strains) required a factor for streptomycin biosynthesis which was excreted by the parental or class i mutant strains. the factor could be replaced by synthetic a-factor (2s-isocapryloyl-3-s-hydroxymethyl-gamma- ... | 1982 | 6804428 |
| acyl chains of phospholipids and their variation with age in streptomyces griseus. | | 1981 | 6805427 |
| a screening method for autoregulators of anthracycline-producing streptomycetes. | a novel method was proposed for screening of autoregulators by anthracycline-producing strains of streptomyces griseus. by means of a modified cosynthesis procedure, an indicator strain was detected capable of producing both leukaemomycin and aerial mycelium only in the presence of specific inducing factors (autoregulators) which were produced not only by anthracycline-yielding organisms and their mutants, but also by a streptomycin-producing strain of streptomyces griseus as well as by its non- ... | 1982 | 6806999 |
| biochemical characteristics of non-streptomycin-producing mutants of streptomyces griseus. ii. lipids and fatty acid composition of vegetative mycelia. | five non-streptomycin-producing non-aerial-mycelium-forming mutants (str-amy-) of streptomyces griseus obtained either by spontaneous degeneration or during continuous cultivation of the high-producing aerial-mycelium-forming parent strain hp (str+amy+) were checked with regard to the composition of mycelial lipid material. all the str-amy- derivatives differed from their ancestor strain hp by an increased ration of 12-methyltetradecanoic acid (ac15:0) to isopalmitic acid (ic16:0) during growth ... | 1982 | 6807000 |
| isolation and structure of novel autoregulators from streptomyces griseus. | | 1982 | 6809723 |
| the effect of bacterial peptidoglycans on the immune response of hamsters to influenza virus vaccines. | the immune response of hamsters to influenza virus vaccine incorporating peptidoglycans derived from streptomyces griseus was investigated. the results showed that although inoculation of a subunit influenza virus vaccine together with a peptidoglycan elicited a markedly increased serum hi antibody response compared to that induced by the subunit vaccine alone, pre-treatment of animals with peptidoglycans resulted in a suppression of the antibody response to the vaccine haemagglutinin antigen. t ... | 1982 | 6811173 |
| comparative studies on the structure of active sites. behavior of "inverse substrates" toward trypsin and related enzymes. | the kinetics of hydrolysis of "inverse substrates," p-amidinophenyl alkanoates, catalyzed by urokinase, plasmin, kallikrein, and trypsins from various sources were studied. dissociation constants of acyl enzyme-ligand complexes, which are a characteristic parameter of the reaction with "inverse substrates," were analyzed with a view to comparing the spatial requirements of active sites. it was concluded that the spatial restraint of the active site as regards coexistence of the acyl residue and ... | 1982 | 6811567 |
| [detection of plasmid dna in different strains of streptomyces griseus]. | | 1982 | 6813654 |
| c-19393 e5, a new carbapenem antibiotic. fermentation, isolation and structure. | a new carbapenem antibiotic, c-19393 e5, was isolated from the culture filtrate of streptomyces griseus subsp. cryophilus c-19393 as a minor component. the chemical structure of the antibiotic was determined by comparing its spectral data with those of the known 5,6-cis carbapenem antibiotics and confirmed by partial synthesis from epithienamycin b as shown in fig. 1. the antibiotic has a broad antimicrobial spectrum and shows strong inhibitory activity against beta-lactamases. | 1982 | 6815148 |
| [chromosome mapping of streptomyces griseus 773 using interspecies recombination]. | | 1982 | 6815431 |
| pathways of phosphatidylethanolamine catabolism in streptomyces griseus. | | 1982 | 6816719 |
| nucleotide sequence of streptomyces griseus initiator trna. | the primary structure of initiator trna from streptomyces griseus was determined by post-labeling procedures. the nucleotide sequence is pc-g-c-g-g-g-g-u-g-g-a-g-c-a-g-c-u-c-g-g-d-a-g-c-u-c-g-c-u-g-g-g-c-u-c-a-u-a-a-c-c- c-a-g-a-g-g-u-c-g-c-a-g-g-u-psi-c-a-m1a-a-u-c-c-u-g-u-c-c-c-c-g-c-u-a-c-c-a0h. the unique feature of the sequence of this trna is that residue 54 is occupied by unmodified u, while ribothymidine is located in that position in most initiator trnas from eubacteria. | 1982 | 6817303 |
| [effect of nonmutagenic doses of 1,4-bis-diazoacetylbutane on streptomyces griseus]. | streptomyces griseus 15 was subjected to the action of 1,4-bis-diazoacetyl butane (dab) taken at concentrations of 10 to 50,000 micrograms/ml. small doses (10-100 micrograms/ml) of dab had no mutagenic action and activated the cultural growth (the viability and the survival rate of spores increased on solid media, while the biomass yield rised in liquid media). experiments were conducted using the method of orthogonal planning of a bifactorial experiment, and the role of the exposure time rised ... | 1982 | 6818438 |
| udp-n-methyl-d-glucosamine-phosphate--a possible intermediate of n-methyl-l-glucosamine moiety of streptomycin. | the formation of n-methyl-l-glucosamine moiety of streptomycin from d-glucosamine by streptomyces griseus was studied. the addition of thymine to the culture medium stimulated the formation of streptomycin and the incorporation of d-glucosamine into n-methyl-l-glucosamine moiety. during a study of sugar nucleotides in the mycelia, a novel udp-amino sugar was isolated. the compound was formed before the maximum production of streptomycin. it was udp-n-methyl-d-glucosamine-phosphate. | 1982 | 6819281 |
| genetic segregation in a high-yielding streptomycin-producing strain of streptomyces griseus. | the streptomycin-producing streptomyces griseus hp spontaneously segregated non-reverting derivatives with altered phenotypes. clones characterized by increased spore formation and decreased streptomycin production were found. two other types of derivatives were defective in aerial mycelium and streptomycin formation as well, but differed in the capacity to synthesize a yellow pigment. these derivatives were examined with respect to further properties. the stability of s. griseus hp was investig ... | 1982 | 6819723 |