| experimental intra-abdominal abscesses in rats: development of an experimental model. | an animal model has been developed to study the evolution of intra-abdominal abscesses. gelatin capsules containing pooled colonic contents and barium sulfate were prepared in an anaerobic chamber and implanted into the pelvic region of wistar rats. the natural course of the ensuing disease was studied in various groups according to the source of the inoculum and sex of the recipient. colonic contents derived from rats fed a grain diet produced a highly lethal disease with an 80% mortality rate ... | 1974 | 4611922 |
| d-ribulose-1, 5-diphosphate carboxylase and the evolution of autotrophy. | | 1974 | 4613398 |
| biohydrogenation of sterols and fatty acids by the intestinal microflora. | | 1974 | 4614666 |
| bacteriology of the gut and its clinical implications. | the bacteriology of the gastrointestinal tract is rapidly changing in laboratory techniques and clinical correlations. the flora is found to be very complex, predominantly anaerobic, and importantly dependent on diet. an etiologic role for colon bacteria in colon cancer is suggested by correlations between epidemiologic data and prevalent dietary patterns and stool culture findings. cultures from aspiration pneumonia, subphrenic abscess, and other intra-abdominal sepsis all yield anaerobes, and ... | 1974 | 4617971 |
| simplified gas chromatographic procedure for identification of bacterial metabolic products. | a rapid and simple procedure is described for analysis of fermentation products from anaerobic bacteria grown in glucose broth. a 1-ml sample of the culture is drained through cation-exchange resin in a pasteur pipette. the effluent fluid is directly analyzed isothermally in a gas chromatograph for volatile fatty acids (c(2) to c(6)) as well as for lactic, pyruvic, and succinic acids. this procedure is considered to be suitable for routine use in clinical bacteriology. | 1973 | 4632854 |
| a prospective study of infections in penetrating abdominal trauma. | | 1972 | 4639751 |
| eubacterium fissicatena sp.nov. isolated from the alimentary tract of the goat. | | 1972 | 4647469 |
| diet and the equilibrium between bacteria and yeast implanted in gnotobiotic rats. | | 1972 | 4674277 |
| regulation of aspartokinase activity in the genus beneckea and marine, luminous bacteria. | | 1973 | 4709503 |
| geographical differences in the bacterial flora of the gut. | | 1973 | 4718563 |
| biohydrogenation of sterols by eubacterium atcc 21,408--nova species. | | 1973 | 4730962 |
| nature of fecal sterols and intestinal bacterial flora. | | 1973 | 4735170 |
| utilization of the intermediate products of the decomposition of pectin and of galacturonic acid by pure strains of rumen bacteria. | | 1973 | 4735641 |
| structure of the polysaccharide antigen of eubacterium saburreum, strain l44. | | 1974 | 4854438 |
| tryptophan biosynthesis from indole-3-acetic acid by anaerobic bacteria from the rumen. | microbes in ruminal contents incorporated (14)c into cells when they were incubated in vitro in the presence of [(14)c]carboxyl-labeled indole-3-acetic acid (iaa). most of the cellular (14)c was found to be in tryptophan from the protein fractions of the cells. pure cultures of several important ruminal species did not incorporate labeled iaa, but all four strains of ruminococcus albus tested utilized iaa for tryptophan synthesis. r. albus did not incorporate (14)c into tryptophan during growth ... | 1974 | 4855566 |
| [studies on a bacterial hemophilus-x-factor. ii. distribution of the growth factor in heterotrophic bacteria]. | | 1966 | 4870052 |
| normal flora--rumen bacteria. | | 1970 | 4920104 |
| phenylethyl alcohol agar medium for isolation of anaerobic bacteria. | | 1970 | 4927090 |
| the anaerobic bacterial flora of the mouse cecum. | refinements of the anaerobic technique such as the utilization of prereduced media and the removal of traces of oxygen from the gases used by passage through a hot copper oven resulted in quantitative and qualitative improvements in the recovery of anaerobic bacteria from the cecum of spf mice. the commonest morphologic types of bacteria in the mouse cecum were tapered rods. these characteristically gave "speckled" colonies on agar plates. those identified were species of the genera fusobacteriu ... | 1970 | 4927597 |
| determination of bile acid conversion potencies of intestinal bacteria by screening in vitro and subsequent establishment in germfree rats. | | 1971 | 4938676 |
| the microbial flora of the rumen of cows fed hay and high cereal rations and its relationship to the rumen fermentation. | | 1971 | 4939274 |
