| polyphasic study of the spatial distribution of microorganisms in mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations. | the distribution of microorganisms in pozol balls, a fermented maize dough, was investigated by a polyphasic approach in which we used both culture-dependent and culture-independent methods, including microbial enumeration, fermentation product analysis, quantification of microbial taxa with 16s rrna-targeted oligonucleotide probes, determination of microbial fingerprints by denaturing gradient gel electrophoresis (dgge), and 16s ribosomal dna gene sequencing. our results demonstrate that dgge f ... | 1999 | 10584005 |
| involvement of manganese in conversion of phenylalanine to benzaldehyde by lactic acid bacteria. | we examined the involvement of mn(ii) in the conversion of phenylalanine to benzaldehyde in cell extracts of lactic acid bacteria. experiments performed with lactobacillus plantarum demonstrated that mn(ii), present at high levels in this strain, is involved in benzaldehyde formation by catalyzing the conversion of phenylpyruvic acid. experiments performed with various lactic acid bacterial strains belonging to different genera revealed that benzaldehyde formation in a strain was related to a hi ... | 1999 | 10584022 |
| crystal versus solution structures of thiamine diphosphate-dependent enzymes. | the quaternary structures of the thiamine diphosphate-dependent enzymes transketolase (ec 2.2.1.1; from saccharomyces cerevisiae), pyruvate oxidase (ec 1.2.3.3; from lactobacillus plantarum), and pyruvate decarboxylase (ec 4.1.1.1; from zymomonas mobilis and brewers' yeast, the latter in the native and pyruvamide-activated forms) were examined by synchrotron x-ray solution scattering. the experimental scattering data were compared with the curves calculated from the crystallographic models of th ... | 2000 | 10617618 |
| use of green fluorescent protein to tag lactic acid bacterium strains under development as live vaccine vectors. | the lactic acid bacteria (lab) are safe microorganisms which are mainly used for the preparation of fermented foods and for probiotic applications. the potential of lab as live vehicles for the production and delivery of therapeutic molecules such as antigens is also being actively investigated today. however, very little is known about the fate of live lab when administered in vivo and about the interaction of these microorganisms with the nasal or gastrointestinal ecosystem. for future applica ... | 2000 | 10618252 |
| processing of quinoa (chenopodium quinoa, willd): effects on in vitro iron availability and phytate hydrolysis. | the effect of different processing techniques was studied on in vitro iron availability and phytate hydrolysis in high and low saponin content quinoa (chenopodium quinoa, willd) seeds. water slurries of ungerminated and germinated quinoa flour were processed by cooking, soaking, and fermentation using lactobacillus plantarum as starter. iron solubility under physiological conditions (in vitro) was measured and used as an estimation of iron availability. phytate (inositol hexaphosphate/ip6) and i ... | 1999 | 10627836 |
| colonic food: pre- and probiotics. | the colonic mucosa is unable to nourish itself from the blood. instead, its nutritive demand must be met from the lumen, where different nutrients, short-chain fatty acids, amino acids, polyamines, growth factors, vitamins, and antioxidants are produced by a nonpathogenic (commensal, so-called "protective" probiotic) flora. the substrates for the production of nutrients are usually referred to as prebiotics, which consist mainly of ingested fibers and complex proteins (colonic food), but may als ... | 2000 | 10634219 |
| probiotics and immune response. | current evidence supports the concept that oral administration of probiotic lactobacilli may be therapeutic in preventing antibiotic-associated diarrhea in children and in reestablishing normal flora in the gastrointestinal tract. children with human immunodeficiency virus (hiv) infections may have episodes of diarrhea and frequently experience malabsorption associated with possible bacterial overgrowth; together these may interact to produce the growth abnormalities characteristic of this group ... | 2000 | 10634225 |
| degradation of cyanogenic glycosides by lactobacillus plantarum strains from spontaneous cassava fermentation and other microorganisms. | strains of lactobacillus plantarum, leuconostoc mesenteroides, candida tropicalis and penicillium sclerotiorum were screened for 19 enzymatic activities using the commercial kit api zym (bio mérieux). this activity was compared to the ability of degrading the toxic cyanogenic glycosides amygdalin, linamarin, and linseed cyanogens (a mixture of linustatin and neolinustatin). good correlation between the beta-glucosidase activity found in the api zym screening and the ability to degrade the cyanog ... | 1999 | 10634708 |
| identification of lactobacillus species of human origin by a commercial kit, api50chl. | the efficacy of a biochemical kit, api50chl kit, for identification of intestinal and vaginal lactobacilli from humans was evaluated by comparing with the results of dna-dna hybridization assay. the results showed that in total only 52 of the 172 strains (30.2%) tested were identified correctly by the kit at species level. especially all strains of some species, such as lactobacillus gasseri and lactobacillus crispatus, were misidentified as lactobacillus acidophilus by the kit. however, the kit ... | 1999 | 10681709 |
| a gene cluster encoding plantaricin 1.25beta and other bacteriocin-like peptides in lactobacillus plantarum tmw1.25. | plantaricin 1.25beta is a thermostable class two bacteriocin produced by lactobacillus plantarum tmw1.25 isolated from sausage fermentation. it is co-produced with several other bacteriocin-like peptides. using oligonucleotides derived from previously determined peptide sequences, a 3.8 kb dna fragment could be amplified. a neighboring 1.8 kb fragment was amplified using ligation-anchored single-specific-primer pcr. sequencing of the complete 5.6 kb stretch revealed that the structural gene for ... | 2000 | 10684981 |
| characterization and determination of origin of lactic acid bacteria from a sorghum-based fermented weaning food by analysis of soluble proteins and amplified fragment length polymorphism fingerprinting. | the group that includes the lactic acid bacteria is one of the most diverse groups of bacteria known, and these organisms have been characterized extensively by using different techniques. in this study, 180 lactic acid bacterial strains isolated from sorghum powder (44 strains) and from corresponding fermented (93 strains) and cooked fermented (43 strains) porridge samples that were prepared in 15 households were characterized by using biochemical and physiological methods, as well as by analyz ... | 2000 | 10698775 |
| effect of process improvement on the physico-chemical properties of infant weaning food from fermented composite blends of cereal and soybeans. | saccharomyces cerevisiae and lactobacillus plantarum atcc 10776 were used as starters to ferment various composite blends of cereals and legumes produced through malting and toasting of two varieties of maize--zea mays (dmr-lsr white & dmr-esr yellow), sorghum--sorghum bicolor (dawa white & dawa red) and one variety of soybeans (glycine ax). compared to the untoasted and unmalted fermented blends, a relatively lower ph (3.6), highly sour product was obtained with 12 h of fermentation. results al ... | 1999 | 10716405 |
