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reduction of ochratoxin a in chicken feed using probiotic.mycotoxins present in fodders may evoke health problems of animals and people. the data published by fao in 2001 show that 25% of raw materials are contaminated with mycotoxins, while their type and concentration are to a great extent dependable on the climatic zone. biological detoxification of mycotoxins by the use of microorganisms is one of the well-known strategies for the management of mycotoxins in foods and feeds. the aim of this study was to determine the influence of spontaneous fermen ...201425528900
prevalence of antibiotic resistance in lactic acid bacteria isolated from the faeces of broiler chicken in malaysia.probiotics are commonly used as feed additive to substitute antibiotic as growth promoter in animal farming. probiotic consists of lactic acid bacteria (lab), which enhance the growth and health of the animal. probiotic also have higher possibility to become pathogenic bacteria that may carry antibiotic resistant gene that can be transmitted to other lab species. the aim of this study was to identify the lab species in the faeces of broiler chicken and to determine the prevalence of antibiotic r ...201424447766
a review on traditional turkish fermented non-alcoholic beverages: microbiota, fermentation process and quality characteristics.shalgam juice, hardaliye, boza, ayran (yoghurt drink) and kefir are the most known traditional turkish fermented non-alcoholic beverages. the first three are obtained from vegetables, fruits and cereals, and the last two ones are made of milk. shalgam juice, hardaliye and ayran are produced by lactic acid fermentation. their microbiota is mainly composed of lactic acid bacteria (lab). lactobacillus plantarum, lactobacillus brevis and lactobacillus paracasei subsp. paracasei in shalgam fermentati ...201323859403
dynamics and rrna transcriptional activity of lactococci and lactobacilli during cheddar cheese ripening.cheddar cheese is a complex ecosystem where both the bacterial population and the cheese making process contribute to flavor and texture development. the aim of this study was to use molecular methods to evaluate the impact of milk heat treatment and ripening temperature on starter lactococci and non-starter lactic acid bacteria (nslab) throughout ripening of cheddar cheese. eight cheddar cheese batches were manufactured (four with thermized and four with pasteurized milk) and ripened at 4, 7 an ...201323850855
a description of the lactic acid bacteria microbiota associated with the production of traditional fermented vegetables in vietnam.an important part of the daily nourishment in vietnam constitutes of fermented vegetables. bacteria and especially lactic acid bacteria play a central role in the production of many fermented vegetables. the current study was conducted to investigate the diversity of native lactic acid bacteria (lab) populations in 'dua muoi' (mustard and beet fermentation) and 'ca muoi' (eggplant fermentation), three types of popular traditional fermented vegetables of vietnamese origin. to this end a polyphasi ...201323500611
effects of intraduodenal injection of lactobacillus brevis sbc8803 on autonomic neurotransmission and appetite in rodents.lactobacilli provide several health benefits to mammals, including humans. we previously observed that in rats, intraduodenal injection of lactobacillus johnsonii la1 elevated efferent gastric vagal nerve activity (efferent-gvna), while lactobacillus paracasei st11 suppressed efferent-gvna, and thereby increased or decreased food intake. to determine the function of lactobacillus brevis (sbc8803), its effect on food intake was examined by providing food containing heat-killed sbc8803 to mice. we ...201323391754
protective effect of lactofermented red beetroot juice against aberrant crypt foci formation, genotoxicity of fecal water and oxidative stress induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine in rats model.the aim of the study was to investigate the effects of beetroot juice fermented by lactobacillus brevis 0944 and lactobacillus paracasei 0920 (fbj) on carcinogen induction of aberrant crypt foci (acf) in rat colon. 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (phip) was used as carcinogen, which was administrated intragastrically at a dose of 10 μg/day, every day of the experiment. additionally, we investigated the cytotoxicity and genotoxicity of fecal water from experimental animals in the ...201222995401
a novel rhamnose-rich hetero-exopolysaccharide isolated from lactobacillus paracasei dg activates thp-1 human monocytic cells.lactobacillus paracasei dg is a bacterial strain with recognized probiotic properties and is used in commercial probiotic products. however, the mechanisms underlying its probiotic properties are mainly unknown. in this study, we tested the hypothesis that the ability of strain dg to interact with the host is at least partly associated with its ability to synthesize a surface-associated exopolysaccharide (eps). comparative genomics revealed the presence of putative eps gene clusters in the dg ge ...201727913418
target identification of volatile metabolites to allow the differentiation of lactic acid bacteria by gas chromatography-ion mobility spectrometry.the purpose of this work was to study the potential of gas chromatography-ion mobility spectrometry (gc-ims) to differentiate lactic acid bacteria (lab) through target identification and fingerprints of volatile metabolites. the lab selected were used as reference strains for their influence in the flavour of cheese. the four strains of lab can be distinguished by the fingerprints generated by the volatile organic compounds (vocs) emitted. 2-butanone, 2-pentanone, 2-heptanone and 3-methyl-1-buta ...201727855912
antidiabetic (type 2) effects of lactobacillus g15 and q14 in rats through regulation of intestinal permeability and microbiota.the antidiabetic effects of lactobacillus. paracasei subsp. paracasei g15 and lactobacillus. casei q14 in high fat diet and streptozotocin-induced type 2 diabetic (t2d) rats were evaluated in this study. the strains were separated from chinese traditional fermented dairy food. administration of g15 and q14 for 6 weeks significantly improved the glucose tolerance and reduced the hba1c levels in t2d rats. the probiotic treatment reduced the intestinal mucosal permeability and improved the epitheli ...201627713957
the spxb gene as a target to identify lactobacillus casei group species in cheese.this study focused on the spxb gene, which encodes for pyruvate oxidase. the presence of spxb in the genome and its transcription could be a way to produce energy and allow bacterial growth during carbohydrate starvation. in addition, the activity of pyruvate oxidase, which produces hydrogen peroxide, could be a mechanism for interspecies competition. because this gene seems to provide advantages for the encoding species for adaptation in complex ecosystems, we studied spxb in a large set of che ...201627375244