| rifampin-blood-agar as a selective medium for the isolation of certain anaerobic bacteria. | a selective medium which allows detection of relatively small numbers of fusobacterium varium in fecal specimens is described. blood-agar containing 50 mug of rifampin per ml inhibits the growth of many species of bacteriodes and of f. fusi-forme/nucleatum but allows good growth of f. varium and most strains of f. mortiferum. quantitative cultures of 11 fecal specimens were done on rifampin and other selective and nonselective media. f. varium was recovered in counts of 10(6) and 10(7) per gram ... | 1971 | 4943582 |
| anaerobic bacteria on the mucosal epithelium of the murine large bowel. | anaerobic bacteria can be detected at population levels of 10(11) organisms per g of cecum or colon in adult mice from four different colonies widely spaced in the united states. most of these microorganisms are oxygen-intolerant fusiform-shaped bacteria. at least one type of these tapered, rod-shaped bacteria can be seen in layers in the epithelial mucin in frozen-section histological preparations of the large bowels of mice. in addition, such microorganisms can be seen within 0.5 mum of the ep ... | 1971 | 4949504 |
| an electron microscope study of thin sections of haemophilus vaginalis (gardner and dukes) and some possibly related species. | | 1966 | 4960378 |
| [association of a eubacterium in the life cycle of a myxobacterium: chondromyces apiculatus thaxter]. | | 1967 | 4963605 |
| [case of subacute bacterial endocarditis due to eubacterium]. | | 1968 | 4973741 |
| viable bacteria inside the rumen ciliate entodinium caudatum. | | 1969 | 4978363 |
| an enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase. | the distribution of catalase and superoxide dismutase has been examined in various micro-organisms. strict anaerobes exhibited no superoxide dismutase and, generally, no catalase activity. all aerobic organisms containing cytochrome systems were found to contain both superoxide dismutase and catalase. aerotolerant anaerobes, which survive exposure to air and metabolize oxygen to a limited extent but do not contain cytochrome systems, were found to be devoid of catalase activity but did exhibit s ... | 1971 | 4995818 |
| a polysaccharide antigen of an anaerobic oral filamentous microorganism (eubacterium saburreum) containing heptose and 0-acetyl as main constituents. 1. isolation and chemical characterization of the antigen. | | 1971 | 5003329 |
| total synthesis of acetate from co 2 . v. determination by mass analysis of the different types of acetate formed from 13 co 2 by heterotrophic bacteria. | mass analysis was used to determine the amount of acetate which is totally synthesized from (13)co(2) during fermentations by clostridium formicoaceticum, c. acidiurici, c. cylindrosporum, butyribacterium rettgeri, and diplococcus glycinophilus. in the fermentation of fructose by c. formicoaceticum, 27% of the acetate was found to be totally synthesized from co(2), and the remaining acetate was unlabeled, having been formed from fructose. evidence is presented that the purine-fermenting organism ... | 1972 | 5058447 |
| anaerobic bacteremia. | | 1972 | 5073944 |
| a cytochemical study of acid phosphatases and esterases during the induction of crown gall in the tomato. lycopersicon esculentum. | | 1971 | 5099263 |
| [significance of anaerobic bacteria isolated from the urinary tract]. | | 1971 | 5167893 |
| a comparison of the lytic action of cytophaga johnsonii on a eubacterium and a yeast. | | 1967 | 5298433 |
| 2-aminotyrosine, an analog of tyrosine. | | 1966 | 5339568 |
| [trichomonas vaginitis and accompanying microflora]. | | 1969 | 5349952 |
| isolation and identification of rumen bacteria capable of anaerobic rutin degradation. | | 1969 | 5392469 |
| growth of the riboflavin-splitting rumen organism eubacterium fissicatena in the presence of tunstate and molybdate. | | 1970 | 5500290 |
| carbon dioxide requirement of various species of rumen bacteria. | the carbon dioxide requirement of 32 strains of rumen bacteria, representing 11 different species, was studied in detail. increasing concentrations of co(2) were added as nahco(3) to a specially prepared co(2)-free medium which was tubed and inoculated under nitrogen. prior depletion of co(2) in the inoculum was found to affect the level of requirement; however, the complexity and buffering capacity of the medium did not appear to be involved. an absolute requirement for co(2) was observed for e ... | 1971 | 5541030 |
| [colonies of bacteria in the conjunctival sac of healthy humans, with special reference to anaerobic bacteria]. | | 1967 | 5625243 |
