| effect of probiotics on pro-inflammatory cytokines and nf-kappab activation in ulcerative colitis. | to demonstrate the therapeutic effect of probiotics in patients with ulcerative colitis (uc), and their effect on inflammatory mediators and nuclear factor (nf)-kappab activation in these patients. | 2010 | 20806430 |
| analysis of the lactobacillus metabolic pathway. | we performed analyses of the phenotypic and genotypic relationships focusing on biosyntheses of amino acids, purine/pyrimidines, and cofactors in three lactobacillus strains. we found that lactobacillus fermentum ifo 3956 perhaps synthesized para-aminobenzoate (paba), an intermediate of folic acid biosynthesis, by an alternative pathway. | 2010 | 20817812 |
| synthesis of isoflavone aglycones and equol in soy milks fermented by food-related lactic acid bacteria and their effect on human intestinal caco-2 cells. | one hundred and three strains of lactic acid bacteria, isolated from various food ecosystems, were assayed for β-glucosidase activity toward p-nitrophenyl-β-d-glucopyranoside substrate. lactobacillus plantarum dppma24w and dppmasl33, lactobacillus fermentum dppma114, and lactobacillus rhamnosus dppmaaz1 showed the highest activities and were selected as the mixed starter to ferment various soy milk preparations, which mainly differed for chemical composition, protein dispersibility index, and si ... | 2010 | 20822177 |
| lactic acid bacteria community dynamics and metabolite production of rye sourdough fermentations share characteristics of wheat and spelt sourdough fermentations. | four spontaneous rye sourdough fermentations were performed over a period of ten days with daily back-slopping. samples taken at all refreshment steps were used for culture-dependent and culture-independent characterization of the microbiota present. furthermore, an extensive metabolite target analysis was performed through a combination of various chromatographic methods, including liquid chromatography coupled to mass spectrometry (lc/ms) and gas chromatography coupled to mass spectrometry (gc ... | 2010 | 20832677 |
| rapid evaluation of the antibiotic susceptibility of fuel ethanol contaminant biofilms. | bacterial contaminants from commercial fuel ethanol production facilities were previously shown to form biofilms as mixed cultures under laboratory conditions. in this study, a rapid assay was developed to simultaneously compare isolates for their ability to form biofilms as pure cultures. a total of 10 strains were isolated from a dry-grind fuel ethanol plant that routinely doses with virginiamycin. these were identified by sequence analysis as six strains of lactobacillus fermentum, two strain ... | 2011 | 20855199 |
| kinetic analysis of strains of lactic acid bacteria and acetic acid bacteria in cocoa pulp simulation media toward development of a starter culture for cocoa bean fermentation. | the composition of cocoa pulp simulation media (psm) was optimized with species-specific strains of lactic acid bacteria (psm-lab) and acetic acid bacteria (psm-aab). also, laboratory fermentations were carried out in psm to investigate growth and metabolite production of strains of lactobacillus plantarum and lactobacillus fermentum and of acetobacter pasteurianus isolated from ghanaian cocoa bean heap fermentations, in view of the development of a defined starter culture. in a first step, a se ... | 2010 | 20889778 |
| detection of changes in the cellular composition of salmonella enterica serovar typhimurium in the presence of antimicrobial compound(s) of lactobacillus strains using fourier transform infrared spectroscopy. | it was previously established that lactobacillus fermentum aca-dc 179, lactobacillus plantarum aca-dc 287 and lactobacillus plantarum aca-dc 2350 exhibit antimicrobial activity against salmonella enterica serovar typhimurium. in order to further investigate the killing effect of these microorganisms against salmonella cells, we employed fourier transform infrared spectroscopy (ft-ir). salmonella cells were incubated with different concentrated lactobacilli supernatants and their ft-ir spectra we ... | 2010 | 20952082 |
| immunological, antioxidative, and morphological response in combined treatment of ofloxacin and lactobacillus fermentum me-3 probiotic in salmonella typhimurium murine model. | we aimed to elucidate the immunological (cytokines), biochemical (antioxidative), and patho-morphological responses in the gut and liver evoked by the addition of lactobacillus fermentum me-3 to ofloxacin (ofx) treatment in an experimental infection model of salmonella enterica serovar typhimurium. after challenge with s. typhimurium and treatment according to different schemes, either with ofx and/or addition of l. fermentum me-3, the mice were killed. blood, liver, spleen, and small intestine ... | 2010 | 20955459 |
| intestinal bacteroides species associated with coeliac disease. | to characterise the predominant species of bacterial populations associated with duodenal biopsies of paediatric patients with active and treated coeliac disease. | 2010 | 20972239 |
| investigations of amino acids, peptides, and proteins; the determination of methionine in protein hydrolysates with lactobacillus fermenti 36. | | 1946 | 20985629 |
| investigations of amino acids, peptides, and proteins; the determination of threonine in protein hydrolysates with lactobacillus fermenti 36. | | 1946 | 20985630 |
| investigations of amino acids, peptides, and proteins; the amino acid requirements of lactobacillus fermenti 36. | | 1945 | 21006947 |
| investigations of amino acids, peptides, and proteins; the determination of histidine in protein hydrolysates with lactobacillus fermenti 36. | | 1945 | 21006948 |
| effects of ensilage on storage and enzymatic degradability of sugar beet pulp. | ensiling was investigated for the long-term storage of sugar beet pulp (sbp). eight strains of lactic acid bacteria (lab) and a non-inoculated control were screened based on their ability to rapidly reduce ph, produce a large amount of lactic acid and inhibit undesirable fermentations. lactobacillus brevis b-1836 (lab #120), lactobacillus fermentum nrrl b-4524 (lab #137) and a non-inoculated control were selected for further research to determine the effects of lab inoculation level and packing ... | 2010 | 21036038 |
| complete glutathione system in probiotic lactobacillus fermentum me-3. | there is much information about glutathione (gsh) in eukaryotic cells, but relatively little is known about gsh in prokaryotes. without gsh and glutathione redox cycle lactic acid bacteria (lab) cannot protect themselves against reactive oxygen species. previously we have shown the presence of gsh in lactobacillus fermentum me-3 (dsm14241). results of this study show that probiotic l. fermentum me-3 contains both glutathione peroxidase and glutathione reductase. we also present that l. fermentum ... | 2010 | 21058502 |
