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isolation of serratia, arcanobacterium and burkholderia species from visceral and cutaneous abscesses in four emaciated ewes. 200415508845
inflammation in the bovine female reproductive tract.inflammation of the reproductive tract of a cow occurs when the physical and functional barriers to contamination are breached or specific infection occurs. commonly, contamination occurs at parturition and to a lesser extent at estrus. uterine contamination following calving is common, but most healthy cows are able to clear the uterus of bacteria in the first 2 to 3 wk after calving. persistent infections are more likely to be caused by actinomyces pyogenes. specific venereal infections tend t ...199915526785
a retrospective study of mortality in pennsylvania captive white-tailed deer (odocoileus virginianus): 20000--2003.the postmortem records of 160 white-tailed deer (odocoileus virginianus) submitted for necropsy examination from 59 separate pennsylvania captive deer farms over a 3.5-year period were reviewed to determine the primary cause of death of each animal. the most common causes of death were bronchopneumonia (39 cases), enterocolitis (30 cases), malnutrition (13 cases), and trauma (11 cases). other causes of mortality included severe gastrointestinal parasitism (6 cases), cellulitis with septicemia (5 ...200415586566
clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle.bacteria contaminate the uterus of most dairy cattle after parturition and endometritis causes infertility. an endometritis score can be ascribed based on the vaginal mucus character and odour but it is not clear if the clinical score reflects the number of uterine bacteria or the inflammatory response. the present study tested the hypothesis that clinical evaluation of endometritis reflects the number of bacteria present in the uterus, and the acute phase protein response. swabs (n = 328) were ...200515589277
a case series on chronic canaliculitis.primary chronic canaliculitis is an uncommon disease, which is often misdiagnosed and insufficiently treated. we present two cases of canaliculitis caused by two different organisms, actinomycetes spp. and arcanobacterium haemolyticum. to the best of our knowledge, canaliculitis due to arcanobacterium haemolyticum has not been reported before.200515764110
[bacterially conditioned thromboembolism in dairy cows--a retrospective study of 31 necropsy cases with special consideration of the causative complex].a study of 340 necropsied dairy cattle from northern bavaria resulted in 31 animals (9%) showing evidence of pyemic thromboembolism. the most frequent pathomorphologic lesions consisted in endocarditis valvularis thromboticans of the tricuspid and/or mitral valve (21), embolic pneumonia (26), nephritis (13) and renal infarction (12). the most common isolate found in bacteriologic culture was arcanobacterium pyogenes in 26 of 31 cases, followed by staphylococcus aureus (2), mannheimia haemolytica ...200515803759
enzootic pneumonia of pigs--a diagnostic dilemma.in an enzootic pneumonia-free australian pig herd, an outbreak of a severe respiratory disease in the grow-out herd was initially diagnosed as acute tracheitis and pneumonia precipitated by the dusty environment, with a superimposed mixed infection of pasteurella multocida and arcanobacterium pyogenes. culture for mycoplasma hyopneumoniae, salmonella sp and fungi was negative. the outbreak persisted. subsequently, gross lesions consistent with enzootic pneumonia occurred, but histological lesion ...200515825621
bovine herpesvirus 4-associated postpartum metritis in a spanish dairy herd.in more than 10 spanish dairy cows, a bovine herpesvirus 4 (bhv4) associated postpartum metritis was confirmed by virus isolation, bhv4-glycoprotein b (gb) pcr and/or serology. in this study, 12 cows with, and, at the time of sampling, 3 cows without clinical signs of acute postpartum metritis from one large dairy herd in spain were examined for bacterial and viral infections. blood, placenta/caruncles and uterine contents were collected between day 1 and day 20 post-calving, and examined for th ...200615946713
pulmonary infections in swine induce altered porcine surfactant protein d expression and localization to dendritic cells in bronchial-associated lymphoid tissue.surfactant protein d (sp-d) is a pattern-recognition molecule of the innate immune system that recognizes various microbial surface-specific carbohydrate and lipid patterns. in vitro data has suggested that this binding may lead to increased microbial association with macrophages and dendritic cells. the aim of the present in vivo study was to study the expression of porcine sp-d (psp-d) in the lung during different pulmonary bacterial infections, and the effect of the routes of infection on thi ...200516011521
infectious epiphysitis and valvular endocarditis in a red deer (cervus elaphus).a 10-month-old male red deer (cervus elaphus) was presented with severe lameness and enlargement of the fetlock areas of both front and one hind legs. radiographs demonstrated an epiphysitis and osteomyelitis of the distal metacarpal and metatarsal bones. post mortem revealed pus containing corynebacterium pyogenes and staphylococcus aureus in the lesions, and an accompanying valvular endocarditis of the right atrioventricular valve.198216030849
arcanobacterium pyogenes mastitis in a 18-month-old heifer. 200216032265
gross abnormalities, bacteriology and histological lesions of uteri of dairy cows failing to conceive or maintain pregnancy.to describe the gross pathology, bacteriology and histopathology of the reproductive tracts of dairy cows that failed to conceive or maintain pregnancy.200516044186
arcanobacterium pyogenes: molecular pathogenesis of an animal opportunist.arcanobacterium pyogenes is a commensal and an opportunistic pathogen of economically important livestock, causing diseases as diverse as mastitis, liver abscessation and pneumonia. this organism possesses a number of virulence factors that contribute to its pathogenic potential. a. pyogenes expresses a cholesterol-dependent cytolysin, pyolysin, which is a haemolysin and is cytolytic for immune cells, including macrophages. expression of pyolysin is required for virulence and this molecule is th ...200516096685
effect of pathogen-specific clinical mastitis on herd life in two new york state dairy herds.the objective of this study was to estimate the effects of clinical mastitis (cm) (both with and without specific pathogen identification) occurring in different stages of lactation on length of herd life in two new york state dairy farms. the 2,697 cows in the study were followed for one lactation (the first-occurring one on or after 1 october 1999), until it ended because of a new lactation, culling, or end of study (31 march 2001 in one farm; 31 july 2001 in the other). a cox proportional haz ...200516111778