| the effect of coliform bacilli upon ureteral peristalsis. | | 1968 | 5644438 |
| the effect of coliform bacilli upon ureteral peristalsis. | | 1968 | 5651063 |
| [the use of products of microorganism vital activity in obtaining an anti-tumor vaccine]. | | 1968 | 5665403 |
| the 6-o-methylglucose-containig lipopolysaccharide of mycobacterium phlei. complet structure of the polysaccharide. | | 1968 | 5679967 |
| the peptidoglycan in walls of butyribacterium rettgeri. | | 1969 | 5777324 |
| cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay. | | 1969 | 5783892 |
| extent of solubilization of alpha-cellulose and hemicellulose of low-protein teff hay by pure cultures of cellulolytic rumen bacteria. | | 1969 | 5783893 |
| numerical taxonomy of some named coryneform bacteria. | | 1969 | 5792675 |
| characterization of several bovine rumen bacteria isolated with a xylan medium. | dehority, b. a. (ohio agricultural research and development center, wooster). characterization of several bovine rumen bacteria isolated with a xylan medium. j. bacteriol. 91:1724-1729. 1966.-studies were conducted to characterize eight strains of bacteria isolated from bovine rumen contents, by use of a medium containing xylan as the only added carbohydrate source. based on morphology, biochemical reactions, nutritional requirements, and fermentation products, five of the eight strains were ide ... | 1966 | 5937235 |
| the amino acid sequence of the serine containing murein of butybacterium rettgeri. | | 1966 | 5965240 |
| the synthesis of amino acids by methanobacterium omelianskii. | 1. methanobacterium omelianskii was grown on (14)co(2) and unlabelled ethanol, or on [1-(14)c]- or [2-(14)c]-ethanol and unlabelled carbon dioxide. the cell protein was hydrolysed and certain of the amino acids were isolated and degraded. 2. carbon from both carbon dioxide and ethanol is used for biosynthesis of amino acids, and in most cases ethanol is incorporated as a c(2) unit. ethanol carbon atoms and carbon dioxide carbon atoms apparently enter the same range of compounds. ethanol and carb ... | 1966 | 5965349 |
| [effect of ph medium on the vital activity of butylic bacteria]. | | 1966 | 5993614 |
| [demonstration of inhibitory substances elaborated by anaerobic non-sporulating bacteria]. | | 1966 | 5995564 |
| numerical taxonomy of gram-positive and catalase-positive rods isolated from frozen vegetables. | one hundred isolates from peas, beans, and corn were compared with cultures of corynebacterium, microbacterium, and arthrobacter by use of numerical taxonomic procedures. six groups (clusters), representing 75% of the isolates, resembled corynebacteriaceae. there was some doubt regarding the genera represented because the groups were not closely related to many of the known strains. the relationship of the different groups to each other, as well as a number of their properties, is presented. | 1967 | 6031430 |
| [antibacterial activity of mt-141 against anaerobic bacteria]. | in vitro and in vivo antibacterial activities of mt-141, a new cephamycin, against anaerobic bacteria were compared with those of cefmetazole (cmz), cefoxitin, cefotaxime, ceftizoxime, latamoxef and cefazolin to obtain the following results. mt-141 showed high activity against a wide variety of anaerobic bacteria including b. fragilis and c. difficile except for eubacterium lentum, e. aerofaciens and b. furcosus. antibacterial activity of mt-141 against anaerobic bacteria was almost independent ... | 1984 | 6092738 |
| do anaerobes cause urinary tract infection? | | 1980 | 6101823 |
| neutrophil chemotaxis induced by anaerobic bacteria isolated from necrotic dental pulps. | anaerobic bacteria belonging to the species bacteroides melaninogenicus, bacteroides asaccharolyticus, fusobacterium nucleatum, peptostreptococcus anaerobius and eubacterium alactolyticum, isolated from necrotic dental pulps, were tested for their capacity to induce neutrophil leukocyte chemotaxis. generation of chemotactic factors in serum by whole bacterial cells and chemotaxis induced by bacterial extracts were studied in vitro by the boyden technique. subcutaneously implanted wound chambers ... | 1980 | 6103573 |
| in vitro inhibition of neisseria gonorrhoeae growth by strict anaerobes. | thirty two anaerobic bacterial strains were screened for their in vitro inhibitory activity toward 21 neisseria gonorrhoeae strains, including two penicillin-resistant strains. from this screening, six anaerobic bacteria belonging to the genera bacteroides, peptostreptococcus, and eubacterium were selected for their large antigonococcal spectrum of activity. the inhibitory activity of these six strains appears to be specific to the gonococcus as a variety of anaerobes and aerobes were not genera ... | 1980 | 6105130 |