improved eradication rate of standard triple therapy by adding bismuth and probiotic supplement for helicobacter pylori treatment in thailand.helicobacter pylori (h. pylori) remains an important cause of gastric cancer and peptic ulcer disease worldwide. treatment of h. pylori infection is one of the effective ways to prevent gastric cancer. however, standard triple therapy for h. pylori eradication is no longer effective in many countries, including thailand. this study was designed to evaluate the efficacy of adding bismuth and probiotic to standard triple therapy for h. pylori eradication.201425520127
adhesions of extracellular surface-layer associated proteins in lactobacillus m5-l and q8-l.surface-layer associated proteins (slap) that envelop lactobacillus paracasei ssp. paracasei m5-l and lactobacillus casei q8-l cell surfaces are involved in the adherence of these strain to the human intestinal cell line ht-29. to further elucidate some of the properties of these proteins, we assessed the yields and expressions of slap under different incubation conditions. an efficient and selective extraction of slap was obtained when cells of lactobacillus were treated with 5 m licl at 37°c i ...201626709174
labeling quality and molecular characterization studies of products containing lactobacillus spp. strains.the objective of the study was to characterize at species level by phenotypic and different molecular methods the strains of lactobacillus spp. used as constituents of five oral and four vaginal products. susceptibilities to representative antibiotics were evaluated. in addition, total viable counts at mid and 3 months to deadline of shelf life, in the different formulations and the presence of eventual contaminant microorganisms were investigated.in all oral products the molecular characterizat ...201626667227
genomic and functional characterization of the unusual plock 0919 plasmid harboring the spacba pili cluster in lactobacillus casei lock 0919.here, we report the extensive bioinformatic and functional analyses of the unusual plock 0919, a plasmid originating from the probiotic lactobacillus casei lock 0919 strain. this plasmid is atypical because it harbors the spacba-srtc gene cluster encoding spacba pili. we show that all other spacba-srtc sequences of the lactobacillus genus that have been previously described and deposited in genbank are present in the chromosomal dna. another important observation for plock 0919 is that the spacb ...201526637469
effect of milk fermentation by kefir grains and selected single strains of lactic acid bacteria on the survival of mycobacterium bovis bcg.mycobacterium bovis that causes bovine tuberculosis (btb) can be transmitted to humans thought consumption of raw and raw fermented milk products from diseased animals. lactic acid bacteria (lab) used in popular traditional milk products in africa produce anti-microbial compounds that inhibit some pathogenic and spoilage bacteria. m. bovis bcg is an attenuated non-pathogenic vaccine strain of m. bovis and the aim of the study was to determine the effect of the fermentation process on the surviva ...201626544204
genomic diversity of phages infecting probiotic strains of lactobacillus paracasei.strains of the lactobacillus casei group have been extensively studied because some are used as probiotics in foods. conversely, their phages have received much less attention. we analyzed the complete genome sequences of five l. paracasei temperate phages: cl1, cl2, ilp84, ilp1308, and ia2. only phage ia2 could not replicate in an indicator strain. the genome lengths ranged from 34,155 bp (ia2) to 39,474 bp (cl1). phages ia2 and ilp1308 (34,176 bp) possess the smallest genomes reported, thus fa ...201526475105
clinical characteristics of bacteraemia caused by lactobacillus spp. and antimicrobial susceptibilities of the isolates at a medical centre in taiwan, 2000-2014.the clinical characteristics of 89 patients with lactobacillus bacteraemia treated at a university-affiliated hospital in northern taiwan during 2000-2014 were retrospectively evaluated. lactobacillus spp. were identified by 16s rrna sequencing analysis and matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof/ms). antimicrobial susceptibilities of the isolates were determined by broth microdilution. the most commonly isolated species was lactobacillus salivariu ...201526298673
the dnaj gene as a molecular discriminator to differentiate among species and strain within the lactobacillus casei group.identifying lactobacillus casei and its closely related taxa at the species and strain level using only phenotypic and genotypic (16s rdna sequence homology analysis) techniques often yields inaccurate results. in this study, the dnaj chaperone gene was investigated as a molecular target for inter- and intraspecies discrimination within the lb. casei group as well as for the development of specific primers for species identification. the results showed that most of the examined strains could be ...201526050941
an assessment of adhesion, aggregation and surface charges of lactobacillus strains derived from the human oral cavity.there is limited information concerning the adhesion and aggregation of human oral lactobacilli. in this study, the adhesion of 10 lactobacillus species was investigated using h357 oral keratinocyte cells as an in vitro model for oral mucosa. coaggregation with the representative oral pathogen, streptococcus mutans atcc 25175, and the physicochemical cell properties was also evaluated. the results demonstrated significant variations in adhesion (42-96%) and aggregation (autoaggregation, 14-95%; ...201525913304
in vitro modulation of tumor necrosis factor α production in thp-1 cells by lactic acid bacteria isolated from healthy human infants.the human microbiota is a source of probiotics capable of modulating the host immune system. in this study, we collected fecal samples from 100 healthy infants and isolated lactic acid bacteria which were screened for immune modulating effects on tumor necrosis factor α (tnf-α) production. cell-free culture supernatants from 26 isolates were able to decrease tnf-α production in vitro and three of the isolates were selected as candidate probiotics (msmc39-1, msmc39-3, msmc57-1). these isolates we ...201525759008
role of probiotics and functional foods in health: gut immune stimulation by two probiotic strains and a potential probiotic yoghurt.there are numerous reports that show the benefits on the health attributed to the probiotic consumptions. most of the studies were performed using animal models and only some of them were validated in controlled human trials. the present review is divided in two sections. in the first section we describe how the probiotic microorganisms can interact with the intestinal epithelial cells that are the first line of cell in the mucosal site, focusing in the studies of two probiotic strains: lactobac ...201525516152
high resolution melting analysis (hrm) as a new tool for the identification of species belonging to the lactobacillus casei group and comparison with species-specific pcrs and multiplex pcr.the correct identification and characterisation of bacteria is essential for several reasons: the classification of lactic acid bacteria (lab) has changed significantly over the years, and it is important to distinguish and define them correctly, according to the current nomenclature, avoiding problems in the interpretation of literature, as well as mislabelling when probiotic are used in food products. in this study, species-specific pcr and hrm (high-resolution melting) analysis were developed ...201525475306