| the genetic switch for the regulatory pathway of lactobacillus plantarum phage (phi)g1e: characterization of the promoter p(l), the repressor gene cpg, and the cpg-encoded protein cpg in escherichia coli. | the structural and functional features of the approximately 530 bp p(l)/gb5-gb6-cpg-gb7 region (p(l) overlaps gb5) for the lysogenic pathway of l. plantarum phage (phi)gle were investigated using the cat gene of e. coli plasmid pkk232-8 as a reporter. in e. coli xl1-blue, a recombinant plasmid pkpl2 (cat under p(l)/gb5-gb6) exhibited distinct cat activity, whereas the activity of pkplcp1 (cat under p(l)/gb5-gb6-cpg) was only marginal. when pkpl2 was coexistent with a compatible derivative of pla ... | 2000 | 10721708 |
| [the role of the carbohydrate composition of the glycocalyx in some species of lactobacilli in the manifestation of their adhesive properties]. | availability of certain monosaccharides in the composition of glycocalyx of lactic acid bacteria (lactobacillus plantarum--strains 337d and 11/16; streptococcus thermophilus--strains s1 (nonmucous race) and s5 (mucous race), enterococcus faecium (k-50) has been investigated with the help of plant lectins with certain carbohydrate specificity labelled by colloid gold. all the microorganisms under investigation were characterized by the presence of n-acetyl-d-galactosamine and n-acetyl-d-glucosami ... | 1999 | 10733280 |
| bacteriocin production by lactic acid bacteria isolated from rioja red wines. | forty-two lactic acid bacteria (lab) of the genera lactobacillus (32), leuconostoc (6), pediococcus (3) and lactococcus (1), isolated from rioja red wines, were tested for antimicrobial activity. all these strains, as well as 18 leuconostoc oenos and 19 yeast strains were used as indicators. only nine strains showed antimicrobial activity, and all were of the species lactobacillus plantarum, which constitutes the predominant microflora in rioja red wines after alcoholic fermentation. lact. plant ... | 2000 | 10735242 |
| arabinose fermentation by lactobacillus plantarum in sourdough with added pentosans and alphaalpha-l-arabinofuranosidase: a tool to increase the production of acetic acid. | sixty-five strains of obligately and facultatively heterofermentative sourdough lactic acid bacteria were screened for their capacity to grow optimally in the presence of arabinose, ribose and xylose as carbon sources. lactobacillus alimentarius 15f, lact. brevis 10a, lact. fermentum 1f and lact. plantarum 20b showed higher growth rate, cell yield, acidification rate and production of acetic acid when some pentoses instead of maltose were added to the sdb medium. lactobacillus plantarum 20b used ... | 2000 | 10736001 |
| selective control of lactobacilli in kimchi with nisin. | the use of nisin to control the lactobacilli responsible for the over-ripening of kimchi, traditional korean fermented vegetables, was studied. of the 40 strains of lactic acid bacteria studied, most were sensitive to nisin at a concentration of 100 iu ml-1, while two strains appeared to be resistant. in mrs broth containing nisin at concentrations of 100-300 iu ml-1, the growth of sensitive strains of lactobacillus plantarum was delayed for 2-3 d at 20 degrees c. when nisin was added to kimchi ... | 2000 | 10747245 |
| the effect of lactobacillus buchneri, lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. | several microorganisms and one chemical preservative were tested for their effects on the fermentation and aerobic stability of corn silage. whole-plant corn (one-half milk line, 31.3% dry matter) was ensiled in quadruplicate 20-l laboratory silos untreated or after the following treatments: lactobacillus buchneri at 1 x 10(5) and 1 x 10(6) cfu/g of fresh forage; two different strains of l. plantarum, each at 1 x 10(6) cfu/g; and a buffered propionic acid-based product at 0.1% of fresh forage we ... | 2000 | 10750111 |
| identification and nucleotide sequence of genes involved in the synthesis of lactocin 705, a two-peptide bacteriocin from lactobacillus casei crl 705. | the structural gene determinants of lactocin 705, a bacteriocin produced by lactobacillus casei crl 705, have been amplified from a plasmid of approximately 35 kb and sequenced. lactocin 705 is a class iib bacteriocin, whose activity depends upon the complementation of two peptides (705alpha and 705beta) of 33 amino acid residues each. these peptides are synthesized as precursors with signal sequences of the double-glycine type, which exhibited high identities with the leader peptides of plantar ... | 2000 | 10754241 |
| manganese homeostasis in bacillus subtilis is regulated by mntr, a bifunctional regulator related to the diphtheria toxin repressor family of proteins. | the bacillus subtilis yqhn gene encodes a metalloregulatory protein distantly related to the corynebacterium diphtheriae diphtheria toxin repressor (dtxr). while dtxr mediates the iron-dependent repression of iron uptake, we demonstrate that yqhn (herein renamed mntr) encodes a manganese modulated regulator of manganese transport. an mntr mutant strain is sensitive to both manganese and cadmium, suggesting that the transport of these metals is derepressed. we selected tn10 insertions that suppre ... | 2000 | 10760146 |
| citrate utilization by homo- and heterofermentative lactobacilli. | citrate utilization by several homo- and heterofermentative lactobacilli was determined in kempler and mckay and in calcium citrate media. the last medium with glucose permitted best to distinguish citrate-fermenting lactobacilli. lactobacillus rhamnosus atcc 11443, lactobacillus zeae atcc 15820 and lactobacillus plantarum atcc 8014 used citrate as sole energy source, whereas in the other strains, glucose and citrate were cometabolized. some lactobacilli strains produced aroma compounds from cit ... | 2000 | 10772153 |
| expression of bacillus subtilis phytase in lactobacillus plantarum 755. | phytase enzymes can increase the nutritional value of food and feed by liberating inorganic phosphate from phytate, the major storage form of phosphorus in plants. the phytase (phyc) from bacillus subtilis vtt e-68013 was expressed in lactobacillus plantarum strain 755 using lact. amylovorus alpha-amylase secretion signals. in an overnight cultivation in mrs medium containing cellobiose for induction of the alpha-amylase promoter, catalytically active phytase was secreted as a predominant extrac ... | 2000 | 10792656 |
| [a probiotic as an antagonist of bacterial translocation in experimental pancreatitis]. | infection is the most common cause of death in acute pancreatitis. earlier studies have demonstrated that early enteral nutrition decreases microbial translocation, upregulates the immune function and reduces septic complications and mortality. lactobacillus plantarum (lp) has been shown to be effective in reducing egress of endotoxin and microbial strain that showed very high adherence power to gut mucosa. we adopted a model of acute pancreatitis induced by isolation and ligation of biliopancre ... | 1999 | 10793768 |
| a physical and functional analysis of the newly-identified bglgpt operon of lactobacillus plantarum. | a newly-identified bglgpt operon of lactobacillus plantarum was isolated and expressed in escherichia coli. the sequence analysis of the cloned dna fragment showed three open reading frames encoding (i) a 237-amino acid protein (bglg), (ii) a 577-amino acid protein (bglp) and (iii) a 486-amino acid protein (bglt). bglg, bglp and bglt were shown to be homologous to the bglg family of transcriptional antiterminators, to permeases of the phosphoenolpyruvate-dependent phosphotransferase system and t ... | 2000 | 10802183 |