| nmr analyses on the molecular mechanism of the conformational rigidity of 2-thioribothymidine, a modified nucleoside in extreme thermophile trnas. | 1h-nmr analyses have been made on the conformations of 2-thioribothymidine (s2t), 2-thiodeoxyribothymidine (s2dt), as well as ribothymidine (t) and deoxyribothymidine (dt). s2t and s2dt exclusively take the anti form rather than the syn form. the c3'-endo-gg form of the sugar moiety is remarkably stabilized on modification of t to s2t, but not on modification of dt to s2dt. the steric effects of the 2-thiocarbonyl group and the 2'-hydroxyl group cause the rigidity of the c3'-endo-gg form of s2t. ... | 1983 | 6190675 |
| polyclonal b-cell activating capacities of gram-positive bacteria frequently isolated from periodontally diseased sites. | | 1982 | 6219204 |
| development of the normal gastrointestinal microflora of specific pathogen-free chickens. | the development of the normal intestinal microflora of the small intestine, caecum and large intestine of specific pathogen-free (spf) chickens, was studied in the period from hatching to 84 days of age. no bacteria were detected in any of the sites at hatchery (day 1), but by day 3 significant levels of faecal streptococci and coliforms were isolated from all sites. the flora of the small intestine was limited to faecal streptococci and coliforms for the first 40 days and then lactobacilli beca ... | 1984 | 6229572 |
| comparison of moxalactam (ly127935) and cefotaxime against anaerobic bacteria. | the in vitro activities of moxalactam (ly127935 [6059s]) and cefotaxime were compared with those of cefoxitin, cefamandole, cefuroxime, carbenicillin, and penicillin by agar dilution susceptibility testing of a variety of anaerobic bacteria. moxalactam proved to be the most active agent tested against bacteroides fragilis and other species of the b. fragilis group. moxalactam and cefotaxime showed activity similar to the other drugs against the remaining species of bacteroides, fusobacterium, ac ... | 1980 | 6249198 |
| [influence of cefotiam (sce-963, ctm) on bowel flora (author's transl)]. | the influence on bowel flora of ctm was studied in 5 children who were taking normal diet. 1) in the cases following no diarrhea, administration of ctm caused no significant changes in bowel flora. in the cases following diarrhea, administration of ctm caused a fall in coliform, bep group, lactobacillus and peptostreptococcus. however, after the administration was discontinued, the reduced bowel flora was returned to the normal range within a few days. 2) no overgrowth of bowel flora by pseudomo ... | 1981 | 6270414 |
| comparative evaluation of supplemented peptone broth with sodium polyanetholesulfonate and trypticase soy broth with sodium amylosulfate for detection of septicemia. | we compared the yield and speed of detection of clinically important microorganisms from 10,156 paired 5-ml samples of blood cultured in supplemented peptone broth (spb) with 0.03% sodium polyanetholesulfonate (sps) or trypticase soy broth (tsb) with 0.5% sodium amylosulfate (sas). the atmosphere of incubation (open venting units) and ratio of blood to broth (1:10) were the same for both samples. only cultures with adequate blood samples (greater than or equal to 80% of stated volume) were compa ... | 1982 | 6286716 |
| cecal microflora of turkeys fed low or high fiber diets: enumeration, identification, and determination of cellulolytic activity. | examination of cecal contents or bacterial cultures thereof from turkeys fed either a high fiber (hf) or low fiber (lf) ration indicated that direct microscopic counts of microbes were significantly higher in hf-fed than in lf-fed birds. there was no significant difference in mean colony counts between the two groups of turkeys. in both lf and hf-fed birds, 77% of the microbes were gram-positive rods, 14% gram-negative rods, and 9% gram-positive cocci. the predominant microorganism was eubacteri ... | 1983 | 6306633 |
| catabolism of diadenosine 5',5"'-p1,p4-tetraphosphate in procaryotes. purification and properties of diadenosine 5',5"'-p1,p4-tetraphosphate (symmetrical) pyrophosphohydrolase from escherichia coli k12. | enzymatic activity which hydrolyzes diadenosine 5',5"'-p1,p4-tetraphosphate (ap4a) yielding adp has been identified in extracts of eubacteria, escherichia coli and acidaminococcus fermentans, and of a highly thermophilic archaebacterium, pyrodictum occultum. specific ap4a (symmetric) pyrophosphohydrolase from escherichia coli k12 has been purified almost 400-fold. the preparation was free of phosphatase, atpase, phosphodiesterase, amp-nucleosidase, and adenylate kinase. the ap4a pyrophosphohydro ... | 1983 | 6317672 |