effects of elaidic acid, a predominant industrial trans fatty acid, on bacterial growth and cell surface hydrophobicity of lactobacilli.the consumption of trans fatty acids (tfas) increases the risk of cardiovascular diseases and coronary heart disease in human, and there are no effective ways to remove tfas after consumption. the aim of this study was to investigate the effects of elaidic acid on bacterial growth, cell surface hydrophobicity of lactobacilli, and metabolism of elaidic acid by lactobacilli. lactobacilli were inoculated in mrs broth containing 0, 100, 200, and 500 mg/l of elaidic acid. viable cell counts of lactob ...201425384717
microbial ecology dynamics reveal a succession in the core microbiota involved in the ripening of pasta filata caciocavallo pugliese cheese.pyrosequencing of the 16s rrna targeting rna, community-level physiological profiles made with biolog ecoplates, proteolysis, and volatile component (voc) analyses were mainly used to characterize the manufacture and ripening of the pasta filata cheese caciocavallo pugliese. plate counts revealed that cheese manufacture affected the microbial ecology. the results agreed with those from culture-independent approaches. as shown by urea-page, reverse-phase high pressure liquid chromatography (rp-hp ...201425085486
the effect of five probiotic lactobacilli strains on the growth and biofilm formation of streptococcus mutans.to compare the effects of five probiotic lactobacilli strains on the growth and biofilm formation of streptococcus mutans (ms).201524806217
complete genome sequence of the probiotic strain lactobacillus casei (formerly lactobacillus paracasei) lock919.lactobacillus casei is usually regarded as a bacterium that lives naturally in the human intestinal tract, where it can contribute to host health and well-being. we describe here the complete genome sequence of l. casei lock919, a strain with probiotic properties isolated from child feces. the genome consists of a 3.11-mb chromosome and a 29,768-bp plasmid.201324072862
diversity and dynamic of lactic acid bacteria strains during aging of a long ripened hard cheese produced from raw milk and undefined natural starter.the aim of this study was to explore diversity and dynamic of indigenous lab strains associated with a long ripened hard cheese produced from raw milk and undefined natural starter such as pdo grana padano cheese. samples of milk, curd, natural whey culture and cheeses (2nd, 6th, 9th and 13th months of ripening) were collected from 6 cheese factories in northern italy. dna was extracted from each sample and from 194 lab isolates. trna(ala)-23s rdna-rflp was applied to identify isolates. strain d ...201324010599
impact of lactobacilli on orally acquired listeriosis.listeria monocytogenes is a foodborne pathogen that crosses the intestinal barrier and disseminates within the host. here, we report a unique comprehensive analysis of the impact of two lactobacillus species, lactobacillus paracasei cncm i-3689 and lactobacillus casei bl23, on l. monocytogenes and orally acquired listeriosis in a gnotobiotic humanized mouse model. we first assessed the effect of treatment with each lactobacillus on l. monocytogenes counts in host tissues and showed that each dec ...201223012479
fermentation of sugars and sugar alcohols by plaque lactobacillus strains.the objective was to analyse the ability of lactobacillus strains isolated from supragingival plaque of subjects with hyposalivation and from healthy controls to ferment sugars and sugar alcohols.201322956128
accelerated ripening of caciocavallo pugliese cheese with attenuated adjuncts of selected nonstarter lactobacilli.the nonstarter lactic acid bacteria lactobacillus plantarum cc3m8, lactobacillus paracasei cc3m35, and lactobacillus casei lc01, previously isolated from aged caciocavallo pugliese cheese or used in cheesemaking, were used as adjunct cultures (ac) or attenuated (by sonication treatment) adjunct cultures (aac) for the manufacture of caciocavallo pugliese cheese on an industrial scale. preliminary studies on the kinetics of growth and acidification and activities of several enzymes of aac were cha ...201222916882
inventory of non starter lactic acid bacteria from ripened parmigiano reggiano cheese as assessed by a culture dependent multiphasic approach.the objective of this study was to investigate microbial species diversity and strain complexity of the cultivable non starter lactic acid bacteria (nslab) occurring in 31 ripened parmigiano reggiano (pr) cheeses. dereplication of 127 lactobacilli isolates by (gtg)(5)-pcr fingerprinting yielded a total of 51 genotypes. phylogenetic relatedness of all the genotypes with known lactobacillus species was determined by a novel combined amplified 16s rdna restriction analysis (16s-ardra), species-spec ...201222626626
lactocepin secreted by lactobacillus exerts anti-inflammatory effects by selectively degrading proinflammatory chemokines.the intestinal microbiota has been linked to inflammatory bowel diseases (ibd), and oral treatment with specific bacteria can ameliorate ibd. one bacterial mixture, vsl#3, containing lactobacillus, bifidobacterium, and streptococcus, was clinically shown to reduce inflammation in ibd patients and normalize intestinal levels of ip-10, a lymphocyte-recruiting chemokine, in a murine colitis model. we identified lactobacillus paracasei prtp-encoded lactocepin as a protease that selectively degrades ...201222520466
manufacture of fior di latte cheese by incorporation of probiotic lactobacilli.this work aimed to select heat-resistant probiotic lactobacilli to be added to fior di latte (high-moisture cow milk mozzarella) cheese. first, 18 probiotic strains belonging to lactobacillus casei, lactobacillus delbrueckii ssp. bulgaricus, lactobacillus paracasei, lactobacillus plantarum, lactobacillus rhamnosus, and lactobacillus reuteri were screened. resistance to heating (65 or 55°c for 10 min) varied markedly between strains. adaptation at 42°c for 10 min increased the heat resistance at ...201222281315
improvement of ayran quality by the selection of autochthonous microbial cultures.ayran is a traditional turkish milk drink which is fermented and salted. inadequate production and storage conditions contribute to its variable organoleptic quality and stability during shelf-life. a thorough physico-chemical, nutritional and microbial characterization of artisanal ayran was carried out in order to standardize its overall quality without altering its original traits. ayran microbial ecosystem was largely dominated by streptococcus thermophilus (st) and lactobacillus delbrueckii ...201627554150
novel method based on chromogenic media for discrimination and selective enumeration of lactic acid bacteria in fermented milk products.microbial analyses of fermented milk products require selective methods to discriminate between close species simultaneously present in high amounts. a culture-based method combining novel chromogenic agar media and appropriate incubation conditions was developed to enumerate lactic acid bacteria (lab) strains in fermented milk. m1 agar, containing two chromogenic substrates, allowed selective enumeration of lactobacillus rhamnosus, two strains of lactobacillus paracasei subsp. paracasei and str ...201626742619
