| grape antioxidant dietary fiber stimulates lactobacillus growth in rat cecum. | practical application: grape antioxidant fiber combines nutritional and physiological properties of dietary fiber and natural antioxidants from grapes. grape antioxidant fiber could be used as an ingredient for functional foods and as a dietary supplement to increase the intake of dietary fiber and bioactive compounds. | 2012 | 22224928 |
| antimicrobial activity of different lactobacillus species against multi- drug resistant clinical isolates of pseudomonas aeruginosa. | lactobacilli are the well known friendly bacteria for their probiotic activities against pathogens. the inhibitory activity of different strains of lactobacilli either obtained as commercial products or isolated from human feces was investigated against the clinical isolates of pseudomonas aeruginosa. the isolates were selected as the most resistant strains when challenged with anti-pseudomonal antibiotics already in clinical practice. | 2011 | 22347578 |
| identification of lactobacillus curvatus tmw 1.624 dextransucrase and comparative characterization with lactobacillus reuteri tmw 1.106 and lactobacillus animalis tmw 1.971 dextransucrases. | recently, it was affirmed that the exopolysaccharides (epss) of lactobacillus curvatus tmw 1.624, lactobacillus reuteri tmw 1.106 and lactobacillus animalis tmw 1.971 improve the quality of gluten-free breads and that they can be produced in situ to levels enabling baking applications. in this study we provide insight into the molecular and biochemical background of eps production of these three strains. eps formation strongly correlated with growth and took place during the exponential phase. g ... | 2012 | 23498178 |
| interaction between lactic acid bacteria and yeasts in airag, an alcoholic fermented milk. | the interaction between nine lactic acid bacteria (lab) and five yeast strains isolated from airag of inner mongolia autonomic region, china was investigated. three representative lab and two yeasts showed symbioses were selected and incubated in 10% (w/v) reconstituted skim milk as single and mixed cultures to measure viable count, titratable acidity, ethanol and sugar content every 24 h for 1 week. lab and yeasts showed high viable counts in the mixed cultures compared to the single cultures. ... | 2012 | 23302085 |
| abnormal weight gain and gut microbiota modifications are side effects of long-term doxycycline and hydroxychloroquine treatment. | doxycycline has been proposed for the treatment of malnourished children in developing countries, and its use has been associated with weight gain in healthy volunteers. no previous studies have assessed abnormal weight gain as a putative side effect of long-term doxycycline treatment; thus, the objective of the present study was to characterize this phenomenon. we also analyzed the role of the gut microbiota in this effect. we assessed changes in the body mass index in q fever endocarditis pati ... | 2014 | 24687497 |
| effect of oral administration involving a probiotic strain of lactobacillus reuteri on pro-inflammatory cytokine response in patients with chronic periodontitis. | this study aimed at evaluation of pro-inflammatory cytokine response (tnf-α, il-1β and il-17) in patients with chronic periodontitis administered per os with a probiotic strain of lactobacillus reuteri. in the 38 adult patients with moderate chronic periodontitis, professional cleaning of teeth was performed. two weeks after performing the oral hygienization procedures, clinical examination permitted to distinguish a group of 24 patients (group 1) in whom treatment with probiotic tablets contain ... | 2014 | 24509697 |
| identification of a proton-chloride antiporter (eric) by himar1 transposon mutagenesis in lactobacillus reuteri and its role in histamine production. | the gut microbiome may modulate intestinal immunity by luminal conversion of dietary amino acids to biologically active signals. the model probiotic organism lactobacillus reuteri atcc pta 6475 is indigenous to the human microbiome, and converts the amino acid l-histidine to the biogenic amine, histamine. histamine suppresses tumor necrosis factor (tnf) production by human myeloid cells and is a product of l-histidine decarboxylation, which is a proton-facilitated reaction. a transposon mutagene ... | 2014 | 24488273 |
| management strategies in the treatment of neonatal and pediatric gastroenteritis. | acute gastroenteritis, characterized by the onset of diarrhea with or without vomiting, continues to be a major cause of morbidity and mortality in children in mostly resource-constrained nations. although generally a mild and self-limiting disease, gastroenteritis is one of the most common causes of hospitalization and is associated with a substantial disease burden. worldwide, up to 40% of children aged less than 5 years with diarrhea are hospitalized with rotavirus. also, some microorganisms ... | 2013 | 24194646 |
| receptor-like molecules on human intestinal epithelial cells interact with an adhesion factor from lactobacillus reuteri. | a surface protein of lactobacillus reuteri, mucus adhesion-promoting protein (mapa), is considered to be an adhesion factor. mapa is expressed in l. reuteri strains and adheres to piglet gastric mucus, collagen type i, and human intestinal epithelial cells such as caco-2. the aim of this study was to identify molecules that mediate the attachment of mapa from l. reuteri to the intestinal epithelial cell surface by investigating the adhesion of mapa to receptor-like molecules on caco-2 cells. map ... | 2012 | 24936355 |
| significant reduction in helicobacter pylori load in humans with non-viable lactobacillus reuteri dsm17648: a pilot study. | reducing the amount of helicobacter pylori in the stomach by selective bacterial-bacterial cell interaction was sought as an effective and novel method for combating the stomach pathogen. lactobacillus reuteri dsm17648 was identified as a highly specific binding antagonist to h. pylori among more than 700 wild-type strains of lactobacillus species. applying a stringent screening procedure, the strain dsm17648 was identified as selective binder to h. pylori cells under in vivo gastric conditions. ... | 2014 | 25481036 |
| oral microbiota shift after 12-week supplementation with lactobacillus reuteri dsm 17938 and pta 5289; a randomized control trial. | lactobacillus spp. potentially contribute to health by modulating bacterial biofilm formation, but their effects on the overall oral microbiota remain unclear. | 2015 | 25946126 |
| dietary gluten-induced gut dysbiosis is accompanied by selective upregulation of micrornas with intestinal tight junction and bacteria-binding motifs in rhesus macaque model of celiac disease. | the composition of the gut microbiome reflects the overall health status of the host. in this study, stool samples representing the gut microbiomes from 6 gluten-sensitive (gs) captive juvenile rhesus macaques were compared with those from 6 healthy, age- and diet-matched peers. a total of 48 samples representing both groups were studied using v4 16s rrna gene dna analysis. samples from gs macaques were further characterized based on type of diet administered: conventional monkey chow, i.e., whe ... | 2016 | 27801835 |
| 4,6-α-glucanotransferase activity occurs more widespread in lactobacillus strains and constitutes a separate gh70 subfamily. | family 70 glycoside hydrolase glucansucrase enzymes exclusively occur in lactic acid bacteria and synthesize a wide range of α-d-glucan (abbreviated as α-glucan) oligo- and polysaccharides. of the 47 characterized gh70 enzymes, 46 use sucrose as glucose donor. a single gh70 enzyme was recently found to be inactive with sucrose and to utilize maltooligosaccharides [(1→4)-α-d-glucooligosaccharides] as glucose donor substrates for α-glucan synthesis, acting as a 4,6-α-glucanotransferase (4,6-αgt) e ... | 2012 | 22361861 |