| the hydrogenation of gamma-linolenic acid by pure cultures of two rumen bacteria. | two species of rumen bacteria that have been previously shown to partially hydrogenate alpha-linolenic acid have been examined for their ability to hydrogenate gamma-linolenic acid. free gamma-linolenic acid is hydrogenated in vitro to stearic acid by a rumen fusocillus sp. (n.c.i.b. 11026), but only to cis,trans-octadec-6,11-enoic acid by a butyrivibrio sp. the sequential hydrogenations are preceded by a delta 12-cis-delta 11-trans isomerization identical with that observed in the hydrogenation ... | 1983 | 6318740 |
| susceptibility testing of anaerobic bacteria. | with the significant increase in resistance of anaerobic bacteria to antimicrobial agents in recent years, susceptibility testing of these organisms becomes very important. in addition to survey studies, hospitals should be determining their local patterns, and therapy of at least the more serious infections should be guided by susceptibility tests. of the various tests available, those most suitable for smaller hospitals for guidance of patient management are the broth disc elution procedure an ... | 1983 | 6323094 |
| continuous-flow cultures as in vitro models of the ecology of large intestinal flora. | an anaerobic continuous-flow (cf) culture method has been developed which reproduces a number of bacterial interactions that occur in the large intestine of mice. these were determined in the following ways. (i) bacterial counts in smears stained with 37 specific fluorescent antisera showed that the numeric balance between 37 strict anaerobes isolated from conventional mice was maintained in cf culture of conventional mouse flora in the same manner as in conventional mice. (ii) mixed populations ... | 1983 | 6339387 |
| mechanisms that control bacterial populations in continuous-flow culture models of mouse large intestinal flora. | a previous study had established that anaerobic continuous-flow (cf) cultures of conventional mouse cecal flora were able to maintain the in vivo ecological balance among the indigenous bacterial species tested. this paper describes experiments designed to determine the mechanisms which control the population sizes of these species in such cf cultures. one strain each of escherichia coli, fusobacterium sp., and eubacterium sp. were studied. growth of these strains in filtrates of cf cultures was ... | 1983 | 6339388 |
| the survival of anaerobic bacteria at 4 degrees c and 22 degrees c on swabs in three transport systems. statistical evaluation by application of a variance component model. | the survival of anaerobic bacteria on swabs in three transport systems at 4 degrees c and 22 degrees c was evaluated. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), a plain cotton swab in a dry aerobic tube (da) and a plain cotton swab in an aerobic agar tube (aa). the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum and clostridium ra ... | 1983 | 6346789 |
| the recovery of anaerobic bacteria from swabs in three transport systems. statistical evaluation by application of a variance component model. | the recovery of six anaerobic species from swabs in three transport systems was quantitatively evaluated. the test strains were bacteroides fragilis, b. melaninogenicus ss. intermedius, fusobacterium necrophorum, peptostreptococcus anaerobius, eubacterium lentum, and clostridium ramosum. the transport systems were a charcoal-impregnated cotton swab in modified stuart's transport medium (ms), or a plain cotton swab in either a dry aerobic tube (da) or an aerobic agar tube (aa). swabs were charged ... | 1983 | 6346790 |
| the frequency and some characteristics of anaerobic bacteria isolated from various forms of bovine mastitis. | the prevalence of strictly anaerobic bacteria in the secretions from untreated cases of mastitis in lactating dairy cows was investigated. the study involved 147 friesland cows in 12 highveld herds. all herds yielded cows with anaerobic udder infections. no anaerobic bacteria were recovered from cows with normal quarters or those with latent aerobic infections. only anaerobes were present in 10% of so-called 'aseptic' mastitis cases. a variety of anaerobic organisms was isolated concurrently wit ... | 1983 | 6355474 |
| metabolism of one-carbon compounds by chemotrophic anaerobes. | | 1983 | 6364727 |