comparison of the growth of lactobacillus delbrueckii, l. paracasei and l. plantarum on inulin in co-culture systems.lactobacillus delbrueckii tu-1, which apparently takes intact inulin into its cells and then degrades it intracellularly, was co-cultured in vitro with l. paracasei ktn-5, an extracellular inulin degrader; or l. plantarum 22a-3, a strain that is able to utilize fructose but not inulin; or both in order to prequalify inulin as a prebiotic agent in vivo. when l. delbrueckii tu-1 was co-cultured with l. paracasei ktn-5 on fructose or inulin, the growth of l. delbrueckii tu-1 on inulin was markedly ...201425379361
improvement of fermented chinese cabbage characteristics by selected starter cultures.traditional fermented vegetables have inconsistent quality and high nitrite content, whereas a commercial starter culture could overcome these problems. a total of 34 lactic acid bacteria strains were screened from 2 homemade naturally fermented chinese cabbages. fermented characters of single starter or mixed cultures were examined, including acidification, growth rate, and nitrite depletion ability in the fermented culture. as a result, the combined starter culture (lactobacillus delbrueckii i ...201424974856
the microfloras and sensory profiles of selected protected designation of origin italian cheeses.approximately 39 italian cheeses carry protected designation of origin (pdo) status. these cheeses differ in their manufacturing technology and the microbial flora which comprise the finished products. the evolution of lactic microflora in cheeses with pdo status is of particular interest because the biochemical activities of these organisms participate in cheesemaking and may play an acknowledged role in the development of organoleptic characteristics during ripening. nonstarter lactic acid bac ...201426082116
difference in degradation patterns on inulin-type fructans among strains of lactobacillus delbrueckii and lactobacillus paracasei.lactobacillus delbrueckii strains were assessed for their degradation patterns of various carbohydrates with specific reference to inulin-type fructans in comparison with those of lactobacillus paracasei strains. firstly, growth curves on glucose, fructose, sucrose and inulin-type fructans with increasing degrees of fructose polymerization (i.e., 1-kestose, fructo-oligosaccharides and inulin) of the strains were compared. l. paracasei dsm 20020 grew well on all these sugars, while the growth rat ...201324936375
stability evaluation of freeze-dried lactobacillus paracasei subsp. tolerance and lactobacillus delbrueckii subsp. bulgaricus in oral capsules.freeze-drying is a common preservation technology in the pharmaceutical industry. various studies have investigated the effect of different cryoprotectants on probiotics during freeze-drying. however, information on the effect of cryoprotectants on the stability of some lactobacillus strains during freeze-drying seems scarce. therefore, the aim of the present study was to establish production methods for preparation of oral capsule probiotics containing lactobacillus paracasei subsp. tolerance a ...201223181077
lactobacillus and leuconostoc volatilomes in cheese conditions.new strains are desirable to diversify flavour of fermented dairy products. the objective of this study was to evaluate the potential of leuconostoc spp. and lactobacillus spp. in the production of aroma compounds by metabolic fingerprints of volatiles. eighteen strains, including five lactobacillus species (lactobacillus fermentum, lactobacillus helveticus, lactobacillus paracasei, lactobacillus rhamnosus, lactobacillus sakei) and three leuconostoc species (leuconostoc citreum, leuconostoc lact ...201626685674
assessment and comparison of probiotic potential of four lactobacillus species isolated from feces samples of iranian infants.the probiotic potential of lactobacillus species isolated from infant feces was investigated. for this study, the antibiotic susceptibility, tolerance in gut-related conditions, antimicrobial activity, and ability to adhere to a human colorectal adenocarcinoma cell line (caco-2 cells) of four common lactobacillus species (lactobacillus paracasei [n = 15], lactobacillus rhamnosus [n = 45], lactobacillus gasseri [n = 20] and lactobacillus fermentum [n = 18]) were assessed. most isolates that which ...201626648174
antibacterial activity of lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria.lactobacilli are normal microflora of the gastrointestinal (gi) tract and are a heterogeneous group of lactic acid bacteria (lab). lactobacillus strains with probiotic activity may have health benefits for human. this study investigates the probiotic potential of lactobacillus strains obtained from the feces of healthy infants and also explores antibacterial activity of lactobacillus strains with probiotic potential against enteropathogenic bacteria. fecal samples were collected from 95 healthy ...201525930687
children with atopic dermatitis show clinical improvement after lactobacillus exposure.the role of probiotics in the treatment of atopic dermatitis (ad) is not clearly established. further clinical trials with new probiotic formulations are warranted.201525600169
quantitative high-resolution melting pcr analysis for monitoring of fermentation microbiota in sourdough.current methods of monitoring the microbial ecology of food fermentation system are generally labor intensive and/or time consuming. this study developed two methods based on high-resolution melting curves (hrm) to monitor sourdough microbiota during fermentation and to investigate the effect of cereal substrate on microbial ecology. a strain cocktail of lactobacillus fermentum fua3165, lactobacillus plantarum fua3309, lactobacillus paracasei fua3166 and lactobacillus reuteri fua3168 was used to ...201424984221
effect of adjuncts on microbiological and chemical properties of scamorza cheese.scamorza is a semi-hard, pasta filata cheese resembling low-moisture mozzarella cheese, with a short ripening time (<30d). scamorza has a bland flavor and, to provide diversification from similar cheeses, it was manufactured using 2 types of milk in the current study: 100% italian friesian milk (f) or 90% f and 10% jersey cow milk (mixed, m), and 2 types of starter: streptococcus thermophilus or s. thermophilus with peptidolytic lactococcus lactis, lactobacillus helveticus, and lactobacillus par ...201525582584
effect of adjuncts on sensory properties and consumer liking of scamorza cheese.the present study aimed to evaluate the effect of a peptidolytic adjunct (lactococcus lactis, lactobacillus helveticus, and lactobacillus paracasei), as a tool to accelerate ripening, on sensory properties and acceptability of scamorza cheese obtained using 2 types of milk (friesian and friesian+jersey) and streptococcus thermophilus as primary starter. a 10-member panel was trained using a specific frame of references and used a specific vocabulary to assess cheese sensory properties through qu ...201525465632