| isoflavone phytoestrogens in soymilk fermented with β-glucosidase producing probiotic lactic acid bacteria. | isoflavones significantly contribute to human health and disease prevention, and exist as glucosides and aglycones. the β-glucosidase enzyme that hydrolyzes glucosides to aglycones is very sensitive to molecular structure, and thus the profile of the isoflavones can affect their rate of hydrolysis. soymilk was fermented with lactobacillus acidophilus b4496 (la), lactobacillus bulgaricus cfr2028 (lb), lactobacillus casei b1922 (lc), lactobacillus plantarum b4495 (lp) and lactobacillus fermentum b ... | 2010 | 21091296 |
| genome analysis of lactobacillus fermentum temperate bacteriophage фpyb5. | the complete genomic sequence of lactobacillus fermentum temperate bacteriophage фpyb5 was determined. the phage possesses a linear, double-stranded dna genome of 32,847 bp with a g+c content of 45.21%. a total of 46 putative open reading frames (orfs) were identified. on the basis of homology comparisons, 25 orfs could be assigned putative functions. the genome of bacteriophage фpyb5 is highly modular with functionally related genes clustered together. genome dna of temperate bacteriophage фpyb ... | 2010 | 21111501 |
| microbiota of dentinal caries as assessed by reverse-capture checkerboard analysis. | this study aimed to identify the microbiota of different layers of dentinal caries by using a culture-independent molecular biology approach. | 2010 | 21160186 |
| lactic acid bacteria fermentation of human milk oligosaccharide components, human milk oligosaccharides and galactooligosaccharides. | human milk contains about 7% lactose and 1% human milk oligosaccharides (hmos) consisting of lactose with linked fucose, n-acetylglucosamine and sialic acid. in infant formula, galactooligosaccharides (goss) are added to replace hmos. this study investigated the ability of six strains of lactic acid bacteria (lab), lactobacillus acidophilus, lactobacillus plantarum, lactobacillus fermentum, lactobacillus reuteri, streptococcus thermophilus and leuconostoc mesenteroides subsp. cremoris, to digest ... | 2010 | 21175746 |
| virus disinfection in water by biogenic silver immobilized in polyvinylidene fluoride membranes. | the development of innovative water disinfection strategies is of utmost importance to prevent outbreaks of waterborne diseases related to poor treatment of (drinking) water. recently, the association of silver nanoparticles with the bacterial cell surface of lactobacillus fermentum (referred to as biogenic silver or bio-ag(0)) has been reported to exhibit antiviral properties. the microscale bacterial carrier matrix serves as a scaffold for ag(0) particles, preventing aggregation during encapsu ... | 2010 | 21183198 |
| predominant genera of fecal microbiota in children with atopic dermatitis are not altered by intake of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium animalis subsp. lactis bi-07. | the effect of probiotic bacteria lactobacillus acidophilus ncfm and bifidobacterium lactis bi-07 on the composition of the lactobacillus group, bifidobacterium and the total bacterial population in feces from young children with atopic dermatitis was investigated. the study included 50 children randomized to intake of one of the probiotic strain or placebo. microbial composition was characterized by denaturing gradient gel electrophoresis, quantitative pcr and, in a subset of subjects, by pyrose ... | 2011 | 21204871 |
| intraspecies discrimination of lactobacillus paraplantarum by pcr. | lactobacillus paraplantarum is a species phenotypically close to lactobacillus plantarum. several pcr methods were evaluated to discriminate l. paraplantarum strains and among them, a pcr using an enterobacterial repetitive intergenic consensus (eric) sequence differentiated l. paraplantarum from other lactobacillus species. in addition, a combination of eric and random amplified polymorphic dna (rapd) analysis distinguished among seven strains of l. paraplantarum tested. eric-pcr profiles showe ... | 2011 | 21204929 |
| characterisation of lactobacillus fermentum sm-7 isolated from koumiss, a potential probiotic bacterium with cholesterol-lowering effects. | lactic acid bacteria (lab) have been demonstrated to have cholesterol-reducing effects in many studies. | 2010 | 21218486 |
| phylogenetic group- and species-specific oligonucleotide probes for single-cell detection of lactic acid bacteria in oral biofilms. | the purpose of this study was to design and evaluate fluorescent in situ hybridization (fish) probes for the single-cell detection and enumeration of lactic acid bacteria, in particular organisms belonging to the major phylogenetic groups and species of oral lactobacilli and to abiotrophia/granulicatella. | 2011 | 21247450 |
| potential beneficial properties of bacteriocin-producing lactic acid bacteria isolated from smoked salmon. | aims: to evaluate the probiotic properties of strains isolated from smoked salmon and previously identified as bacteriocin producers. methods and results: strains lactobacillus curvatus et06, et30 and et31, lactobacillus fermentum et35, lactobacillus delbrueckii et32, pediococcus acidilactici et34 and enterococcus faecium et05, et12 and et88 survived conditions simulating the gastrointestinal tract (git) and produced bacteriocins active against several strains of listeria monocytogenes, but pr ... | 2011 | 21251174 |
| integrative expression system for delivery of antibody fragments by lactobacilli. | a series of expression cassettes which mediate secretion or surface display of antibody fragments was stably integrated in the chromosome of lactobacillus paracasei. l. paracasei producing surface-anchored variable domain of llama heavy chain (vhh) (arp1) directed against rotavirus showed efficient binding to rotavirus and protection in the mouse model of rotavirus infection. | 2011 | 21257814 |
| analysis of bacterial communities of traditional fermented west african cereal foods using culture independent methods. | in this study, the microbial composition of kunu-zaki and ogi, two popular foods in nigeria produced after natural, uncontrolled fermentation of cereals, was assessed by culture-independent molecular profiling methods. in particular, pcr-denaturing gradient gel electrophoresis and construction of 16s rrna gene clone libraries revealed the presence of diverse bacterial communities. dna sequencing of the highly variable v3 region of the 16s rrna genes obtained from pcr-dgge fingerprints identified ... | 2011 | 21272950 |
| diversity and dynamics of bacterial populations during spontaneous sorghum fermentations used to produce ting, a south african food. | ting is a spontaneously fermented sorghum food that is popular for its sour taste and unique flavour. insight of the microbial diversity and population dynamics during sorghum fermentations is an essential component of the development of starter cultures for commercial production of ting. in this study, bacterial populations associated with spontaneous sorghum fermentations were examined using a culture-independent strategy based on denaturing gradient gel electrophoresis and sequence analysis o ... | 2011 | 21300507 |