| alteration of intestinal microflora is associated with reduction in abdominal bloating and pain in patients with irritable bowel syndrome. | the influence of the gastrointestinal (gi) microflora in patients with irritable bowel syndrome (ibs) has not been clearly elucidated. this study was undertaken to see if patients with ibs have an imbalance in their normal colonic flora, as some bacterial taxa are more prone to gas production than others. we also wanted to study whether the flora could be altered by exogenous supplementation. in a previous study we have characterized the mucosa-associated lactobacilli in healthy individuals and ... | 2000 | 10811333 |
| characterization of a phage resistance plasmid, plks, of silage-making lactobacillus plantarum ngri0101. | phage contamination has resulted in abnormal fermentation in silage. we isolated a phage-resistant strain, lactobacillus plantarum ngri0101 from silage. the strain carried two plasmids, plkl (6.8 kb) and plks (2.0 kb). by curing and retransformation of the plasmids, we clarified that plks has phage resistant activity, characterized as no adsorption inhibition. plks has 2,025 bp and three orfs, orfl23, orf132, and orf918. the predicted amino acid sequence of the orf918 product showed high similar ... | 2000 | 10830488 |
| purification and dna-binding properties of the cro-type regulatory repressor protein cng encoded by the lactobacillus plantarum phage phi g1e. | the putative repressor protein cng (10kda on an sds gel) for the lytic pathway of lactobacillus plantarum phage φg1e was purified using the escherichia coli pt7 system, and its dna-binding ability for the seven operator-like sequences, the gatac-boxes (gb1 to gb7), was investigated in vitro. in gel-shift assays, cng selectively bound to the dna fragments containing the gatac-box(es). in addition, dnase i footprinting analysis with supercoiled dna demonstrated that cng can specifically cover ... | 2000 | 10831850 |
| pharmacokinetics of lactobacillus plantarum ncimb 8826, lactobacillus fermentum kld, and lactococcus lactis mg 1363 in the human gastrointestinal tract. | genetically modified lactic acid bacteria may be a way to deliver vaccinal epitopes in the gastrointestinal tract. | 2000 | 10848668 |
| heterogeneity of lactobacillus plantarum isolates from feta cheese throughout ripening. | thirty-two lactobacillus plantarum strains isolated from feta cheese throughout ripening were studied for their phenotypic characteristics, protein profile of cell-free extracts, enzyme profiles, plasmid profiles, proteolytic and acidifying abilities and ability to grow at low ph and in the presence of bile. results showed that some biotechnologically important characteristics, such as acidifying and proteolytic activities, can differ between strains. in addition, different plasmid profiles sugg ... | 2000 | 10849182 |
| stimulatory effect of honey on multiplication of lactic acid bacteria under in vitro and in vivo conditions. | the effect of honey and sucrose on lactic acid bacteria in vitro and in rat gut was studied to determine whether these organisms were affected differently by honey compared with sucrose. under in vitro conditions, the number of lactobacillus acidophilus and lactobacillus plantarum counts increased 10-100 fold in the presence of honey compared with sucrose. feeding of honey to rats also resulted in significant increase in counts of lactic acid bacteria. although there was no significant differenc ... | 2000 | 10849275 |
| lactobacillus plantarum amylase acting on crude starch granules. native isoforms and activity changes after limited proteolysis. | the microheterogeneous native amylolytic complex secreted by the isolate a6 of lactobacillus plantarum revealed a selective enzyme specificity loss when submitted to a limited proteolysis under a suboptimum ph condition. a clear electrophoretic profile change toward just one shorter, more acidic, and equally active polypeptide fragment resulted from the pronase e pretreatment. although the whole enzyme activity remained apparently unaffected for soluble starch, the native parallel activity on in ... | 2000 | 10849830 |
| in lactobacillus plantarum, carbamoyl phosphate is synthesized by two carbamoyl-phosphate synthetases (cps): carbon dioxide differentiates the arginine-repressed from the pyrimidine-regulated cps. | carbamoyl phosphate (cp) is an intermediate in pyrimidine and arginine biosynthesis. carbamoyl-phosphate synthetase (cps) contains a small amidotransferase subunit (gln) that hydrolyzes glutamine and transfers ammonia to the large synthetase subunit (syn), where cp biosynthesis occurs in the presence of atp and co(2). lactobacillus plantarum, a lactic acid bacterium, harbors a pyrimidine-inhibited cps (cps-p; elagöz et al., gene 182:37-43, 1996) and an arginine-repressed cps (cps-a). sequencing ... | 2000 | 10852872 |
| rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna. | rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ... | 2000 | 10856652 |
| the use of a starter culture in the fermentation of cassava for the production of "kivunde", a traditional tanzanian food product. | three cassava fermentation methods (spontaneous fermentation, back-slopping and the use of starter culture) for the production of kivunde, executed in three trials at 30 degrees c, were compared in terms of cyanide level reduction, microbiology and product quality improvement. among the isolates from spontaneously fermented cassava batches, four strains were selected on the basis of their enzymatic activities and acid production. all were identified as lactobacillus plantarum and were used as st ... | 2000 | 10857544 |
| predictive microbiology and food safety. | the evaluation of risk in food safety requires knowledge of the probability that microbial population sizes will not exceed defined levels. this probability is evaluated assuming that the growth of the microbial population can be described by the gompertz equation with the variance of growth depending on the population size. it is shown that the probability density associated with this phenomenon is skewed, so that the risk of a high microbial population is greater than that which would be estim ... | 2000 | 10868679 |
| isolation of tannin-degrading lactobacilli from humans and fermented foods. | lactobacilli with tannase activity were isolated from human feces and fermented foods. a pcr-based taxonomic assay revealed that the isolates belong to lactobacillus plantarum, l. paraplantarum, and l. pentosus. additional studies on a range of lactobacillus species from established culture collections confirmed that this enzymatic activity is a phenotypic property common to these three species. | 2000 | 10877812 |
| antitumor effect of heat-killed lactobacillus plantarum l-137 through restoration of impaired interleukin-12 production in tumor-bearing mice. | we have previously reported that heat-killed lactobacillus plantarum l-137 is a potent inducer of interleukin-12 (il-12) in vivo as well as in vitro in mice. in order to develop effective usage of l. plantarum l-137 for tumor immunotherapy, we examined its antitumor effect against dba/2 mice inoculated with syngenic p388d1 tumor cells in different treatment schedules. daily injection of l. plantarum l-137 from the day of tumor inoculation induced a steep increase in plasma il-12 only after the f ... | 2000 | 10881695 |
| combined effect of sourdough lactic acid bacteria and additives on bread firmness and staling. | the effect of various sourdoughs and additives on bread firmness and staling was studied. compared to the bread produced with saccharomyces cerevisiae 141, the chemical acidification of dough fermented by s. cerevisiae 141 or the use of sourdoughs increased the volume of the breads. only sourdough fermentation was effective in delaying starch retrogradation. the effect depended on the level of acidification and on the lactic acid bacteria strain. the effect of sourdough made of s. cerevisiae 141 ... | 2000 | 10898663 |