prevalence of aerobic and anaerobic uterine bacteria during peripartum period in normal and dystocia-affected buffaloes.parturition complications predispose establishment of uterine infections, which in turn affect subsequent fertility. the aim of present study was to characterize and compare the type of bacterial flora prevalent within the uterine lumen of dystocia-affected buffaloes and compare them with the normally calving buffaloes. the study was conducted on 40 buffaloes; of which 10 calved normally (group i) and 30 were treated for dystocia (group ii). bacteriological examination was performed using uterin ...200516143213
locomotive disorders associated with sow mortality in danish pig herds.given the increase in sow mortality in danish pig herds over the last 10 years, we have investigated the causes of spontaneous death and killing of sows, especially those due to locomotive disorders. a total of 265 culled sows (93 spontaneously dead sows and 172 killed sows) from 10 different herds were sent for complete necropsy, and grouped according to primary diagnoses. from 35 of these sows, tissue for histological evaluation and samples for bacteriological cultivation were also collected, ...200516176574
[resistance of mastitis pathogens in northern germany].the data of 1692 susceptibility tests acquired from april 2003 through march 2004 in the mastitis laboratory of the institute for food quality and safety were summarized in order to help veterinarians confronted with acute mastitis in choosing the appropriate antibiotic. two thirds of the milk samples were infected with gram-positive cocci. one third of these were identified as streptococcus (s.) uberis, one fourth as staphylococcus (s.) aureus. all isolates (100%) of s. uberis, s. dysgalactiae, ...200516206927
uterine response to multiple inoculations with arcanobacterium pyogenes and escherichia coli in nulliparous ewes.uterine infections seem more severe in nulliparous animals. our objective was to determine whether intrauterine inoculation of nulliparous ewes with arcanobacterium pyogenes and escherichia coli would produce an antibody response and reduce the severity of subsequent infections.200516212647
perinatal exposure to low doses of pcb 153 and pcb 126 affects maternal and neonatal immunity in goat kids.pregnant does (10 goats/group) were dosed orally either with polychlorinated biphenyl (pcb) 153 (98 microg/kg body weight/d) or pcb 126 (ng/kg body weight/d) dissolved in corn oil or with corn oil only (control group) from gestation day (gd) 60 until delivery. an additional group (n = 5) of pregnant does received the synthetic estrogen diethylstilbestrol (des; 0.4 microg/kg body weight/d) by intramuscular injection using the same treatment schedule as for the pcb groups. blood samples for immune ...200616291567
slaughterhouse survey of pyelonephritis in dairy cows.twenty-one rejected kidneys from 2426 slaughtered dairy cows (0.87 per cent) had gross signs of pyelonephritis that were confirmed by histopathology. in all the kidneys the findings were consistent with a chronic rather than an acute infection. one species of bacteria was cultured from 12 of the kidneys and two species of bacteria were cultured from six. the most commonly isolated bacteria were escherichia coli, from eight kidneys, arcanobacterium pyogenes, from seven kidneys and corynebacterium ...200516299366
bacteriological findings and hormonal profiles in the postpartum balady goats.twenty-six balady goats categorized according to parity into primiparous and pluriparous goats were used to investigate bacterial flora of the genital tract and hormonal profiles during the postpartum (pp) period. escherichia coli and staphylococcus aureus were isolated in pure or mixed culture from the uterus. arcanobacterium pyogenes was isolated from swabs obtained from the vagina and cervix of one primiparous goat. uteri and cervices but not vaginas were free of bacterial contamination by da ...200616420321
diagnostic ultrasonography in cattle with diseases of the mammary gland.fifty-two cattle with diseases of the udder were evaluated by means of sonography between 2000 and 2004. the udders were examined with 3.5 and 5.0 mhz convex transducers and 7.5 and 13.0 mhz linear transducers. bacteriological examinations were also performed on individual quarter samples. ultrasonography gave additional information on the status of the udder and showed findings specific for some causal agents, e.g., enterobacteriaceae and arcanobacterium pyogenes as well as udder haematomas. al ...200616490715
diseases and pathogens associated with mortality in ontario beef feedlots.this study determined the prevalence of diseases and pathogens associated with mortality or severe morbidity in 72 ontario beef feedlots in calves that died or were euthanized within 60 days after arrival. routine pathologic and microbiologic investigations, as well as immunohistochemical staining for detection of bovine viral diarrhea virus (bvdv) antigen, were performed on 99 calves that died or were euthanized within 60 days after arrival. major disease conditions identified included fibrinos ...200616566254
arcanobacterium bialowiezense sp. nov. and arcanobacterium bonasi sp. nov., isolated from the prepuce of european bison bulls (bison bonasus) suffering from balanoposthitis, and emended description of the genus arcanobacterium collins et al. 1983.a taxonomic study was performed on 13 bacterial strains isolated from preputial swabs of european bison (bison bonasus) bulls suffering from balanoposthitis. the isolates were gram-positive, non-motile, facultatively anaerobic, diphtheroid-shaped cells. based on biochemical profiles and box-pcr-generated genomic fingerprints, the isolates were grouped into two clusters represented by four and nine strains, respectively. strains 1(w3/01)t and 2(w106/04)t, selected as representatives of the two cl ...200616585707
ulcerative balanitis and vulvitis of dorper sheep in south africa: a study on its aetiology and clinical features.ovine ulcerative balanitis and vulvitis in sheep of the dorper breed has been observed in south africa since 1979. its aetiology has not been conclusively resolved, and there is some discrepancy in descriptions of its clinical features. in order to identify the pathogenic micro-organism/s that contribute to the occurrence of the disease, the microflora in the genital tracts of both clinically healthy and affected sheep were isolated and compared. bacteriological examination of materials from aff ...200516642715