| phenotypic and genotypic analyses of lactic acid bacteria in local fermented food, breast milk and faeces of mothers and their babies. | lactic acid bacteria (lab) are generally accepted as beneficial to the host and their presence is directly influenced by ingestion of fermented food or probiotics. while the intestinal lactic microbiota is well-described knowledge on its routes of inoculation and competitiveness towards selective pressure shaping the intestinal microbiota is limited. in this study, lab were isolated from faecal samples of breast feeding mothers living in syria, from faeces of their infants, from breast milk as w ... | 2011 | 21300508 |
| probiotic factors partially improve parameters of 5-fluorouracil-induced intestinal mucositis in rats. | certain live bacteria have demonstrated preliminary indications of efficacy for the treatment of chemotherapy-induced intestinal mucositis. however, probiotic derived supernatants (sn) have yet to be investigated in the mucositis setting. we evaluated sn from escherichia coli nissle 1917 (ecn) and lactobacillus fermentum br11 (br11) for their capacity to decrease 5-fluorouracil (5-fu)-induced damage in vivo. female dark agouti rats were gavaged with 1 ml of either sn or vehicle daily (days 0-8) ... | 2011 | 21307648 |
| [genetic identification of antagonistically active lactobacillus strains isolated from the oral cavity of healthy individuals]. | examination of dental deposits from 45 healthy individuals detected 3 lactobacillus strains showing a high antagonism toward test cultures. the api 50 ch "bio merieux" test systems were employed to identify strains as lactobacillus fermentum 39, lactobacillus rhamnosus 24 and lactobacillus paracasei 50. the results of analyzing the sequences of the 16s rrna genes of the test strains confirmed this identification, except for the latter strain. the taxonomic status of the third strain l. rhamnosus ... | 2010 | 21313755 |
| an in vitro protocol for direct isolation of potential probiotic lactobacilli from raw bovine milk and traditional fermented milks. | a method for isolating potential probiotic lactobacilli directly from traditional milk-based foods was developed. the novel digestion/enrichment protocol was set up taking care to minimize the protective effect of milk proteins and fats and was validated testing three commercial fermented milks containing well-known probiotic lactobacillus strains. only probiotic bacteria claimed in the label were isolated from two out of three commercial fermented milks. the application of the new protocol to 1 ... | 2011 | 21318359 |
| influence of temperature and backslopping time on the microbiota of a type i propagated laboratory wheat sourdough fermentation. | sourdough fermentation is a cereal fermentation that is characterized by the formation of stable yeast/lactic acid bacteria (lab) associations. it is a unique process among food fermentations in that the lab that mostly dominate these fermentations are heterofermentative. in the present study, four wheat sourdough fermentations were carried out under different conditions of temperature and backslopping time to determine their effect on the composition of the microbiota of the final sourdoughs. a ... | 2011 | 21335386 |
| dynamics and species diversity of communities of lactic acid bacteria and acetic acid bacteria during spontaneous cocoa bean fermentation in vessels. | to speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was developed successfully. therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20 kg scale were tried out in triplicate. the community dynamics and kinetics of these fermentations were studied through a multiphasic approach. microbiological analysis revealed a limited bacterial s ... | 2010 | 21356451 |
| [disorganization of biofilms of clinical strains of staphylococci by metabolites of lactobacilli]. | to study morpho-physiologic characteristics of clinical biofilm-forming strains of staphylococcus aureus and s. epidermidis suppressed by metabolites of lactobacilli, which produce bacteriocin-like substances. | 2010 | 21381381 |
| proteolysis by lactobacillus fermentum ifo3956 isolated from egyptian milk products decreases immuno-reactivity of +¦s1-casein. | proteinase activity of lactobacillus fermentum ifo3956 cells was higher when they were grown on milk-based media than on 10% reconstituted skim milk. the lowest protease activity was observed when cells were grown on milk-free media. the extraction of milk-induced cell-bound proteases from lb. fermentum ifo3956 was most efficient using 1% tween 80 while the use of 1% sds inhibited all proteolytic activity. two bands of ôê+35 and >100 kda were observed by zymogram, indicating that proteolytic act ... | 2011 | 21385515 |
| (gtg)(5)-pcr fingerprinting of lactobacilli isolated from cervix of healthy women. | a group of lactobacilli isolated from the cervix of 31 healthy women was characterized by (gtg)(5)-polymerase chain reaction (pcr) fingerprinting in order to evaluate this method for identification of vaginal lactobacilli. obtained fingerprints were compared with profiles available in an in-house database of the ccm bacteria collection covering type and reference strains of multiple lactic acid bacteria including lactobacilli. selected strains representing individual clusters were further identi ... | 2011 | 21390569 |
| the antibacterial activity of biogenic silver and its mode of action. | in a previous study, biogenic silver nanoparticles were produced by lactobacillus fermentum which served as a matrix preventing aggregation. in this study the antibacterial activity of this biogenic silver was compared to ionic silver and chemically produced nanosilver. the minimal inhibitory concentration (mic) was tested on gram-positive and gram-negative bacteria and was comparable for biogenic silver and ionic silver ranging from 12.5 to 50 mg/l. in contrast, chemically produced nanosilver h ... | 2011 | 21468709 |
| lactobacillus fermentum (pcc®) supplementation and gastrointestinal and respiratory-tract illness symptoms: a randomised control trial in athletes. | abstract: | 2011 | 21477383 |
| the anti-biofouling effect of lactobacillus fermentum-derived biosurfactant against streptococcus mutans. | biofouling in the oral cavity often causes serious problems. the ability of streptococcus mutans to synthesize extracellular glucans from sucrose using glucosyltransferases (gtfs) is vital for the initiation and progression of dental caries. recently, it was demonstrated that some biological compounds, such as secondary metabolites of probiotic bacteria, have an anti-biofouling effect. in this study, s. mutans was investigated for the anti-biofouling effect of lactobacillus fermentum (l.f.)-deri ... | 2011 | 21526440 |
| lactobacillus fermentum isolated from human colonic mucosal biopsy inhibits the growth and adhesion of enteric and foodborne pathogens. | a number of lactobacillus species are used as probiotic strains in order to benefit health. we have isolated l. fermentum from human colonic mucosal biopsy samples that possess antimicrobial activities against entroinvasive and foodborne pathogens such as escherichia coli, salmonella paratyphi a, shigella sonnei, staphylococcus aureus, enterococcus faecalis, proteus mirabilis, pseudomonas aeruginosa, and vibrio sp. in addition to lactic acid, l. fermentum secretes antimicrobial proteinacious com ... | 2010 | 21535608 |