| enzyme, bacterial inoculant, and formic acid effects on silage composition of orchardgrass and alfalfa. | we evaluated the effects of cellulase (from trichoderma longibrachiatum) application rates on neutral detergent fiber (ndf) concentration and fermentation products of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages harvested with decreasing dry matter (dm) digestibility. additionally, the impacts of inoculant (lactobacillus plantarum and pediococcus cerevisiae), pectinase (from aspergillus niger), or formic acid on silage composition were studied. forages wilted to ... | 2000 | 10908057 |
| persistence of lactobacillus plantarum dsm 9843 on human tonsillar surface after oral administration in fermented oatmeal gruel. a pilot study. | the occurrence of lactobacillus plantarum dsm 9843 on tonsillar scrapings was studied after single-dose administration. six healthy volunteers gargled 100 ml of fermented oatmeal gruel containing 2 x 10(11) colony forming units (cfu) of lb. plantarum dsm 9843 for 2 min and then swallowed it. two healthy volunteers drank 50 ml fermented oatmeal gruel (containing 1 x 10(11) cfu of lb. plantarum dsm 9843) mixed with 50 ml fruit juice, and in another experiment, 5 ml fermented oatmeal gruel (contain ... | 2000 | 10909023 |
| behavior of listeria monocytogenes in pasteurized milk during fermentation with lactic acid bacteria. | the behavior of listeria monocytogenes in pasteurized milk during fermentation with starter and nonstarter lactic acid bacteria was investigated. pasteurized milk was co-inoculated with approximately 10(4) cfu/ml of l. monocytogenes and 10(6) cfu/ml of lactococcus lactis, lactococcus cremoris, lactobacillus plantarum, lactobacillus bulgaricus, or streptococcus thermophilus. inoculated milks were incubated at 30 degrees c or 37 degrees c for 24 to 72 h. listeria monocytogenes survived and also gr ... | 2000 | 10914660 |
| comparative characterization of complete and truncated forms of lactobacillus amylovorus alpha-amylase and role of the c-terminal direct repeats in raw-starch binding. | two constructs derived from the alpha-amylase gene (amya) of lactobacillus amylovorus were expressed in lactobacillus plantarum, and their expression products were purified, characterized, and compared. these products correspond to the complete (amya) and truncated (amyadelta) forms of alpha-amylase; amyadelta lacks the 66-kda carboxyl-terminal direct-repeating-unit region. amya and amyadelta exhibit similar amylase activities towards a range of soluble substrates (amylose, amylopectin and alpha ... | 2000 | 10919790 |
| knockout of the p-coumarate decarboxylase gene from lactobacillus plantarum reveals the existence of two other inducible enzymatic activities involved in phenolic acid metabolism. | lactobacillus plantarum nc8 contains a pdc gene coding for p-coumaric acid decarboxylase activity (pdc). a food grade mutant, designated lpd1, in which the chromosomal pdc gene was replaced with the deleted pdc gene copy, was obtained by a two-step homologous recombination process using an unstable replicative vector. the lpd1 mutant strain remained able to weakly metabolize p-coumaric and ferulic acids into vinyl derivatives or into substituted phenyl propionic acids. we have shown that l. plan ... | 2000 | 10919793 |
| isolation and identification of tetracycline resistant lactic acid bacteria from pre-packed sliced meat products. | in recent years, the food chain has been recognised as one of the main routes for transmission of antibiotic resistant bacteria between the animal and human population. in this regard, the current study aimed to investigate if tetracycline resistant (tetr) lactic acid bacteria (lab) are present in ready-to-eat modified atmosphere packed (map) sliced meat products including fermented dry sausage, cooked chicken breast meat and cooked ham. from population graphs based on doubling tetracycline conc ... | 2000 | 10930081 |
| functional analysis of promoters involved in quorum sensing-based regulation of bacteriocin production in lactobacillus. | bacteriocin production in lactobacillus sake lth673 involves at least four operons: a regulatory operon (sppipkr); two operons encoding bacteriocins and their immunity proteins (sppaia and orfx); and an operon needed for secretion (sppte). we show here that the response regulator encoded by sppr in l. sake lth673, as well as the homologous response regulators encoded by plnc and plnd in lactobacillus plantarum c11, bind to characteristic repeats found in the -80 to -40 regions of spp operons. th ... | 2000 | 10931355 |
| biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'almagro' eggplants. | a total of 149 strains of lactic acid bacteria isolated from the spontaneous fermentation of 'almagro' eggplants were characterized and identified. of the isolates, 148 were determined as belonging to the genus lactobacillus. a coccoid, gram-positive database-negative isolate was obtained in the early stages of fermentation. the lactobacillus strains were divided into six groups based on sugar fermentation patterns and other physiological and morphological characteristics, and were identified as ... | 2000 | 10946834 |
| changes in cspl, cspp, and cspc mrna abundance as a function of cold shock and growth phase in lactobacillus plantarum. | an inverse pcr strategy based on degenerate primers has been used to identify new genes of the cold shock protein family in lactobacillus plantarum. in addition to the two previously reported cspl and cspp genes, a third gene, cspc, has been cloned and characterized. all three genes encode small 66-amino-acid proteins with between 73 and 88% identity. comparative northern blot analyses showed that the level of cspl mrna increases up to 17-fold after a temperature downshift, whereas the mrna leve ... | 2000 | 10960094 |
| effects of high pressure on survival and metabolic activity of lactobacillus plantarum tmw1.460. | the application of high pressure (hp) for food preservation requires insight into mechanisms of hp-mediated cell injury and death. the hp inactivation in model beer of lactobacillus plantarum tmw1.460, a beer-spoiling organism, was investigated at pressures ranging from 200 to 600 mpa. surviving cells were characterized by determination of (i) cell viability and sublethal injury, (ii) membrane permeability to the fluorescent dyes propidium iodide (pi) and ethidium bromide (eb), (iii) metabolic a ... | 2000 | 10966416 |
| purification and characterization of novel antifungal compounds from the sourdough lactobacillus plantarum strain 21b. | sourdough lactic acid bacteria were selected for antifungal activity by a conidial germination assay. the 10-fold-concentrated culture filtrate of lactobacillus plantarum 21b grown in wheat flour hydrolysate almost completely inhibited eurotium repens ibt18000, eurotium rubrum ftdc3228, penicillium corylophilum ibt6978, penicillium roqueforti ibt18687, penicillium expansum idm/fs2, endomyces fibuliger ibt605 and idm3812, aspergillus niger ftdc3227 and idm1, aspergillus flavus ftdc3226, monilia s ... | 2000 | 10966432 |
| mechanism of elementary catalytic steps of pyruvate oxidase from lactobacillus plantarum. | single steps in the catalytic cycle of pyruvate oxidase from lactobacillus plantarum have been characterized kinetically and mechanistically by stopped-flow in combination with kinetic solvent isotope effect studies. reversible substrate binding of pyruvate occurs with an on-rate of 6.5 x 10(4) m(-1) s(-1) and an off-rate of pyruvate of 20 s(-1). decarboxylation of the intermediate lactyl-thdp and the reduction of fad which consists of two consecutive single electron-transfer steps from hethdp t ... | 2000 | 10978159 |