comparison of innate immune activation after prolonged feeding of milk fermented with three species of lactobacilli.the present investigation aimed at identifying the abilities of three different species of probiotic lactobacilli to modulate cellular immune responses in mouse neutrophils and macrophages in vivo over a study period of 60 days. neutrophil respiratory burst enzymes (cytochrome c reductase and mpo) showed remarkable increased activity (p ≤ 0.01) after consumption of milks fermented by different species of probiotics over 30 and 60 days of feeding trials. enzyme activities (β-galactosidase and β-g ...201324033444
electrophysiological analysis of the mechanism of autonomic action by lactobacilli.autonomic nerves, consisting of both sympathetic and parasympathetic nerves, regulate various bodily functions such as blood pressure, body temperature, glucose metabolism, energy metabolism, and digestion. our studies in rats and mice have demonstrated that food, flavor, and music affect physiological phenomena via changes in autonomic neurotransmissions. intestinal injection of lactobacillus johnsonii la1 (ncc533) suppressed sympathetic nerves that innervate the adrenal gland and kidney of ure ...201125045315
exopolysaccharide-producing probiotic lactobacilli reduce serum cholesterol and modify enteric microbiota in apoe-deficient mice.probiotic bacteria have been associated with a reduction in cardiovascular disease risk, a leading cause of death and disability.201425320181
changes in environmental conditions modify infection kinetics of dairy phages.latent period, burst time, and burst size, kinetic parameters of phage infection characteristic of a given phage/host system, have been measured for a wide variety of lactic acid bacteria. however, most studies to date were conducted in optimal growth conditions of host bacteria and did not consider variations due to changes in external factors. in this work, we determined the effect of temperature, ph, and starvation on kinetic parameters of phages infecting lactobacillus paracasei, lactobacill ...201728391509
influence of freezing temperatures prior to freeze-drying on viability of yeasts and lactic acid bacteria isolated from wine.determine the effect of three different freezing temperatures on post-freeze-drying survival rates of wine yeasts and lactic acid bacteria (lab). to know if a similar freeze-drying protocol can be used for both microorganisms.201728375570
lactobacillus paracasei metabolism of rice bran reveals metabolome associated with salmonella typhimurium growth reduction.this study aimed to determine the effect of a cell-free supernatant of lactobacillus paracasei atcc27092 with and without rice bran extract (rbe) on salmonella typhimurium 14028s growth, and to identify a metabolite profile with antimicrobial functions.201728371001
fermented milk containing lactobacillus paracasei subsp. paracasei cncm i-1518 reduces bacterial translocation in rats treated with carbon tetrachloride.probiotics can prevent pathological bacterial translocation by modulating intestinal microbiota and improving the gut barrier. the aim was to evaluate the effect of a fermented milk containing lactobacillus paracasei subsp. paracasei cncm i-1518 on bacterial translocation in rats with carbon tetrachloride (ccl4)-induced cirrhosis. sprague-dawley rats treated with ccl4 were randomized into a probiotic group that received fermented milk containing lactobacillus paracasei subsp. paracasei cncm i-15 ...201728368023
high lactic acid and fructose production via mn(2+)-mediated conversion of inulin by lactobacillus paracasei.lactobacillus paracasei dsm 23505 is able to produce high amounts of lactic acid (la) by simultaneous saccharification and fermentation (ssf) of inulin. aiming to obtain the highest possible amounts of la and fructose, the present study is devoted to evaluate the impact of bivalent metal ions on the process of inulin conversion. it was shown that mn(2+) strongly increases the activity of the purified key enzyme β-fructosidase. in vivo, batch fermentation kinetics revealed that the high mn(2+) co ...201728337581
boosting the growth of the probiotic strain lactobacillus paracasei ssp. paracasei f19.single so-called booster substances were added to the fermentation medium of the probiotic strain lactobacillus (l.) paracasei ssp. paracasei f19 to enhance its growth. a wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. cfu counts were used to correlate the increase in od590nm with the increase in viable cell number. sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growt ...201728315928
isolation, characterization and evaluation of the probiotic potential of a novel lactobacillus strain isolated from feta-type cheese.in the present study 45 lactic acid bacteria (lab) strains were isolated from feta-type cheese and were screened for probiotic potential in a series of established in vitro tests, including resistance to low ph, resistance to pepsin and pancreatin and tolerance to bile salts. the strain k5, which displayed properties similar to or even better than the reference strain lactobacillus plantarum atcc 14917, was chosen for further analysis. firstly, multiplex pcr analysis indicated that the novel str ...201728253999
the association of a probiotic with a prebiotic (flortec, bracco) to improve the quality/quantity of spermatozoa in infertile patients with idiopathic oligoasthenoteratospermia: a pilot study.the hypothesis that the assumption of a probiotic associated with a prebiotic (flortec, bracco; one sachet contains: lactobacillus paracasei b21060 5 × 10(9) cells + arabinogalctan 1243 mg + oligo-fructosaccharides 700 mg + l-glutamine 500 mg) could improve the quality and quantity of spermatozoa in idiopathic oligoasthenoteratospermia (iaot) patients to a larger extent than a control substance (starch) was tested. forty-one patients with no chromosomal aberrations were randomized into two group ...201728245352
physiological changes of surface membrane in lactobacillus with prebiotics.synbiotics are always considered to be beneficial in healthy manipulation of gut environment; however, the purpose of this research was to investigate the dominance of synbiotic over the individual potential of probiotics and prebiotics. four different types of prebiotics, fructo-oligosaccharides, raffinose, inulin, and cellobiose, were evaluated based on their varying degree of polymerization, combined each with 2 different lactobacilli strains, including lactobacillus paracasei 276 and lactoba ...201728199749
oral administration of lactobacillus paracasei l9 attenuates pm2.5-induced enhancement of airway hyperresponsiveness and allergic airway response in murine model of asthma.this study investigated allergy immunotherapy potential of lactobacillus paracasei l9 to prevent or mitigate the particulate matter 2.5 (pm2.5) enhanced pre-existing asthma in mice. firstly, we used a mouse model of asthma (a 21-day ovalbumin (ova) sensitization and challenge model) followed by pm2.5 exposure twice on the same day of the last challenge. pm2.5 was collected from the urban area of beijing and underwent analysis for metals and polycyclic aromatic hydrocarbon contents. the results s ...201728199353