| adherent properties and macrophage activation ability of 3 strains of lactic acid bacteria. | lactic acid bacteria (lab) must possess probiotic properties in order to be beneficial to humans and animals. the adherent properties, the acid and bile tolerance as well as the macrophage activation ability of isolated lab strains were investigated in this study. the adhesion was analyzed following heat, acid, trypsin, and sodium periodate treatments. production of the cytokine tumor necrosis factor-α (tnf-α) by raw 264.7 macrophages was also measured after stimulation with heat-killed lab stra ... | 2011 | 21535686 |
| application of lactobacillus fermentum hl57 and pediococcus acidilactici sp979 as potential probiotics in the manufacture of traditional iberian dry-fermented sausages. | the purpose of this study was to investigate the potential of implanting the probiotics lactobacillus fermentum hl57 and p. acidilactici sp979 during manufacture of iberian dry-fermented sausages, and their effect on the sensory properties of these meat products. microbial counts were studied in sausages inoculated with these strains and compared with a control batch. to detect the presence of probiotic strains at high counts (>7 log cfu/g) in the inoculated sausages, specific pcr and (rapd)-pcr ... | 2011 | 21569925 |
| comparison of the bacterial species diversity of spontaneous cocoa bean fermentations carried out at selected farms in ivory coast and brazil. | to compare the spontaneous cocoa bean fermentation process carried out in different cocoa-producing regions, heap and box (one ivorian farm) and box (two brazilian farms) fermentations were carried out. all fermentations were studied through a multiphasic approach. in general, the temperature inside the fermenting mass increased throughout all fermentations and reached end-values of 42-48°c. the main end-products of pulp carbohydrate catabolism were ethanol, lactic acid, acetic acid, and/or mann ... | 2011 | 21569940 |
| the effect of lactobacillus fermentum on beta2 toxin production by clostridium perfringens. | clostridium perfringens although a member of the normal gut flora is also an important cause of intestinal disease in animals and, to a lesser extent, in humans. disease is associated with the production of one or more toxins and little is known about environmental influences on the production of these toxins. one of the health promoting effects of lactic acid bacteria (lab) is the establishment and maintenance of a low ph in the intestine since an acidic environment inhibits the growth of many ... | 2011 | 21602389 |
| prevalence and impact of single-strain starter cultures of lactic acid bacteria on metabolite formation in sourdough. | flavour of type ii sourdoughs is influenced by the ingredients, processing conditions, and starter culture composition. it is, however, not fully clear to what extent different sourdough lactic acid bacteria (lab) contribute to flavour. therefore, two types of flour (rye and wheat) and different lab starter culture strains were used to prepare sourdoughs, thereby leaving the yeast microbiota uncontrolled. all lab starter culture strains tested were shown to be prevalent and to acidify the flour/ ... | 2011 | 21645811 |
| complete genomic sequence of the lactobacillus temperate phage lf1. | bacteriophage lf1, a newly isolated temperate phage from a mitomycin-c-induced lysate of wild type lactobacillus fermentum, was found to contain a double-strand dna of 42,606 base pairs (bp) with a g+c content of 45%. bioinformatic analysis of the phage genome revealed 57 putative open reading frames (orfs). the predicted protein products of orfs were determined and described. according to morphological analysis by transmission electron microscopy (tem), lf1 has an isometric head and a non-contr ... | 2011 | 21811858 |
| antifungal activity of lactobacillus fermentum te007, pediococcus pentosaceus te010, lactobacillus pentosus g004, and l. paracasi d5 on selected foods. | in the search for new preservatives from natural resources to replace or to reduce the use of chemical preservatives 4 strains of lactic acid bacteria (lab) were selected to be evaluated for their antifungal activity on selected foods. the supernatants of the selected strains delayed the growth of fungi for 23 to 40 d at 4 °c and 5 to 6 d at 20 and 30 °c in tomato puree, 19 to 29 d at 4 °c and 6 to 12 d at 20 and 30 °c in processed cheese, and 27 to 30 d at 4 °c and 12 to 24 d at 20 and 30 °c in ... | 2011 | 21806613 |
| ureaplasma urealyticum and mycoplasma hominis sensitivity to bacteriocins produced by two lactobacilli strains. | the purpose of the present study was to determine the inhibitory activities of two bacteriocins, produced by lactobacilli, against genital mycoplasmas. in this study, infections produced by genital mycoplasmas were studied; of these, 1.3% were caused by mycoplasma hominis, 10.7% by ureaplasma urealyticum and 5.6% by u. urealyticum + m. hominis. u. urealyticum was isolated from 75 out of 123 patients with genital mycoplasmas, while m. hominis was isolated from 9 patients (7.3%) and both u. urealy ... | 2011 | 21805206 |
| simulated cocoa pulp fermentations of cocoa-specific lactic acid bacteria reveal interesting starter culture strains for controlled cocoa bean fermentation. | among various lactic acid bacteria strains tested, cocoa-specific strains of lactobacillus fermentum were best adapted to the cocoa pulp ecosystem. they fermented glucose to lactic acid and acetic acid, reduced fructose to mannitol, and converted citric acid into lactic acid and 2,3-butanediol. | 2011 | 21803901 |
| inhibitory effect of oral lactobacillus against oral pathogens. | aim: to determine the inhibitory effect of oral lactobacillus against putative oral pathogens. methods and results: total 357 strains comprising 10 species of oral lactobacillus, lactobacillus fermentum (195), lactobacillus salivarius (53), lactobacillus casei (20), lactobacillus gasseri (18), lactobacillus rhamnosus (14), lactobacillus paracasei (12), lactobacillus mucosae (12), lactobacillus oris (12), lactobacillus plantarum (11) and lactobacillus vaginalis (10) were used as producer strain ... | 2011 | 21801186 |
| [in process citation]. | | 2011 | 21744644 |
| safety and persistence of orally administered human lactobacillus sp. strains in healthy adults. | the aim of the study was to evaluate the safety and persistence of selected lactobacillus strains in the gastrointestinal tract (git) of healthy adult volunteers after oral consumption of high doses of lactobacilli to identify potential candidates for probiotic and biotechnological applications. in the first phase of the study, nine individuals consumed capsules containing lactobacillus gasseri 177 and e16b7, lactobacillus acidophilus 821-3, lactobacillus paracasei 317 and lactobacillus fermentu ... | 2011 | 21831792 |