| comparative genomics of the late gene cluster from lactobacillus phages. | three prophage sequences were identified in the lactobacillus johnsoni strain ncc533. prophage lj965 predicted a gene map very similar to those of pac-site streptococcus thermophilus phages over its dna packaging and head and tail morphogenesis modules. sequence similarity linked the putative dna packaging and head morphogenesis genes at the protein level. prophage lj965/s. thermophilus phage sfi11/lactococcus lactis phage tp901-1 on one hand and lactobacillus delbrueckii phage ll-h/lactobacillu ... | 2000 | 10998330 |
| urea hydrogen peroxide reduces the numbers of lactobacilli, nourishes yeast, and leaves no residues in the ethanol fermentation. | urea hydrogen peroxide (uhp) at a concentration of 30 to 32 mmol/liter reduced the numbers of five lactobacillus spp. (lactobacillus plantarum, l. paracasei, lactobacillus sp. strain 3, l. rhamnosus, and l. fermentum) from approximately 10(7) to approximately 10(2) cfu/ml in a 2-h preincubation at 30 degrees c of normal-gravity wheat mash at approximately 21 g of dissolved solids per ml containing normal levels of suspended grain particles. fermentation was completed 36 h after inoculation of sa ... | 2000 | 11010858 |
| adaptation of the nisin-controlled expression system in lactobacillus plantarum: a tool to study in vivo biological effects. | the potential of lactic acid bacteria as live vehicles for the production and delivery of therapeutic molecules is being actively investigated today. for future applications it is essential to be able to establish dose-response curves for the targeted biological effect and thus to control the production of a heterologous biopeptide by a live lactobacillus. we therefore implemented in lactobacillus plantarum ncimb8826 the powerful nisin-controlled expression (nice) system based on the autoregulat ... | 2000 | 11010894 |
| cluster analysis, richness and biodiversity indexes derived from denaturing gradient gel electrophoresis fingerprints of bacterial communities demonstrate that traditional maize fermentations are driven by the transformation process. | the bacterial communities of maize fermented foods (pozol, poto-poto and ogi) from mexico, congo and benin was compared using a culture-independent approach [denaturing gradient gel electrophoresis (dgge) analysis of total dna]. foods produced following the same flow chart (i) grouped in distinct clusters, (ii) shared similar richness and biodiversity indexes and (iii) exhibited a high intra-specific variability. structural biodiversity was higher in pozol samples, probably due to oxic condition ... | 2000 | 11014526 |
| effects of lactic acid bacteria in inoculants on changes in amino acid composition during ensilage of sterile and non-sterile ryegrass. | a study was carried out on the changes occurring in the amino acid fraction of a hybrid ryegrass during ensilage in laboratory-scale silos to help to establish the relative roles of plant and microbial proteases on protein degradation in the silo. herbage treatments included (i) normal grass without treatment (ii) lambda-irradiated grass (sterile) without treatment (iii) sterile, inoculated with a strain of lactobacillus plantarum and (iv) sterile, inoculated with a strain of lactobacillus parac ... | 2000 | 11021576 |
| lactobacillus arizonensis sp. nov., isolated from jojoba meal. | five strains of simmondsin-degrading, lactic-acid-producing bacteria were isolated from fermented jojoba meal. these isolates were facultatively anaerobic, gram-positive, non-motile, non-spore-forming, homofermentative, rod-shaped organisms. they grew singly and in short chains, produced lactic acid but no gas from glucose, and did not exhibit catalase activity. growth occurred at 15 and 45 degrees c. all strains fermented cellobiose, d-fructose, d-galactose, d-glucose, lactose, maltose, d-manni ... | 2000 | 11034490 |
| intake, digestibility, and composition of orchardgrass and alfalfa silages treated with cellulase, inoculant, and formic acid fed to lambs. | the objectives of this study were to determine the effect of a cellulase (from trichoderma longibrachiatum) alone or combined with a bacterial inoculant (lactobacillus plantarum and pediococcus cerevisiae) or formic acid on composition, intake, and digestibility of orchardgrass (dactylis glomerata l.) and alfalfa (medicago sativa l.) silages. orchardgrass and alfalfa were harvested at the early heading stage and at the early bloom stage of maturity and wilted to approximately 22 and 32% dm, resp ... | 2000 | 11063325 |
| molecular analysis of mutated lactobacillus acidophilus promoter-like sequence p15. | the promoter-like sequence p15 that was previously cloned from the chromosome of lactobacillus acidophilus atcc 4356 is active in lactobacillus reuteri, lactobacillus plantarum, lactobacillus acidophilus, and escherichia coli, but not in lactococcus lactis. n-methyl-n-nitroso-n-guanidine (mnng) mutagenesis of p15 was used to select for a promoter active in l. lactis mg1363. molecular analysis of the mutated promoter (designated p16) revealed a 90 bp deletion and a t-->a transversion. this deleti ... | 2000 | 11068681 |
| inducible metabolism of phenolic acids in pediococcus pentosaceus is encoded by an autoregulated operon which involves a new class of negative transcriptional regulator. | pediococcus pentosaceus displays a substrate-inducible phenolic acid decarboxylase (pad) activity on p-coumaric acid. based on dna sequence homologies between the three pads previously cloned, a dna probe of the lactobacillus plantarum pdc gene was used to screen a p. pentosaceus genomic library in order to clone the corresponding gene of this bacteria. one clone detected with this probe displayed a low pad activity. subcloning of this plasmid insertion allowed us to determine the part of the in ... | 2000 | 11073918 |
| inhibition of bacillus cereus atcc 14579 by plantaricin ug1 in vitro and in food. | the inhibition of bacillus cereus atcc 14579 viable growth by lactobacillus plantarum ug1 bacteriocin (plantaricin ug1) in vitro and in food (pasteurized milk and minced meat) was studied. the inhibitory effect against b. cereus food-borne pathogen noticed in this study was due to plantaricin ug1, but not due to lactic acid produced by the l. plantarum ug1 culture. plataricin ug1 negative clone did not affect viable growth of b. cereus in both broth and meat or pasteurized milk. the inhibitory e ... | 2000 | 11075381 |
| evaluation of numerical analyses of rapd and api 50 ch patterns to differentiate lactobacillus plantarum, lact. fermentum, lact. rhamnosus, lact. sake, lact. parabuchneri, lact. gallinarum, lact. casei, weissella minor and related taxa isolated from kocho and tef. | the study was carried out to assess the agreement of api 50 ch fermentation data of food lactobacilli with their rapd profiles to determine whether the system could be used alone as a reliable taxonomic tool for this genus. | 2000 | 11123470 |
| molecular characterization of lactobacillus plantarum genes for beta-ketoacyl-acyl carrier protein synthase iii (fabh) and acetyl coenzyme a carboxylase (accbcda), which are essential for fatty acid biosynthesis. | genes for subunits of acetyl coenzyme a carboxylase (acc), which is the enzyme that catalyzes the first step in the synthesis of fatty acids in lactobacillus plantarum l137, were cloned and characterized. we identified six potential open reading frames, namely, manb, fabh, accb, accc, accd, and acca, in that order. nucleotide sequence analysis suggested that fabh encoded beta-ketoacyl-acyl carrier protein synthase iii, that the accb, accc, accd, and acca genes encoded biotin carboxyl carrier pro ... | 2001 | 11133475 |
