| enhanced toxicity for mice of combinations of bacterial lipopolysaccharide and vincristine. | sublethal doses of vincristine (vnc) and bacterial lipopolysaccharide (lps) administered simultaneously to adult male mice resulted in markedly enhanced mortality. all of 10 strains of pseudomonas aeruginosa tested, 4 of 7 strains of bacteroides, and 6 of 10 strains of listeria monocytogenes were able to substitute for purified lps in enhancing mortality in vnc-treated mice. inoculation of mice with each of 10 strains of pseudomonas, each of 7 strains of bacteroides, and about half of the 10 str ... | 1976 | 949180 |
| morphology and time course of experimental listeriosis in nude mice. | experimental listeriosis in phenotypically normal (nu/+) euthymic nmri mice has a characteristic morphology and short-term course. in contrast, listeric infection in congenitally dysthymic nude (nu/nu) mice does not proceed in clear-cut phases, develops more slowly, displays a chronic tendency from the beginning, and shows a considerably different morphology. the inability of nude mice to effectively control and terminate infection by listeria monocytogenes obviously results from the lack of t ... | 1976 | 1085754 |
| treatment options for the pharmacological therapy of neonatal meningitis. | neonatal bacterial meningitis has a relatively low incidence in developed countries, but continues to cause morbidity and mortality despite advances in antimicrobial therapy. bacterial pathogens commonly associated with neonatal meningitis include group b streptococci, escherichia coli k1 and other coliforms, listeria monocytogenes and staphylococci. as it can be difficult to differentiate meningitis from septicaemia in neonates, empirical antibiotic therapy should be effective for both. selecti ... | 1992 | 1379148 |
| a simplified, single tube, single buffer system for rna-pcr. | | 1992 | 1381195 |
| studies on the ribosomal rna operons of listeria monocytogenes. | a 23s rrna gene of listeria monocytogenes was cloned into puc19 on a 6.2-kb pst i fragment. hybridisation studies demonstrated the presence of the 5s and partial 16s rrna genes within the clone. the nucleotide sequence of the region encoding the 23s rrna was found to be highly homologous with those of other low g + c gram-positive bacteria. the 16s-23s intergenic spacer region was amplified using pcr technology and revealed two product sizes, the larger of which contained trna(ala) and trna(ileu ... | 1992 | 1383082 |
| characteristics of sodium benzoate injury of listeria monocytogenes. | over 99% of viable cells of listeria monocytogenes were injured after exposure to a solution of 8.5% sodium benzoate (ph 7.0) for 1 h. injury was evident by the inability of the bacterium to tolerate 6% nacl in tryptose agar (ta) and the ability to grow on ta with no added salt. the colony-forming ability of the injured cells was restored when they recovered in tryptose broth containing sublethal amounts of metabolic or synthetic inhibitors. synthesis of mrna was critical for restoration of salt ... | 1992 | 1383674 |
| physical map of the listeria monocytogenes chromosome. | the circular physical map of the pathogenic bacterium listeria monocytogenes lo28 (serovar 1/2c) was established by using pulsed-field gel electrophoresis. the l. monocytogenes chromosome contains eight noti fragments of 1,100, 940, 400, 335, 280, 45, 30, and 20 kb in size and eight sse8387i fragments of 860, 680, 680, 370, 335, 130, 70, and 25 kb. therefore, the total length of the genome is 3,150 kb. to order the noti fragments on the chromosome, we used a strategy which can be of general use. ... | 1992 | 1385384 |
| [purpura during listeria monocytogenes meningoencephalitis]. | | 1992 | 1387956 |
| [lymph node listeriosis in a hiv infected patient]. | | 1992 | 1387959 |
| subversion of host defense mechanisms by murine tumors. i. a circulating factor that suppresses macrophage-mediated resistance to infection. | the subcutaneous injection of cells of any one of five unselected murine tumors resulted very rapidly in the liberation into the circulation of a small molecular weight factor that severely impaired the capacity of the host to resist experimental infection with listeria monocytogenes and yersinia enterocolitica. it was found that the factor appeared in blood within 8 h of injecting tumor cells subcutaneously. that it possessed potent physiological activity was evidenced by the demonstration that ... | 1976 | 1249520 |
| spontaneous bacterial peritonitis caused by listeria monocytogenes: two case reports and literature review. | two new cases of spontaneous bacterial peritonitis (sbp) caused by listeria monocytogenes are reported. listeria monocytogenes was recovered from the ascitic fluid but not from the blood cultures of two adult diabetic inpatients with hepatic cirrhosis and sbp that had been treated empirically with cefotaxime. these two cases add to the 17 cases of listeria monocytogenes sbp reported previously, stressing the relevance of this microorganism to this clinical condition. the recovery of listeria mon ... | 1992 | 1396755 |
| [thirty years of cellular immunity]. | mackaness' original article about cellular resistance to infection, published in 1962, is recalled. these experiments laid the foundation of the analysis of immune mechanisms against infection with facultatively intracellular microorganisms. based on a simple murine model with listeria monocytogenes, an array of data was compiled during three decades, clarifying the mechanism of inborn resistance and acquired immunity to this type of parasites. the idea of various immune cell populations coopera ... | 1992 | 1398732 |
| purification and characterization of listeria monocytogenes phosphatidylinositol-specific phospholipase c. | we have purified to homogeneity the 33-kda phosphatidylinositol-specific phospholipase c (pi-plc) from the culture fluid of listeria monocytogenes, a facultative intracellular pathogen. the protein was overexpressed, and secretion of pi-plc was further enhanced by the addition of divalent cations to the culture medium. the basic protein (pi, approximately 9.4) was complexed with anionic proteins in the crude culture fluid. it bound to deae-sepharose and was eluted from sephacryl s-200 near the v ... | 1992 | 1398918 |
| early expression of cytokine mrna in mice infected with listeria monocytogenes. | protective immunity first becomes evident at 3 to 4 days after inoculation of mice with a sublethal dose of listeria monocytogenes. recent evidence suggests that production of gamma interferon (ifn-gamma) occurs earlier (within the first 24 h of infection). the purpose of this study was to define better the sequence of cytokine mrna expression during the early stages of l. monocytogenes infection. cytokine mrna expression was detected by polymerase chain reaction-assisted amplification of rna ex ... | 1992 | 1398919 |
