| bifidobacterium bifidum olb6378 simultaneously enhances systemic and mucosal humoral immunity in low birth weight infants: a non-randomized study. | probiotic supplementation has been part of the discussion on methods to enhance humoral immunity. administration of bifidobacterium bifidum olb6378 (olb6378) reduced the incidence of late-onset sepsis in infants. in this non-randomized study, we aimed to determine the effect of administration of live olb6378 on infants' humoral immunity. secondly, we tried to elucidate whether similar effects would be observed with administration of non-live olb6378. low birth weight (lbw) infants weighing 1500- ... | 2017 | 28245626 |
| development of strain-specific primers for identification of bifidobacterium bifidum bgn4. | bifidobacterium bifidum bgn4 (bgn4) has many proven beneficial effects including antiallergy and anti-cancer properties. it has been commercialized and used in several probiotic products and thus, strain-specific identification of this strain is very valuable for further strain dependent physiological study. for this purpose, we developed novel multiplex polymerase chain reaction (pcr) primer sets for strain specific detection of bgn4 in the commercial product and fecal sample of animal model. t ... | 2017 | 28237995 |
| effects of probiotics on nonalcoholic fatty liver disease in obese children and adolescents. | this study aims to evaluate the effects of some probiotics on sonographic and biochemical nonalcoholic fatty liver disease (nafld). | 2017 | 28230607 |
| probiotics (lactobacillus gasseri ks-13, bifidobacterium bifidum g9-1, and bifidobacterium longum mm-2) improve rhinoconjunctivitis-specific quality of life in individuals with seasonal allergies: a double-blind, placebo-controlled, randomized trial. | background: rhinoconjunctivitis-specific quality of life is often reduced during seasonal allergies. the mini rhinoconjunctivitis quality of life questionnaire (mrqlq) is a validated tool used to measure quality of life in people experiencing allergies (0 = not troubled to 6 = extremely troubled). probiotics may improve quality of life during allergy season by increasing the percentage of regulatory t cells (tregs) and inducing tolerance.objective: the objective of this study was to determine wh ... | 2017 | 28228426 |
| immunomodulatory effects of bifidobacterium bifidum 231 on trinitrobenzenesulfonic acid-induced ulcerative colitis in rats. | ulcerative colitis and crohn's disease are two important chronic inflammatory bowel diseases (ibd) characterized by prominent intestinal inflammation. probiotics are the bacteria that promote the host health by its immunomodulatory activity. the present study investigated the correlation between in vitro adhesion and immunomodulatory properties, and to assess the therapeutic potential of bifidobacterium bifidum 231 (bif 231), a new strain of probiotic in ulcerative colitis in rats. | 2017 | 28159236 |
| studying vertical microbiome transmission from mothers to infants by strain-level metagenomic profiling. | the gut microbiome becomes shaped in the first days of life and continues to increase its diversity during the first months. links between the configuration of the infant gut microbiome and infant health are being shown, but a comprehensive strain-level assessment of microbes vertically transmitted from mother to infant is still missing. we collected fecal and breast milk samples from multiple mother-infant pairs during the first year of life and applied shotgun metagenomic sequencing followed b ... | 2017 | 28144631 |
| genome-wide immune modulation of tlr3-mediated inflammation in intestinal epithelial cells differs between single and multi-strain probiotic combination. | genome-wide transcriptional analysis in intestinal epithelial cells (iec) can aid in elucidating the impact of single versus multi-strain probiotic combinations on immunological and cellular mechanisms of action. in this study we used human expression microarray chips in an in vitro intestinal epithelial cell model to investigate the impact of three probiotic bacteria, lactobacillus helveticus r0052 (lh-r0052), bifidobacterium longum subsp. infantis r0033 (bl-r0033) and bifidobacterium bifidum r ... | 2017 | 28099447 |
| antifungal activity and aflatoxin degradation of bifidobacterium bifidum and lactobacillus fermentum against toxigenic aspergillus parasiticus. | food and feedstuff contamination with aflatoxins (afts) is a serious health problem for humans and animals, especially in developing countries. the present study evaluated antifungal activities of two lactic acid bacteria (lab) against growth and aflatoxin production of toxigenic aspergillus parasiticus. the mycelial growth inhibition rate of a. parasiticus ptcc 5286 was investigated in the presence of bifidobacterium bifidum ptcc 1644 and lactobacillus fermentum ptcc 1744 by the pour plate meth ... | 2016 | 28077976 |
| the changes of proteins fractions shares in milk and fermented milk drinks. | the aim of this research was to observe the changes which take place in the electrophoretic picture of milk proteins after pasteurisation and inoculation with different starter cultures (both traditional and probiotic). after incubation, the yoghurt, kefir, acidified milk, fermented bifidobacterium bifidum drink and lactobacillus acidophillus drink were chilled for 14 days to observe the changes which occurred. | 2017 | 28071015 |
| fermentation optimization of goat milk with lactobacillus acidophilus and bifidobacterium bifidum by box-behnken design. | goat milk is only limited to the processing of goat milk powder and liquid milk, the products are mainly about milk powder and a few of them are made as milk tablet. therefore, the study of probiotic goat milk will have great significance in the full use of goats and the development of the goat milk industry in china. | 2017 | 28071005 |
| a probiotic combination attenuates experimental colitis through inhibition of innate cytokine production. | inflammatory bowel disease (ibd) is a severe immune cell-mediated syndrome characterised by extensive inflammatory and effector mucosal responses leading to tissue destruction in the colon and small intestine. the leading hypothesis is that dysbiosis of the gut flora causes an excessive immune response and inflammation in the gastrointestinal track. lactic acid bacteria (lab) can correct dysbiosis of the normal microbiota. in the current study, the therapeutic potential of seven lab strains in c ... | 2016 | 28008786 |
| the additional effects of a probiotic mix on abdominal adiposity and antioxidant status: a double-blind, randomized trial. | to investigate whether a probiotic mix has additional effects when compared with an isolated dietary intervention on the body composition, lipid profile, endotoxemia, inflammation, and antioxidant profile. | 2017 | 28008750 |
| metformin is associated with higher relative abundance of mucin-degrading akkermansia muciniphila and several short-chain fatty acid-producing microbiota in the gut. | recent studies suggest the beneficial effects of metformin on glucose metabolism may be microbially mediated. we examined the association of type 2 diabetes, metformin, and gut microbiota in community-dwelling colombian adults. on the basis of previous research, we hypothesized that metformin is associated with higher levels of short-chain fatty acid (scfa)-producing and mucin-degrading microbiota. | 2017 | 27999002 |