live and heat-killed lactobacillus spp. interfere with streptococcus mutans and streptococcus oralis during biofilm development on titanium surface.this research investigates the ability of live and heat-killed (hk) lactic acid bacteria (lab) to interfere with streptococcus mutans atcc 25175 and streptococcus oralis atcc 9811 during biofilm formation.201728193570
genome sequence of lactobacillus paracasei strain lc-ikematsu, isolated from a pineapple in okinawa, japan.the draft genome sequence of lactobacillus paracasei strain lc-ikematsu, isolated from a pineapple in okinawa, was determined. the total length of the 87 contigs was 3.08 mb with a g+c content of 46.2% and 2,946 coding sequences. the genome analysis revealed its biosynthetic ability of 11 amino acids.201728153910
a lactic acid bacterium isolated from kimchi ameliorates intestinal inflammation in dss-induced colitis.some species of lactic acid bacteria have been shown to be beneficial in inflammatory bowel disease (ibd). in the present study, a strain of lactic acid bacterium (lactobacillus paracasei ls2) was isolated from the korean food, kimchi, and was shown to inhibit the development of experimental colitis induced by dextran sulfate sodium (dss). to investigate the role of ls2 in ibd, mice were fed dss in drinking water for seven days along with ls2 bacteria which were administered intragastrically to ...201728124779
stabilization of freeze-dried lactobacillus paracasei subsp. paracasei jcm 8130(t) with the addition of disaccharides, polymers, and their mixtures.although freeze-drying is a widely used dehydration technique for the stabilizing of unstable lactic acid bacteria, lactobacillus paracasei subsp. paracasei jcm 8130(t) (l. paracasei) is destabilized after freeze-drying and subsequent storage. in order to improve the stability of freeze-dried l. paracasei, effects of disaccharides (sucrose and trehalose), polymers (maltodextrin; md and bovine serum albumin; bsa), and their mixtures on the survival rate of freeze-dried l. paracasei were investiga ...201728103748
lactobacillus paracasei modulates lps-induced inflammatory cytokine release by monocyte-macrophages via the up-regulation of negative regulators of nf-kappab signaling in a tlr2-dependent manner.the application of the probiotic lactobacillus is suggested in the treatment of some inflammatory diseases of intestines due to its potential ability to attenuate inflammation. however, the mechanism is not completely understood. in pbmcs, lactobacillus paracasei (l. paracasei) down-regulated the lps-induced production of tnf-α and il-6. using a macrophage-like differentiated thp-1 cell line induced by pma, we investigated the effect of l. paracasei on the production of pro-inflammatory cytokine ...201728088611
effects of infant formula supplemented with prebiotics compared with synbiotics on growth up to the age of 12 mo: a randomized controlled trial.growth is an essential outcome measure for evaluating the safety of infant formulas (if). we investigated the effects of consumption of if supplemented with prebiotics (fructooligosaccharides, fos, and galactooligosaccharides, gos) compared with synbiotics (fos/gos and lactobacillus paracasei ssp. paracasei strain f19) on the growth of healthy infants.201728060791
design of starch-formate compound fibers as encapsulation platform for biotherapeutics.effective encapsulation and protection of biotherapeutics using a bio-based carrier, preferably issued from renewable resources, remains a challenge. herein, we demonstrate application of coaxial electrospinning to fabric starch-based core-sheath compound fibers as a bacterial cells' carrier. starch-formate is employed as an encapsulation agent, while the fiber core is made of glycerol, serving as a cell suspension medium. sem microscopy reveals a distinct core-sheath morphology of the starch-fo ...201728024544
prevention of hypertension-induced vascular dementia by lactobacillus paracasei subsp. paracasei ntu 101-fermented products.numerous etiological studies have established positive clinical association between hypertension and vascular dementia (vad). lactobacillus paracasei subsp. paracasei ntu 101-fermented products have been shown to decrease vascular risk factors such as hypertension, atherosclerosis, hyperlipidemia and obesity.201727937042
microencapsulated cells of lactobacillus paracasei subsp. paracasei in biopolymer complex coacervates and their function in a yogurt matrix.l. paracasei subsp. paracasei e6 cells were encapsulated by complex coacervation using whey protein isolate (wpi) and gum arabic and introduced in stirred yogurts after fermentation. for comparison purposes, yogurts without addition of l. paracasei and yogurts with free cells of l. paracasei were produced. the survival of free and microencapsulated l. paracasei cells was evaluated during storage of the yogurts for 45 days at 4 °c. in addition, yogurts were exposed to simulated gastric juice and ...201727935607
the water kefir grain inoculum determines the characteristics of the resulting water kefir fermentation process.to investigate the influence of the water kefir grain inoculum on the characteristics of the water kefir fermentation process.201727930854
volatile compounds produced by lactobacillus paracasei during oat fermentation.this study investigated the profiles of volatile compounds produced by lactobacillus paracasei during oat fermentation using gas chromatography-mass spectrometry coupled with headspace solid-phase microextraction method. a total of 60 compounds, including acids, alcohols, aldehydes, esters, furan derivatives, hydrocarbons, ketones, sulfur-containing compounds, terpenes, and other compounds, were identified in fermented oat. lipid oxidation products such as 2-pentylfuran, 1-octen-3-ol, hexanal, a ...201627925257
daily ingestion of the probiotic lactobacillus paracasei st11 decreases vaccinia virus dissemination and lethality in a mouse model.vaccinia virus (vacv) is an important pathogen. although studies have shown relationships between probiotics and viruses, the effect of probiotics on vacv infection is unknown. therefore, this work aims to investigate the probiotics effects on vacv infection. mice were divided into four groups, two non-infected groups, one receiving the probiotic, the other one not receiving it, and two groups infected intranasally with vacv western reserve (vacv-wr) receiving or not receiving the probiotic. vir ...201727873547
maldi-tof mass spectrometry enables a comprehensive and fast analysis of dynamics and qualities of stress responses of lactobacillus paracasei subsp. paracasei f19.lactic acid bacteria (lab) are widely used as starter cultures in the manufacture of foods. upon preparation, these cultures undergo various stresses resulting in losses of survival and fitness. in order to find conditions for the subsequent identification of proteomic biomarkers and their exploitation for preconditioning of strains, we subjected lactobacillus (lb.) paracasei subsp. paracasei tmw 1.1434 (f19) to different stress qualities (osmotic stress, oxidative stress, temperature stress, ph ...201627783652
lactobacillus paracasei endocarditis in a consumer of probiotics with advanced and severe bicuspid aortic valve stenosis complicated with diffuse left ventricular mid-layer fibrosis. 201627657466