| unipolar reorganization of f-actin layer at bacterial division and bundling of actin filaments by plastin correlate with movement of shigella flexneri within hela cells. | shigella flexneri causes bacillary dysentery, an invasive disease of colonic epithelial cells in humans. the capacity of bacteria, once they have entered into a cell and escaped the phagocytic vacuole, to spread intracellularly and directly to adjacent cells without further extracellular passage is a key factor in invasion of the epithelial layer. movement of intracellular bacteria is dependent upon the polymerization of actin; concentration of the formed filaments to one end of the bacterium is ... | 1992 | 1398922 |
| leishmania donovani infection in scid mice: lack of tissue response and in vivo macrophage activation correlates with failure to trigger natural killer cell-derived gamma interferon production in vitro. | infection of immunocompetent mice with leishmania donovani is characterized by the development of a tissue granulomatous response, in vivo macrophage activation, and a predominantly th1-type cd4+ t-cell response. to determine whether a recently described t-cell-independent pathway of gamma interferon (ifn-gamma) production involving the collaboration of macrophages and natural killer (nk) cells contributed to this pattern of events, we have investigated the responses of scid mice to l. donovani ... | 1992 | 1398944 |
| recombinant interleukin-6 protects mice against experimental bacterial infection. | because of reports of high levels of interleukin-6 (il-6) in patients during infection, we studied the role of il-6 in experimental infection. mice infected with the facultative intracellular pathogen listeria monocytogenes displayed high levels of il-6 in their sera and tissues, particularly the spleen, 1 to 3 days after infection. at this time, the il-6 titers correlated with bacterial numbers in individual mice and in groups of mice given graded doses of listeria organisms. however, the prese ... | 1992 | 1398949 |
| direct application to dairy foods of a listeria-specific oligonucleotide probe to 16s rrna. | a potentially listeria-specific 28 base oligonucleotide probe was designed from 16s rrna sequence data. using either 32p or non-radioactive (alkaline phosphatase) labels, the probe was shown to be highly specific as it hybridised to rna extracted from all of the species of listeria but not to any of the other bacteria tested. both probe methods were highly sensitive and ca 10(2) cfu/ml listeria could be detected in pure cultures. a rapid procedure for extracting rna from milk, camembert and cott ... | 1992 | 1280986 |
| in-vitro activity of rp 59500, a new semisynthetic streptogramin antibiotic, against gram-positive bacteria. | a study of the in-vitro activity of rp 59500, a semisynthetic derivative of pristinamycin, against a range of gram-positive bacteria including erythromycin-resistant strains was undertaken. mics were determined by plate dilution in isosensitest agar and mbcs by velvet pad replication. rp 59500 was found to have in-vitro activity almost identical to that of its parent compound, pristinamycin. sixty methicillin-resistant staphylococcus aureus (mrsa) and 60 methicillin-sensitive s. aureus (mssa) we ... | 1992 | 1399948 |
| a collaborative study of the in-vitro sensitivity to rp 59500 of bacteria isolated in seven hospitals in france. | the in-vitro activity of rp 59500 was determined against 1051 recent clinical bacterial isolates. the susceptibility to rp 59500 was determined with an agar dilution technique for all the isolates, while mics and mbcs were determined for 82 selected strains in broth. isolates of both staphylococcus aureus and coagulase-negative staphylococci appeared to be potentially susceptible to rp 59500, independent of susceptibility to methicillin or mls resistance. (s. aureus: methicillin-sensitive, mic90 ... | 1992 | 1399951 |
| role of lymphocytes in cellular resistance in infection. | | 1976 | 822980 |
| purification of listeriolysin o and development of an immunoassay for diagnosis of listeric infections in sheep. | a protein of 58,000-da molecular mass was purified from the supernatant fluid of a dialysis sac culture of listeria monocytogenes by cation-exchange chromatography. the purified protein, homogeneous by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and possessing the characteristics of listeriolysin o (llo), was used to develop an indirect enzyme-linked immunosorbent assay. anti-llo antibodies were shown to be consistently produced in sheep after experimental challenge with l. monocyt ... | 1992 | 1400971 |
| resistance to tumor growth mediated by listeria monocytogenes: collaborative and suppressive macrophage-lymphocyte interactions in vitro. | spleen (sc) and peritoneal cells (pc) from c57bl/6 mice immune to listeria monocytogenes (lm) have a marked cytotoxic effect on the in vitro growth of b-16 melanoma. cytotoxicity to b-16 is mediated by the interacting semsitized thymus-derived (t) lymphocytes and macrophages (mchi) or adherent cells. separation of immune sc or pc into their adherent and nonadherent components, depletion of the adnerent cells or elimination of the theata-bearing cells from an enriched t cell population all result ... | 1976 | 825574 |
| critical review of previously reported animal studies of tumor immunotherapy with nonspecific immunostimulants. | | 1976 | 826208 |
| acquired cellular resistance following transfer of lymphocytes from mice infected repeatedly with staphylococcus aureus. | | 1976 | 827335 |
| [the rapid and sensitive detection of listeria monocytogenes (the causative agent of listeriosis) by dna amplification]. | | 1992 | 1404932 |
| evaluation of a chemiluminescent dna probe assay for the rapid confirmation of listeria monocytogenes. | a listeria monocytogenes-specific, acridinium-ester-labelled dna probe was evaluated in a chemiluminescent homogeneous protection assay (hpa) for the rapid confirmation of suspect l. monocytogenes colonies from blood agar plates. the hpa uses an acridinium-ester-labelled chemiluminescent dna probe in a free-solution hybridization format. after the dna probe hybridized with the target ribosomal rna, the acridinium label on the unhybridized probe was inactivated by a chemical differential hydrolys ... | 1992 | 1410793 |
| pyrolysis mass spectrometry of listeria monocytogenes isolates from sheep. | forty-eight isolates of listeria monocytogenes from sheep and silage, involved in five small outbreaks of listeriosis, were compared by pyrolysis mass spectrometry (pyms). the method clustered isolates from single animals, and showed that epidemiologically associated isolates were closely related to each other. pyms is a simple technique capable of analysing large numbers of samples daily, and its application in veterinary studies should help to elucidate the epidemiology of listeriosis. | 1992 | 1410821 |