| probiotic supplementation in diabetic hemodialysis patients has beneficial metabolic effects. | this study determined the effects of probiotic supplementation on glycemic control, lipid concentrations, biomarkers of inflammation and oxidative stress in 60 diabetic patients on hemodialysis in a parallel randomized double-blind placebo-controlled clinical trial. participants were initially matched based on sex, duration of dialysis and diabetes, body mass index and age. subsequently, they were randomly divided into two groups to take either a capsule containing the probiotics lactobacillus a ... | 2017 | 27927601 |
| evaluation of prebiotic effects of high-purity galactooligosaccharides in vitro and in vivo. | galactooligosaccharides (gos) are an important class of dietary prebiotics that exert beneficial effects on intestinal microbiota and gut barrier function. in this study, high-purity gos (hp-gos) were investigated in vitro and in vivo and confirmed as prebiotic ingredients in rat diet. hp-gos were successfully produced using a two-step process, enzymatic hydrolysis and fermentation by yeast. they were found to serve as a good substrate and carbon source for supporting the growth of probiotic bac ... | 2016 | 27904405 |
| effect of probiotic supplementation on cognitive function and metabolic status in alzheimer's disease: a randomized, double-blind and controlled trial. | alzheimer's disease (ad) is associated with severe cognitive impairments as well as some metabolic defects. scant studies in animal models indicate a link between probiotics and cognitive function. this randomized, double-blind, and controlled clinical trial was conducted among 60 ad patients to assess the effects of probiotic supplementation on cognitive function and metabolic status. the patients were randomly divided into two groups (n = 30 in each group) treating with either milk (control gr ... | 2016 | 27891089 |
| probiotic supplements beneficially affect tryptophan-kynurenine metabolism and reduce the incidence of upper respiratory tract infections in trained athletes: a randomized, double-blinded, placebo-controlled trial. | prolonged intense exercise has been associated with transient suppression of immune function and an increased risk of infections. in this context, the catabolism of amino acid tryptophan via kynurenine may play an important role. the present study examined the effect of a probiotic supplement on the incidence of upper respiratory tract infections (urti) and the metabolism of aromatic amino acids after exhaustive aerobic exercise in trained athletes during three months of winter training. | 2016 | 27886064 |
| time- and strain-specific downregulation of intestinal epas1 via mir-148a by bifidobacterium bifidum. | bifidobacteria play a role in intestinal homeostasis but molecular mechanisms remain underinvestigated. the aim of this study was to assess if probiotic bifidobacterium strains alter expression of intestinal microrna and downstream target gene response. | 2016 | 27883285 |
| application study of 1,2-α-l-fucosynthase: introduction of fucα1-2gal disaccharide structures on n-glycan, ganglioside, and xyloglucan oligosaccharide. | we have recently generated a highly efficient 1,2-α-l-fucosynthase (bbafca n423h mutant) by protein engineering of 1,2-α-l-fucosidase from bifidobacterium bifidum jcm 1254. this synthase could specifically introduce h-antigens (fucα1-2gal) into the non-reducing ends of oligosaccharides and in o-linked glycans in mucin glycoprotein. in the present study, we show an extended application of the engineered 1,2-α-l-fucosynthase by demonstrating its ability to insert fuc residues into n- and o-glycans ... | 2017 | 27832720 |
| [probiotic associations in the prevention of necrotising enterocolitis and the reduction of late-onset sepsis and neonatal mortality in preterm infants under 1,500g: a systematic review]. | necrotising enterocolitis (nec) is one of the most common and serious acquired bowel diseases a premature newborn can face. this meta-analysis was performed comparing different probiotic mixtures to ascertain their benefits as a routine tool for preventing necrotising enterocolitis and reducing late-onset sepsis and mortality in premature neonates of less than 1500g. | 2016 | 26611880 |
| anti-obese effects of two lactobacilli and two bifidobacteria on icr mice fed on a high fat diet. | previous researchers have documented that probiotic bacteria can have anti-obesity effects on mice fed a high fat diet (hfd) and improve metabolic syndrome. the beneficial effects of the probiotic bacteria are suggested to be strain dependent. in this study, two candidate lactobacteria strains, lactobacillus casei ibs041, lactobacillus acidophilus ad031 and two bifidobacteria strains, bifidobacterium bifidum bgn4 and bifidobacterium longum bori, were individually administered to hfd-fed mice for ... | 2016 | 27746173 |
| the effects of synbiotic supplementation on markers of insulin metabolism and lipid profiles in gestational diabetes: a randomised, double-blind, placebo-controlled trial. | to the best of our knowledge, data on the effects of synbiotic supplementation on markers of insulin metabolism and lipid concentrations in patients with gestational diabetes mellitus (gdm) are scarce. the aim of the current study was to determine the effects of synbiotic supplementation on markers of insulin metabolism and lipid profiles in gdm patients. in total, seventy patients with gdm aged 18-40 years were assigned to two groups - the synbiotic group (n 35) and the placebo group (n 35) - i ... | 2016 | 27681077 |
| crystal structure of β1→6-galactosidase from bifidobacterium bifidum s17: trimeric architecture, molecular determinants of the enzymatic activity and its inhibition by α-galactose. | in a search for better comprehension of β-galactosidase function and specificity, we solved the crystal structures of the gh42 β-galactosidase bbgii from bifidobacterium bifidum s17, a well-adapted probiotic microorganism from the human digestive tract, and its complex with d-α-galactose. bbgii is a three-domain molecule that forms barrel-shaped trimers in solution. bbgii interactions with d-α-galactose, a competitive inhibitor, showed a number of residues that are involved in the coordination o ... | 2016 | 27685756 |
| effects of probiotic supplementation on metabolic status in pregnant women: a randomized, double-blind, placebo-controlled trial. | limited data is available on the effects of multispecies probiotic supplementation on metabolic status in pregnant women in the first half of pregnancy. the current study was carried out to determine the effects of multispecies probiotic capsule supplementation on metabolic status among pregnant women in the first half of pregnancy. | 2016 | 27743432 |