| anti-infective properties of lactobacillus fermentum against staphylococcus aureus and pseudomonas aeruginosa. | surgical wounds and implant-associated staphylococcus aureus and pseudomonas aeruginosa infections are often difficult to treat because of limited susceptibility of several of these strains to conventional antibiotics. as a result, there is a constant need for new alternative drugs. the aim of this study was to investigate the antimicrobial properties of lactobacillus fermentum, a probiotic bacterium, which we have isolated from colonic biopsies. the inhibition of s. aureus and p. aeruginosa gro ... | 2011 | 21701187 |
| effects of two lactobacillus strains on lipid metabolism and intestinal microflora in rats fed a high-cholesterol diet. | the hypocholesterolemic effects of lactic acid bacteria (lab) have now become an area of great interest and controversy for many scientists. in this study, we evaluated the effects of lactobacillus plantarum 9-41-a and lactobacillus fermentum m1-16 on body weight, lipid metabolism and intestinal microflora of rats fed a high-cholesterol diet. | 2011 | 21722398 |
| suppression subtractive hybridization identifies bacterial genomic regions that are possibly involved in hbd-2 regulation by enterocytes. | scope: human +¦-defensin 2 (hbd-2) is an inducible antimicrobial peptide synthesized by the epithelium to counteract bacterial adherence and invasion. it has been suggested that probiotic bacteria sustain gut barrier function via induction of defensins. the goals of this study were (i) to evaluate the potential immunomodulatory effects of 11 different lactobacillus fermentum strains isolated from kimere, an african fermented pearl millet (pennisetum glaucum) dough, on the hbd-2 secretion by huma ... | 2011 | 21710560 |
| spontaneous organic cocoa bean box fermentations in brazil are characterized by a restricted species diversity of lactic acid bacteria and acetic acid bacteria. | spontaneous organic cocoa bean box fermentations were carried out on two different farms in brazil. physical parameters, microbial growth, bacterial species diversity [mainly lactic acid bacteria (lab) and acetic acid bacteria (aab)], and metabolite kinetics were monitored, and chocolates were produced from the fermented dry cocoa beans. the main end-products of the catabolism of the pulp substrates (glucose, fructose, and citric acid) by yeasts, lab, and aab were ethanol, lactic acid, mannitol, ... | 2011 | 21839382 |
| diversity of lactic acid bacteria associated with traditional fermented dairy products in mongolia. | spontaneous milk fermentation has a long history in mongolia, and beneficial microorganisms have been handed down from one generation to the next for use in fermented dairy products. the objective of this study was to investigate the diversity of lactic acid bacteria (lab) communities in fermented yak, mare, goat, and cow milk products by analyzing 189 samples collected from 13 different regions in mongolia. the lab counts in these samples varied from 3.41 to 9.03 log cfu/ml. fermented yak and m ... | 2011 | 21700007 |
| identification and characterisation of lactobacilli isolated from kimere, a spontaneously fermented pearl millet dough from mbeere, kenya (east africa). | the objective of the study was to isolate potential probiotic lactobacilli from kimere, a pearl millet dough prepared in the mbeere community of kenya, east africa, by fermentation for 18-24 hours. kimere samples, collected from 11 different homesteads in mbeere, showed average ph values of 3.63-¦0.29. counts of presumptive lactobacilli were 8.52-¦0.02 log10 colony forming units per gram, respectively. 48 presumptive lactobacillus isolates were characterised and identified by biochemical and mol ... | 2010 | 21831760 |
| Effect of grape polyphenols on lactic acid bacteria and bifidobacteria growth: Resistance and metabolism. | Food polyphenols are able to selectively modify the growth of susceptible micro-organisms. This study describes the effect of a flavan-3-ol enriched grape seed extract (GSE) on the growth of several lactic acid bacteria (LAB) and bifidobacteria and the ability of the resistant strains to metabolize these compounds. Streptococcus thermophilus, Lactobacillus fermentum, Lactobacillus acidophilus and Lactobacillus vaginalis strains showed a remarkable sensitivity to the phenolic extracts assayed, in ... | 2011 | 21839384 |
| lactobacillus fermentum cect 5716 is safe and well tolerated in infants of 1-6 months of age: a randomized controlled trial. | the objective of the study was to evaluate the safety and tolerance of an infant formula supplemented with lactobacillus fermentum cect5716, a probiotic strain isolated from breast milk, in infants of 1-6 months of age. a randomized double blinded controlled study including healthy infants was conducted. one month aged infants received a prebiotic infant formula supplemented with l. fermentum (experimental group) or the same formula without the probiotic strain (control group) for 5 months. the ... | 2011 | 22155106 |
| Characterization of tetracycline resistance lactobacilli isolated from swine intestines at western area of Taiwan. | To investigate the frequency of tetracycline resistance (Tet-R) lactobacilli in pig intestines, a total of 256 pig colons were analyzed and found to contain typical colonies of Tet-R lactic acid bacteria in every sample, ranging from 3.2 × 10(3) to 6.6 × 10(5) CFU/cm(2). From these samples, a total of 159 isolates of Tet-R lactobacilli were obtained and identified as belonging to 11 species, including Lactobacillus reuteri, Lactobacillus amylovorus, Lactobacillus salivarius, Lactobacillus planta ... | 2011 | 21906691 |
| structure-function relationships of the antibacterial activity of phenolic acids and their metabolism by lactic acid bacteria. | to determine structure-function relationships of antibacterial phenolic acids and their metabolites produced by lactic acid bacteria (lab). | 2011 | 21895894 |
| screening of lactic acid bacteria and bifidobacteria for potential probiotic use in iberian dry fermented sausages. | the purpose of this study was to select lactic acid bacteria and bifibobacteria strains as potential probiotic cultures during the processing of iberian dry fermented sausages. a total of 1000 strains were isolated from iberian dry fermented sausages (363), and human (337) and pig faeces (300) in different culture media. around 30% of these strains, mainly isolated from iberian dry fermented sausages in lamvab agar, were pre-selected for testing as potential probiotics by their ability to grow a ... | 2008 | 22063588 |
| Production of xylooligosaccharides from corncob xylan by fungal xylanase and their utilization by probiotics. | The selective production of xylooligosaccharides (XOS) was carried out using partially purified xylanase from Aspergillus foetidus MTCC 4898. Corncob xylan was extracted using a mild alkali treatment which yielded 178.73±5.8gofxylan/kg of corncobs. Partially purified ß-xylosidase free xylanase was found efficient in releasing xylooligosaccharides from corncob xylan. Maximum yield of xylooligosaccharides was 6.73±0.23mg/ml after 8h of reaction time using 20U of xylanase at 45°C. Purification of X ... | 2011 | 22100233 |