| contributions to further sero-variants of listeria monocytogenes. | the authors report on the isolation of 13 listeria monocytogenes strains of healthy human subjects which, within serogroup 4, contain at least four variants of known listeria antigens, but wound not fit into the grouping system that has been in use. | 1976 | 827878 |
| [the diagnosis of listeria encephalitis in ruminants using cultural and immunohistological techniques. i. comparison of different selective media and culture techniques for the detection of listeria from ruminant brains]. | the selective l-palcamy differential enrichment broth, the listeria enrichment broth of the international dairy federation, oxford listeria selective agar, and palcam listeria selective agar were comparatively examined in the cultural isolation of listeria spp. from ten ruminant brains. the l-palcamy medium proved to be superior to the idf broth in both selectivity and productivity for listeria spp. in the brain samples, which were also contaminated with other bacteria. the oxford and palcam aga ... | 1992 | 1414095 |
| t cell-monocyte interactions induced by listeria monocytogenes. | | 1992 | 1414587 |
| existing and emerging foodborne diseases. | foodborne diseases, i.e. illnesses due to contaminated food, are one of the most widespread problems of the contemporary world. they are toxic or infectious by nature and are caused by agents which enter the body through the ingestion of contaminated food or water. these agents can be chemical like pesticide residues and toxic metals or biological like pathogenic microorganisms. foods contaminated by biological agents are, however, the major cause of foodborne disease. data recorded in different ... | 1992 | 1419523 |
| listeria monocytogenes infection in an immunosuppressed patient. | | 1977 | 847965 |
| choroiditis and meningitis in experimental murine infection with listeria monocytogenes. | in a study of central nervous system involvement in experimental listeriosis 27 swiss cd1 mice were inoculated subcutaneously with listeria monocytogenes. systemic infection developed, as shown by the isolation of listeria monocytogenes and histopathological lesions in the spleen and liver. in the central nervous system a mixed inflammatory infiltration in the ventricular system, especially in the choroid plexus, and leptomeningitis were the most relevant lesions. inflammatory lesions were assoc ... | 1992 | 1425736 |
| restriction fragment length polymorphism analysis of listeria monocytogenes and its application to epidemiological investigations. | the restriction fragment length polymorphisms (rflps) of 64 random and potentially related strains of listeria monocytogenes were analysed and compared using a probe comprised of two l. monocytogenes chromosome fragments cloned into a lambda vector. twelve rflp types were defined using 14 isolates of clinical origin, 42 food isolates and eight food associated environmental strains. of the rflp types, some were common to a particular serovar and source, whereas others were widespread amongst all ... | 1992 | 1358161 |
| incidence of pathogenic bacteria in raw milk in ireland. | raw milk from 70 farms was sampled over 13 months for salmonellas, listerias, escherichia coli, staphylococcus aureus and mastitic streptococci; total bacterial counts (tbc), coliforms and somatic cells were also counted. tbc < or = 30,000/ml were obtained in 63% of samples. high count milks were found mainly during the winter months: 13% of samples had > 10(4) mastitis pathogens/ml of milk. the mean somatic cell count varied from 4.0 x 10(5) to 8.0 x 10(5)/ml throughout the year with highest co ... | 1992 | 1429309 |
| a microbiological, histopathological and immunohistological study of the intragastric inoculation of listeria monocytogenes in mice. | the course of murine infection after intragastric inoculation of l. monocytogenes was investigated by immunocytochemical, histopathological and microbiological techniques. l. monocytogenes antigen was observed in epithelial cells of intestinal mucosa overlying peyer's patches, but not in mucosa devoid of them. this suggests that penetration of l. monocytogenes into the host organism may take place through epithelium overlying peyer's patches. the efficiency of bacterial penetration appeared to b ... | 1992 | 1430342 |
| dissociation of macrophage cytolysis and ability to transfer anti-listeria resistance by concanavalin a-stimulated spleen cells. | in this study, we determined whether spleen cells from listeria monocytogenes-immunized mice were cytolytic for listeria-infected macrophages. spleen cells freshly obtained from immunized donors were unable to lyse listeria-infected macrophages unless they were first stimulated in vitro for 2-3 days with concanavalin a (cona) or l. monocytogenes. spleen cells from non-immunized mice developed cytolytic activity after incubation with cona, but not with l. monocytogenes. cytolytic spleen cells dem ... | 1992 | 1359378 |
| fetal listeriosis during the second trimester of gestation. | listeriosis is common and is implicated in about 3% of second-term abortions examined in our laboratory. maternal fever was followed rapidly in all instances by the expulsion of a nonmacerated fetus. chorioamnionitis was always present and was associated with placental microabscesses. leukocytic infiltrates were frequent in fetal tissues, being present in adrenal, lung, and skin. listeria monocytogenes was isolated from 8 of the 205 abortions that had microbiological cultures (3.9%). the clinica ... | 1992 | 1437878 |
| restriction fragment length polymorphism in four virulence-associated genes of listeria monocytogenes. | we observed restriction fragment length polymorphism in 4 genes of listeria monocytogenes associated with virulence. using the polymerase chain reaction (pcr) and primers derived from published sequences, we amplified the following genes: hlya coding for listeriolysin o, iap coding for a putative invasion-associated factor, mpl coding for a metalloprotease, and the prfa gene that positively regulates the hyla gene. pcr-amplified dna were cut with several restriction endonucleases, and the restri ... | 1992 | 1360171 |
| an unusual case of listeria meningitis. | | 1992 | 1439990 |
| effect of aflatoxin b1 on in vitro production of interleukin-1 by bovine mononuclear phagocytes. | in this study we examined the effects of preincubation with aflatoxin b1 (afb1) on the ability of bovine monocytes to produce the immunological mediator interleukin-1 (il-1). monocytes preincubated for 6-24 h with 10 micrograms ml-1 afb1 demonstrated a diminished capacity to release il-1 activity in response to bacterial lipopolysaccharide (lps). preincubation for less time, or with lower concentrations of afb1, did not affect il-1 release. pretreatment of monocytes with afb1 also resulted in di ... | 1992 | 1441224 |