| effect of bile acid oxazoline derivatives on microorganisms participating in 7 alpha-hydroxyl epimerization of primary bile acids. | we tested bile acid oxazoline derivatives of chenodeoxycholic (cdc-ox), 7-ketolithocholic (7-klc-ox), ursodeoxycholic (udc-ox), and deoxycholic (dc-ox) as inhibitors of the 7-epimerization of the primary bile acids cholic acid (ca) and cdc in cultures of four species of bacteria and the human fecal flora. the organisms tested elaborate a 7 alpha- and/or 7 beta-hydroxysteroid dehydrogenase (hsdh); they were escherichia coli (7 alpha-hsdh), bacteroides fragilis (7 alpha-hsdh), clostridium absonum ... | 1983 | 6366102 |
| anaerobic oral and dental infection. | anaerobes make up a significant part of the oral and dental indigenous and pathogenic flora. their role in periodontal disease, root canal infections, infections of the hard and soft oral tissue, as well as their importance as foci for disseminated infectious disease is well established. despite the ubiquitous involvement of bacteria, significant progress in our understanding of specific microbial etiologies has occurred only in the past decade. estimates of the number of species recovered from ... | 1984 | 6372018 |
| anaerobic infections in childhood. | anaerobic bacteria are part of the normal flora of mucous membranes and outnumber aerobic bacteria in the oral cavity and gastrointestinal tract. anaerobes can be isolated from pediatric patients with various infections when appropriate techniques for transportation and cultivation of samples are employed. frequently anaerobes are isolated in combination with other facultative or aerobic bacteria. the genera or groups of anaerobes most frequently isolated from pyogenic infections in children are ... | 1984 | 6372028 |
| recovery of anaerobic bacteria from vented blood-culture bottles. | from january 1979, to july 1981, blood from each patient suspected of having bacteremia was collected in two bottles of commercially prepared tryptic soy broth; one bottle was vented for 24 hr to the atmosphere. bottles were incubated at 37 degrees c for a maximum of seven days and were examined daily for signs of growth. blind subcultures onto chocolate-agar plates were performed 18-24 hr after collection and again at 48 hr. for 63,106 bottles inoculated, a total of 4,788 strains were isolated, ... | 1984 | 6372038 |
| anaerobes as normal oral flora. | the human mouth provides a suitable habitat for numerous bacterial genera. anaerobic genera or genera that include anaerobic members found in the oral cavity are actinomyces, arachnia, bacteroides, bifidobacterium, eubacterium, fusobacterium, lactobacillus, leptotrichia, peptococcus, peptostreptococcus, propionibacterium, selenomonas, treponema, and veillonella. the incidence of anaerobes varies with age of the individual and with specific sites sampled. in edentulous infants, the incidence of a ... | 1984 | 6372039 |
| [growth of anaerobes of the upper small intestine using the glovebox technic]. | with 80 patients a microecologic analysis of the duodenal flora has been performed. due to the optimal cultivation technique with the glove-box more frequently as supposed till now, anaerobes with partial high numbers could be detected, and classified to different types of dysbiosis. a striking role among the isolated and classified gram-positive roots play the genera eubacterium, propionibacterium, actinomyces, and bifidobacterium. peptococcus and peptostreptococcus are to be found frequently, ... | 1984 | 6387500 |
| [chemotherapeutic mixtures for the selective growth of anaerobes]. | in order to find a selective medium for the cultivation of gram-positive spore-less rods several chemotherapeutics have been tested. by adding of 5 micrograms metronidazol/ml to brain-heart-infusion-supplemented agar as well as by putting metronidazol discs on the agar it became possible to cultivate selective test tribes. lincomycin (5 micrograms/ml), clindamycin (5 micrograms/ml) and novobiocin (5 micrograms/ml) inhibited almost all the test tribes. spectinomycin (5 micrograms/ml) and neomycin ... | 1984 | 6387502 |
| [methods of determining the sensitivity of anaerobic bacteria to antibacterial drugs]. | | 1984 | 6395798 |
| chemolithoautotrophic metabolism of anaerobic extremely thermophilic archaebacteria. | several types of extremely thermophilic archaebacteria have recently been isolated from solfataric water holes, hot springs and hot sea floors. it has been shown that some of them can live using sulphur respiration of reduced carbon substrates as a source of energy, a type of metabolism previously described for the eubacterium desulfuromonas. we report here that several extremely thermophilic archaebacteria can live with carbon dioxide as their sole carbon source, obtaining energy from the oxida ... | 1983 | 6401847 |