| probiotics in foods not containing milk or milk constituents, with special reference to lactobacillus plantarum 299v. | lactic acid fermentation is the simplest and safest way of preserving food and has probably always been used by humans. species such as lactobacillus plantarum, lactobacillus rhamnosus, lactobacillus paracasei, lactobacillus acidophilus, and lactobacillus salivarius are common in the human mucosa, from the mouth to the rectum. in food, l. paracasei and l. rhamnosus are usually associated with dairy products whereas l. plantarum is found in fermented foods of plant origin. a probiotic food produc ... | 2001 | 11157345 |
| reduction of fecal shedding of enterohemorrhagic escherichia coli o157:h7 in lambs by feeding microbial feed supplement. | enterohemorrhagic escherichia coli o157:h7, an emerging food-borne pathogen, has been implicated in several outbreaks in the us. ruminants, including cattle, sheep and deer are reservoirs of e. coli o157:h7 and fecal shedding of the pathogen forms the vehicle of entry into the human food chain. we studied the efficacy of lactobacillus acidophilus, streptococcus faecium, a mixture of l. acidophilus and s. faecium and a mixture of l. acidophilus, s. faecium, lactobacillus casei, lactobacillus ferm ... | 2001 | 11163713 |
| rapid pcr-based procedure to identify lactic acid bacteria: application to six common lactobacillus species. | the goal of this study was to develop a method allowing rapid identification of the lactic acid bacteria strains in use in the laboratory (lactobacillus plantarum ncimb8826; l. fermentum kld; l. reuteri 100-23; l. salivarius ucc43321; l. paracasei lbtgs1.4; l. casei atcc393), based on pcr amplification of 16s rna coding sequences. first, specific forward oligonucleotides were designed in the variable regions of 16s rna coding sequences of six lactobacillus strains. the reverse oligonucleotide wa ... | 2001 | 11165343 |
| biogenic amine production by lactobacillus. | the aim of this work was to demonstrate that strains of lactobacillus may be able to produce putrescine and agmatine from one of the major amino acids present in fruit juices and wine, arginine, and from amino acid-derived ornithine. | 2001 | 11168717 |
| selective sensitization of bacteria to peroxide damage associated with fluoride inhibition of catalase and pseudocatalase. | fluoride and sulfide are known inhibitors of heme catalases in acid environments. staphylococcus aureus h cells were found to be sensitized by fluoride or sulfide to h2o2 killing at acid ph values in the range of 3.5 to 4.0, and catalase activity was reduced concomitantly. in contrast, fluoride had little effect on h2o2 killing of streptococcus mutans gs-5, which has fluoride-insensitive peroxidase activity, but still is more sensitive to h2o2 than is s. aureus in the absence of fluoride. fluori ... | 2001 | 11169136 |
| mucosal immune responses and protection against tetanus toxin after intranasal immunization with recombinant lactobacillus plantarum. | the use of live microorganisms as an antigen delivery system is an effective means to elicit local immune responses and thus represents a promising strategy for mucosal vaccination. in this respect, lactic acid bacteria represent an original and attractive approach, as they are safe organisms that are used as food starters and probiotics. to determine whether an immune response could be elicited by intranasal delivery of recombinant lactobacilli, a lactobacillus plantarum strain of human origin ... | 2001 | 11179325 |
| probiotic therapy fails to improve gut permeability in a hapten model of colitis. | studies in clinical and experimental inflammatory bowel disease (ibd) have shown disturbances in intestinal bacterial flora with an increase in potentially pathogenic and a decrease in protective organisms. it was hypothesized that lactobacillus plantarum species 299 (lp299), a probiotic, would ameliorate colitis and improve intestinal permeability in experimental colitis. this study investigated the effect of lp299 in the trinitrobenzenesulfonic acid/ethanol (tnbs/e) rat model of colitis. | 2000 | 11199365 |
| identification of lactic acid bacteria constituting the predominating microflora in an acid-fermented condiment (tempoyak) popular in malaysia. | tempoyak is a traditional malaysian fermented condiment made from the pulp of the durian fruit (durio zibethinus). salt is sometime added to proceed fermentation at ambient temperature. in various samples obtained from night markets, lactic acid bacteria (lab) were the predominant microorganisms, ranging from log 8.4 to log 9.2 cfu g(-1). no other microorganisms were present to such a level. these samples contained reduced amount of saccharose, glucose and fructose but increased amount of d- and ... | 2001 | 11205946 |
| membrane damage and enzyme inactivation of lactobacillus plantarum by high pressure co2 treatment. | physiological changes of lactobacillus plantarum (kfri 815) by high pressure co2 treatment were investigated to examine the relevance to microbial inactivation. characteristic properties of the cells measured in this study included salt tolerance, release of uv-absorbing substances, mg and k ions, proton permeability, glycolysis, h+-atpase and constitutive enzymes, and dye uptake. the cells treated with high pressure co2 of 7 mpa at 30 degrees c for 10 min showed the irreversible cellular membra ... | 2001 | 11205950 |
| expression in escherichia coli of native and chimeric phenolic acid decarboxylases with modified enzymatic activities and method for screening recombinant e. coli strains expressing these enzymes. | four bacterial phenolic acid decarboxylases (pad) from lactobacillus plantarum, pediococcus pentosaceus, bacillus subtilis, and bacillus pumilus were expressed in escherichia coli, and their activities on p-coumaric, ferulic, and caffeic acids were compared. although these four enzymes displayed 61% amino acid sequence identity, they exhibit different activities for ferulic and caffeic acid metabolism. to elucidate the domain(s) that determines these differences, chimeric pad proteins were const ... | 2001 | 11229892 |
| [molecular structure of the locus for sucrose utilization by lactobacillus plantarum: comparison with pediococcus pentosaceus]. | structure of the sucrose utilization locus in a lactobacillus plantarum type strain was studied using pcr and southern hybridization. restriction map analysis revealed its high similarity to the sequenced sucrose utilization locus of pediococcus pentosaceus psrq1. the l. plantarum locus proved containing oppositely oriented scra and the scrbragl operon, but not agas. the l. plantarum sucrase gene (scrb) was partly sequenced. a higher (98.6%) homology was revealed between scrb than between the 16 ... | 2001 | 11234378 |
| plantaricin w from lactobacillus plantarum belongs to a new family of two-peptide lantibiotics. | plantaricin w (plw) is a new two-peptide bacteriocin, from lactobacillus plantarum, which inhibits a large number of gram-positive bacteria. the two peptides, plwalpha (comprising 29 residues) and plwbeta (comprising 32 residues), were isolated from the culture supernatants and characterized. the individual peptides had low antimicrobial activity but acted synergistically, and synergism was seen at all mixing ratios tested. the data indicate that the two peptides work in a 1:1 ratio. chemical an ... | 2001 | 11238971 |
| lactobacillus plantarum reduces infection of pancreatic necrosis in experimental acute pancreatitis. | infection is the commonest cause of death in acute pancreatitis. early reduction of commensal flora (particularly lactobacillus species) and, at the same time, overgrowth of enterobacteriaceae, especially escherichia coli, have recently been described during acute pancreatitis. lactobacillus plantarum has been shown to be effective in reducing the egress of endotoxin and microbial translocation in several experimental models such as chemically induced hepatitis and ulcerative colitis. | 2001 | 11244259 |