| collapse of the proton motive force in listeria monocytogenes caused by a bacteriocin produced by pediococcus acidilactici. | the effect of pediocin jd, a bacteriocin produced by pediococcus acidilactici jd1-23, on the proton motive force and proton permeability of resting whole cells of listeria monocytogenes scott a was determined. control cells, treated with trypsin-inactivated bacteriocin at a ph of 5.3 to 6.1, maintained a ph gradient and a membrane potential of approximately 0.65 ph unit and 75 mv, respectively. however, these gradients were rapidly dissipated in cells after exposure to pediocin jd, even though n ... | 1992 | 1444365 |
| cd8 t cells can protect against an intracellular bacterium in an interferon gamma-independent fashion. | specific t-cell immunity to listeria monocytogenes is thought to occur through the action of lymphokines which activate phagocytes to ingest and kill microorganisms. interferon gamma (ifn-gamma) has been shown to be an effective mediator of this type of macrophage activation in vivo and in vitro. the monoclonal antibody h22.1 efficiently neutralizes endogenous ifn-gamma, exacerbates disease in a mouse model of l. monocytogenes infection, and inhibits the in vivo protective activity of a listeria ... | 1992 | 1360672 |
| genomic analysis of pediococcus starter cultures used to control listeria monocytogenes in turkey summer sausage. | the pulsed-field technique of clamped homogeneous electric field electrophoresis was employed to characterize and size genomic dna of three pediocin-producing (ped+) and two non-pediocin-producing (ped-) strains of pediococcus acidilactici. comparison of genomic fingerprints obtained by digestion with the low-frequency-cleavage endonuclease asci revealed identical restriction profiles for four of the five strains analyzed. summation of results for 10 individually sized asci fragments estimated t ... | 1992 | 1444419 |
| the antibacterial effect of some neuroleptics on strains isolated from patients with meningitis. | eighty-two strains of bacteria (neisseria meningitidis, haemophilus influenzae, enterobacteriaceae, streptococcus pneumoniae, group b streptococci and listeria monocytogenes) were examined for their in vitro susceptibility to eight drugs, seven neuroleptics (perphenazine, fluphenazine, cis(z)-clopenthixol, haloperidol, clozapine, clebopride and sch 23390), and the neuroleptically inactive trans(e)-clopenthixol. the phenothiazines and the thioxanthenes were, on the whole, the most active drugs wh ... | 1992 | 1362269 |
| effect of recombinant human granulocyte colony-stimulating factor (rh g-csf) on murine resistance against listeria monocytogenes. | recombinant human granulocyte colony-stimulating factor (rh g-csf) enhanced resistance of mice against listeria monocytogenes (lm) as determined by survival and bacterial growth. mice pretreated with rh g-csf twice daily for 5 days survived better than untreated animals to the challenge with lm. number of bacteria in peritoneal cavity (pc) and spleen was lower in treated mice than that in the control group. rh g-csf increased mainly polymorphonuclear cells (pmn) in blood and spleen. after lm ino ... | 1992 | 1374055 |
| cd8+ t cells specific for a single nonamer epitope of listeria monocytogenes are protective in vivo. | class i major histocompatibility complex (mhc)-restricted cd8+ t cells have been demonstrated to be effective mediators of both acquired and adoptive immunity to the intracellular bacterium listeria monocytogenes. we have recently determined that l. monocytogenes-infected h-2d mice recognize a nonamer peptide, residues 91-99, of the secreted protein listeriolysin o (llo), in a h-2kd-restricted fashion. in this report we have generated cd8+ t cell lines with specificity for llo 91-99 in the conte ... | 1992 | 1375265 |
| incidence of listeria monocytogenes in fresh foods in barcelona (spain). | from september 1989 to march 1990, a study of listeria spp. was carried out on 311 samples of raw foodstuffs from markets and other establishments in the city of barcelona. these foodstuffs included vegetables (103 samples), minced meats from pork, beef and poultry (168 samples) and bivalve molluscs (40 samples). l. monocytogenes was isolated in 7.8% of the vegetable samples, 17.3% of the minced meats and in 7.5% of bivalve molluscs. the most frequent serovars were 1/2 and 4. other species isola ... | 1992 | 1445758 |
| the incidence of listeria species in retail foods in japan. | meat, fish and vegetable products obtained at retail shops in or around tokyo were examined for listeria contamination. listeria spp. were isolated from 43 (56.6%) out of 76 samples of meat products. l. monocytogenes occurred in 26 (34%) of the samples, l. monocytogenes was isolated from 7 (6.1%) out of 114 samples of fish and fish products including 'ready-to-eat' foods. listeria was not isolated from any of 21 samples of vegetable and vegetable product including 'ready-to-eat' foods investigat ... | 1992 | 1445759 |
| [meningitis from listeria monocytogenes in the adult]. | we describe the characteristics of 6 adult patients with meningitis by listeria monocytogenes. five of them had previous disease and only one of these was being treated with immunosuppressors. all of them presented meningeal syndrome with cephalorhachidean fluid's pleocytosis and five of them, polymorphonuclear predominance. the empirical treatment was correct only in two cases. the mortality rate reached 50%. | 1992 | 1391576 |
| differentiation of listeria monocytogenes isolates by using plasmid profiling and multilocus enzyme electrophoresis. | three hundred and seven listeria monocytogenes isolates from various origins (clinical sources, raw chicken, seafoods, dairy and meat products and processing environments) were screened for plasmids. the overall frequency of l. monocytogenes isolates containing plasmids was 77%. the highest percentages of plasmid positive isolates were found from meat (89%), chicken (81%) and dairy products (64%), while clinical isolates had the lowest plasmid percentage (28%). seven sizes of plasmids (21, 24, 2 ... | 1992 | 1445771 |
| use of specific oligonucleotides for direct enumeration of listeria monocytogenes in food samples by colony hybridization and rapid detection by pcr. | two 18-mer oligonucleotides derived from the sequence of hly, the gene coding for listeriolysin o, were shown to be specific for listeria monocytogenes in the genus listeria in colony hybridization tests. the oligonucleotides did not hybridize with any of the bacterial species found in food and co-isolated with listeria on selective media. they were used in colony hybridization tests for enumeration of l. monocytogenes present in food samples after direct plating on selective media plates. in ad ... | 1992 | 1448613 |
| effect of nanoparticle-bound ampicillin on the survival of listeria monocytogenes in mouse peritoneal macrophages. | the efficacy of ampicillin bound to polyisohexylcyanoacrylate nanoparticles was studied in vitro in mouse peritoneal macrophages infected with listeria monocytogenes. nanoparticles containing ampicillin 1 mg/l were more effective after 30 h than free ampicillin at the same concentration, with viable counts of 3.68 and 5.43 log10 cfu/ml, respectively. the nanoparticles acted on the intracellular bacteria after a lag period of 6-9 h; this time was apparently required for the degradation of the pol ... | 1992 | 1399927 |