| chemical modulation of the biological activity of reutericyclin: a membrane-active antibiotic from lactobacillus reuteri. | whilst the development of membrane-active antibiotics is now an attractive therapeutic concept, progress in this area is disadvantaged by poor knowledge of the structure-activity relationship (sar) required for optimizing molecules to selectively target bacteria. this prompted us to explore the sar of the lactobacillus reuteri membrane-active antibiotic reutericyclin, modifying three key positions about its tetramic acid core. the sar revealed that lipophilic analogs were generally more active a ... | 2014 | 24739957 |
| use of atomic force microscopy to study the multi-modular interaction of bacterial adhesins to mucins. | the mucus layer covering the gastrointestinal (gi) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. however, the molecular details of these interactions are not well understood. here, we provide mechanistic insights into the adhesion properties of the canonical mucus-binding protein (mub), a large multi-repeat cell-surface adhesin found in lactobacillus inhabiting the gi tract. we used atomic force microscopy to unravel the mechanism driving mub ... | 2016 | 27834807 |
| global analysis of mannitol 2-dehydrogenase in lactobacillus reuteri crl 1101 during mannitol production through enzymatic, genetic and proteomic approaches. | several plants, fungi, algae, and certain bacteria produce mannitol, a polyol derived from fructose. mannitol has multiple industrial applications in the food, pharmaceutical, and medical industries, being mainly used as a non-metabolizable sweetener in foods. many heterofermentative lactic acid bacteria synthesize mannitol when an alternative electron acceptor such as fructose is present in the medium. in previous work, we reported the ability of lactobacillus reuteri crl 1101 to efficiently pr ... | 2017 | 28060932 |
| exploring optimization parameters to increase ssdna recombineering in lactococcus lactis and lactobacillus reuteri. | single-stranded dna (ssdna) recombineering is a technology which is used to make subtle changes in the chromosome of several bacterial genera. cells which express a single-stranded dna binding protein (rect or bet) are transformed with an oligonucleotide which is incorporated via an annealing and replication-dependent mechanism. by in silico analysis we identified ssdna binding protein homologs in the genus lactobacillus and lactococcus lactis. to assess whether we could further improve the reco ... | 2012 | 22750793 |
| complete genome sequence, lifestyle, and multi-drug resistance of the human pathogen corynebacterium resistens dsm 45100 isolated from blood samples of a leukemia patient. | corynebacterium resistens was initially recovered from human infections and recognized as a new coryneform species that is highly resistant to antimicrobial agents. bacteremia associated with this organism in immunocompromised patients was rapidly fatal as standard minocycline therapies failed. c. resistens dsm 45100 was isolated from a blood culture of samples taken from a patient with acute myelocytic leukemia. the complete genome sequence of c. resistens dsm 45100 was determined by pyrosequen ... | 2012 | 22524407 |
| 4,3-α-glucanotransferase, a novel reaction specificity in glycoside hydrolase family 70 and clan gh-h. | lactic acid bacteria possess a diversity of glucansucrase (gs) enzymes that belong to glycoside hydrolase family 70 (gh70) and convert sucrose into α-glucan polysaccharides with (α1 → 2)-, (α1 → 3)-, (α1 → 4)- and/or (α1 → 6)-glycosidic bonds. in recent years 3 novel subfamilies of gh70 enzymes, inactive on sucrose but using maltodextrins/starch as substrates, have been established (e.g. gtfb of lactobacillus reuteri 121). compared to the broad linkage specificity found in gss, all gh70 starch-a ... | 2017 | 28059108 |
| bio-based 3-hydroxypropionic- and acrylic acid production from biodiesel glycerol via integrated microbial and chemical catalysis. | 3-hydroxypropionic acid (3hp) and acrylic acid (aa) are industrially important platform- and secondary chemical, respectively. their production from renewable resources by environment-friendly processes is desirable. in the present study, both chemicals were almost quantitatively produced from biodiesel-derived glycerol by an integrated process involving microbial and chemical catalysis. | 2015 | 26690945 |
| biochemical characterization of the lactobacillus reuteri glycoside hydrolase family 70 gtfb type of 4,6-α-glucanotransferase enzymes that synthesize soluble dietary starch fibers. | 4,6-α-glucanotransferase (4,6-α-gtase) enzymes, such as gtfb and gtfw of lactobacillus reuteri strains, constitute a new reaction specificity in glycoside hydrolase family 70 (gh70) and are novel enzymes that convert starch or starch hydrolysates into isomalto/maltopolysaccharides (immps). these immps still have linear chains with some α1→4 linkages but mostly (relatively long) linear chains with α1→6 linkages and are soluble dietary starch fibers. 4,6-α-gtase enzymes and their products have sig ... | 2015 | 26253678 |
| identification and characterization of sulfated carbohydrate-binding protein from lactobacillus reuteri. | we previously purified a putative sulfated-galactosylceramide (sulfatide)-binding protein with a molecular weight of 47 kda from the cell surface of lactobacillus reuteri jcm1081. the aim of this study was to identify the 47-kda protein, examine its binding to sulfated glycolipids and mucins, and evaluate its role in bacterial adhesion to mucosal surfaces. by cloning and sequencing analysis, the 47-kda protein was identified as elongation factor-tu (ef-tu). adhesion properties were examined usin ... | 2013 | 24391811 |
| structure of the α-1,6/α-1,4-specific glucansucrase gtfa from lactobacillus reuteri 121. | the reuteransucrase gtfa from lactobacillus reuteri 121, which belongs to glycosyl hydrolase family gh70, synthesizes branched α-glucans with both α-1,6- and α-1,4-glycosidic linkages (reuteran) from sucrose. the crystal structure of gtfa-δn, a 118 kda fragment of gtfa comprising residues 745-1763 and including the catalytic domain, was determined at 3.6 å resolution by molecular replacement. the crystals have large solvent channels and an unusually high solvent content of 85%. gtfa-δn has the s ... | 2012 | 23192022 |
| thiouracil-forming bacteria identified and characterized upon porcine in vitro digestion of brassicaceae feed. | in recent years, the frequent detection of the banned thyreostat thiouracil (tu) in livestock urine has been related to endogenous tu formation following digestion of glucosinolate-rich brassicaceae crops. recently, it was demonstrated that, upon in vitro digestion of brassicaceae, fecal bacteria induce tu detection in livestock (porcine livestock > bovines). therefore, the present study was intended to isolate and identify bacteria involved in this intestinal tu formation upon brassicaceae dige ... | 2014 | 25261511 |