| the microbiology of south african traditional fermented milks. | a total of 15 samples of traditional fermented milk were collected from individual households in south africa and namibia. lactic acid bacteria dominated the microflora of these samples, especially the genera leuconostoc, lactococcus and lactobacillus. other groups identified included pyogenic streptococci and enterococci. the dominant lactococci species was lactococcus lactis subsp. lactis. eighty-three percent of the leuconostoc isolates were identified as leuconostoc mesenteroides subsp. dext ... | 2001 | 11246902 |
| detection and preliminary characterization of a bacteriocin (plantaricin 35d) produced by a lactobacillus plantarum strain. | lactic acid bacteria (134) from italian sausages were tested for the production of antimicrobial substances (bacteriocins). six percent of these showed antibacterial activity against one or several closely related microorganisms used as indicators. lactobacillus plantarum 35d in particular produced a bacteriocin of high activity (320 au ml(-1)) and a wide range of antimicrobial activity including s. aureus, l. monocytogenes, and a. hydrophila. the bacteriocin withstood heating at 80 degrees c fo ... | 2001 | 11252503 |
| lactic acid bacterial diversity in the traditional mexican fermented dough pozol as determined by 16s rdna sequence analysis. | the lactic acid bacteria diversity of pozol, a mexican fermented maize dough, was studied using a total dna extraction and purification procedure and pcr amplification of 16s rdna for gram-positive and related bacterial groups. thirty-six clones were obtained and sequenced to 650 nucleotides. these partial sequences were identified by submission to the non-redundant nucleotide database of ncbi. the identified sequences were aligned with reference sequences of the closest related organisms. this ... | 2001 | 11252506 |
| identification of a non-haem catalase in salmonella and its regulation by rpos (sigmas). | we report the identification and functional analysis of katn, a gene encoding a non-haem catalase of salmonella enterica serotype typhimurium. katn, which is not present in escherichia coli, is located between the ycigfe and ycid e. coli homologues in the salmonella genome. its predicted protein product has a molecular weight of 31 826 da and is similar to the mn-catalases of lactobacillus plantarum and thermus spp. its product, katn, was visualized as a 37 kda protein in e. coli maxicells. a ka ... | 2001 | 11260470 |
| lactobacillus plantarum phytase activity is due to non-specific acid phosphatase. | microbial phytases suitable for food fermentations could be obtained from lactic acid bacteria isolated from natural vegetable fermentations. phytase activity was evaluated for six lactic acid bacteria cultures. although the highest activity was found for lactobacillus plantarum, the phytase activity was very low. further characterization of the enzyme with phytate-degrading activity showed a molecular weight of 52 kda and an optimum activity at ph 5.5 and 65 degrees c. enzyme activity was due t ... | 2001 | 11264749 |
| taxonomy of obligately homofermentative and facultatively heterofermentative lactobacilli in pig faeces. | identification and characterization of obligately homofermentative and facultatively heterofermentative strains of lactobacillus spp. isolated from the faeces of pigs that had been raised under different conditions. | 2001 | 11264753 |
| study of inactivation of lactobacillus plantarum in orange-carrot juice by means of pulsed electric fields: comparison of inactivation kinetics models. | the inactivation kinetics of lactobacillus plantarum was studied in orange-carrot juice using high intensity pulsed electric fields. the results indicated that under the treatment conditions applied, 28.6, 32.0, and 35.8 kv/cm and treatment times ranging from 10.2 to 46.3 micros, the inactivation of l. plantarum obtained was up to 2.5 decimal reductions. experimental and literature data were fitted to bigelow, hülsheger et al. and peleg models and to weibull frequency distribution function. weib ... | 2001 | 11271778 |
| [effect of probiotic administration on colic anastomosis healing]. | colic anastomoses are still affected by a high incidence of leakage. we speculate that a supply of fibres and probiotic bacteria improves the healing of colic anastomoses due to a higher production of short-chain fatty acids. these are known to improve the anastomotic healing of colic sutures. sixty lewis rats, weighing from 250 g to 350 g, were divided into 6 groups. groups a + a1 were fed with a low-fibre diet (less than 0.1%), groups b + b1 with normal rat chow and groups c + c1 with normal r ... | 2001 | 11280827 |
| selection and characterization of mixed starter cultures for lactic acid fermentation of carrot, cabbage, beet and onion vegetable mixtures. | an evaluation of various lactic acid bacteria (lab) for the fermentation of cabbage, carrot and beet-based vegetable products was carried out. as part of a screening process, the growth of 15 cultures in a vegetable juice medium (vjm) was characterized by automated spectrophotometry. acidification patterns as well as viability during storage of the lab were also established. there were greater differences between the pure cultures than the mixed ones with respect to growth in vjm and viability d ... | 2001 | 11294348 |
| reaction kinetic interpretation of heat destruction influenced by environmental factors. | based on reaction kinetics and thermodynamic considerations a mathematical model was developed to describe the thermal death rate of microbes as a function of environmental factors such as temperature, water activity, ph and redox potential. the mathematical model is discussed and validated by fitting to data sets of heat destruction of lactobacillus brevis and lactobacillus plantarum. the mathematical analysis of the kinetic model and the parameters of the model fitting demonstrate its applicab ... | 2001 | 11294350 |
| stochastic modelling of the growth of a microbial population under changing temperature regimes. | the application of models of microbial growth to the design of food safety systems requires consideration of the effect of arbitrary changes in external variables on growth of bacteria. in particular, the effect of changes in external variables, such as temperature, on the probability that the microbial population size will not exceed acceptable levels at a given time needs to be predicted. this paper presents a method of calculating the time-dependent probability distribution of the microbial p ... | 2001 | 11294353 |
| differential viable count of mixed starter cultures of lactic acid bacteria in doughs by using modified chalmers medium. | the modified chalmers medium appeared as quite suitable for counting a mixed population consisting of different species of lactic acid bacteria used as starter in breadmaking. selected strains of lactobacillus plantarum, leuconostoc mesenteroides subsp. mesenteroides, lactobacillus sanfranciscensis, enterococcus faecalis together with saccharomyces cerevisiae could be easily differentiated and counted with an acceptable recovery in comparison with reference media. | 2001 | 11297368 |