in vitro antimicrobial susceptibility of mycoplasma mycoides mycoides large colony and arcanobacterium pyogenes isolated from clinical cases of ulcerative balanitis and vulvitis in dorper sheep in south africa.the in vitro activities of enrofloxacin, florfenicol, oxytetracycline and spiramycin were determined against field isolates of mycoplasma mycoides mycoides large colony (mmmlc) by means of the broth microdilution technique. the minimum inhibitory concentrations (mics) of these antimicrobial drugs were determined for a representative number of 10 isolates and 1 type strain. the susceptibility of arcanobacterium pyogenes to enrofloxacin, oxytetracycline and tilmicosin was determined by means of an ...200516642716
effect of infusion of uterine purulent exudate into the bovine uterus.purulent exudate from the uteri of two nonlactating holstein cows with chronic pyometra was aspirated into a 50 cc syringe via an artificial insemination pipette. the two fractions were mixed, cultured, divided into 14 equal parts and infused into the uteri of 14 other nonlactating cows. all recipient cows had had at least one estrous cycle of normal length and were in the early luteal phase (day 4-8). on days 3, 7, 14 and 21 following infusion, the reproductive tract was examined per rectum, co ...198016725499
successful transfer of embryos recovered from a cow with chronic cervicitis of mixed aerobic and anaerobic bacterial aetiology a case report.an imported 13-year-old simmentaler cow was presented with severe purulent cervicitis and endometritis from which corynebacterium pyogenes , fusobacterium necrophorum , bacteroides melaninogenicus and an untyped clostridium perfringens were isolated. the endometritis responded to treatment but the cervix did not and remained fibrous, continuously patent and purulent. natural pregnancy was considered unlikely and the cow was prepared as an embryo transfer donor. eight embryos were recovered and s ...198516726028
ultrastructural assessment of a blastocyst recovered from a cow with subclinical purulent endometritis.a blastocyst was recovered from a cow with subclinical purulent endometritis from which actinomyces pyogenes was isolated. ultrastructurally the blastocyst appeared viable, although the zona pellucida and pervitelline cell debris were undergoing phagocytosis by neutrophils. an increase in lysosomal activity was observed in the inner cell mass, which may represent sublethal injury due to inflammatory alteration of the uterine milieu. it was concluded that the embryo was resistant to the bystander ...198816726388
retained fetal membranes in cows: manual removal versus nonremoval and its effect on reproductive performance.sixteen primiparous holstein cows with retained fetal membranes (rfm) were studied for postpartum prostaglandin release, uterine infection and resumption of estrous cyclicity after manual removal of rfm (eight cows) versus leaving the rfm untreated (eight cows). the rfm were results of induced parturition on day 274 of gestation. seventeen non-rfm primiparous cows were controls. the 15-keto-13, 14-dihydro-metabolite of prostaglandin f(2alpha) (pgfm) was measured in daily blood samples. aerobic a ...198816726448
the effects of ovarian hormones and acth on uterine defense to corynebacterium pyogenes in cows.ovariectomized cows were treated with either estrogen, progesterone or adrenogorticotropic hormone (acth). each cow's uterus was inoculated with 1x10(6)corynebacterium pyogenes organisms. the cows were evaluated by rectal examination for signs of uterine infection. peripheral leukocytic phagocytosis was determined by chemiluminescence at the beginning (day 4) and end (day 21) of the experiment. uterine cultures were obtained at the end. none of the estrogen-treated cows developed signs of uterin ...198816726452
effect of actinomyces pyogenes and gram-negative anaerobic bacteria on the development of bovine pyometra.fifteen lactating holstein cows were used in a trial designed to evaluate the effectiveness of intrauterine inoculation (challenge) of actinomyces pyogenes (a) alone or in combination with fusobacterium necrophorum (f) and bacteroides melaninogenicus (b) to induce pyometra. cows were assigned to one of five groups: a (n = 3), ab (n = 3), af (n = 3), abf (n = 3) or c (control, broth medium alone; n = 3). all cows exhibited estrus 12 or 13 d prior to challenge (day 0=first day of challenge). durin ...198916726615
bacterial contamination of the uterus after parturition and its effect on the reproductive performance of cows on large-scale dairy farms.the bacteriological status of the uteri of 150 cows was examined on 14 large-scale dairy farms 10 to 20 d after parturition and then twice again in 2-wk intervals during uterine involution. the degree of bacterial contamination and the proportion of the infected uterus as well as the composition of the isolated flora were determined. the antibiotic sensitivity of the important bacterium strains was also studied. the relationship between the reproductive data and bacterial contamination of the ut ...199016726782
the effect of prostaglandin administration to holstein-friesian cows at day 26 postpartum on clinical findings, and histological and bacteriological results of endometrial biopsies at day 40.ninety-two cows that calved between april and august 1984 in a commercial dairy herd were studied. the cows were randomly assigned to receive either cloprostenol (500 mug) or saline placebo on day 26 postpartum. in addition, each cow was examined per rectum, had three endometrial biopsies taken and milk progesterone level determined. double-blind techniques were used in administering treatments and in assessing response to treatment. the results of histological and bacteriological assessment of ...199016726784
pregnancy-specific protein b and progesterone in monitoring viability of the embryo in early pregnancy in the cow after experimental infection with actinomyces pyogenes.actinomyces pyogenes can cause embryonic death and abortion during the early stages of pregnancy in cows. bovine pregnancy-specific protein b (pspb) is produced in response to a viable embryo and as such it could be a potential marker for embryronic survival. the plasma concentration of pspb was monitored in cows following an intrauterine infection with a. pyogenes and during the subsequent abortion and recovery from infection. plasma progesterone concentrations were also monitored, and the resu ...199216727075
spontaneous uterine infections are associated with elevated prostaglandin f(2)alpha metabolite concentrations in postpartum dairy cows.postpartum holstein (n=21) and jersey (n=4) cows were used to determine if uterine infections are associated with elevated plasma concentrations of 13,14-dihydro-15-keto-prostaglandin f(2)alpha (pgfm). based upon clinical examinations and bacterial content of intrauterine fluid samples, cows detected with uterine infections between 21 and 28 d post partum were used (infected; n=14). these cows were matched with herdmates that were free of infection (control; n=11). beginning on the day the cows ...199416727399