| elucidating and regulating the acetoin production role of microbial functional groups in multispecies acetic acid fermentation. | acetoin (3-hydroxy-2-butanone) formation in vinegar microbiota is crucial for the flavor quality of zhenjiang aromatic vinegar, a traditional vinegar produced from cereals. however, the specific microorganisms responsible for acetoin formation in this centuries-long repeated batch fermentation have not yet been clearly identified. here, the microbial distribution discrepancy in the diacetyl/acetoin metabolic pathway of vinegar microbiota was revealed at the species level by a combination of meta ... | 2016 | 27451452 |
| intestinal microbiota succession and immunomodulatory consequences after introduction of lactobacillus reuteri i5007 in neonatal piglets. | seventy-two, suckling piglets, obtained from 9 litters standardized to 8 piglets, were assigned to 1 of 3 treatments (n = 24) to compare short-term, early administration with intermittent, longer-term administration of lactobacillus reuteri i5007. the treatments were a control (given a placebo of 0.1% peptone water from day 1 to 5) or treatments in which 1.7 × 1010 cfu l. reuteri was administrated either daily for 4 days starting on day 1 or every 4th day from day 1 to 17. five piglets per treat ... | 2015 | 25775260 |
| antimicrobial effect of lactobacillus reuteri on cariogenic bacteria streptococcus gordonii, streptococcus mutans, and periodontal diseases actinomyces naeslundii and tannerella forsythia. | lactic acid bacteria (lab) are well known for their beneficial effects on human health in the intestine and immune system; however, there are few studies on the impact they can generate in oral health. the aim of this study was to test and compare in vitro antimicrobial activity of l. reuteri on pathogenic bacteria involved in the formation of dental caries: s. mutans, s. gordonii, and periodontal disease: a. naeslundii and t. forsythia. also, we determined the growth kinetics of each bacterium ... | 2015 | 25422124 |
| antifungal activity of lactobacilli and its relationship with 3-phenyllactic acid production. | in this study, 13 lactic acid bacteria (lab) strains (including 5 lactobacillus casei, 2 lactobacillus rhamnosus, 2 lactobacillus fermentum, 1 lactobacillus acidophilus, 1 lactobacillus plantarum, 1 lactobacillus sakei, and 1 lactobacillus reuteri species) were assessed for both their antifungal activity against four food spoilage molds (colletotrichum gloeosporioides, botrytis cinerea, penicillium expansum, and aspergillus flavus) and their capability to produce the novel antimicrobial compound ... | 2014 | 24412414 |
| coexpression and secretion of endoglucanase and phytase genes in lactobacillus reuteri. | a multifunctional transgenic lactobacillus with probiotic characteristics and an ability to degrade β-glucan and phytic acid (phytate) was engineered to improve nutrient utilization, increase production performance and decrease digestive diseases in broiler chickens. the bacillus subtilis wl001 endoglucanase gene (celw) and aspergillus fumigatus wl002 phytase gene (phyw) mature peptide (phywm) were cloned into an expression vector with the lactate dehydrogenase promoter of lactobacillus casei an ... | 2014 | 25050780 |
| increased mannitol production in lactobacillus reuteri atcc 55730 production strain with a modified 6-phosphofructo-1-kinase. | based on established knowledge of the simultaneous use of the phosphoketolase pathway (pkp) and the embden-meyerhof pathway (emp) - as a secondary pathway with a smaller flux - by mannitol producer lactobacillus reuteri atcc 55730, we demonstrated the hypothesis that by enhancing the flux through the emp the ability of the microorganism to handle elevated glucose concentrations will be improved, in addition to its growth rate and biomass yield. nadh availability will be increased and its demand ... | 2014 | 24742994 |
| in vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics. | lactic acid bacteria were isolated and identified in the faeces of chinese crested and yorkshire terrier pups and their probiotic features were investigated in vitro. thirty seven isolates were identified as lactobacillus or enterococcus. out of these isolates, 31 were lactic acid bacteria (lab) and belonged to the species lactobacillus reuteri (16/37; 43.3%), lactobacillus animalis (7/37; 18.9%), lactobacillus acidophilus (3/37; 8.1%), lactobacillus sanfranciscensis (2/37; 5.4%), lactobacillus ... | 2013 | 23538205 |
| luminometric label array for counting and differentiation of bacteria. | methods for simple and fast detection and differentiation of bacterial species are required, for instance, in medicine, water quality monitoring, and the food industry. here, we have developed a novel label array method for the counting and differentiation of bacterial species. this method is based on the nonspecific interactions of multiple unstable lanthanide chelates and selected chemicals within the sample leading to a luminescence signal profile that is unique to the bacterial species. it i ... | 2017 | 28194955 |
| possible correlation between levansucrase production and probiotic activity of bacillus sp. isolated from honey and honey bee. | five bacterial isolates from honey and bee gut were selected based on their high levansucrase activity and levan yield which were strongly positively correlated. all isolates showed good tolerance to temperature up to 70 °c, to nacl up to 3 m and to 0.1% h2o2. they maintained over 59 and 64% survival at ph 9.0 and 2.0 respectively, but showed varying tolerance to 0.1% bile salts and pancreatic enzymes. most isolates were susceptible to widely used antibiotics, but demonstrated diverse antimicrob ... | 2017 | 28271385 |
| mixed culture models for predicting intestinal microbial interactions between escherichia coli and lactobacillus in the presence of probiotic bacillus subtilis. | bacillus has been proposed as a probiotic due to its in vivo effectiveness in the gastrointestinal tract through antimicrobial activities. the present study investigates the effects of lactobacillus alone or in the presence of bacillus subtilis ma139 on the inhibition of pathogenic escherichia coli k88. mixed cultures were used to predict the possible interactions among these bacteria within the intestinal tract of animals. b. subtilis ma139 was first assayed for its inhibition against e. coli k ... | 2015 | 26259891 |
| modulation of the intestinal microbiota and morphology of tilapia, oreochromis niloticus, following the application of a multi-species probiotic. | the intestinal microbiota and morphology of tilapia (oreochromis niloticus) were investigated after the application of a multi-species probiotic containing lactobacillus reuteri, bacillus subtilis, enterococcus faecium and pediococcus acidilactici (aquastar(®) growout). tilapia (55.03 ± 0.44 g) were fed either a control diet or a probiotic diet (control diet supplemented with aquastar(®) growout at 5 g kg(-1)). after four and eight weeks, culture-dependent analysis showed higher levels of lactic ... | 2015 | 26115752 |
| effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (oncorhynchus mykiss). | dietary supplementation with a multi-strain probiotic containing bacillus subtilis, enterococcus faecium, pediococcus acidilactici and lactobacillus reuteri has been examined for its effects on growth performance, intestinal microbiota, non-specific immune response and antioxidant status of rainbow trout. three groups of sub-adult trout were stocked into experimental tanks. a commercial diet was used as control, while the other two groups received diets supplemented with the multi-strain probiot ... | 2015 | 25411048 |