| typing of listeria strains by random amplification of polymorphic dna. | the polymerase chain reaction (pcr) was used to obtain randomly amplified polymorphic dna (rapd) profiles for typing of listeria strains. in this procedure, whole cells were incubated in the reaction mixture. the discriminating ability of a randomly designed 10-mer primer, hlwl74, was assessed. a total of 60 collection strains of listeria, encompassing all 7 listeria species and all known serovars was submitted to pcr with the primer hlwl74. upon agarose gel electrophoresis, 29 different banding ... | 1992 | 1448625 |
| a comparative study of randomly amplified polymorphic dna analysis and conventional phage typing for epidemiological studies of listeria monocytogenes isolates. | the analysis of rapd profiles generated by pcr with a single 10-mer, hlwl74, was compared to bacteriophage susceptibility data for epidemiological typing of listeria monocytogenes strains. a total of 104 l. monocytogenes strains was screened, all from serogroup 1 or serotype 4b. of these, 53 had been isolated during 6 different listeriosis outbreaks. the remaining 51 strains were chosen randomly from our collection. a total of 38 rapd types were observed, although each epidemic group of strains ... | 1992 | 1448626 |
| listeria monocytogenes peritonitis: case report and literature review. | listeria monocytogenes is a gram-positive bacillus that is pathogenic in both the normal and compromised host. we describe listeria peritonitis and cerebritis in a patient with cirrhosis due to non-a, non-b hepatitis, and review the 11 other cases of listeria peritonitis reported in the english-language literature. listeria is a rare cause of peritonitis in debilitated, older patients, with two-thirds of the cases occurring in patients with chronic liver disease. listeria peritonitis may also oc ... | 1992 | 1449154 |
| identification of p60 antibodies in human sera and presentation of this listerial antigen on the surface of attenuated salmonellae by the hlyb-hlyd secretion system. | antibodies directed against the major secreted protein of listeria monocytogenes, termed p60, were found more frequently than antilisteriolysin antibodies in sera of listeriosis patients. anti-p60 antibodies were also identified in all tested sera from healthy individuals. to test whether p60 provides protection against l. monocytogenes, we constructed an attenuated salmonella typhimurium aroa strain which secretes p60 via the escherichia coli hemolysin secretion pathway. application of this sal ... | 1992 | 1452342 |
| class ii mhc molecules are present in macrophage lysosomes and phagolysosomes that function in the phagocytic processing of listeria monocytogenes for presentation to t cells. | phagocytic processing of heat-killed listeria monocytogenes by peritoneal macrophages resulted in degradation of these bacteria in phagolysosomal compartments and processing of bacterial antigens for presentation to t cells by class ii mhc molecules. within 20 min of uptake by macrophages, listeria peptide antigens were expressed on surface class ii mhc molecules, capable of stimulating listeria-specific t cells. within this period, degradation of labeled bacteria to acid-soluble low molecular w ... | 1992 | 1400590 |
| induction of protective cd8+ t lymphocytes by an attenuated listeria monocytogenes acta mutant. | we tested the ability of an attenuated acta mutant of listeria monocytogenes to induce protective immunity in mice. this mutant can enter and multiply in the cytosol of the infected host cell, but is deficient in actin-dependent cell-to-cell spread. it was found to be of attenuated virulence for inbred c3h mice: the ld50 after i.v. injection was 1000-fold higher than that of the wild-type strain. mutant bacteria multiplied up to the fourth day in the liver, but only for 1 day in the spleen. a si ... | 1992 | 1286064 |
| production of an electrolyte beverage from milk permeate. | the objective of this study was to prepare shelf-stable electrolyte beverages from milk permeate. the average composition of permeate was 4.59% total solids and .40% ash. lactose was hydrolyzed (approximately 80%) with a commercial fungal lactase enzyme. additional sweetness was provided by sucrose. the ph was reduced to 3.5 to 3.8 by the addition of citric acid. shelf-stable products were made using four processes: 1) uht followed by aseptic filling, 2) heating of filled bottles to 85 degrees c ... | 1992 | 1452842 |
| listerial meningitis. which patients are vulnerable? | the physician's index of suspicion for infection with listeria monocytogenes should be elevated if a patient presents with symptoms of meningitis and has impaired cell-mediated immunity. although diagnosis is aided by detection of an elevated white blood cell count and protein level in the cerebrospinal fluid, it requires isolation of the organism from the cerebrospinal fluid. appropriate antibiotic treatment leads to recovery in most cases. | 1992 | 1454666 |
| effect of crude bacterial lipids on the course of listeria infection in mice. | crude lipids from 37 strains belonging to 32 bacterial species were isolated. by injecting mice with lipids 5 d prior to challenge with a virulent strain of listeria monocytogenes, immunostimulatory activity in 19 preparations was found. in general, lipids of gram-negative bacteria appeared to be more effective. as to bacilli, an extraordinary activity was found in the lipids of bacillus firmus. lipids of various species of the genus listeria were found to be active in approximately one-half of ... | 1992 | 1296929 |
| reduced virulence of a listeria monocytogenes phospholipase-deficient mutant obtained by transposon insertion into the zinc metalloprotease gene. | a phospholipase-deficient mutant, termed jl762, was obtained from a virulent strain of listeria monocytogenes by screening a bank of 5,000 tn1545 transposon-induced mutants on 2.5% egg yolk brain heart infusion agar. as previously shown (j. mengaud, c. geoffroy, and p. cossart, infect. immun. 59:1043-1049, 1991), the transposon insertion took place inside the gene mpl, which encodes a zinc metalloprotease. by western blot (immunoblot) analysis, we showed that loss of phospholipase activity was a ... | 1992 | 1311708 |
| a direct plating method for monitoring the contamination of listeria monocytogenes in silage. | twenty-two silage samples were analyzed for the presence of l. monocytogenes using five listeria selective plating media, with and without previous selective enrichment step. l. monocytogenes was recovered from 3 samples by both procedures, but direct plating allowed the quantification of listeria population. two of these positive samples were implicated in outbreaks of listeriosis in sheep; the l. monocytogenes population in these samples was about 10(6) cells/g. the l. monocytogenes population ... | 1992 | 1455943 |