| quantification of microbial uptake of quercetin and its derivatives using an uhplc-esi-qtof mass spectrometry assay. | a liquid chromatography-mass spectrometry-based method for the quantification of intracellular concentrations of dietary phenolics in bacteria was developed. using this assay, the time-dependent uptake of quercetin and two of its glycosides into gut microbiota model organisms (escherichia coli and bifidobacterium bifidum) under aerobic and anaerobic conditions was studied. for the first time, quantitative data on the uptake of dietary phenols were obtained indicating a time-dependent differentia ... | 2016 | 27711903 |
| in ovo validation model to assess the efficacy of commercial prebiotics on broiler performance and oxidative stability of meat. | the purpose of this study was to examine the effect of in ovo injection of 2 different prebiotics, dinovo (dn; laminaria spp., extract containing laminarin and fucoidan) and bi(2)tos (bi; non-digestive trans-galactooligosaccharides from milk lactose digested with bifidobacterium bifidum ncimb 41171), on growth, slaughter traits, intramuscular fat percentage (if) and muscle fiber diameter, and lipid oxidation of meat in chickens reared under commercial conditions, following an in ovo trial protoc ... | 2017 | 27664196 |
| review on bifidobacterium bifidum bgn4: functionality and nutraceutical applications as a probiotic microorganism. | bifidobacterium bifidum bgn4 is a probiotic strain that has been used as a major ingredient to produce nutraceutical products and as a dairy starter since 2000. the various bio-functional effects and potential for industrial application of b. bifidum bgn4 has been characterized and proven by in vitro (i.e., phytochemical bio-catalysis, cell adhesion and anti-carcinogenic effects on cell lines, and immunomodulatory effects on immune cells), in vivo (i.e., suppressed allergic responses in mouse mo ... | 2016 | 27649150 |
| use of a collagen membrane to enhance the survival of primary intestinal epithelial cells. | intestinal epithelial cell culture is important for biological, functional and immunological studies. since enterocytes have a short in vivo life span due to anoikis, we aimed to establish a novel and reproducible method to prolong the survival of mouse and human cells. cells were isolated following a standard procedure, and cultured on ordered-cow's collagen membranes. a prolonged cell life span was achieved; cells covered the complete surface of bio-membranes and showed a classical enterocyte ... | 2016 | 27626762 |
| administration of lactobacillus casei and bifidobacterium bifidum ameliorated hyperglycemia, dyslipidemia, and oxidative stress in diabetic rats. | the present work was planned to evaluate the antihyperglycemic, lipid-lowering, and antioxidant effect of lactobacillus casei and bifidobacterium bifidum in streptozotocin (stz)-induced diabetic rats. | 2016 | 27625767 |
| elucidating the gut microbiome of ulcerative colitis: bifidobacteria as novel microbial biomarkers. | ulcerative colitis (uc) is associated with a substantial alteration of specific gut commensals, some of which may be involved in microbiota-mediated protection. in this study, microbiota cataloging of uc patients by 16s rrna microbial profiling revealed a marked reduction of bifidobacteria, in particular the bifidobacterium bifidum species, thus suggesting that this taxon plays a biological role in the aetiology of uc. we investigated this further through an in vivo trial by testing the effects ... | 2016 | 27604252 |
| necrotising enterocolitis and mortality in preterm infants after introduction of probiotics: a quasi-experimental study. | evidence on the clinical effectiveness of probiotics in the prevention of necrotising enterocolitis (nec) in preterm infants is conflicting and cohort studies lacked adjustment for time trend and feeding type. this study investigated the association between the introduction of routine probiotics (lactobacillus acidophilus and bifidobacterium bifidum; infloran(®)) on the primary outcome 'nec or death'. preterm infants (gestational age <32 weeks or birth weight <1500 gram) admitted before (jan 200 ... | 2016 | 27545195 |
| evolutionary architecture of the infant-adapted group of bifidobacterium species associated with the probiotic function. | bifidobacteria, often associated with the gastrointestinal tract of animals, are well known for their roles as probiotics. among the dozens of bifidobacterium species, bifidobacterium bifidum, b. breve, and b. longum are the ones most frequently isolated from the feces of infants and known to help the digestion of human milk oligosaccharides. to investigate the correlation between the metabolic properties of bifidobacteria and their phylogeny, we performed a phylogenomic analysis based on 452 co ... | 2016 | 27524178 |
| [probiotics as stimulators of immune response against pathogens in the respiratory tract]. | this review analyzes whether.it is expedient to use oral probiotics for the stimulation of immune response against pathogens in the respiratory tract. it considers a relationship between.mucosal microbial colonization in different biotopes of the body and mucosal.immunity in the respiratory tract. the principal and terminological controversial issues of colonic dysbiosis and the possibilities of using the medicines and products containing live commensals/symbionts to correct microbiota disturban ... | 2016 | 27458629 |
| consumption of a bifidobacterium bifidum strain for 4 weeks modulates dominant intestinal bacterial taxa and fecal butyrate in healthy adults. | modulation of the intestinal microbial ecosystem (ime) is a useful target to establish probiotic efficacy in a healthy population. we conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of bifidobacterium bifidum strain bb on the ime of adult healthy volunteers of both sexes. high-throughput 16s rrna gene sequencing was used to characterize the fecal microbiota before and after 4 weeks of daily probiotic cell consumption. the intake ... | 2016 | 27451450 |
| bifidus milk powder: processing parameter standardization and shelf stability evaluation. | spray dried bifidus milk powder was prepared by supplementing cow milk with different level of additives to obtain slurry of desired concentration. the slurry was pasteurized, cooled and inoculated with bifidobacterium bifidum, incubated and dried to produce a bifidus milk powder. among the various bifidus milk powder prepared, the slurry of mention the level total soluble solids exhibited good organoleptic characteristics and it has been standardized for further analysis. moisture content, bulk ... | 2016 | 27413234 |
| study on the influence of tea extract on probiotics in skim milk: from probiotics propagation to metabolite. | in this study, the influence of tea extract (te) on the growth of probiotics in skim milk was examined. lactobacillus plantarum st-iii, bifidobacterium bifidum bb02, lactobacillus acidophilus ncfm, and lactobacillus rhamnosus gg were used in this study. the introduction of te in milk significantly stimulated the propagation and acidification of l. rhamnosus gg and l. acidophilus ncfm. the antioxidant capacities and the total free amino acid contents of all fermented milk products were enhanced b ... | 2016 | 27384493 |