effect of induced pyometra on luteal lifespan and uterine fluid concentrations of prostaglandins and interferons in cows.the objective was to determine whether occurrence of persistent corpora lutea in cows with pyometra could be explained by accumulation of prostaglandins and interferons in the uterus. pyometra was induced by treatment with hcg, followed by intrauterine infusions of iodine and actinomyces pyogenes . five of the seven treated cows developed pyometra as indicated by extended estrous cycles (30-37 + d) and presence of purulent uterine exudate at slaughter (day 37 after estrus). mean (+/- standard er ...199616727809
neutrophil migration into the uterine lumen of the cow: the influence of endogenous and exogenous sex steroid hormones using two intrauterine chemoattractants.neutrophil migration into the uterine lumen in response to the intrauterine infusion of both an oyster glycogen suspension and a bacteria-free filtrate obtained after incubation of actinomyces pyogenes in cooked meat medium was studied in 4 cows at estrus (day 0) and at diestrus (day 10). in addition, the same chemoattractants were used in 5 other cows following bilateral ovariectomy and after parenteral treatment with exogenous estradiol 17beta and progesterone in oil. large numbers of cells (a ...199716728032
effect of retained placenta on subsequent bacteriological and cytological intrauterine environment and reproduction in holstein dairy cows.to determine the effect of retained placenta on the characteristics of the intrauterine environment in dairy cows, bacteriological and cytological tests were performed on intrauterine perfusion fluid. the rate of cows with more than 70% neutrophils or fewer than 40% lymphocytes in inflammatory cells was 48.0% (12/25), while the rate and with more than 50 bacterial colonies/0.1 ml of perfusate was 96.0% (24/25) at 30 d after parturition. actinomyces pyogenes was isolated from 56.0% (14/25). at 60 ...199716728157
carcass condemnation causes of growth retarded pigs at slaughter.condemnation causes of growth retarded pigs were studied in a spanish abattoir. a total of 513 carcasses out of 6017 (8.5%) were rejected during inspection. the main reasons for condemnation were abscesses, cachexia, catarrhal bronchopneumonia, vertebral osteomyelitis, arthritis, pleuritis, peritonitis and pleuropneumonia. positive relationships were found between tail lesions and arthritis (or=5.23) or vertebral osteomyelitis (or=24.81), while no relationships were found between tail lesions an ...200716807012
effect of treatment with two intravaginal inserts on the uterine and vaginal microflora of early postpartum beef cows.to describe the bacterial species found within the uterus and vagina of early postpartum (10 to 20 d) beef cows treated for 14 d with (i) two intravaginal (cidr-b) progesterone releasing inserts (c-p4; n=31); (ii) two identical but blank inserts (c-bl; n=15); (iii) untreated controls (con; n=15). it was hypothesised that due to the locally immunosuppressive effects of progesterone on the uterus, the bacterial microflora of c-p4 would be altered by this treatment in contrast to con and c-bl.200616821488
probiotic properties of vaginal lactic acid bacteria to prevent metritis in cattle.the isolation of bovine vaginal lactic acid bacteria (lab) and the screening of their beneficial properties to select those that could be used as probiotics in the prevention of bovine metritis were performed.200616834727
prostaglandin f2alpha upregulates uterine immune defenses in the presence of the immunosuppressive steroid progesterone.uterine infections often develop in some livestock species during the first luteal phase postpartum. exogenous prostaglandin f(2alpha) (pgf(2alpha)) induces luteolysis, reduces progesterone, and enables the uterus to resolve infections. however, the effects of pgf(2alpha) on luteal function and on immune functions are confounded. these effects must be disentangled to determine whether alternatives to antibiotic treatments can be successfully developed.200616836612
ceftiofur derivatives in serum, uterine tissues, cotyledons, and lochia after fetal membrane retention.the objective of the study was to determine concentrations of ceftiofur derivatives after subcutaneous application of ceftiofur hydrochloride in cows with retained fetal membranes. concentrations of ceftiofur derivatives detected as desfuroylceftiofuracetamide were determined in blood serum, endometrium, caruncles, cotyledons, and lochia during 72 h. after induction of parturition, 2 primiparous and 4 multiparous cows having retained fetal membranes for at least 12 h were studied. all cows recei ...200616899676
multiple genetic elements carry the tetracycline resistance gene tet(w) in the animal pathogen arcanobacterium pyogenes.the tet(w) gene is associated with tetracycline resistance in a wide range of bacterial species, including obligately anaerobic rumen bacteria and isolates from the human gut and oral mucosa. however, little is known about how this gene is disseminated and the types of genetic elements it is carried on. we examined tetracycline-resistant isolates of the animal commensal and opportunistic pathogen arcanobacterium pyogenes, all of which carried tet(w), and identified three genetic elements designa ...200616966401
synovial fluid analysis and bacterial findings in arthritic joints of juvenile male camel (camelus dromedarius) calves.eighteen synovial fluid samples from 11 male dromedarian calves, 9-12 month old, were analysed cytologically and bacteriologically. calves were lame and all joints were grossly swollen. the mean +/- sd of total nucleated cell count was 7970 +/- 5000 cells/microl (range 2800-20,000 cells/microl). polymorphonuclear (pmn) leucocytes were the predominant cell type. the mean +/- sd of absolute and percentages of each cell type were as follows: pmn leucocytes 5518 +/- 3600 cells/microl and 68 +/- 19%, ...200717305968
morbidity and mortality associated with arcanobacterium pyogenes in a group of captive blackbuck (antilope cervicapra).arcanobacterium pyogenes was associated with necrotizing pneumonia; mandibular osteomyelitis; peritonitis; and hepatic, pulmonary, renal, and subcutaneous abscessation in a group of captive blackbuck (antilope cervicapra). males were more frequently (73.3%) affected than females. infection with a. pyogenes was fatal or necessitated euthanasia in 15 of 16 (93.7%) cases. deaths associated with a. pyogenes occurred most frequently (60%) during winter.200517323570
intraglandular injection of antibiotics for the treatment of vesicular adenitis in bulls.two experiments were designed to determine the efficacy of intraglandular antibiotic treatment in beef bulls. experiment 1 was designed to evaluate the glandular tissue reaction to intraglandular antibiotic treatment. experiment 2 was conducted to determine the efficacy intraglandular injection of antibiotics for the treatment of naturally occurring cases of vesicular adenitis. healthy beef bulls (n=15), 2 and 3 years of age, were randomly allocated to three equal treatment groups to receive 10% ...200817399921