| occurrence of diphthamide in archaebacteria. | we examined the nature of the diphtheria toxin fragment a recognition site in the protein synthesis translocating factor present in cell-free preparations from the archaebacteria thermoplasma acidophilum and halobacterium halobium. in agreement with earlier work (m. kessel and f. klink, nature (london) 287:250-251, 1980), we found that extracts from these organisms contain a protein factor which is a substrate for the adp-ribosylation reaction catalyzed by diphtheria toxin fragment a. however, t ... | 1983 | 6402493 |
| metabolism of h2-co2, methanol, and glucose by butyribacterium methylotrophicum. | the fermentative metabolism of butyribacterium methylotrophicum grown on either h2-co2, methanol, glucose, or co is described. the following reaction stoichiometries were obtained: 1.00 h2 + 0.52 co2 leads to 0.22 acetate + 0.06 cell c; 1 methanol + 0.18 co2 + 0.01 acetate leads to 0.24 butyrate + 0.29 cell c; and 1.00 glucose leads to 0.31 co2 + 1.59 acetate + 0.21 butyrate + 0.13 h2 + 1.58 cell c. cell yields of 1.7 g (dry weight) per mol of h2, 8.2 g (dry weight) per mol of methanol, 42.7 g ( ... | 1983 | 6402496 |
| evolving ribosome structure: domains in archaebacteria, eubacteria, and eucaryotes. | | 1983 | 6407755 |
| single-carbon catabolism in acetogens: analysis of carbon flow in acetobacterium woodii and butyribacterium methylotrophicum by fermentation and 13c nuclear magnetic resonance measurement. | the catabolism of methanol, formate, or carbon monoxide to acetate or butyrate or both was examined in two acetogenic bacteria. butyribacterium methylotrophicum simultaneously transformed methanol and formate mainly to butyrate with concomitant h2 and co2 production and consumption. in contrast, methanol plus co was primarily converted to acetate, and only slight amounts of co2 were produced. in vivo 13c nuclear magnetic resonance analysis of [13c]methanol transformation by b. methylotrophicum i ... | 1983 | 6411684 |
| eubacterium brachy. reactivity in in vitro bone resorptive bioassay. | recent studies have demonstrated an association of eubacterium sp. with the subgingival microflora of patients with chronic periodontitis. one species, eubacterium brachy, was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. an extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. this antigen was isolated by methanol precipitation and purified by gel filtration. the purified extracel ... | 1984 | 6423804 |
| preliminary characterization of inhibitors of neisseria gonorrhoeae produced in vitro by eubacterium limosum. | among anaerobic bacteria normally found in the urogenital flora, eubacterium limosum was found to inhibit the in vitro growth of neisseria gonorrhoeae. the antigonococcal activity produced by e. limosum was soluble in methanol and in a chloroform--methanol mixture (30:70). the fraction soluble in chloroform--methanol (30:70) yielded eight absorbance peaks when chromatographed on bio-gel p-2 and the inhibitory activity was found in the first two peaks. this activity was not absorbed on deae sepha ... | 1984 | 6424921 |
| methylation of ribosomal proteins in bacteria: evidence of conserved modification of the eubacterial 50s subunit. | methylation of the 50s ribosomal proteins from bacillus stearothermophilus, bacillus subtilis, alteromonas espejiana, and halobacterium cutirubrum was measured after the cells were grown in the presence of [1-14c]methionine or [methyl-3h]methionine or both. two-dimensional polyacrylamide gel electrophoretic analysis revealed, in general, similar relative electrophoretic mobilities of the methylated proteins from each eubacterium studied. proteins known to be structurally and functionally homolog ... | 1984 | 6425271 |
| equilibria in 5-s ribosomal rna secondary structure. bulges and interior loops in 5-s rna secondary structure may serve as articulations for a flexible molecule. | the basic assumption in this paper is that the secondary structure of a 5-s ribosomal rna cannot be represented by a single model. we propose that the molecule can adopt, at least within the ribosome, a series of slightly different structures of nearly equal stability. the different structures arise from the existence of ambiguous base-pairing opportunities in bulged helices and the adjacent interior loops. in eubacterial 5-s rnas there is one such an area, in eukaryotic 5-s rnas two such areas ... | 1984 | 6432535 |