| integration of genomic and proteomic data to identify candidate genes in ht-29 cells after incubation with bifidobacterium bifidum atcc 29521. | as the predominant group inhabiting the human gastrointestinal tract, bifidobacteria play a vital role in human nutrition, therapeutics, and health by shaping and maintaining the gut ecosystem, reducing blood cholesterol, and promoting the supply of nutrients. the interaction between bacterial cells and human intestinal epithelial cell lines has been studied for decades in an attempt to understand the mechanisms of action. these studies, however, have been limited by lack of genomic and proteomi ... | 2016 | 27372578 |
| [laboratory and experimental study of the complex probiotic preparation "bifilact-bils" in capsulated form]. | the composition and technology of complex probiotic in hard gelatin capsules was developed in perm branch "biomed" of "microgen" state company. the preparation contains three production strains: lactobacillus plantarum 8p-a3, l. acidophilus k3w24 and bifidobacterium bifidum 1. laboratory and experimental (preclinical) study of the probiotic included investigation of the antagonistic activity, "acute" and "chronic" toxicity, the effect of the preparation on histology and hematology of laboratory ... | 2016 | 27301138 |
| clinical trial/experimental study (consort compliant): optimal time period to achieve the effects on synbiotic-controlled wheezing and respiratory infections in young children. | urban life is often followed by immune dysfunction and loss of immune tolerance in the youngest children. | 2016 | 27276856 |
| biochemical engineering approaches for increasing viability and functionality of probiotic bacteria. | the literature presents a growing body of evidence demonstrating the positive effect of probiotics on health. probiotic consumption levels are rising quickly in the world despite the fluctuation of their viability and functionality. technological methods aiming at improving probiotic characteristics are thus highly wanted. however, microbial metabolic engineering toolbox is not available for this kind of application. on the other hand, basic microbiology teaches us that bacteria are able to exhi ... | 2016 | 27271598 |
| comparative evaluation of commercially available freeze dried powdered probiotics on mutans streptococci count: a randomized, double blind, clinical study. | probiotic approaches are being considered to eliminate pathogenic microorganisms and are an alternative and promising way to combat infections by using harmless bacteria to displace pathogenic microorganisms. the aim of this study was to evaluate the effectiveness of commercially available freeze dried powdered probiotics on mutans streptococci count among 12-15 year-old indian schoolchildren. | 2015 | 27252756 |
| a randomized controlled clinical trial investigating the effect of synbiotic administration on markers of insulin metabolism and lipid profiles in overweight type 2 diabetic patients with coronary heart disease. | objective: the current study was performed to evaluate the effects of synbiotic administration on metabolic profiles in overweight diabetic patients with coronary heart disease (chd). methods: this randomized, double-blind, placebo-controlled trial was done among 60 diabetic patients with chd. participants were randomly divided into 2 groups: group a (n=30) received synbiotic supplements containing 3 probiotic bacteria spices lactobacillus acidophilus 2×10(9), lactobacillus casei 2×10(9), bifido ... | 2017 | 27219886 |
| effects of probiotic supplementation on glycaemic control and lipid profiles in gestational diabetes: a randomized, double-blind, placebo-controlled trial. | to our knowledge, data on the effects of probiotic supplementation on glycaemic control and lipid concentrations in patients with gestational diabetes mellitus (gdm) are scarce. | 2016 | 27209439 |
| clinical and metabolic response to probiotic supplementation in patients with rheumatoid arthritis: a randomized, double-blind, placebo-controlled trial. | this study was performed to determine the effects of probiotic supplementation on clinical and metabolic status of patients with rheumatoid arthritis (ra). | 2016 | 27135916 |
| fermented acanthopanax koreanum root extract reduces uvb- and h2o2-induced senescence in human skin fibroblast cells. | the present study assessed the effects of an aqueous extract of acanthopanax koreanum root (ae) and of ae following fermentation by lactic acid bacteria (lactobacillus plantarum and bifidobacterium bifidum) (aef) on human skin fibroblast hs68 cells exposed to ultraviolet b (uvb) irradiation and oxidative stress. aef effectively antagonized the senescence-associated β-galactosidase staining and upregulation of p53 and p21(cip1/waf1) induced by uvb or h2o2 treatment in hs68 cells. it also exhibite ... | 2016 | 27090187 |
| crystal structure of a putative exo-β-1,3-galactanase from bifidobacterium bifidum s17. | given the current interest in second-generation biofuels, carbohydrate-active enzymes have become the most important tool to overcome the structural recalcitrance of the plant cell wall. while some glycoside hydrolase families have been exhaustively described, others remain poorly characterized, especially with regard to structural information. the family 43 glycoside hydrolases are a diverse group of inverting enzymes; the available structure information on these enzymes is mainly from xylosida ... | 2016 | 27050262 |
| th17 responses and natural igm antibodies are related to gut microbiota composition in systemic lupus erythematosus patients. | intestinal dysbiosis, characterized by a reduced firmicutes/bacteroidetes ratio, has been reported in systemic lupus erythematosus (sle) patients. in this study, in vitro cultures revealed that microbiota isolated from sle patient stool samples (sle-m) promoted lymphocyte activation and th17 differentiation from naïve cd4(+) lymphocytes to a greater extent than healthy control-microbiota. enrichment of sle-m with treg-inducing bacteria showed that a mixture of two clostridia strains significantl ... | 2016 | 27044888 |
| antimicrobial effect of probiotics on bacterial species from dental plaque. | the antimicrobial role of probiotic lactobacillus casei subspecies casei dg (l. casei dg) and of the mix culture of probiotic lactobacillus acidophilus la-5 and bifidobacterium bb-12 was tested on species of staphylococcus, streptococcus, pasteurella, and neisseria genera from supragingival sites from dogs with dental disease of different breed, age, sex, weight, and diet. the research was conducted on these four genera because of their importance in zoonotic infections after dog bites. | 2016 | 27031452 |
| lactobacillus rhamnosus gg increases toll-like receptor 3 gene expression in murine small intestine ex vivo and in vivo. | administration of lactobacillus rhamnosus gg (lgg) has been reported to be therapeutically effective against acute secretory diarrhoea resulting from the structural and functional intestinal mucosal lesions induced by rotavirus infection; however, the underlying mechanisms remain to be completely elucidated. because toll-like receptor 3 (tlr3) plays a key role in the innate immune responses following the recognition of rotavirus, the present study examined whether lgg influences tlr3 gene expres ... | 2016 | 27013459 |
| eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella. | listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ... | 2016 | 27002648 |