| supplementation of direct-fed microbials as an alternative to antibiotic on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. | an experiment was conducted to investigate the supplementation of direct-fed microbials (dfm) as an alternative to antibiotics on growth performance, immune response, cecal microbial population, and ileal morphology of broiler chickens. a total of 800 one-day-old male broiler chicks (ross × ross) were randomly allotted to 4 dietary treatments with 4 replicate pens per treatment (50 birds/replicate pen). the 4 dietary treatments fed for 35 d were a corn-soybean meal basal diet (control); control ... | 2013 | 23873556 |
| enhanced viability of lactobacillus reuteri for probiotics production in mixed solid-state fermentation in the presence of bacillus subtilis. | in order to develop a multi-microbe probiotic preparation of lactobacillus reuteri g8-5 and bacillus subtilis ma139 in solid-state fermentation, a series of parameters were optimized sequentially in shake flask culture. the effect of supplementation of b. subtilis ma139 as starters on the viability of l. reuteri g8-5 was also explored. the results showed that the optimized process was as follows: water content, 50 %; initial ph of diluted molasses, 6.5; inocula volume, 2 %; flask dry contents, 3 ... | 2014 | 23775321 |
| electrotransformation and expression of cellulase genes in wild-type lactobacillus reuteri. | two cellulase genes, cel15 and cel73, were amplified from bacillus subtilis genome dna in a previous study. two integrative vectors, plem4153 and plem4154, containing the genes cel15 and cel73, respectively, were constructed and successfully electroporated into the wild-type lactobacillus reuteri which was isolated from chick guts through an optimized procedure. two recombinant l. reuteri were selected from a man, rogosa, and sharp (mrs) plate with 10 µg/ml erythromycin, and named l. reuteri xny ... | 2012 | 22964542 |
| the role of conserved inulosucrase residues in the reaction and product specificity of lactobacillus reuteri inulosucrase. | the probiotic bacterium lactobacillus reuteri 121 produces two fructosyltransferase enzymes, a levansucrase and an inulosucrase. although these two fructosyltransferase enzymes share high sequence similarity, they differ significantly in the type and size distribution of fructooligosaccharide products synthesized from sucrose, and in their activity levels. in order to examine the contribution of specific amino acids to such differences, 15 single and four multiple inulosucrase mutants were desig ... | 2012 | 22823473 |
| assessment of dietary supplementation with probiotics on performance, intestinal morphology and microflora of chickens infected with eimeria tenella. | we evaluated the effects of dietary supplementation with different preparations of probiotics on the performance of broiler chickens experimentally infected with 2 × 10(4) sporulated oocysts of eimeria tenella at 14 days of age. three hundred, day-old, cobb-500 chicks, as hatched, were separated into 10 equal groups with three replicates. two of the groups, one challenged with e. tenella oocysts and the other not, were given a basal diet and served as controls without medication. the other chall ... | 2012 | 22459110 |
| differential metabolism of exopolysaccharides from probiotic lactobacilli by the human gut symbiont bacteroides thetaiotaomicron. | probiotic microorganisms are ingested as food or supplements and impart positive health benefits to consumers. previous studies have indicated that probiotics transiently reside in the gastrointestinal tract and, in addition to modulating commensal species diversity, increase the expression of genes for carbohydrate metabolism in resident commensal bacterial species. in this study, it is demonstrated that the human gut commensal species bacteroides thetaiotaomicron efficiently metabolizes fructa ... | 2015 | 25841008 |
| isolation and characterization of faecal bifidobacteria and lactobacilli isolated from dogs and primates. | although bifidobacteria and lactobacilli have been suggested beneficial for the host and are components of many probiotics and competitive exclusion mixtures, the knowledge on abundance, metabolic and probiotic characteristics in isolates from dogs and monkeys is still limited. the present study was aimed to isolate bifidobacterium and lactobacillus strains (faeces of 22 dogs and of 5 primates: cebus apella, eulemur fulvus, erythrocebus patas, macaca fascicularis, papio hamadryas) with the maldi ... | 2014 | 24239978 |
| lactobacillus reuteri and escherichia coli in the human gut microbiota may predict weight gain associated with vancomycin treatment. | antibiotics, used for 60 years to promote weight gain in animals, have been linked to obesity in adults and in children when administered during early infancy. lactobacillus reuteri has been linked to obesity and weight gain in children affected with kwashiorkor using ready-to-use therapeutic food. in contrast, escherichia coli has been linked with the absence of obesity. both of these bacteria are resistant to vancomycin. | 2013 | 24018615 |
| effect of probiotics lactobacillus and bifidobacterium on gut-derived lipopolysaccharides and inflammatory cytokines: an in vitro study using a human colonic microbiota model. | gut-derived lipopolysaccharides (lps) are critical to the development and progression of chronic low-grade inflammation and metabolic diseases. in this study, the effects of probiotics lactobacillus and bifidobacterium on gut-derived lipopolysaccharide and inflammatory cytokine concentrations were evaluated using a human colonic microbiota model. lactobacillus reuteri, l. rhamnosus, l. plantarum, bifidobacterium animalis, b. bifidum, b. longum, and b. longum subsp. infantis were identified from ... | 2013 | 23568206 |
| correlation between body mass index and gut concentrations of lactobacillus reuteri, bifidobacterium animalis, methanobrevibacter smithii and escherichia coli. | genus and species level analysis is the best way to characterize alterations in the human gut microbiota that are associated with obesity, because the clustering of obese and lean microbiotas increases with the taxonomic depth of the analysis. bifidobacterium genus members have been associated with a lean status, whereas different lactobacillus species are associated both with a lean and an obese status. | 2013 | 23459324 |
| eugenol in combination with lactic acid bacteria attenuates listeria monocytogenes virulence in vitro and in invertebrate model galleria mellonella. | listeria monocytogenes is a human enteric pathogen that causes severe foodborne illness in high-risk populations. crossing the intestinal barrier is the first critical step for listeria monocytogenes infection. therefore, reducing l. monocytogenes colonization and invasion of intestinal epithelium and production of virulence factors could potentially control listeriosis in humans. this study investigated the efficacy of sub-inhibitory concentration (sic) of the plant-derived antimicrobial eugeno ... | 2016 | 27002648 |
| probiotic administration modifies the milk fatty acid profile, intestinal morphology, and intestinal fatty acid profile of goats. | the effect of a mixture of potentially probiotic bacteria (mppb; lactobacillus reuteri ddl 19, lactobacillus alimentarius ddl 48, enterococcus faecium dde 39, and bifidobacterium bifidum strains) on the milk fatty acid (fa) profile, with emphasis on cis-9,trans-11 conjugated linoleic acid (cla) in the middle stage of goat lactation, was determined. in addition, the effects of mppb feeding on the fa profile in intestinal content and intestinal morphology in weaned goats were analyzed. the probiot ... | 2015 | 25465559 |