| molecular determinants of listeria monocytogenes pathogenesis. | | 1992 | 1312514 |
| bacteriocin (monocin) interactions among listeria monocytogenes strains. | monocin interactions of 97 strains of listeria monocytogenes were assessed using an improved production method and standardisation of the monocins against the type strain of l. ivanovii. monocins were resistant to trypsin, sensitive to heating at 56 degrees c for 30 min and stable at 4 degrees c. only serovar 4 strains acted as indicators. a typing system using 8 producer and 11 indicator strains showed poor discrimination. | 1992 | 1457288 |
| minimal water activity levels for growth and survival of listeria monocytogenes and listeria innocua. | following initial range-finding experiments, total count determinations were used to determine minimal water activity (aw) levels for the growth and survival of listeria monocytogenes (l.m.) and l. innocua (l.i.). media containing three different humectants; nacl, sucrose or glycerol were used to determine minimal aw levels for growth in the above media which were 0.92, 0.92 and 0.90 respectively. the growth minima for l.i. were similar, or slightly higher than for l.m. in these media. survival ... | 1992 | 1457291 |
| a filamentous-like mutant of listeria monocytogenes with reduced expression of a 60-kilodalton extracellular protein invades and grows in 3t6 and caco-2 cells. | we describe a spontaneous rough mutant of listeria monocytogenes that produces reduced amounts of a 60-kilodalton major extracellular polypeptide (p60) as shown by sodium dodecyl sulfate--polyacrylamide gel electrophoresis and western blot analysis. the cells of this mutant are filamentous, do not give rise to smooth wild-type colonies, and produce listeriolysin o in amounts equal to that of the wild-type cells, but they show a reduced virulence in the mouse ld50 model and in the caco-2 tissue c ... | 1992 | 1458374 |
| maternal septicemia with listeria monocytogenes in second trimester without infection of the fetus. | two cases of septicemia with listeria monocytogenes in two pregnant women at the 20th and 23rd weeks of pregnancy, respectively, are presented. the women had delays of 8 and 10 days from onset of symptoms to diagnosis and adequate treatment. outcome was favorable; both women delivered healthy infants at term. | 1992 | 1322629 |
| structural and functional properties of the p60 proteins from different listeria species. | the major extracellular protein p60 of listeria monocytogenes seems to be required for this microorganism's adherence to and invasion of 3t6 mouse fibroblasts but not for adherence to human epithelial caco-2 cells. western blot analysis with polyclonal antibodies against p60 of l. monocytogenes indicated the presence of cross-reacting proteins in the culture supernatants of all listeria species. protein p60 of l. monocytogenes could restore adhesion of the l. monocytogenes mutant riii (impaired ... | 1992 | 1459966 |
| study of interactions between listeria monocytogenes and experimental tumors. | to verify the effects of listeria monocytogenes (lm) inoculation in the survival of animals bearing ehrlich's tumor. kind of study: experimental. animals-isogenic mice, balb/c, female, 19-21 g. tumor-ascitic ehrlich's tumor, dilution of 5 x 10(5) cells/0.1 ml. bacteria-lm serotype 4a, solution with 7 x 10(3) bacteria (standard sub-lethal dose). intervention-a) inoculation of lm in mice bearing ehrlich tumor at the same time as ascitic cells transplantation. b) inoculation of lm seven days before ... | 1992 | 1340994 |
| [fulminant meningoencephalitis due to listeria monocytogenes]. | we present a case of meningoencephalitis due to listeria monocytogenes type iv in a patient in which the diagnosis of small-cells malignant diffuse non-hodgkin's lymphoma had been established. he was hospitalized with vague clinical manifestations and without showing any neurological focus. hours later, he presented clinical brain stem semiology. within a 24-hour period, listeria monocytogenes was isolated from blood and cephalorhachidian fluid (being the first time that this microorganism had b ... | 1992 | 1467407 |
| destruction of bacteria on fresh meat by hot water. | strains of different bacterial genera; escherichia coli (7), salmonella serotypes (7), enteropathogenic e. coli o 157 (2), aeromonas hydrophila (4), yersinia enterocolytica (1), pseudomonas fragi (5), and listeria monocytogenes (5), inoculated onto the surface tissues of pieces of fresh beef were found to be susceptible to the lethal action of hot water. on average, more than 3 log10 (99.9%) of these organisms were destroyed when water at 80 degrees c was applied for 10 or 20 s. therefore, a hot ... | 1992 | 1468533 |
| typing of listeria monocytogenes by restriction polymorphism of the ribosomal ribonucleic acid gene region. | ninety four strains of listeria monocytogenes of different serovars and phagovars as well as of varying origins were characterized by ribosomal rna gene restriction polymorphism. after digestion by ecori or hindiii, chromosomal dnas were hybridized with a cloned rdna probe from bacillus subtilis that included the 16s rrna gene. the 94 strains were divided into 14 ribovars according to the different hybridization patterns generated by cleavage with ecori. less important genomic heterogeneity coul ... | 1992 | 1349500 |
| sequential analysis of t cells in the liver during murine listerial infection. | phenotypes and functions of t cells in the liver were studied after an i.p. inoculation with viable listeria monocytogenes in mice. t cells in the liver of untreated c3h/hen mice (c3h; h-2k, mls-2a) contain thy-1.2+tcr-alpha beta + cells as a majority and thy-1.2+tcr-gamma delta + cells and thy-1.2-tcr-gamma delta + cells as minorities. the liver of untreated c3h mice did not contain t cells expressing v beta 3 and v beta 11, which are potentially autoreactive against self-superantigens of mls-2 ... | 1992 | 1352531 |
| h-2m3 presents a listeria monocytogenes peptide to cytotoxic t lymphocytes. | we report evidence that a major histocompatibility complex-encoded nonclassic class i molecule presents a foreign peptide to cytotoxic t lymphocytes (ctl) during an infection. mice immunized with virulent listeria monocytogenes generate cd8+ ctl with alpha beta receptors specific for a bacterial peptide presented by a conserved class i molecule encoded in the m region of the major histocompatibility complex. the listeria peptide is digested by carboxypeptidase y but resists aminopeptidase m, and ... | 1992 | 1353418 |
| detection of listeria monocytogenes by non-radioactive rna probe and polymerase chain reaction. | a hindiii fragment, 651-base-pair, from the listeriolysin 0 gene of listeria monocytogenes was cloned into pgem4z. radioactive 32p labeled and non-radioactive digoxigenin labeled rna probes were made by in vitro transcriptions. these probes hybridized only to l. monocytogenes strains. polymerase chain reaction was performed by using a 22-mer and a 23-mer oligonucleotide primer, and segment of about 700-base-pair was amplified only in l. monocytogenes strains and this band was visualized by hybri ... | 1992 | 1473369 |