| hplc purification and preparation of antibodies to cholic acid-inducible polypeptides from eubacterium sp. v.p.i. 12708. | the role of bile acid-inducible polypeptides in 7-dehydroxylation was investigated in eubacterium sp. v.p.i. 12708. cholic acid-inducible bile acid 7 alpha-, 7 beta-dehydroxylase, and delta 6 reductase activities co-eluted from a gel filtration high performance liquid chromatography (hplc) column. antibody (ab) was prepared to these enzymatically active fractions, immunoadsorbed with uninduced cell extract coupled to sepharose 4b, and used for immunoprecipitation of [35s]-methionine-labeled poly ... | 1984 | 6442337 |
| purification, properties, and n-terminal amino acid sequence of certain 50s ribosomal subunit proteins from the archaebacterium halobacterium cutirubrum. | sixteen ribosomal proteins (r-proteins) from the 50s ribosomal subunit of the archaebacterium halobacterium cutirubrum have been purified and their amino acid composition and partial n-terminal amino acid sequence have been determined. these proteins as a group are much more acidic than the large subunit r-proteins from eubacteria or eukaryotes. little sequence homology is evident between the 50s subunit archaebacterial r-proteins and the equivalent proteins from the eubacterium escherichia coli ... | 1984 | 6467081 |
| production of menaquinones by intestinal anaerobes. | ninety intestinal organisms (71 isolates from fecal samples of neutropenic patients with cancer or from various sites in patients with intraabdominal infections and 19 control strains) were examined by reverse-phase thin-layer chromatography for their ability to produce menaquinones in vitro. menaquinones were found in all of 24 organisms of the bacteroides fragilis group. two other bacteroides species, bacteroides disiens and bacteroides bivius, also produced menaquinones. a single isolate of b ... | 1984 | 6470528 |
| reduction of 17-keto steroids by anaerobic microorganisms isolated from human fecal flora. | the 17-keto function of phenolic steroids is reduced by the following intestinal bacteria: eubacterium lentum, clostridium paraputrificum, clostridium j-1 and clostridium innocuum. the 17-keto group of androstenedione is reduced solely by bacteroides fragilis. | 1984 | 6477942 |
| bacteriology of experimental gingivitis in children. | children are more resistant to gingivitis than are adults. to determine possible differences in their periodontal floras, an experimental gingivitis study, identical in design to one reported earlier with young adults, was conducted with four 4- to 6-year-old children. the incidence of sites that developed gingival index scores of 2 in children was less than one-third of the incidence observed in adults. the composition of the flora of each child was statistically significantly different from th ... | 1984 | 6480100 |
| [calculous cholecystitis complicated by non-clostridial anaerobic infection]. | | 1984 | 6482202 |
| comparative efficacy of four antibiotics in anaerobic pulmonary infection. an experimental model in rabbits. | the efficacy of cefoxitin, mezlocillin, latamoxef and metronidazole in anaerobic lung infection was studied using a rabbit model. a mixture of bacteroides fragilis, peptococcus morbillorum, eubacterium lentum and fusobacterium nucleatum was inoculated transtracheally to produce infection within the lung. mezlocillin was most effective, achieving bacteriologic cure in 5 out of 8 animals. with cefoxitin therapy, 4 out of 8 became bacteriologically sterile. severe diarrhea with elevated titers of c ... | 1984 | 6488936 |
| induction in rats of serum agglutinins to eubacterium, peptostreptococcus and coprococcus species by the faecal flora from patients with crohn's disease and healthy subjects. | sera from patients with crohn's disease more often than those from other subjects contain agglutinins against anaerobic coccoid rods, identified as peptostreptococcus productus, eubacterium contortum (two strains) and coprococcus comes. the presence of antigens of the four coccoid rods in faeces of patients with crohn's disease and healthy subjects was investigated by inoculation of rats with faecal suspensions. antigens of the coccoid rods were detected in faeces from both patients with crohn's ... | 1984 | 6501880 |
| characterization of predominant bacteria from the colons of normal and dysenteric pigs. | bacterial populations adherent to the mucosa of the proximal colons of weaned, healthy pigs were compared with populations from pigs with dysentery induced by inoculation with a culture of treponema hyodysenteriae. isolates (136) representative of the predominant flora adherent to colonic epithelia of normal pigs and isolates (162) from pigs with dysentery were cultured anaerobically on a rumen fluid-based medium and characterized. most (71%) of the isolates from colonic epithelia of normal pigs ... | 1984 | 6508310 |