| characterization of the lactic acid bacteria in ewe's milk and cheese from northwest argentina. | indigenous lactic acid bacteria in ewe's milk and artisanal cheese were studied in four samples of fresh raw milk and four 1-month-old cheeses from the provinces of northwest argentina. mean growth counts on m17, mrs, and mse agar media did not show significant differences (p < 0.05) in raw milk and cheeses. isolates of lactic acid bacteria from milk were identified as enterococcus (48%), lactococci (14%), leuconostocs (8%), and lactobacilli (30%). all lactococci were identified as lactococcus l ... | 2001 | 11307898 |
| characterization of non-starter lactic acid bacteria from italian ewe cheeses based on phenotypic, genotypic, and cell wall protein analyses. | non-starter lactic acid bacteria (nslab) were isolated from 12 italian ewe cheeses representing six different types of cheese, which in several cases were produced by different manufacturers. a total of 400 presumptive lactobacillus isolates were obtained, and 123 isolates and 10 type strains were subjected to phenotypic, genetic, and cell wall protein characterization analyses. phenotypically, the cheese isolates included 32% lactobacillus plantarum isolates, 15% l. brevis isolates, 12% l. para ... | 2001 | 11319075 |
| dynamics of the microbial community responsible for traditional sour cassava starch fermentation studied by denaturing gradient gel electrophoresis and quantitative rrna hybridization. | the microbial community developing during the spontaneous fermentation of sour cassava starch was investigated by cultivation-independent methods. denaturing gradient gel electrophoresis (dgge) of partially amplified 16s rdna followed by sequencing of the most intense bands showed that the dominant organisms were all lactic acid bacteria (lab), mainly close relatives of bifidobacterium minimum, lactococcus lactis, streptococcus sp., enterococcus saccharolyticus and lactobacillus plantarum., clos ... | 2001 | 11322700 |
| characterization of a lytic lactobacillus plantarum bacteriophage and molecular cloning of a lysin gene in escherichia coli. | bacteriophage sc921, which can infect lactobacillus plantarum specifically, was isolated from a fermented vegetable source, kimchi. this phage is active against six of 11 strains of l. plantarum tested as hosts. morphologically, it has an isometric head (60 nm in diameter) and a non-contractile tail (260 nm long and 9-11 nm wide), indicating that it belongs to bradley's group b or the siphoviridae family according to the international committee on taxonomy of viruses (ictv). the bouyant density ... | 2001 | 11322702 |
| regulation of bacteriocin production in lactobacillus plantarum depends on a conserved promoter arrangement with consensus binding sequence. | bacteriocin production in lactobacillus plantarum c11 is regulated by a three-component signal transduction system comprising a peptide pheromone (plna), a histidine protein kinase (plnb), and two homologous response regulators (rrs; plnc and plnd). both rrs are dna-binding proteins that bind to promoter-proximal elements in the pln regulon. the binding site for the two regulators consists of two 9-bp direct repeats, that conform to the consensus sequence 5'-tacgttaat-3', and the repeats are sep ... | 2001 | 11370867 |
| the effect of lactobacillus buchneri and other additives on the fermentation and aerobic stability of barley silage. | whole-plant barley (39.4% dry matter) was treated with various chemical and biological additives to assess their effects on silage fermentation and aerobic stability. treatments were untreated forage, forage treated with several amounts of lactobacillus buchneri and enzymes (l. buchneri at 1 x 10(5), 5 x 10(5), and 1 x 10(6) cfu/g of fresh forage), forage treated with an inoculant containing (lactobacillus plantarum, pediococcus pentosaceus, propionibacterium freudenreichii, and enzymes), or for ... | 2001 | 11384041 |
| characteristics and significance of yeasts' involvement in cassava fermentation for 'fufu' production. | six different strains of yeast, namely candida krusei, c. tropicalis, pichia saitoi, saccharomyces cerevisiae, p. anomala and zygosaccharomyces bailii were found present in cassava-fermenting water in the early part of the fermentation. the latter part of the fermentation was dominated in all cases by three strains of yeast namely c. krusei, c. tropicalis and z. bailii. all the yeast strains exhibited amylolytic capabilities while none was able to produce cellulase. all the strains except zygosa ... | 2001 | 11393690 |
| accumulation of manganese in neisseria gonorrhoeae correlates with resistance to oxidative killing by superoxide anion and is independent of superoxide dismutase activity. | as a facultative aerobe with a high iron requirement and a highly active aerobic respiratory chain, neisseria gonorrhoeae requires defence systems to respond to toxic oxygen species such as superoxide. it has been shown that supplementation of media with 100 microm mn(ii) considerably enhanced the resistance of this bacterium to oxidative killing by superoxide. this protection was not associated with the superoxide dismutase enzymes of n. gonorrhoeae. in contrast to previous studies, which sugge ... | 2001 | 11401721 |
| lactobacillus casei and lactobacillus plantarum initiate catabolism of methionine by transamination. | to study the ability of lactobacillus casei and lact. plantarum strains to convert methonine to cheese flavour compounds. | 2001 | 11412327 |
| the functional ccpa gene is required for carbon catabolite repression in lactobacillus plantarum. | we report the characterization of the ccpa gene of lactobacillus plantarum, coding for catabolite control protein a. the gene is linked to the pepq gene, encoding a proline peptidase, in the order ccpa-pepq, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. two ccpa transcription start sites corresponding to two functional promoters were found, expression from the upstream promoter being autogenously regulated through a catabolite-responsive element ... | 2001 | 11425700 |
| membrane permeabilization in relation to inactivation kinetics of lactobacillus species due to pulsed electric fields. | membrane permeabilization due to pulsed electric field (pef) treatment of gram-positive lactobacillus cells was investigated by using propidium iodide uptake and single-cell analysis with flow cytometry. electric field strength, energy input, treatment time, and growth phase affected membrane permeabilization of lactobacillus plantarum during pef treatment. a correlation between pef inactivation and membrane permeabilization of l. plantarum cells was demonstrated, whereas no relationship was obs ... | 2001 | 11425727 |
| antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. | the beneficial health effects of extracts from many types of plants that are used as seasoning agents in foods and beverages have been claimed for centuries. the purpose of this study was to examine the effectiveness of selected herb and spice essential oils for control of growth and survival of microorganisms. inhibition of growth was tested by the paper disc agar diffusion method. antibiotic susceptibility discs were used as control. minimum lethal concentration (mlc) was determined by the tub ... | 2001 | 11456186 |
| three glycoproteins with antimutagenic activity identified in lactobacillus plantarum klab21. | antimutagenic substances were purified from a culture supernatant of lactobacillus plantarum klab21 cells isolated from kimchi, a korean traditional fermented vegetable, and their characteristics were investigated. the antimutagenic substances were separated into two fractions by deae-cellulose ion-exchange column chromatography, which were designated the r1 and r2 fractions. the r1 fraction was then divided into two fractions again by sephadex g200 gel filtration chromatography, and the fractio ... | 2001 | 11472917 |