| kinetic analysis of cytokine gene expression in the livers of naive and immune mice infected with listeria monocytogenes. the immediate early phase in innate resistance and acquired immunity. | the anamnestic response to infection with listeria monocytogenes is characterized by the rapid elimination of normally lethal doses of bacteria and accelerated granuloma formation. these phenomena are mediated by listeria-specific memory t cells within the first 24 h after reinfection. in order to elucidate the mechanisms operative during this decisive phase of infection, we conducted a comprehensive kinetic and quantitative analysis of cytokine gene expression in the livers of naive and immune ... | 1992 | 1401926 |
| cytokine expression in vivo during murine listeriosis. infection with live, virulent bacteria is required for monokine and lymphokine messenger rna accumulation in the spleen. | to examine the regulation of cytokine synthesis during murine listeriosis, we have monitored ifn-gamma, tnf-alpha, and il-1 beta mrna levels in the spleens of c57b1/6 mice after the i.v. infusion of virulent and nonvirulent preparations of listeria monocytogenes (lm). messenger rna coding for tnf, il-1, or ifn did not become detectable until approximately 12 to 15 h after the infusion of virulent lm. levels of each cytokine mrna then increased synchronously reaching peak or near peak levels arou ... | 1992 | 1401929 |
| gemfibrozil enhances the listeriacidal effects of fluoroquinolone antibiotics in j774 macrophages. | j774 macrophage-like cells express organic anion transporters that promote the efflux of fluoroquinolone antibiotics such as norfloxacin (nfx) from these cells. gemfibrozil (gfz) blocks organic anion transport in j774 cells, thereby facilitating the intracellular accumulation of nfx (cao, c., h.c. neu, and s.c. silverstein. 1991. j. cell biol. 115:467a [abstr.]). to determine whether gfz enhances the efficacy of fluoroquinolone antibiotics against intracellular bacterial pathogens, j774 cells we ... | 1992 | 1402686 |
| role of tumor necrosis factor-alpha and interferon-gamma in newborn host defense against listeria monocytogenes infection. | the fetus and newborn are particularly susceptible to listeria monocytogenes infection. we used a newborn rat animal model to investigate neonatal host defense against listeria. in this animal model, newborn (3-d-old) rats are more susceptible to l. monocytogenes than older animals. juvenile (23-d-old) l. monocytogenes-infected rats pretreated with lipopolysaccharide (lps) had a lower bacterial load in blood than control animals, whereas lps pretreated newborn rats had a higher bacterial load. b ... | 1992 | 1437401 |
| [listeria monocytogenes endocarditis in a patient with mitral prosthesis, left auricular thrombus and adenocarcinoma of the colon]. | a case of listeria monocytogenes endocarditis in a patient with mitral prosthetic valve, left atrial thrombus and colonic adenocarcinoma is reported. vegetations were not demonstrated by transesophageal echocardiography and the clinical course was benign and without complications. cure was achieved with antibiotic therapy, and surgery was not required. these features suggest that atrial thrombus could be the source of infection. | 1992 | 1439074 |
| assessment of the accuprobe listeria monocytogenes culture identification reagent kit for rapid colony confirmation and its application in various enrichment broths. | the accuprobe listeria monocytogenes culture identification reagent kit, a nonradioactive probe, was evaluated as a colony confirmation test and in different selective or nonselective enrichment broths. the probe was 100% sensitive and 100% specific when applied to isolated colonies. the minimal detection limit in physiological saline was established to be about 10(5) cfu of l. monocytogenes. hybridization done directly in broths seeded with l. monocytogenes showed variable results. three nonsel ... | 1992 | 1476445 |
| 16s rrna-based probes and polymerase chain reaction method to detect listeria monocytogenes cells added to foods. | a rapid polymerase chain reaction (pcr) method was developed for detection of listeria monocytogenes in foods. this method used a pair of primers based on a unique region in the 16s rrna sequence of l. monocytogenes, which were previously reported by us to yield a specific nucleic acid probe. our method included use of a shorter denaturing time, a shorter annealing time, a rapid transition, and an increase in the number of cycles, resulting in good sensitivity. just 3 h for pcr plus 1 h for elec ... | 1992 | 1444393 |
| listeric septicemia with meningitis in a neonatal calf. | a 9-days-old calf which had exhibited depression and difficulty to stand and walk was examined pathologically and bacteriologically. the primary pathological changes consisted of multifocal necrosis in several visceral organs and fibrinopurulent meningitis. the necrotic lesions were most frequently found in the liver, and accompanied with mononuclear cell infiltration and gram-positive small bacilli. the organisms were also present in the foci of mononuclear cells at the central gray matter of t ... | 1992 | 1477173 |
| evaluation of the ability of primary selective enrichment to resuscitate heat-injured and freeze-injured listeria monocytogenes cells. | resuscitation rates of injured listeria monocytogenes on conventional selective listeria enrichment broth and nonselective trypticase soy broth containing 0.6% yeast extract were compared. cells were heated to 60 degrees c for 5 min or frozen at -20 degrees c for 7 days. inoculation of trypticase soy broth-yeast extract with the stressed cells resulted in growth that was superior to that in listeria enrichment broth. injured cells were fully recovered at 6 to 8 h. | 1992 | 1444432 |
| acute hepatitis by listeria monocytogenes in an hiv patient with chronic hbv hepatitis. | we report a young male ivda with cah caused by hbv who was infected with hiv and who contracted listeriosis in the form of acute hepatitis and bacteremia, with epithelioid granulomas in the liver. treatment with ampicillin and a aminoglycoside for 3 weeks was followed by rapid and maintained improvement. involvement of the liver is unusual in listeriosis and, as far as we are aware, it has not been described previously in patients with hiv infection. | 1992 | 1479174 |
| [basic and clinical study of meropenem in pediatric field]. | meropenem (mepm), a novel parenteral carbapenem antibiotic, was examined in a cooperative study involving 12 pediatric and 1 neonatologic facilities. the results are summarized as follows. 1. antibacterial activity antibacterial activity of mepm against stock organisms including 31 strains of streptococcus agalactiae, 14 of listeria monocytogenes, 4 of bordetella pertussis and 3 of neisseria meningitidis ranged from 0.025 to 0.10 micrograms/ml in mic90's, which were equal or lower than those of ... | 1992 | 1479687 |