| influence of bovine lactoferrin on the growth of selected probiotic bacteria under aerobic conditions. | bovine lactoferrin (blf) is a natural glycoprotein, and it shows broad-spectrum antimicrobial activity. however, reports on the influences of blf on probiotic bacteria have been mixed. we examined the effects of apo-blf (between 0.25 and 128 mg/ml) on both aerobic and anaerobic cultures of probiotics. we found that blf had similar effects on the growth of probiotics under aerobic or anaerobic conditions, and that it actively and significantly (at concentrations of >0.25 mg/ml) retarded the growt ... | 2014 | 24916115 |
| potential of goat probiotic to bind mutagens. | the mutagen binding ability of the goat probiotics (lactobacillus reuteri ddl 19, lactobacillus alimentarius ddl 48, enterococcus faecium dde 39, and bifidobacterium bifidum ddba) was evaluated. the oral administration of these probiotics reduced fecal mutagens and intestinal cancer markers in goats. secondly, the effects of probiotics against the mutagenesis induced by sodium azide (sa), and benzopyrene (b[α]p) by performing the modified ames test using salmonella typhimurium ta 100 was investi ... | 2014 | 24785349 |
| polysaccharide from seeds of plantago asiatica l. affects lipid metabolism and colon microbiota of mouse. | polysaccharide from the seeds of plantago asiatica l. was given via oral administration to mice (0.4 g/kg body weight, 30 days) to observe its effects on mouse nutrient metabolism and colon microbiota. it was found the polysaccharide intake could lower the apparent absorption of lipid. total triglyceride, cholesterol, and atherogenic index in blood serum with total lipid and cholesterol levels in liver of polysaccharide group mice were all significantly lower than those of the control group (p < ... | 2014 | 24341731 |
| role of probiotics in the prevention and treatment of meticillin-resistant staphylococcus aureus infections. | meticillin-resistant staphylococcus aureus (mrsa) is a multidrug-resistant micro-organism and is the principal nosocomial pathogen worldwide. following initial in vitro experiments demonstrating that lactobacillus acidophilus cl1285(®) and lactobacillus casei lbc80r(®) commercial strains exhibit antibacterial activity against clinical mrsa isolates, we conducted a literature search to find any evidence of probiotic efficacy in decolonisation or treatment of s. aureus infection. as summarised bel ... | 2013 | 24071026 |
| antimicrobial potential for the combination of bovine lactoferrin or its hydrolysate with lactoferrin-resistant probiotics against foodborne pathogens. | previous reports have shown that several probiotic strains can resist the antibacterial activity of bovine lactoferrin (blf), but the results are inconsistent. moreover, a portion of orally administered apo-blf is digested in vivo by pepsin to yield blf hydrolysate, which produces stronger antibacterial activity than that observed with apo-blf. however, whether blf hydrolysate affects the growth of probiotic strains is unclear. therefore, various probiotic strains in taiwan were collected and ev ... | 2013 | 23332852 |
| transferase activity of lactobacillal and bifidobacterial β-galactosidases with various sugars as galactosyl acceptors. | the β-galactosidases from lactobacillus reuteri l103 (lreuβgal), lactobacillus delbrueckii subsp. bulgaricus dsm 20081 (lbulβgal), and bifidobacterium breve dsm 20281 (bbreβgal-i and bbreβgal-ii) were investigated in detail with respect to their propensity to transfer galactosyl moieties onto lactose, its hydrolysis products d-glucose and d-galactose, and certain sugar acceptors such as n-acetyl-d-glucosamine (glcnac), n-acetyl-d-galactosamine (galnac), and l-fucose (fuc) under defined, initial ... | 2016 | 26975338 |
| protection of probiotic bacteria in a synbiotic matrix following aerobic storage at 4 °c. | the survival of single strains of bifidobacterium breve, bifidobacterium longum, lactobacillus acidophilus, and lactobacillus reuteri was investigated in synbiotics that included 10 mg/ml of fructo-oligosaccharides, inulin and pectic-oligosaccharides in an alginate matrix under refrigerated (4 °c) aerobic storage conditions. when the matrices were cross-linked with calcium (45 mm), 102-103 cfu/ml of l. acidophilus and l. reuteri, and 0-103 cfu/ml of b. breve and b. longum survived refrigerated a ... | 2012 | 22968408 |
| safety and acceptability of lactobacillus reuteri dsm 17938 and bifidobacterium longum subspecies infantis 35624 in bangladeshi infants: a phase i randomized clinical trial. | probiotics have rarely been studied in young healthy infants from low-income countries. this phase i study investigated the safety and acceptability of two probiotics in bangladesh. | 2016 | 26832746 |
| effect of traditional leafy vegetables on the growth of lactobacilli and bifidobacteria. | traditional leafy vegetables, apart from being a staple in the diet of most of sub-saharan africa, are an essential part of traditional medicine and are used daily by traditional healers in the region to treat a wide variety of ailments. in this study, a batch culture technique was used to investigate whether 25 infusions from 22 traditional leafy vegetables stimulated the growth of lactobacillus bulgaricus, lactobacillus lactis, lactobacillus reuteri and bifidobacterium longum in pure culture. ... | 2014 | 25088723 |
| oral treatment of chickens with lactobacillus reuteri lm1 reduces brachyspira pilosicoli-induced pathology. | avian intestinal spirochaetosis (ais) results from the colonization of the caeca and colon of poultry by pathogenic brachyspira, notably brachyspira pilosicoli. following the ban on the use of antibiotic growth promoters in the european union in 2006, the number of cases of ais has increased, which, alongside emerging antimicrobial resistance in brachyspira, has driven renewed interest in alternative intervention strategies. lactobacillus-based probiotics have been shown to protect against infec ... | 2013 | 23161764 |
| lactobacillus reuteri atcc55730 in cystic fibrosis. | the aim of this study was to evaluate in patients with cystic fibrosis (cf) the effect of lactobacillus reuteri (lr) on the rate of respiratory exacerbations and of the infections of both upper respiratory and gastrointestinal tracts. | 2014 | 24121143 |
| screening for lactic acid bacteria capable of inhibiting campylobacter jejuni in in vitro simulations of the broiler chicken caecal environment. | thermotolerant campylobacter spp., specifically campylobacter jejuni and campylobacter coli, are the most common bacterial causes of human gastroenteritis in developed countries. consumption of improperly prepared poultry products and cross contamination are among the main causes of human campylobacteriosis. the aim of this study was to identify lactic acid bacterial (lab) strains capable of inhibiting c. jejuni growth in initial in vitro trials ('spot-on-lawn' method), as well as in batch ferme ... | 2012 | 23234730 |
| evaluating the efficacy of an avian-specific probiotic to reduce the colonization of campylobacter jejuni in broiler chickens. | campylobacteriosis is the most frequent zoonotic disease in humans worldwide, and the contaminated poultry meat by campylobacter jejuni can be considered one of the important sources of enteric infections in humans. the use of probiotics, which can help to improve the natural defense of animals against pathogenic bacteria, is an alternative and effective approach to antibiotic administration for livestock to reduce bacterial contamination. in vitro experiments showed that enterococcus faecium, p ... | 2012 | 22802174 |