| [selection and genotyping of lactobacillus with potential preventive effect by repetitive element sequence-based pcr analysis]. | we selected and characterized isolates of lactobacillus crispatus (l. crispatus) for potential preventing infections of the female reproductive tract. we cultured vaginal swabs from healthy volunteers on de man, rogosa and sharpe (mrs) agar and identified the isolates at the species level by 16s rrna sequence and genotyped the isolates of lactobacillus by pcr amplification of repetitive bacterial dna elements (rep-pcr). furthermore, 10 l. crispatus strains were assessed for hydrogen peroxide (h2 ... | 2011 | 21845850 |
| Genetic screening of functional properties of lactic Acid bacteria in a fermented pearl millet slurry and in the metagenome of fermented starchy foods. | Lactic acid bacteria (LAB) (n = 152) in African pearl millet slurries and in the metagenomes of amylaceous fermented foods were investigated by screening 33 genes involved in probiotic and nutritional functions. All isolates belonged to six species of the genera Pediococcus and Lactobacillus, and Lactobacillus fermentum was the dominant species. We screened the isolates for the abilities to survive passage through the gastrointestinal tract and to synthesize folate and riboflavin. The isolates w ... | 2011 | 22003019 |
| cytotoxic and antimicrobial aporphine alkaloids from fissistigma poilanei (annonaceae) collected in vietnam. | two new aporphine alkaloids: 8-hydroxy-9-methoxy-1,2-methylenedioxyaporphine (1) and 8-hydroxy-3,9-dimethoxy-1,2-methylenedioxyaporphine (2) were isolated from the ethyl acetate extract of fissistigma poilanei along with five known compounds: oxocrebanine (3), kuafumine (4), (2r,3r)-3',4',5,7-tetrahydroxydihydroflavonol-3-o-α-l-rhamnopyranoside (5), (+)-catechin 3-o-α-l-rhamnopyranoside (6) and quercetine 3,7-dimethoxy-3'-o-α-l-rhamnopyranosyl-(1 → 2)-β-d-glucopyranoside (7). these two new aporp ... | 2011 | 22077457 |
| purification and characterization of fructan and fructansucrase from lactobacillus fermentum akj15 isolated from kodo ko jaanr, a fermented beverage from north-eastern himalayas. | fructansucrase and fructan produced from lactobacillus fermentum akj15 were isolated from seeds of kodo ko jaanr, a fermented mild-alcoholic beverage prepared in north east india. the strain was identified by 16s rrna gene sequence analysis and biochemical characterization. the strain displayed maximum fructansucrase activity of 4.3 u/ml (1.02 u/mg) at 28°c at 180 rpm. the enzyme purified by polyethylene glycol-400 gave specific activity of 5 u/mg and showed 90 kda band on non-denaturing sodium ... | 2011 | 21942885 |
| production of d: -tagatose, a functional sweetener, utilizing alginate immobilized lactobacillus fermentum cgmcc2921 cells. | d: -tagatose is a ketohexose that can be used as a novel functional sweetener in foods, beverages, and dietary supplements. this study was aimed at developing a high-yielding d: -tagatose production process using alginate immobilized lactobacillus fermentum cgmcc2921 cells. for the isomerization from d: -galactose into d: -tagatose, the immobilized cells showed optimum temperature and ph at 65 °c and 6.5, respectively. the alginate beads exhibited a good stability after glutaraldehyde treatment ... | 2011 | 22203394 |
| administration of lactobacillus fermentum crl1446 increases intestinal feruloyl esterase activity in mice. | to evaluate the effect of oral administration of lactobacillus fermentum crl1446 on the intestinal feruloyl esterase (fe) activity and oxidative status of mice. | 2012 | 22017704 |
| microbiological and chemical characterisation of ting, a sorghum-based sourdough product from botswana. | ting is a fermented sorghum product of botswana which is used to prepare porridges of different consistencies. this study aimed to characterise ting with respect to fermentation microbiota and metabolite composition, and to develop starter cultures for ting fermentation. the ph values of 10 ting samples from botswana ranged between 3.5 and 4.0 and cell counts ranged between 1.2×10(8) and 1.2×10(10)cfu/g lactic acid bacteria. yeast cell counts were below 10(5)cfu/g in all samples and enterobacter ... | 2011 | 21862164 |
| Human Milk Probiotic Lactobacillus fermentum CECT5716 Reduces the Incidence of Gastrointestinal and Upper Respiratory Tract Infections in Infants. | : The aim of the study was to examine the effects of a follow-on formula containing Lactobacillus fermentum CECT5716 (L fermentum) on the incidence of infections in infants between the ages of 6 and 12 months. | 2012 | 21873895 |
| Dietary Supplementation with the Probiotic Lactobacillus fermentum I5007 and the Antibiotic Aureomycin Differentially Affects the Small Intestinal Proteomes of Weanling Piglets. | Antibiotics have long been used in animal production and medication to alleviate weaning stress. However, due to the concerns over food safety and human health, its use in animal production has been prohibited in many countries. Therefore, there is growing interest in developing alternative additives, such as a probiotic Lactobacillus. In this study, a proteomic approach coupled with biochemical analysis was applied to investigate alterations of proteomes in the small intestinal mucosa of weanli ... | 2012 | 22113866 |
| β-galactosidase with transgalactosylation activity from lactobacillus fermentum k4. | the laclm β-galactosidase of lactobacillus fermentum k4 is encoded by 2 consecutive genes, lacl (large subunit) and lacm (small subunit), that share 17 overlapping nucleotides. phylogenetic analysis revealed that this enzyme was closely related to other lactobacillus β-galactosidases and provided significant insight into its common and distinct characteristics. we cloned both the lacl and lacm genes of l. fermentum k4 and heterologously expressed each in escherichia coli, although the recombinan ... | 2011 | 22118071 |
| competitive selection of lactic acid bacteria that persist in the human oral cavity. | lactic acid bacteria (lab) might offer opportunities as oral probiotics provided candidate strains persist in the mouth. after intake of a mixture of 69 lab, strains of lactobacillus fermentum and lactobacillus salivarius were especially recovered. coaggregation with other microbes is likely not a prerequisite for persistence since l. salivarius strongly coaggregated with typical oral cavity isolates, whereas l. fermentum failed to display this phenotype. | 2011 | 21965405 |
| assessment of bacterial diversity in selected philippine fermented food products through pcr-dgge. | the bacterial population in several philippine fermented food preparations was assessed by pcr-denaturing gradient gel electrophoresis (pcr-dgge) of the 16s rrna gene (16s rdna). genomic dna was isolated directly from alamang (fermented shrimp paste), burong isda (fermented fish and rice), burong hipon (fermented shrimp and rice), burong mustasa (fermented mustard leaves), tuba (sugar cane wine), suka (vinegar) and sinamak (spiced vinegar) using one of two protocols, namely - mobio dna extractio ... | 2011 | 22146687 |