| living cells of probiotic bifidobacterium bifidum yit 10347 detected on gastric mucosa in humans. | the probiotic strain bifidobacterium bifidum yit 10347 has been demonstrated to inhibit helicobacter pylori activity, prevent injury to the gastric mucosa, and improve general gastric malaise symptoms in h. pylori positive patients. this study aimed to investigate the adhering activity and localisation of b. bifidum yit 10347 to gastric cells and tissue in vitro, and in human in vivo to clarify the mechanism of its beneficial effects on the stomach. the in vitro study found the adhesion rate of ... | 2016 | 26925600 |
| proteomic profiling of bifidobacterium bifidum s17 cultivated under in vitro conditions. | bifidobacteria are frequently used in probiotic food and dairy products. bifidobacterium bifidum s17 is a promising probiotic candidate strain that displays strong adhesion to intestinal epithelial cells and elicits potent anti-inflammatory capacity both in vitro and in murine models of colitis. the recently sequenced genome of b. bifidum s17 has a size of about 2.2 mb and encodes 1,782 predicted protein-coding genes. in the present study, a comprehensive proteomic profiling was carried out to i ... | 2016 | 26903976 |
| effect of salt stress on morphology and membrane composition of lactobacillus acidophilus, lactobacillus casei, and bifidobacterium bifidum, and their adhesion to human intestinal epithelial-like caco-2 cells. | the effects of nacl reduction (10.0, 7.5, 5.0, 2.5, and 0% nacl) and its substitution with kcl (50% substitution at each given concentration) on morphology of lactobacillus acidophilus, lactobacillus casei, and bifidobacterium longum was investigated using transmission electron microscopy. changes in membrane composition, including fatty acids and phospholipids, were investigated using gas chromatography and thin layer chromatography. adhesion ability of these bacteria to human intestinal epithe ... | 2016 | 26874411 |
| efficacy of oral bifidobacterium bifidum atcc 29521 on microflora and antioxidant in mice. | this study aimed to examine whether bifidobacterium bifidum atcc 29521, a species of colonic microflora in humans, is involved in the intestinal tract of mice. this study was also conducted to determine the antioxidant activity of this species by evaluating different microbial populations and reactive oxygen species isolated from feces and intestinal contents for 28 days of oral administration. microbial diversities were assessed through bacterial culture techniques, pcr-dgge, and real-time pcr. ... | 2016 | 26863255 |
| routine use of probiotics in preterm infants: longitudinal impact on the microbiome and metabolome. | probiotics are live microbial supplements that colonize the gut and potentially exert health benefit to the host. | 2016 | 26859305 |
| bifidobacterium bifidum r0071 decreases stress-associated diarrhoea-related symptoms and self-reported stress: a secondary analysis of a randomised trial. | psychological stress is associated with gastrointestinal (gi) distress. this secondary analysis from a randomised, double-blind, placebo-controlled study examined whether three different probiotics could normalise self-reported stress-associated gi discomfort and reduce overall self-reported stress. undergraduate students (n=581) received lactobacillus helveticus r0052, bifidobacterium longum ssp. infantis r0033, bifidobacterium bifidum r0071, or placebo. participants self-reported 2 outcomes fo ... | 2016 | 26839075 |
| probiotics (lactobacillus acidophilus and bifidobacterium bifidum) prevent nec in vlbw infants fed breast milk but not formula. | | 2016 | 26800318 |
| human gut-commensalic lactobacillus ruminis atcc 25644 displays sortase-assembled surface piliation: phenotypic characterization of its fimbrial operon through in silico predictive analysis and recombinant expression in lactococcus lactis. | sortase-dependent surface pili (or fimbriae) in gram-positive bacteria are well documented as a key virulence factor for certain harmful opportunistic pathogens. however, it is only recently known that these multi-subunit protein appendages are also belonging to the "friendly" commensals and now, with this new perspective, they have come to be categorized as a niche-adaptation factor as well. in this regard, it was shown earlier that sortase-assembled piliation is a native fixture of two human i ... | 2015 | 26709916 |
| clinical and metabolic response to probiotic administration in patients with major depressive disorder: a randomized, double-blind, placebo-controlled trial. | we are aware of no study examining the effects of probiotic supplementation on symptoms of depression, metabolic profiles, serum high-sensitivity c-reactive protein (hs-crp), and biomarkers of oxidative stress in patients with major depressive disorder (mdd). the present study was designed to determine the effects of probiotic intake on symptoms of depression and metabolic status in patients with mdd. | 2016 | 26706022 |
| three-dimensional tumor spheroids for in vitro analysis of bacteria as gene delivery vectors in tumor therapy. | several studies in animal models demonstrated that obligate and facultative anaerobic bacteria of the genera bifidobacterium, salmonella, or clostridium specifically colonize solid tumors. consequently, these and other bacteria are discussed as live vectors to deliver therapeutic genes to inhibit tumor growth. therapeutic approaches for cancer treatment using anaerobic bacteria have been investigated in different mouse models. in the present study, solid three-dimensional (3d) multicellular tumo ... | 2015 | 26655167 |
| exploring amino acid auxotrophy in bifidobacterium bifidum prl2010. | the acquisition and assimilation strategies followed by members of the infant gut microbiota to retrieve nitrogen from the gut lumen are still largely unknown. in particular, no information on these metabolic processes is available regarding bifidobacteria, which are among the first microbial colonizers of the human intestine. here, evaluation of amino acid auxotrophy and prototrophy of bifidobacterium bifidum, with particular emphasis on b. bifidum strain prl2010 (lmg s-28692), revealed a putat ... | 2015 | 26635786 |
| in vitro fermentation of caprine milk oligosaccharides by bifidobacteria isolated from breast-fed infants. | this study was conducted to investigate the catabolism and fermentation of caprine milk oligosaccharides (cmo) by selected bifidobacteria isolated from 4 breast-fed infants. seventeen bifidobacterial isolates consisting of 3 different species (bifidobacterium breve, bifidobacterium longum subsp. longum and bifidobacterium bifidum) were investigated. a cmo-enriched fraction (cmof) (50% oligosaccharides, 10% galacto-oligosaccharides (gos), 20% lactose, 10% glucose and 10% galactose) from caprine c ... | 2015 | 26587678 |
| probiotic dahi containing lactobacillus acidophilus and bifidobacterium bifidum modulates immunoglobulin levels and cytokines expression in whey proteins sensitised mice. | cow milk allergy is the most common food allergy in children. so far, no effective treatment is available to prevent or cure food allergy. this study investigated whether orally administrated probiotics could suppress sensitisation in whey proteins (wp)-induced allergy mouse model. two types of probiotic dahi were prepared by co-culturing dahi bacteria (lactococcus lactis ssp. cremoris ncdc-86 and lactococcus lactis ssp. lactis biovar diacetylactis ncdc-60) along with selected strain of lactobac ... | 2016 | 26459934 |