| probiotic lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 exhibit strong antifungal effects against vulvovaginal candidiasis-causing candida glabrata isolates. | this study investigates the antagonistic effects of the probiotic strains lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 against vulvovaginal candidiasis (vvc)-causing candida glabrata. | 2015 | 25688886 |
| inhibitory activity in vitro of probiotic lactobacilli against oral candida under different fermentation conditions. | clinical studies have shown that probiotics positively affect oral health by decreasing gum bleeding and/or reducing salivary counts of certain oral pathogens. our aim was to investigate the inhibitory effect of six probiotic lactobacilli against opportunistic oral candida species. sugar utilisation by both lactobacilli and candida was also assessed. agar overlay assay was utilised to study growth inhibition of candida albicans, candida glabrata and candida krusei by lactobacillus rhamnosus gg, ... | 2015 | 25380800 |
| in vitro and in vivo effects of beneficial vaginal lactobacilli on pathogens responsible for urogenital tract infections. | the aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (lb) on urogenital pathogens through in vitro and in vivo experiments. co-aggregative and antimicrobial properties between five vaginal lb strains and urogenital pathogens or potential pathogens (streptococcus agalactiae, staphylococcus aureus and candida albicans strains) were assayed. also, associative cultures of lb strains and s. agalactiae were performed and bacterial growth, ph, lactic acid and hydrog ... | 2014 | 24523160 |
| probiotic interference of lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 with the opportunistic fungal pathogen candida albicans. | candida albicans is the most important candida species causing vulvovaginal candidiasis (vvc). vvc has significant medical and economical impact on women's health and wellbeing. while current antifungal treatment is reasonably effective, supportive and preventive measures such as application of probiotics are required to reduce the incidence of vvc. we investigated the potential of the probiotics lactobacillus rhamnosus gr-1 and lactobacillus reuteri rc-14 towards control of c. albicans. in vitr ... | 2012 | 22811591 |
| probiotic lactobacillus and estrogen effects on vaginal epithelial gene expression responses to candida albicans. | vaginal epithelial cells have receptors, signal transduction mechanisms, and cytokine secretion capabilities to recruit host defenses against candida albicans infections. this research evaluates how probiotic lactobacilli affect the defensive epithelial response. | 2012 | 22715972 |
| identification and biological activity of potential probiotic bacterium isolated from the stomach mucus of breast-fed lamb. | the lactic acid bacterium e isolated from the stomach mucus of breast-fed lamb was identified by sequencing of 16s rdna fragment and species-specific pcr as lactobacillus reuteri. its potential antimicrobial activity and ability to modulate immune system in vitro and in vivo was determined. the growth inhibition of potential pathogens decreased from staphylococcus aureus, pseudomonas aeruginosa, salmonella enterica ser. minnesota to escherichia coli. the lowest inhibition activity was observed i ... | 2011 | 24031741 |
| in vitro modulation of probiotic bacteria on the biofilm of candida glabrata. | a conspicuous new concept of pathogens living as the microbial societies in the human host rather than free planktonic cells has raised considerable concerns among scientists and clinicians. fungal biofilms are communities of cells that possess distinct characteristic such as increased resistance to the immune defence and antimycotic agents in comparison to their planktonic cells counterpart. therefore, inhibition of the biofilm may represent a new paradigm for antifungal development. in this st ... | 2015 | 26028405 |
| stressor exposure has prolonged effects on colonic microbial community structure in citrobacter rodentium-challenged mice. | stressor exposure significantly affects the colonic mucosa-associated microbiota, and exacerbates citrobacter rodentium-induced inflammation, effects that can be attenuated with probiotic lactobacillus reuteri. this study assessed the structure of the colonic mucosa-associated microbiota in mice exposed to a social stressor (called social disruption), as well as non-stressed control mice, during challenge with the colonic pathogen c. rodentium. mice were exposed to the social stressor or home ca ... | 2017 | 28344333 |
| social stress-enhanced severity of citrobacter rodentium-induced colitis is ccl2-dependent and attenuated by probiotic lactobacillus reuteri. | psychological stressors are known to affect colonic diseases but the mechanisms by which this occurs, and whether probiotics can prevent stressor effects, are not understood. because inflammatory monocytes that traffic into the colon can exacerbate colitis, we tested whether ccl2, a chemokine involved in monocyte recruitment, was necessary for stressor-induced exacerbation of infectious colitis. mice were exposed to a social disruption stressor that entails repeated social defeat. during stresso ... | 2016 | 26422754 |
| probiotic lactobacillus reuteri attenuates the stressor-enhanced severity of citrobacter rodentium infection. | stressor exposure has been shown to enhance host susceptibility and the severity of a plethora of illnesses, including gastrointestinal disease. in mice, susceptibility to citrobacter rodentium has been shown to be dependent on host genetics as well as the composition of the intestinal microbiota, but the effects of stressor exposure on this gastrointestinal pathogen have not been elucidated fully. previously, our lab showed that exposure to the prolonged-restraint stressor prior to a challenge ... | 2013 | 23798531 |
| lactobacillus reuteri dsm 17938 in the prevention of antibiotic-associated diarrhoea in children: protocol of a randomised controlled trial. | administration of some probiotics appears to reduce the risk of antibiotic-associated diarrhoea (aad). the effects of probiotics are strain-specific, thus, the efficacy and safety of each probiotic strain should be established separately. we aim to assess the effects of lactobacillus reuteri dsm 17938 administration for the prevention of diarrhoea and aad in children. | 2017 | 28057659 |
| gastrointestinal localization of metronidazole by a lactobacilli-inspired tetramic acid motif improves treatment outcomes in the hamster model of clostridium difficile infection. | metronidazole, a mainstay treatment for clostridium difficile infection (cdi), is often ineffective for severe cdi. whilst this is thought to arise from suboptimal levels of metronidazole in the colon due to rapid absorption, empirical validation is lacking. in contrast, reutericyclin, an antibacterial tetramic acid from lactobacillus reuteri, concentrates in the gastrointestinal tract. in this study, we modified metronidazole with reutericyclin's tetramic acid motif to obtain non-absorbed compo ... | 2015 | 26286574 |
| a novel method for screening of potential probiotics for high adhesion capability. | to screen for potential probiotics with high adhesion capability, a chemostat model-based cultured human feces and denaturing gradient gel electrophoresis methods were applied, and the adhesion capability of the isolates was evaluated in vitro and in vivo. lactobacillus plantarum (hm218749), lactobacillus reuteri (eu547310), and enterococcus faecalis (hm218543) were isolated from the slime on the chemostat wall, as these organisms could grow better at 37°c in an anaerobic environment and could r ... | 2015 | 25912863 |