| mannitol production by heterofermentative lactobacillus reuteri crl 1101 and lactobacillus fermentum crl 573 in free and controlled ph batch fermentations. | certain lactic acid bacteria, especially heterofermentative strains, are capable to produce mannitol under adequate culture conditions. in this study, mannitol production by lactobacillus reuteri crl 1101 and lactobacillus fermentum crl 573 in modified mrs medium containing a mixture of fructose and glucose in a 6.5:1.0 ratio was investigated during batch fermentations with free ph and constant ph 6.0 and 5.0. mannitol production and yields were higher under constant ph conditions compared with ... | 2011 | 21993480 |
| Oral and faecal lactobacilli and their expression of mannose-specific adhesins in individuals with and without IgA deficiency. | Lactobacilli are present in the intestine and oral cavity of most adults. Secretory IgA in mucosal secretions may provide carbohydrate receptors for bacterial adhesins. Here, oral and faecal samples from 33 IgA-deficient individuals and 34 controls were cultured for lactobacilli, which were identified using species-specific PCR or partial 16S rDNA sequencing and tested for expression of mannose-specific adhesins. Lactobacilli were found in the oral cavity of 76% of IgA-deficient and 85% of contr ... | 2012 | 22018813 |
| production of cured meat color in nitrite-free harbin red sausage by lactobacillus fermentum fermentation. | lactobacillus fermentum was substituted for nitrite to produce cured pink color in a chinese-style sausage. treatments included inoculations (10(4), 10(6), and 10(8)cfu/g meat) followed by fermentation at 30°c for 8h and then at 4°c for 16h. control sausage (with sodium nitrite, 60mg/kg meat) was cured at 4°c for 24h without l. fermentum. the uv-vis spectra of pigment extract from l. fermentum-treated sausage were identical to that of nitrosylmyoglobin (no-mb) formed in nitrite-treated control. ... | 2007 | 22061946 |
| Subcellular localization of N-deoxyribosyltransferase in Lactobacillus fermentum: cell surface association of an intracellular nucleotide metabolic enzyme. | N-deoxyribosyltransferases are essential enzymes in the nucleotide salvage pathway of lactobacilli. They catalyze the exchange between the purine or pyrimidine bases of 2'-deoxyribonucleosides and free pyrimidine or purine bases. In general, N-deoxyribosyltransferases are referred to as cytoplasmic enzymes, although there is no experimental evidence for this subcellular localization. In this work, the subcellular localization of N-deoxyribosyltransferase II (NTD) from Lactobacillus fermentum was ... | 2011 | 22092712 |
| species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional ecuadorian spontaneous cocoa bean fermentations. | traditional fermentations of the local ecuadorian cocoa type nacional, with its fine flavor, are carried out in boxes and on platforms for a short time. a multiphasic approach, encompassing culture-dependent and -independent microbiological analyses of fermenting cocoa pulp-bean samples, metabolite target analyses of both cocoa pulp and beans, and sensory analysis of chocolates produced from the respective fermented dry beans, was applied for the investigation of the influence of these fermentat ... | 2011 | 21926224 |
| antimicrobial and safety properties of lactobacilli isolated from two cameroonian traditional fermented foods. | twenty-one lactobacillus isolates from "sha'a" (a maize - based fermented beverage) and "kossam" (traditionally fermented cow milk) were selected in accordance with their antagonistic activities and tested for their bacteriocinogenic potential as well as safety properties. these isolates were preliminarily identified as lactobacillus plantarum (62%), lactobacillus rhamnosus (24%), lactobacillus fermentum (10%) and lactobacillus coprophilus (4%) based on phenotypic characteristics and rep-pcr gen ... | 2011 | 22396914 |
| on-farm implementation of a starter culture for improved cocoa bean fermentation and its influence on the flavour of chocolates produced thereof. | cocoa bean fermentations controlled by means of starter cultures were introduced on several farms in two different cocoa-producing regions (west africa and southeast asia). two starter culture mixtures were tested, namely one composed of saccharomyces cerevisiae h5s5k23, lactobacillus fermentum 222, and acetobacter pasteurianus 386b (three heaps and one box), and another composed of l. fermentum 222 and a. pasteurianus 386b (seven heaps and one box). in all starter culture-added cocoa bean ferme ... | 2011 | 22365351 |
| acid production and growth by oral lactobacillus species in vitro. | to analyze the acid-producing and growth abilities of different oral lactobacillus species. | 2011 | 22298522 |
| taxonomic and molecular characterization of lactic acid bacteria and yeasts in nunu, a ghanaian fermented milk product. | produced from raw unpasteurized milk, nunu is a spontaneously fermented yoghurt-like product made in ghana and other parts of west africa. despite the importance of nunu in the diet of many africans, there is currently only limited information available on the microorganisms associated with nunu processing. with the aim of obtaining a deeper understanding of the process and as a first step towards developing starter cultures with desired technological properties for nunu production, a microbiolo ... | 2012 | 23541194 |
| development of a multiplex real time pcr to detect thermophilic lactic acid bacteria in natural whey starters. | a multiplex real time pcr (mrealt-pcr) useful to rapidly screen microbial composition of thermophilic starter cultures for hard cooked cheeses and to compare samples with potentially different technological properties was developed. novel primers directed toward phes gene were designed and optimized for multiple detection of lactobacillus helveticus, lactobacillus delbrueckii, streptococcus thermophilus and lactobacillus fermentum. the assay was based on sybr green chemistry followed by melting ... | 2012 | 23290237 |
| spontaneous cocoa bean fermentation carried out in a novel-design stainless steel tank: influence on the dynamics of microbial populations and physical-chemical properties. | spontaneous cocoa bean fermentations carried out in a novel-design 40-kg-capacity stainless steel tank (sst) was studied in parallel to traditional brazilian methods of fermentation in wooden boxes (40-kg-capacity wooden boxes (wb1) and 600-kg-capacity wooden boxes (wb2)) using a multiphasic approach that entailed culture-dependent and -independent microbiological analyses of fermenting cocoa bean pulp samples and target metabolite analyses of both cocoa pulp and cotyledons. both microbiological ... | 2012 | 23279821 |
| the application of genetics methods to differentiation of three lactobacillus species of human origin. | in recent decades, the interest in probiotics as diet supplements or drugs has increased. in order to determine a specific bacterial isolate to be probiotic, it is necessary to describe precisely its probiotic characteristics and taxonomic properties, including the strain level. most of the well-known genotyping methods were designed for the commonly-found pathogenic bacteria. the objective of this study is to undertake an attempt at standardization of fish, rapd and pfge methods to genotype and ... | 2011 | 23144638 |