| colonization of c57bl/6 mice by a potential probiotic bifidobacterium bifidum strain under germ-free and specific pathogen-free conditions and during experimental colitis. | the effects of at least some probiotics are restricted to live, metabolically active bacteria at their site of action. colonization of and persistence in the gastrointestinal tract is thus contributing to the beneficial effects of these strains. in the present study, colonization of an anti-inflammatory bifidobacterium bifidum strain was studied in c57bl/6j mice under germ-free (gf) and specific pathogen-free (spf) conditions as well as during dextran sulfate sodium (dss)-induced colitis. b. bif ... | 2015 | 26439388 |
| amelioration of chemotherapy-induced intestinal mucositis by orally administered probiotics in a mouse model. | intestinal mucositis is a frequently encountered side effect in oncology patients undergoing chemotherapy. no well-established or up to date therapeutic strategies are available. to study a novel way to alleviate mucositis, we investigate the effects and safety of probiotic supplementation in ameliorating 5-fu-induced intestinal mucositis in a mouse model. | 2015 | 26406888 |
| [effect of probiotic product containing bifidobacteria and biogel from brown algae on the intestinal microflora and parameters of innate immunity in mice with experimental drug dysbacteriosis]. | the article represents the results of studying the effect of a new fermented product (fp) containing the probiotic strain bifidobacterium bifidum 791 and biogel from brown algae laminariajaponica on the composition of intestinal microflora and parameters of innate immunity in mice with experimental dysbacteriosis, induced by administration of gentamicin in dose of 25 mg per kg body weight during 7 days. the experimental animals received for 6 weeks in addition to the diet fp, which was 2% of the ... | 2015 | 26402946 |
| longitudinal study of effects of oral dosage of bifidobacterium bifidum g9-1 on japanese cedar pollen-induced allergic nasal symptoms in guinea pigs. | previous studies using experimental animal models have reported the beneficial effects of probiotics on allergic responses; however, their long-term effects on allergic nasal symptoms in clinical settings have not yet been elucidated in detail. in the present study, a guinea pig allergic rhinitis model involving repeated inhalation challenges with a natural allergen, japanese cedar pollen, was used to examine the longitudinal effects of bifidobacterium bifidum g9-1 (bbg9-1) on allergic nasal sym ... | 2015 | 26400839 |
| comparative transcriptomics reveals key differences in the response to milk oligosaccharides of infant gut-associated bifidobacteria. | breast milk enhances the predominance of bifidobacterium species in the infant gut, probably due to its large concentration of human milk oligosaccharides (hmo). here we screened infant-gut isolates of bifidobacterium longum subsp. infantis and bifidobacterium bifidum using individual hmo, and compared the global transcriptomes of representative isolates on major hmo by rna-seq. while b. infantis displayed homogeneous hmo-utilization patterns, b. bifidum were more diverse and some strains did no ... | 2015 | 26337101 |
| drying process strongly affects probiotics viability and functionalities. | probiotic formulations are widely used and are proposed to have a variety of beneficial effects, depending on the probiotic strains present in the product. the impact of drying processes on the viability of probiotics is well documented. however, the impact of these processes on probiotics functionality remains unclear. in this work, we investigated variations in seven different bacterial markers after various desiccation processes. markers were composed of four different viability evaluation (c ... | 2015 | 26325197 |
| insights into teichoic acid biosynthesis by bifidobacterium bifidum prl2010. | bifidobacteria are colonizers of the human gut, where they are interacting with their host as well as with other members of the intestinal microbiota. teichoic acids (tas) have previously been shown to play an important role in modulating microbe-host interactions in the human gut. however, so far, there is a paucity of information regarding the presence of tas in the cell envelope of bifidobacteria. in silico analyses targeting the chromosomes of all 48 (sub)species that currently represent the ... | 2015 | 26296705 |
| anti-amnesic effect of fermented ganoderma lucidum water extracts by lactic acid bacteria on scopolamine-induced memory impairment in rats. | this study investigated the anti-amnesic effect of fermented ganoderma lucidum water extracts (gw) on scopolamine-induced memory impairment in rats. gw were fermented by the lactic acid bacterium bifidobacterium bifidum (fgwb), followed by lactobacillus sakei li033 (fgwbl). to induce amnesia, scopolamine (1 mg/kg) was intraperitoneally injected into rats 30 min before the behavioral tests. step-through latencies of rats treated with primary fermented extracts (300 mg/kg, fgwb) and secondary ferm ... | 2015 | 26176000 |
| scalable temperature induced stress for the large-scale production of functionalized bifidobacteria. | the application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. in this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of bifidobacterium bifidum. however, this heat shock has been considered only in small-scale bioreactor and no information is available for a possible scaling-up strategy. five different operating scales (0.2 ... | 2015 | 26162630 |
| protective effect of probiotics in the treatment of infantile eczema. | the aim of the present study was to provide evidence for the application of probiotics in the prevention and treatment of infantile eczema by exploring changes in the intestinal bifidobacteria levels and the scoring atopic dermatitis (scorad) index prior and subsequent to treatment with probiotics in infants with eczema. a total of 40 infants with eczema were randomly divided into treatment and control groups. prior and subsequent to the treatment, the scorad index was evaluated and the content ... | 2015 | 26136864 |
| complete genome sequence of bifidobacterium bifidum jcm 1255(t) isolated from feces of a breast-fed infant. | bifidobacterium bifidum jcm 1255(t) was isolated from feces of a breast-fed infant. here we report the complete genome sequence of this organism. | 2015 | 26133927 |
| a phytase-based reporter system for identification of functional secretion signals in bifidobacteria. | health-promoting effects have been attributed to a number of bifidobacterium sp. strains. these effects as well as the ability to colonise the host depend on secreted proteins. moreover, rational design of protein secretion systems bears the potential for the generation of novel probiotic bifidobacteria with improved health-promoting or therapeutic properties. to date, there is only very limited data on secretion signals of bifidobacteria available. using in silico analysis, we demonstrate that ... | 2015 | 26086721 |