| probiotics and gut health in infants: a preliminary case-control observational study about early treatment with lactobacillus reuteri dsm 17938. | we performed this case-control observational study to evaluate the effects of early administration of lactobacillus reuteri dsm 17938 on microbial composition in infants' gastrointestinal tract. | 2015 | 25704298 |
| effect of lactobacillus reuteri on the proliferation of propionibacterium acnes and staphylococcus epidermidis. | while it is generally accepted that propionibacterium acnes is involved in the development of acne, other bacteria including staphylococcus epidermidis have also been isolated from the acne lesion. the interaction between lactobacillus reuteri, a probiotic bacterium, and acnegenic bacteria is unclear. this study examined the effects of l. reuteri on the proliferation of p. acnes and s. epidermidis. human-derived l. reuteri strains (kctc 3594 and kctc 3678) and rat-derived l. reuteri kctc 3679 we ... | 2012 | 22367948 |
| animal rennets as sources of dairy lactic acid bacteria. | the microbial composition of artisan and industrial animal rennet pastes was studied by using both culture-dependent and -independent approaches. pyrosequencing targeting the 16s rrna gene allowed to identify 361 operational taxonomic units (otus) to the genus/species level. among lactic acid bacteria (lab), streptococcus thermophilus and some lactobacilli, mainly lactobacillus crispatus and lactobacillus reuteri, were the most abundant species, with differences among the samples. twelve groups ... | 2014 | 24441167 |
| presence of erythromycin and tetracycline resistance genes in lactic acid bacteria from fermented foods of indian origin. | lactic acid bacteria (lab) resistant to erythromycin were isolated from different food samples on selective media. the isolates were identified as enterococcus durans, enterococcus faecium, enterococcus lactis, enterococcus casseliflavus, lactobacillus salivarius, lactobacillus reuteri, lactobacillus plantarum, lactobacillus fermentum, pediococcus pentosaceus and leuconostoc mesenteroides. of the total 60 isolates, 88 % harbored the ermb gene. the efflux gene msra was identified in e. faecium, e ... | 2012 | 22644346 |
| biofilm-forming capacity in biogenic amine-producing bacteria isolated from dairy products. | biofilms on the surface of food industry equipment are reservoirs of potentially food-contaminating bacteria-both spoilage and pathogenic. however, the capacity of biogenic amine (ba)-producers to form biofilms has remained largely unexamined. bas are low molecular weight, biologically active compounds that in food can reach concentrations high enough to be a toxicological hazard. fermented foods, especially some types of cheese, accumulate the highest ba concentrations of all. the present work ... | 2016 | 27242675 |
| antagonistic activity of lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. | adhesion ability of probiotics is the key factor that decides their colonization in the gastrointestinal tract and potential to inhibit pathogens. therefore, adhesion ability can be considered as a key determinant for probiotic efficacy. presents study documents the antagonistic activity of viable/untreated, lithium chloride (licl) treated or heat-killed forms of eight probiotic lactobacillus reuteri strains on the adhesion characteristics of selected pathogens. all strains investigated were abl ... | 2017 | 28377765 |
| effects of the probiotic enterococcus faecium ncimb 10415 on selected lactic acid bacteria and enterobacteria in co-culture. | enterococcus faecium ncimb 10415 is used as a probiotic for piglets and has been shown to modify the porcine intestinal microbiota. however, the mode of action of this probiotic modification is still unclear. one possible explanation is the direct growth inhibiting or stimulating effect of the probiotic on other indigenous bacteria. therefore, the aim of the present study was to examine the growth interactions of the probiotic with different indigenous porcine bacteria in vitro. reference strain ... | 2015 | 25519527 |
| gassericin a: a circular bacteriocin produced by lactic acid bacteria lactobacillus gasseri. | during the recent years extensive efforts have been made to find out bacteriocins from lactic acid bacteria (lab) active against various food spoilage and pathogenic bacteria, and superior stabilities against heat treatments and ph variations. bacteriocins isolated from lab have been grouped into four classes. circular bacteriocins which were earlier grouped among the four groups of bacteriocins, have recently been proposed to be classified into a different class, making it class v bacteriocins. ... | 2013 | 23712477 |
| effect of probiotics on biomarkers of cardiovascular disease: implications for heart-healthy diets. | the objectives of this article are to review clinical trials that have examined the effects of probiotics on low-density lipoprotein cholesterol (ldl-c) and to assess the potential of probiotic intake as a therapeutic lifestyle change (tlc) dietary option. twenty-six clinical studies and two meta-analyses are reviewed. significant ldl-c reductions were observed for four probiotic strains: lactobacillus reuteri ncimb 30242, enterococcus faecium, and the combination of lactobacillus acidophilus la ... | 2014 | 24330093 |
| dietary inclusion of feathers affects intestinal microbiota and microbial metabolites in growing leghorn-type chickens. | feather pecking in laying hens is a serious behavioral problem that is often associated with feather eating. the intake of feathers may influence the gut microbiota and its metabolism. the aim of this study was to determine the effect of 2 different diets, with or without 5% ground feathers, on the gut microbiota and the resulting microbial fermentation products and to identify keratin-degrading bacteria in chicken digesta. one-day-old lohmann-selected leghorn chicks were divided into 3 feeding ... | 2012 | 22700493 |
| erratum to: antiviral activity of lactobacillus reuteri protectis against coxsackievirus a and enterovirus 71 infection in human skeletal muscle and colon cell lines. | | 2016 | 27855715 |
| antiviral activity of lactobacillus reuteri protectis against coxsackievirus a and enterovirus 71 infection in human skeletal muscle and colon cell lines. | recurrence of hand, foot and mouth disease (hfmd) pandemics continues to threaten public health. despite increasing awareness and efforts, effective vaccine and drug treatment have yet to be available. probiotics have gained recognition in the field of healthcare worldwide, and have been extensively prescribed to babies and young children to relieve gastrointestinal (gi) disturbances and diseases, associated or not with microbial infections. since the faecal-oral axis represents the major route ... | 2016 | 27341804 |
| production of reuterin in a fermented milk product by lactobacillus reuteri: inhibition of pathogens, spoilage microorganisms, and lactic acid bacteria. | we assessed the antimicrobial activity of reuterin produced in vitro in glycerol aqueous solutions in situ by lactobacillus reuteri atcc 53608 as part of a fermented milk product against starter (lactobacillus delbrueckii ssp. bulgaricus and streptococcus thermophilus), spoilage (penicillium expansum), pathogenic (staphylococcus aureus, salmonella enterica ssp. enterica, and listeria monocytogenes), and pathogen surrogate (escherichia coli dh5α) microorganisms. we also assayed the influence of c ... | 2017 | 28342608 |