heterologous expression and delivery of biologically active exendin-4 by lactobacillus paracasei l14.exendin-4, a glucagon-like protein-1 (glp-1) receptor agonist, is an excellent therapeutic peptide drug for type 2 diabetes due to longer lasting biological activity compared to glp-1. this study explored the feasibility of using probiotic lactobacillus paracasei as an oral vector for recombinant exendin-4 peptide delivery, an alternative to costly chemical synthesis and inconvenient administration by injection. l. paracasei transformed with a plasmid encoding the exendin-4 gene (l. paracasei l1 ...201627764251
short communication: change of naturally occurring benzoic acid during skim milk fermentation by commercial cheese starters.this study sought to investigate the change of naturally occurring benzoic acid (ba) during skim milk fermentation by 4 kinds of commercial cheese starters used in domestic cheese. the culture was incubated at 3-h intervals for 24h at 30, 35, and 40°c. the ba content during fermentation by streptococcus thermophilus stb-01 was detected after 12h at all temperatures, sharply increasing at 30°c. in lactobacillus paracasei lc431, ba was detected after 9h at all temperatures, sharply increasing unti ...201627592433
effect of lactobacillus paracasei culture filtrates and artichoke polyphenols on cytokine production by dendritic cells.the most recent trend in research on probiotic bacteria aims at the exploitation of bioactive bacterial compounds that are responsible for health-promoting effects and suitable for medical applications. therefore, the main purpose of this study was to ascertain if the immunomodulatory effects of l. paracasei strains on dendritic cells (dcs) were caused by bacterial metabolites released in the culture medium. for that reason, bacterial strains were grown in two media generally used for the cultur ...201627754398
effects of deep sea water and lactobacillus paracasei subsp. paracasei ntu 101 on hypercholesterolemia hamsters gut microbiota.hypercholesterolemia is a common metabolic syndrome in modern human society. despite that the alteration of host gut microbiota has been linked to hypercholesterolemia in previous studies, the key host-microbiota interaction of hypercholesterolemia remains elusive. lactobacillus paracasei subsp. paracasei ntu 101 (ntu 101) and deep sea water (dsw) were known for cholesterol-lowering potential. the impact of ntu 101 and dsw on hamster gut microbiota was investigated side-by-side using denaturing ...201727709286
shortening of the lactobacillus paracasei subsp. paracasei bgnj1-64 agglb protein switches its activity from auto-aggregation to biofilm formation.agglb is the largest (318.6 kda) aggregation-promoting protein of lactobacillus paracasei subsp. paracasei bgnj1-64 responsible for forming large cell aggregates, which causes auto-aggregation, collagen binding and pathogen exclusion in vitro. it contains an n-terminus leader peptide, followed by six successive collagen binding domains, 20 successive repeats (cnab-like domains) and an lpxtg sorting signal at the c-terminus for cell wall anchoring. experimental information about the roles of the ...201627660628
fermented ginseng contains an agonist of peroxisome proliferator activated receptors α and γ.peroxisome proliferator activated receptor (ppar) is a nuclear receptor that is one of the transcription factors regulating lipid and glucose metabolism. fermented ginseng (fg) is a ginseng fermented by lactobacillus paracasei a221 containing minor ginsenosides and metabolites of fermentation. dna microarray analysis of rat liver treated with fg indicated that fg affects on lipid metabolism are mediated by ppar-α. to identify a ppar-α agonist in fg, ppar-α transcription reporter assay-guided fra ...201627627700
individual strains of lactobacillus paracasei differentially inhibit human basophil and mouse mast cell activation.the microbiota controls a variety of biological functions, including immunity, and alterations of the microbiota in early life are associated with a higher risk of developing allergies later in life. several probiotic bacteria, and particularly lactic acid bacteria, were described to reduce both the induction of allergic responses and allergic manifestations. although specific probiotic strains were used in these studies, their protective effects on allergic responses also might be common for al ...201627621812
development of a simple, low-cost and eurytopic medium based on pleurotus eryngii for lactic acid bacteria.lactic acid bacteria (lab) are a group of important beneficial microorganisms for human, but their growth is restricted to the habitats with rich nutrients. in order to develop a simple, low-cost and efficient medium based on the mushroom pleurotus eryngii, this study evaluated the effects of different treatment methods for the mushroom, concentration of the mushroom, buffers, tween 80, mgso4·7h2o, mnso4·4h2o, cuso4·5h2o, riboflavin and ascorbic acid on the growth of lactococcus lactis subsp. la ...201627590888
effect of inhibition on tyrosinase and melanogenesis of agastache rugosa kuntze by lactic acid bacteria fermentation.this work presents the first report that a. rugosa could have tyrosinase and melanogenesis inhibition and that its activities also be improved by fermentation with lactobacillus rhamnosus and lactobacillus paracasei. it was found that the tyrosinase and melanogenesis inhibition was correlated with antioxidant activity of acacetin, the major biologically active substances in a. rugosa.201627531590
do probiotics have a role in the treatment of allergic rhinitis?: a comprehensive systematic review and meta analysis.to investigate clinical evidence for the efficacy of probiotics in the treatment of allergic rhinitis (ar).201627442711
impact of probiotic synbio(®) administered by vaginal suppositories in promoting vaginal health of apparently healthy women.the purpose of this study was to investigate whether vaginal administration of probiotic lactobacillus results in their colonization and persistence in the vagina and whether it promotes normalization and maintenance of ph and nugent score. a single-arm, open-label controlled towards the baseline (pre-post) study including 35 apparently healthy women was conducted. each woman was examined three times during the study. women were instructed to receive daily for 7 days, the probiotic suppositories ...201627324341
immuno-physiological alterations from afb1 in rats counteracted by treatments with lactobacillus paracasei bej01 and montmorillonite clay mixture.high contamination by aflatoxin b1 (afb1) has been detected in beja province (tunisia) in many dairy products and animal feed, which has resulted in many tons of cereals and cereals being removed from the market, causing economic loss. while removal represents a means of reducing risk, exposures still occur. studies have increasingly focused on means of afb1 biodegradation/elimination using lactic acid bacteria and clay mineral. in the study here, lactobacillus paracasei bej01 (lp) and montmoril ...201627294391