an epizootic of swine influenza in ontario.a swine influenza virus (h(1)n(1)) was isolated for the first time in ontario from pigs one week to one and one-half years old during an epizootic which occurred between january and may 1981. each herd outbreak was characterized by the sudden onset of marked respiratory distress, usually affecting the entire herd, accompanied by paroxysmal coughing, anorexia, prostration and temperatures as high as 41.5 degrees c and lasting for five to seven days. morbidity was nearly 100%; mortality was less t ...198317422266
observations on intramammary infections in first calf heifers in early lactation.intramammary infections and mastitis were monitored on four occasions at three-week intervals in 61 first calf heifers in five dairy herds during the first ten weeks of lactation. of 940 quarter milk samples examined 65% were classed as negative, 10.4% as positive for mastitis (mainly subclinical), 1.8% as group 3 (infection present but no elevation in somatic cell count) and 22.8% as group 4 (elevated somatic cell count). seventy-seven percent of the infections detected and identified were thos ...198617422635
mortality in pennsylvania captive elk (cervus elaphus): 1998-2006.the necropsy records of 65 elk ranging from 1 day to 13(1/2) years of age were reviewed to determine the primary cause of death of each animal. the animal carcasses were submitted from 22 separate pennsylvania elk farms over an approximately 8-year period. the most common causes of mortality in animals in which the cause of death was determined were gastrointestinal parasitism (21 cases), pneumonia (7 cases), enterocolitis (5 cases), malnutrition (5 cases), and rumenitis/acidosis (5 cases). the ...200717459871
possible origins of ctnbst, a conjugative transposon found recently in a human colonic bacteroides strain.a previous survey of bacteroides isolates suggested that the ermb gene entered bacteroides spp. recently. previously, ermb had been found almost exclusively in gram-positive bacteria. in one bacteroides strain, ermb was located on 100-kb conjugative transposon (ctn) ctnbst. to assess the possible origin of this ctn, we obtained the full dna sequence of ctnbst and used this information to investigate its possible origins. over one-half of ctnbst had high sequence identity to a putative ctn found ...200717483268
hemorrhagic necrotizing splenitis in a slaughter pig infected with arcanobacterium species.a 6-month-old barrow presented with lethargy, inappetence and dysstasia. at necropsy, multiple coalescing hemorrhagic foci were detected in the margins of the spleen. gram-positive bacilli were isolated from the spleen, kidney, muscle and liver. comparative 16s rdna gene sequencing analysis of the isolates (to16177) revealed that they would be the same species of unpublished arcanobacterium species strain hj57-14e (accession no. gi 18873551) (99.7% similarity based on a comparison of 675 bp). hi ...200717485940
protostrongylid parasites and pneumonia in captive and wild thinhorn sheep (ovis dalli).we describe health significance of protostrongylid parasites (parelaphostrongylus odocoilei and protostrongylus stilesi) and other respiratory pathogens in more than 50 naturally infected dall's sheep (ovis dalli dalli) from the mackenzie mountains, northwest territories (1998-2002) as well as in three stone's sheep (o. d. stonei) experimentally infected with p. odocoilei (2000-2002). histological lesions in the brain and distribution of p. odocoilei in the muscles of experimentally and naturall ...200717495303
an investigation of the aetiological role of bovine herpesvirus 4 in bovine endometritis.uteri from 31 infertile cattle were examined for the presence of bovine herpesvirus 4 (bohv-4) by nested polymerase chain reaction (pcr). samples were also tested for bacteria, including chlamydiae and mycoplasma bovis. bohv-4 was detected by pcr in 27/31 (87.1%) samples, but the presence and amount of viral dna was not correlated with histological and bacteriological findings. arcanobacterium pyogenes, histophilus somni and pasteurella multocida were isolated from five cows with endometritis. c ...200817572123
a fatal case of arcanobacterium pyogenes endocarditis in a man with no identified animal contact: case report and review of the literature.arcanobacterium pyogenes is a rare cause of infection in humans, mostly related to living in rural areas and to contacts with animals. we describe a case of fatal arcanobacterium pyogenes endocarditis, confirmed by dna sequencing, in a patient without typical epidemiological exposure.200717610093
tongue lesions in cattle apparently caused by corynebacterium pyogenes. 195217648613
liver abscesses in holstein dairy cattle: 18 cases (1992-2003).the characteristic clinical manifestations, clinicopathologic findings, treatment, and outcome of dairy cattle with liver abscess are poorly defined.200717708409
intrauterine bacterial inoculation and level of dietary methionine alter amino acid metabolism in nulliparous yearling ewes.using an intrauterine bacterial inoculation method, our objective was to determine the effects of acute sepsis and level of dietary metabolizable met (mm) on splanchnic metabolism of aa in ewes. twenty-four nulliparous yearling rambouillet-cross ewes (initial bw = 65.1 +/- 0.6 kg), surgically fitted with chronic-indwelling catheters in hepatic and portal veins, a mesenteric vein and artery, and the uterine lumen, were assigned to a 2 x 2 factorial arrangement of treatments. factors were intraute ...200717785602
the effects of arcanobacterium pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo.uterine bacterial infection after parturition causes endometritis, perturbs ovarian function and leads to infertility in cattle. although endometritis is caused by mixed infections, endometrial pathology is associated with the presence of arcanobacterium pyogenes. the aims of the present study were to determine the effects of a. pyogenes on endometrial function in vitro, and on uterine and ovarian function in vivo. heat-killed a. pyogenes did not affect the production of prostaglandin f2alpha (p ...200717825901
clinical, bacteriological, and histopathological study of toxic puerperal metritis in iraqi buffalo.data were collected from 42 buffalo with toxic puerperal metritis in 2 large herds, with a history of dystocia, prolapse, and retained placenta. all buffalo were subjected to detailed clinical examination including external inspection, vaginoscopy, and transrectal palpation of the cervix, uterus, and ovaries. swabs for bacteriology and biopsies for histopathology were collected from the uterine lumen from each cow. character, odor, and estimation of polymorphonuclear cells of the vaginal mucus w ...200717881686