| structural-functional analysis reveals a specific domain organization in family gh20 hexosaminidases. | hexosaminidases are involved in important biological processes catalyzing the hydrolysis of n-acetyl-hexosaminyl residues in glycosaminoglycans and glycoconjugates. the gh20 enzymes present diverse domain organizations for which we propose two minimal model architectures: model a containing at least a non-catalytic gh20b domain and the catalytic one (gh20) always accompanied with an extra α-helix (gh20b-gh20-α), and model b with only the catalytic gh20 domain. the large bifidobacterium bifidum l ... | 2015 | 26024355 |
| a randomized double-blind controlled trial of lactobacillus acidophilus plus bifidobacterium bifidum versus placebo in patients with hypercholesterolemia. | hypercholesterolemia is a major risk factor for cardiovascular disease. not all patients respond well to traditional cholesterol lowering medications. probiotics have been evaluated for their cholesterol-lowering effects in humans with variable results. this study was performed to evaluate the efficacy of two probiotics in lowering the serum cholesterol of hypercholesterolemic patients. | 2015 | 25954637 |
| the role of intestinal epithelial barrier function in the development of nec. | the intestinal epithelial barrier plays an important role in maintaining host health. breakdown of intestinal barrier function is known to play a role in many diseases such as infectious enteritis, idiopathic inflammatory bowel disease, and neonatal inflammatory bowel diseases. recently, increasing research has demonstrated the importance of understanding how intestinal epithelial barrier function develops in the premature neonate in order to develop strategies to promote its maturation. optimiz ... | 2015 | 25927016 |
| continuous consumption of fermented milk containing bifidobacterium bifidum yit 10347 improves gastrointestinal and psychological symptoms in patients with functional gastrointestinal disorders. | the aim of this study was to investigate whether consumption of probiotic fermented milk containing bifidobacterium bifidum yit 10347 improves symptoms in patients with functional gastrointestinal disorders (fgid). thirty-seven fgid patients (18 male, 19 female) aged 12-80 years (mean ± sd, 52.6 ± 17.5 years) whose condition had not improved despite being seen at several medical institutions consumed 100 ml/day of b. bifidum yit 10347 fermented milk for 4 weeks. symptoms were evaluated after the ... | 2015 | 25918671 |
| lactobacillus gasseri ks-13, bifidobacterium bifidum g9-1, and bifidobacterium longum mm-2 ingestion induces a less inflammatory cytokine profile and a potentially beneficial shift in gut microbiota in older adults: a randomized, double-blind, placebo-controlled, crossover study. | this study determined whether older adults who consumed a probiotic mixture would have a greater proportion of circulating cd4+ lymphocytes, altered cytokine production, and a shift in intestinal microbiota toward a healthier microbial community. | 2015 | 25909149 |
| a study of the effect of dietary fiber fractions obtained from artichoke (cynara cardunculus l. var. scolymus) on the growth of intestinal bacteria associated with health. | the effect of different fractions enriched in soluble fiber obtained from artichoke using citric acid or citric acid/hemicellulase on the selective growth of lactobacillus plantarum 8114 and bifidobacterium bifidum atcc 11863 was evaluated. gompertz modeling of lactobacillus plantarum 8114 growth showed a higher specific growth rate (μ: 0.16 h(-1)) in the presence of fractions isolated from stems using hemicellulase (fraction a) than in the presence of glucose (μ: 0.09 h(-1)). in the case of bif ... | 2015 | 25904284 |
| the neonatal gut harbours distinct bifidobacterial strains. | recent studies have described the bifidobacterial composition of neonates at a species level; however, with advancing technologies we can gain insight into the diversity of the bifidobacterial microbiota residing within the infant gut. | 2015 | 25896967 |
| a novel lactobacillus casei lp1 producing 1,4-dihydroxy-2-naphthoic acid, a bifidogenic growth stimulator. | 1,4-dihydroxy-2-naphthoic acid (dhna) is a bifidogenic growth stimulator (bgs) and could be a functional food ingredient since bifidobacteria are beneficial for human health. for that reason, lactic acid bacteria producing dhna have been screened. a lactic acid bacterium lp1 strain isolated from a natural cheese was confirmed to produce dhna, analyzed by a hplc method. the strain was identified as lactobacillus casei by 16s rrna gene sequence analysis. the cell-free supernatant of fermented whey ... | 2015 | 25866754 |
| evidence for cholesterol-lowering activity by bifidobacterium bifidum prl2010 through gut microbiota modulation. | bifidobacteria are members of the human gut microbiota, which are known to influence the metabolic abilities of their host. here, we investigated the capabilities of bifidobacteria to reduce cholesterol levels in synthetic growth media, clearly demonstrating assimilation of this molecule by particular bifidobacterial strains, including bifidobacterium bifidum prl2010 (lmg s-28692). the transcriptomic analysis of prl2010 cells cultivated in the presence of cholesterol revealed a significantly inc ... | 2015 | 25863679 |
| a randomized controlled trial to test the effect of multispecies probiotics on cognitive reactivity to sad mood. | recent insights into the role of the human microbiota in cognitive and affective functioning have led to the hypothesis that probiotic supplementation may act as an adjuvant strategy to ameliorate or prevent depression. | 2015 | 25862297 |
| novel substrate specificities of two lacto-n-biosidases towards β-linked galacto-n-biose-containing oligosaccharides of globo h, gb5, and ga1. | we describe the novel substrate specificities of two independently evolved lacto-n-biosidases (lnbx and lnbb) towards the sugar chains of globo- and ganglio-series glycosphingolipids. lnbx, a non-classified member of the glycoside hydrolase family, isolated from bifidobacterium longum subsp. longum, was shown to liberate galacto-n-biose (gnb: galβ1-3galnac) and 2'-fucosyl gnb (a type-4 trisaccharide) from gb5 pentasaccharide and globo h hexasaccharide, respectively. lnbb, a member of the glycosi ... | 2015 | 25839135 |
| perioperative synbiotics decrease postoperative complications in periampullary neoplasms: a randomized, double-blind clinical trial. | periampullary neoplasms are rapidly progressive tumors with a poor prognosis and high morbidity and mortality rates, which have a negative influence on patient outcomes. some probiotics and prebiotics have the ability to protect the intestinal barrier and prevent bacterial translocation, infection, and postoperative complications. we evaluated the use of synbiotics in a prospective, double-blind study of patients undergoing surgery for periampullary neoplasms (pns) and assessed the effect of the ... | 2015 | 25803626 |