| bugs that debugs: probiotics. | the oral cavity harbors a diverse array of bacterial species. there are more than 600 species that colonize in the oral cavity. these include a lot of organisms that are not commonly known to reside in the gastrointestinal (gi) tract and also are more familiar: lactobacillus acidophilus, lactobacillus casei, lactobacillus fermentum, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus salivarius. the balance of all these microorganisms can easily be disturbed and a prevalence of p ... | 2012 | 23066281 |
| characterization of bacterial isolates from the microbiota of mothers' breast milk and their infants. | this investigation assessed the potential of isolating novel probiotics from mothers and their infants. a subset of 21 isolates among 126 unique bacteria from breast milk and infant stools from 15 mother-infant pairs were examined for simulated gi transit survival, adherence to caco-2 cells, bacteriocin production, and lack of antibiotic resistance. of the 21 selected isolates a lactobacillus crispatus isolate and 3 lactobacillus gasseri isolates demonstrated good profiles of in vitro gi transit ... | 2015 | 26727418 |
| factors that influence the immunological adjuvant effect of lactobacillus fermentum pc1 on specific immune responses in mice to orally administered antigens. | this study examined the influences of the dosage of the adjuvant, the nature of the antigen and the host genetics on the capacity of l. fermentum pc1 (pc1) to function as an oral adjuvant. balb/c and dba/1 mice were vaccinated with either ovalbumin (ova) or salmonella typhimurium on days 0 and 14, mice were also dosed with the pc1 (10⁸ cfu or 10(11) cfu per dose per mouse) with the antigens (days 0 and 14) and alone (days -1 and 13). the higher pc1 dose elicited a greater specific serum igg2a re ... | 2016 | 27447674 |
| the effect of a novel low temperature-short time (ltst) process to extend the shelf-life of fluid milk. | pasteurization has long been the standard method to extend the shelf-life of dairy products, as well as a means to reduce microbial load and the risk of food-borne pathogens. however, the process has limitations, which include cost effectiveness, high energy input, and reduction of product quality/organoleptic characteristics. in an effort to reduce these limitations and extend shelf-life, this study examined a novel low temperature, short time (ltst) method in which dispersed milk in the form o ... | 2016 | 27350902 |
| phenotypic and genotypic characterization of some lactic acid bacteria isolated from bee pollen: a preliminary study. | in the present work, five hundred and sixty-seven isolates of lactic acid bacteria were recovered from raw bee pollen grains. all isolates were screened for their antagonistic activity against both gram-positive and gram-negative pathogenic bacteria. neutralized supernatants of 54 lactic acid bacteria (lab) cultures from 216 active isolates inhibited the growth of indicator bacteria. they were phenotypically characterized, based on the fermentation of 39 carbohydrates. using the simple matching ... | 2014 | 24936378 |
| enhancement of exopolysaccharide production of lactobacillus fermentum tds030603 by modifying culture conditions. | to optimize culture conditions that enhance production of a highly viscous exopolysaccharide of lactobacillus fermentum tds030603, a chemically defined medium was examined. the best yield was found to be 199 ± 23 mg/l when 48-hr cultivation was microaerobically performed at 30°c in the chemically defined medium supplemented with 5% glucose and 1% ammonium citrate without ph control. in response to the optimized exopolysaccharide production, the mrna expression levels of epsb, epse, and epsg elev ... | 2014 | 25003022 |
| 4,3-α-glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan gh-h. | lactic acid bacteria possess a diversity of glucansucrase (gs) enzymes that belong to glycoside hydrolase family 70 (gh70) and convert sucrose into α-glucan polysaccharides with (α1 → 2)-, (α1 → 3)-, (α1 → 4)- and/or (α1 → 6)-glycosidic bonds. in recent years 3 novel subfamilies of gh70 enzymes, inactive on sucrose but using maltodextrins/starch as substrates, have been established (e.g. gtfb of lactobacillus reuteri 121). compared to the broad linkage specificity found in gss, all gh70 starch-a ... | 2017 | 28059108 |
| phylogenetic analysis of a spontaneous cocoa bean fermentation metagenome reveals new insights into its bacterial and fungal community diversity. | this is the first report on the phylogenetic analysis of the community diversity of a single spontaneous cocoa bean box fermentation sample through a metagenomic approach involving 454 pyrosequencing. several sequence-based and composition-based taxonomic profiling tools were used and evaluated to avoid software-dependent results and their outcome was validated by comparison with previously obtained culture-dependent and culture-independent data. overall, this approach revealed a wider bacterial ... | 2012 | 22666442 |
| detailed analysis of the microbial population in malaysian spontaneous cocoa pulp fermentations reveals a core and variable microbiota. | the fermentation of cocoa pulp is one of the few remaining large-scale spontaneous microbial processes in today's food industry. the microbiota involved in cocoa pulp fermentations is complex and variable, which leads to inconsistent production efficiency and cocoa quality. despite intensive research in the field, a detailed and comprehensive analysis of the microbiota is still lacking, especially for the expanding asian production region. here, we report a large-scale, comprehensive analysis of ... | 2013 | 24358116 |
| oxidation of metabolites highlights the microbial interactions and role of acetobacter pasteurianus during cocoa bean fermentation. | four cocoa-specific acetic acid bacterium (aab) strains, namely, acetobacter pasteurianus 386b, acetobacter ghanensis lmg 23848(t), acetobacter fabarum lmg 24244(t), and acetobacter senegalensis 108b, were analyzed kinetically and metabolically during monoculture laboratory fermentations. a cocoa pulp simulation medium (cpsm) for aab, containing ethanol, lactic acid, and mannitol, was used. all aab strains differed in their ethanol and lactic acid oxidation kinetics, whereby only a. pasteurianus ... | 2014 | 24413595 |
| the impact of yeast starter cultures on the microbial communities and volatile compounds in cocoa fermentation and the resulting sensory attributes of chocolate. | theobroma cacao seeds are the main raw material for chocolate production. during their fermentation, a succession of microorganisms are responsible for the physicochemical changes occurring in the pulp and inside the beans. the aim of this study was to investigate the effects of yeast inoculation (saccharomyces cerevisiae ufla ca11, pichia kluivery ccma0237, and hanseniaspora uvarum ccma0236) on the profile of the volatile compounds and microbial communities in cocoa fermentation. the resulting ... | 2015 | 27162390 |