functional and structural properties of cbpa, a collagen-binding protein from arcanobacterium pyogenes.arcanobacterium pyogenes, an opportunistic pathogen of economically important food animals, is the causative agent of liver abscesses in feedlot cattle, osteomyelitis in turkeys, and pneumonia and arthritis in pigs. previous studies identified the first a. pyogenes adhesin, cbpa, a protein located on the bacterial surface which has the ability to bind collagen and promotes adhesion to the host cells. the protein has an n-terminal ligand-binding region (region a) and a c-terminal repetitive domai ...200717906137
pathology of udder lesions in sows.in recent years an increasing frequency of sows with udder lesions has been observed during meat inspection. the frequency and pattern of affected mammary complexes were determined by inspection, palpation, and incision of udders of 640 sows at a local slaughterhouse. from representative lesions, tissue specimens were sampled for histological and bacteriological examination. moreover, in seven problem cases immunohistochemistry was applied for the identification of staphylococcus aureus and arca ...200717931222
susceptibility of bacterial pathogens against lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) as determined in the bft-germvet monitoring program 2004-2006.a total of 368 bacterial pathogens, including 72 coagulase-positive and coagulase-variable staphylococci, 97 beta-haemolytic streptococci, 51 escherichia coli, 75 pasteurella multocida, 25 mannheimia haemolytica, 25 pseudomonas aeruginosa, and 23 arcanobacterium pyogenes, were investigated for their susceptibility to the three combinations of antimicrobial agents lincomycin/spectinomycin (1/2), penicillin g/neomycin (1/1), and penicillin g/dihydrostreptomycin (1/1) in comparison to their suscept ...200717939451
antimicrobial susceptibility of pseudomonas aeruginosa from dogs and cats as well as arcanobacterium pyogenes from cattle and swine as determined in the bft-germvet monitoring program 2004-2006.during the bft-germvet monitoring program, pseudomonas (p) aeruginosa from dogs and cats (n = 99) as well as arcanobacterium (a.) pyogenes from cattle and swine (n = 90) were examined for their antimicrobial susceptibility. in general, p. aeruginosa is known to be resistant against many antimicrobial agents whereas a. pyogenes is thought to be susceptible to most agents in-vitro. however, representative and actual minimum inhibitory concentration (mic) values are missing for both veterinary path ...200717939456
corynebacterium pyogenes in bull semen. 194818108844
influence of experimental intrauterine infusion of arcanobacterium pyogenes solution on ovarian activity in cycling cows.to examine the ovarian response to arcanobacterium pyogenes (a. pyogenes) in uterus, bacterial solution was infused into the uteri of cows, and the follicle and corpus luteum (cl) development were monitored with a real-time ultrasound instrument. in addition, the plasma concentrations of progesterone (p(4)) and 13, 14-dihydro-15-keto-pgf(2alpha) (pgfm) were determined. a 10 ml bacterial solution that contained a. pyogenes (8 to 15 x 10(8) cfu/ml) was infused into the uterus of eight cows transce ...200818250576
evaluation of pyelonephritis in culled indoor and outdoor high parity sows.the present trial was conducted in hungary in neighboring large indoor and outdoor pig production units, belonging to the same breeding company. rejected kidneys from 201 (out of 241; 83.4%) outdoor, and 191 (out of 512, 37.3%) indoor high parity sows, with previous history of recidiving postparturient fever and excessive postparturient vulvovaginal discharge were gross pathologically bacteriologically, and histologically evaluated. all rejected kidneys revealed chronic pyelonephritis. in outdoo ...200818265757
uterine diseases in cattle after parturition.bacterial contamination of the uterine lumen is common in cattle after parturition, often leading to infection and uterine disease. clinical disease can be diagnosed and scored by examination of the vaginal mucus, which reflects the presence of pathogenic bacteria such as escherichia coli and arcanobacterium pyogenes. viruses may also cause uterine disease and bovine herpesvirus 4 (bohv-4) is tropic for endometrial cells, causing a rapid cytopathic effect. the elimination of pathogens by the inn ...200818329302
transcriptional regulation of pyolysin production in the animal pathogen, arcanobacterium pyogenes.arcanobacterium pyogenes is an opportunistic pathogen of a number of important livestock species, and usually infects from an endogenous, commensal source. thus, as with other normal flora opportunistic pathogens, the regulation of a. pyogenes virulence factors is likely important during both commensal and pathogenic interactions with the host. the aim of this study was to investigate the regulation of a key a. pyogenes virulence factor, the cholesterol dependent cytolysin, pyolysin (plo), under ...200818514442
a simple method for the production of bacterial controls for immunohistochemistry and fluorescent in situ hybridization.immunohistochemical and fluorescent in situ hybridization (fish) assays are useful diagnostic methods for the identification of bacteria on formalin fixed paraffin embedded histological sections. to validate an anti-bacterial antibody or an oligonucleotide probe and to ensure fidelity during subsequent analyses, suitable positive and negative controls are necessary. suspensions of fixed bacteria are often used, but ideally, these controls should be fixed, embedded and processed in the same way o ...200818516690
prevalence of potentially pathogenic bacteria as genital pathogens in dairy cattle.bacteria on the genital mucosa have been studied less in healthy, non-puerperal cows than in cows with puerperal endometritis. we have thus analysed bacteria in swabs from the vagina and cervix of post-puerperal cattle (n = 644). out of the animals, 6.8% had aborted within the last 12 months, 2.6% and 11.6% showed signs of vaginitis and endometritis, respectively. in 17.2% of cervical swabs pathogenic gram-positive and in 11.5% pathogenic gram-negative bacteria were found. arcanobacterium pyogen ...200918537907
genomic characterization of arcanobacterium pyogenes isolates recovered from the uterus of dairy cows with normal puerperium or clinical metritis.arcanobacterium pyogenes is considered to be the most relevant bacterium involved in the establishment of puerperal uterine infection in cattle due to its persistence in utero, resistance to treatment and synergic action with gram negative anaerobes. once the infection is established, a. pyogenes is responsible for the persistence of the infection. the objective of this study was to characterize a. pyogenes field isolates recovered from the uterus of cows with either normal puerperium or clinica ...200818547748