selected lactobacillus strains isolated from sugary and milk kefir reduce salmonella infection of epithelial cells in vitro.the isolation of potentially probiotic strains and the subsequent study of their properties are very important steps to gain insight in the health benefits ascribed to sugary and milk kefir. the aim of the present study was to characterise fifteen lactobacillus strains isolated from these beverages by determining some surface properties and their ability to antagonise enterocyte cell damage after salmonella infection in vitro. lactobacillus surface properties were determined by hydrophobicity, a ...201627291404
physio-chemical, microbiological properties of tempoyak and molecular characterisation of lactic acid bacteria isolated from tempoyak.this study aims to determine physio-chemical properties of tempoyak, characterise the various indigenous species of lactic acid bacteria (lab) present at different stages of fermentation and also to determine the survival of selected foodborne pathogens in tempoyak. the predominant microorganisms present in tempoyak were lab (8.88-10.42 log cfu/g). fructobacillus durionis and lactobacillus plantarum were the dominant members of lab. other lab species detected for the first time in tempoyak were ...201627217364
identification of sirtuin and its target as the ribosomal protein s4 in lactobacillus paracasei.sirtuin is a protein with an enzymatic activity of nad(+)-dependent protein deacetylation. it was first identified in yeast and its homologous genes have been widely found in various organisms. in bacteria, sirtuin gene was first described as cobb, encoding a cobalamin processing enzyme; and later its potential involvement in regulating acetylation levels of metabolic enzymes, transcription factors, chemotactic proteins and others have been reported. in order to study its physiological relevance ...201627118078
a lactobacillus mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function.inorganic polyphosphate (polyp) was previously identified as a probiotic-derived substance that enhances intestinal barrier function. polyp-accumulating bacteria are expected to have beneficial effects on the human gastrointestinal tract. in this study, we selected lactobacillus paracasei jcm 1163 as a strain with the potential to accumulate polyp, because among the probiotic bacteria stored in our laboratory, it had the largest amount of polyp. the chain length of polyp accumulated in l. paraca ...201626966939
variations in prebiotic oligosaccharide fermentation by intestinal lactic acid bacteria.prebiotic oligosaccharides confer health benefits on the host by modulating the gut microbiota. intestinal lactic acid bacteria (lab) are potential targets of prebiotics; however, the metabolism of oligosaccharides by lab has not been fully characterized. here, we studied the metabolism of eight oligosaccharides by 19 strains of intestinal lab. among the eight oligosaccharides used, 1-kestose, lactosucrose and galactooligosaccharides (goss) led to the greatest increases in the numbers of the str ...201626888650
erratum to: metabolic fingerprinting of lactobacillus paracasei: the optimal quenching strategy. 201626790754
purification and partial characterization of a novel bacteriocin synthesized by lactobacillus paracasei hd1-7 isolated from chinese sauerkraut juice.bacteriocins have antimicrobial activities against food-spoiling bacteria and food-borne pathogens. paracin 1.7, a bacteriocin synthesized by lactobacillus paracasei hd1-7 isolated from chinese sauerkraut juice, was studied. following partial purification with ammonium sulfate precipitation, cm sepharose fast flow, and sephadex g-10 chromatography, the molecular weight of paracin 1.7 was about 10 kda based on tricine-sds-page results. a 2.87 fold purified bacteriocin was produced, reaching a fin ...201626763314
cow's milk and rice fermented with lactobacillus paracasei cba l74 prevent infectious diseases in children: a randomized controlled trial.fermented foods have been proposed for the prevention of infectious diseases. we evaluated the efficacy of fermented foods in reducing common infectious diseases (cids) in children attending daycare.201726732025
the effects of non-viable lactobacillus on immune function in the elderly: a randomised, double-blind, placebo-controlled study.forty-two participants in two nursing homes who were ≥65 years of age were randomised to receive a jelly containing 10 billion heat-killed lactobacillus paracasei mcc1849 cells (lp group) or a placebo jelly without lactobacilli (placebo group) for 6 weeks. three weeks after beginning jelly intake, all subjects received an influenza vaccination (a/h1n1, a/h2n3 and b). blood samples were collected before and after the treatment period. there were no significant differences in immune parameters, in ...201626708938
oral administration of lactobacillus paracasei ncc 2461 for the modulation of grass pollen allergic rhinitis: a randomized, placebo-controlled study during the pollen season.the efficacy of lactobacillus paracasei ncc 2461 in modulating allergic rhinitis was previously evaluated in two exploratory clinical studies. oral administration with ncc 2461 reduced specific subjective symptoms following nasal provocation tests with controlled grass pollen allergen concentrations. our aim was to confirm the anti-allergic effect of ncc 2461 in grass pollen allergic subjects exposed to natural doses of allergens during the pollen season.201526664720
complete nucleotide sequence of the 16s rrna from lactobacillus paracasei hs-05 isolated from women's hands.we determined the complete nucleotide sequence of the 16s rrna from a new bacterium collected from the surfaces of women's hands. we also compared the presence of various bacteria based on the subjects' sex and age. the number of colonies isolated from the hand surface was larger for women than men, and the largest number of isolates was confirmed to be present for the women in their 30 s and men in their 40 s (147 and 34 isolates, respectively). the morphology of an isolated bacterial strain wa ...201526660786
enhanced l-lactic acid production in lactobacillus paracasei by exogenous proline addition based on comparative metabolite profiling analysis.this study investigated cell physiological and metabolic responses of lactobacillus paracasei to osmotic stresses. both cellular fatty acid composition and metabolite profiling were responded by increasing unsaturated and epoxy-fatty acid proportions, as well as accumulating some specific intracellular metabolites. simultaneously, metabolite profiling was adopted to rationally and systematically discover potential osmoprotectants. consequently, exogenous addition of proline or aspartate was vali ...201626658821
metabolic footprinting for investigation of antifungal properties of lactobacillus paracasei.lactic acid bacteria with antifungal properties are applied for biopreservation of food. in order to further our understanding of their antifungal mechanism, there is an ongoing search for bioactive molecules. with a focus on the metabolites formed, bioassay-guided fractionation and comprehensive screening have identified compounds as antifungal. although these are active, the compounds have been found in concentrations that are too low to account for the observed antifungal effect. it has been ...201626573172
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