| strengthening of the intestinal epithelial tight junction by bifidobacterium bifidum. | epithelial barrier dysfunction has been implicated as one of the major contributors to the pathogenesis of inflammatory bowel disease. the increase in intestinal permeability allows the translocation of luminal antigens across the intestinal epithelium, leading to the exacerbation of colitis. thus, therapies targeted at specifically restoring tight junction barrier function are thought to have great potential as an alternative or supplement to immunology-based therapies. in this study, we screen ... | 2015 | 25780093 |
| [changes in splenocyte proliferation, subsets and cytokine production in mice immunized with recombinant vaccine bifidobacterium bifidum (pgex-sj32) of schistosoma japonicum]. | to observe the dynamic changes of immune responses of splenocytes in mice immunized with recombinant vaccine bifidobacterium bifidum (pgex-sj32) of schistosoma japonicum and investigate the immunological mechanism of the vaccine. | 2015 | 25736113 |
| interactions of salmonella enterica subspecies enterica serovar typhimurium with gut bacteria. | the aim of this study was to evaluate the impact of the gut microbiota on the growth and survival of s. typhimurium. this was tested in two-species co-cultures and in mixed cultures with a simplified gut model microbiota. subsequently, interactions between s. typhimurium and human faecal bacteria were quantified in both batch and continuous culture systems simulating the human colon. the exponential growth of s. typhimurium was halted when the population of escherichia coli reached the maximum p ... | 2015 | 25727571 |
| [biological effect of extracellular peptide factor from lateococcus japonicas subsp. casei on probiotic bacteria]. | the biological effect of the extracellular peptide reactivating factor (rf) from luteococcus casei on cells of probiotic cultures was studied. the rf showed the protective and reactivating effects on the bifidobacterium bifidum cells under the action of bile salts and an acidic stress. also, it acted as a cryoprotector during lyophilisation and long-term culture storage. the rf and the l. casei culture liquid (cl) were shown to have bifidogenic properties. the degree of protection and reactivati ... | 2015 | 25707114 |
| multilocus sequence typing of bifidobacterial strains from infant's faeces and human milk: are bifidobacteria being sustainably shared during breastfeeding? | bifidobacteria are considered to be one of the most important beneficial intestinal bacteria for infants, contributing to the priming of the mucosal immune system. these microbes can also be detected in mother's milk, suggesting a potential role of human milk in the colonisation of infant's gut. however, little is known about the timing of bacteria appearance in human milk, and whether human milk is the first source of inoculation. here, we investigated whether specific strains are shared sustai ... | 2015 | 25691099 |
| health benefits of fermented milk containing bifidobacterium bifidum yit 10347 on gastric symptoms in adults. | we conducted a preliminary open trial (trial 1) and a double-blind, placebo-controlled, crossover trial (trial 2) to examine how fermented milk containing the probiotic bifidobacterium bifidum yit 10347 affects gastric and lower abdominal symptoms in adults taking no medication. in trial 1, subjects with or without gastric and lower abdominal symptoms ingested fermented milk containing b. bifidum yit 10347 daily for 2 wk. in trial 2, subjects with gastric symptoms ingested fermented milk contain ... | 2015 | 25648808 |
| determination of microbiological and chemical properties of probiotic boza and its consumer acceptability. | the aim of research was producing boza as a probiotic product with certificated probiotic starter cultures (lactobacillus acidophilus la-5 and bifidobacterium bifidum bb-12 and saccharomyces boulardii). | 2015 | 25648272 |
| factors affecting viability of bifidobacterium bifidum during spray drying. | there is substantial clinical data supporting the role of bifidobacterium bifidum in human health particularly in benefiting the immune system and suppressing intestinal infections. compared to the traditional lyophilization, spray-drying is an economical process for preparing large quantities of viable microorganisms. the technique offers high production rates and low operating costs but is not usually used for drying of substances prone to high temperature. the aim of this study was to establi ... | 2015 | 25618319 |
| bifidobacterium bifidum r0071 results in a greater proportion of healthy days and a lower percentage of academically stressed students reporting a day of cold/flu: a randomised, double-blind, placebo-controlled study. | acute psychological stress is positively associated with a cold/flu. the present randomised, double-blind, placebo-controlled study examined the effect of three potentially probiotic bacteria on the proportion of healthy days over a 6-week period in academically stressed undergraduate students (n 581) who received lactobacillus helveticus r0052, bifidobacterium longum ssp. infantis r0033, bifidobacterium bifidum r0071 or placebo. on each day, participants recorded the intensity (scale: 0 = not e ... | 2015 | 25604727 |
| bioactivity of water soluble extracts and some characteristics of white cheese during the ripening period as effected by packaging type and probiotic adjunct cultures. | in this study, the chemical composition, proteolysis and in vitro angiotensin-converting enzyme-(ace)-inhibitory and antioxidant activities of white cheeses made using probiotic adjunct cultures (bifidobacterium bifidum dsmz 20456 and lactobacillus acidophilus dsmz 20079) were investigated. the cheeses were ripened in a vacuum package or brine for 120 d at 4 °c. the cheese samples maintained the probiotic characteristics of the viable cells as >106 cfu/g even after ripening for 120 d. the proteo ... | 2015 | 25592630 |
| effect of bifidobacterium bifidum containing yoghurt on dental plaque bacteria in children. | the aim of the present study is to determine the possible effect of bifidobacterium bifidum dn-173 010 on dental plaque of children. | 2014 | 25571684 |
| probiotics for the primary and secondary prevention of c. difficile infections: a meta-analysis and systematic review. | clostridium difficile infections are a global clinical concern and are one of the leading causes of nosocomial outbreaks. preventing these infections has benefited from multidisciplinary infection control strategies and new antibiotics, but the problem persists. probiotics are effective in preventing antibiotic-associated diarrhea and may also be a beneficial strategy for c. difficile infections, but randomized controlled trials are scarce. this meta-analysis pools 21 randomized, controlled tria ... | 2015 | 27025619 |
| comparison of mupirocin-based media for selective enumeration of bifidobacteria in probiotic supplements. | an international standard already exists for the selective enumeration of bifidobacteria in milk products. this standard uses transgalactosylated oligosaccharides (tos) propionate agar supplemented with mupirocin. however, no such standard method has been described for the selective enumeration of bifidobacteria in probiotic supplements, where the presence of bifidobacteria is much more variable than in milk products. therefore, we enumerated bifidobacteria by colony count technique in 13 probio ... | 2015 | 25542994 |