fusobacterium necrophorum: a ruminal bacterium that invades liver to cause abscesses in cattle.fusobacterium necrophorum, a gram-negative, rod-shaped, and an aerotolerant anaerobe, is a normal inhabitant of the rumen of cattle. the organism is in ruminal contents and adherent to the ruminal wall. its role in ruminal fermentation is to metabolize lactic acid and degrade feed and epithelial proteins. the ruminal concentration is higher in grain-fed than forage-fed cattle. from the rumen, the organism gains entry into the portal circulation and is trapped in the liver to cause abscesses. the ...200918595747
influence of systemic fluoroquinolone administration on the presence of pasteurella multocida in the upper respiratory tract of clinically healthy calves.the influence of enrofloxacin administration (5 mg/kg) for five consecutive days on the occurrence of pasteurella multocida in the upper respiratory tract of two healthy calves was monitored over a 10-day period. from nasal swabs of two additional healthy control calves, which received a placebo saline administration, p. multocida was isolated throughout the study period. in the enrofloxacin treated calves, p. multocida was not demonstrated in the nasopharynx from 48 h after the first injection ...200818808692
safety and efficacy testing of a novel multivalent bovine bacterial respiratory vaccine composed of five bacterins and two immunogens.bovine bacterial respiratory diseases have been one of the most serious problems due to their high mortality and economic loss in calves. the vaccinations of bovine bacterial respiratory vaccines have been complex because of no multivalent vaccine. in this study, novel multivalent bovine bacterial respiratory vaccine (brv) was developed and tested for its safety and efficacy. brv was composed of two immunogens and five bacterins. these were leukotoxoid and bacterin of mannheimia haemolytica type ...200818840971
characterization of tet(32) genes from the oral metagenome.tet(32) was identified in three bacterial isolates and in metagenomic dna from the human oral cavity. the regions immediately flanking the gene were found to have similarities to the mobile elements tnb1230 from butyrivibrio fibrisolvens, ate-3 from arcanobacterium pyogenes, and ctn5 from clostridium difficile.200918955517
systemic dexamethasone and its effect on normal aerobic bacterial flora of cow.this study was carried out on 17 holestein, heifers, aged between 1 to 2 years for determining the normal aerobic bacterial flora and their changes after dexamethasone injection. swab samples were taken from eye, ear, pharynx and vagina before and 5 days after twice dexamethasone treatment. results indicated that bacillus cereus and corynebacterium pseudotuberculosis had higher frequency of isolations than the other bacterial flora in eye, ear and pharynx. actinomyces pyogenes was isolated with ...200719093453
the roles of pgf(2alpha) and pge(2) in regression of the corpus luteum after intrauterine infusion of arcanobacterium pyogenes in cows.to study the effect of bacteria in the uterus on the fate of the corpus luteum (cl), arcanobacterium pyogenes was inoculated into the uteri of cows on day 3 (day 0=day of spontaneous ovulation). plasma concentrations of 13,14-dihydro-15-keto-pgf(2alpha) (pgfm), 13,14-dihydro-15-keto-pge(2) (pgem) and progesterone (p(4)) were determined. in five cows, the developing cl regressed and first-wave dominant follicles, which normally become atretic, ovulated (group ov) after bacterial inoculation. in a ...200919095297
microbial aetiology of acute clinical mastitis and agent-specific risk factors.a nation wide study on the microbial aetiology of cases of acute clinical mastitis in swedish dairy cows was conducted with the aim to investigate changes in the microbial panorama compared to a previous study performed 1994-1995. another aim was to investigate some agent-specific environmental and individual risk factors. milk samples were collected from 987 udder quarter cases from 829 cows during six 2-month periods from may 2002 to april 2003, and data on risk factors and demography were col ...200919155148
intracranial abscessation as a natural mortality factor for adult male white-tailed deer (odocoileus virginianus) in kent county, maryland, usa.intracranial abscessation is a cause of natural mortality among free-ranging white-tailed deer (odocoileus virginianus) across portions of the united states and canada. intracranial abscesses caused by arcanobacterium pyogenes disproportionately affect adult male white-tailed deer. from 2003-08, we evaluated the occurrence of intracranial abscessation among adult (> or = 2.5 yr) radiocollared male white-tailed deer (n=33) at a large private property in kent county, maryland, usa. we documented m ...200919204349
[endocarditis due to arcanobacterium pyogenes: the first case in europe]. 200919249133
effect of intrauterine infusion of ceftiofur on uterine health and fertility in dairy cows.objectives were to determine the effects of intrauterine (i.u.) infusion of ceftiofur hydrochloride on uterine health and fertility of dairy cows already receiving pgf(2alpha) for estrous synchronization. holstein cows at 44 +/- 3 d in milk (dim) were blocked by parity and diagnosis of metritis in the first 14 dim and of other illnesses and, within each block, randomly assigned to 1 of 2 treatments: a single i.u. infusion of 125 mg of ceftiofur hydrochloride at 44 +/- 3 dim (ceftiofur, n = 396), ...200919307634
defining postpartum uterine disease and the mechanisms of infection and immunity in the female reproductive tract in cattle.uterine microbial disease affects half of all dairy cattle after parturition, causing infertility by disrupting uterine and ovarian function. infection with escherichia coli, arcanobacterium pyogenes, and bovine herpesvirus 4 causes endometrial tissue damage. toll-like receptors on endometrial cells detect pathogen-associated molecules such as bacterial dna, lipids, and lipopolysaccharide (lps), leading to secretion of cytokines, chemokines, and antimicrobial peptides. chemokines attract neutrop ...200919439727
real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing.in more than 30% of milk samples from clinical and subclinical bovine mastitis, bacteria fail to grow even after 48 h of conventional culture. the "no-growth" samples are problematic for mastitis laboratories, veterinarians, and dairy producers. this study provides the first investigation of the bacteriological etiology of such samples, using a real-time pcr-based commercial reagent kit. the assay targets the dna of the 11 most common bacterial species or groups in mastitis and the staphylococca ...200919447993
lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments.this study charted 237 fatal cases of bovine respiratory disease (brd) observed from may 2002 to may 2003 in a single oklahoma feed yard. postmortem lung samples were used for agent identification and histopathology. late in the study, 94 skin samples (ear notches) were tested for bovine viral diarrhea virus (bvdv) by immunohistochemistry (ihc). bovine respiratory disease morbidity was 14.7%, and the mortality rate of all causes was 1.3%, with more than half (53.8%) attributed to brd (0.7% total ...200919564494