| cloning and characterization of a gene encoding flagellin of listeria monocytogenes. | the gene, flaa, encoding the flagellin protein of listeria monocytogenes (strain 12067) has been isolated from an expression library in escherichia coli using a flagellin-specific monoclonal antibody. dna sequence analysis of a positive clone revealed the presence of an open reading frame of 287 amino acid residues with a calculated molecular mass of 30.4 kda. comparison of this sequence with flagellins from other bacteria showed a significant degree of homology in both the n- and c-terminal par ... | 1992 | 1479884 |
| discrimination of listeria monocytogenes from other listeria species by ligase chain reaction. | a ligase chain reaction assay based on a single-base-pair difference in the v9 region of the 16s rrna gene (16s rdna) was developed to distinguish between listeria monocytogenes and other listeria species. for this purpose, two pairs of primers were designed, with one primer of each pair being radioactively labeled. the ligated product was separated from the primers by denaturing polyacrylamide gel electrophoresis and then detected by autoradiography. to achieve a higher sensitivity, the 16s rdn ... | 1992 | 1482171 |
| production of bacteriocin inhibitory to listeria species by enterococcus hirae. | a bovine intestinal bacterial isolate, identified as enterococcus hirae, was found to produce a bacteriocin (designated hiraecin s) inhibitory to listeria monocytogenes and other listeria spp. identification to species level was determined by comprehensive biochemical and morphological tests which were verified by dna-dna homology assays. the antimicrobial agent was inactivated by pronase and papain and was insensitive to catalase. the antimicrobial activity was not due to hydrogen peroxide or a ... | 1992 | 1482176 |
| on-line visualization of the competitive behavior of antagonistic bacteria. | to study the interaction between cocultured listeria monocytogenes and an antagonistic leuconostoc strain producing an anti-listeria bacteriocin, flow cytometry, a technique allowing on-line and real-time analysis, was used along with classical microbiological methods. culture methods and flow cytometric measurements of the mixed culture over time point to a bactericidal action of the lactic acid-producing bacterial strain against l. monocytogenes cells. | 1992 | 1482199 |
| plasmids in listeria monocytogenes in relation to cadmium resistance. | one hundred and seventy-three unrelated listeria monocytogenes strains isolated from humans, animals, the environment, and food were analyzed for the presence of plasmids. extrachromosomal dna was found in 28% of the strains. plasmid dna was extracted more frequently from l. monocytogenes serogroup 1 strains (35%) than from serogroup 4 strains (15%). among strains from food and the environment, 40% and 29%, respectively, harbored plasmids, whereas only 13% of the strains from humans and animals ... | 1992 | 1444434 |
| [rhombencephalitis caused by listeria monocytogenes]. | the observation of an abscess in the brain stem and cerebellum due to listeria monocytogenes is presented. the patient was a 55 year old diabetic, alcoholic, gastrectomized male in whom a febrile and meningeal syndrome developed. three days later he had a progressive unilateral dysfunction of the cranial nerves. the cerebrospinal fluid showed pleocytosis of the polymorphonuclear cells, an increase in proteins and low glucose in relation with the glycemia. no parenchymatous lesion was observed up ... | 1992 | 1445713 |
| is any strain of listeria monocytogenes detected in food a health risk? | listeria spp. have been isolated from various food items. this fact does not mean in any case a true health risk. a balanced appraisal should be based on quantitative as well as qualitative aspects. actually, there is still an open debate whether a limited number of listeria has to be tolerated at least in certain food items. in addition, the pathogenic potency of an isolate may be put into account. pathogenicity of various listeria spp. definitely varies. most listeria spp., except listeria mon ... | 1992 | 1445764 |
| immunoaffinity purification, stabilization and comparative characterization of listeriolysin o from listeria monocytogenes serotypes 1/2a and 4b. | we developed a simple and highly effective procedure for stabilizing the haemolytic activity of listeriolysin o (llo) from listeria monocytogenes after immunoaffinity purification. the haemolytic activity of llo was stabilized by eluting it directly into tubes containing an alkaline buffer (5 mm lysine, 140 mm kcl, 50% ethylene glycol, ph 11.5). the purified llo retained 100% of its haemolytic activity after 6 weeks of storage at -20 degrees c. llo purified from a strain of l. monocytogenes sero ... | 1992 | 1448624 |
| the use of bacterial luciferase for monitoring the environmental regulation of expression of genes encoding virulence factors in listeria monocytogenes. | a promoter probe vector, which utilized the lux ab genes from vibrio fischeri as reporters of gene expression, was constructed for use in listeria monocytogenes. using this system gene expression can be monitored non-destructively and in real-time, simply by measuring cellular bioluminescence. derivatives of the promoter probe were constructed that contained the cloned promoters from the hlya and plca genes of l. monocytogenes. the activity of these promoters was dependent on the transcriptional ... | 1992 | 1487729 |
| purification and amino acid sequence of sakacin a, a bacteriocin from lactobacillus sake lb706. | sakacin a, a bacteriocin produced by lactobacillus sake lb706 and which inhibits the growth of listeria monocytogenes, was purified to homogeneity by ammonium sulphate precipitation and ion-exchange, hydrophobic-interaction and reversed-phase chromatography. the complete amino acid sequence of sakacin a was determined by edman degradation. the bacteriocin consisted of 41 amino acid residues and had a calculated m(r) of 4308.7, which is in good agreement with the value determined by mass spectrom ... | 1992 | 1487735 |
| characterization and purification of mesentericin y105, an anti-listeria bacteriocin from leuconostoc mesenteroides. | a leuconostoc mesenteroides ssp. mesenteroides was isolated from goat's milk on the basis of its ability to inhibit the growth of listeria monocytogenes. the antimicrobial effect was due to the presence in the culture medium of a compound, named mesentericin y105, excreted by the leuconostoc mesenteroides y105. the compound displayed known features of bacteriocins from lactic acid bacteria. it appeared as a proteinaceous molecule exhibiting a narrow inhibitory spectrum limited to genus listeria. ... | 1992 | 1487737 |