| in vitro effect of vaginal lactobacilli on the growth and adhesion abilities of uropathogenic escherichia coli. | escherichia coli is one of the main causes of uncomplicated urinary tract infections and responsible of vaginal infections. lactobacilli can inhibit this pathogen by the production of antimicrobial substances as organic acids, hydrogen peroxide and/or bacteriocins. the aim of this work was to study the effects of beneficial vaginal lactobacilli on e. coli through in vitro experiments. the inhibitory activity of three vaginal lactobacillus strains against e. coli was assessed using the agar plate ... | 2017 | 28280842 |
| coproduction of colicin v and lactic acid bacteria bacteriocins in lactococci and enterococci strains of biotechnological interest. | the aim of this study was the coproduction in a single strain of the gram-negative bacteriocin colicin v with other bacteriocins from lactic acid bacteria (lab). | 2017 | 28261897 |
| strain-dependent augmentation of tight-junction barrier function in human primary epidermal keratinocytes by lactobacillus and bifidobacterium lysates. | in this study, we investigated whether probiotic lysates can modify the tight-junction function of human primary keratinocytes. the keratinocytes were grown on cell culture inserts and treated with lysates from bifidobacterium longum, lactobacillus plantarum, lactobacillus reuteri, lactobacillus fermentum, or lactobacillus rhamnosus gg. with the exception of l. fermentum (which decreased cell viability), all strains markedly enhanced tight-junction barrier function within 24 h, as assessed by me ... | 2013 | 23770906 |
| ultrasound attenuation of lactobacilli and bifidobacteria: effect on some technological and probiotic properties. | attenuation can be regarded as a tool to modulate the metabolism of probiotic bacteria and, consequently, a strategy to reduce the acidification of the active drinks. attenuation can be done through chemical and physical approaches and ultrasound (us) is a possibility, previously tested to modulate the metabolism of lactic acid bacteria inoculated in a rice drink, but no data are available on the effect of this treatment of the overall profile of probiotic bacteria. therefore, the main topic of ... | 2017 | 28038333 |
| hypocholesterolemic effects of probiotic mixture on diet-induced hypercholesterolemic rats. | growing evidence has indicated that supplementation with probiotics improves lipid metabolism. we aimed to investigate the beneficial effects of a probiotics mixture (pm) of three strains belonging to the species bifidobacterium (b. longum, b. lactis, and b. breve) and two strains belonging to the species lactobacillus (l. reuteri and l. plantarum) on cholesterol-lowering efficacy in hypercholesterolemic rats. a hypercholesterolemic rat model was established by feeding a high-cholesterol diet fo ... | 2017 | 28300786 |
| orally administered multispecies probiotic formulations to prevent uro-genital infections: a randomized placebo-controlled pilot study. | the aim of this study was to evaluate in the vagina of 60 pre-menopausal women the detection of orally administered multispecies probiotic formulations showing anti-microbial properties in test in vitro. | 2017 | 27826653 |
| benzoic acid production with respect to starter culture and incubation temperature during yogurt fermentation using response surface methodology. | benzoic acid is occasionally used as a raw material supplement in food products and is sometimes generated during the fermentation process. in this study, the production of naturally occurring yogurt preservatives was investigated for various starter cultures and incubation temperatures, and considered food regulations. streptococcus thermophilus, lactobacillus acidophilus, lactobacillus delbrueckii subsp. bulgaricus, lactobacillus rhamnosus, lactobacillus casei, lactobacillus paracasei, lactoba ... | 2016 | 27433115 |
| genetic determinants of reutericyclin biosynthesis in lactobacillus reuteri. | reutericyclin is a unique antimicrobial tetramic acid produced by some strains of lactobacillus reuteri. this study aimed to identify the genetic determinants of reutericyclin biosynthesis. comparisons of the genomes of reutericyclin-producing l. reuteri strains with those of non-reutericyclin-producing strains identified a genomic island of 14 open reading frames (orfs) including genes coding for a nonribosomal peptide synthetase (nrps), a polyketide synthase (pks), homologues of phla, phlb, an ... | 2015 | 25576609 |
| probiotic microorganisms inhibit epithelial cell internalization of botulinum neurotoxin serotype a. | botulinum neurotoxins (bonts) are some of the most poisonous natural toxins known to man and are threats to public health and safety. previous work from our laboratory showed that both bont serotype a complex and holotoxin can bind and transit through the intestinal epithelia to disseminate in the blood. the timing of bont/a toxin internalization was shown to be comparable in both the caco-2 in vitro cell culture and in the oral mouse intoxication models. probiotic microorganisms have been exten ... | 2016 | 27999281 |
| a combination of lactic acid bacteria regulates escherichia coli infection and inflammation of the bovine endometrium. | uterine function in cattle is compromised by bacterial contamination and inflammation after calving. the objective of this study was to select a combination of lactic acid bacteria (lab) to decrease endometrium inflammation and escherichia coli infection. primary endometrial epithelial cells were cultured in vitro to select the most favorable lab combination modulating basal tissue inflammation and e. coli infection. supernatants were obtained to determine expression of pro-inflammatory cytokine ... | 2017 | 27837977 |
| horizontal transfer of the salmonella enterica serovar infantis resistance and virulence plasmid pesi to the gut microbiota of warm-blooded hosts. | salmonella enterica serovar infantis is one of the prevalent salmonellae worldwide. recently, we showed that the emergence of s infantis in israel was facilitated by the acquisition of a unique megaplasmid (pesi) conferring multidrug resistance and increased virulence phenotypes. here we elucidate the ecology, transmission properties, and regulation of pesi. we show that despite its large size (~280 kb), pesi does not impose a significant metabolic burden in vitro and that it has been recently f ... | 2016 | 27601577 |
| structure-function relationships of bacterial and enzymatically produced reuterans and dextran in sourdough bread baking application. | exopolysaccharides from lactic acid bacteria may improve texture and shelf life of bread. the effect of exopolysaccharides on bread quality, however, depends on properties of the eps and the eps producing strain. this study investigated structure-function relationships of eps in baking application. the dextransucrase dsrm and the reuteransucrase gtfa were cloned from weissella cibaria 10m and lactobacillus reuteri tmw1.656, respectively, and heterologously expressed in escherichia coli. site-dir ... | 2016 | 27321351 |
| molecular cloning of kman coding for mannanase from klebsiella oxytoca kub-cw2-3 and its hybrid mannanase characters. | gene encoding for β-mannanase (e.c 3.2.1.78) from klebsiella oxytoca kub-cw2-3 was cloned and expressed by an e. coli system resulting in 400 times higher mannanase activities than the wild type. a 3314bp dna fragment obtained revealed an open reading frame of 1164bp, namely kman-2, which encoded for 387 amino acids with an estimated molecular weight of 43.2kda. it belonged to the glycosyl hydrolase family 26 (gh26) exhibited low similarity of 50-71% to β-mannanase produced by other microbial so ... | 2016 | 27233126 |