identification, susceptibility, and detection of integron-gene cassettes of arcanobacterium pyogenes in bovine endometritis.the present study aimed to identify, determine the susceptibility, and detect gene cassettes of arcanobacterium (actinomyces) pyogenes isolates from cows with endometritis. arcanobacterium pyogenes isolates were identified first by using the api coryne vit system test, and further through pcr. minimum inhibitory concentrations of 23 antimicrobial agents against a. pyogenes were tested using standard broth microdilution assays according to the protocols of the clinical and laboratory standards in ...200919620647
arcanobacterium pyogenes sepsis in farmer, brazil. 200919624940
mechanisms of infertility associated with clinical and subclinical endometritis in high producing dairy cattle.clinical and subclinical endometritis are common causes of infertility and subfertility in high producing dairy cattle, delaying the onset of ovarian cyclic activity after parturition, extending luteal phases and reducing conception rates. escherichia coli and arcanobacterium pyogenes cause endometrial damage and inflammation. components of microbes, such as lipopolysaccharide (lps), are detected by toll-like receptors on endometrial cells, leading to secretion of cytokines, chemokines and antim ...200919660075
cervid herpesvirus 2, the primary agent in an outbreak of infectious keratoconjunctivitis in semidomesticated reindeer.an outbreak of infectious keratoconjunctivitis (ikc) occurred in semidomesticated reindeer (rangifer tarandus tarandus) in troms county, norway, in february 2009. twenty-eight animals with clinical symptoms and 12 apparently healthy animals were investigated. they ranged in age from calves of the year to 4-year-old animals (mean, 1.9 years; standard deviation, +/-0.9). the seroprevalence of antibodies against cervid herpesvirus 2 (cvhv2) was 86% in animals with ikc and 42% in unaffected animals. ...200919726598
studies in bovine mastitis : iii. injection of the udder with micrococci and other microorganisms.aside from the streptococci, micrococci have been the next most frequent group of organisms isolated from inflamed udders. they produce various types of disease. some give rise to only a mild catarrh of the larger milk ducts and cystern, while others produce more or less severe parenchymatous inflammation. on the whole, the prognosis is more favorable with micrococcic infection than with that associated with streptococci. cases of considerable severity have, however, been attributed to staphyloc ...191819868290
the bacteriology of bovine abortion, with special reference to acquired immunity.in a large herd of dairy cattle and young stock the bacteriological examination of 109 cases of abortion which included a relatively thorough study of the fetus and a study of the membranes, or swabs from the uterus whenever obtainable, gave the following results. 62, or 57 per cent, were associated with bacillus abortus. 26, or 23.8 per cent, were associated with spirilla. 2, or 1.8 per cent, were associated with bacillus pyogenes. 19, or 17.4 per cent, were either sterile or else the digestive ...191919868362
a study of bacillus pyogenes.bacillus pyogenes is probably quite common in this country, as it is known to be in europe. a careful study of twelve strains from cattle and one from a hog has disclosed the following characteristics which have not been reported or have been in dispute. bacillus pyogenes is gram-positive and pleomorphic, producing forms ranging from short chains of streptococcoid elements to branching filaments. it is hemolytic, producing the beta type of hemolysis in blood agar. it is not hemoglobinophilic, th ...192019868442
the etiological relation of bacillus actinoides to bronchopneumonia in calves.a bronchopneumonia of calves in the early months of life is described and its etiology associated definitely with a minute bacillus, bacillus actinoides. bacillus pyogenes, bacillus bovisepticus, and, less frequently, staphylococci and streptococci may appear later in the affected lungs. subcutaneous injections of cultures of bacillus actinoides produce large indurations ending in necrosis. similarly intratracheal injections produce circumscribed necroses of lung tissue. the cultivation of bacil ...192119868509
genomic and phenotypic characterization of escherichia coli isolates recovered from the uterus of puerperal dairy cows.the role of escherichia coli in the pathogenesis of the puerperal uterine infection of the cow is largely unknown. it is proposed that e. coli favors the persistence of arcanobacterium pyogenes and gram-negative bacteria that are pivotal to the establishment of the infection. here, we report the genomic and phenotypic characteristics of 72 e. coli isolates recovered from the uterus of dairy cows with normal puerperium (n = 12; 35 isolates) or clinical metritis (n = 18; 37 isolates), in an attemp ...200919923603
update on bacterial pathogenesis in brd.mannheimia haemolytica, pasteurella multocida, histophilus somni, mycoplasma bovis and arcanobacterium pyogenes are all frequently implicated in bovine respiratory disease (brd). m. haemolytica is considered the most important of the group. these bacteria are commensals in the nasopharynx and establish infection in the lungs of cattle that are subjected to a variety of stresses. factors that permit adherence to and proliferation in the lungs and factors that cause tissue destruction and inflamma ...200920003651
sensitivity to antibiotics of arcanobacterium pyogenes and escherichia coli from the uteri of cows with metritis/endometritis.the aim of the study was to determine the in vitro sensitivity of 161 arcanobacterium pyogenes strains and 99 escherichia coli strains isolated from the uteri of 312 cows with metritis/endometritis. animals with pathological discharges were clinically diagnosed per rectum and per vaginum, and then swabs from the lumen of the uterus were aseptically collected. bacteriological examination of swabs was according to commonly accepted procedures with the additional use of api tests. sensitivity to an ...201120129803
studies on c. pyogenes antitoxin in relation to immunity; the production of c. pyogenes antitoxin in the serum of normal dairy cows following the inoculation of alum-precipitated toxoid and of a commercial toxoid. 194720273227
studies on c. pyogenes antitoxin in relation to immunity; the effect of alum-precipitated toxoid and vaccine on the artificial infection of the udder of the ewe with c. pyogenes. 194720273228
infection with corynebacterium pyogenes in man. 194720288649
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